84 results about "Icariin" patented technology

The invention discloses an anti-aging herba epimedii extract. The anti-aging herba epimedii extract is prepared by performing microbial fermentation on herba epimedii powder. The invention further discloses a preparation method of the anti-aging herba epimedii extract and application of the anti-aging herba epimedii extract in preparation of anti-aging drugs, food and health care products. According to the invention, through special plant lactobacillus and a fermentation process, efficient conversion of main glycoside components in herba epimedii is realized, the contents of icariin and epimedin C are reduced, the contents of secondary glycoside I and secondary glycoside II are remarkably increased, and new icaritin is generated. The herba epimedii extract disclosed by the invention has the effects of resisting aging and prolonging the service life.

According to the invention, glycosyl transferase UGTBL1 derived from Bacillus licheniformis ZSP01 is taken as a research object, AlphaFold 2 is used for modeling, molecular docking, molecular dynamics simulation and other technologies, amino acid in an active pocket region of glycosyl transferase is analyzed, amino acid in a substrate channel is analyzed by software CAVER3.0, site-directed mutagenesis is carried out, and the glycosyl transferase UGTBL1 is obtained. The regioselectivity and the activity of the mutant for catalytically synthesizing the icariside D2 glycosyltransferase are improved.

This invention proposes an online quality detection method for An Shen Bu Nao Liquid based on multispectral fusion. Addressing the bottlenecks of traditional detection techniques, such as long offline processing time, weak multi-index detection capabilities, and poor dynamic adaptability, this method achieves intelligent quality control throughout the entire production process. The device integrates ultraviolet-visible spectroscopy, near-infrared spectroscopy, and fluorescence spectroscopy modules. It constructs a multi-scale residual convolutional network to extract ultraviolet-visible spectral features, uses a two-way LSTM to analyze the fluorescence excitation-emission matrix, and employs dynamic baseline correction of the ultraviolet spectrum. This improves the detection accuracy of icariin content by 40% compared to traditional HPLC methods, and achieves a heavy metal residue detection limit of 0.05 ppm, providing an innovative solution for the intelligent production of traditional Chinese medicine preparations.

The invention relates to the technical field of traditional Chinese medicine effective component extraction, and provides an efficient extraction and purification process and application of icariin in plateau herba epimedii, the efficient extraction and purification process of icariin in plateau herba epimedii comprises the following steps: S1, epimedium leaves are crushed and sieved to obtain epimedium powder; s2, adding an ethanol solution into the herba epimedii powder, then adding a compound enzyme, performing ultrasonic treatment, inactivating the enzyme, performing suction filtration, and collecting filtrate to obtain an icariin crude extract; s3, diluting the crude icariin extract, adjusting the pH value to 5-6, carrying out chromatographic purification and elution, and collecting the eluate; s4, performing vacuum concentration on the eluent, and performing spray drying to obtain a pure icariin product; the compound enzyme comprises cellulase, pectinase and alpha-mannosidase in a mass ratio of 3: 1: (0.2-1.0). By means of the technical scheme, the problem that in the prior art, the extraction rate of icariin in plateau herba epimedii is low is solved.

The invention discloses a radix astragali, rhizoma polygonati and ligustrum lucidum ait traditional Chinese medicine preparation for treating myelosuppression as well as a preparation method and a detection method thereof. The preparation is prepared from 50-150 parts of radix astragali, 50-150 parts of wine-processed rhizoma polygonati, 25-75 parts of wine-processed ligustrum lucidum ait, 50-150 parts of blanched rhizoma drynariae, 50-150 parts of radix trichosanthis and 25-75 parts of herba epimedii. The optimal extraction process and refining process are screened out based on effective components and pharmacological activity through a large number of tests, and the optimal preparation process of the Shuanghuangliangwhitening oral liquid and other traditional Chinese medicine preparations is obtained through stability investigation and verification. According to the invention, a thin layer chromatography is adopted, the four medicinal materials of astragalus membranaceus, glossy privet fruit, rhizoma drynariae and herba epimedii are respectively subjected to thin layer chromatography research, and methodology research shows that the method is stable and feasible. The invention establishes a content determination method for astragaloside, calycosin-7-glucoside, specnuezhenide and icariin, establishes a quality standard, and has important significance for ensuring the clinical curative effect and safety of astragaloside, calycosin-7-glucoside, specnuezhenide and icariin.

