47results about How to "Less bacteria" patented technology

The invention relates to technology for fermenting and decomposing protein feed with protease in two steps by using probiotics, which is characterized in that the protein is fermented and decomposed with protease of aerobic fermentation matter in the anaerobic condition, soybean dregs, cotton dregs, vegetable dregs meal, wheat bran and other vegetable protein raw materials are sterilized at high temperature and then are aerobically fermented for 24-36h, feather meal and other animal protein raw materials are aerobically fermented for 20-26h, the inoculating temperature is controlled at 60-80 DEG C, the fermented materials are not dried and are directly mixed with fresh raw materials, corn steep liquor and other nutrient solution are added or not added; lactic acid bacillus are inoculated to the mixture, and water is supplemented to the mixture until the water content of the mixture reaches 40-60 percent, the mixture is compacted and sealed to be anaerobically fermented for 1-3d above 20 DEG C or 2-5d below 20 DEG C, and the mixture is dried after being fermented and decomposed with protease.

The invention belongs to the technical field of plant tissue culture and particularly relates to a method for building a bamboo reed tissue culture system. The method comprises the steps of (1) explant disinfection, wherein axillary buds of bamboo reeds are fully soaked in 75% of ethanol for 10 seconds and then moved into 0.2% of mercury bichloride solution for 10 minutes; (2) primary culture, wherein a culture medium is formed by adding cane sugar 30g / L and agar 8g / L in MS, then adding 6-BA 5mg / L and IBA 0.5mg / L and finally adjusting potential of hydrogen (pH) to 5.8; (3) secondary culture, wherein a culture medium and culture conditions of the secondary culture are the same as the culture medium and culture conditions for the primary culture; (4) strong bud and root culture, wherein the buds are cultured in the secondary culture medium for one month to finish strong bud culture and then planted in a rooting culture medium, and the rooting culture medium is formed by adding IBA 0.5 mg / L into the MS; and (5) domestication and transplantation, wherein the domestication is performed by using a floating bed domestication system for one week and then transplantation is performed. By means of the method, the efficient breeding of the bamboo reeds can be achieved.

The invention provides a method for production of ethanol by continuous fermentation of immobilized yeast cells, and the method comprises the following steps: (1) a fiber material is attached to a support frame, and is filled into a bioreactor; (2) yeast cells are immobilized in the fiber material in the bioreactor; (3) a fermentation medium is added into the bioreactor to begin ethanol fermentation; (4) when glucose in the fermentation medium is consumed, a fermentation liquid flows out of the bioreactor, the ethanol is collected, the fermentation medium is added, and then the step (4) is repeated. The method uses the fiber material for immobilization of the yeast cells for continuous fermentation to produce the ethanol, the fiber material as an immobilization material is stably in property, non-toxic, and good in adsorption and mass transfer effects, can be repeatedly used, is easy to achieve continuous production of the ethanol, and in a continuous fermentation process can greatly improve the ethanol yield.

The invention discloses an integration processing method of a high-efficient corn straw biological feed, and belongs to the field of biological feeds. Corn straw is subjected to three-step fermentation, so that a superior microorganism coarse feed for ruminant animals, with high efficiency and low cost, is produced in a large-scale manner. The integration processing method comprises the following steps of firstly, based on the corn straw, performing high-efficient pure solid cultivation on a white rot fungus enzyme preparation, then converting the cultivated white rot fungus enzyme preparation in large inoculum amount to a first-stage fermentation medium of the corn straw, and besides, inoculating serial lignocelluloses degradation bacteria and candida utilis bacteria for synergistic rapid degradation and transformation; then adding bacillus and saccharomyces cerevisiae for second-stage fermentation; and using lactic acid bacteria for third-stage anaerobic fermentation so as to obtain finished products. The raw materials used by the integration processing method do not need to be sterilized, the finished products do not need to be dried, lysozymes are enriched, the cost is low, the lignin is notably reduced, and genuine protein is increased to 12% or above. The high-efficient corn straw biological feed can be used as a superior coarse feed and a protein grain feed for cattle and sheep, and the additive quantity can reach 60% or above; the additive quantity can also be about 10%, so that the high-efficient corn straw biological feed is used for simple stomach animals. The technique is high in scalability and integrity, and the economic and social benefits are notable.

