The invention relates to a micro-fluidic chip which comprises a chip body and a flow channel structure arranged in the chip body, and a main flow channel of the flow channel structure is sequentiallyprovided with a focusing area, a detection area and a sorting area in the particle flowing direction, wherein the focusing area is used for realizing focusing of particles in the main flow channel ina three-dimensional space, the detection area is used for clamping and detecting target particles, and the sorting area is used for realizing sorting of the target particles and non-target particles.According to the invention, single particles in the continuous flow micro-channel can be fixed at a certain position, time is provided for detection, and the detection of the particles is facilitated;acoustic waves are adopted to push particles in fluid to move, and mechanical force acts on the particles, so that the activity of the particles is not affected; the particles are controlled by adopting sound waves, the sample flux is high, the flow velocity is reduced, and the improvement of the subsequent detection sensitivity is facilitated; the flow channel is simple in structure, the numberof the flow ports is small, and bubble discharging and fluid environment stability maintaining are facilitated.