The invention relates to the technical field of medical atomization, and discloses an icariin targeted lung delivery atomization drug delivery device which comprises an atomization main machine and an air guide pipe arranged on the atomization main machine, an atomization cup is arranged at the end, away from the atomization main machine, of the air guide pipe, a mask is fixed to the upper portion of the atomization cup, and the device further comprises an atomization piece and a drug delivery device. The nozzle is fixed at the bottom in the atomizing cup; the screening piece is fixed at the top in the atomizing cup; the seepage piece is fixed in the atomizing cup; an atomizing liquid plate is fixed at the bottom in the atomizing cup, a seepage plate is fixed at the middle position in the atomizing cup, and a permeable plate is fixed at the top in the atomizing cup; the vortex box is fixed below the atomized liquid plate; through collaborative design of airflow driving and the structure, precise screening of target particles of 1-5 microns is achieved, in a screening piece, a coarse screening area of a screening drum forms spiral downward stable vortex airflow in the drum by means of tangent airflow introduced by an air distribution pipe, and liquid beads exceeding 5 microns are thrown to the wall of the drum by means of centrifugal force.

The invention relates to the technical field of pig feeds, and particularly discloses a special compound feed for weaning sows, the feed comprises a functional additive, the functional additive comprises the following raw materials in parts by weight: 1-3 parts of a plant extract compound, 2-5 parts of conjugated linoleic acid, 0.0003-0.0005 part of selenomethionine, 0.15-0.35 part of vitamin E, and 0.15-0.3 part of vitamin C; the plant extract compound is prepared from the following components: astragalus polysaccharide, icariin, epigallocatechin gallate and a carrier, and the mass ratio of the astragalus polysaccharide to the icariin to the epigallocatechin gallate is 1 to (1 to 4) to (0.2 to 0.8). The special compound feed for the weaned sows is used for feeding the weaned sows through a scientific and reasonable formula, can promote follicular development of the sows after weaning, improve the oestrus rate and reduce the proportion of non-oestrus sows, and can solve the problem of slow ovarian function recovery of the sows in summer high-temperature seasons.

The present application relates to a kind of pharmaceutical composition for treating type 2 diabetes and its application.The pharmaceutical composition includes icariin, hydroxymethyl coumarin and cinnamaldehyde, by three-component synergistic effect, simultaneously act on insulin resistance, pancreatic beta cell apoptosis and systemic metabolic inflammation three type 2 diabetes core pathological links, auxiliary reduce serum lipid level, and reduce drug-related liver injury risk, good safety.Animal experiment results show that three-drug combination group can effectively reduce blood sugar (decrease about 50%), significantly improve islet structure atrophy, restore hepatocyte index arrangement, reduce glomerular hypertrophy, significantly reduce serum TG and CHOL level (P<0.05) and inflammatory factor IL-6 and TNF-alpha level (P<0.05), and avoid the hepatotoxicity risk (ALT / AST restores to close to normal level) of hydroxymethyl coumarin.The present application is composed of three kinds of natural monomers with clear structure, and has clear components and controllable quality, which provides a more optimal comprehensive treatment strategy for the treatment of type 2 diabetes and its complications.

The application provides a methyltransferase mutant and application thereof in production of icariin, and belongs to the technical field of bioengineering. The application solves the problem of low icariin yield in the prior art. The methyltransferase mutant is obtained by mutating any one of the 76th position, the 146th position, the 250th position and the 290th position of the amino acid shown in SEQ ID NO. 2. The methyltransferase mutant is mainly used for efficient methyltransferase and application thereof in synthesis of icariin.

The invention provides a daphne giraldii seed composite treatment solution with epimedium polysaccharide as a main effective component and application, and belongs to the technical field of plant seed treatment, the daphne giraldii seed composite treatment solution comprises 100-300 mg / L of gibberellin, 0.1-100 mg / L of epimedium polysaccharide, 15-35 mg / L of 6-benzylamino adenine, 50-150 mg / L of oligosaccharide, 1-30 mg / L of benzothiadiazole, 20-60 mg / L of a bactericide and 0-1 mL / L of a surfactant. According to the application, the daphne giraldii seeds are treated with the daphne giraldii seed composite treating fluid. According to the composite treatment liquid and the treatment method, the germination rate and the germination potential of the seeds can be remarkably improved, mildewing in the stratification period is effectively inhibited, the growth potential of seedlings is enhanced, a standardized and repeatable operation process is provided, and the composite treatment liquid and the treatment method are suitable for large-scale production.

