51 results about "Microtubule" patented technology

The invention discloses an application of CLASP2 protein in preparation of a medicine for promoting spinal cord injury repair. According to the invention, a bioinformatics and phosphorylated proteomics technology combined method is utilized to screen out that the functional protein of curcumin for promoting spinal cord injury repair is CLASP2 protein. The molecular biological technique verifies that curcumin regulates and controls microtubule stability by increasing phosphorylation of CLASP2 protein to promote functional repair after spinal cord transection injury. Therefore, the curcumin can regulate and control microtubules by promoting the phosphorylation of the CLASP2 to dynamically promote the motor function repair after the spinal cord injury of the mouse. The method has an important application value.

The application provides a construction of a fluorescent protein labeled microtubulin and a microtubule binding protein universal bivalent carrier, and the process is as follows: a first stage is to construct a GFP-alpha tubulin carrier, and a second stage is to construct a GFP-alpha tubulin-mCherry universal bivalent co-expression carrier; in the second stage, 35S, mCherry and NOS sequences are inserted into a KpnI enzyme cutting site of a multiple cloning site of the GFP-alpha tubulin carrier constructed in the first stage, and a single nucleic acid enzyme cutting site is inserted into the 5' end and the 3' end of the mCherry sequence, respectively, and the single nucleic acid enzyme cutting site is XbaI, KpnI / Acc65I and AscI sequences, respectively, so as to obtain the universal bivalent carrier. The universal bivalent carrier provided by the application can express two target genes simultaneously, has the fluorescent signals of GFP and mCherry, can quickly and accurately identify a transgenic plant, is convenient for positive seedling screening, can be used for a tobacco transient expression experiment, can shorten an experimental period, and saves time and effort.

The present invention provides a retractable drug perfusion stent system, which belongs to the field of medical device technology, and includes: an inner tube and an outer tube slidably sleeved on the inner tube, the proximal ends of the inner tube and the outer tube are connected with a pushing device; the distal end of the inner tube has a tip; a perfusion microtube, which is spiral-shaped and has micropores; a perfusion tube; and a drug delivery device. The retractable drug perfusion stent system provided by the present invention is provided with micropores on the perfusion microtube. After the perfusion microtube reaches the lesion position, the perfusion microtube is switched from a retracted state to an expanded state. At this time, the perfusion microtube is freed from the restriction of the outer tube, and expands itself to fit with the blood vessel wall and expand the target lesion. The drug delivery device inputs the drug into the perfusion microtube through the perfusion tube, and delivers the drug to the target lesion through the micropores of the perfusion microtube. This drug perfusion stent will not remain in the body. At the same time, this method will not block blood flow, and can also continuously deliver drugs and control the drug delivery time. The entire operation process is simple and easy to operate.

The present invention discloses a method for cell cycle labeling and identification based on organic mass spectrometry detection technology, belonging to the technical field of cell biology. Two types of small molecule tags, namely DNA-targeting molecules and microtubule-targeting molecules, are used to label cells, and the optimized labeling conditions can label living cells. Such tags have good compatibility with organic mass spectrometry detection technology, are applicable to various ionization modes of organic mass spectrometry analysis, have high dissociation efficiency during the ionization process, and good mass spectrometry response. The tag signals and intracellular endogenous lipid signals can simultaneously serve as the basis for cell cycle differentiation and identification, and have good differentiation and identification effects on the G0 / G1, S, G2, and M cell cycle stages. While identifying the single-cell cycle, this method can collect single-cell metabolite information, and further study cell metabolism-related activities at specific cell cycle stages, and has broad application prospects in the fields of stem cell analysis, tumor diagnosis, and systems biology research.

The present disclosure relates to polynucleotides and methods of inhibiting the activities of development-associated pathways, including the Wnt, Hedgehog, and Notch pathways, and the invasive capacity of malignant cells to treat primary or secondary solid tumors.

This invention relates to compounds which are microtubule associated serine / threonine-like kinase (MASTL) inhibitors and the use of the compounds in the treatment of diseases and medical conditions mediated by MASTL, for example in the treatment of cancer and other target related diseases.

