45results about How to "Reduce the number of bacteria" patented technology

The invention relates to technology for fermenting and decomposing protein feed with protease in two steps by using probiotics, which is characterized in that the protein is fermented and decomposed with protease of aerobic fermentation matter in the anaerobic condition, soybean dregs, cotton dregs, vegetable dregs meal, wheat bran and other vegetable protein raw materials are sterilized at high temperature and then are aerobically fermented for 24-36h, feather meal and other animal protein raw materials are aerobically fermented for 20-26h, the inoculating temperature is controlled at 60-80 DEG C, the fermented materials are not dried and are directly mixed with fresh raw materials, corn steep liquor and other nutrient solution are added or not added; lactic acid bacillus are inoculated to the mixture, and water is supplemented to the mixture until the water content of the mixture reaches 40-60 percent, the mixture is compacted and sealed to be anaerobically fermented for 1-3d above 20 DEG C or 2-5d below 20 DEG C, and the mixture is dried after being fermented and decomposed with protease.

The invention discloses an agricultural composite microbial preparation, respective zymocyte solutions of soja bean legume bacteria, round brown azotobacteria, bacillus megaterium and colloidal substance bacillus are respectively adsorbed on the adsorbing agent according to the proportionality of 15-20 % by weight to prepare soja bean legume bacteria, round brown azotobacteria, bacillus megaterium and colloidal substance bacillus, which are compounded to the agricultural composite microbial preparation; the invention adopts the composition of four kinds of bacterial seeds, also considers the requirement ofnitrogen, phosphor and kalium nutrients for plant, it is proved by the field experiment, the obvious yield increasing and quality raising actions for multiple corps are provided; the method accelerates the growth respectively adopting the controls of air flow of microorganism, temperature and inoculating amount during fermentation, enables the fermentation periods of the four kinds of composite bacterial series to be shortened, thereby fermenting at the same time and the terminating at the same time.

The invention discloses a process-controlled rapid fermentation processing method of pure strains of Liuyang salt black beans. The method comprises the following steps: removing impurities of fresh black beans; soaking; steaming at high pressure to ensure that proteins are denatured properly and cooling; inoculating aspergillus oryzae for enlarge cultivation and uniformly stirring for fermentation starter propagation; naturally cooling prepared cooked yeast, wherein protease activity is 800-1,200 unit/g dry basis (based on neutral protease); adding hot water and stirring; rapidly draining and adding small amount of salt or white spirit; uniformly stirring and compacting and putting into a sealing interlayer tank for heat-preservation fermentation so that fermented amino acid nitrogen is up to more than 0.55 percent; finally, dehydrating and drying by using continuous flow belt type drying equipment so that the water content of the product is less than 15 percent, the amino acid nitrogen is more than 0.85 percent, and the bacterial quantity is less than 103 cfu/g. The quality of the product is stable, the key procedures of each process are controlled in an interlocking mode and have synergistic effects, and the process from material input to acquisition of finished product only needs 15-25 days. The method is short in process period and stable in quality, and a production method and a production process with high efficiency, standardization, large scale, continuity and feasibility are provided for safe and convenient low-salt or salt-free Liuyang salt black bean products.

The invention relates to a method for producing clean sterilized fresh eggs. The method comprises the following steps: (1) rejecting dirty eggs, cracked eggs, sunken eggs, soft eggs and abnormal eggs, and isolating the clean eggs suitable for being stored for a long time; (2) hot air de-dusting and sterilizing: blowing the surfaces of the egg shells from up and down as well as left and right directions with the hot air at 120-160 DEG C, keeping the air speed being 2.5m/s-10m/s, and blowing each egg for 3-30 seconds; and (3) cooling and packaging: blowing the egg shells with the air at natural temperature, cooling the egg shells till the temperature of the egg shells is room temperature, and packaging, thereby acquiring the clean sterilized fresh eggs. The clean sterilized fresh eggs prepared by using the method provided by the invention have the advantages that the water and energy are saved, the chemicals such as detergent, disinfectant, coating anti-staling agent, and the like, are unnecessary, the matters inside the eggs are pollution-free, and the method is environment-friendly and safe.

