1643 results about "Flower pollen" patented technology

A method for deleting a region of DNA in a plant. In some embodiments, the method comprises transforming a plant with a nucleic acid molecule, wherein the nucleic acid molecule encodes one or more zinc finger nuclease(s) (ZFNs) operably linked to one or more tissue-specific promoter(s), e.g., a pollen-specific promoter. Methods include excising native genes in a plant. Accordingly, in some embodiments, ZFNs are engineered that recognize sequences that flank native plant genes. In further embodiments, ZFNs are expressed under the control of developmental stage-specific promoters, such that, for example, nucleic acid sequences are specifically excised in plants during relatively late stages of development. Nucleic acid molecules useful for carrying out disclosed methods and plants produced by the methods are included.

The invention discloses a preparation method of porous spherical biological carbon and an application. The method comprises the following steps: firstly, carbonizing pollen by hydrothermal reaction; secondly, burning the carbonized pollen under inert atmosphere protection; and finally carrying out washing, suction filtration and drying on the burnt product to obtain the porous spherical biological carbon. According to the method, the technology is simple and easy to control; carbonization and activation of the pollen can be well achieved under the premise of keeping the original structure; and spherical biological carbon with different pore structures can be obtained by controlling the reaction temperature. Furthermore, the biological carbon prepared by the method can be applied to a lithium/sodium-ion battery and a super capacitor, can be used as an adsorbent, a template agent and a carbon substrate in a composite material, and especially demonstrates excellent electrochemical properties when applied to the lithium/sodium-ion battery.

The invention discloses pollen suspension suitable for the liquid pollination of fruit trees and a preparation method thereof, belonging to the technical field of pomiculture. The required pollen suspension is obtained by two steps, namely sieving pollen suspended components and sieving components keeping the viability of pollen in liquid. The invention can well suspend and evenly distribute the pollen to be well suspended, and the pollen can keep higher viability in a liquid solution; the invention can be widely applied to the liquid pollination of fruit trees and can realize the manual liquid pollination operation with large area, thereby overcoming the difficulty of sterilitas of the self flowers or low fruition rate of the self flowers of tree species in production; and the invention enhances the pollination quality, promotes fructification, saves labour and enhances the efficiency.

The invention discloses a method for creating rice engineering maintainer lines preventive against gene flow. The method includes the steps of 1, site-specifically inserting an exogenous reporter gene and a pollen lethal gene into a flanking sequence of a fertility control gene of a wild rice chromosome so as to obtain a transgenic line containing the exogenous reporter gene and the pollen lethal gene, wherein the exogenous reporter gene, the pollen lethal gene and the fertility control gene are able to closely link and inherit; 2, hybridizing wild rice and homozygous recessive rice common genic male sterile lines to obtain F1-generation plants, hybridizing the F1-generation plants as male parents to the transgenic line to obtain a generation F2, screening the generation F2 to obtain hybrid lines containing the exogenous reporter gene, namely rice engineering maintainer lines required, wherein the homozygous recessive rice common genic male sterile lines are mutant lines with the sterility control gene. The invention further discloses application of the rice engineering maintainer lines preventive against gene flow in rice breeding.

The invention provides a preparation method and product of instant particles of bee pollen active ingredient and products thereof. The wall of bee pollen is broken using a composite enzymolysis wall-breaking method and the wall-broken bee pollen and the common excipients are processed into the instant particles. The bee pollen is subjected to enzymolysis by the plant composite hydrolase, it is a newest technological process, with marked features such as simpleness, mild condition, easy operation, short time-consumption of reaction process, high wall breaking rate, compared with the other technological process. After the bee pollen is subjected to enzymolysis, the content of the main active ingredient is markedly increased and the sensitizer is subjected to enzymolysis, therefore the nutritive value of the product is greatly increased and the food safety of the bee pollen product is increased and the product quality is increased.