The application discloses a kind of porous carbon load PEDOT microtubule composite water collection materials and preparation method and application thereof, belong to water collection material preparation field.The material includes porous carbon and its surface load PEDOT microtubule, and the PEDOT microtubule is poly 3,4-ethylenedioxythiophene with microtubule structure.The preparation of the material is that oxidizing compound M and carbon source are mixed, carbonization is carried out to obtain porous carbon precursor;Again, the porous carbon precursor is polymerized with 3,4-ethylenedioxythiophene monomer and acid in closed environment to obtain.The composite water collection material of the application shows efficient, fast, reversible atmospheric water capture capacity in wide humidity range, and excellent cycle stability, with significant engineering practical value and industrialization prospect.

The invention belongs to the technical field of medicines, and particularly relates to application of arginine in repairing skeletal muscle injury caused by monensin poisoning. It is found for the first time that arginine can remarkably recover the normal function of skeletal muscle mitochondria and the normal polymerization capacity of microtubules by up-regulating the expression level of mitochondrial oxidative phosphorylation (OXPHOS) protein complex and regulating microtubule polymerization kinetics and reversing monensin-induced skeletal muscle mitochondrial dysfunction and microtubule network disintegration; the method provides a theoretical basis for clinical transformation, and has good practical application value.

In one aspect, methods of diagnosing a subject as having Alzheimer's disease and prognosing a subject as being at risk of progressing to Alzheimer's disease are provided. In some embodiments, the method comprises determining one or more of the level of expression of rhotekin 2 (RTKN2), the level of expression of microtubule-associated Ser / Thr kinase 4 (MAST4), the level of binding of forkhead box O1 (FOXO1) to the RTKN2 promoter, and the level of binding of amyloid precursor protein (APP) to the MAST4 promoter in a sample from the subject.

This invention relates to compounds which are microtubule associated serine / threonine-like kinase (MASTL) inhibitors and the use of the compounds in the treatment of diseases and medical conditions mediated by MASTL, for example in the treatment of cancer and other target related diseases.

The invention provides construction and application of an intermolecular structural domain tension probe, the probe comprises a first structure, a second structure and a fluorescent protein pair eCFP and eYFP, the first structure and the second structure are respectively connected to two ends of the fluorescent protein pair, and the eCFP and the eYFP are connected through a short peptide; the first structure is a structural domain or protein capable of being combined with a cytoskeleton connecting protein, and the second structure is a structural domain or protein capable of being combined with a cytoskeleton; the cytoskeleton connecting protein is selected from talin, SYNE4, SYNE3, SYNE2, Desmoplakin and the like; the cytoskeleton is a microfilament, a microtubule or an intermediate filament. The intermolecular structural domain tension probe can be used for detecting intracellular mechanical signals, the limitation of a traditional tension probe is overcome, the adverse effect of function enhancement caused by gene cloning is avoided, a new opinion is provided for a mechanical transduction molecular mechanism, and the application has the advantages that the application range is wide, and the application prospect is wide. And a more accurate experimental tool is provided for the research of cell mechanics biology, and the effect of a mechanical signal in cell behavior regulation and control is disclosed.

The invention discloses a composite catchment material of a porous carbon-loaded PEDOT (poly (3, 4-ethylenedioxythiophene)) microtube as well as a preparation method and application of the composite catchment material, and belongs to the field of catchment material preparation. The material comprises porous carbon and a PEDOT (poly (3, 4-ethylenedioxythiophene)) microtube loaded on the surface of the porous carbon, and the PEDOT microtube is poly (3, 4-ethylenedioxythiophene) with a microtube structure. The preparation method of the material comprises the following steps: mixing an oxidizing compound M and a carbon source, and carbonizing to obtain a porous carbon precursor; and carrying out in-situ polymerization reaction on the porous carbon precursor, a 3, 4-ethylenedioxythiophene monomer and acid in a closed environment to obtain the porous carbon material. The composite water collecting material shows efficient, rapid and reversible atmospheric water capturing capability in a wide humidity range, is excellent in cycling stability, and has remarkable engineering practical value and industrialization prospect.

