1334 results about "Flower pollen" patented technology

The invention discloses a double-dominant SCAR marker for identifying allium fistulosum and allium fistulosum polluted by onion pollen and application of the double-dominant SCAR marker. The specific fragment length of the SCAR marker of an abnormal plant polluted by onion pollen is 1481 bp and 851 bp, the nucleotide sequences of the SCAR marker are shown as SEQ ID NO.1 and SEQ ID NO.2 respectively, the specific fragment length of the SCAR marker of the allium fistulosum is 840 bp, and the nucleotide sequence of the SCAR marker is shown as SEQ ID NO.3; the invention also discloses a specific primer of the SCAR marker. When the marker determined by the method is used for verifying different varieties of green Chinese onions and abnormal plants of the green Chinese onions polluted by the onion pollen, the green Chinese onions and the abnormal plants of the green Chinese onions polluted by the onion pollen can be quickly and stably identified, and compared with a traditional seed purity identification method, the method is not influenced by natural environment and human factors, can be used for batch experiments, improves the identification efficiency, and has a wide application prospect. The invention provides a rapid and feasible method for solving the problem that allium fistulosum varieties are mixed due to the fact that allium fistulosum pollen is polluted by the allium fistulosum pollen cannot be identified in production.

The invention discloses an acer elegans cross breeding method based on flowering phase difference regulation and control, and belongs to the technical field of garden plant breeding. The method comprises the following steps: selecting an acer elegans variety of which the natural early flowering phase difference is greater than or equal to 10 days and the genetic distance is greater than or equal to 0.5 as a cross parent, carrying out low-temperature refrigeration and shading blue light supplementing treatment on a female parent to delay the flowering phase, and carrying out prolonged illumination, red light supplementing and hormone and nutrient solution spraying on a male parent to advance the flowering phase; male parent pollen is collected through a vacuum negative pressure adsorption device and stored in a closed container, a sucrose solution is sprayed to female parent stigmas before pollination, and repeated pollination is conducted through a directional nozzle at 9-11 o'clock every day; carrying out stratification treatment and temperature-difference temperature-control germination on the hybrid seeds, and carrying out light-temperature regulation and control seedling culture after sowing; the molecular marker is used for verifying the hybridization authenticity and target traits, phenotype determination is carried out on three-year-old seedlings to complete breeding, the hybridization obstacle caused by asynchronous flowering phases of the caenorhabditis elegans varieties is solved through the flowering phase regulation and control technology, and the hybridization success rate is increased.

Disclosed are methods of preserving pollen such that the pollen has improved viability as compared to pollen that is left in ambient conditions. A method of the present invention includes separating dead pollen contents from live pollen grains. In some embodiments, pollen grains are stored with a substance that prevents dead pollen contents from interacting with live pollen grains. The substance may be a solid, liquid, gas, or combination thereof. In some embodiments, the substance may at least partially regulate pollen moisture content, such as maintaining pollen moisture content at 15-60%.

The invention discloses an SCAR marker for identifying onion pollen pollution and application thereof, the specific fragment length of the SCAR marker is 811bp, the nucleotide sequence of the SCAR marker is as shown in SEQ ID NO.1, and the SCAR marker of the onion has no target band; the invention also discloses a specific primer of the SCAR marker. When the marker determined by the method is used for verifying the allium fistulosum of different varieties and the allium fistulosum YZD polluted by the onion pollen, the allium fistulosum and the allium fistulosum YZD polluted by the onion pollen can be rapidly and stably identified, compared with a traditional seed purity identification method, the method is not influenced by natural environment and human factors, batch experiments can be carried out, the identification efficiency is improved, and the method is suitable for large-scale popularization and application. The invention provides a rapid and feasible method for solving the problem that allium fistulosum varieties are mixed due to the fact that allium fistulosum pollen is polluted by onion pollen in production.

The invention provides a humic acid type plant pollen sensitization inhibitor as well as a preparation method and a use method thereof, and the humic acid type plant pollen sensitization inhibitor is prepared from the following raw materials in parts by mass: 1 to 2 parts of polyvinyl alcohol, 0.01 to 0.05 part of guar gum, 2 to 10 parts of acrylic acid, 0.05 to 0.2 part of initiator, 0.5 to 2 parts of potassium humate and 50 to 250 parts of water. The humic acid type plant pollen allergy inhibitor is environment-friendly and low in cost, and has good biocompatibility and allergy inhibition effect.