The invention provides a rapid fermentation process for preparing fermented soybeans by pure strains, which comprises the following steps: cooking and curing the soaked soybeans with a water level higher than the soaked beans; inoculating the pure mucor strains after intermediate culture; fermenting at constant temperature and humidity for 48-72 hours to obtain mucor blank; adding salt and slicedginger; compacting, sealing and fermenting in the case of voidage being less than 5%; dehydrating with circulating hot air; frying with vegetable oil at the temperature of 170-190 DEG C for 30-50 seconds; mixing with blending materials timely; hot-filling at 85-90 DEG C; pressing tightly so that the oil level is 1-1.5cm higher than the surface of the beans; and carrying out vacuum sealing to finally obtain the instant fermented soybeans after being cooled. The process provided by the invention has the advantages of scientific process design, short process cycle, good color stability, continuous industrial production, controllable process, stable quality and no addition of preservative and anti-staling agent and color fixative, and can obtain the fermented soybeans which are significantly different from the products of other types, thus providing a feasible method for safely and conveniently producing fermented soybean products.

The invention relates to a high-survival-rate azalea seedling cultivating method. The method comprises the step one of cuttage planting soil treatment, the step two of cuttage branch pretreatment, the step three of cuttage branch treatment, the step four of cuttage and the step five of management after cuttage. Before planting, liquid traditional Chinese medicine is used for treatment, the disease resistance ability of azalea seedlings is improved, and branches can be promoted for germinating and surviving; as the length of the cuttage branches is reduced, the number of bacteria on the seed branches is reduced, the mutant probability of the seedlings is reduced, the strain genuineness of azalea can be kept, the applicability of the azalea is enhanced, and the survival rate of the seedlings is increased.

The invention provides a foam cleaning composition which comprises an anionic surfactant, a nonionic surfactant, tea saponin, sodium alginate, sodium citrate, sodium chloride, a bioenzyme, betaine and the like. The foam cleaning composition can also comprise a bactericidal deodorant, an essence and a colorant. The foam cleaning composition is suitable for cleaning bedpans, and has the characteristics of high foaming speed, fine and abundant foam, long duration time and the like. The bactericidal deodorant is a pure natural antibacterial deodorant, can remove peculiar smell, has favorable bactericidal action, and is harmless to the human body and environment.

The invention discloses a traditional Chinese medicine for treating allergic rhinitis. The traditional Chinese medicine comprises the following raw materials in parts by weight: 65 parts of magnolia flower, 30 parts of astragalus root, 30 parts of radix scutellariae, 60 parts of cocklebur fruit, 30 parts of ephedra, 30 parts of bitter apricot seed, 30 parts of asarum, 36 parts of licorice, 30 parts of bighead atractylodes rhizome, 30 parts of ledebouriella root, 35 parts of mint, 35 parts of periostracum cicada, 60 parts of cordate houttuynia, 30 parts of peach kernel, and 30 parts of fragrant solomonseal rhizome. The preparation method of the traditional Chinese medicine for treating allergic rhinitis comprises the following steps: firstly, weighing all the raw materials according to the prescription amount, adding water for decocting, so as to obtain a decocted solution, secondly, concentrating the decocted solution obtained in the first step into thick paste, drying and then grinding the thick paste into powder, uniformly mixing the powder, and thirdly, checking the powder, and preparing qualified powder into a solid oral preparation. The traditional Chinese medicine has the advantages that the traditional Chinese medicine has the effects of dispelling wind pathogens and tonifying qi and strengthening exterior, can achieve the effects of treating of both symptoms and root causes and full recovery through the addition of the bitter apricot seed for promoting the dispersing function of the lung and the peach kernel for promoting blood circulation, untoward effects cannot be caused, the curative effect is stable, the total effective rate reaches 97.69 percent, and the traditional Chinese medicine is safe and reliable, can improve the curative effect and control the state of an illness, and has the good effect of improving symptoms.

