Technology for fermenting and decomposing protein feed with protease in two steps by using probiotics

A protein feed and probiotic technology, applied in animal feed, animal feed, application and other directions, can solve the problems of large loss, poor decomposition effect and high cost

Inactive Publication Date: 2010-06-09
李军训
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patents have been applied for microbial aerobic fermentation of agricultural by-products, probiotic aerobic fermentation and lactic acid bacteria accumulation fermented feed, etc. These fermentation methods all have the disadvantage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: 90% soybean meal, 10% bran, add water to 45%, air-cool to 35°C after high-temperature sterilization, inoculate Aspergillus oryzae, insulate and ferment for 28 hours, start ventilation and cooling in 13 hours, and keep the fermentation process at 30-35°C ℃, immediately after fermentation, mix with soybean meal according to the ratio of dry matter 1:3, add 10% corn steep liquor and replenish water to 50% water content, stir evenly, compact and seal, ferment for 3 days, and immediately dry at low temperature after taking it out. , amino acid nitrogen content is 2.4%, organic acid is 3%.

Embodiment 2

[0013] Example 2: 80% cottonseed meal, 20% bran, add water to 50%, air-cool to 35°C after high-temperature sterilization, inoculate Aspergillus niger, heat preservation and fermentation for 32 hours, ventilate and cool down in 16 hours, and the fermentation process remains at 30-30°C. 35°C. Immediately after fermentation, mix with soybean meal according to the ratio of dry matter 1:4, add 10% corn steep liquor and replenish water to 47% water content, stir evenly, compact and seal, ferment for 4 days, and immediately dry at low temperature after taking out, After determination, the amino acid nitrogen content is 2.1%, and the organic acid content is 2.7%.

Embodiment 3

[0014] Embodiment 3: Feather meal 70%, bran 30%, add water to 50%, high temperature sterilization is cooled to 75 ℃, inoculate Bacillus subtilis, heat preservation and fermentation 25 hours, ventilate and cool down in 10 hours, the fermentation process remains at 33~38 ℃, immediately after fermentation, mix with high-temperature sterilization according to the ratio of dry matter 1:4, add 10% corn steep liquor and replenish water to 55% water content, stir evenly, compact and seal, ferment for 4 days, take it out and immediately low temperature After drying, the amino acid nitrogen content is 1.8%, and the organic acid is 2.3%.

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PUM

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Abstract

The invention relates to technology for fermenting and decomposing protein feed with protease in two steps by using probiotics, which is characterized in that the protein is fermented and decomposed with protease of aerobic fermentation matter in the anaerobic condition, soybean dregs, cotton dregs, vegetable dregs meal, wheat bran and other vegetable protein raw materials are sterilized at high temperature and then are aerobically fermented for 24-36h, feather meal and other animal protein raw materials are aerobically fermented for 20-26h, the inoculating temperature is controlled at 60-80 DEG C, the fermented materials are not dried and are directly mixed with fresh raw materials, corn steep liquor and other nutrient solution are added or not added; lactic acid bacillus are inoculated to the mixture, and water is supplemented to the mixture until the water content of the mixture reaches 40-60 percent, the mixture is compacted and sealed to be anaerobically fermented for 1-3d above 20 DEG C or 2-5d below 20 DEG C, and the mixture is dried after being fermented and decomposed with protease.

Description

technical field [0001] The invention relates to a probiotic two-step fermented protein feed technology, which belongs to the technical field of probiotic solid-state fermented protein feed. Background technique [0002] In 2008, the total output of commercial feed in my country reached 138 million tons, and a large amount of protein feed is needed every year. However, protein feed is a relatively scarce raw material in my country. The annual import of foreign protein feed is nearly 40 million tons, which has formed a situation where humans and animals compete for food, protein raw materials. On the other hand, my country still uses the American corn-soybean-fishmeal feed formula mode, and many rich resources such as by-products of agricultural product processing are not well utilized, such as cotton meal, rapeseed meal, distiller's grains, feather meal, etc. because of anti-nutritional factors or Poisonous ingredients cannot be well utilized. Although the utilization rate of...

Claims

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Application Information

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IPC IPC(8): A23K1/14
Inventor 李军训高洁
Owner 李军训
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