60 results about "Pollen tube" patented technology

The invention discloses application of arabinogalactan protein family genes PbrAGP16 in promoting growth of pear pollen tubes, and belongs to the technical field of plant genetic engineering. According to the invention, the gene PbrAGP16 is cloned from pollen of Dangshan pears by using a plant gene cloning technology, and the nucleotide sequence of the gene PbrAGP16 is shown as SEQ ID No.1; the amino acid sequence coded by the gene is SEQ ID No.2 in a sequence table. Researches show that PbrAGP16 can promote growth of pear pollen tubes, the change of PbrAGP16 expression is related to the change of the content of cellulose at the top ends of the pollen tubes, and PbrAGP16 also participates in a regulation mechanism of a self-incompatible reaction. The pollen magnetic transfection technology is adopted to research the function of the gene in the pollen tube, and a wide application prospect is provided for improving the pollination efficiency.

The invention discloses application of a pear transcription factor PbrWHY2 gene in promoting growth of pear pollen tubes, and belongs to the technical field of plant genetic engineering. According to the invention, the gene PbrWHY2 is cloned from pollen of Dangshan pears by using a plant gene cloning technology, and the nucleotide sequence of the gene PbrWHY2 is shown as SEQ ID No.1; the amino acid sequence coded by the gene is SEQ ID No.2 in a sequence table. The PbrWHY2 can promote the growth of a pear pollen tube, the change of the expression of the PbrWHY2 is related to the change of ROS level and cellulose content at the top end of the pollen tube, and meanwhile, the PbrWHY2 also participates in a regulation mechanism of a self-incompatible reaction to regulate a downstream gene PbrAGP16 / PbrRALF6 / PbrGIS1 in a targeted manner. The pollen magnetic transfection technology is adopted to research the function of the gene in the pollen tube, and a wide application prospect is provided for improving the pollination efficiency.

The invention discloses a method for determining effective pollination periods in different flowering periods of fructus amomi. The method specifically comprises the following steps: (1) monitoring temperature and humidity in real time at different time in the flowering period; (2) observing stigma receptivity through fluorescence microscopy; (3) observing the growth characteristics of the pollen tube through fluorescence microscopy; (4) observing the service life of ovules through fluorescence microscopy; (5) counting changes of fruiting rates of different flower ages; and (6) determining effective pollination periods in different flowering periods. According to the method, the concept of the effective pollination period of Williams is borrowed, the three-element vitality change of the effective pollination period of fructus amomi and the change condition of the fruiting rate of different flower ages in different flowering periods from April to June under different temperature and humidity conditions are researched, and the influence of environment temperature and humidity in different periods on the three-element vitality and the fruiting rate of the effective pollination period is determined; the optimal atmosphere temperature and humidity conditions and effective pollination period time in the fructus amomi flowering and pollination period are expected to be found, and a theoretical basis is provided for improving the fructus amomi fruiting rate and yield through fructus amomi flowering phase temperature and humidity management in production.

The invention discloses a management method for improving cold resistance and drought resistance of macadamia nuts and increasing flowering quantity, which combines a four-stage dynamic management mode of soil improvement, cold resistance induction, drought resistance and tip control and flowering phase quality improvement, applies an organic fertilizer, a water-retaining agent and a composite slow-release fertilizer, induces root development, slowly releases the fertility of a chemical fertilizer, prolongs the action time of the chemical fertilizer, and improves the yield of the macadamia nuts. Brassinolide and potassium fulvate are compounded to activate cold-resistant gene expression of macadamia nut plants and improve cell membrane stability, so that cold resistance of the macadamia nut plants is improved, melatonin and methyl jasmonate perform double shoot control, an ABA signal channel of the plants is triggered, drought resistance of the plants is improved, flower bud differentiation is promoted, and growth of the macadamia nut plants is promoted. The synergistic effect of alginate oligosaccharide and gamma-aminobutyric acid enhances the elongation ability of a pollen tube, chemical agent stimulation is replaced by biological agent stimulation, the utilization rate of chemical agents is reduced, then the negative influence of the chemical agents on soil is reduced, and finally the fruit setting rate and the sustainable utilization of the soil are improved.

