According to the present invention, based on a liquid droplet
microfluidic chip technology, a
system and a method for low-cost and efficient separation and obtaining of single
cell is designed and constructed. The
system comprises a
microfluidic chip, a liquid droplet
signal detector, a
signal analysis and sorting
control unit, an
electromagnetic valve, a conduct
pipe, and a liquid droplet collection micro-
pipe. The single
cell separation process comprises that: liquid droplets wrapping cells are subjected to online continuous generation and directional flow,
signal acquisition and analysis and sorting control are performed when passing through a
signal acquisition zone, and when the sorting condition (for example: the liquid droplet only warps single
cell) is met, the work of the
electromagnetic valve is controlled to make the force applying state and the flow direction of the liquid droplet be changed so as to separate and collect the single liquid droplet, such that the obtaining of the single cell can be achieved through the de-emulsification, the extraction and other operations. According to the technical method of the present invention, a set of the simple and feasible
system and the simple and feasible method suitable for the liquid droplet sorting,
cell separation and single cell obtaining of different detection methods are provided, and the method has advantages of high efficiency, short time consumption, low cost, no
pollution, wide application range, strong
extensibility and the like compared with the traditional single cell obtaining method.