The invention discloses a PH response type modified polydopamine nano preparation loaded with icariin and application of the PH response type modified polydopamine nano preparation in myocardial ischemia resistance, and belongs to the technical field of biological medicine. The nano preparation takes polydopamine (PDA) as a carrier core, the carrier core is modified by methoxy polyethylene glycol (mPEG) and then loaded with icariin (ICA), and the nano preparation is named as mPEG-PDA (at) ICANPs; the preparation process comprises the steps of synthesis of mPEG-OTs, synthesis of mPEG-EDA, synthesis of PDA, preparation of mPEG-PDANPs and preparation of mPEG-PDA (at) ICANPs, under the optimal condition, the particle size of the preparation is 179.6 + / -0.432 nm, PDI is 0.130 + / -0.004, the Zeta potential is 23.4 + / -0.436 mV, the stability is good after the preparation is stored in a dark place at 4 DEG C for 30 days, the ICA drug loading capacity is 14.6 + / -1.08%, the encapsulation efficiency is 64.37 + / -2.31%, and the drug cumulative release rate reaches 73.31 + / -1.64% (pH response drug release) in an environment with the pH value of 6.5 for 72 hours. An in-vitro H9c2 cell OGD model experiment proves that the preparation can remarkably improve the survival rate of ischemic myocardial cells, inhibit apoptosis and reduce LDH release, and the effect of the preparation is superior to that of free ICA. The invention provides an efficient targeting drug delivery system for resisting myocardial ischemia, and has important clinical application value.

The invention discloses a imperatorin and icariin composition and application thereof in preparation of medicines for treating osteoporosis, and belongs to the field of biological medicines. According to the invention, imperatorin and icariin in a mass ratio of 1: (1-5) are prepared into the pharmaceutical composition, and the pharmaceutical composition is applied to treatment of osteoporosis. Cell experiment and in-vivo experiment results show that the pharmaceutical composition provided by the invention has various pharmacological activities of regulating hormone and cell factor levels, resisting osteoclast absorption, promoting osteoblast generation and the like, and can also effectively increase the bone mineralization density of osteoporosis model mice. Based on natural traditional Chinese medicine active ingredients, the medicine which is efficient, low in toxicity and rich in pharmacological activity is provided for treatment of osteoporosis, and the medicine has outstanding clinical application value and wide application prospects.

The invention discloses a preparation for improving male erectile function and sperm quality. The health-care food is characterized by being prepared from the following raw materials in parts by weight: 30-50 parts of herba epimedii extract, 20-40 parts of herba cistanche extract, 15-30 parts of fructus lycii extract, 18-35 parts of semen cuscutae extract, 10-25 parts of herba cynomorii extract, 8-20 parts of maca powder, 6-18 parts of raspberry extract, 0.5-2 parts of lycopene, 1-3 parts of L-carnitine and 0.3-1 part of vitamin B family (vitamin B1, vitamin B2, vitamin B6 and vitamin B12 are mixed according to the mass ratio of 1: 1: 2: 0.5). The herba epimedii extract is selected as a main component, the content of icariin is regulated to be not lower than 60%, the high-content icariin can more effectively promote sex hormone secretion, enhance sexual desire and improve erectile function, the total content of echinacoside and verbascoside in the herba cistanche extract is not lower than 45%, and the herba epimedii extract and the herba cistanche extract have the effect of promoting sex hormone secretion, enhancing sexual desire and improving erectile function. The two components have the effects of tonifying kidney yang and benefiting essence and blood, and can enhance kidney functions.

