47 results about "Pollen tube" patented technology

The invention discloses a management method for improving cold resistance and drought resistance of macadamia nuts and increasing flowering quantity, which combines a four-stage dynamic management mode of soil improvement, cold resistance induction, drought resistance and tip control and flowering phase quality improvement, applies an organic fertilizer, a water-retaining agent and a composite slow-release fertilizer, induces root development, slowly releases the fertility of a chemical fertilizer, prolongs the action time of the chemical fertilizer, and improves the yield of the macadamia nuts. Brassinolide and potassium fulvate are compounded to activate cold-resistant gene expression of macadamia nut plants and improve cell membrane stability, so that cold resistance of the macadamia nut plants is improved, melatonin and methyl jasmonate perform double shoot control, an ABA signal channel of the plants is triggered, drought resistance of the plants is improved, flower bud differentiation is promoted, and growth of the macadamia nut plants is promoted. The synergistic effect of alginate oligosaccharide and gamma-aminobutyric acid enhances the elongation ability of a pollen tube, chemical agent stimulation is replaced by biological agent stimulation, the utilization rate of chemical agents is reduced, then the negative influence of the chemical agents on soil is reduced, and finally the fruit setting rate and the sustainable utilization of the soil are improved.

The invention discloses application of a brassica juncea gene BjuABCG42-A03 in regulating and controlling pollen germination and seed setting amount of brassica juncea, and relates to the technical field of plant genetic engineering, the amino acid sequence of the protein BjuABCG42-A03 is as shown in SEQ ID No.1, and meanwhile, the invention provides a method for cultivating a transgenic plant capable of improving the pollen germination rate or seed setting amount. The method comprises the following steps: constructing a recombinant plant expression vector containing a gene for coding protein BjuABCG42-A03; the constructed recombinant plant expression vector is transformed into agrobacterium and then transformed into a plant or a plant cell, and the gene of the coded protein BjuABCG42-A03 is over-expressed in the plant, so that the pollen germination rate or the seed setting amount of the plant is increased. Based on gene information of arabidopsis ABCG42 (At4g15233), a BjuABCG42-A03 gene is homologous cloned in a mustard genome, in-vivo and in-vitro germination experiments show that the BjuABCG42-A03 gene affects the elongation length and seed bearing quantity of a pollen tube, and a research direction is provided for solving the problem of low pollen fertility or low seed setting quantity of mustard subsequently.

The application discloses a kind of blending fertilizer raw material potash fertilizer and its preparation method, including the following weight parts of raw materials: 980 parts of potassium sulfate powder, 3 parts of zinc sulfate monohydrate, 10 parts of magnesium sulfate heptahydrate, 2 parts of sodium octaborate tetrahydrate, 1 part of DA-7, 4 parts of alginic polysaccharide.Seven water magnesium sulfate replenishes magnesium element, promotes the synthesis of carbohydrates, fats and proteins, is essential to maintain the green and healthy growth of crop leaves, and is conducive to improving the overall growth vigor and yield of crops.Four water sodium octaborate provides boron element, can promote pollen germination and pollen tube elongation, improve the flowering rate of crops, reduce the phenomenon of flower and fruit drop, DA-7 can promote plant cell division and elongation, improve the photosynthetic efficiency of plants, enhance the stress resistance and nutrient absorption capacity of plants, make crops grow more robustly, and improve fertilizer utilization.

The application belongs to the technical field of plant genetic engineering, and discloses a functional gene PbCOB.A.1 and application thereof. The in-vitro expressed recombinant protein PbCOB.A.1 can promote pollen tube growth of Rosaceae fruit trees, improve pollination efficiency, and expand the regulation mechanism of non-S factors participating in self-incompatibility reaction in pears. The mechanism of pear pollen tube elongation is researched by using magnetic transfection technology, so that the labor cost can be greatly reduced, and a theoretical basis is provided for pear breeding work.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses a nitrate ion transport channel gene PbrSLAH3b and application of the nitrate ion transport channel gene PbrSLAH3b in promoting growth of pear pollen tubules. The method comprises the following steps: extracting total RNA (Ribonucleic Acid) of Dangshan pears from pollen of Dangshan pears; carrying out reverse transcription on the RNA to obtain cDNA (complementary deoxyribonucleic acid); and by taking the cDNA as a template, carrying out amplification to obtain the pear pollen tube nitrate transport channel gene PbrSLAH3b, the PbrSLAH3b gene provided by the invention belongs to a slow anion channel SLAC / SLAH family member, the PbrSLAH3b is remarkably up-regulated in expression in the early growth stage of a pollen tube, and the PbrSLAH3b is positioned in a plasma membrane. The invention verifies that PbrSLAH3b can form a functional anion channel with NO3 <-> selectivity under the condition of not depending on proton gradient or phosphorylation state, and the PbrSLAH3b has relatively strong selectivity on NO3 <->. Therefore, the PbrSLAH3b gene can mediate the absorption and transport of the pollen tube to NO3 <-> and maintain the polarity growth directivity of the pollen tube, and has important application value for revealing the ion regulation mechanism of the growth of the fruit tree pollen tube and improving the reproductive development of the fruit tree.

