10results about How to "Does not affect activity" patented technology

The application discloses an intelligent lifting type culture net cage integrated with environment sensing and adaptive depth adjustment, and belongs to the technical field of marine culture devices. The net cage main body comprises main columns and a net cover. The main columns are provided in plurality. A connecting rod is arranged between the side portions of two adjacent main columns. The periphery of the net cover is supported and tensioned by the main columns to form a closed culture space. A buoyancy adjusting mechanism is connected with the bottom of the main column and is used for adjusting the overall buoyancy of the net cage main body to vertically lift. A mooring positioning mechanism is connected with the buoyancy adjusting mechanism and is used for anchoring the net cage main body in a predetermined sea area and adapting the vertical lifting movement of the net cage main body. An environment sensing module is installed on the net cage main body. An intelligent control module is electrically connected with the environment sensing module and the buoyancy adjusting mechanism. The intelligent control module is internally provided with a decision algorithm, is used for receiving the data collected by the environment sensing module, and outputs a control instruction to the buoyancy adjusting mechanism according to a preset strategy to adaptively adjust the water depth where the net cage main body is located.

The invention relates to a construction method and application of a mouse model with spontaneous gastric precancerous lesions. The construction method comprises the following steps: constructing a Pgc-Cre gene mouse only carrying an inducible Cre gene (tamoxifen can be injected in a rich manner to induce Cre nucleation); the method comprises the following steps: constructing a Rosa26-Zeb2 gene mouse which only carries a Zeb2 gene; the method comprises the following steps: taking Cre and Zeb2 genes as raw materials, mating to obtain a mouse carrying the Cre and Zeb2 genes at the same time, then administering a drug to the mouse, inducing Cre to enter a nucleus and promoting expression of the Zeb2 genes to obtain the mouse model with the spontaneous precancerous lesion of the gastric cancer; the medicine is tamoxifen. The mouse model constructed by the invention is driven by the specific inducible Cre of the stomach main cells, is limited to the stomach main cells, avoids gene disturbance of non-target cells, is simple and convenient to mate, low in cost and short in experimental period, and can be used for screening gastric precancerous lesion drugs.

The invention discloses a hemoglobin-based micro-nano reactor as well as a preparation method and application thereof, belongs to the technical field of biochemistry, and particularly relates to a hemoglobin-based micro-nano reactor which is formed by self-assembly of hemoglobin-polylactic acid conjugates. The hemoglobin-based micro-nano reactor has a cell-like space structure and has multiple functions of a drug delivery system and a micro-nano reactor, hemoglobin biomolecules in the hemoglobin-based micro-reactor are utilized to catalyze atom transfer radical polymerization reaction, and the activity of the hemoglobin-based micro-nano reactor is improved under the condition of not additionally adding a catalyst or gene modification. The micro-nano reactor has the advantages of simplicity and convenience in synthesis, good biocompatibility, various catalytic polymers and the like, and is suitable for the fields of cell engineering, biological material preparation, fluorescence labeling, production of polymers by a fermentation method and the like.

The utility model relates to a mounting device for a vehicle-mounted oxygen generator, which comprises an oxygen generator, an oxygen outlet box, an oxygen supply pipe and a mounting mechanism, a compartment body is placed at the corner of a vehicle trunk, a magic tape piece is positioned on one side of the compartment body, a magic tape hook surface is fixed on one side of the compartment body, and the magic tape hook surface is mounted on the inner wall of the trunk, so that the compartment body is fixed in an auxiliary manner; the supporting plate slides on the two mounting guide rails, the supporting plate moves downwards, 3M glue at the bottom of the supporting plate is pasted in the trunk, the compartment body is fixed to the corner of the trunk, supporting angle seats are arranged at the bent positions of the supporting plate and used for enhancing supporting performance, three oxygen supply pipes are arranged on the surface of the compartment body and communicate with three oxygen outlet boxes correspondingly, and the three oxygen outlet boxes communicate with the supporting angle seats. The oxygen outlet box is installed on the back face of the main driver and the back face of the co-driver through the fixing piece, specifically, the adjusting part is rotated to drive the double-threaded rod to rotate, the two clamping seats are close to each other, grooves in one sides of the clamping seats abut against metal rods at the bottom of a seat headrest, and the oxygen outlet box is fixed.