The invention discloses application of icariin in preparation of drugs for preventing and treating chronic kidney disease mineral substances and bone metabolism disorders. Relates to the technical field of medicine. The invention provides an application of icariin as an active pharmaceutical ingredient in preparation of a CKD-MBD treatment drug. Pharmacological experiments show that icariin can improve renal fibrosis, inhibit hyperparathyroidism, correct calcium and phosphorus metabolism, regulate and control bone metabolism related molecular expression, inhibit transdifferentiation of vascular smooth muscle cells to bone cells, inhibit vascular calcification and correct bone metabolism disorder, so that CKD-MBD is effectively prevented and treated. When icariin is used for preventing or treating CKD-MBD, the icariin is clear in component, exact in curative effect and low in side effect, and has a wide medical application prospect.

The invention relates to application of traditional Chinese medicine herba epimedii, especially an aqueous extract of herba epimedii and epimedin A in prevention and / or improvement of sarcopenia. Compared with a blank control group and a negative control group, the body lengths of the herba epimedii aqueous extract with the concentration of 1 mg / mL, the total flavonoids with the concentration of 0.026 mg / mL, the epimedium A with the concentration of 50 [mu] M, the epimedium B with the concentration of 200 [mu] M, the epimedium C with the concentration of 100 [mu] M, the icariin with the concentration of 200 [mu] M and the icaritin with the concentration of 100 [mu] M are increased by 21.40%, 23.09%, 26.69%, 24.91%, 29.22%, 33.15% and 28.35% respectively. The icariin administration concentration is improved, and the growth promoting effect concentration dependence is obviously enhanced. The body length of the caenorhabditis elegans is increased by 24.19% and even exceeds that of a positive control group. Epimedin A of 50 mu M can effectively increase the body length of caenorhabditis elegans, and other Epimedin A needs higher concentration; 50 [mu] M has the best effect of protecting and relieving the muscular structure. The invention provides a thought for developing medicines for improving dyskinesia and muscle volume reduction, prolonging life, improving muscle fiber atrophy, treating muscle strength decline, tonifying liver and kidney and strengthening muscles and bones, and the medicine is high in safety.

The application discloses a compound nanomicelle co-loading icariin and glycyrrhiza triterpene saponin and a preparation method thereof. The compound nanomicelle comprises icariin, glycyrrhiza triterpene saponin and an amphiphilic block copolymer carrier material, and through a self-assembly process in an aqueous medium, the compound nanomicelle is formed, in which icariin and glycyrrhiza triterpene saponin are simultaneously and stably wrapped by the block copolymer carrier material. The prepared compound nanomicelle has uniform particle size, high encapsulation rate and good stability, can effectively overcome the medicine-making defects of icariin and glycyrrhiza triterpene saponin, and obtain a synergistic pharmacological effect of combination.

The application provides a HPLC-based method for constructing a fingerprint spectrum of Tianyang Qianggu Yin and application thereof, and belongs to the technical field of traditional Chinese medicine detection.The method provided by the application comprises the steps of preparing a test sample solution, preparing a mixed control sample solution, HPLC determination, fingerprint spectrum construction and content determination.The application first establishes a HPLC fingerprint spectrum suitable for water-soluble extracts of Tianyang Qianggu Yin compound, and simultaneously realizes accurate content determination of six components, i.e., neotamanoxin, naringin, alpinumisine A, alpinumisine B, alpinumisine C and icariin.The application has strong specificity, high precision, good repeatability and excellent accuracy.The application provides scientific and controllable technical support for the production quality consistency and efficacy stability of Tianyang Qianggu Yin, and can be used for quality control and batch consistency evaluation of the compound preparation.

The invention belongs to the technical field of pharmaceutical preparations, and relates to an anhydroicaritin nanocrystal oral suspension and a preparation method thereof. The oral suspension contains anhydroicaritin nanocrystals, a suspending aid, a surfactant, a buffer salt system and other pharmaceutically acceptable auxiliary materials. By optimizing the surfactant system and the pH range, the stability of the anhydroicaritin is effectively improved. The problem of long-term stability of a nanocrystalline oral suspension preparation prepared from anhydroicaritin is solved. According to the prescription, the dissolution speed of the medicine is increased, the bioavailability is improved, and the preparation method is easy to operate, stable in process and suitable for industrial production.

The invention relates to application of icaritin in preparation of a medicine for treating bladder cancer. The icaritin shows a good treatment effect in the treatment of bladder cancer regardless of a single drug or a combination of an immunosuppressor and a chemotherapeutic drug.