The invention discloses application of a brassica juncea gene BjuABCG42-A03 in regulating and controlling pollen germination and seed setting amount of brassica juncea, and relates to the technical field of plant genetic engineering, the amino acid sequence of the protein BjuABCG42-A03 is as shown in SEQ ID No.1, and meanwhile, the invention provides a method for cultivating a transgenic plant capable of improving the pollen germination rate or seed setting amount. The method comprises the following steps: constructing a recombinant plant expression vector containing a gene for coding protein BjuABCG42-A03; the constructed recombinant plant expression vector is transformed into agrobacterium and then transformed into a plant or a plant cell, and the gene of the coded protein BjuABCG42-A03 is over-expressed in the plant, so that the pollen germination rate or the seed setting amount of the plant is increased. Based on gene information of arabidopsis ABCG42 (At4g15233), a BjuABCG42-A03 gene is homologous cloned in a mustard genome, in-vivo and in-vitro germination experiments show that the BjuABCG42-A03 gene affects the elongation length and seed bearing quantity of a pollen tube, and a research direction is provided for solving the problem of low pollen fertility or low seed setting quantity of mustard subsequently.

The application discloses a kind of blending fertilizer raw material potash fertilizer and its preparation method, including the following weight parts of raw materials: 980 parts of potassium sulfate powder, 3 parts of zinc sulfate monohydrate, 10 parts of magnesium sulfate heptahydrate, 2 parts of sodium octaborate tetrahydrate, 1 part of DA-7, 4 parts of alginic polysaccharide.Seven water magnesium sulfate replenishes magnesium element, promotes the synthesis of carbohydrates, fats and proteins, is essential to maintain the green and healthy growth of crop leaves, and is conducive to improving the overall growth vigor and yield of crops.Four water sodium octaborate provides boron element, can promote pollen germination and pollen tube elongation, improve the flowering rate of crops, reduce the phenomenon of flower and fruit drop, DA-7 can promote plant cell division and elongation, improve the photosynthetic efficiency of plants, enhance the stress resistance and nutrient absorption capacity of plants, make crops grow more robustly, and improve fertilizer utilization.

The invention provides a method for improving hybrid breeding efficiency of small-fruit camellia oleifera, and belongs to the technical field of crop breeding. The method comprises the following steps: spraying a 1%-2.5% compound sodium nitrophenolate solution from the early stage of flower bud differentiation of a hybrid male parent to the stage that flower buds begin to expand; spraying a mixed solution of 10-20mg / L gibberellin and 0.1%-0.3% borax at the early bloom stage of the hybrid male parent; male parent pollen is collected for pollination in the full-bloom middle stage. The compound sodium nitrophenolate lays a good foundation for pollen germination and fruit development in the flower bud differentiation and flower development stages, gibberellin promotes pollen germination and pollen tube growth, borax plays an important role in the pollen tube growth and fertilization process and the later fruit development stage, and the compound sodium nitrophenolate, the gibberellin and the borax cooperate with one another to achieve the effect of promoting pollen germination and fruit development. The pollination and fruit development processes of the small-fruit camellia oleifera are jointly promoted, and the hybridization breeding efficiency is improved.

