25 results about "Pollen tube" patented technology

The application belongs to the technical field of plant genetic engineering, and discloses a functional gene PbCOB.A.1 and application thereof. The in-vitro expressed recombinant protein PbCOB.A.1 can promote pollen tube growth of Rosaceae fruit trees, improve pollination efficiency, and expand the regulation mechanism of non-S factors participating in self-incompatibility reaction in pears. The mechanism of pear pollen tube elongation is researched by using magnetic transfection technology, so that the labor cost can be greatly reduced, and a theoretical basis is provided for pear breeding work.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses a nitrate ion transport channel gene PbrSLAH3b and application of the nitrate ion transport channel gene PbrSLAH3b in promoting growth of pear pollen tubules. The method comprises the following steps: extracting total RNA (Ribonucleic Acid) of Dangshan pears from pollen of Dangshan pears; carrying out reverse transcription on the RNA to obtain cDNA (complementary deoxyribonucleic acid); and by taking the cDNA as a template, carrying out amplification to obtain the pear pollen tube nitrate transport channel gene PbrSLAH3b, the PbrSLAH3b gene provided by the invention belongs to a slow anion channel SLAC / SLAH family member, the PbrSLAH3b is remarkably up-regulated in expression in the early growth stage of a pollen tube, and the PbrSLAH3b is positioned in a plasma membrane. The invention verifies that PbrSLAH3b can form a functional anion channel with NO3 <-> selectivity under the condition of not depending on proton gradient or phosphorylation state, and the PbrSLAH3b has relatively strong selectivity on NO3 <->. Therefore, the PbrSLAH3b gene can mediate the absorption and transport of the pollen tube to NO3 <-> and maintain the polarity growth directivity of the pollen tube, and has important application value for revealing the ion regulation mechanism of the growth of the fruit tree pollen tube and improving the reproductive development of the fruit tree.

This invention belongs to the field of vegetable cultivation technology, specifically relating to a treatment method for enhancing the low-temperature resistance of tomato anthers and its application. The treatment method for enhancing the low-temperature resistance of tomato anthers includes the following steps: spraying with methyl jasmonate during the pre-opening stage of tomato flowers in a low-temperature environment; the low-temperature environment refers to an ambient temperature below 15℃. This treatment method can effectively alleviate the decline in pollen viability, reduced pollen tube germination rate, and adverse effects on fruit phenotype caused by low temperatures, thereby significantly mitigating the decrease in tomato fruit set rate caused by low-temperature injury.

The application discloses a fruit tree load intelligent decision-making method based on pollen tube behavior and carbon accumulation balance, and belongs to the field of fruit tree growth management. The method collects physiological data such as fruit tree pollen tube growth and leaf photosynthesis in real time through multiple sensors, combines environmental factors and carbon balance parameters, constructs a multi-source data fusion model, and realizes fine perception and dynamic optimization and regulation and control of fruit load under different growth states of fruit trees. The system introduces an abnormal data detection and self-adaptive evolution mechanism to improve the adaptability and robustness of the model to complex field environments and various varieties. The application can effectively improve the yield and quality of fruit trees, optimize the utilization efficiency of carbon resources, and promote the intelligent and fine management of orchards.

The invention relates to the technical field of forest tree genetic breeding and plant reproduction regulation and control, and particularly discloses a black poplar and white poplar distant hybridization obstacle breakthrough method based on sodium benzoate treatment. According to the method, female flowers of black poplar are taken as a treatment object, a sodium benzoate aqueous solution with the final concentration of 5-15 mM (preferably 10 mM) is used for carrying out dipping treatment on the female flowers, and then poplar pollen pollination is carried out. After pollination, fixing, softening and aniline blue dyeing are carried out, and pollen germination and pollen tube growth conditions are observed. Experimental results show that the method can significantly promote the growth of the poplar pollen on the black poplar stigma, improve the ability of a pollen tube to penetrate through the stigma, and realize fertilization and hybrid embryo formation, thereby effectively breaking through the distant hybridization obstacle between different branches of the poplar. The method disclosed by the invention is simple and convenient to operate, low in cost and good in repeatability, provides an efficient and feasible technical means for distant hybridization breeding of poplars, and has a good popularization and application prospect.

