22 results about "Microtubule" patented technology

The application provides a construction of a fluorescent protein labeled microtubulin and a microtubule binding protein universal bivalent carrier, and the process is as follows: a first stage is to construct a GFP-alpha tubulin carrier, and a second stage is to construct a GFP-alpha tubulin-mCherry universal bivalent co-expression carrier; in the second stage, 35S, mCherry and NOS sequences are inserted into a KpnI enzyme cutting site of a multiple cloning site of the GFP-alpha tubulin carrier constructed in the first stage, and a single nucleic acid enzyme cutting site is inserted into the 5' end and the 3' end of the mCherry sequence, respectively, and the single nucleic acid enzyme cutting site is XbaI, KpnI / Acc65I and AscI sequences, respectively, so as to obtain the universal bivalent carrier. The universal bivalent carrier provided by the application can express two target genes simultaneously, has the fluorescent signals of GFP and mCherry, can quickly and accurately identify a transgenic plant, is convenient for positive seedling screening, can be used for a tobacco transient expression experiment, can shorten an experimental period, and saves time and effort.

The present disclosure relates to polynucleotides and methods of inhibiting the activities of development-associated pathways, including the Wnt, Hedgehog, and Notch pathways, and the invasive capacity of malignant cells to treat primary or secondary solid tumors.

The application discloses a kind of porous carbon load PEDOT microtubule composite water collection materials and preparation method and application thereof, belong to water collection material preparation field.The material includes porous carbon and its surface load PEDOT microtubule, and the PEDOT microtubule is poly 3,4-ethylenedioxythiophene with microtubule structure.The preparation of the material is that oxidizing compound M and carbon source are mixed, carbonization is carried out to obtain porous carbon precursor;Again, the porous carbon precursor is polymerized with 3,4-ethylenedioxythiophene monomer and acid in closed environment to obtain.The composite water collection material of the application shows efficient, fast, reversible atmospheric water capture capacity in wide humidity range, and excellent cycle stability, with significant engineering practical value and industrialization prospect.

The invention belongs to the technical field of medicines, and particularly relates to application of arginine in repairing skeletal muscle injury caused by monensin poisoning. It is found for the first time that arginine can remarkably recover the normal function of skeletal muscle mitochondria and the normal polymerization capacity of microtubules by up-regulating the expression level of mitochondrial oxidative phosphorylation (OXPHOS) protein complex and regulating microtubule polymerization kinetics and reversing monensin-induced skeletal muscle mitochondrial dysfunction and microtubule network disintegration; the method provides a theoretical basis for clinical transformation, and has good practical application value.

In one aspect, methods of diagnosing a subject as having Alzheimer's disease and prognosing a subject as being at risk of progressing to Alzheimer's disease are provided. In some embodiments, the method comprises determining one or more of the level of expression of rhotekin 2 (RTKN2), the level of expression of microtubule-associated Ser / Thr kinase 4 (MAST4), the level of binding of forkhead box O1 (FOXO1) to the RTKN2 promoter, and the level of binding of amyloid precursor protein (APP) to the MAST4 promoter in a sample from the subject.

This invention relates to compounds which are microtubule associated serine / threonine-like kinase (MASTL) inhibitors and the use of the compounds in the treatment of diseases and medical conditions mediated by MASTL, for example in the treatment of cancer and other target related diseases.

The invention provides construction and application of an intermolecular structural domain tension probe, the probe comprises a first structure, a second structure and a fluorescent protein pair eCFP and eYFP, the first structure and the second structure are respectively connected to two ends of the fluorescent protein pair, and the eCFP and the eYFP are connected through a short peptide; the first structure is a structural domain or protein capable of being combined with a cytoskeleton connecting protein, and the second structure is a structural domain or protein capable of being combined with a cytoskeleton; the cytoskeleton connecting protein is selected from talin, SYNE4, SYNE3, SYNE2, Desmoplakin and the like; the cytoskeleton is a microfilament, a microtubule or an intermediate filament. The intermolecular structural domain tension probe can be used for detecting intracellular mechanical signals, the limitation of a traditional tension probe is overcome, the adverse effect of function enhancement caused by gene cloning is avoided, a new opinion is provided for a mechanical transduction molecular mechanism, and the application has the advantages that the application range is wide, and the application prospect is wide. And a more accurate experimental tool is provided for the research of cell mechanics biology, and the effect of a mechanical signal in cell behavior regulation and control is disclosed.