The invention provides meat rabbit feed comprising raw materials in part by weight as follows: 20-30 parts of corn, 8-10 parts of soy beans, 8-10 parts of sesame cake, 8-10 parts of rape seed cake, 10-12 parts of brewer's grains, 15-20 parts of wheat bran, 15-20 parts of soybean straw powder, 8-12 parts of common vetch bran, 4-6 parts of alfalfa meal, 10-12 parts of unite bran, 4-6 parts of edible fungi bran, 4-6 parts of cassia twig powder, 4-6 parts of waste sugar residue, 1-3 parts of polygonum powder, 4-6 parts of pear flower, 4-6 parts of jasmine flower, 4-6 parts of moringa oleifera leaf powder, 4-6 parts of dry aloe powder, 0.01-0.03 part of dry cactus powder, 0.01-0.03 part of dry spirulina powder, 1-3 parts of ginkgo biloba powder, 4-8 parts of honey, 0.5-0.8 part of stone powder, 0.5-1.0 part of egg-shell powder, 0.2-0.5 part of montmorillonite powder, 0.4-1.0 part of common salt, 0.05-0.1 part of blackberry flower powder, 0.02-0.04 part of rosemary pollen, 0.01-0.03 part of medicated leaven powder, 0.01-0.03 part of bupleurum, 0.01-0.03 part of common fennel powder, 0.1-0.3 part of dandelion powder, 1-3 parts of pomegranate rind powder, 1.5-2.5 parts of composite vitamin premixe, 0.001-0.002 part of decarboxylase powder and 0.001-0.002 part of robenidine. Fed by the feed provided by the invention, meat rabbits quickly grow, have low morbidity and are good in meat quality.

The invention relates to a preparation method, seed propageation method and application of seeds of a rice male sterile line. The preparation method comprises the following steps: mutating an OsMS4 gene sequence in the rice seeds by using routine methods, and acquiring a rice male sterile line with a mutation in homozygous recessive OsMS4 gene by traditional genetic hybridization screening. The OsMS4 sterile line can recover pollen fertility under certain seed propageation conditions, and has the capacity of propagating the seeds containing the mutation in the homozygous OsMS4 gene. The seeds containing the mutation in the homozygous OsMS4 gene can realize seed propageation. According to the invention, by controlling the rice cultivation time to regulate the reproductive growth of rice, the fertility and sterility of the rice male sterile line, that is, the OsMS4 plant can be controlled, one line with dual purposes can be realized, and the production process of hybrid rice seeds is simplified.

The invention discloses a seedless grosvenor momordica with high mogroside content, high whole fruit utilization rate and good taste, and a cultivating method for the seedless grosvenor momordica. The steps of the method are as follows: 1) cultivating and planting the diploid female and male seedlings of the grosvenor momordica; 2) when a male plant emerges a flower bud, inducing the formation of diploid big pollen; 3) picking the male flower that is induced to bloom and screen a diploid big pollen therein; 4) hybridizing the haploid oocyte of the diploid female plant after treating the diploid big pollen in the pollen germination solution, acquire the hybrid seeds after the female plant fruits; 5) propagating the hybrid seed into whole plant and then screening a triploid plant with the Chromosome number inspection; 6) planting the triploid plant and the diploid male plant, and carrying out artificial pollination to the male triploid plant with the male diploid plant when the plant is blooming, thus acquiring the seedless grosvenor momordica when the female plant fruits.

The invention provides a method for effectively reducing environmental risk caused by pollen propagation of transgene plant. In the method, two tightly linked gene expression cassettes of 1) a pollen lethal gene ZM-AA1 driven by a pollen growth anaphase singular promoter PG47 and 2) a fluorescence screening mark gene FP driven by a callus / seed coat singular promoter END 2 are transferred to a Zhonghua 11 wild type material. During selfing fructification of a single plant carrying a single copy transgene, a pollen grain without carrying the transgene can be fertilized with a female gamete normally, but a pollen grain carrying the transgene is abortive in pollen growth anaphase and can not be fertilized with a female gamete, so as to reduce risk of transgene drift in the environment.