The application belongs to the technical field of plant genetic engineering, and discloses a functional gene PbCOB.A.1 and application thereof. The in-vitro expressed recombinant protein PbCOB.A.1 can promote pollen tube growth of Rosaceae fruit trees, improve pollination efficiency, and expand the regulation mechanism of non-S factors participating in self-incompatibility reaction in pears. The mechanism of pear pollen tube elongation is researched by using magnetic transfection technology, so that the labor cost can be greatly reduced, and a theoretical basis is provided for pear breeding work.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses a nitrate ion transport channel gene PbrSLAH3b and application of the nitrate ion transport channel gene PbrSLAH3b in promoting growth of pear pollen tubules. The method comprises the following steps: extracting total RNA (Ribonucleic Acid) of Dangshan pears from pollen of Dangshan pears; carrying out reverse transcription on the RNA to obtain cDNA (complementary deoxyribonucleic acid); and by taking the cDNA as a template, carrying out amplification to obtain the pear pollen tube nitrate transport channel gene PbrSLAH3b, the PbrSLAH3b gene provided by the invention belongs to a slow anion channel SLAC / SLAH family member, the PbrSLAH3b is remarkably up-regulated in expression in the early growth stage of a pollen tube, and the PbrSLAH3b is positioned in a plasma membrane. The invention verifies that PbrSLAH3b can form a functional anion channel with NO3 <-> selectivity under the condition of not depending on proton gradient or phosphorylation state, and the PbrSLAH3b has relatively strong selectivity on NO3 <->. Therefore, the PbrSLAH3b gene can mediate the absorption and transport of the pollen tube to NO3 <-> and maintain the polarity growth directivity of the pollen tube, and has important application value for revealing the ion regulation mechanism of the growth of the fruit tree pollen tube and improving the reproductive development of the fruit tree.

The application discloses application of a CCHC type zinc finger protein PbrZFP719 gene in promoting pear pollen tube growth and belongs to the technical field of plant genetic engineering. The application clones the gene PbrZFP719 from 'yellow flower pear' pollen by using a plant gene cloning technique, the nucleotide sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence coded by the gene is shown in the sequence table SEQ ID No. 2. Research shows that the PbrZFP719 gene can promote pear pollen tube growth, the change of PbrZFP719 gene expression is related to the change of ROS level and cellulose content at the top of the pollen tube, and meanwhile, the PbrZFP719 gene also participates in the regulation mechanism of self-incompatibility reaction. The pollen magnetic transfection technology is used to study the function of the gene in the pollen tube, and provides a wide application prospect for improving pollination efficiency.

This invention belongs to the field of vegetable cultivation technology, specifically relating to a treatment method for enhancing the low-temperature resistance of tomato anthers and its application. The treatment method for enhancing the low-temperature resistance of tomato anthers includes the following steps: spraying with methyl jasmonate during the pre-opening stage of tomato flowers in a low-temperature environment; the low-temperature environment refers to an ambient temperature below 15℃. This treatment method can effectively alleviate the decline in pollen viability, reduced pollen tube germination rate, and adverse effects on fruit phenotype caused by low temperatures, thereby significantly mitigating the decrease in tomato fruit set rate caused by low-temperature injury.

The application discloses a fruit tree load intelligent decision-making method based on pollen tube behavior and carbon accumulation balance, and belongs to the field of fruit tree growth management. The method collects physiological data such as fruit tree pollen tube growth and leaf photosynthesis in real time through multiple sensors, combines environmental factors and carbon balance parameters, constructs a multi-source data fusion model, and realizes fine perception and dynamic optimization and regulation and control of fruit load under different growth states of fruit trees. The system introduces an abnormal data detection and self-adaptive evolution mechanism to improve the adaptability and robustness of the model to complex field environments and various varieties. The application can effectively improve the yield and quality of fruit trees, optimize the utilization efficiency of carbon resources, and promote the intelligent and fine management of orchards.

The invention relates to the technical field of forest tree genetic breeding and plant reproduction regulation and control, and particularly discloses a black poplar and white poplar distant hybridization obstacle breakthrough method based on sodium benzoate treatment. According to the method, female flowers of black poplar are taken as a treatment object, a sodium benzoate aqueous solution with the final concentration of 5-15 mM (preferably 10 mM) is used for carrying out dipping treatment on the female flowers, and then poplar pollen pollination is carried out. After pollination, fixing, softening and aniline blue dyeing are carried out, and pollen germination and pollen tube growth conditions are observed. Experimental results show that the method can significantly promote the growth of the poplar pollen on the black poplar stigma, improve the ability of a pollen tube to penetrate through the stigma, and realize fertilization and hybrid embryo formation, thereby effectively breaking through the distant hybridization obstacle between different branches of the poplar. The method disclosed by the invention is simple and convenient to operate, low in cost and good in repeatability, provides an efficient and feasible technical means for distant hybridization breeding of poplars, and has a good popularization and application prospect.

