18 results about "Epimedium sagittatum" patented technology

The invention discloses a flavonoid enantiomer compound extracted from Epimedium sagittatum and its preparation method and application. The preparation method comprises the following steps: crushing Epimedium sagittatum to prepare a paste, suspending the paste with water, extracting the paste with petroleum ether, dichloromethane, ethyl acetate and n-butanol respectively to obtain extracts of each part, performing gradient elution separation by silica gel column chromatography, combining similar parts according to the color development results of thin layer chromatography (TLC) to obtain multiple polar segments; chromatographing the target polar segment and performing gradient elution to obtain multiple components, and then combining similar components by thin layer chromatography (TLC) spot plate absorption to obtain multiple sub-components; eluting and purifying the target sub-components, collecting fractions with different retention times, and drying to obtain four compounds respectively. The four compounds have a significant inhibitory effect on the cell viability of human breast cancer MCF-7 cells and have anti-breast cancer activity. The four compounds can be used in the preparation of drugs for treating breast cancer, with huge social and economic benefits.

Isoprenyl flavonoid compounds extracted from Epimedium sagittatum and preparation method and application thereof, the preparation method is: crushing Epimedium sagittatum to paste, suspending with water, extracting with petroleum ether, dichloromethane, ethyl acetate and n-butanol respectively to obtain various parts of extract, using silica gel column chromatography to separate by gradient elution, according to the TLC coloration result, similar parts are combined to obtain multiple polarity sections; chromatograph the target polarity section, gradient elution, obtain multiple components, then combine similar components by TLC point plate absorption to obtain multiple component groups; elute and purify the target component group, collect the flow fraction with different retention time, dry, and obtain two compounds respectively, which have significant inhibitory effect on the cell viability of human breast cancer cell MCF-7, have anti-breast cancer activity, and can be applied to prepare breast cancer treatment drugs, with huge social and economic benefits.

The present invention discloses a methylotrophic Bacillus SY-22 and its application. This bacterium was registered and preserved in the General Microbiology Center of the China Committee for Culture Collection of Microorganisms on August 8, 2022, with the preservation number CGMCC No. 25501 and the preservation address at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Methylotrophic Bacillus SY-22 has good effects in preventing and / or treating Anthracnose of Epimedium sagittatum, and its disease prevention effect is much higher than that of chemical pesticides such as carbendazim and prochloraz-manganese chloride complex. It is a green and pollution-free biocontrol bacterium that can reduce the use of chemical pesticides.

The invention discloses a preparation method of an epimedium sagittatum extract. The preparation method comprises the steps of blanching, back washing, enzyme treatment, ethanol extraction, duplex filtration, column purification and concentration drying. According to the method disclosed by the invention, food-grade sodium carbonate used during blanching is safe and non-toxic, and a waxy coat on the surface of epimedium sagittatum is dissolved by a method of combining blanching and enzyme treatment, so that the problem of blockage of a subsequent duplex filter is solved, and the extraction rate of icariin in the epimedium extract is also improved.

The application discloses a kind of whole-process tracking management methods and systems for Epimedium sagittatum plantation, it is related to agricultural product management technical field, comprising: according to planting plot and planting time, generating planting batch number, constructing basic traceability file, based on agricultural key control point, obtain planting monitoring data, and association binding, set threshold to growth stage plant representation data, compare set threshold and planting node process file abnormal node, integrate planting node process file and abnormal disposal file, generate harvesting suitability score to determine planting batch, according to executable batch harvesting confirmation voucher, verify planting batch number, record postpartum quality data, input to planting node process file, and construct planting whole-process traceability file.The application is associated with binding by setting agricultural key control point and planting batch number, realizes the automatic acquisition of Epimedium sagittatum planting process data and whole-node traceability.

