Micro-fluidic chip used for particle control

Abstract

The invention relates to a micro-fluidic chip which comprises a chip body and a flow channel structure arranged in the chip body, and a main flow channel of the flow channel structure is sequentiallyprovided with a focusing area, a detection area and a sorting area in the particle flowing direction, wherein the focusing area is used for realizing focusing of particles in the main flow channel ina three-dimensional space, the detection area is used for clamping and detecting target particles, and the sorting area is used for realizing sorting of the target particles and non-target particles.According to the invention, single particles in the continuous flow micro-channel can be fixed at a certain position, time is provided for detection, and the detection of the particles is facilitated;acoustic waves are adopted to push particles in fluid to move, and mechanical force acts on the particles, so that the activity of the particles is not affected; the particles are controlled by adopting sound waves, the sample flux is high, the flow velocity is reduced, and the improvement of the subsequent detection sensitivity is facilitated; the flow channel is simple in structure, the numberof the flow ports is small, and bubble discharging and fluid environment stability maintaining are facilitated.

Description

technical field [0001] The invention relates to the field of microfluidic chip, biological particle detection and manipulation technology, and in particular to a microfluidic chip for particle manipulation. Background technique [0002] Microfluidics integrates the basic operation units such as sample preparation, reaction, separation, and detection in the biological, chemical, and medical analysis processes into a micron-scale chip to automatically complete the entire analysis process. Due to its great potential in the fields of biology, chemistry, and medicine, it has developed into a new research field interdisciplinary in biology, chemistry, medicine, fluid, electronics, materials, and machinery. Microfluidic chips are widely used to realize the detection and manipulation of particles. Acoustic tweezers technology based on the principle that particles move to nodes in the standing wave sound field has the function of fixing the position of the particles, so as to facili...

AI Technical Summary

Problems solved by technology

[0003] 1. It is difficult to clamp a single particle in this way. The particles are distributed in the entire flow channel and often gather together under the action of the sound field;

[0004] 2. When the size of the flow channel is large, if the standing wave wavelength is adapted to the size of the flow channel in order to ensure a single node, and the frequency is low and the acoustic radiation force is small, it is difficult to clamp small-sized particles

[0005] 3. When the size of the flow channel is large, in order to ensure the intensity of the acoustic radiation force, it is necessary to have a higher frequency and a smaller sound wave wavelength, so that multiple nodal lines will coexist in the flow channel area, and the particles will flow to different nodal lines, which is difficult To achieve clamping at a certain position

[0007] However, there is no reliable solution in the prior art to meet the needs of particle clamping control

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