The application discloses application of a CCHC type zinc finger protein PbrZFP719 gene in promoting pear pollen tube growth and belongs to the technical field of plant genetic engineering. The application clones the gene PbrZFP719 from 'yellow flower pear' pollen by using a plant gene cloning technique, the nucleotide sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence coded by the gene is shown in the sequence table SEQ ID No. 2. Research shows that the PbrZFP719 gene can promote pear pollen tube growth, the change of PbrZFP719 gene expression is related to the change of ROS level and cellulose content at the top of the pollen tube, and meanwhile, the PbrZFP719 gene also participates in the regulation mechanism of self-incompatibility reaction. The pollen magnetic transfection technology is used to study the function of the gene in the pollen tube, and provides a wide application prospect for improving pollination efficiency.

This invention belongs to the field of vegetable cultivation technology, specifically relating to a treatment method for enhancing the low-temperature resistance of tomato anthers and its application. The treatment method for enhancing the low-temperature resistance of tomato anthers includes the following steps: spraying with methyl jasmonate during the pre-opening stage of tomato flowers in a low-temperature environment; the low-temperature environment refers to an ambient temperature below 15℃. This treatment method can effectively alleviate the decline in pollen viability, reduced pollen tube germination rate, and adverse effects on fruit phenotype caused by low temperatures, thereby significantly mitigating the decrease in tomato fruit set rate caused by low-temperature injury.

The application discloses a fruit tree load intelligent decision-making method based on pollen tube behavior and carbon accumulation balance, and belongs to the field of fruit tree growth management. The method collects physiological data such as fruit tree pollen tube growth and leaf photosynthesis in real time through multiple sensors, combines environmental factors and carbon balance parameters, constructs a multi-source data fusion model, and realizes fine perception and dynamic optimization and regulation and control of fruit load under different growth states of fruit trees. The system introduces an abnormal data detection and self-adaptive evolution mechanism to improve the adaptability and robustness of the model to complex field environments and various varieties. The application can effectively improve the yield and quality of fruit trees, optimize the utilization efficiency of carbon resources, and promote the intelligent and fine management of orchards.

The invention relates to the technical field of forest tree genetic breeding and plant reproduction regulation and control, and particularly discloses a black poplar and white poplar distant hybridization obstacle breakthrough method based on sodium benzoate treatment. According to the method, female flowers of black poplar are taken as a treatment object, a sodium benzoate aqueous solution with the final concentration of 5-15 mM (preferably 10 mM) is used for carrying out dipping treatment on the female flowers, and then poplar pollen pollination is carried out. After pollination, fixing, softening and aniline blue dyeing are carried out, and pollen germination and pollen tube growth conditions are observed. Experimental results show that the method can significantly promote the growth of the poplar pollen on the black poplar stigma, improve the ability of a pollen tube to penetrate through the stigma, and realize fertilization and hybrid embryo formation, thereby effectively breaking through the distant hybridization obstacle between different branches of the poplar. The method disclosed by the invention is simple and convenient to operate, low in cost and good in repeatability, provides an efficient and feasible technical means for distant hybridization breeding of poplars, and has a good popularization and application prospect.