The invention relates to a system for constructing an insert element comprising the following components: - one or more cutting elements designed to cut at least four frame elements from them and to use the at least four frame elements (2, 3) to replicate the shape of the rectangular door or rectangular window and to adapt the insert element to the dimensions of the door or window in this way; four corner elements, wherein the frame elements can be connected to the corner elements to form a rectangular frame; - at least one protective tarpaulin, the protective tarpaulin is designed to span the area limited by the frame; - at least one fastening strap with at least one adhesive element, wherein the protective cover can be attached to the frame using at least one fastening strap in such a way that the fastening strap wraps around the frame in such a way that it forms two tabs projecting from the frame which fix the protective cover with the adhesive element. The insert element can be an insect screen, pollen screen or sun protection element.

Disclosed herein are methods for preparing sporopollenin exine capsules (SECs) and methods for preparing composite materials that comprise SECs that utilize ionic liquid compositions. The composite materials typically include structural polymers and the SECs, and the SECs optionally may encapsulate useful materials, such as flame retardant materials, phase change materials, and therapeutic materials, such as probiotics and prebiotics. The composite materials may be prepared from ionic liquid compositions comprising the structural polymers and the SECs which optionally may encapsulate the useful materials, where the ionic liquid is removed from the ionic liquid compositions to obtain the composite materials comprising the SECs.

The invention discloses application of arabinogalactan protein family genes PbrAGP16 in promoting growth of pear pollen tubes, and belongs to the technical field of plant genetic engineering. According to the invention, the gene PbrAGP16 is cloned from pollen of Dangshan pears by using a plant gene cloning technology, and the nucleotide sequence of the gene PbrAGP16 is shown as SEQ ID No.1; the amino acid sequence coded by the gene is SEQ ID No.2 in a sequence table. Researches show that PbrAGP16 can promote growth of pear pollen tubes, the change of PbrAGP16 expression is related to the change of the content of cellulose at the top ends of the pollen tubes, and PbrAGP16 also participates in a regulation mechanism of a self-incompatible reaction. The pollen magnetic transfection technology is adopted to research the function of the gene in the pollen tube, and a wide application prospect is provided for improving the pollination efficiency.

The invention relates to the technical field of pollen polysaccharides, and discloses a preparation method and application of rape pollen polysaccharides, and the preparation method comprises the following steps: treating degreased rape pollen with a mixed enzyme solution of cellulase and pectinase, and centrifuging to obtain a polysaccharide-containing supernatant; mixing the supernate with absolute ethyl alcohol, centrifuging, and dissolving the precipitate with distilled water to obtain a polysaccharide solution; the polysaccharide solution is sulfonated and modified by a chlorosulfonic acid and pyridine mixed solution, and then carboxymethylated with a monochloroacetic acid and NaOH mixed solution to obtain a modified polysaccharide solution. And finally, dialyzing to remove impurities, and freeze-drying to obtain the rape pollen polysaccharide. Through the extraction and modification technology, the application range of polysaccharide products in agriculture is expanded, crop growth can be more effectively promoted, the yield and quality are improved, and meanwhile negative influences on the environment are reduced.

The invention provides a pollen monitoring method and device based on a convolutional neural network and the convolutional neural network, and relates to the technical field of pollen monitoring, and the specific implementation mode comprises the steps: collecting an original pollen image, and inputting the original pollen image into the convolutional neural network; and processing the original pollen image data through the convolutional neural network to obtain a feature image, and outputting a pollen type identification and classification result based on the feature image. According to the method, the low-order visual features of the pollen are extracted through the convolutional neural network, so that the network can capture key morphological features more effectively; through multistage convolution operation, deeper features can be extracted from a pollen image layer by layer, global morphological information can be gradually abstracted from local geometric features, pollen types with slight differences can be distinguished through a gradually refined feature extraction process, multiple pollen types can be accurately and efficiently identified, and the method has the advantages of high accuracy and high efficiency. The accuracy, the real-time performance and the automation degree of pollen monitoring are remarkably improved.

The invention provides a pollen detection method and system based on a pollen scanning film, and relates to the technical field of image processing, and the method comprises the steps: obtaining the pollen scanning film; based on an rtmdet network, establishing a pollen detection model; inputting the pollen scanning film into a pollen detection model, and outputting coordinates, width and height of target pollen; determining a target pollen cutting image according to the coordinates, width and height of the target pollen; establishing a pollen filtering model; inputting the cut image into a pollen filtering model, and outputting coordinates, width and height of clear pollen; determining a clear pollen cutting image according to the coordinates, width and height of the clear pollen; establishing a family classification model; inputting the clear pollen cutting image into a category classification model, and determining the category of the clear pollen; and counting the pollen quantity of each family category and the total pollen quantity of the pollen scanning film to finish pollen detection. The pollen detection efficiency and accuracy can be improved.