A method for extracting flavonoids from Epimedium sagittatum is as follows: crushing the Epimedium sagittatum, performing reflux extraction, and recovering under reduced pressure to obtain an extract; suspending the extract in distilled water; and then extracting the extract with petroleum ether, dichloromethane, ethyl acetate, and n-butanol at room temperature to obtain various fractions; separating the dichloromethane fraction, eluting, and combining to obtain fractions Fr.A, Fr.B, Fr.C, Fr.D, Fr.E, Fr.F, Fr.G, and Fr.H; eluting and combining fraction Fr.G to obtain fractions Fr.G1, Fr.G17, and Fr.G17. , Fr.G18; Fraction Fr.G17 was eluted and combined to obtain fractions Fr.G17.1, Fr.G17.15, and Fr.G17.19; Fraction Fr.G17.15 was eluted, recovered under reduced pressure, and combined to obtain fractions Fr.G17.15.1, Fr.G17.15.9, Fr.G17.15.10, and Fr.G17.15.12; Fraction Fr.G17.15.10 was purified by semi-preparative HPLC to obtain compound Epimesatine P; Fraction Fr.G17.15.9 was purified by semi-preparative HPLC, and fractions with different retention times were collected to obtain compounds Epimesatine Q, Epimesatine R, and Epimesatine S, respectively. This method can effectively extract flavonoids from Epimedium sagittatum, can be effectively used to treat breast cancer, and can be used in the preparation of anti-breast cancer drugs, with significant economic and social benefits.

The invention relates to the field of traditional Chinese medicine source extracellular vesicles, and discloses an epimedium source extracellular vesicle as well as a preparation method and application thereof, the plant source of the extracellular vesicle is epimedium sagittatum, and the extracellular vesicle is extracted by an ultrafiltration method. The preparation method comprises the following steps: sample preparation: crushing epimedium and collecting filtrate; sample pretreatment: carrying out gradient centrifugation treatment on the filtrate, and filtering to obtain filtrate; and extracting the extracellular vesicles: enabling the filtrate obtained in the step 2 to sequentially pass through filter membranes with different pore diameters by adopting an ultrafiltration method, and then concentrating by using an ultrafiltration tube to obtain the epimedium extracellular vesicles. In in-vitro tests, the extracellular vesicles prepared by the invention can promote apoptosis of colon cancer cells and inhibit proliferation of colon cancer cells. In a mouse in-vivo experiment, after epimedium extracellular vesicles are treated, the liver and kidney functions are reserved, and the anti-tumor effect does not have systemic toxicity. Research on the action mechanism of the compound shows that the effects of resisting tumor proliferation and delaying the tumor process are achieved through an MEK2-ERK1 / 2 pathway.

The application discloses a SRAP molecular marker primer combination of Epimedium sagittatum 'Qianya No.1' and application thereof, and provides a SRAP molecular marker primer combination sequence for identifying Epimedium sagittatum 'Qianya No.1', wherein the forward primer of Me18-Em10 is 5'-TGAGTCCTTTCCGGTCC-3', and the reverse primer of Me18-Em10 is 5'-GACTGCGTACGAATTCAT-3'. The identified material of the application is not influenced by regional environment, and the target species can be rapidly identified by using one pair of primers.

The invention discloses a method for improving fertilizer conservation and water diversion of epimedium sagittatum cultivation soil. The method comprises the following steps: step 1, deploying a dynamic sunshade and humidity control system; 2, soil improvement; step 3, ridging and ditching; step 4, covering with a mulching film; step 5, transplanting epimedium sagittatum; 6, controlling the sunshade rate; 7, soil humidity control; according to the invention, epimedium sagittatum is planted under the photovoltaic panel, so that solar power generation is utilized, and the cost of specially building a sunshade is saved; the established dynamic sun-shading humidity control system realizes dynamic control of the sun-shading rate by adjusting the angle of a photovoltaic panel, and realizes waterlogging prevention and water saving by collecting rainwater and automatically watering according to the soil humidity; the mulching film is fully covered for water retention and weed suppression, furrows serve as watering channels, water seepage holes of the mulching film are used for water seepage and soil moistening, and the irrigation cost is reduced; epimedium sagittatum herb residues are subjected to high-temperature pyrolysis to prepare biochar, and the biochar is prepared into a soil improvement matrix to improve the soil structure and improve the water drainage, fertilizer retention and moisture retention capacities of soil.

