Technology for fermenting and decomposing protein feed with protease in two steps by using probiotics
A protein feed and probiotic technology, applied in animal feed, animal feed, application and other directions, can solve the problems of large loss, poor decomposition effect and high cost
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Embodiment 1
[0012] Example 1: 90% soybean meal, 10% bran, add water to 45%, air-cool to 35°C after high-temperature sterilization, inoculate Aspergillus oryzae, insulate and ferment for 28 hours, start ventilation and cooling in 13 hours, and keep the fermentation process at 30-35°C ℃, immediately after fermentation, mix with soybean meal according to the ratio of dry matter 1:3, add 10% corn steep liquor and replenish water to 50% water content, stir evenly, compact and seal, ferment for 3 days, and immediately dry at low temperature after taking it out. , amino acid nitrogen content is 2.4%, organic acid is 3%.
Embodiment 2
[0013] Example 2: 80% cottonseed meal, 20% bran, add water to 50%, air-cool to 35°C after high-temperature sterilization, inoculate Aspergillus niger, heat preservation and fermentation for 32 hours, ventilate and cool down in 16 hours, and the fermentation process remains at 30-30°C. 35°C. Immediately after fermentation, mix with soybean meal according to the ratio of dry matter 1:4, add 10% corn steep liquor and replenish water to 47% water content, stir evenly, compact and seal, ferment for 4 days, and immediately dry at low temperature after taking out, After determination, the amino acid nitrogen content is 2.1%, and the organic acid content is 2.7%.
Embodiment 3
[0014] Embodiment 3: Feather meal 70%, bran 30%, add water to 50%, high temperature sterilization is cooled to 75 ℃, inoculate Bacillus subtilis, heat preservation and fermentation 25 hours, ventilate and cool down in 10 hours, the fermentation process remains at 33~38 ℃, immediately after fermentation, mix with high-temperature sterilization according to the ratio of dry matter 1:4, add 10% corn steep liquor and replenish water to 55% water content, stir evenly, compact and seal, ferment for 4 days, take it out and immediately low temperature After drying, the amino acid nitrogen content is 1.8%, and the organic acid is 2.3%.
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