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The invention discloses a method and a system for rapidly identifying a photo-thermo-sensitive sterile line of forage grass sorghum, and relates to the technical field of agricultural remote sensing. The method comprises the following specific steps: taking a suspected photo-thermo-sensitive sterile line as a material to be detected; acquiring hyperspectral data of the to-be-detected material after heading and before pollen formation by using a hyperspectral imaging technology; extracting identification characteristics related to the photo-thermo-sensitive sterile line of the forage grass sorghum from the hyperspectral data; and establishing an identification model based on the identification characteristics, and inputting a to-be-detected sample into the identification model for rapid identification of the photo-thermo-sensitive sterile line. The method is based on a hyperspectral imaging technology and is combined with a machine learning algorithm to realize rapid, lossless, visual and accurate identification of the photo-thermo-sensitive sterile line of the forage grass sorghum, and the method can greatly improve the identification efficiency and accuracy.

The invention discloses a pollen bioaerosol concentration monitoring system and monitoring method, and relates to the technical field of pollen bioaerosol concentration detection. The monitoring system comprises a power unit, a light source, a detector, a measuring cavity and a data processing unit, the power unit is used for outputting negative pressure power to enable air of a measured environment to enter the measuring cavity, and to-be-measured air is obtained; the air to be detected contains pollen particles; the light source is used for providing incident light for to-be-detected air; the detector is used for receiving an elastic scattering field intensity signal and a fluorescence scattering field intensity signal which are generated after incident light irradiates air to be detected, converting the elastic scattering field intensity signal into a first time domain electric pulse signal and converting the fluorescence scattering field intensity signal into a second time domain electric pulse signal; the data processing unit is electrically connected with the detector; the data processing unit is used for determining the number of pollen particles in the air to be detected according to the first time domain electric pulse signal and the second time domain electric pulse signal. According to the invention, the accuracy and real-time performance of detection can be improved.

The invention discloses application of a pear transcription factor PbrWHY2 gene in promoting growth of pear pollen tubes, and belongs to the technical field of plant genetic engineering. According to the invention, the gene PbrWHY2 is cloned from pollen of Dangshan pears by using a plant gene cloning technology, and the nucleotide sequence of the gene PbrWHY2 is shown as SEQ ID No.1; the amino acid sequence coded by the gene is SEQ ID No.2 in a sequence table. The PbrWHY2 can promote the growth of a pear pollen tube, the change of the expression of the PbrWHY2 is related to the change of ROS level and cellulose content at the top end of the pollen tube, and meanwhile, the PbrWHY2 also participates in a regulation mechanism of a self-incompatible reaction to regulate a downstream gene PbrAGP16 / PbrRALF6 / PbrGIS1 in a targeted manner. The pollen magnetic transfection technology is adopted to research the function of the gene in the pollen tube, and a wide application prospect is provided for improving the pollination efficiency.

The invention discloses a preparation method of a composition for dispelling effects of alcohol and protecting liver, and belongs to the technical field of traditional Chinese medicines. The liver protection composition is prepared from the following raw materials: gynostemma pentaphyllum powder, honeysuckle flower powder, hawthorn fruit powder, green tea powder, lotus leaf powder, cassia seed powder and folium sennae powder. The preparation method of the liver protection composition comprises the following steps: enzymolysis, fermentation and freeze-drying. According to the invention, through an extraction method of firstly performing enzymolysis and then performing fermentation, various different types of active effective components are reserved as far as possible. Tests prove that the finally obtained composition has a statistical therapeutic effect on alcoholic liver injury.

The invention relates to an autonomous pollination device and method for Amomum villosum, and the device comprises a mechanical dog which is arranged at the bottom of the pollination device and is used for controlling the moving direction and the moving route of the device; the docking station and the laser radar are arranged above the mechanical dog, the laser radar can be used for detecting obstacles, and the docking station can be used for expanding the mechanical arm; the mechanical arm is mounted on the docking station and is used for receiving the pollination signal and executing a pollination instruction; the tail end executor is connected to the tail end of the mechanical arm and used for being aligned with a target flower; the depth camera is connected to the end effector and used for collecting environment information and collecting an image of Amomum villosum; and the flexible clamping jaw is connected to the front of the depth camera and is used for pollinating the Amomum villosum detected by the depth camera. Efficient and accurate pollination is realized, the technical problems that pollen is difficult to fall on stigmas and self-pollination cannot be realized due to the fact that the floral organ structure of the amomum villosum is slightly higher than anther than the special stigmas are solved, and the pollination success rate is increased.