The invention discloses a formula for tonifying kidney and qi and a prevention and treatment effect of a single medicine of the formula in plateau pulmonary edema. The invention provides application of a kidney-tonifying and qi-benefiting formula or a single medicine or an active ingredient thereof in preparation of a medicine for preventing and / or treating plateau pulmonary edema, the kidney-tonifying and qi-benefiting formula is a traditional Chinese medicinal material composed of herba epimedii, radix astragali and radix rehmanniae recen or an extract of the traditional Chinese medicinal material, the single medicine is herba epimedii, and the active ingredient is icariin. According to the present invention, the plateau pulmonary edema rat model is induced through low pressure and low oxygen, and the prevention and treatment effect of the kidney-tonifying qi-benefiting formula and the single drug or the active component thereof on the plateau pulmonary edema is verified in the animal model, such that the new treatment drug is provided for the clinical treatment of the plateau pulmonary edema, and the limitation of the existing treatment drug is hopefully overcome; good application prospects are realized.

The application relates to a traditional Chinese medicine material component extraction and separation equipment and method based on shengjing capsules, and belongs to the technical field of medicine material component extraction. The equipment comprises an outer cylinder, the top of the outer cylinder is provided with an annular spray pipe with a spray head, and a mixing assembly and a separation assembly are respectively arranged in the outer cylinder and an upper cylinder; the closed feeding and cavity sealing design ensures the stability of the extraction environment and solvent leakage, and provides a prerequisite guarantee for subsequent high-efficiency mass transfer; on the basis, a driving motor synchronously activates a rotating ring, a disturbance plate and a scraper through multi-stage gear transmission; the disturbance plate implements three-dimensional tumbling and shearing on the materials, breaks the medicine material agglomeration, and promotes uniform penetration of the solvent, and the scraper applies periodic extrusion on the bottom layer materials under the damping elastic support, which not only accelerates cell rupture and release of effective components (such as icariin, ginsenosides, polysaccharides of medlar and the like), but also forms an up-down cooperative mechanical field with the disturbance plate in space, so that the initial dissolution rate is obviously improved.

The present application relates to the technical field of traditional Chinese medicine active ingredient extraction, and specifically discloses a method for spatiotemporal separation and selective extraction of active ingredients of Epimedium based on a temperature-controlled phase change barrier. The method uses temperature-controlled phase change microcapsule pre-embedding technology combined with a double-cavity tandem pulse ultrasonic temperature gradient coupled extraction system. First, Epimedium powder is mixed with phase change microcapsules of specific melting point to form a reversible physical release barrier. Then, in the first low-temperature extraction cavity, the microcapsule solid barrier selectively blocks the dissolution of epimedin, while efficiently extracting icariin under the assistance of pulse ultrasonic. The residual target components are locked and degradation is inhibited by rapid cooling of the extraction residue through an inter-stage rapid cooling unit. Finally, in the second high-temperature extraction cavity, the microcapsules melt to release epimedin, and the extraction is completed under the continuous ultrasonic intensification. Through the three-dimensional isolation strategy of "time, space and thermal environment", the extraction rate of icariin can reach more than 90%, and the extraction rate of epimedin can reach more than 85%.

The application discloses a content determination method of Xintong oral liquid, which adopts UPLC method to determine the contents of puerarin, daidzin, 2,3,5,4 ’ tetrahydroxystilbene-2-O-beta-D-glucoside, naringin and icariin in the preparation. The quality detection method is stable and reliable, has strong specificity and good reproducibility, can comprehensively and effectively control the quality of the Xintong oral liquid, is favorable for stabilizing the product quality, ensures the safety and effectiveness of clinical medication, and thus better meets the needs of medical treatment and market.

The invention relates to application of icaritin combined with a pancreatic cancer treatment medicament to preparation of a medicine for treating pancreatic cancer. Compared with a single drug, the drug combination has a better treatment effect.