The invention relates to generation, optimization and characterisation of VHH targeted against Tau MTBD (microtubule-binding domain) with high affinity, obtained by screening from a naïve synthetic library. The inventors optimized version of a lead VHH which is able to inhibit Tau aggregation in vitro and in HEK 293 aggregation-reporting cellular model, providing a new tool in Tau immunotherapies. Accordingly the invention relates to new VHH antibody that specifically binds with high affinity Tau species, especially the epitope region involved in Tau aggregation. Moreover, the inventors found that immunization with the optimized version of this lead VHH prevented the formation of neurofibrillary tangles induced by injection of extracellular h-AD in mouse model. Thus, these specific antibodies can be used for the therapy of tauopathy disorders such as Progressive supranuclear palsy (PSP).

The invention provides a compound as shown in a formula (I), a pharmaceutical composition as well as a preparation method and application of the compound. The compound has a good KIF18A inhibition effect, can form a binding complex independently or with a microtubule to regulate KIF18A protein, and is used for treating KIF18A-mediated diseases and / or diseases, such as tumor diseases, and preparing drugs for the diseases or diseases. # imgabs0 #

The present disclosure relates to modulation of microtubule dynamics as therapeutic targets for neural restoration. There is provided a method of treating nerve injury in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of a therapeutic agent wherein the therapeutic agent is selected from the group consisting of metaxalone and a formamidin-2 inhibitor, such as an antibody, an antibody fragment, an inhibitory nucleic acid molecule or a small molecule, wherein the inhibitory nucleic acid molecule substantially silences Fmn2.

The invention belongs to the field of gene engineering, and particularly relates to a recombinant bluetongue virus with green fluorescent protein carried on an NS1 microtube as well as a construction method and application of the recombinant bluetongue virus. According to the invention, an enhanced green fluorescent protein (eGFP) gene is inserted between the 534 amino acid and the 535 amino acid of a wild type bluetongue virus non-structural protein NS1, and an NS1 recombinant bluetongue virus BTV-NS1-534 eGFP is constructed; the recombinant bluetongue virus BTV1-NS1-534eGFP can form a virus-like microtubule similar to a wild type bluetongue virus (BTV1-WT), the growth characteristics of the virus-like microtubule are not obviously different from those of the BTV1-WT, and the recombinant bluetongue virus BTV1-NS1-534eGFP can correctly express enhanced green fluorescent protein; the method can be used for tracing research of NS1 protein after BTV infects cells, and can also be used for virus neutralization experiment of BTV, visual quantitative detection of virus content and the like.

The present invention relates to compounds that are inhibitors of microtubule-associated serine / threonine-like kinase (MASTL), and to the use of the compounds in the treatment of diseases and medical conditions mediated by MASTL, for example in the treatment of cancer and other target-related diseases.

The utility model provides a quick -plug formula microtubule reducing joint of sealing structure area belongs to precision instrument technical field. Including the connector, still including the reinforcement subassembly, reinforcement subassembly includes setting up the support pipe of connector one side, the one side of support pipe all is seted up with the sliding slot, the inside of sliding slot is provided with the rolling ball and slides, the one side of support pipe is provided with the connecting soft pad, reinforcement subassembly at the time of use, support pipe plays the basic support effect, sliding slot provides the sliding space for the rolling ball, and the rolling ball can be in the flexible movement in the groove, be used for adjusting position, and the connecting soft pad can enhance the sealing property in the connection, reduce the attrition between parts, thereby make the whole reinforcement subassembly realize the effect of firm connection and good sealing in use, the connecting pipe's fixed groove and the connecting soft pad of support pipe joint, enhance the connection sealing property, the connecting pipe slot place is equipped with the rotating shaft, and the shaft interlocking block can rotate around the shaft, and the spring piece of slot place connects the interlocking block, provides the elasticity.