The utility model relates to the technical field of plant pollen collection, and discloses a rapid field apocynum venetum pollen collecting device which comprises a pollen scattering device, the pollen scattering device comprises a stamen inlet, a stamen processing pipe is welded to the bottom of the stamen inlet, a pollen scattering and spreading cavity is welded to the bottom of the stamen processing pipe, and the pollen scattering and spreading cavity is communicated with the stamen inlet. Four cavity wall attachment connecting strips are welded to the inner side of the pollen scattering and spreading cavity, a middle rotating shaft is welded to one ends of the four cavity wall attachment connecting strips, a wind driven fan is connected to the inner side of the middle rotating shaft through a rotating shaft, and a pollen scattering brush is welded to the top of the wind driven fan; an impurity filter screen is connected to the middle of the inner side of the pollen scattering and spreading cavity through a bolt, and the problems that according to an existing rapid field apocynum venetum pollen collecting device, pollen falls off through mechanical vibration or airflow blowing, and certain influences are caused to plants in actual use, such as flower bud damage and pollination influence are solved.

The invention relates to a constitutive promoter and application thereof. The nucleotide sequence of the constitutive promoter comprises SEQ ID NO: 35. The constitutive promoter disclosed by the invention almost shows activity in most tissues and many types of cells of a plant, especially in roots, stems, leaves, filaments, pollen, grains and spike-stalk apexes of the plant, and has a wide application prospect in the plant.

The invention belongs to the technical field of biology, and particularly relates to a Yu-grain rpl16 gene and application thereof, in particular to application of the rpl16 gene in cultivation of a rice sterile line. Overexpression of the Yu-grain rpl16 gene in rice can cause rice pollen abortion, so that rice male sterility is realized, the breeding period is shortened, the breeding efficiency is improved, and theoretical guidance is provided for culturing a new male sterility line.

The invention discloses a carrier plant system for preventing and treating greenhouse thrips. The carrier plant system comprises carrier plants, alternative plants and beneficial organisms, the carrier plant is ornamental pepper, the substitute food is pollen of the ornamental pepper, and the beneficial organism is predatory mites. After the predatory mites are inoculated in the flowering period of the ornamental pepper, the ornamental pepper is suspended and fixed on a greenhouse target crop, so that the harm can be effectively controlled in the early stage of the greenhouse thrips, the death phenomenon of the released predatory mites due to insufficient food is reduced, the thrips on the target crop are attracted to a carrier plant to a certain extent, natural enemies can search and prey conveniently, and the harm of the thrips is reduced. The action time of releasing the predatory mites is systematically prolonged, the predatory mites can establish populations easily, pests can be effectively controlled in the early stage of harm, the dosage of chemical pesticides is reduced, and the biological control efficiency of greenhouse thrip pests is improved.

The invention belongs to the technical field of functional food and health food, and particularly relates to a composition with skin whitening and brightening effects and a preparation method thereof. The composition comprises rice peptide, orange osmanthus pollen, white tomato powder, tremella polysaccharide, pomegranate concentrated powder and nicotinamide. Experimental results show that the composition reduces synthesis of melanin by inhibiting the activity of tyrosinase and achieves the effects of whitening and brightening the skin.

This invention describes a new method to generate cereal crop seed. The process involves the delivery of pollen of the male parent at will, as available either in a preserved pollen bank, or using real-time collection from male plants as they become available. Desired pollen is delivered to fertile females during the period when viable pollen from the females and locally proximal unrelated plants is not being released. The delivered male pollen is in such amounts and fortuitously timed that it preferentially pollinates the females. Such fortuitous timing may involve the intentional application of pollen to females a day or two prior to female parent pollen becoming viable, and / or during several periods wherein female parent pollen and / or other proximal plant pollen is not being shed.

The present invention relates to the field of environmental monitoring technology, and specifically discloses an atmospheric pollen online detection system, including a sampler, a transferor and an analyzer, wherein the sampler is used to collect pollen, the transferor is used to transfer the sampling carrier on the sampler to the analyzer, the analyzer is used to analyze the pollen conditions on the sampling carrier, the sampler includes an isolation cover and a sampling carrier placement table installed in the isolation cover, the isolation cover includes an upper part and a lower part, and also includes a push block that drives the upper part or the lower part to move, the push block is used to engage or separate the upper part with the lower part after moving, and an elastic member is connected between the push block and the isolation cover; and also includes a storage mechanism for storing the sampling carrier, the storage mechanism includes a storage plate, and the storage plate is provided with a plurality of glass slide storage positions. This scheme is used to solve the collision problem or the problem of poor sealing effect caused by the difficulty in adjusting the moving path of the isolation cover when it is closed in the prior art.