The present invention discloses an application of a rice THION22 small peptide protein in breaking the reproductive isolation between indica and japonica subspecies of rice, belonging to the field of biotechnology. The present invention breaks the pre-zygotic reproductive isolation of the indica and japonica subspecies of rice by applying the rice THION22 small peptide protein in vitro during the indica-japonica hybridization process. The application includes the rice THION22 small peptide protein promoting the growth of japonica rice pollen tubes in the pistil of the indica rice variety, and the rice THION22 small peptide promoting the acquisition of indica-japonica hybrid F1 hybrid seeds. The present invention effectively promotes the hybridization of japonica rice pollen with some indica rice varieties, promotes the growth of japonica rice pollen tubes, produces more F1 hybrid seeds, improves the efficiency of indica-japonica hybridization breeding, and has important application value for rice hybridization breeding and heterosis practice.

The invention provides application of citrus S-adenosylmethionine synthetase in improving self-incompatibility, and belongs to the technical field of biological genetic engineering, and the gene sequence of the citrus S-adenosylmethionine synthetase is shown as SEQ ID: 1. Experiments prove that citrus S-adenosylmethionine synthetase directly interacts with S-RNase to regulate and control a methionine metabolic pathway so as to influence the growth of a pollen tube; the invention discloses a dual regulation and control effect of the citrus S-adenosylmethionine synthetase in a self-incompatible reaction, and provides a new target for improving the self-compatibility of crops. According to the method, the citrus S-adenosylmethionine synthetase is added into pollen culture or methionine is sprayed onto styles, so that the growth of pollen tubes is improved, the self-incompatibility of the citrus is further improved, a new strategy is provided for improving the self-incompatibility of crops, and the problems of low fruit setting rate, high artificial pollination cost and the like caused by the self-incompatibility are hopefully solved.

The invention relates to the technical field of durian planting, and particularly provides a regulation and control method for promoting and protecting flowers of durian and a medicament. Through comprehensive measures such as environment regulation and control, nutrition management, medicament treatment and flowering period management, flowering and flower protection of the durian are effectively promoted, and the fruit setting rate is increased. Specifically, by controlling the drought period, adjusting the environmental factors such as illumination and humidity, and combining the application of the fish protein fermentation broth and the flower-promoting and flower-protecting agent for the durian, suitable conditions are provided for the growth and flowering of the durian. Meanwhile, monopotassium phosphate, gibberellic acid, calcium amino acid chelate and a boric acid solution are sprayed in the flowering period, and flower bud differentiation and development and pollen tube growth and fertilization are further promoted. The method and the agent are low in cost, easy and convenient to operate, environmentally friendly, safe and suitable for large-scale application and popularization, and the yield and economic benefits of the durian can be remarkably improved.

The application discloses application of a rapid alkalization factor family gene PbrRALF6 in promoting pear pollen tube growth and belongs to the technical field of plant genetic engineering. The application clones the gene PbrRALF6 from 'Dangshan quxi pear' pollen by using plant gene cloning technology, the nucleotide sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence coded by the gene is shown in the sequence table SEQ ID No. 2. Research shows that PbrRALF6 can promote pear pollen tube growth, the change of PbrRALF6 expression is related to the change of the ROS level at the top of the pollen tube, and meanwhile, PbrRALF6 also participates in the regulation mechanism of self-incompatibility reaction. The pollen magnetic transfection technology is used to study the function of the gene in the pollen tube, thereby providing wide application prospects for improving pollination efficiency.

This invention provides the application of citrus S-adenosylmethionine synthase in improving self-incompatibility, belonging to the field of biogenetic engineering technology. The gene sequence of citrus S-adenosylmethionine synthase is shown in SEQ ID:1. This invention experimentally demonstrates that citrus S-adenosylmethionine synthase affects pollen tube growth by directly interacting with S-RNase and regulating the methionine metabolic pathway. This invention reveals the dual regulatory role of citrus S-adenosylmethionine synthase in self-incompatibility responses, providing a new target for improving crop self-compatibility. This invention improves pollen tube growth by adding citrus S-adenosylmethionine synthase to pollen culture or spraying methionine onto the style, thereby improving citrus self-incompatibility. This provides a new strategy for improving crop self-compatibility and is expected to solve problems such as low fruit set rate and high artificial pollination costs caused by self-incompatibility.

The invention relates to the technical field of plant genetic engineering, and particularly discloses application of an alfalfa MsWRKY49 gene to regulation and control of elongation of a pollen tube under low boron stress. Researches find that the MsWRKY49 gene responds to low-boron stress through an active oxygen (ROS) signal channel, and the expression quantity of the MsWRKY49 gene is remarkably increased under a low-boron condition. The construction of an MsWRKY49 overexpression strain and a gene editing mutant proves that the gene has a negative regulation effect on the elongation of a pollen tube: the length of the pollen tube of an overexpression plant is shortened under a low-boron condition, the rupture rate is increased, and the mutant presents an opposite phenotype. According to the invention, the function of the MsWRKY49-ROS regulation module in boron signal transduction is disclosed for the first time, and new gene resources and molecular breeding strategies are provided for genetic improvement of crops in boron-deficient regions.