The invention discloses a double-glue pollen food with functions of enriching the blood, beautifying the face and nourishing the skin and a preparation method thereof, belonging to the field of functional foods. The double-glue pollen food uses donkey-hide glue, fish skin glue and pine pollen or bee pollen as main raw materials which are matched with honey, oligosaccharides (bifidus factors), ferrous gluconate nutrition enhancers and auxiliary materials to prepare granular preparations or tablets by a scientific formula and fine processing. The donkey-hide glue, the fish skin glue, the pollenand other plentiful nutrient components are organically combined with iron, the bifidus factors and nutrition enhancing factors, thereby fully exerting the nutrient and health care functions of the donkey-hide glue, the fish skin glue and the pollen. The product has sweet taste and can be carried conveniently. The invention solves the problem of complicated eating of the donkey-hide glue, the fish skin glue and the pollen for a long time. The double-glue pollen food can achieve the effects of enriching the blood, beautifying the face and nourishing the skin after being eaten for a long time.

Provided is a method for producing a rice F1 seed, including crossing a rice male sterile line of Koshihikari containing one or more genes selected from the group consisting of the sd1 gene, the Gn1 gene and the hd1 gene derived from Oryza sativa L. cultivar Habataki, or a rice male sterile line exhibiting semi-waxiness as a seed parent, with a rice fertility restorer line as a pollen parent, and collecting the first filial generation seed (F1 seed) from the post-crossing seed parent; and a rice male sterile line containing one or more genes selected from the group consisting of the sd1 gene, the Gn1 gene and the hd1 gene derived from Oryza sativa L. cultivar Habataki.

The invention relates to a preparation method for a carbon-film-coated zinc oxide hollow sphere. Pollen is taken as a template for preparing a ZnO hollow sphere, and sericin is used as a carbon source for performing carbon film coating; the condition does not exist before the application; the preparation method comprises the steps of fully mixing zinc salt with an ethanol solution of pollen, and adding alkali liquid to a water bath in a dropwise manner; performing aging, centrifuging and separating to obtain ZnO precipitate with a pollen template; performing high temperature sintering on dried white powder to obtain the ZnO hollow sphere; immersing the ZnO hollow sphere powder into a sericin solution, and performing centrifuging, separating and drying; and performing vacuum sintering on the dried powder to obtain the carbon-film-coated ZnO hollow sphere taking the sericin as the carbon source. The preparation method is low in cost, simple in process, low-carbon and environment-friendly; and the obtained carbon-film-coated ZnO hollow sphere can be used as a lithium ion battery negative electrode material, a photocatalytic material and the like.

The invention relates to genetic engineering and molecular biology, and particularly discloses a plant anther specific promoter PCHF15 and application thereof. The invention further provides an expression vector with the plant anther specific promoter PCHF15, a genetic expression box and a carrier with the genetic expression box. The invention also provides a primer pair for amplifying the plant anther specific promoter PCHF15. The plant anther specific promoter PCHF15 is an endogenous gene of rice, is beneficial to genetic engineering of the rice, and drives exogenous genes to accurately express specificity in pollen, so that a novel method for driving specificity expression of the exogenous genes in the pollen is provided.

Compositions are provided that include date palm pollen or an extract thereof. The compositions may be formulated for topical use in methods of treatment or prophylaxis of various skin conditions or methods to counteract the effects of skin aging.

The invention provides traded rabbit feed which comprises raw materials in part by weight as follows: 10-15 parts of barley, 10-15 parts of corn, 8-10 parts of sunflower seed cake, 8-10 parts of rape seed cake, 10-12 parts of brewer's grains, 15-20 parts of wheat bran, 15-20 parts of peanut vine powder, 8-12 parts of cereal germ root, 4-6 parts of lucerne powder, 12-15 parts of unite bran, 4-6 parts of edible fungi bran, 4-6 parts of orange meal, 4-6 parts of waste sugar residue, 1-3 parts of herba schizonepetae powder, 4-6 parts of pear flower, 4-6 parts of jasmine flower, 4-6 parts of moringa oleifera leaf powder, 4-6 parts of brassica juncea, 0.01-0.03 part of dry cactus powder, 0.01-0.03 part of dry spirulina powder, 1-3 parts of garlic leaf powder, 4-8 parts of honey, 0.5-0.8 part of stone power, 0.5-1.0 part of egg-shell powder, 0.2-0.5 part of lobster shell powder, 0.4-1.0 part of common salt, 0.05-0.1 part of blackberry flower powder, 0.02-0.04 part of lanceolata flower powder, 0.01-0.03 part of medicated leaven, 0.01-0.03 part of radix bupleuri powder, 0.01-0.03 part of common fennel powder, 0.1-0.3 part of rhizoma polystichi powder, 1-3 parts of pomegranate rind powder, 1.5-2.5 parts of composite vitamin premixes, 0.001-0.002 part of decarboxylase powder, and 0.001-0.002 part of chloroquanidi hydrochloride. Fed by the feed provided by the invention, traded rabbits have strong immunity and can grow much and good fur, and in addition, the breeding cost of the traded rabbits is greatly lowered.

