1061 results about "Flower pollen" patented technology

The invention discloses a double-dominant SCAR marker for identifying allium fistulosum and allium fistulosum polluted by onion pollen and application of the double-dominant SCAR marker. The specific fragment length of the SCAR marker of an abnormal plant polluted by onion pollen is 1481 bp and 851 bp, the nucleotide sequences of the SCAR marker are shown as SEQ ID NO.1 and SEQ ID NO.2 respectively, the specific fragment length of the SCAR marker of the allium fistulosum is 840 bp, and the nucleotide sequence of the SCAR marker is shown as SEQ ID NO.3; the invention also discloses a specific primer of the SCAR marker. When the marker determined by the method is used for verifying different varieties of green Chinese onions and abnormal plants of the green Chinese onions polluted by the onion pollen, the green Chinese onions and the abnormal plants of the green Chinese onions polluted by the onion pollen can be quickly and stably identified, and compared with a traditional seed purity identification method, the method is not influenced by natural environment and human factors, can be used for batch experiments, improves the identification efficiency, and has a wide application prospect. The invention provides a rapid and feasible method for solving the problem that allium fistulosum varieties are mixed due to the fact that allium fistulosum pollen is polluted by the allium fistulosum pollen cannot be identified in production.

The invention discloses an acer elegans cross breeding method based on flowering phase difference regulation and control, and belongs to the technical field of garden plant breeding. The method comprises the following steps: selecting an acer elegans variety of which the natural early flowering phase difference is greater than or equal to 10 days and the genetic distance is greater than or equal to 0.5 as a cross parent, carrying out low-temperature refrigeration and shading blue light supplementing treatment on a female parent to delay the flowering phase, and carrying out prolonged illumination, red light supplementing and hormone and nutrient solution spraying on a male parent to advance the flowering phase; male parent pollen is collected through a vacuum negative pressure adsorption device and stored in a closed container, a sucrose solution is sprayed to female parent stigmas before pollination, and repeated pollination is conducted through a directional nozzle at 9-11 o'clock every day; carrying out stratification treatment and temperature-difference temperature-control germination on the hybrid seeds, and carrying out light-temperature regulation and control seedling culture after sowing; the molecular marker is used for verifying the hybridization authenticity and target traits, phenotype determination is carried out on three-year-old seedlings to complete breeding, the hybridization obstacle caused by asynchronous flowering phases of the caenorhabditis elegans varieties is solved through the flowering phase regulation and control technology, and the hybridization success rate is increased.

The invention discloses an SCAR marker for identifying onion pollen pollution and application thereof, the specific fragment length of the SCAR marker is 811bp, the nucleotide sequence of the SCAR marker is as shown in SEQ ID NO.1, and the SCAR marker of the onion has no target band; the invention also discloses a specific primer of the SCAR marker. When the marker determined by the method is used for verifying the allium fistulosum of different varieties and the allium fistulosum YZD polluted by the onion pollen, the allium fistulosum and the allium fistulosum YZD polluted by the onion pollen can be rapidly and stably identified, compared with a traditional seed purity identification method, the method is not influenced by natural environment and human factors, batch experiments can be carried out, the identification efficiency is improved, and the method is suitable for large-scale popularization and application. The invention provides a rapid and feasible method for solving the problem that allium fistulosum varieties are mixed due to the fact that allium fistulosum pollen is polluted by onion pollen in production.

The invention provides a humic acid type plant pollen sensitization inhibitor as well as a preparation method and a use method thereof, and the humic acid type plant pollen sensitization inhibitor is prepared from the following raw materials in parts by mass: 1 to 2 parts of polyvinyl alcohol, 0.01 to 0.05 part of guar gum, 2 to 10 parts of acrylic acid, 0.05 to 0.2 part of initiator, 0.5 to 2 parts of potassium humate and 50 to 250 parts of water. The humic acid type plant pollen allergy inhibitor is environment-friendly and low in cost, and has good biocompatibility and allergy inhibition effect.

