758 results about "Flower pollen" patented technology

The invention discloses a double-dominant SCAR marker for identifying allium fistulosum and allium fistulosum polluted by onion pollen and application of the double-dominant SCAR marker. The specific fragment length of the SCAR marker of an abnormal plant polluted by onion pollen is 1481 bp and 851 bp, the nucleotide sequences of the SCAR marker are shown as SEQ ID NO.1 and SEQ ID NO.2 respectively, the specific fragment length of the SCAR marker of the allium fistulosum is 840 bp, and the nucleotide sequence of the SCAR marker is shown as SEQ ID NO.3; the invention also discloses a specific primer of the SCAR marker. When the marker determined by the method is used for verifying different varieties of green Chinese onions and abnormal plants of the green Chinese onions polluted by the onion pollen, the green Chinese onions and the abnormal plants of the green Chinese onions polluted by the onion pollen can be quickly and stably identified, and compared with a traditional seed purity identification method, the method is not influenced by natural environment and human factors, can be used for batch experiments, improves the identification efficiency, and has a wide application prospect. The invention provides a rapid and feasible method for solving the problem that allium fistulosum varieties are mixed due to the fact that allium fistulosum pollen is polluted by the allium fistulosum pollen cannot be identified in production.

The invention provides a humic acid type plant pollen sensitization inhibitor as well as a preparation method and a use method thereof, and the humic acid type plant pollen sensitization inhibitor is prepared from the following raw materials in parts by mass: 1 to 2 parts of polyvinyl alcohol, 0.01 to 0.05 part of guar gum, 2 to 10 parts of acrylic acid, 0.05 to 0.2 part of initiator, 0.5 to 2 parts of potassium humate and 50 to 250 parts of water. The humic acid type plant pollen allergy inhibitor is environment-friendly and low in cost, and has good biocompatibility and allergy inhibition effect.

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

This invention describes a new method to generate cereal crop seed. The process involves the delivery of pollen of the male parent at will, as available either in a preserved pollen bank, or using real-time collection from male plants as they become available. Desired pollen is delivered to fertile females during the period when viable pollen from the females and locally proximal unrelated plants is not being released. The delivered male pollen is in such amounts and fortuitously timed that it preferentially pollinates the females. Such fortuitous timing may involve the intentional application of pollen to females a day or two prior to female parent pollen becoming viable, and / or during several periods wherein female parent pollen and / or other proximal plant pollen is not being shed.

The invention discloses a pollen monitoring method based on Fourier-wavelet transform fusion, and belongs to the technical field of environment monitoring, and the method specifically comprises the following steps: S1, carrying out pollen sampling through a sampler, and obtaining a hologram of a pollen sample; s2, performing numerical reconstruction on the obtained hologram, and simulating light propagation by using Fourier transform and a frequency domain transfer function to obtain complex amplitude distribution on a reconstruction plane; s3, performing two-dimensional wavelet decomposition on the reconstructed image, and calculating a wavelet coefficient variance; s4, performing multi-scale depth fusion reconstruction, selecting a wavelet coefficient with the maximum variance by traversing different reconstruction depths, and performing fusion to obtain an optimal reconstruction image; s5, performing post-processing on the reconstructed image; and S6, detecting particles on the processed image, extracting morphological characteristics, and carrying out pollen particle classification, statistics and visualization based on the characteristics. According to the method, the reproduction and identification accuracy of the pollen particles in the lensless digital holographic technology is remarkably improved.

The invention provides an application of an OsINV2 gene in regulation and control of rice cold stress resistance. A nucleotide sequence of the OsINV2 is shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4. By revealing the key regulation and control effect of the vacuole sucrose invertase gene OsINV2 in rice low-temperature stress response, the molecular mechanism that the gene affects the cold resistance of plants by regulating the sugar metabolism balance in vacuoles is illustrated for the first time. The expression of the OsINV2 is regulated in a targeted manner through molecular marker-assisted breeding or a gene editing technology, so that the low-temperature survival rate in the seedling stage and the cold resistance in the booting stage of the rice can be remarkably improved, glumous flower degeneration and pollen abortion caused by low temperature are reduced, and meanwhile, the limitation of the cold resistance of varieties on geographical distribution is broken through; a theoretical support and a technical path are provided for breeding and adaptive popularization of cold-resistant rice varieties.