The application discloses application of a rapid alkalization factor family gene PbrRALF6 in promoting pear pollen tube growth and belongs to the technical field of plant genetic engineering. The application clones the gene PbrRALF6 from 'Dangshan quxi pear' pollen by using plant gene cloning technology, the nucleotide sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence coded by the gene is shown in the sequence table SEQ ID No. 2. Research shows that PbrRALF6 can promote pear pollen tube growth, the change of PbrRALF6 expression is related to the change of the ROS level at the top of the pollen tube, and meanwhile, PbrRALF6 also participates in the regulation mechanism of self-incompatibility reaction. The pollen magnetic transfection technology is used to study the function of the gene in the pollen tube, thereby providing wide application prospects for improving pollination efficiency.

This invention provides the application of citrus S-adenosylmethionine synthase in improving self-incompatibility, belonging to the field of biogenetic engineering technology. The gene sequence of citrus S-adenosylmethionine synthase is shown in SEQ ID:1. This invention experimentally demonstrates that citrus S-adenosylmethionine synthase affects pollen tube growth by directly interacting with S-RNase and regulating the methionine metabolic pathway. This invention reveals the dual regulatory role of citrus S-adenosylmethionine synthase in self-incompatibility responses, providing a new target for improving crop self-compatibility. This invention improves pollen tube growth by adding citrus S-adenosylmethionine synthase to pollen culture or spraying methionine onto the style, thereby improving citrus self-incompatibility. This provides a new strategy for improving crop self-compatibility and is expected to solve problems such as low fruit set rate and high artificial pollination costs caused by self-incompatibility.

A pollen activation solution (1) includes: an aqueous solution containing a substance that activates pollen germination and pollen tube elongation; and a powdered carbon constituent added to the aqueous solution. The carbon constituent has not been subjected to an activation treatment. Examples of the substance that activates pollen germination and pollen tube elongation, which can be used herein, include sucrose, boric acid, magnesium sulfate heptahydrate, calcium chloride, potassium chloride, and gelatin. An example of the powdered carbon constituent that can be used herein is bamboo leaf charcoal powder made by pulverizing bamboo leaf charcoal.

The invention belongs to the technical field of agriculture and biology, discloses application of arabidopsis thaliana GALT4 in regulation and control of pollen development, and verifies that the arabidopsis thaliana GALT4 gene is a plant male fertility key regulation and control gene with high pollen specificity expression, and the CDS sequence is shown as SEQ ID NO.1 for the first time. Polarity elongation of the pollen tube is maintained by regulating and controlling spatial and temporal distribution of methyl esterified pectin in the pollen tube. Knockout or silencing of the homologous gene in a plant can lead to reduction of pollen viability, abnormal development of pollen cell nucleus, malformation of pollen morphology, slowing of pollen germination rate and hindering of pollen tube polarity elongation. The invention provides a brand new molecular target for plant pollen morphological development and pollen tube growth regulation and control, can be used for fertility regulation and control and hybrid seed production optimization of cruciferous crops, and has important theoretical research value and industrial application prospect.

The invention provides a cross breeding method for olea europaea, which belongs to the technical field of plant breeding, and ensures sperm motility by limiting the composition of premixed pollen; by coating conductive gel on a stigma and combining with a 20-40kHz ultrasonic field to generate a directional acoustic streaming effect, the growth speed of a pollen tube is increased by 2.1 times, and the pollen tube is accurately guided to an embryo sac to improve the fertilization efficiency; then smearing a sealing agent on the fertilized stigmas to improve the occurrence probability of the hybrid seeds; finally, female parents with infertility and high resistance and male parents with excellent characters are screened, and the occurrence probability of the high-resistance hybrid olive seeds is further increased. According to the hybridization method disclosed by the invention, the culture period of high-quality hybrid varieties is greatly shortened, and new varieties with high resistance can be obtained in a relatively short time.