The invention discloses a method for determining pollination affinity among camellia oleifera varieties based on pollen tube in-situ germination, and belongs to the technical field of agricultural planting. The method comprises the following steps: collecting pistil samples which are pollinated for 48-72 hours, fixing and slicing, and quantifying the pollination affinity by adopting fluorescence microscopic observation in combination with a five-level weighted scoring system (1 score of stigma, 2 scores of upper part of stylus, 3 scores of lower part of stylus, 4 scores of ovary cavity and 5 scores of ovule). Compared with a traditional fruit setting rate method, the method has the advantages that the detection period is shortened from 1-3 months to 3 days, the affinity of different pollination variety combinations can be distinguished more accurately, and the method is particularly suitable for variety matching selection in camellia oleifera cross breeding.

The pollen activation solution (1) contains an aqueous solution containing a substance for activating the germination of pollen and the elongation of a pollen tube, and a powdered carbon-constituting substance added to the aqueous solution. The carbon-constituting material is not activated. For example, sucrose, boric acid, magnesium sulfate heptahydrate, calcium chloride, potassium chloride, gelatin or the like can be used as a substance for activating the germination of pollen and the elongation of pollen tubes. For example, a bamboo leaf charcoal powder obtained by pulverizing bamboo leaf charcoal can be used as the powdered carbon constituent material.

A pollen activation solution (1) includes: an aqueous solution containing a substance that activates pollen germination and pollen tube elongation; and a powdered carbon constituent added to the aqueous solution. The carbon constituent has not been subjected to an activation treatment. Examples of the substance that activates pollen germination and pollen tube elongation, which can be used herein, include sucrose, boric acid, magnesium sulfate heptahydrate, calcium chloride, potassium chloride, and gelatin. An example of the powdered carbon constituent that can be used herein is bamboo leaf charcoal powder made by pulverizing bamboo leaf charcoal.

The invention belongs to the technical field of agriculture and biology, discloses application of arabidopsis thaliana GALT4 in regulation and control of pollen development, and verifies that the arabidopsis thaliana GALT4 gene is a plant male fertility key regulation and control gene with high pollen specificity expression, and the CDS sequence is shown as SEQ ID NO.1 for the first time. Polarity elongation of the pollen tube is maintained by regulating and controlling spatial and temporal distribution of methyl esterified pectin in the pollen tube. Knockout or silencing of the homologous gene in a plant can lead to reduction of pollen viability, abnormal development of pollen cell nucleus, malformation of pollen morphology, slowing of pollen germination rate and hindering of pollen tube polarity elongation. The invention provides a brand new molecular target for plant pollen morphological development and pollen tube growth regulation and control, can be used for fertility regulation and control and hybrid seed production optimization of cruciferous crops, and has important theoretical research value and industrial application prospect.

The invention discloses a method for improving the natural pollination success rate of Lingyun Niuxin plums, and belongs to the technical field of fruit tree cultivation. The method comprises the following steps: spraying a pollen activity enhancer compounded by compound sodium nitrophenolate, borax, monopotassium phosphate and zinc sulfate at the initial flowering stage of pollinizers so as to remarkably improve the pollen activity and timeliness; spraying superfine water mist by adopting a high-pressure mist spraying machine in the early morning of the full-bloom stage so as to optimize the pollination microenvironment, prolong the pollination ability of stigmas and promote the growth of pollen tubes; in a key period after blossom fall, compound fertilizer is immediately dressed, and a foliar nutrient solution containing urea and calcium chloride is sprayed to guarantee young fruit development and prevent physiological fruit drop. Through the synergistic effect of the systematic technical scheme, the defect that traditional natural pollination depends on the day to eat is thoroughly overcome, on the premise that artificial pollination is not conducted, the fruit setting rate can be remarkably increased to 68.5% or above, meanwhile, the fruit cracking rate is effectively reduced, unification of high yield and high quality is achieved, the core bottleneck problem of Lingyun Niuxin plum industry development is solved, and the market prospect is wide. The economic benefit is obvious.