The invention discloses application of arabinogalactan protein family genes PbrAGP16 in promoting growth of pear pollen tubes, and belongs to the technical field of plant genetic engineering. According to the invention, the gene PbrAGP16 is cloned from pollen of Dangshan pears by using a plant gene cloning technology, and the nucleotide sequence of the gene PbrAGP16 is shown as SEQ ID No.1; the amino acid sequence coded by the gene is SEQ ID No.2 in a sequence table. Researches show that PbrAGP16 can promote growth of pear pollen tubes, the change of PbrAGP16 expression is related to the change of the content of cellulose at the top ends of the pollen tubes, and PbrAGP16 also participates in a regulation mechanism of a self-incompatible reaction. The pollen magnetic transfection technology is adopted to research the function of the gene in the pollen tube, and a wide application prospect is provided for improving the pollination efficiency.

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The invention discloses a preparation method of a composition for dispelling effects of alcohol and protecting liver, and belongs to the technical field of traditional Chinese medicines. The liver protection composition is prepared from the following raw materials: gynostemma pentaphyllum powder, honeysuckle flower powder, hawthorn fruit powder, green tea powder, lotus leaf powder, cassia seed powder and folium sennae powder. The preparation method of the liver protection composition comprises the following steps: enzymolysis, fermentation and freeze-drying. According to the invention, through an extraction method of firstly performing enzymolysis and then performing fermentation, various different types of active effective components are reserved as far as possible. Tests prove that the finally obtained composition has a statistical therapeutic effect on alcoholic liver injury.

The utility model relates to the technical field of plant pollen collection, and discloses a rapid field apocynum venetum pollen collecting device which comprises a pollen scattering device, the pollen scattering device comprises a stamen inlet, a stamen processing pipe is welded to the bottom of the stamen inlet, a pollen scattering and spreading cavity is welded to the bottom of the stamen processing pipe, and the pollen scattering and spreading cavity is communicated with the stamen inlet. Four cavity wall attachment connecting strips are welded to the inner side of the pollen scattering and spreading cavity, a middle rotating shaft is welded to one ends of the four cavity wall attachment connecting strips, a wind driven fan is connected to the inner side of the middle rotating shaft through a rotating shaft, and a pollen scattering brush is welded to the top of the wind driven fan; an impurity filter screen is connected to the middle of the inner side of the pollen scattering and spreading cavity through a bolt, and the problems that according to an existing rapid field apocynum venetum pollen collecting device, pollen falls off through mechanical vibration or airflow blowing, and certain influences are caused to plants in actual use, such as flower bud damage and pollination influence are solved.

The invention belongs to the technical field of biology, and particularly relates to a Yu-grain rpl16 gene and application thereof, in particular to application of the rpl16 gene in cultivation of a rice sterile line. Overexpression of the Yu-grain rpl16 gene in rice can cause rice pollen abortion, so that rice male sterility is realized, the breeding period is shortened, the breeding efficiency is improved, and theoretical guidance is provided for culturing a new male sterility line.

The invention belongs to the technical field of functional food and health food, and particularly relates to a composition with skin whitening and brightening effects and a preparation method thereof. The composition comprises rice peptide, orange osmanthus pollen, white tomato powder, tremella polysaccharide, pomegranate concentrated powder and nicotinamide. Experimental results show that the composition reduces synthesis of melanin by inhibiting the activity of tyrosinase and achieves the effects of whitening and brightening the skin.

This invention describes a new method to generate cereal crop seed. The process involves the delivery of pollen of the male parent at will, as available either in a preserved pollen bank, or using real-time collection from male plants as they become available. Desired pollen is delivered to fertile females during the period when viable pollen from the females and locally proximal unrelated plants is not being released. The delivered male pollen is in such amounts and fortuitously timed that it preferentially pollinates the females. Such fortuitous timing may involve the intentional application of pollen to females a day or two prior to female parent pollen becoming viable, and / or during several periods wherein female parent pollen and / or other proximal plant pollen is not being shed.