The invention discloses a fruit tree load intelligent decision-making method based on pollen tube behaviors and carbon accumulation balance, and belongs to the field of fruit tree growth management. According to the method, physiological data of fruit tree pollen tube growth, leaf photosynthesis and the like are collected in real time through multiple sensors, a multi-source data fusion model is constructed in combination with environmental factors and carbon balance parameters, and fine sensing and dynamic optimization regulation and control of the flower and fruit loading capacity of the fruit trees in different growth states are achieved. Abnormal data detection and a self-adaptive evolution mechanism are introduced into the system, so that the adaptability and robustness of the model to a complex field environment and various varieties are improved. By applying the method, the fruit tree yield and fruit quality can be effectively improved, the utilization efficiency of carbon resources is optimized, and intelligent and refined management of an orchard is promoted.

This invention belongs to the field of plant biotechnology, specifically disclosing a rice THION22 gene and its application in regulating rice pollen tube growth. Experiments show that the THION22 gene can regulate the growth of rice pollen tubes in the pistil, thereby affecting the seed setting rate and ultimately rice yield. This research on the regulation of rice pollen tube growth is of great significance and has application value for rice breeding.

The application belongs to the technical field of plant transgenics, and particularly relates to a nano-magnetic-bead-mediated pollen tube channel transgenic method of Pennisetum. The method comprises the following steps: magnetic bead and plasmid DNA complexing, magnetic bead-DNA complex and pollen culture solution complexing, magnetic transfection, and pollination. Compared with a traditional transformation mode, the nano-magnetic-bead-mediated transformation method does not need to pass through tissue culture, the transformation condition is more moderate, the time is shorter, and the method is a high-efficiency and rapid transformation mode, which has great guiding significance for the genetic breeding work of the Pennisetum. A large amount of seeds can be obtained through artificial pollination, and more materials are provided for the screening of subsequent transgenic plants. The method is simple in operation, saves resources, and has a synergistic effect between the operation steps, and excellent effects are achieved.

The invention discloses a method for identifying self-cross affinity of jujube trees through in-vitro pollination and in-vitro culture, and belongs to the technical field of jujube tree breeding. The method comprises the following steps: taking male and female parents (hybrid) or single parent (selfing) non-cracked buds at a yellow bud stage, and sterilizing by using a composite disinfectant prepared from water, anhydrous alcohol, a wild jujube fruit extract, paenibacillus polymyxa powder, ascorbic acid and citric acid according to a mass ratio of 1: 9: 1: (3-5): (2-3): 1; inoculating the sterilized buds to an MS composite culture medium containing an MS culture medium, 0.4-0.6 g / L aniline acid peptide and 0.04-0.1 g / L branched chain amino acid; after the pollen and stigmas grow to be in a pollination state, pollinating the pollen to the stigmas of the female parents, continuously culturing for 6-10 hours, detecting the proportion of the pollen tube germinating and entering the stigmas by adopting a fluorescence microscope, and determining the transverse diameter of the ovary and calculating the expansion rate on the fifth day. According to the method, identification can be completed in about 5 days, the sterilization rate is larger than or equal to 85%, the pollen and stigma vitality can be effectively kept, and the method is suitable for high-throughput early screening of selfing or outcrossing affinity of jujube plants.

The present application relates to the technical field of plant tissue culture, in particular to a culture medium for in vitro germination of Hippophae rhamnoides pollen and a preparation method and application thereof. Each 100 mL of the culture medium comprises 0.5-1 g of agar, 15-25 g of sucrose, 0.0001-0.1 g of boric acid and 0.0003-0.001 g of calcium chloride. The present application promotes the germination of Hippophae rhamnoides pollen and the elongation of pollen tubes by adding agar, sucrose, boric acid and calcium chloride.