The present invention belongs to the technical field of plant pollination devices, and particularly relates to a handheld electric fruit tree pollinator and a pollination method. The handheld electric fruit tree pollinator includes: a depth camera (1), a lateral vibration motor (2), a lateral vibration motor cover (3), a solenoid valve (4), a solenoid valve cover (5), a sealing ring (6), a filter screen (7), a filter screen connecting piece (8), a fluff brush connecting piece (9), a fluff brush (10), a longitudinal vibration motor cover (11), a longitudinal vibration motor (12), a powder chamber cover (13), a powder chamber (14), an elastic rod (15), a screw (16), a multi-joint arm hand rod (17), and an intelligent controller (18); the present invention is convenient to carry and simple to operate; the solenoid valve is closed in the non-working state, reducing pollen waste; based on the pollination principle of insects (bees), the pollen is charged with static electricity through the fluff brush and high-frequency vibration, improving the pollination success rate and reducing the damage to the inflorescence.

The invention discloses a pollen monitoring method based on Fourier-wavelet transform fusion, and belongs to the technical field of environment monitoring, and the method specifically comprises the following steps: S1, carrying out pollen sampling through a sampler, and obtaining a hologram of a pollen sample; s2, performing numerical reconstruction on the obtained hologram, and simulating light propagation by using Fourier transform and a frequency domain transfer function to obtain complex amplitude distribution on a reconstruction plane; s3, performing two-dimensional wavelet decomposition on the reconstructed image, and calculating a wavelet coefficient variance; s4, performing multi-scale depth fusion reconstruction, selecting a wavelet coefficient with the maximum variance by traversing different reconstruction depths, and performing fusion to obtain an optimal reconstruction image; s5, performing post-processing on the reconstructed image; and S6, detecting particles on the processed image, extracting morphological characteristics, and carrying out pollen particle classification, statistics and visualization based on the characteristics. According to the method, the reproduction and identification accuracy of the pollen particles in the lensless digital holographic technology is remarkably improved.

The utility model provides an artificial pollination tool for breeding, which comprises a box body, a pollen storage box with a conical bottom is arranged on the box body, a discharge pipe is communicated with the bottom of the pollen storage box, a rotating shaft is arranged in the pollen storage box and the discharge pipe, an auger blade is arranged on the rotating shaft, a pipeline is arranged at the box body in a penetrating manner, and the auger blade is arranged on the pipeline. The pipeline is fixedly connected with the box body, the pipeline is communicated with the discharging pipe, an air pump is installed in the box body, an air outlet of the air pump is communicated with the pipeline through a hose, the part, located outside the box body, of the pipeline is sleeved with an adjusting pipe, and the adjusting pipe is in sliding connection with the pipeline. According to the artificial pollination device, the problems that the existing artificial pollination mostly adopts manual pollination, the operation is inconvenient, the coverage area of pollination is small, the pollen coverage is non-uniform, and the labor cost is relatively high are solved.

The invention provides an application of an OsINV2 gene in regulation and control of rice cold stress resistance. A nucleotide sequence of the OsINV2 is shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4. By revealing the key regulation and control effect of the vacuole sucrose invertase gene OsINV2 in rice low-temperature stress response, the molecular mechanism that the gene affects the cold resistance of plants by regulating the sugar metabolism balance in vacuoles is illustrated for the first time. The expression of the OsINV2 is regulated in a targeted manner through molecular marker-assisted breeding or a gene editing technology, so that the low-temperature survival rate in the seedling stage and the cold resistance in the booting stage of the rice can be remarkably improved, glumous flower degeneration and pollen abortion caused by low temperature are reduced, and meanwhile, the limitation of the cold resistance of varieties on geographical distribution is broken through; a theoretical support and a technical path are provided for breeding and adaptive popularization of cold-resistant rice varieties.

The invention relates to a constitutive promoter and application thereof. The nucleotide sequence of the constitutive promoter comprises SEQ ID NO: 39. The constitutive promoter disclosed by the invention almost shows activity in most tissues and many types of cells of a plant, especially in roots, stems, leaves, filaments, pollen, grains and spike-stalk apexes of the plant, and has a wide application prospect in the plant.

The invention relates to a constitutive promoter and application thereof. The nucleotide sequence of the constitutive promoter comprises SEQ ID NO: 34. The constitutive promoter disclosed by the invention almost shows activity in most tissues and many types of cells of a plant, especially in roots, stems, leaves, filaments, pollen, grains and spike-stalk apexes of the plant, and has a wide application prospect in the plant.