The present application belongs to the technical field of plant root monitoring, and particularly relates to a method for protecting micro-root tubes from being damaged by rodents, which comprises the following steps: identifying specific positions of the micro-root tubes through micro-root tube data acquisition, image classification training, contour feature extraction, alarm identification and video pre-recording, and taking snapshots; using a key algorithm of frame separation to analyze, so as to realize protection of the micro-root tubes and automatic identification and automatic alarm of the micro-root tubes damaged or lost by rodents; after the micro-root tubes are determined and identified, an aluminum alloy pop-top can with a removed top end is sleeved on an exposed end; since the material of the pop-top can is plastic, after the root system is determined, the pop-top can can be pinched to make the pop-top can more closely attached to the micro-root tube, so as to play a role in protecting the micro-root tube.

The invention provides a myocardial cell fusion automatic arrangement method based on a micro-channel, which comprises the following steps: initializing an operation visual field, and placing the micro-channel, myocardial cells and a microtube orifice in the same visual field; based on the current visual field image, myocardial cell detection and micro-channel and micro-tube orifice identification are carried out on the visual field image shot by the camera by using an image processing technology, and myocardial cells are numbered; determining the initial positions of myocardial cells and microtubule orifices and the target position of the microtubule orifice when the myocardial cells are moved and discharged to the microchannel, and planning the moving path of the microtubule; in order to control the position of the myocardial cells in the liquid which is sucked into or discharged out of the microtubule along with the microtubule, a sliding mode surface is constructed according to a myocardial cell movement model, and a sliding mode active-disturbance-rejection controller is designed to control a stepping motor in the process of transferring the myocardial cells by the microtubule; and according to the numbers of the myocardial cells, moving the myocardial cells in the visual field through the microtubes according to a planned path and discharging the myocardial cells into the micro-channels to finish automatic arrangement.

The present invention provides a compound represented by formula (I), a pharmaceutical composition, and methods for preparing and using the same. The compound has good KIF18A inhibitory activity and can regulate KIF18A protein either alone or by forming a binding complex with microtubules, thereby treating KIF18A-mediated disorders and / or diseases, such as neoplastic diseases, and can be used to prepare drugs for such disorders or diseases. [Formula 1] JPEG2025533411000090.jpg30169

The invention belongs to the field of medical biology, and discloses an N, 2, 3-trimethyl-N-fused aryl-2H-indazole-6-amine compound with a structural formula as shown in a formula I in the specification. The N, 2, 3-trimethyl-N-fused aryl-2H-indazole-6-amine compound disclosed by the invention can be used for effectively inhibiting in-vitro proliferation of a plurality of tumor cells; the action mechanism is similar to that of colchicine, and aggregation of microtubules can be inhibited; the potential development and application values are realized.

To provide a new class of compounds useful for modulating KIF18A protein alone or in a bound complex with microtubules, for treating KIF18A-mediated conditions and / or diseases, including cancer, inflammation, or ciliary body lesions.SOLUTION: Chemical compounds having the general formula (I) are provided. (In the formula: X1 is N or -CR6; X2 is N or CR7; X3 is N or CR8; X4 is N or CR9; and R6 to R9 are H, halo, or the like; provided that one, two or three of X1, X2, X3 and X4 are N.)SELECTED DRAWING: None

The present invention provides a medicine for treating a neurodegenerative disease, which employs LEFTY2 (Left-Right Determination Factor 2) generated by co-culturing mesenchymal stem cells of mammals with nerve cells having mutations in the APP (Amyloid precursor protein) gene, or a specific protein. The LEFTY2 has an effect of inhibiting Beta amyloid and a hyperphosphorylated neuronal microtubule-associated protein (Tau protein), without affecting the development of nerve cells and having the ability to promote the growth of the nerve cells; and the two types of proteins have a crucial impact on the neurodegenerative diseases.

The invention discloses application of a substance for inhibiting Sqstm1 gene expression and inhibiting Sqstm1 from transmitting phosphoric acid chemical energy to a microtube in an extracellular vesicle secretion inhibitor, and relates to the technical field of biomedicine. The application method comprises the following steps: screening a substance capable of targeting a transcription product of the Sqstm1, an inhibiting substance targeting protein expression of the Sqstm1, and a substance for inhibiting the Sqstm1 from transmitting phosphoric acid chemical energy to a microtubule. The Sqstm1 is used as a key target of the EVs inhibitor as a core, and the main advantage of the Sqstm1 is that the inhibition of the Sqstm1 does not affect the generation of intracellular vesicles. Therefore, the Sqstm1 can be used as a novel vesicle transportation / secretion inhibition target, and a new strategy and thought are provided for scientific research on vesicle transportation / secretion in the future.