A method for rapidly determining the viability of watermelon pollen, comprising the following steps: preparing a stock solution: adding 0.9 - 1.2 g of anhydrous CaCl<subgt;2< / subgt>, 0.9 - 1.0 g of KNO<subgt;3< / subgt>, 0.3 - 0.5 g of MgSO<subgt;4< / subgt>·7H<subgt;2< / subgt>O, 0.1 - 0.3 g of KCl, and 0.1 - 0.12 g of H<subgt;3BO<subgt;3 to 600 - 800 mL of water, completely dissolving and then making up the volume to 1 L to obtain the stock solution, storing it at room temperature; preparing the viability detection culture medium: adding 1.0 - 1.5 g / 10 g of sucrose to the above-prepared stock solution, and obtaining the viability detection culture medium after the sucrose is completely dissolved; viability detection: laying a filter paper moistened with water in a petri dish, placing a glass slide on the filter paper, dropping 1 - 2 drops of the viability detection culture medium on the glass slide, dipping the fresh watermelon flowers to be detected in the culture medium several times, at this time the number of pollen grains can be observed, covering the petri dish lid, and culturing at room temperature for 20 - 30 min; observing the pollen tube germination situation and calculating the pollen viability percentage. Using the culture medium of the present invention to culture pollen, the result can be observed in only 20 - 30 min, and it only needs to be placed at room temperature during the culture without special requirements.

The present invention discloses an application of a rice THION22 small peptide protein in breaking the reproductive isolation between indica and japonica subspecies of rice, belonging to the field of biotechnology. The present invention breaks the pre-zygotic reproductive isolation of the indica and japonica subspecies of rice by applying the rice THION22 small peptide protein in vitro during the indica-japonica hybridization process. The application includes the rice THION22 small peptide protein promoting the growth of japonica rice pollen tubes in the pistil of the indica rice variety, and the rice THION22 small peptide promoting the acquisition of indica-japonica hybrid F1 hybrid seeds. The present invention effectively promotes the hybridization of japonica rice pollen with some indica rice varieties, promotes the growth of japonica rice pollen tubes, produces more F1 hybrid seeds, improves the efficiency of indica-japonica hybridization breeding, and has important application value for rice hybridization breeding and heterosis practice.

The invention discloses an oat glume-cutting hybridization method capable of increasing the maturing rate and application thereof.According to the oat glume-cutting hybridization method, by conducting castration and glume-cutting treatment on a first main grain of an outermost spikelet of each round layer in a female parent, it can be guaranteed that ovules obtained after artificial hybridization pollination have sufficient nutrition for grain filling according to the oat fruiting nutrition distribution rule; female parents are bagged after castration and glume cutting, the bagging time is controlled to be 12-24 h, the water content of the fluff surface of the stigma can be reduced (it is prevented that pollen is separated by a water film when falling on the stigma), the contact area of the pollen and the stigma is increased, and the germination rate of pollen tubes is increased; pollination is carried out when the male parent anther naturally cracks, that is, pollination is carried out in the period with the maximum pollen viability, and the cross pollination probability can be increased. According to the method, the hybridization maturing rate can be remarkably increased, and the hybridization maturing rate can reach 70% or above when the white swallow No. 2 is used as the female parent and the white swallow No. 7 is used as the male parent for hybridization.