The invention provides a pollen image rapid scanning method under a high-magnification microscope based on retrieval guidance of a low-magnification microscope. The method comprises the following steps: firstly, rapidly scanning a 1000 mm < 2 > region under a whole cover glass through a low-magnification microscope, identifying pollen particles possibly existing in the region, and establishing a prior distribution diagram; and then, in a high-power microscope scanning stage, fine imaging is only carried out on the pollen of the potential target areas, and the areas which are confirmed to be blank are quickly skipped. Compared with a traditional full-area high-power microscope scanning scheme, the intelligent scanning method based on low-power microscope guidance can remarkably reduce invalid imaging time and improve the overall detection efficiency.

The invention provides a multi-morphological prior fused pollen particle target detection system and method, and relates to the technical field of pollen detection, and the method comprises the steps: carrying out the feature extraction of a to-be-detected pollen particle image to obtain an initial feature map, and carrying out the convolution of the initial feature map through a pre-constructed morphological convolution kernel library to obtain a morphological response feature map; performing adaptive weighted feature fusion based on the morphological response feature map to obtain a morphological confidence map, and processing fusion features obtained based on the morphological confidence map and global context features extracted from the initial feature map to obtain a spatial attention map; and carrying out feature re-calibration on the initial feature map by using the space attention map to obtain an enhanced feature map, and then carrying out target detection on the enhanced feature map to obtain pollen particle information output. The method has the advantages that the problems that automatic pollen detection background interference is large and forms are difficult to distinguish are effectively solved, and high-time-efficiency pollen concentration monitoring is supported; and the defects that the traditional manual microscopic examination process is complicated, long in period and depends on professionals are overcome.

The invention relates to the technical field of allergy detection, in particular to an inhalation allergen specific IgE antibody detection kit and application thereof. The kit is a colloidal gold detection kit. The detection objects are pollen, dust mites and animal scurf; the kit comprises a colloidal gold labeled mouse anti-human IgE antibody arranged on a test strip combination pad; the pollen antigens (Art v1, Amb a1 and Bet v2), the dust mite antigens (Der f1 and Der p1) and the animal scurf antigen (Can f1) are arranged on a nitrocellulose membrane of the test strip. The kit provided by the invention can be used for rapidly identifying the allergen of the inhalation allergy symptom and classifying the severity of the allergy symptom, and the identification result has relatively high accuracy.

Tree pollen allergy is seasonal allergy caused by various tree pollen spread in air. The allergy affects a substantial proportion of populations, is particularly significant in European and North America areas, and is characterized by symptoms such as sneezing, nasal obstruction, rhinorrhea, eye itching and lacrimation, and throat irritation. The symptoms typically occur in spring, at which birch, alder, hazel and oak release pollen into the air. Tree pollen allergy is a type 1 hypersensitivity reaction mediated by an immunoglobulin E (IgE) antibody. The present invention relates to antibodies and antigen binding fragments thereof that bind to one or more of tree pollen allergens Bet v 1, Aln g 1, Cor a 1 and Quue a 1, resulting in inhibition of IgE-mediated anaphylaxis.

The invention discloses application of a brassica juncea gene BjuABCG42-A03 in regulating and controlling pollen germination and seed setting amount of brassica juncea, and relates to the technical field of plant genetic engineering, the amino acid sequence of the protein BjuABCG42-A03 is as shown in SEQ ID No.1, and meanwhile, the invention provides a method for cultivating a transgenic plant capable of improving the pollen germination rate or seed setting amount. The method comprises the following steps: constructing a recombinant plant expression vector containing a gene for coding protein BjuABCG42-A03; the constructed recombinant plant expression vector is transformed into agrobacterium and then transformed into a plant or a plant cell, and the gene of the coded protein BjuABCG42-A03 is over-expressed in the plant, so that the pollen germination rate or the seed setting amount of the plant is increased. Based on gene information of arabidopsis ABCG42 (At4g15233), a BjuABCG42-A03 gene is homologous cloned in a mustard genome, in-vivo and in-vitro germination experiments show that the BjuABCG42-A03 gene affects the elongation length and seed bearing quantity of a pollen tube, and a research direction is provided for solving the problem of low pollen fertility or low seed setting quantity of mustard subsequently.