This invention relates to the field of plant microscopy, specifically a method for constructing a pollen library and measuring pollen species and germination rates using three microscopes simultaneously. Anthers or buds of known flowering plants in their flowering period are collected, and morphological and size data of the pollen are obtained using a bright-field microscope, a fluorescence microscope, and a scanning electron microscope, respectively, to form a pollen reference database. The stigmas of the plants to be tested are collected, and after clearing and staining, the morphological and size data of the pollen on the stigmas of the plants to be tested are obtained under a bright-field microscope and a fluorescence microscope, respectively. Based on the pollen reference database, the known flowering plants corresponding to the pollen attached to the stigmas of the plants to be tested are identified, and the presence of pollen tubes is observed using a fluorescence microscope. The germination rate of pollen from known flowering plants on the stigmas of the plants to be tested is then statistically analyzed. This method achieves simultaneous pollen species identification, germination rate statistics, and pollen tube growth observation.

The invention discloses a method for improving the germination rate of pear pollen by using DIECA. The method comprises the following steps: mixing pollen with a pear pollen liquid culture medium containing DIECA or an agricultural pollination liquid, and then carrying out pollen culture or pollination, or after the anther in the big bud stage is dried and looses powder, the pollen is sown on the pollen solid culture medium, then the pear pollen liquid culture medium containing DIECA is added, and the pear pollen liquid culture medium is cultured in a dark place. Research finds that the germination rate of pear pollen treated by DIECA is obviously increased compared with that of a contrast, and pollen tubes can grow well. The pollen germination time is short, and the application prospect is relatively good. The method has the advantages of simple operation steps, less loss, short time consumption and the like, reagents used in the formula do not contain dangerous chemicals, are low in price and easy to obtain, solution preparation is extremely simple and convenient, the pH value does not need to be additionally adjusted, and the purposes of improving the germination rate, ensuring the germination quality and saving cost and increasing income can be achieved while labor and time are not consumed.

The utility model is suitable for the technical field of plant vacuum infection, and provides an industrial cannabis sativa L. pollen tube channel genetic transformation vacuum device which comprises a vacuum tank used for providing an industrial cannabis sativa L. infection environment. The acrylic cover plate is detachably mounted at the top of the vacuum tank through nuts and bolts and is used for sealing the vacuum tank; the sealing rubber ring is arranged between the vacuum tank and the acrylic cover plate and is used for increasing sealing; the pressure gauge is mounted at the top of the acrylic cover plate and is used for monitoring the air pressure in the vacuum tank; and the air taps are fixed at the top of the acrylic cover plate, are respectively positioned on two sides of the pressure gauge and are used for exhausting air and feeding air. According to the industrial cannabis sativa pollen tube channel genetic transformation vacuum device provided by the scheme, the whole industrial cannabis sativa plant is inverted for dip dyeing, and the dip dyeing effect is effectively improved.

Rising global temperatures negatively impact crop yields by inhibiting seed and fruit production, primarily due to the vulnerability of pollen tube (PT) delivery of sperm cells to ovules under heat stress (HS). Two in vivo assays were developed to investigate how various HS conditions affect pollen-pistil interactions in Arabidopsis, revealing that HS impairs multiple stages of PT growth: pollen germination, growth in the transmitting tract, emergence from the transmitting tract, and ovule targeting, and seed formation. Increased temperatures progressively halted PT growth earlier in its journey toward the ovules. This disruption of pollen-pistil interactions may contribute to reduced crop yields under HS. Additionally described are expression cassettes designed to generate heat-tolerant plants by overexpressing LLG, offering a potential solution to mitigate the adverse effects of heat stress on crop production.