The invention belongs to the technical field of microbial fermentation, and provides a micro-ecological compound microbial agent and a method for processing a solid crop culture medium through the micro-ecological compound microbial agent for solving the problems that an existing culture medium cannot be reused, and if the culture medium is reused, continuous cropping obstacles are prone to being caused, and the culture medium becomes a carrier of soil-borne disease pollution and concentrated spread. The micro-ecological compound microbial agent is prepared by mixing bacillus amyloliquefaciens LH-1, bacillus licheniformis, pseudomonas cichorii, propionigenium modestum, thermotoga aritime, bacillus stearothermophilus, candida tropicalis, trichoderma harzianum, streptomycete, actinomyces meyeri and frankiaceae according to a certain ratio. According to the micro-ecological compound microbial agent and the method for processing the solid crop culture medium through the micro-ecological compound microbial agent, pathogenic bacteria can be effectively killed, and the continuous cropping obstacles are overcome; the mode that biological and physical measures are combined is scientifically applied to solve the problems existing in reuse of the matrix, the fruit and vegetable quality safety and zero pollution to the environment are guaranteed, the production cost of farmers is lowered, the agricultural product quality safety is guaranteed, and the ecological environment is protected; the culture medium can be reused for many times, and the growth and development of crops cannot be influenced.

The invention relates to a tissue culture method for rapidly propagating wild lilies and belongs to the field of a flower culture technology. According to the method, bud-stage flower stems of the lilies are used as explants of tissue culture of the lilies and are completely disinfected before culture; besides that 6-BA (Benayl Aminopurine), NAA (Naphthalene Acetic Acid) and IBA (Indole Butyric Acid) are used as auxins or hormones in a culture medium, an MES (Methyl Ester Sulfonate) is added to be used as a buffering agent so as to effectively control the pH value in a tissue culture process, and the pH value is maintained in the optimal range of differentiation, propagation and growth of the lily explants; therefore, the induction efficiency and the propagation coefficient are improved, the induction rate is 98%-100%, the quantity of adventitious buds induced by each explant is not less than 15, the propagation coefficient is 5-7, the rooting rate is 100%, and the root system is healthy and strong. The tissue culture method is applicable to safe propagation of good single plants of filial generations and is an effective manner for protecting wild resources of lilies.

A preparation method of an oudemansiella raphanipes liquid strain comprises the steps that firstly, a culture medium is prepared, wherein 1 kg of the culture medium serves as a benchmark, the culturemedium contains 300-400g of potato juice, 100-200 g of grape juice, 30-50 g of agar, 0.5-1 g of magnesium sulfate, 3-6 g of paper mulberry fermented powder and balance water; secondly, the culture medium is sterilized, wherein after being filtered, the culture medium obtained in the first step is placed in a sterilization device to be sterilized, and then is cooled after sterilization; thirdly, astrain is inoculated, wherein oudemansiella raphanipes mycelia are inoculated in the culture medium obtained in the second step, and then the culture medium is placed on a shaking bed for oscillationculture; fourthly, fermentation culture is conducted, wherein the liquid strain obtained after oscillation culture in the third step is placed in a fermentation tank for fermentation culture. According to the method, the liquid culture medium prepared from the potato juice, the grape juice, the agar, the magnesium sulfate and the paper mulberry fermented powder is used, the components work together to provide various nutrients for growth of the mycelia, the growth speed of the mycelia can be high, the biomass of the mycelia is 2.3 g / ml or above, and the mycelia are thick and a little amber.

The present invention is directed to a magnetic drinking cup, which is a drinking cup containing magnetic and metal components. The magnetic and metal components allow for a tight seal and decrease the opportunity for bacteria and mold to flourish. Many cups known in the prior art have screw top lids, which have tight and narrow crevices making the cleaning process cumbersome. If not cleaned immediately after use, it becomes challenging to remove the debris remaining in the cup, and around the inside of the lid. These small areas create a trap for bacteria and mold, if not cleaned properly. The same issues can arise with reusable straws. There are not many tools on the market that can clean a straw thoroughly from top to bottom. If not all contents are removed from the straw, it becomes a petri dish of bacteria, and if moisture remains, then mold will have the ability to grow. This invention allows for cleaning ease, and provides fewer areas where bacteria and mold can flourish due to a magnetic seal instead of a screw top seal. Further, the drinking cup can be replaced with any storage container, including children's “sippy cups”, Tupperware, or any food storage container that requires a tight seal.