Plant powder, plant bag, and dried plant are created with a wide range of various ingredients. Plant powder, plant bag, and dried plant are created comprising at least one plant ingredient, with at least one flavoring. At least one plant ingredient having nutrients including carbohydrates, vitamins, minerals, and antioxidants. At least one plant ingredient is in form selected from group consisting of plant powder, plant bag, and dried plant. At least one plant ingredient is selected from group consisting of herb, tea, root, flower, flower bud, pollen, stem, stem shoot, leaf, bulb, cereal grain, nut, seed, bean, fruit, fruit skin, vegetable, and vegetable skin. At least one plant ingredient can further comprising addition of at least one nutritional ingredient, and at least one food additive. At least one flavoring is selected from group consisting of bland, sweet, sour, bitter, salty, spicy, and pungent.

The invention discloses a seed production method for a hybrid pepper variety. The seed production method comprises the following steps of: manually removing an anther of a pepper female parent, collecting pollen of a peeper male parent, and carrying out artificial supplementary pollination on the pepper female parent, wherein the produced F1 generation seed is a hybrid seed; the step of manually removing the anther of the pepper female parent is carried out through adopting a manual rotation method; the manual rotation method comprises the following steps: a joint position between a pedicel and a receptacle is pinched by a left hand; a position, where is close to the receptacle, of a corolla is gently pinched by a thumb and a forefinger of a right hand; and the thumb and the forefinger slowly twist, so that the corolla and the anther are removed, and a style and an ovary are exposed. A female parent emasculation method (manual rotation method) disclosed by the invention has the characteristics of saving labor and force, capably reducing production cost, realizing thorough emasculation, and capably ensuring emasculation quality; and a stamp-pad ink marking method is adopted by the invention, and the stamp-pad ink cost is low, so that the production cost can be reduced, as well as labor and force are saved. The conventional marking method is an iron ring marking method; the ironring marking method is realized through bonding an iron ring on a pollinated stalk, so that the operation cost is high; and the iron ring has a certain weight, so that flowers are easy to fall off, therefore the yield is low.

The invention relates to a toxin-expelling weight-reducing flower cake and a preparation method thereof. The flower cake comprises the following raw materials by weight: 15%-50% of pollen, 20%-70% of glutinous rice flour or flour, 1%-2% of bifidus factors, 10%-20% of oil, 2%-10% of water, 2%-5% of other auxiliary materials, 0.1%-0.5% of vanillin, and 0.05%-0.2% of aspartame. The toxin-expelling weight-reducing flower cake is obtained by mixing and braising the above raw materials. The toxin-expelling weight-reducing flower cake obtained by the method in the invention has flower fragrance and agreeable sweet taste, has bowel-relaxing and toxin-expelling efficacy, can reach the effect of maintaining beauty and keeping young after long-term eating, and is convenient for both eating and carrying. Toxin-expelling weight-reducing flower cake series products can be manufactured according to different taste and functions of flowers.

A Chinese medicine for treating prostatic tumor is prepared from rapes pollen through extracting in organic solven and purifying by silica gel column.