The present invention relates to the field of allergens and more specifically to a novel isolated allergen from the species Cupressaceae shown to be a primary sensitizer for severe peach allergy mediated by the protein Pru p7. The allergen is homologous with Pru P7 in peach, a protein belonging to the Gibberelin Regulating Proteins (GRPs). The novel allergen also 5 shares high sequence homology among other species from the Cupressaceae family and there is therefore also provided additional novel allergens in two other species of the Cupressaceae family. The novel allergens find use in the in vitro diagnosis of, treatment and / or prevention of Type 1 Cupressaceace pollen allergy and Cupressaceace pollen-associated food allergies.

The present application relates to the technical field of genetic engineering, and particularly relates to application of overexpression of BnA05CARK3 gene in regulation of fertility of Brassica napus. The present application successfully constructs a recombinant vector for overexpression of BnA05CARK3 gene by using the cloned BnA05CARK3 gene, and makes the BnA05CARK3 gene in Brassica napus to be genetically transformed and overexpressed by means of agrobacterium infection, and then promotes the development process of Brassica napus under drought stress environment, so that the pollen vitality and the thousand-grain weight of Brassica napus can be improved, and the quality and yield can reach the normal development degree of wild type Brassica napus.

The present invention aims to treat hay fever by providing water from which pollen (an allergen) has been removed using the pollen treatment facility C of the present invention. [Solution] In a water treatment system consisting of a water tower B, a water treatment plant D, a sand filtration area E, and a water distribution reservoir F, a pollen treatment facility is provided to remove pollen from the river water 1 supplied to the water treatment plant D.

The utility model discloses a kind of pollen collecting devices, belong to pollen collection field, including collecting component and extension component, collecting component includes air suction barrel, the side fixed connection of air suction barrel has installation cylinder, air suction fan is installed in the inside of installation cylinder, air suction barrel is fixedly connected with mounting head at the opposite side of installation cylinder, the end of mounting head is detachably installed with collecting pipe, the side surface of collecting pipe is equipped with push brush, the lower end of air suction barrel is installed with collecting hopper, filter screen is installed in the inside of collecting hopper, the collecting component and extension component of being set by the utility model not only can be convenient to the collection and filtering operation of pollen, and can avoid causing damage to pistil, simultaneously can be convenient to the discharging of pollen, and then can enhance the practicality of collecting device, simultaneously, through the above structure cooperation, it is also convenient to the pollen collection operation of different height pistil, and then can improve the use convenience of collecting device.

The invention relates to the technical field of agricultural biology, in particular to a related lncRNAPB.235.1 for regulating pollen development and application of the related lncRNAPB.235.1. According to the invention, a wheat photo-thermo-sensitive male sterile line BS366 is used as an experimental material, the related lncRNAPB.235.1 for regulating anther development is obtained and introduced into arabidopsis thaliana, and the result shows that anther development is abnormal. The long-chain non-coding RNA for regulating anther development has very important theoretical and practical significance for improving plant varieties and shortening the crop harvesting period.

The utility model relates to the technical field of artificial pollination, in particular to an auxiliary tool for artificial pollination, which comprises an end cover for containing collected pollen; the conical soft baffle is made of a soft material, a small port of the conical soft baffle is bonded with a front side port of the end cover, and meanwhile, a large port of the conical soft baffle faces forwards; the opening and closing framework is installed on the peripheral side of the outer wall of the end cover, the conical soft block is installed on the opening and closing framework, the conical soft block is fully opened to form a large space for corn tassels to easily enter, then the corn tassels can be wrapped by closing a large end opening of the conical soft block, and at the moment, when the tassels are shaken, pollen can be fully received.

The application discloses a kind of blending fertilizer raw material potash fertilizer and its preparation method, including the following weight parts of raw materials: 980 parts of potassium sulfate powder, 3 parts of zinc sulfate monohydrate, 10 parts of magnesium sulfate heptahydrate, 2 parts of sodium octaborate tetrahydrate, 1 part of DA-7, 4 parts of alginic polysaccharide.Seven water magnesium sulfate replenishes magnesium element, promotes the synthesis of carbohydrates, fats and proteins, is essential to maintain the green and healthy growth of crop leaves, and is conducive to improving the overall growth vigor and yield of crops.Four water sodium octaborate provides boron element, can promote pollen germination and pollen tube elongation, improve the flowering rate of crops, reduce the phenomenon of flower and fruit drop, DA-7 can promote plant cell division and elongation, improve the photosynthetic efficiency of plants, enhance the stress resistance and nutrient absorption capacity of plants, make crops grow more robustly, and improve fertilizer utilization.