The present invention belongs to the technical field of plant pollination devices, and particularly relates to a handheld electric fruit tree pollinator and a pollination method. The handheld electric fruit tree pollinator includes: a depth camera (1), a lateral vibration motor (2), a lateral vibration motor cover (3), a solenoid valve (4), a solenoid valve cover (5), a sealing ring (6), a filter screen (7), a filter screen connecting piece (8), a fluff brush connecting piece (9), a fluff brush (10), a longitudinal vibration motor cover (11), a longitudinal vibration motor (12), a powder chamber cover (13), a powder chamber (14), an elastic rod (15), a screw (16), a multi-joint arm hand rod (17), and an intelligent controller (18); the present invention is convenient to carry and simple to operate; the solenoid valve is closed in the non-working state, reducing pollen waste; based on the pollination principle of insects (bees), the pollen is charged with static electricity through the fluff brush and high-frequency vibration, improving the pollination success rate and reducing the damage to the inflorescence.

The invention discloses a pollen monitoring method based on Fourier-wavelet transform fusion, and belongs to the technical field of environment monitoring, and the method specifically comprises the following steps: S1, carrying out pollen sampling through a sampler, and obtaining a hologram of a pollen sample; s2, performing numerical reconstruction on the obtained hologram, and simulating light propagation by using Fourier transform and a frequency domain transfer function to obtain complex amplitude distribution on a reconstruction plane; s3, performing two-dimensional wavelet decomposition on the reconstructed image, and calculating a wavelet coefficient variance; s4, performing multi-scale depth fusion reconstruction, selecting a wavelet coefficient with the maximum variance by traversing different reconstruction depths, and performing fusion to obtain an optimal reconstruction image; s5, performing post-processing on the reconstructed image; and S6, detecting particles on the processed image, extracting morphological characteristics, and carrying out pollen particle classification, statistics and visualization based on the characteristics. According to the method, the reproduction and identification accuracy of the pollen particles in the lensless digital holographic technology is remarkably improved.

The invention provides an application of an OsINV2 gene in regulation and control of rice cold stress resistance. A nucleotide sequence of the OsINV2 is shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4. By revealing the key regulation and control effect of the vacuole sucrose invertase gene OsINV2 in rice low-temperature stress response, the molecular mechanism that the gene affects the cold resistance of plants by regulating the sugar metabolism balance in vacuoles is illustrated for the first time. The expression of the OsINV2 is regulated in a targeted manner through molecular marker-assisted breeding or a gene editing technology, so that the low-temperature survival rate in the seedling stage and the cold resistance in the booting stage of the rice can be remarkably improved, glumous flower degeneration and pollen abortion caused by low temperature are reduced, and meanwhile, the limitation of the cold resistance of varieties on geographical distribution is broken through; a theoretical support and a technical path are provided for breeding and adaptive popularization of cold-resistant rice varieties.

The invention belongs to the technical field of natural observation, and particularly relates to a pollen propagation monitoring method and device, electronic equipment and a storage medium. When the number of opened monitoring points in a to-be-monitored area is changed, a pollen high-risk sub-area in the to-be-monitored area is evaluated; outputting target monitoring point positions of all pollen sources in each pollen high-risk sub-region; based on any pollen source, collecting a pollen type table of the pollen high-risk sub-region associated with the pollen source; performing positioning early warning on the incidence relation of the pollen sources recorded in the pollen type table, and outputting the early-warned pollen type table; and performing propagation monitoring on pollen in the pollen source, after the propagation monitoring is completed, performing dynamic synchronization on the incidence relation of recording the pollen source in the early-warning pollen type table through the position of the target monitoring point, and outputting the dynamically synchronized pollen type table. According to the invention, the manageability and maintainability of the on-line number change of the monitoring points in the to-be-monitored area can be improved, and the normal pollen risk blocking operation is not influenced.

The invention relates to the technical field of fertilizers, and provides a pollen polysaccharide-based water-soluble fertilizer and a preparation method thereof. The water-soluble fertilizer is prepared from the following components in percentage by mass: 14 to 40 percent of chemical fertilizer, 6 to 10 percent of vegetable protein-based pollen polysaccharide temperature-sensitive hydrogel, 0.1 to 0.5 percent of chlorogenic acid, 1 to 5 percent of humic acid and the balance of water. According to the invention, the pollen polysaccharide is wrapped by the vegetable protein to prepare the temperature-sensitive hydrogel, and the pollen polysaccharide is isolated from inorganic salt in the water-soluble fertilizer; then a stronger hydrogen bond network and electrostatic interaction are formed by carboxyl and multiple hydroxyls in chlorogenic acid molecules, pollen polysaccharide and vegetable protein, so that the stability and the uniform dispersity of a hydrogel system are remarkably improved. The combination of the two improves the flowability of the fertilizer, avoids layering, improves the system consistency during spraying or application, and improves the fertilizer efficiency.