The invention discloses honey pollen paste which comprises the following components in parts by mass: 5-10 parts of lycium barbarum, 2-5 parts of Chinese angelica, 5-10 parts of longan aril, 47-57 parts of honey, 25-35 parts of bee pollen, 1 part of propolis, 10-15 parts of royal jelly and 0.02-0.04 part of organic selenium. The honey pollen paste disclosed by the invention consists of four components, namely the lycium barbarum, the Chinese angelica, the longan aril and the organic selenium, as well as the honey, the propolis, the royal jelly and the bee pollen, the components are reasonably matched together and supplement with one another, and the problem of single nutrition ingredients of the conventional pollen paste is solved. The pollen paste is reasonable in formula and simple in process, the prepared honey pollen paste is comprehensive in nutrition ingredients, and the bee pollen has a remarkable health-care effect and well exerts the nutrition and health-care effects.

The invention discloses an artificial pollination breeding method of a pinus massoniana hybrid. The artificial pollination breeding method comprises the following steps of 1, selection of masson pine as a female parent, 2, selection of pinus elliottii, pinus elliottii-pinus caribaea hybrid, pinus caribaea and pine moth-resistant pinus massoniana as male parents, 3, collection of male parent pollen, 4, treatment on the male parent pollen, 5, bagging and artificial pollination by a home-made pollination tool, and 6, cultivation. Through the artificial pollination breeding method, seeds of the pinus massoniana hybrid having the excellent and stable properties of straight trunk, fast early-stage growth rate, high rosin yield and pest and disease resistance are obtained. The artificial pollination breeding method solves the problems of open pollination in a pinus massoniana seed garden and improves crossbreeding efficiency. The artificial pollination breeding method is a complete artificial pollination breeding method, has simple processes, is practical, has a low cost, low requirements on operators, and small influences by a natural environmental factor such as weather, can effectively improve cross pollination efficiency in a pinus massoniana seed garden, can improve accuracy and reliability of cross pollination, can accelerate seed selection of a novel forestall germplasm resource variety, and has good economic, social and ecological benefits.

The present invention discloses a feed used for breeding of honeybees. The feed comprises the following components in percentages by weight: 20-24% of dry rape flower pollens, 18-22% of sucrose, 16-20% of milk powder, 12-18% of defatted soybean powder, 10-14% of distilled water, 5-8% of honey, 4-6% of calcium carbonate, 3-5% of magnesium sulfate, 3-5% of edible salt and 2-4% of compound vitamins.Starting from needed nutrients and appetite of the honeybees, the feed used for the breeding of the honeybees relatively well provides required nutrients and taste of the honeybees, enables the honeybees to grow rapidly, speeds up a honey production of the honeybees, reduces breeding costs of the honeybees, and improves breeding economic benefits of the honeybees. Besides, the produced honey is rich in nutrients, high in nutritional value and improved in quality.

The invention provides a producing technique of natural pollen fermenting products. The producing technique of the natural pollen fermenting products utilizes an advanced aerodynamical smashing technique and biological fermenting apparatus and technique. Natural pollen is raw materials. The natural pollen can be smashed and extine of the natural pollen can be broken by utilizing aerodynamical force and pollen protein can be degraded into amino acid and polypeptide by adding probiotic fermentation microorganism. A natural pollen fermenting product can be obtained, wherein the natural pollen fermenting product has rich nutrition and massive probiotic materials. The natural pollen fermenting products finally can be processed into products after drying and smashing. The producing technique of the natural pollen fermenting products can overcome various defects of the natural pollen. The obtained product is fine and smooth in taste and moderate in sour and sweet without bitter and stink. The natural pollen fermenting products can reduce stomach discomfort rate of human body caused by eating the pollen and reducing blood fat effect of the natural pollen fermenting products is better than the natural pollen obviously.

The present invention relates to in planta transformation methods for plants or plant materials with a genetic construct, wherein at least one meristematic cell of a meristematic tissue of an immature inflorescence able to differentiate into a gamete of a pollen or of an ovule is exposed and then transformed, wherein the transformation can be performed to yield a stable integration or to yield a transient introduction of a genetic construct of interest. Further provided are methods for manufacturing a transgenic plant, or methods for manufacturing a genetically manipulated plant based on the in planta transformation methods according to the present invention. In addition, there is provided a plant or a progeny thereof manufactured according to the transformation and/or manufacturing methods according to the present invention.