The invention discloses a traditional Chinese medicine composition for treating diabetic erectile dysfunction and application thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition comprises medicine powder and a medicament, the medicine powder comprises the following raw materials: stiff silkworm, centipede and pseudo-ginseng powder; the medicament is prepared from the following raw materials: ginseng, rhodiola rosea, rehmannia, astragalus membranaceus, radix puerariae, caulis spatholobi, loofah sponge, peach kernels, safflower carthamus, angelica sinensis, radix paeoniae alba, pubescent holly roots, morinda officinalis, fructus lycii, herba epimedii and radix bupleuri. The traditional Chinese medicine composition disclosed by the invention has a remarkable treatment effect on diabetes mellitus complicated with erectile dysfunction and diabetic erectile dysfunction, and can be used for obviously improving the erectile function and improving the traditional Chinese medicine syndromes such as low sexual desire, fatigue and fatigue. The traditional Chinese medicine composition has a good curative effect and a certain clinical popularization value in the aspect of treating diabetes mellitus erectile dysfunction.

The present invention relates to a complementary herbal composition for the treatment of oxalate kidney stones, urinary tract infections (UTIs), and sexual dysfunctions. The composition comprises a synergistic blend of herbal ingredients, including Rumex obstusifolius, Lessertia frutescens, Hypoxis hemerocallidea, Gunnera perpensa, Achillea millefolium, Echinacea purpurea, Taraxacum officinale, Epimedium sagittatum, and Rosa canina L., in specific quantities. The formulation is administered in a water base and preserved with Potassium sorbate. PHYTOCASD offers natural, multi-targeted therapeutic benefits, including dissolving oxalate kidney stones, preventing and treating UTIs, and improving sexual function. The invention is intended for use as a complementary medicine, leveraging the diuretic, antimicrobial, anti-inflammatory, and aphrodisiac properties of its ingredients to provide a comprehensive and safe treatment option for individuals suffering from these conditions.

This invention relates to the field of quality information traceability technology, and discloses a method and system for tracing the quality information of Epimedium sagittatum. The method includes acquiring basic data and planting parameters of the current batch of Epimedium sagittatum seedlings; processing the basic data and planting parameters using a hash algorithm to generate a basic query code; using the basic query code as a parent node and combining it with growth data to generate a growth code; generating a raw material code for the current batch by combining it with process parameters, and associating the raw material code with the growth code; generating an outbound code using a timestamp and the raw material code; generating auxiliary material codes for auxiliary materials involved in deep processing, associating the auxiliary material codes with the outbound code, and generating a product code by combining it with production parameters. This invention facilitates rapid identification of the source and responsible link of quality fluctuations, sets incremental collection frequencies and differentiated collection indicators for different stages, improves the sufficiency of quality assessment basis, and reduces the risk of tampering or falsification during the process.

A method for extracting two tocopherol derivatives A and B from Epimedium sagittatum and application thereof can effectively solve the problem of preparing tocopherol derivatives A and B from Epimedium sagittatum and realize the application in preparing anti-breast tumor drugs. The method is as follows: the aboveground part of Epimedium sagittatum is crushed, extracted by refluxing with ethanol, recovered under reduced pressure, extracted, separated by chromatography, eluted by gradient, recovered under reduced pressure, identified by thin layer spotting, combined, purified, collected in the retention time, dried, and obtained. The raw material is abundant, the preparation method is easy to operate, the obtained product is good in quality and good in use effect, can be effectively used for treating breast cancer, realizes the application in preparing anti-breast cancer drugs, develops the medicinal value and commercial value of Epimedium sagittatum, and has remarkable economic and social benefits.

This invention discloses a flavonol glycosyltransferase EsGT_F from *Epimedium sagittatum* and its applications. The EsGT_F gene provided by this invention is 1398 bp in length, encoding 466 amino acids. After cloning the *Epimedium sagittatum* flavonol glycosyltransferase gene EsGT_F, a prokaryotic expression system was constructed. The expressed protein exhibits flavonol glycosyltransferase activity, catalyzing the conversion of cymosin I to icariin. The *Epimedium sagittatum* flavonol glycosyltransferase and its encoding gene provided by this invention offer important gene elements and corresponding technical methods for the microbial cell factory production of the active ingredients of *Epimedium*, and have significant application prospects.