The present application relates to the technical field of plant genetic breeding, and particularly relates to a Brassica campestris fatty acyl reductase BcFAR2 gene, an expression protein thereof and application, a base sequence of the Brassica campestris fatty acyl reductase BcFAR2 gene is shown as SEQ ID NO. 1, and an amino acid sequence of the expression protein is shown as SEQ ID NO. 2, the Brassica campestris fatty acyl reductase BcFAR2 gene is cloned, and BcFAR2 gene overexpression Brassica campestris sterile plants can restore pollen activity; gene knockout Brassica campestris fertile plants can significantly reduce pollen activity; in Arabidopsis thaliana, overexpression of the gene can significantly improve salt tolerance of the transgenic plants; and in Brassica campestris, overexpression of the gene can significantly enhance salt tolerance.

A device for distributing pollen attachable to a drone, characterized in that it comprises: - a hopper (1) that stores a quantity of pollen, equipped with an agitator that ensures the homogeneity of the pollen, - at least one outlet orifice (8), located at the bottom of the hopper (1), - a metering lever (2) located below the outlet orifice (8), equipped with a displacement mechanism that regulates the degree of opening of the outlet orifice (8) and therefore the quantity of pollen that comes out through it, - a Venturi tube (3) provided with a central narrowing corresponding to the outlet orifice (8), - a blower (5) connected to one end of the Venturi tube (3), equipped with a motor, which provides pressure along the Venturi tube (3) to facilitate the exit of the pollen, - at least one adjustable nozzle (4) connected to the other end of the Venturi tube (3), which allows the dispersion of the pollen, - a supporting structure (7) linked to the hopper (1) which is equipped with a plurality of couplings (6) intended to be coupled to a drone, - a control unit connected to the displacement mechanism of the metering lever (2) that regulates the pollen output and connected to the blower motor (5), - a power supply or a connecting element intended to connect the device to a power supply of the drone.

The invention belongs to the technical field of hybrid rice breeding, and discloses a method for cultivating a long-grain late-japonica black-fragrant-glutinous rice three-line sterile line, which comprises the following steps: S1, selecting a short-round-grain early-maturing japonica rice local germplasm with black brown rice seed coat and fragrance, and hybridizing with short-round-grain late-japonica glutinous rice to obtain a hybrid; s2, the hybrid seeds are subjected to multiple cross with long-grain fragrant high-yield high-quality tasty late japonica rice, long-grain black fragrant glutinous rice excellent strains are selected from multiple cross advanced generations to enter a strain yield identification test, the excellent strains are selected as recurrent parents to be subjected to test cross with a long-grain late japonica sterile line, and a sterile test cross group is obtained; s3, sterile plants similar to the recurrent parents are selected from the sterile test cross population and subjected to continuous backcross transformation with the recurrent parents, the long-grain late-japonica black-fragrant-glutinous rice three-line sterile line which is regular and consistent in population, thorough and stable in pollen abortion and good in flowering habit is obtained, and the corresponding recurrent parents are maintainer lines; the problems that the yield of black rice is low and the cooking taste quality is poor are solved.

System for recording data on allergic reactions of a vehicle occupant, comprising a recording and / or storage device (5, 30) by means of which pollen in the area of ​​the vehicle (1) can be recorded and / or stored, wherein the recording and / or storage device (5, 30) has an electrically controllable pollen inlet (34) with which the pollen recording can be controlled.

The utility model relates to collecting device technical field, and disclose a pollen collecting device, including the box, the inside middle place rotation of box is installed with the turntable, the turntable is provided with the uniformly distributed sector groove, the inside of sector groove all places has the collecting box, the outside of turntable is fixed and is connected with the external gear in the near upper side department, the inside bottom of box is rotatory and is installed with the transmission shaft in the near right side middle department, the outside of transmission shaft is fixed and is connected with the worm wheel in the near lower side department, the inside of box is rotatory and is installed with the worm in the front side department of transmission shaft, the meshing between worm and worm wheel, the outside of transmission shaft is fixed and is connected with the main gear in the near upper side department. The device can be according to actual demand flexible rotation turntable, will be accurately placed in the corresponding collecting box below the external thread pipe, realizes the classification collection of different kinds of pollen, is helpful to subsequent targeted research and use to pollen, reduces the cumbersome process of classification arrangement.