The invention discloses a method for producing living paratyphoid vaccine for piglets and a product thereof. The method comprises the following steps: (1) preparing primary strains; (2) preparing secondary strains; and (3) culturing bacterial liquid: inoculating the secondary strains into a culture medium for culturing, after culturing, immediately adding a stabilizing agent which is sterilized and preheated to 37 DEG C to ensure that the vaccine contains 1.5 percent of gelatin and 5 percent of sucrose, and mixing uniformly to obtain the bacterial liquid, wherein the culture medium in the step (3) consists of a common broth medium and a synthetic medium in the volume ratio of 2:1. In the living paratyphoid vaccine for piglets produced by the method, the cultured bacteria count is over 50 billion per milliliter; the maximum cultured bacteria count is 58 billion per milliliter; and the freeze-drying death rate is reduced to 20-40 percent. The method for producing the living paratyphoid vaccine for the piglets has the advantages of high bacterial count of the living vaccine, low freeze-drying death rate, easy production, high yield, low production cost, and the like.

The invention discloses a processing technic for mixing raw materials for soy koji making. The processing technic comprises the following steps: directly mixing ripe soya just taken out of an evaporating pan and an auxiliary material to prepare the koji of soya koji making. The processing technic provided by the invention has the advantages of effectively reducing bacteria quantity of the koji, reducing the contest of bacteria and target microorganism for alimentation in the koji in koji making, and the prepared soy omagari has rich proteolytic enzyme; and proteolytic enzyme energy is high, and the protein utilization ratio in soy yeasting process is improved.

The invention discloses a tree vigor strengthening-based apple rot prevention and treatment method. The method comprises the following steps: carrying out autumn base fertilizer application in the middle of September, and on the basis of original fertilizer application, additionally applying biological organic fertilizer and potassic fertilizer to 10-year-old or older trees; smearing tree trunks and boughs with a tree nutrient solution respectively in the middle of March, in the middle of June and in the middle of August; delaying the pruning date to shorten the wound healing time and decrease the wound infection probability, and protecting pruned edges in time; spraying water to clean the garden respectively in the early March and the late October to decrease the number of bacteria on tree bodies and increase nutrition in the tree bodies; scraping off black scars, and whitewashing the tree trunks to prevent and treat freeze injury and reduce rot. Based on tree vigor strengthening, the resistance of the tree bodies is enhanced; the method is a comprehensive prevention and control method taking prevention as a main measure and black scar scraping as an auxiliary measure; in a prevention and treatment process, by increase of the nutrition in the tree bodies, cleaning of the garden with green pesticide to decrease the number of bacteria on the tree bodies and great reduction of the use amount of chemical pesticide, the aim of green prevention and control is fulfilled, the rot prevention and treatment rate is 95 percent or above, and the effect is obvious.

The invention discloses an organic microelement premix feed for a piglet and a preparing method and application of the feed. The feed comprises an iron glycine complex (chelate), basic copper chloride, a cupric glycinate complex (chelate), zinc glycine, a manganese methionine complex (chelate), a rare earth (cerium and lanthanum) chitosamine chelate preparation, a calcium iodate premixing agent, aselenium yeast premixing agent, a cobaltous sulfate premixing agent, a chromium methionine premixing agent and a carrier. The method includes the steps of adding the calcium iodate premixing agent, the cobaltous sulfate premixing agent and the chromium methionine premixing agent to part of the carrier to be mixed, and then adding the rest of raw materials and the carrier to be mixed to obtain thefeed. The feed is added to a complete formula feed according to the ratio of 1.5-2.5 kg/ton. The feed can meet the requirements of the piglet for microelements, the absorption and utilization rate isincreased, the growth performance is improved, intestinal balance is adjusted, excretion is reduced, and immunity is improved; the method is high in mixing uniformity and simple in process and savescost.

