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Chicken Escherichia coli biological disinfectant, and preparation and application thereof

A biological disinfectant and chicken Escherichia coli technology, which is applied in the direction of disinfectants, biocides, chemicals for biological control, etc., can solve the problem of unsatisfactory disinfection of chicken coops with chicken Escherichia coli disease and long disinfection time and other problems, to achieve significant disinfection effect, good disinfection effect, and excellent disinfection effect

Inactive Publication Date: 2021-08-06
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the existing phage preparations in the prior art, the disinfection of chicken coops with Escherichia coli disease is unsatisfactory, and the technical problem of long disinfection time, for this reason, the present invention uses pathogenic Escherichia coli lytic phages to form , to prepare chicken Escherichia coli biological disinfectant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment one: a kind of chicken escherichia coli biological disinfectant

[0030] The present invention specifically provides a biological disinfectant for chicken Escherichia coli, wherein the biological disinfectant for chicken Escherichia coli comprises coliphage Ecp2, Ecp3, and Ecp6.

[0031] The coliphage Ecp2, Ecp3, and Ecp6 are mixed in a volume ratio of 1:1:(3-1).

Embodiment 2

[0032] Embodiment two: a kind of preparation method of chicken escherichia coli biological disinfectant

[0033] The present invention specifically provides a kind of preparation method of chicken escherichia coli biological disinfectant, and concrete production steps are as follows:

[0034] Take out the three strains of phage isolated from Ecp2, Ecp3, and Ecp6 from the -80°C refrigerator, amplify and reproduce the corresponding host bacteria with liquid LB for 8-12 hours, take 1 ml of each of the bacteria liquid and phage, and add 5 ml of LB liquid culture medium In a test tube, shake culture at 37°C and 160r / min for 8-12 hours, and determine that the phage titer reaches 10 8 More than pfu / ml, that is, the proliferation of phage is completed, and mixed according to the volume ratio, that is, a chicken Escherichia coli biological disinfectant is obtained.

Embodiment 3

[0035] Embodiment three: a kind of chicken escherichia coli biological disinfectant

[0036] Take out the three strains of phage isolated from Ecp2, Ecp3, and Ecp6 from the -80°C refrigerator, amplify and reproduce the corresponding host bacteria with liquid LB for 8-12 hours, take 1 ml of each of the bacteria liquid and phage, and add 5 ml of LB liquid culture medium In a test tube, shake culture at 37°C and 160r / min for 8-12 hours, and determine that the phage titer reaches 10 8 More than pfu / ml, that is to complete the proliferation of phage, according to the volume ratio of 1:1:3 mixed, that is, the preparation of chicken Escherichia coli biological disinfectant.

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PUM

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Abstract

The invention specifically provides a chicken Escherichia coli biological disinfectant. The chicken Escherichia coli bacteriophages Ecp2, Ecp3 and Ecp6 are adopted as raw materials, the chicken Escherichia coli biological disinfectant has a good disinfection effect on chicken colibacillosis outbreak henhouses, the disinfection effect of the chicken Escherichia coli biological disinfectant provided by the invention is better than that of didecyl ammonium bromide, the chicken Escherichia coli biological disinfectant is short in action time and fast in action, the total number of bacteria in a chicken coop can be obviously reduced, the bacteria amount is reduced to 0.30% of that of a control group after spraying disinfection is carried out for 30 minutes, the bacteria amount is only 0.10% of that of the control group after spraying disinfection is carried out for 70 minutes, and the killing effect on the total number of bacteria is more obvious than that of a didecyl ammonium bromide disinfectant. The disinfectant has good effects on developing green and pollution-free environment disinfectants and improving the economic benefits of broiler chicken breeding.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, relates to the technical field of preparation of microbial preparations, in particular to the technical field of a chicken Escherichia coli biological disinfectant and its preparation and application. Background technique [0002] my country is the world's largest chicken-raising country, with an annual total of more than 4 billion chickens. The number of broiler chickens in Xinjiang is 40-50 million, and the number of laying hens is 7-8 million. Chicken colibacillosis ranks first among chicken bacterial diseases, accounting for about 33%. In my country, more than 31.07 million chickens die every year due to E. coli disease, and the economic loss is more than 300 million yuan. According to incomplete statistics, the direct or indirect economic losses caused by chicken colibacillosis account for 8%-11% of the feeding cost. Traditional drug prevention and control methods have faced challen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/40A01P3/00A01P1/00
CPCA01N63/40
Inventor 梁晏屈勇刚杨瑞钰常军帅杨慧敏刘功成吴圆圆
Owner SHIHEZI UNIVERSITY
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