The invention provides application of citrus S-adenosylmethionine synthetase in improving self-incompatibility, and belongs to the technical field of biological genetic engineering, and the gene sequence of the citrus S-adenosylmethionine synthetase is shown as SEQ ID: 1. Experiments prove that citrus S-adenosylmethionine synthetase directly interacts with S-RNase to regulate and control a methionine metabolic pathway so as to influence the growth of a pollen tube; the invention discloses a dual regulation and control effect of the citrus S-adenosylmethionine synthetase in a self-incompatible reaction, and provides a new target for improving the self-compatibility of crops. According to the method, the citrus S-adenosylmethionine synthetase is added into pollen culture or methionine is sprayed onto styles, so that the growth of pollen tubes is improved, the self-incompatibility of the citrus is further improved, a new strategy is provided for improving the self-incompatibility of crops, and the problems of low fruit setting rate, high artificial pollination cost and the like caused by the self-incompatibility are hopefully solved.

The invention relates to the technical field of durian planting, and particularly provides a regulation and control method for promoting and protecting flowers of durian and a medicament. Through comprehensive measures such as environment regulation and control, nutrition management, medicament treatment and flowering period management, flowering and flower protection of the durian are effectively promoted, and the fruit setting rate is increased. Specifically, by controlling the drought period, adjusting the environmental factors such as illumination and humidity, and combining the application of the fish protein fermentation broth and the flower-promoting and flower-protecting agent for the durian, suitable conditions are provided for the growth and flowering of the durian. Meanwhile, monopotassium phosphate, gibberellic acid, calcium amino acid chelate and a boric acid solution are sprayed in the flowering period, and flower bud differentiation and development and pollen tube growth and fertilization are further promoted. The method and the agent are low in cost, easy and convenient to operate, environmentally friendly, safe and suitable for large-scale application and popularization, and the yield and economic benefits of the durian can be remarkably improved.

The application discloses application of a rapid alkalization factor family gene PbrRALF6 in promoting pear pollen tube growth and belongs to the technical field of plant genetic engineering. The application clones the gene PbrRALF6 from 'Dangshan quxi pear' pollen by using plant gene cloning technology, the nucleotide sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence coded by the gene is shown in the sequence table SEQ ID No. 2. Research shows that PbrRALF6 can promote pear pollen tube growth, the change of PbrRALF6 expression is related to the change of the ROS level at the top of the pollen tube, and meanwhile, PbrRALF6 also participates in the regulation mechanism of self-incompatibility reaction. The pollen magnetic transfection technology is used to study the function of the gene in the pollen tube, thereby providing wide application prospects for improving pollination efficiency.

This invention provides the application of citrus S-adenosylmethionine synthase in improving self-incompatibility, belonging to the field of biogenetic engineering technology. The gene sequence of citrus S-adenosylmethionine synthase is shown in SEQ ID:1. This invention experimentally demonstrates that citrus S-adenosylmethionine synthase affects pollen tube growth by directly interacting with S-RNase and regulating the methionine metabolic pathway. This invention reveals the dual regulatory role of citrus S-adenosylmethionine synthase in self-incompatibility responses, providing a new target for improving crop self-compatibility. This invention improves pollen tube growth by adding citrus S-adenosylmethionine synthase to pollen culture or spraying methionine onto the style, thereby improving citrus self-incompatibility. This provides a new strategy for improving crop self-compatibility and is expected to solve problems such as low fruit set rate and high artificial pollination costs caused by self-incompatibility.

The invention relates to the technical field of plant genetic engineering, and particularly discloses application of an alfalfa MsWRKY49 gene to regulation and control of elongation of a pollen tube under low boron stress. Researches find that the MsWRKY49 gene responds to low-boron stress through an active oxygen (ROS) signal channel, and the expression quantity of the MsWRKY49 gene is remarkably increased under a low-boron condition. The construction of an MsWRKY49 overexpression strain and a gene editing mutant proves that the gene has a negative regulation effect on the elongation of a pollen tube: the length of the pollen tube of an overexpression plant is shortened under a low-boron condition, the rupture rate is increased, and the mutant presents an opposite phenotype. According to the invention, the function of the MsWRKY49-ROS regulation module in boron signal transduction is disclosed for the first time, and new gene resources and molecular breeding strategies are provided for genetic improvement of crops in boron-deficient regions.