The application discloses a multifunctional ear moxibustion instrument, which comprises a soft rubber ear fixing body, a heat therapy unit and a music playing module. The soft rubber ear fixing body is made of medical grade silicone or thermoplastic elastomer, and is shaped to be suitable for an auricle, has good flexibility and resilience, can effectively prevent breakage during wearing and taking off, and improves the use safety and product durability. The heat therapy unit is arranged inside or on the surface of the ear fixing body, and is used for warm stimulation of ear acupoints. The music playing module is integrated inside the ear moxibustion instrument or is connected through a wired / wireless mode, and is used for playing audio required by five-element music therapy, so that a patient can receive five-element music therapy while receiving ear moxibustion treatment, and a comprehensive treatment effect of physical and mental treatment is realized. The application has a reasonable structure and convenient operation, is suitable for professional treatment of medical institutions, and is also suitable for daily health care of families, and has a significant market application prospect.

This invention proposes a method for separating biosulfur, comprising: (1) adding an organic flocculant to a mixing tank containing a biosulfurization solution and stirring to obtain a mixed solution; wherein the organic flocculant is selected from at least one of galactosamine, N-acetylglucosamine, and mannosamine; (2) transferring the mixed solution to a settling tank and allowing it to stand to form a supernatant and a bottom precipitate; and (3) separating the supernatant and the bottom precipitate in the settling tank. The method of this invention can achieve efficient separation of biosulfur, with a biosulfur flocculation rate of 95–99 wt%. This invention also provides the application of at least one of galactosamine, N-acetylglucosamine, and mannosamine in the separation of biosulfur.

The application discloses a method for preparing a cell block by using mucin emulsifier and application thereof, and the preparation method comprises the following steps: centrifuging a non-gynecological sample to obtain a first separation liquid, adding the first separation liquid into a formaldehyde-alcohol solution to centrifuge to obtain a second separation liquid, and adding the second separation liquid into mucin emulsifier to centrifuge to obtain a cell block. The preparation method is simple, the manufacturing cost is low, the cell block has high enrichment efficiency, cell loss is small, and the mucin emulsifier does not affect cell activity. The cell block prepared by using the mucin emulsifier has the following advantages: the appearance of the section of the cell block is complete, the section has a regular circular structure, the porosity is moderate, and the thickness is uniform; the application effect of the section in two kinds of staining is good: the hematoxylin-eosin staining has a clear background and clear contrast, cells are uniformly distributed in a circular area, cell morphology presents a stereoscopic effect, and the excellent rate of the stained section is 98.94%; the immunohistochemical staining has a clean background, and structures and cells are specifically dyed, and the excellent rate of the stained section is 99.30%, so that the cell block has a wide application prospect.

The utility model discloses a kind of all-weather electrocardiogram detector of heart failure detection, including detection device, the detection device top end is connected with wearing assembly, the other end of wearing assembly is connected with backplate, the both sides end of backplate width direction is equipped with connecting component, and the other end between detection device of connecting component is detachably connected, adjusting lock catch is further equipped on connecting component.The utility model distributes the weight of entire device on the double shoulder of the person to be detected by setting wearing assembly, and connecting structure is simple, realizes non-inductive wearing, more suitable for long time, all-weather wearing detection, does not affect the normal life and daily exercise of the person to be detected, the spacing after connecting component can be flexibly adjusted detection device and backplate connection, can accurately adjust the degree of fitting of detection device and human body according to the body type state of the person to be detected, so that detection electrode is closely attached to body, improve detection accuracy.

This invention discloses a PTPσ_Ig1-3 fusion protein, its preparation method, and its applications, belonging to the field of genetic engineering. The recombinant fusion protein is obtained by adding an inducible peptide to the N-terminus of the PTPσ_Ig1-3 domain, and sequentially adding a TEV restriction site, GFP fluorescent protein, and a His tag to the C-terminus. The amino acid sequence of the PTPσ_Ig1-3 domain is shown in SEQ ID NO.1, the amino acid sequence of the inducible peptide is shown in SEQ ID NO.2, and the amino acid sequences of the TEV restriction site, GFP fluorescent protein, and His tag are shown in SEQ ID NO.3. Experimental verification showed that this recombinant fusion protein has significant binding activity with glycosaminoglycans such as heparin sodium and chondroitin sulfate, showing promising applications in the study of axonal injury and regeneration mechanisms and in drug delivery therapy.

The invention discloses modified lithium slag for concrete. The content of aluminum sulfate and ferric sulfate in the modified lithium slag is less than 0.1%. According to the modified lithium slag, the content of aluminum sulfate and ferric sulfate in the modified lithium slag is controlled to be smaller than 0.1%, the fluidity of the modified lithium slag is obviously improved, the setting time and activity of the modified lithium slag are not affected, the modified lithium slag can be widely applied to concrete, and the working performance of the concrete is not affected.