The invention relates to the technical field of forest cultivation, and discloses a carya illinoensis fruit forest variety configuration method which comprises the following steps: configuring a main cultivation variety and a pollination variety in a fruit forest; preparing a pollination synergistic composition; the composition is sprayed at the initial stage of stigma exposure of female flowers of a main cultivated variety. The composition comprises a phenolic acid grafted carboxymethyl chitosan derivative, low methoxyl pectin, a calcium ion cross-linking agent, sugar alcohol chelated boron and spermidine. According to the method, an interfacial film with antioxidant activity is formed on the surface of the stigma by using the grafting modified matrix, so that the stigma browning is effectively inhibited, and the pollination period is prolonged; by constructing a calcium cross-linked low-methoxyl pectin interpenetrating network, an interfacial film is endowed with an intelligent degradation characteristic of enzyme-sensitive response to pollen, and protection at ordinary times and immediate dissolution after touching flowers are realized. According to the method, pollen is captured through static electricity, nutritional factors are released in a targeted mode, the pollination and fertilization efficiency is remarkably improved, the sterility risk in the flowering phase is relieved, and the yield and quality of carya illinoensis are improved.

The utility model relates to the technical field of fructus amomi pollination, and discloses a fructus amomi supplementary pollination device which comprises a base, an outer sleeve is fixedly connected to the left side of the top end of the base, an air pump is fixedly connected to the right side of the top end of the base, a storage box is arranged at the top of the base, and an adjusting assembly for powerful supporting is arranged above the outer sleeve. The adjusting assembly comprises an outer connecting plate, and an inner adjusting rod is vertically arranged at the top of the outer sleeve in a penetrating mode. According to the auxiliary pollination device for fructus amomi, by arranging an inner adjusting rod, an outer sleeve, an external connecting plate, a supporting plate, a placement frame, a sliding block and an auxiliary groove, the flexibility of a pollen suction head is improved, the structural adaptability is enhanced, the position and the opening state of a fructus amomi flower can be accurately recognized, and the pollination effect is improved. The problems that an existing auxiliary pollination device for fructus amomi is low in pollen collection efficiency, poor in pollen activity keeping, low in pollination precision, low in automation degree, high in cost and the like are solved.

The invention discloses a method for carrying out nine-fold breeding by utilizing female gametes, and relates to the technical field of biological breeding. Comprising the following steps: step 1, pollinating hexaploid persimmon pollen to a'rattan royal house 'containing 2n eggs; 2, collecting young fruits which are born for 65-70 days after pollination, stripping seeds, putting the seeds into a sterilized triangular flask, treating the seeds for 30 seconds in an ultra-clean workbench by using 70% ethanol, pouring out the seeds, adding a 1% NaClO solution, soaking the seeds for 10 minutes, and shaking the seeds once every 3-4 minutes. According to the method for carrying out nine-fold breeding by utilizing female gametes, in persimmon breeding, compared with a 2n pollen path, the 2n egg path is higher in feasibility, 2n eggs and complete male germplasm with good pollen traits are hybridized, pollination populations are expanded, and polyploidy seedless materials can be obtained.

The invention belongs to the technical field of plant germplasm resource preservation, and provides a special protective agent for pollen integrated treatment of Chinese honey locust hybrid flowers, a kit and an integrated treatment method thereof. The protective agent is prepared from 8%-12% w / v of trehalose, 0.05%-0.08% v / v of Triton X-100 and a phosphate buffer solution with the pH value of 7.0. The integrated treatment method comprises the following steps: performing stamen morphology primary screening and separation on the Chinese honey locust flowers at low temperature; grinding in an ice bath by using a protective agent, filtering, centrifuging and enriching fertile pollen; quickly evaluating the activity of the split-flow part suspension by using an MTT method; carrying out trehalose-tert-butyl alcohol gradient dehydration and low-temperature vacuum drying on the residual part to obtain dried pollen, and storing for a long time; and treating with rehydration liquid before use. The extraction damage is reduced to the maximum extent; tert-butyl alcohol and trehalose synergistically dehydrate and dry, so that the pollen safely enters a vitrification state, and long-term stable preservation is realized; the repeatability is high, standardized operation is achieved, and a reliable tool is provided for gleditsia sinensis germplasm resource preservation and breeding.

1. The name of the design product: inflator powder sprayer. 2. The use of the design product: for spraying pollen for artificial pollination and for spraying termites and insecticide powder for pest control. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.