The application relates to the technical field of plant pollen extraction, and discloses a pollen extraction device for fruit tree pollination, which comprises a screening machine, a conveying belt assembly and a screening assembly which are sequentially connected, the conveying belt assembly comprises a conveying belt mounting frame, a sealing cover is arranged on the conveying belt mounting frame, the device further comprises a pre-drying assembly, the pre-drying assembly comprises a negative pressure exhaust fan and a drying assembly, and the negative pressure exhaust fan and the drying assembly are connected through a conveying pipe; the screening assembly comprises a drying separator and a fine selection separator, and further comprises a variable frequency fan; the variable frequency fan is connected with the drying separator and the fine selection separator through a pollen pipe, the pre-drying assembly installed on the conveying belt assembly removes dust in the air while preheating the flower buds, the flower buds are broken through the collision plate of the drying separator, and the air in the drying separator is extracted and dehumidified while the flower buds are collided.

The present invention relates to a method for producing male sterility and male sterile rice produced therefrom. According to the present invention, when the Os05g20150 gene is mutated and its expression and function are suppressed, pollen germination and pollen tube elongation are reduced, thereby causing male sterility. Therefore, the method for producing a male sterile line through the regulation of the activity of the Os05g20150 gene according to the present invention and the male sterile line produced therefrom are intended to effectively improve existing breeding methods. Since they can be used stably with almost no environmental influence, they can contribute significantly to the development of the rice breeding industry.

The application relates to the technical field of tropical fruit tree cultivation, and discloses application of L-serine in improving the pollination of mango pistil heads and / or the fruit setting rate of mangoes under low-temperature stress conditions, a composition and a method for promoting the fruit setting rate of mangoes. The application provides a new use of L-serine and a complexing agent thereof in improving the pollen vitality, the pollination of mango pistil heads and the fruit setting of mangoes under low-temperature stress conditions. Gamma-aminobutyric acid (GABA) and L-serine are complexed in a composition containing 100-300 umol of gamma-aminobutyric acid and 1-5 g of L-serine per liter, the composition is sprayed on plants in the initial flowering period, the composition has the functions of promoting the pollen germination, pollen tube elongation and pollination of mangoes, and the fruit setting rate is improved, thereby providing technical support for solving the unstable yield problem in the industry.

The present application relates to a kind of statistical camellia pollen germination rate and germination length method and system, method includes: S1, collection camellia pollen and in vitro culture, obtain the micrograph of camellia pollen tube, the micrograph is divided into training image and to be measured image;S2, different marking mode is handled to training image, obtain the detection label for statistics germination rate and the segmentation label for statistics germination length;S3, using the training image after marking, detection label and segmentation label training obtain the artificial intelligence model for detection and segmentation;S4, to be measured image is input in the artificial intelligence model for detection and segmentation, obtain pollen grain count result and germination pollen tube true length;S5, according to pollen grain count result and germination pollen tube true length, determine and statistics camellia pollen germination rate and germination length.Solve the image processing tedious, workload after collecting camellia pollen tube germination and other technical problems.

The application provides a method for reducing the fruit setting rate of side flowers of fruit trees, which utilizes the time difference between the opening of central flowers and side flowers, and sprays a viscous polysaccharide solution at the side flowers after the central flowers have opened for 3-4 hours. The viscous polysaccharide solution can block the stigma of the side flowers by using its viscosity, block the growth of pollen tubes, and finally make the side flowers unable to set fruit due to the inability to pollinate and fertilize, thereby reducing the fruit setting rate of the side flowers and achieving the purpose of controlling the load. The method is simple, labor-saving, safe and efficient, has a low fruit setting rate of the side flowers, and is suitable for fruit products that need to be thinned in large and small orchards.

This invention relates to the field of image processing technology, specifically disclosing an automatic identification and statistical analysis method and system for pollen grains and pollen tubes. For pollen germination images, this method constructs an improved Mask R-CNN recognition model by combining channel attention and spatial attention mechanisms. The model's output mask image undergoes mask localization and Canny edge refinement processing, followed by skeleton extraction from the edge-refined mask image. This enables automatic calculation and batch output of key indicators such as the number of pollen grains, the number of pollen tubes, pollen tube length, and pollen germination rate in the image. It is widely applicable to plant reproductive biology research, pollen viability evaluation, crop breeding, and other fields.