The invention provides a cross breeding method for olea europaea, which belongs to the technical field of plant breeding, and ensures sperm motility by limiting the composition of premixed pollen; by coating conductive gel on a stigma and combining with a 20-40kHz ultrasonic field to generate a directional acoustic streaming effect, the growth speed of a pollen tube is increased by 2.1 times, and the pollen tube is accurately guided to an embryo sac to improve the fertilization efficiency; then smearing a sealing agent on the fertilized stigmas to improve the occurrence probability of the hybrid seeds; finally, female parents with infertility and high resistance and male parents with excellent characters are screened, and the occurrence probability of the high-resistance hybrid olive seeds is further increased. According to the hybridization method disclosed by the invention, the culture period of high-quality hybrid varieties is greatly shortened, and new varieties with high resistance can be obtained in a relatively short time.

The application discloses application of a pear transcription factor PbrWHY2 gene in promoting pear pollen tube growth and belongs to the technical field of plant genetic engineering. The application clones a gene PbrWHY2 from 'Dangshan pear' pollen by using a plant gene cloning technique, the nucleotide sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence coded by the gene is shown in the sequence table SEQ ID No. 2. The PbrWHY2 can promote pear pollen tube growth, the change of PbrWHY2 expression is related to the change of ROS level and cellulose content at the top of the pollen tube, meanwhile, the PbrWHY2 also participates in the regulation mechanism of self-incompatibility reaction, and targets to regulate downstream genes PbrAGP16 / PbrRALF6 / PbrGIS1. The pollen magnetic transfection technology is used to study the function of the gene in the pollen tube, and provides a wide application prospect for improving pollination efficiency.

The invention discloses a fruit tree load intelligent decision-making method based on pollen tube behaviors and carbon accumulation balance, and belongs to the field of fruit tree growth management. According to the method, physiological data of fruit tree pollen tube growth, leaf photosynthesis and the like are collected in real time through multiple sensors, a multi-source data fusion model is constructed in combination with environmental factors and carbon balance parameters, and fine sensing and dynamic optimization regulation and control of the flower and fruit loading capacity of the fruit trees in different growth states are achieved. Abnormal data detection and a self-adaptive evolution mechanism are introduced into the system, so that the adaptability and robustness of the model to a complex field environment and various varieties are improved. By applying the method, the fruit tree yield and fruit quality can be effectively improved, the utilization efficiency of carbon resources is optimized, and intelligent and refined management of an orchard is promoted.

The invention discloses a pollination method of prunus armeniaca varieties, and relates to the technical field of pollination of prunus armeniaca varieties, and the pollination method comprises the following steps: carrying out gene analysis on a main cultivated prunus armeniaca variety and candidate pollination varieties, screening out a pollination variety similar to the main cultivated variety, artificially pollinating pollen of the screened pollination variety to stigmas of the main cultivated variety under a field test condition, and carrying out pollination on the stigmas of the main cultivated variety to obtain the prunus armeniaca variety. The growth condition of the pollen tube and the final fruit setting performance are observed; the method comprises the following steps: preparing a mucus substrate by taking natural vegetable gelatin as a main substrate and simulating physicochemical properties of natural secretions of apricot and plum stigmas, adding plant pigments into the prepared mucus substrate as a visual indicator, and adding boric acid as a pollen germination accelerator to form pollination prefabricated mucus; the prepared pollination prefabricated mucus is subpackaged and then placed in a low-temperature environment to be sealed and stored, and the activity of the mucus is kept; in the plum full-bloom stage, a composite pollination mode combining artificial pollination and bee supplementary pollination is adopted; and an environmental adaptability regulation and control system is adopted to monitor flowering phase environmental parameters in real time.

The invention discloses a sugarcane hybridization auxiliary device and a use method thereof. The sugarcane hybridization auxiliary device is characterized in that a working frame is arranged in a sugarcane hybridization shed; the spica fixing module is provided with a mechanical arm and a clamping jaw, the clamping jaw is connected with the mechanical arm, and a pressure sensor is arranged in an area where the clamping jaw clamps a sugarcane spica stem; the castration module comprises a visual identification module and a cutter mechanism, the visual identification module is mounted on the cutter mechanism, and the cutter mechanism is mounted on the mechanical arm; the pollen management module is provided with a pollen box and a pollination pipeline, the pollen box is mounted at the top of the working frame, one end of the pollination pipeline is connected with the pollen box, and the other end of the pollination pipeline faces sugarcane flower spikes; the isolation hood module is movably connected with the working frame, and the isolation hood can cover the outer side of the sugarcane spica or be far away from the sugarcane spica; the control module controls the above structures to operate. According to the device and the using method thereof, clamping, recognition, castration, quantitative pollination and isolation are integrated, manual operation is greatly reduced, the automation degree is high, and the device and the using method belong to the technical field of agricultural machinery.