The invention relates to a preparation method of an icaritin double-cellobiose derivative in the field of medicine and chemical synthesis, which comprises the following steps: icaritin I and bromoacetyl cellobiose II are put into a solvent, and are catalyzed by an inorganic base and a phase transfer catalyst to react to obtain an icaritin double-cellobiose derivative III, and the reaction route is shown in the specification. When the icaritin double-cellobiose derivative III is prepared, icaritin and bromoacetyl cellobiose which are easy to obtain are used as starting raw materials, the price is low, the chemical catalysis process is simple, the reaction condition is mild, the reaction yield is high (36%-76%), and industrial production is easy.

The invention discloses a preparation method of an epimedium sagittatum extract. The preparation method comprises the steps of blanching, back washing, enzyme treatment, ethanol extraction, duplex filtration, column purification and concentration drying. According to the method disclosed by the invention, food-grade sodium carbonate used during blanching is safe and non-toxic, and a waxy coat on the surface of epimedium sagittatum is dissolved by a method of combining blanching and enzyme treatment, so that the problem of blockage of a subsequent duplex filter is solved, and the extraction rate of icariin in the epimedium extract is also improved.

A glycosyl transferase mutant derived from Bacillus licheniformis ZSP01 is used as a research object, AlphaFold 2 is used for constructing a three-dimensional structure of a protein, then an active pocket and a far-end amino acid in the glycosyl transferase are analyzed on the basis of technologies such as a near-attack conformation analysis technology, site-specific mutagenesis and combined mutagenesis are carried out on the glycosyl transferase, and the glycosyl transferase is obtained. The activity of the mutant for catalytically synthesizing the icariside D2 glycosyltransferase is improved.

The invention discloses an icariin enteric-coated sustained-release pellet and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine preparations, and the pellet sequentially comprises a drug-containing pellet core, a sustained-release coating layer and an enteric coating layer from inside to outside. The pellet core contains icariin, microcrystalline cellulose, an adhesive, a disintegrating agent and an antioxidant; the sustained-release coating layer takes ethyl cellulose as a sustained-release material; the enteric coating layer takes acrylic resin and sodium alginate as dual pH response materials, the 2-hour release rate of the pellet in a hydrochloric acid solution with the pH value of 1.0 is less than 10%, the pellet presents near-zero-order release kinetics in a buffer solution with the pH value of 6.8 within 12 hours, the problems that icariin is degraded in gastric acid, the bioavailability is low and the half-life period is short are solved, the icariin can be administrated twice a day, and the bioavailability is high. The traditional Chinese medicine composition is used for treating kidney-yang deficiency type impotence, premature ejaculation and infertility.

The invention discloses a root promoting nutrient solution and a herba epimedii root strengthening and bud increasing seedling raising method, and the method comprises the following steps: performing seed initiation on herba epimedii seeds, performing sand storage and stratification for 60-90 days, and accelerating germination until white buds appear; sowing the white and sprouted seeds on the seedbed, covering the seeds with a substrate, and growing seedlings; when 1-2 true leaves of each seed seedling expand and stem nodes extend to 3-5 cm, terminal buds are completely covered with humic fine soil with the pH being 5.5-6.5, and the soil covering thickness is 1.0-2.0 cm; 2-4 L of a root-promoting nutrient solution is used in each square meter of the seedbed after the seedbed is covered with the soil, and the root-promoting nutrient solution is prepared by adding 150-200 mg of algal polysaccharides, 1.1-1.66 mg of salicylic acid and 800-1200 mg of monopotassium phosphate into each 1 L of water; 30-60 days after the seedbed is covered with soil, when axillary seedlings exist, after more than or equal to 5 secondary roots are formed at the base parts of the axillary seedlings, stem nodes connecting the mother seedlings and the axillary seedlings are transversely cut off in the middle, and the distance between notches and the base parts of new seedlings is 2 + / -0.5 cm; a wound healing nutrient solution is sprayed to notches of the mother seedlings and the new seedlings, and the mother seedlings and the new seedlings are cultured to adult seedlings respectively. The problems that an existing epimedium breeding method is low in breeding coefficient, low in seedling quality and the like are solved.

The invention relates to application of icaritin combined with one or two of a PD-1 antibody and albumin paclitaxel in preparation of a medicine for treating advanced colorectal cancer, particularly colorectal cancer with liver metastasis. The combined medicine can be applied to medicines for relieving advanced colorectal cancer, particularly colorectal cancer with liver metastasis.