The invention discloses a spray disinfectant containing a phage composition in a breeding environment and a preparation method and application thereof. The spray disinfectant comprises phage or its composition with a total concentration greater than 1010PFU/mL and a streptococcus suis lyase LY7917 with a concentration of 100.0 [mu]g/mL according to a volume ratio of 0.1-100: 1. The phage is derived from a breeding environment and is selected from one or more of escherichia coli phage Ecp2, escherichia coli phage E20-1 and staphylococcus aureus phage. According to the spray disinfectant, a spray sterilization mode is adopted, the synergistic sterilization effect of phage and lyase can be effectively achieved, the total number of bacteria on the ground of an animal house, in air and in a feeding trough is remarkably reduced, the overall disinfection efficiency is superior to that of a chemical disinfectant glutaraldehyde decyl methylammonium bromide solution, and the spray disinfectant is an environment-friendly disinfectant for the livestock and poultry breeding environment.

The invention discloses a compound microbial inoculum which comprises the following components in parts by weight: 80 to 200 parts of leuconostoc mesenteroides and 40 to 100 parts of rhodospirillum rubrum. Simultaneously, the invention also discloses a preparation method of the microbial inoculum. According to the preparation method, two types of microbial bacteria are used as raw materials, convenience is brought to enterprises to accurately distinguish the raw materials in the process of industrialization, and the cost is greatly reduced. Because microbes have a short metabolism cycle, and cause no pollution to the surrounding environment at all, the microbes are suitable for environmental protection, cultivation and plantation industries.

The invention discloses a selenium-enriched bamboo shoot food processing method. The method comprises the following steps of a, selecting materials: selecting fresh selenium-enriched bamboo shoots having shells, cleaning away earth, and removing impurities; b, stripping shells: stripping shells and bamboo shoot coatings through a raw bamboo shoot shell stripping machine; c, performing soaking in ozone: immediately putting the bamboo shoots after shells are stripped in ozone water for soaking, wherein the soaking time is 35-45 minutes; d, performing sieving: classifying and sorting the soaked bamboo shoots according to the size, and removing old heads; e, performing soaking in clean water: putting the sorted bamboo shoots in the clean water for soaking, and performing waiting for packaging;f, performing packaging: performing weighing and packaging according to the specifications, and performing nitrogen-charged vacuum packaging on the packed bamboo shoots; and g, performing liquid nitrogen quick-freezing: performing liquid nitrogen quick-freezing on packaged products, and checking finished products. Compared with the prior art, the method disclosed by the invention has the advantages that fresh bamboo shoots are not cooked, and shell removal timely and packaging timely are performed, so that the total quantity of bacteria of finished products of the bamboo shoots is greatly reduced, the freshness of the bamboo shoots, such as color, fragrance and crispness, and selenium elements in the bamboo shoots are effectively reserved, and no preservatives and no salt are added.

The invention provides a method for collecting plasma of a live pig. The method comprises the following steps: 1, binding the whole body of the pig to be subjected to plasma collection, and fixing the bound pig to a blood collecting vehicle; 2, carrying out disinfection treatment on the selected head-neck part of the pig to be subjected to plasma collection; 3, slowly puncturing the head-neck part of the pig to be subjected to plasma collection by adopting a puncture needle connected with an injection syringe, pulling out the injection syringe when red blood appears in the injection syringe, inserting a guide wire into the puncture needle, and pulling out the puncture needle; and 4, slowly pushing a collecting catheter into the blood vessel along the guide wire, and pulling out the guide wire. With the method for collecting the plasma of the live pig, the plasma of the pig can be effectively and conveniently obtained, and does not need secondary separation, and therefore, the environmental pollution and the secondary pollution caused by the environment to the plasma are reduced; meanwhile, the initial bacterium contamination number of the plasma is reduced, the transmission of the zoonotic diseases is completely eradicated, the hemolysis phenomenon is avoided, and thus a reliable quality guarantee is provided for a biological product taking the plasma of the pig as the raw material.