The invention discloses a method for using organic tin-degrading bacteria agent and remediation plants to treat organic tin polluted water. According to the invention, a large amount of organic tin-degrading bacteria are obtained through fermentation technology, and the bacteria are dried to obtain active bacteria agent which can be stored for a long time. Under the condition of planting remediation plants, the organic tin-degrading bacteria are added to remove organic tin by using the combined processing technology of organic tin-degrading bacteria agent and remediation plants. The method has the advantages that the combined organic tin pollutant processing technology of organic tin-degrading bacteria agent and remediation plants is adopted, the treatment efficiency is high, the cost is low, the method does not cause secondary pollution, the technical effect of the method is better than single microbial purifying, the quantity of the organic tin-degrading bacteria required to be added into water is small, and the remediation plants also has the feature of landscaping.

The invention provides a method for producing an edible fungi stock and a cultivated species by fungus rod fungi culture, which is high-efficiency large-scale edible fungus production technology capable of reducing the period of culture bag fungus culture. After a culture medium is filled into a culture bag by a bag filling machine or hands at a proper looseness, a more than 10 centimeter cone-shape fungus rod is inserted into the culture medium from the upper surface of the culture medium; the head of the fungus rod is flush with a culture material surface; and the head of the rod is fastened with a rope fastener. For inoculation in fungus bags, the fungus rod is pulled out, a fungus species is directly inoculated into the centers of the fungus bags under the condition of an inoculation amount no higher than that of surface solid inoculation, and the fungi grow toward four sides from the centers of the fungus bags. The spawn running time is reduced greatly, the survival rate is high, and both the spawn running and fungus age in the fungus bags are consistent. In a black fungus bag at a proper temperature, hyphae grow in the whole fungus bag after 25 to 30 days. The fungus rod fungi culture is a 'solidified liquid species' fungus culture technology advantageous over liquid species, requires a smaller fungus culture investment and can obviously improve economic benefits for cultivators.

The invention discloses a method for producing baohuoside I through herba epimdii. The method comprises the following steps of: S1. expanding propagating and diluting saccharomyces cerevisiae in culture medium; S2. mixing and evenly stirring culture solution and herba epimdii, and carrying out close and static anaerobic fermentation in a fermentation tank; S3. when the herba epimdii is entirely hydrolyzed, extracting fermenting raw materials with an extraction solvent; S4. washing with extract dichloromethane, and extracting ethyl acetate; and S5. filtering ethyl acetate through an alumina column, then carrying out silica gel adsorption column chromatography to obtain pure baohuoside I. The method has the beneficial effects of being simple and convenient to operate, easy to implement, high in efficiency, fast, low in cost, high in yield, pollution-free by adopting microbial solid-state anaerobic fermentation to hydrolyze herba epimdii-containing raw materials, thus being suitable for large-scale industrial production.

The invention relates to a method of preparing a fermented feed with grape residues as a main raw material through multi-stage fermentation. The feed not only basically satisfies protein demand of animals fed thereon, but also has health-caring effects of improving intestinal tract health and enhancing immunity of the animals. With the grape residues as a raw material, the feed is low in cost and has wide raw material source, achieves comprehensive utilization of the grape residues, and has excellent ecologic and social benefits.

The invention relates to a method for preparing stinky mandarin fish by adding minced fillet to assist fermentation. The method comprises the following steps: (1) high-frequency vibration unfreezing;(2) ultrasonic-assisted brine sterilization and cleaning; (3) minced fillet pretreatment; (4) pickling liquid preparing; (5) constructing a pickling system; (6) performing low-temperature fermentation. According to the invention, minced fillet is added for auxiliary fermentation to prepare the stinky mandarin fish, so that the fermentation time is shortened, and the sensory quality of the stinkymandarin fish is improved.

The invention relates to a method for quickly identifying bacteria and fungi by using Raman spectra, which comprises the following steps: pretreating a sample to be identified, collecting the Raman spectra of the treated sample, removing the background of the collected Raman spectra and performing normalizing treatment, and identifying bacteria and fungi by using the treated spectra. Compared withthe prior art, the method is simple to operate, and complex sample pretreatment is not needed before Raman testing. According to the method, the amount of bacteria required for testing is small, enrichment culture is not needed, the detection time can be greatly shortened, and an identification result can be quickly given. By utilizing the characteristic that Raman spectra of cells can reflect chemical components of the cells, bacteria and fungi are accurately identified by comparing specific peak positions of the Raman spectra of the bacteria and the fungi, and errors caused by observing themorphological sizes of the cells by naked eyes and subjectively judging can be eliminated.

