Method for producing baohuoside I through herba epimdii

A technology of epimedium and baohuoside, which is applied in the field of extraction of traditional Chinese medicine components, can solve the problems of product damage, tedious and difficult control, high energy consumption of concentration, etc., and achieve the effects of reducing product loss, easy separation and purification, and simple process operation

Active Publication Date: 2013-09-18
XICHANG DANYANG BIOLOGY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] 1. Due to low-alcohol extraction, the extract contains a lot of water, high energy consumption for concentration, high concentration temperature and long time
Since the C-8 position of baohuoside I has a sensitive isopentenyl group, and other parts have relatively sensitive carbonyl and ketone groups, high temperature for a long time will greatly damage the product
At the same time, low-alcohol contains more water and brings out more impurities, which increases the difficulty of separation
The water liquid is left after the concentration of low-alcohol, which does not dissolve the target product baohuoside I, which affects the extraction effect
The addition of emulsifier Tween 80 only helps the proposed icariin to be hydrolyzed into baohuoside I and partly dissolves into water, while baohuoside I in plant tissues is difficult to extract, resulting in incomplete presentation
[0010] 2. The price of cellulase is high, and 5 kg per ton of raw materials is used, which is a large amount
Make this process product without cost advantage
4. Due to the above defects of the process (unclean extraction, many impurities brought out, and large product damage), the content of the extract is low, resulting in difficulty in product purification
It is cumbersome and difficult to control, and the cost is too high

Method used

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  • Method for producing baohuoside I through herba epimdii
  • Method for producing baohuoside I through herba epimdii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The method for producing baohuoside I by fermentation of Epimedium comprises the following steps:

[0060] S1, take 40g of highly active dry yeast and propagate in the medium for 10 hours, the propagation culture conditions are pH6~7, temperature 25 ℃, static fermentation, then add 2 times the amount of water according to the volume of the propagation solution to dilute to obtain a culture solution; Described culture medium is made up with starch and water, the consumption of starch is 500 times (20kg) of yeast weight, the water to starch weight ratio added in starch is 6:1, and described starch is wheat flour; high activity dry yeast It is "highly active dry yeast" stipulated by the industry standard of the People's Republic of China QR / T1501-92, excellent or first-class; fermentation capacity ≥ 750ml (CO 2 ).

[0061] S2, mixing the culture solution obtained in step S1 with 240kg pulverized to 10~20 mesh Epimedium dry raw materials, detecting the icariin content to ...

Embodiment 2

[0072] The method for producing baohuoside I by fermentation of Epimedium comprises the following steps:

[0073] S1, take 300g of highly active dry yeast and propagate in the medium for 8 hours, the propagation culture conditions are pH6~7, temperature 35 ℃, static fermentation, then add 2.5 times the volume of the propagation solution to dilute with water to obtain a culture solution; Described culture medium is made up with starch and water, the consumption of starch is 666.7 times (200kg) of yeast weight, the water and starch weight ratio added in starch is 5:1, and described starch is wheat flour; high activity dry yeast It is "highly active dry yeast" stipulated by the industry standard of the People's Republic of China QR / T1501-92, excellent or first-class; fermentation capacity ≥ 750ml (CO 2 ).

[0074] S2, the culture solution obtained in step S1 is mixed with 2.4 tons of dried Epimedium raw materials that are pulverized to 10-20 meshes, and stirred evenly. The fer...

Embodiment 3

[0084] The method for producing baohuoside I by fermentation of Epimedium comprises the following steps:

[0085] S1, take 40g of highly active dry yeast and propagate in the medium for 12 hours, the propagation culture conditions are pH6~7, temperature 15 ℃, static fermentation, then add 2 times the amount of water to obtain the culture solution according to the volume of the propagation solution; Described culture medium is made up of starch and water, the consumption of starch is 800 times of yeast weight, the weight ratio of water and starch added in starch is 4:1, described starch is wheat flour; high activity dry yeast is Chinese people "Highly active dry yeast" stipulated by the Republic of China's industry standard QR / T1501-92, excellent or first-class; fermentation capacity ≥ 750ml (CO 2 ).

[0086] S2, mixing the culture solution obtained in step S1 with 1000kg crushed to 10~20 meshes of fresh Epimedium, stir evenly, the weight ratio of fresh Epimedium to culture s...

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Abstract

The invention discloses a method for producing baohuoside I through herba epimdii. The method comprises the following steps of: S1. expanding propagating and diluting saccharomyces cerevisiae in culture medium; S2. mixing and evenly stirring culture solution and herba epimdii, and carrying out close and static anaerobic fermentation in a fermentation tank; S3. when the herba epimdii is entirely hydrolyzed, extracting fermenting raw materials with an extraction solvent; S4. washing with extract dichloromethane, and extracting ethyl acetate; and S5. filtering ethyl acetate through an alumina column, then carrying out silica gel adsorption column chromatography to obtain pure baohuoside I. The method has the beneficial effects of being simple and convenient to operate, easy to implement, high in efficiency, fast, low in cost, high in yield, pollution-free by adopting microbial solid-state anaerobic fermentation to hydrolyze herba epimdii-containing raw materials, thus being suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to an extraction process of traditional Chinese medicine ingredients, in particular to a method for producing baohuoside I by fermentation of Epimedium. Background technique [0002] Epimedium is the berberi plant Epimedium heartleaf, Epimedium brevicornum Maxim., Epimedium sagittatum (Sieb.et Zucc.) Maxim., Epimedium pilose (Epimedium brevicornum Maxim.) Epimedium pubescens Maxim.), Epimedium wushanense T.S. Ying, or the dry aerial parts of Epimedium koreanum Nakai. Harvest when the stems and leaves are lush in summer and autumn, remove thick stems and impurities, and dry in the sun or shade. [0003] Epimedium, a traditional Chinese medicine for nourishing, has various effects such as invigorating kidney yang, strengthening muscles and bones, and dispelling rheumatism. [0004] Baohuoside I (Baohuoside I), also known as Icarisid II (Icarisid II), exists in small amounts in Wujiapi and Epimedium pilosa. Baohuoside I has strong...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/60C12R1/865
Inventor 蒋洪武杨红刘卓艳波拉提.马卡比力江发玉
Owner XICHANG DANYANG BIOLOGY TECH CO LTD
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