The invention relates to a preparation method of a cortex phellodendri composite biological glass tissue repair material, and belongs to the field of biomedical materials, wherein the preparation method comprises: preparing a cortex phellodendri extraction liquid, preparing a polylactic acid solution, preparing a biological glass solution, and carrying out electrospinning to obtain the repair material. According to the present invention, the cortex phellodendri has the antibacterial effect, the polylactic acid can improve the mechanical property of the material, and the biological glass can improve the pharmaceutical property of the material; and the prepared tissue repair material has advantages of good mechanical property, good tissue repair property, and good drug adhesion property.

The invention relates to a method for producing fresh-cut fruits and vegetables through a high pressure carbon dioxide technology. The method comprises 1, selecting fresh fruits and vegetables and cutting the fruits and vegetables to obtain slices or blocks, 2, pre-cleaning the slices or blocks, putting the slices or blocks in an asepsis environment and carrying out natural drainage, wherein the cleaning solution comprises a ClO2 solution (with a concentration of 20-200ppm), electrolysed water (with ACC of 10-50ppm) and tap water, cleaning time is in a range of 10-15min and according to different raw material characteristics, an ultrasonic assisted cleaning technology can be adopted, and 3, carrying out room temperature seal sterilization treatment on the fruit and vegetable slices or blocks through a high pressure carbon dioxide technology to obtain fresh-cut fruits and vegetables, wherein high pressure carbon dioxide sterilization treatment pressure is in a range of 1-10MPa and treatment time is in a range of 10-20min. Under the storage condition of a temperature of 4 DEG C, the fresh-cut fruits and vegetables have a long shelf life, retrain nutrients, quality and functions and accelerate high pressure carbon dioxide technology marketization and commercialization progress.

The invention relates to the technical field of sludge treatment, and particularly discloses an in-site sludge improvement method and a sludge planting soil. The in-situ sludge improvement method comprises the following steps: S1, dewatering dredged sludge to enable the water content of the dewatered sludge to be less than or equal to 60%, and crushing the dewatered sludge into sludge particles; S2, uniformly mixing the sludge particles with a sludge modifier to obtain the sludge planting soil, wherein the sludge modifier comprises river sand, coco coir, branch particles, a pelelith filter material and a deodorant; and S3, using the sludge planting soil for landscape greening in site. The sludge in rivers and lakes is improved in situ, so that rapid treatment and reutilization of the sludge are realized, maximized and efficient reutilization of resources is realized, and the method has remarkable social benefits, environmental benefits and economic benefits.

The invention specifically provides a chicken Escherichia coli biological disinfectant. The chicken Escherichia coli bacteriophages Ecp2, Ecp3 and Ecp6 are adopted as raw materials, the chicken Escherichia coli biological disinfectant has a good disinfection effect on chicken colibacillosis outbreak henhouses, the disinfection effect of the chicken Escherichia coli biological disinfectant provided by the invention is better than that of didecyl ammonium bromide, the chicken Escherichia coli biological disinfectant is short in action time and fast in action, the total number of bacteria in a chicken coop can be obviously reduced, the bacteria amount is reduced to 0.30% of that of a control group after spraying disinfection is carried out for 30 minutes, the bacteria amount is only 0.10% of that of the control group after spraying disinfection is carried out for 70 minutes, and the killing effect on the total number of bacteria is more obvious than that of a didecyl ammonium bromide disinfectant. The disinfectant has good effects on developing green and pollution-free environment disinfectants and improving the economic benefits of broiler chicken breeding.

The invention discloses a microbial agent, comprising, by weight, 40 to 100 parts of Bacillus pumilus and 20 to 50 parts of mesohaline Rhodospirillaceae. The invention simultaneously discloses a preparation method for the microbial agent. In the invention, two microbial bacteria are employed as the raw materials, which is convenient for enterprises to accurately discriminate the two in an industrial production, thereby reducing cost. Since microbes have a short metabolism cycle and produce no pollution to the surroundings, the microbial agent provided in the invention is applicable to the industries of environmental protection, culturing and plantation.