The invention relates to a citrus reticulata peel residue feed preparation method, wherein the main raw material is citrus reticulata peel residue, and multi-stage fermentation is performed to prepare the feed. According to the present invention, the prepared feed can basically meet the protein requirement of animal feeding, and contains a certain amount of oligosaccharides; after the prepared citrus reticulata peel residue feed is added to feeds, the palatability is good, the animal feed intake is increased, and the health effects of animal intestinal tract health improving and animal immunity improving are achieved; and the equipment for production is less, the operation process is simple, the use effect is good, the citrus reticulata peel residue utilization problem can be fundamentally solved, the preparation method is one of the optimal schemes for the development and utilization of the citrus reticulata peel residue, the comprehensive utilization of the citrus reticulata peel residue is achieved, and the significant ecological and social benefits are provided.

The invention belongs to the technical field of beds special for patient nursing, and discloses a rehabilitation bed for burn. The rehabilitation bed comprises a bed frame and a bed board arranged onthe bed frame, a protective cover is further arranged on the bed board, the protective cover comprises two frame bodies located on the two sides of the bed board and protective films erected on the frame bodies, each frame body comprises a fixed rod and a plurality of sliding rods transversely connected to the bed board in the length direction of the bed board in a sliding mode, and a foldable connecting piece is arranged between every two adjacent sliding rods; a connecting rod is fixed between the sliding rods, far from the fixed rod, of the two frame bodies; and a gas replacement system isalso arranged on the bed board. The invention solves the problems that at present, due to the fact that a sterile ward is short, a burnt patient can only recover in a common ward, the affected part can make contact with dust and bacteria in air, and infection is likely to be caused.

The invention relates to a tissue separation medium for wild domestic fungus strain and a preparation method. A base medium is prepared according to the following formula: in parts by mass, 20-30 parts of glucose, 5-7 parts of peptone, 2-5 parts of potassium dihydrogen phosphate, 1-3 parts of magnesium sulfate, 15-20 parts of agar powder, and 1000 parts of water; the base medium also comprises 0.05%-0.20% of an antifungal medicine according to the mass percent concentration; and in the base medium which is added with the antifungal medicine and is subjected to sterilization and cooling, per one liter of the medium also comprises 500 units-10000 units of two antibiotics. After the base medium is stirred uniformly, the base medium is subpackaged into plastic test tubes with covers under aseptic condition when the base medium is hot, and the subpackaged base medium is cooled for usage. After being sent to a laboratory, a wild strain resource material acquired by employing the medium disclosed by the invention is strong in vitality, less in infectious microbe and easy to purify, the medium helps to provide a reliable effective technological means for obtaining wild domestic fungus resource, and has extremely high application value.

The invention relates to a method of preparing a feed from the stem and leaf of pepper. According to the method, the main raw materials are stems and leaves of pepper, and the feed is prepared by fermenting the raw materials in multiple stages. The prepared feed can basically fulfill the needs of animals on proteins, and at the same time, is capable of improving the health state of intestines of animals and strengthening the immunity of animals. The raw materials are stems and leaves of pepper, and thus are cheap and easily available, and the comprehensive utilization of stems and leaves of pepper is achieved.

The invention provides a preparation process of a novel magnetized three-stage fermentation organic fertilizer. The preparation process comprises a fermentation process, a decomposition and aging process, a microwave treatment process, a magnetization treatment process and material compounding. According to the preparation process, three stages of processes of decomposing and aging, microwave treatment and magnetization treatment are added for the first time on the basis of traditional aerobic fermentation, so that the treated material is richer in micromolecular organic nutrition, less in infectious microbes and harmful substances and more stable in fertilizer efficiency.

The invention discloses a seedling raising method of an eastern white pine, which comprises the following steps of: carrying out seedling raising of the eastern white pine in winter by utilizing a greenhouse, prolonging the growth period of the eastern white pine, avoiding low-temperature freezing damage in the seedling period and improving the quality of seedlings; using quartz sand instead of river sand during seed lamination, which is beneficial to improving the working efficiency during dibbling; during seeding, using a degradable non-woven fabric nutrition pot instead of a seedling tray,a plastic nutrition pot or open field seeding, so that the bed changing or transplanting times can be effectively reduced, and the seedling stage does not exist; in the cultivation medium, using decomposed sheep manure instead of other decomposed organic fertilizers, so that the pH value of the soil can be improved, and the soil fertility can be improved; using peat soil instead of garden soil, sothat the organic matter content in the matrix is effectively improved, and the stability of the pH value of the soil is maintained; after the dibbling is finished, spreading a layer of 0.3-0.6cm sawdust, so that the seeds can be prevented from floating upwards during watering. The method improves the breeding quantity and quality of the seedlings and saves the cost.