This invention belongs to the field of plant biotechnology, specifically disclosing a rice THION22 gene and its application in regulating rice pollen tube growth. Experiments show that the THION22 gene can regulate the growth of rice pollen tubes in the pistil, thereby affecting the seed setting rate and ultimately rice yield. This research on the regulation of rice pollen tube growth is of great significance and has application value for rice breeding.

The application belongs to the technical field of plant transgenics, and particularly relates to a nano-magnetic-bead-mediated pollen tube channel transgenic method of Pennisetum. The method comprises the following steps: magnetic bead and plasmid DNA complexing, magnetic bead-DNA complex and pollen culture solution complexing, magnetic transfection, and pollination. Compared with a traditional transformation mode, the nano-magnetic-bead-mediated transformation method does not need to pass through tissue culture, the transformation condition is more moderate, the time is shorter, and the method is a high-efficiency and rapid transformation mode, which has great guiding significance for the genetic breeding work of the Pennisetum. A large amount of seeds can be obtained through artificial pollination, and more materials are provided for the screening of subsequent transgenic plants. The method is simple in operation, saves resources, and has a synergistic effect between the operation steps, and excellent effects are achieved.

The application discloses application of a pear PbrGIS1 gene in promoting growth of a pear pollen tube and belongs to the technical field of plant genetic engineering. The application clones a gene PbrGIS1 from pollen of 'Dangshan Quli' pear by using a plant gene cloning technique, the nucleotide sequence of the gene PbrGIS1 is shown in SEQ ID No. 1, and the amino acid sequence coded by the gene is shown in the sequence table SEQ ID No. 2. Research shows that PbrGIS1 can promote growth of the pear pollen tube, the change in expression of PbrGIS1 is related to the change in a ROS level and a cellulose content at a top end of the pollen tube, and meanwhile, PbrGIS1 also participates in a regulation mechanism of a self-incompatibility reaction. The pollen magnetic transfection technique is used to study the function of the gene in the pollen tube, and provides a wide application prospect for improving pollination efficiency.

The invention discloses a method for identifying self-cross affinity of jujube trees through in-vitro pollination and in-vitro culture, and belongs to the technical field of jujube tree breeding. The method comprises the following steps: taking male and female parents (hybrid) or single parent (selfing) non-cracked buds at a yellow bud stage, and sterilizing by using a composite disinfectant prepared from water, anhydrous alcohol, a wild jujube fruit extract, paenibacillus polymyxa powder, ascorbic acid and citric acid according to a mass ratio of 1: 9: 1: (3-5): (2-3): 1; inoculating the sterilized buds to an MS composite culture medium containing an MS culture medium, 0.4-0.6 g / L aniline acid peptide and 0.04-0.1 g / L branched chain amino acid; after the pollen and stigmas grow to be in a pollination state, pollinating the pollen to the stigmas of the female parents, continuously culturing for 6-10 hours, detecting the proportion of the pollen tube germinating and entering the stigmas by adopting a fluorescence microscope, and determining the transverse diameter of the ovary and calculating the expansion rate on the fifth day. According to the method, identification can be completed in about 5 days, the sterilization rate is larger than or equal to 85%, the pollen and stigma vitality can be effectively kept, and the method is suitable for high-throughput early screening of selfing or outcrossing affinity of jujube plants.

The present application relates to the technical field of plant tissue culture, in particular to a culture medium for in vitro germination of Hippophae rhamnoides pollen and a preparation method and application thereof. Each 100 mL of the culture medium comprises 0.5-1 g of agar, 15-25 g of sucrose, 0.0001-0.1 g of boric acid and 0.0003-0.001 g of calcium chloride. The present application promotes the germination of Hippophae rhamnoides pollen and the elongation of pollen tubes by adding agar, sucrose, boric acid and calcium chloride.