The invention discloses a method for determining pollination affinity among camellia oleifera varieties based on pollen tube in-situ germination, and belongs to the technical field of agricultural planting. The method comprises the following steps: collecting pistil samples which are pollinated for 48-72 hours, fixing and slicing, and quantifying the pollination affinity by adopting fluorescence microscopic observation in combination with a five-level weighted scoring system (1 score of stigma, 2 scores of upper part of stylus, 3 scores of lower part of stylus, 4 scores of ovary cavity and 5 scores of ovule). Compared with a traditional fruit setting rate method, the method has the advantages that the detection period is shortened from 1-3 months to 3 days, the affinity of different pollination variety combinations can be distinguished more accurately, and the method is particularly suitable for variety matching selection in camellia oleifera cross breeding.

The invention discloses application of an HIRA gene in regulation and control of plant male fertility, and belongs to the technical field of plant gene functions. According to the invention, CRISPR-Cas9 gene editing vector construction is carried out on a rice gene OsHIRA1, a rice variety Nipponbare is transfected by the vector, an obtained gene editing plant is identified and observed, a homozygous mutant cannot be obtained, in-vitro and in-vivo pollen tube germination experiments are carried out on heterozygous mutant pollen, and half of pollen tubes germinate abnormally. It can be known that the rice OsHIRA1 participates in the pollen tube germination process. The method can be used for breeding hybrid rice and provides a new reference thought for breeding strategies of other species.

The invention belongs to the technical field of plant transgenosis, and particularly relates to a nano magnetic bead mediated pennisetum alopecuroides pollen tube channel transgenosis method. The method comprises the following steps: compounding magnetic beads and plasmid DNA, compounding a magnetic bead-DNA compound and a pollen culture solution, carrying out magnetic transfection, and pollinating. Compared with a traditional transformation mode, the transformation method mediated by the nano magnetic beads does not need tissue culture, transformation conditions are milder, transformation time is shorter, and the transformation method is an efficient and rapid transformation mode and has great guiding significance on genetic breeding work of pennisetum alopecuroides; through artificial pollination, a large number of seeds can be obtained, and more materials are provided for subsequent screening of transgenic plants; the method provided by the invention is simple to operate, saves resources, and achieves an excellent effect through a synergistic effect among the operation steps.

The present invention relates to the field of biotechnology, and in particular to a method for observing the growth behavior of pollen tubes of macadamia nuts. Specifically, the method comprises the following steps: (1) immersing the pollinated pistil in a fixing solution for fixing; (2) soaking the pistil in an alcohol solution and then washing it; (3) mixing the pistil with a NaOH solution to soften it, washing it, and absorbing the water; (4) soaking the pistil in a phosphate buffer solution; (5) cross-cutting the pistil at the middle position to separate the stigma of the upper half of the pistil from the ovary of the lower half, and then cutting it into two halves longitudinally, and soaking it in a phosphate buffer solution again; (6) staining the pistil with a dye after absorbing the water, and microscopically examining it. The fluorescence microscopy method of the present invention greatly weakens the phenomenon of luminescence of the pistil's own tissue, and it is easy to obtain a high-definition image of the pollen tube growing in the pistil. The method has an ideal presentation effect on the growth behavior of the pollen tube, and can be used for the hybridization affinity evaluation between macadamia nut varieties, and has high practical application value.

The pollen activation solution (1) contains an aqueous solution containing a substance for activating the germination of pollen and the elongation of a pollen tube, and a powdered carbon-constituting substance added to the aqueous solution. The carbon-constituting material is not activated. For example, sucrose, boric acid, magnesium sulfate heptahydrate, calcium chloride, potassium chloride, gelatin or the like can be used as a substance for activating the germination of pollen and the elongation of pollen tubes. For example, a bamboo leaf charcoal powder obtained by pulverizing bamboo leaf charcoal can be used as the powdered carbon constituent material.