Isopentenyl flavonoid enantiomer compounds are extracted from Epimedium sagittatum and their preparation method and application. The preparation method comprises the following steps: crushing the Epimedium sagittatum to prepare a paste, suspending the paste with water, extracting the paste with petroleum ether, dichloromethane, ethyl acetate and n-butanol respectively to obtain extracts of various parts, performing gradient elution separation by silica gel column chromatography, combining similar parts according to the color development results of thin layer chromatography (TLC) to obtain multiple polar segments; chromatographing the target polar segment and performing gradient elution to obtain multiple components, then combining similar components through thin layer chromatography (TLC) spot plate absorption to obtain multiple sub-components; eluting and purifying the target sub-components, collecting fractions with different retention times, and drying to obtain four compounds respectively. The four compounds have a significant inhibitory effect on the cell viability of human breast cancer MCF-7 cells and have anti-breast cancer activity. The four compounds can be used in the preparation of drugs for treating breast cancer, with huge social and economic benefits.

The invention discloses an epimedium sagittatum cell suspension system as well as a construction method and application thereof, and belongs to the technical field of medicinal plant tissue culture. The construction method of the epimedium sagittatum cell suspension system comprises the following steps: carrying out subculture and suspension culture on epimedium sagittatum calluses to obtain a suspension cell cluster, inoculating the suspension cell cluster into a liquid culture medium, and carrying out shake cultivation to obtain the epimedium sagittatum cell suspension system. On the 6th-8th day of shake cultivation, an elicitor solution is added into the liquid culture medium, and the elicitor comprises jasmonic acid methyl ester, salicylic acid or chitosan. According to the method, the epimedium sagittatum cell suspension system is successfully constructed, the browning problem in the epimedium sagittatum callus culture process is effectively controlled, and a theoretical basis is provided for large-scale culture of epimedium sagittatum so as to solve the current situation problem of short supply of resources at present. Meanwhile, a new direction is provided for improving secondary metabolites of epimedium sagittatum.

The invention discloses an epimedium sagittatum seed embryo after-ripening method. The method comprises the following steps: disinfecting, washing and drying herba epimedii seeds; spraying fluridone onto the river sand, and treating for 24 hours; then putting the river sand into a roller, then putting the epimedium seeds into the roller, and carrying out rolling priming treatment for 24 hours; then gibberellin is used for treatment; the humidity state of the seeds in the roller is observed, and if the seeds are dry, the seeds and the river sand are poured out and treated with gibberellin with the same concentration; the seeds are longitudinally cut at regular intervals, the embryo completion condition is observed until white buds appear, namely endosperm grows out of germs, and embryo after-ripening is completed. According to the method, on the basis of a roller initiation technology, solid media, hormones and medicament treatment are combined, so that epimedium seed embryos are subjected to initiation dynamic treatment continuously in the embryo after-ripening process. According to the method, seed rotting can be overcome, the integrity of cell membranes of the seeds is guaranteed, and the albino seedling phenomenon caused by damage of the cell membranes is avoided. The seed utilization efficiency and the time efficiency are improved, and a foundation is laid for improving the seedling raising efficiency of the herba epimedii.

The utility model belongs to the technical field of field arrangement of epimedium field planting, and discloses a field structure for field planting of epimedium hairy, which comprises a hillside, a plurality of groups of planting grooves are arranged on the surface of the hillside, a drainage groove is arranged at the bottom of each group of planting grooves, convergence grooves are arranged on two sides of the hillside, and the convergence grooves are communicated with the hillside. A water storage tank is formed in the lowest position of the hillside, the gathering groove is communicated with the water storage tank, a plurality of treading blocks are arranged on the surface of the hillside and located between the planting grooves, grooves are formed in the surfaces of the treading blocks, and the grooves are communicated with the water storage tank. The epimedium planting device has the advantages that drainage treatment can be carried out on each group of epimedium planting positions, and water flow is prevented from continuously flowing downwards along the slope, so that the epimedium at the lower part is impacted, plants are destroyed by washing, and the yield of the epimedium is influenced.