The invention belongs to the technical field of medicines, and particularly relates to application of arginine in repairing skeletal muscle injury caused by monensin poisoning. It is found for the first time that arginine can remarkably recover the normal function of skeletal muscle mitochondria and the normal polymerization capacity of microtubules by up-regulating the expression level of mitochondrial oxidative phosphorylation (OXPHOS) protein complex and regulating microtubule polymerization kinetics and reversing monensin-induced skeletal muscle mitochondrial dysfunction and microtubule network disintegration; the method provides a theoretical basis for clinical transformation, and has good practical application value.

The invention particularly relates to a SnO2-coated In2O3 visible light excitation gas-sensitive material as well as a preparation method and application thereof. In order to solve the technical problems that a SnO2-based NO2 gas sensor is relatively high in working temperature and relatively long in response recovery time in the prior art, the invention provides the SnO2-coated In2O3 visible light excitation gas-sensitive material which is a eutectic material prepared by grinding and heating SnO2 hollow nanospheres and In2O3 hollow microtubes according to an In: Sn atomic ratio of 20-60 at%, and the SnO2-coated In2O3 visible light excitation gas-sensitive material is a SnO2-coated In2O3 visible light excitation gas-sensitive material. After light is added, a large number of photo-generated electrons and photo-generated holes are formed to participate in the reaction, and sensitive and stable detection on nitrogen dioxide under the room temperature condition can be realized.

The invention relates to a method for purifying proteins having a tubulin carboxypeptidase activity from a biological extract, comprising polymerization / depolymerization cycle performed on a biological extract in presence of microtubules. The invention further relates to a peptidic based inhibitor for use in the treatment of a disorder involving altered microtubule detyrosination in an animal, wherein the peptidic based inhibitor comprises a peptidic moiety constituted of 1 to 20 amino acids, said peptidic moiety having an amino acid selected from Y or F at the C-terminal position, and wherein the peptidic based inhibitor inhibits at least partially a tubulin carboxypeptidase activity.

The present invention discloses a method, device and system for calculating water balance of a water diversion project. The method comprises generating a directed graph of a canal system based on the relative positions of canal sections, water diversions and gates in the water diversion project; generating a generalized building model based on the building types of canal sections, water diversions and gates in the water diversion project; generating a canal system topology model based on the directed graph of the canal system and the generalized building model; traversing the canal system topology model according to the water balance theory and the changes in the inflow and outflow of the canal section by using a path search algorithm to generate the paths between each terminal water diversion and the water source; when the flow of one or more water diversions changes at the same time, according to the micro-tube flow bundle theory and the paths related to the water diversions with changed flow, a bottom-up flow superposition method is used to obtain the flow change of the relevant canal section. The present invention can quickly calculate the flow change of each canal section, improve the dispatching accuracy and the work efficiency of personnel.

The present application discloses a method for in situ culture of plant roots and its application. The method comprises: step 1, spreading sterilized Arabidopsis seeds flat on the upper edge of a glass slide culture medium; the thickness of the culture medium is 0.9-1.4 mm; step 2, placing the glass slide with seeds in a culture dish equipped with a culture device and culturing vertically under light; the culture device comprises a circular bottom plate, and a fixing device provided on the circular bottom plate for fixing the glass slide with seeds and a humidifying device for increasing the air humidity in the culture dish. After the vertical culture is completed, the present application can directly prepare slides without moving the Arabidopsis roots and observe the arrangement of microtubules in the root cells under a microscope, thereby avoiding damage and displacement of the Arabidopsis roots when preparing slides using traditional methods; specifically, by selecting a specific device during the vertical culture process, the technical problem that the existing culture dish cannot fix the glass slide, causing the root growth to bend and thus affecting the root observation is overcome.