The invention provides a pollen image rapid scanning method under a high-magnification microscope based on retrieval guidance of a low-magnification microscope. The method comprises the following steps: firstly, rapidly scanning a 1000 mm < 2 > region under a whole cover glass through a low-magnification microscope, identifying pollen particles possibly existing in the region, and establishing a prior distribution diagram; and then, in a high-power microscope scanning stage, fine imaging is only carried out on the pollen of the potential target areas, and the areas which are confirmed to be blank are quickly skipped. Compared with a traditional full-area high-power microscope scanning scheme, the intelligent scanning method based on low-power microscope guidance can remarkably reduce invalid imaging time and improve the overall detection efficiency.

According to the method, the pollination problem of an endangered plant, namely the cymbidium serotina, is researched, and in order to improve the pollination rate of the cymbidium serotina and optimally select the pollen collection time, the pollination tool and the pollination time, the invention provides a method for adopting cactus thorns as the pollination tool for the first time, so that the pollination of the cymbidium serotina is facilitated, and the pollination efficiency of the cymbidium serotina is improved. The pollen utilization rate is greatly improved, the breeding number of the precious relict plants is increased, and an efficient pollination method is provided for breeding of the rare and endangered plants.

The invention discloses a method for improving the quality of epimedium seeds and application thereof, and the method comprises the following steps: S1, carrying out fixed-plant and fixed-point observation in a homogeneous garden, recording and observing the flowering habit and synchronism of epimedium delavayi, including the flowering period time and pollination insect species, and observing the flower characteristics; stigma pollination is determined through pollen activity detection, and a basis is provided for subsequent artificial isolated pollination; s2, performing artificial isolation pollination in the flowering phase of the epimedium villosum plants, and using an isolation closing device to prevent cross-pollination. According to the method for improving the quality of the herba epimedii seeds and the application of the method, the flowering rule of the herba epimedii is determined through homogeneous garden transplanting experiments, the pollen viability is dynamically monitored in combination with a TTC staining method, an artificial isolation pollination device and an operation process are designed, and the quality of the herba epimedii seeds is improved. The method has the following technical effects that the seed purity is improved, wherein the seed purity after artificial isolation pollination reaches 90% or above, and the genetic background consistency is remarkably improved.

The invention provides a multi-morphological prior fused pollen particle target detection system and method, and relates to the technical field of pollen detection, and the method comprises the steps: carrying out the feature extraction of a to-be-detected pollen particle image to obtain an initial feature map, and carrying out the convolution of the initial feature map through a pre-constructed morphological convolution kernel library to obtain a morphological response feature map; performing adaptive weighted feature fusion based on the morphological response feature map to obtain a morphological confidence map, and processing fusion features obtained based on the morphological confidence map and global context features extracted from the initial feature map to obtain a spatial attention map; and carrying out feature re-calibration on the initial feature map by using the space attention map to obtain an enhanced feature map, and then carrying out target detection on the enhanced feature map to obtain pollen particle information output. The method has the advantages that the problems that automatic pollen detection background interference is large and forms are difficult to distinguish are effectively solved, and high-time-efficiency pollen concentration monitoring is supported; and the defects that the traditional manual microscopic examination process is complicated, long in period and depends on professionals are overcome.