The invention belongs to the technical field of microorganisms and particularly discloses a special culture medium for isolating endophytic trichoderma fungi as well as a preparation method and application thereof. The special culture medium comprises the following components: potatoes, glucose, agar powder, chloramphenicol, streptomycin sulfate, rose-bengal, quintozene and water; the potatoes, the glucose, the agar powder and the water are prepared into a PDA culture medium; in the PDA culture medium per liter, the content of the chloramphenicol is 50-150 mg, the content of the streptomycin sulfate is 4-8 mg, the content of the rose-bengal is 25-100 mg, and the content of the quintozene is 6-10 mg. The special culture medium disclosed by the invention can efficiently, quickly and accurately isolate the endophytic trichoderma fungi, is low in trichoderma fungi inhibition rate and good in inhibiting effects on other miscellaneous bacteria, can form the relatively-single trichoderma fungi with better colony characteristics and is more in isolated species, easy to identify and purify and strong in specificity.

The invention relates to the technical field of pickle production, and discloses lactic acid bacteria pickles and a preparation method thereof. The preparation method comprises the following steps: S1, sequentially adding lactic acid bacteria fermentation liquor A and vegetable raw materials into a pickle jar; S2, placing the pickle jar in the step S1 at 28-35 DEG C, and performing fermenting for 1-2 days; S3, placing the pickle jar fermented in S2 at 0-8 DEG C, and performing fermenting for 3-5 days; S4, adding lactic acid bacteria fermentation liquor B into the pickle jar fermented in S3, and perfomring fermenting at 15-25 DEG C for 5-10 days; and S5, taking out the pickles in the pickle jar in the step S4, and performing bagging and sealing. The lactic acid bacteria fermentation liquor A contains garlic juice, ginger juice and a lactic acid bacteria agent, and the lactic acid bacteria fermentation liquor B contains garlic juice and ginger juice. The lactic acid bacteria pickles have the advantages of being high in lactic acid bacteria activity and short in fermentation time.

The present invention is directed to a magnetic drinking cup, containing magnetic and metal components. Many cups known in the prior art have screw top lids, which have tight and narrow crevices making the cleaning process cumbersome. If not cleaned immediately after use, it becomes challenging to remove the debris remaining in the cup, and in the crevices of the lid. These crevices create a trap for bacteria and mold. The same issues can arise with reusable straws. There are not many tools on the market that can clean a straw thoroughly from top to bottom. If all contents are not removed from the straw, then it becomes a petri dish of bacteria; and if moisture remains, then mold will have the ability to grow. This invention provides fewer areas where bacteria and mold can flourish due to a magnetic seal instead of a screw top seal.

The invention discloses a cultivation method of needle mushrooms and relates to the technical field of cultivation of edible mushrooms. The method comprises the following steps of preparation of a culture medium, bagging and sterilization, inoculation, management of a mushroom growing period, management of a fruiting period and harvesting. According to the cultivation method, by selecting the culture medium and regulating and controlling the temperature, air humidity and other factors of the mushroom growing period and the fruiting period, the yield of the needle mushrooms is further increased, the quality of the needle mushrooms is further improved, the production period is shortened, hyphae of the cultivated needle mushrooms are dense, thick, strong and even, fruiting is orderly, the taste is fragrant, the yield and quality are high, and the economic benefit is improved.

The invention belongs to the technical field of macadamia ternifolia F.Muell. cultivation, in particular to a method for promoting callus induction of macadamia ternifolia F.Muell. stems. The method comprises the five steps of explant collection, explant pretreatment, disinfection, inoculation and culture. By means of a treatment scheme of the invention, the explant contamination rate is 5% or below; the callus induction rate is 96% or above; calluses and regenerated buds can be obtained in a short period of time; the calluses can be used as inducing materials for sterile buds; no disinfectiontreatment is needed; the regenerated buds grow faster; and a situation of cell tissue necrosis is not prone to occur.