The invention discloses a fluorescence microscopic detection method for accurately detecting villous amomum fruit stigma pollination. The method specifically comprises the following steps: (1) bagging and pollination: bagging to-be-detected villous amomum fruit flowers before flowering, and artificially pollinating to-be-detected stigmas; (2) sampling treatment: 24-48 hours after pollination, taking off a bag, collecting fructus amomi flowers, stripping a complete pistil, and sequentially carrying out FAA fixation, NaClO solution transparentizing, NaOH solution softening and aniline blue solution dyeing treatment; (3) loading and observing, namely cutting off the treated pistil from the joint of the stylus and the top end of the ovary, respectively preparing a'stigma and stylus' piece and a'ovary 'piece, and observing the germination and growth condition of pollen on the stigma under a fluorescence microscope; (4) determining stigma pollination: determining the stigma pollination according to three indexes, namely the germination rate of pollen on the stigma, the growth position of the pollen tube on the stylus and whether the pollen tube enters the ovary. The method disclosed by the invention can be used for accurately detecting the stigma receptivity of the fructus amomi, and lays a foundation for research on insemination and reproduction of fructus amomi flowers and increase of the fruiting rate in the future.

A pollen activation solution (1) includes: an aqueous solution containing a substance that activates pollen germination and pollen tube elongation; and a powdered carbon constituent added to the aqueous solution. The carbon constituent has not been subjected to an activation treatment. Examples of the substance that activates pollen germination and pollen tube elongation, which can be used herein, include sucrose, boric acid, magnesium sulfate heptahydrate, calcium chloride, potassium chloride, and gelatin. An example of the powdered carbon constituent that can be used herein is bamboo leaf charcoal powder made by pulverizing bamboo leaf charcoal.

The invention belongs to the technical field of agriculture and biology, discloses application of arabidopsis thaliana GALT4 in regulation and control of pollen development, and verifies that the arabidopsis thaliana GALT4 gene is a plant male fertility key regulation and control gene with high pollen specificity expression, and the CDS sequence is shown as SEQ ID NO.1 for the first time. Polarity elongation of the pollen tube is maintained by regulating and controlling spatial and temporal distribution of methyl esterified pectin in the pollen tube. Knockout or silencing of the homologous gene in a plant can lead to reduction of pollen viability, abnormal development of pollen cell nucleus, malformation of pollen morphology, slowing of pollen germination rate and hindering of pollen tube polarity elongation. The invention provides a brand new molecular target for plant pollen morphological development and pollen tube growth regulation and control, can be used for fertility regulation and control and hybrid seed production optimization of cruciferous crops, and has important theoretical research value and industrial application prospect.

The invention discloses a method for improving the natural pollination success rate of Lingyun Niuxin plums, and belongs to the technical field of fruit tree cultivation. The method comprises the following steps: spraying a pollen activity enhancer compounded by compound sodium nitrophenolate, borax, monopotassium phosphate and zinc sulfate at the initial flowering stage of pollinizers so as to remarkably improve the pollen activity and timeliness; spraying superfine water mist by adopting a high-pressure mist spraying machine in the early morning of the full-bloom stage so as to optimize the pollination microenvironment, prolong the pollination ability of stigmas and promote the growth of pollen tubes; in a key period after blossom fall, compound fertilizer is immediately dressed, and a foliar nutrient solution containing urea and calcium chloride is sprayed to guarantee young fruit development and prevent physiological fruit drop. Through the synergistic effect of the systematic technical scheme, the defect that traditional natural pollination depends on the day to eat is thoroughly overcome, on the premise that artificial pollination is not conducted, the fruit setting rate can be remarkably increased to 68.5% or above, meanwhile, the fruit cracking rate is effectively reduced, unification of high yield and high quality is achieved, the core bottleneck problem of Lingyun Niuxin plum industry development is solved, and the market prospect is wide. The economic benefit is obvious.