The invention discloses a rice pollen collecting device and method, and belongs to the technical field of hybrid rice seed production. The pollen suction device comprises a pollen suction nozzle, a suction gun, a pollen accumulation kettle and a pollen separation assembly. The powder suction nozzle can be replaced and is suitable for different wind speed environments; the suction gun is used for providing suction negative pressure; the pollen accumulation kettle is detachably connected with the input end of the suction gun, a pollen suction nozzle communicated with the outside is arranged on the pollen accumulation kettle, and a one-way collection unit and a refrigeration unit which are used for blocking pollen output are arranged in the pollen accumulation kettle; the pollen separation assembly comprises a pollen separation frame body arranged in the pollen accumulation kettle and a vibration unit, the exterior of the pollen separation frame body is movably connected with the inner wall of the pollen accumulation kettle, and a plurality of electrostatic pipelines are formed on the pollen separation frame body; the vibration unit is arranged on the pollen separation frame body. High-speed pollen can be intercepted in advance, and it is avoided that the pollen blocks the filtering device. The pollen can be quickly collected on the premise of keeping the pollen viability, the collection efficiency is remarkably improved, and meanwhile the device has the advantages of being safe, portable and the like.

The invention provides an intelligent plant growth state evaluation and analysis system and method, and belongs to the technical field of state evaluation, and the system comprises a position determination module which is used for obtaining the vegetation information and geographic position of a target plant; the state determination module is used for acquiring a climate information data set during the planting period of the target plant, and determining first growth state data of the target plant according to the vegetation information and the climate information data set; the image acquisition module is used for acquiring a growth image of the target plant for image analysis to obtain second growth state data of the target plant; the state evaluation module is used for evaluating the growth state of the target plant according to the first growth state data and the second growth state data, and the release amount determination module is used for determining the pollen release amount of the target plant according to the growth state evaluation result of the target plant and the vegetation information of the target plant. By combining the vegetation information and the geographic position, the accuracy of growth state evaluation can be effectively improved.

The invention discloses a lettuce LsNP1 gene and application of the lettuce LsNP1 gene in preparation of male sterile plants. Specifically, the invention discloses a protein LsNP1 with an amino acid sequence of SEQ ID NO: 1 and application of a coding gene of the protein LsNP1 in forming a male sterility phenotype of lettuce. Experiments show that the number of pollen grains of the mutant obtained after the LsNP1 gene is knocked out is obviously reduced, the pollen activity is obviously reduced, the pollen grains are abnormal in shape, and anther cannot normally scatter and inseminate during flowering, so that lettuce forms a male sterility phenotype. The male sterile lettuce is successfully created, valuable gene resources are provided for germplasm resource innovation and genetic improvement of the lettuce, a new field is developed for application of the LsNP1 gene, and a new material is provided for application of a lettuce male sterile line. By utilizing the gene and the method, the lettuce male sterile line germplasm can be rapidly created, the cultivation process of a new lettuce variety is accelerated, and the gene and the method have a wide application prospect in the field of agricultural production.

The invention relates to the technical field of allergy detection, in particular to an inhalation allergen specific IgE antibody detection kit and application thereof. The kit is a colloidal gold detection kit. The detection objects are pollen, dust mites and animal scurf; the kit comprises a colloidal gold labeled mouse anti-human IgE antibody arranged on a test strip combination pad; the pollen antigens (Art v1, Amb a1 and Bet v2), the dust mite antigens (Der f1 and Der p1) and the animal scurf antigen (Can f1) are arranged on a nitrocellulose membrane of the test strip. The kit provided by the invention can be used for rapidly identifying the allergen of the inhalation allergy symptom and classifying the severity of the allergy symptom, and the identification result has relatively high accuracy.

Tree pollen allergy is seasonal allergy caused by various tree pollen spread in air. The allergy affects a substantial proportion of populations, is particularly significant in European and North America areas, and is characterized by symptoms such as sneezing, nasal obstruction, rhinorrhea, eye itching and lacrimation, and throat irritation. The symptoms typically occur in spring, at which birch, alder, hazel and oak release pollen into the air. Tree pollen allergy is a type 1 hypersensitivity reaction mediated by an immunoglobulin E (IgE) antibody. The present invention relates to antibodies and antigen binding fragments thereof that bind to one or more of tree pollen allergens Bet v 1, Aln g 1, Cor a 1 and Quue a 1, resulting in inhibition of IgE-mediated anaphylaxis.