The invention discloses a method for using organic tin-degrading bacteria agent and remediation plants to treat organic tin polluted water. According to the invention, a large amount of organic tin-degrading bacteria are obtained through fermentation technology, and the bacteria are dried to obtain active bacteria agent which can be stored for a long time. Under the condition of planting remediation plants, the organic tin-degrading bacteria are added to remove organic tin by using the combined processing technology of organic tin-degrading bacteria agent and remediation plants. The method has the advantages that the combined organic tin pollutant processing technology of organic tin-degrading bacteria agent and remediation plants is adopted, the treatment efficiency is high, the cost is low, the method does not cause secondary pollution, the technical effect of the method is better than single microbial purifying, the quantity of the organic tin-degrading bacteria required to be added into water is small, and the remediation plants also has the feature of landscaping.

The invention provides a method for production of ethanol by continuous fermentation of immobilized yeast cells, and the method comprises the following steps: (1) a fiber material is attached to a support frame, and is filled into a bioreactor; (2) yeast cells are immobilized in the fiber material in the bioreactor; (3) a fermentation medium is added into the bioreactor to begin ethanol fermentation; (4) when glucose in the fermentation medium is consumed, a fermentation liquid flows out of the bioreactor, the ethanol is collected, the fermentation medium is added, and then the step (4) is repeated. The method uses the fiber material for immobilization of the yeast cells for continuous fermentation to produce the ethanol, the fiber material as an immobilization material is stably in property, non-toxic, and good in adsorption and mass transfer effects, can be repeatedly used, is easy to achieve continuous production of the ethanol, and in a continuous fermentation process can greatly improve the ethanol yield.

The invention relates to the technical field of vegetable planting, in particular to a culture medium for soilless culture, which comprises the following components in parts by weight: 10-20 parts ofriver sand, 20-30 parts of plant ash, 1-5 parts of gabbro, 10-20 parts of Wali wormcast, 8-12 parts of mushroom residues, 1-2 parts of brewer's grain and 1-2 parts of bark powder. The pH value of theculture medium is 6.5-6.8, and the volume weight of the culture medium is less than 0.7g / cm<3>; According to the culture medium for soilless culture of vegetables, the Wali wormcast is added and has rich humic acid and a large number of beneficial microbial bacteria, 18 amino acids and various trace elements, and nutrients needed by plant growth can be provided. Beneficial active bacteria are contained, so that the fertilizer efficiency is slowly released and the fertilizer is continuously supplied. Wormcast is an organic matrix, the cation replacement capacity is high, and the buffering capacity, namely the acid-base property change alleviating capacity, of wormcast is high.

The invention discloses a method for inducing and multiplying adventitious buds of Cyclocarya paliurus, belonging to the technical field of plant tissue culture. Select the young shoots of Cyclocarya paliurus as explants, before the explants are induced and value-added culture, the explants are first sterilized and disinfected; the sterilized treatment adopts alcohol treatment for 25-30s, sodium hypochlorite treatment for 3-4min; Adventitious bud induction medium formula: WPM+6‑BA+sucrose+agar powder, and adjust pH to 5.75‑5.85; Cyclocarya adventitious bud proliferation medium formula: WPM+IBA+sucrose+agar powder. The present invention obtains a large amount of adventitious bud materials with strong growth, stretched leaves and high growth in a relatively short period of time by optimizing materials, sterilizing treatment and cultivating formula, laying the foundation for establishing an efficient in vitro rapid propagation system of Cyclocarya paliurus, and also It is of great significance to the protection, development and utilization of Cyclocarya paliurus germplasm resources.

The invention relates to the technical field of cultivation of macadimia nut, in particular to an adventitious bud induction method of macadimia nut. The method comprises the steps of using the macadimia nut as a tested material, carrying out hydroponic culture on newly sprouted tender branches used as explants, then washing the explants with water, transferring into an ultra-clean work table, soaking in Tween-20, disinfecting with alcohol, soaking in mercuric chloride, and washing with sterile water; after that, placing on sterilized filter paper for absorbing the surface water for later use;then, inoculating in an induction medium for carrying out lighting culture, and then inoculating a proliferation culture medium with the induced adventitious buds for carrying out proliferation culture. The method is used for carrying out macadimia nut adventitious bud induction under the in vitro condition, thus not only simplifying the disinfection step, but also obtaining vigorous and sterileadventitious buds.