The present invention discloses a primary biomass fiber-pollen blended bioplastic and its preparation method and application, relating to the field of bioplastic technology. The preparation method of the bioplastic is as follows: taking natural fiber, crushing it, adding water and stirring to form a fiber suspension; taking natural pollen to clean and remove impurities and lipids on the surface, using alkali solution for stirring treatment, washing the mixed system to neutrality and filtering to remove excess water to obtain pollen microgel; mixing the fiber suspension and pollen microgel evenly to obtain a mixed slurry; applying the mixed slurry on a mold, drying and peeling it off to obtain primary biomass fiber-pollen blended bioplastic. The primary biomass fiber-pollen blended bioplastic provided by the present invention has high mechanical strength, can be easily water-shaped and water-spliced, and has good recyclability and biodegradability; its preparation method utilizes a large amount of waste or low-value-added biomass resources, has a simple process, low production cost, and less environmental pollution.

The invention discloses application of a brassica juncea gene BjuABCG42-A03 in regulating and controlling pollen germination and seed setting amount of brassica juncea, and relates to the technical field of plant genetic engineering, the amino acid sequence of the protein BjuABCG42-A03 is as shown in SEQ ID No.1, and meanwhile, the invention provides a method for cultivating a transgenic plant capable of improving the pollen germination rate or seed setting amount. The method comprises the following steps: constructing a recombinant plant expression vector containing a gene for coding protein BjuABCG42-A03; the constructed recombinant plant expression vector is transformed into agrobacterium and then transformed into a plant or a plant cell, and the gene of the coded protein BjuABCG42-A03 is over-expressed in the plant, so that the pollen germination rate or the seed setting amount of the plant is increased. Based on gene information of arabidopsis ABCG42 (At4g15233), a BjuABCG42-A03 gene is homologous cloned in a mustard genome, in-vivo and in-vitro germination experiments show that the BjuABCG42-A03 gene affects the elongation length and seed bearing quantity of a pollen tube, and a research direction is provided for solving the problem of low pollen fertility or low seed setting quantity of mustard subsequently.

A pollen collection device for collecting pollen from crop plants grown in rows includes a housing assembly configured to be mounted on a base for being transported through a row of crop plants. The housing assembly receives at least a portion of the plants as the housing assembly is transported through the row of crop plants. An agitation assembly is attached to the housing assembly for agitating the plants as the housing assembly is transported through the row of crop plants to displace pollen from the plants. A pollen collection assembly is attached to the housing assembly for collecting the pollen displaced from the plants as the housing assembly is transported through the row of crop plants.

The present invention relates to the field of allergens and more specifically to a novel isolated allergen from the species Cupressaceae shown to be a primary sensitizer for severe peach allergy mediated by the protein Pru p7. The allergen is homologous with Pru P7 in peach, a protein belonging to the Gibberelin Regulating Proteins (GRPs). The novel allergen also 5 shares high sequence homology among other species from the Cupressaceae family and there is therefore also provided additional novel allergens in two other species of the Cupressaceae family. The novel allergens find use in the in vitro diagnosis of, treatment and / or prevention of Type 1 Cupressaceace pollen allergy and Cupressaceace pollen-associated food allergies.

The utility model provides novel tweezers for artificial emasculation and pollination of tomatoes. The novel tweezers comprise a left tweezers arm and a right tweezers arm which is symmetrical to the left tweezers arm, wherein one end of the left tweezers arm and one end of the right tweezers arm are gradually close to each other to form a connecting end, the other ends of the left tweezers arm and the right tweezers arm are gradually away from each other to form a clamping end, and an arc-shaped tweezers body part is connected between the clamping end and the connecting end; a connecting spring is arranged between the left tweezers arm and the right tweezers arm, and a pollen storage structure is arranged at the clamping end. Through the arrangement of the pollen storage structure, male parent pollen can be conveniently collected, more pollen can be contained, a plurality of stigmas can be pollinated at the same time through the pollen amount, the pollination efficiency is improved, the left tweezers arm and the right tweezers arm are elastically opened and closed through the arrangement of the connecting spring, and the pollination efficiency is improved. During use, stamens of female parents can be quickly taken out by utilizing the elasticity of the connecting spring, so that the castration efficiency is improved.