The invention discloses a composite catchment material of a porous carbon-loaded PEDOT (poly (3, 4-ethylenedioxythiophene)) microtube as well as a preparation method and application of the composite catchment material, and belongs to the field of catchment material preparation. The material comprises porous carbon and a PEDOT (poly (3, 4-ethylenedioxythiophene)) microtube loaded on the surface of the porous carbon, and the PEDOT microtube is poly (3, 4-ethylenedioxythiophene) with a microtube structure. The preparation method of the material comprises the following steps: mixing an oxidizing compound M and a carbon source, and carbonizing to obtain a porous carbon precursor; and carrying out in-situ polymerization reaction on the porous carbon precursor, a 3, 4-ethylenedioxythiophene monomer and acid in a closed environment to obtain the porous carbon material. The composite water collecting material shows efficient, rapid and reversible atmospheric water capturing capability in a wide humidity range, is excellent in cycling stability, and has remarkable engineering practical value and industrialization prospect.

The invention relates to generation, optimization and characterisation of VHH targeted against Tau MTBD (microtubule-binding domain) with high affinity, obtained by screening from a naïve synthetic library. The inventors optimized version of a lead VHH which is able to inhibit Tau aggregation in vitro and in HEK 293 aggregation-reporting cellular model, providing a new tool in Tau immunotherapies. Accordingly the invention relates to new VHH antibody that specifically binds with high affinity Tau species, especially the epitope region involved in Tau aggregation. Moreover, the inventors found that immunization with the optimized version of this lead VHH prevented the formation of neurofibrillary tangles induced by injection of extracellular h-AD in mouse model. Thus, these specific antibodies can be used for the therapy of tauopathy disorders such as Progressive supranuclear palsy (PSP).

The utility model provides a quick -plug formula microtubule reducing joint of sealing structure area belongs to precision instrument technical field. Including the connector, still including the reinforcement subassembly, reinforcement subassembly includes setting up the support pipe of connector one side, the one side of support pipe all is seted up with the sliding slot, the inside of sliding slot is provided with the rolling ball and slides, the one side of support pipe is provided with the connecting soft pad, reinforcement subassembly at the time of use, support pipe plays the basic support effect, sliding slot provides the sliding space for the rolling ball, and the rolling ball can be in the flexible movement in the groove, be used for adjusting position, and the connecting soft pad can enhance the sealing property in the connection, reduce the attrition between parts, thereby make the whole reinforcement subassembly realize the effect of firm connection and good sealing in use, the connecting pipe's fixed groove and the connecting soft pad of support pipe joint, enhance the connection sealing property, the connecting pipe slot place is equipped with the rotating shaft, and the shaft interlocking block can rotate around the shaft, and the spring piece of slot place connects the interlocking block, provides the elasticity.

The present application belongs to the technical field of plant root monitoring, and particularly relates to a method for protecting micro-root tubes from being damaged by rodents, which comprises the following steps: identifying specific positions of the micro-root tubes through micro-root tube data acquisition, image classification training, contour feature extraction, alarm identification and video pre-recording, and taking snapshots; using a key algorithm of frame separation to analyze, so as to realize protection of the micro-root tubes and automatic identification and automatic alarm of the micro-root tubes damaged or lost by rodents; after the micro-root tubes are determined and identified, an aluminum alloy pop-top can with a removed top end is sleeved on an exposed end; since the material of the pop-top can is plastic, after the root system is determined, the pop-top can can be pinched to make the pop-top can more closely attached to the micro-root tube, so as to play a role in protecting the micro-root tube.

The invention provides a myocardial cell fusion automatic arrangement method based on a micro-channel, which comprises the following steps: initializing an operation visual field, and placing the micro-channel, myocardial cells and a microtube orifice in the same visual field; based on the current visual field image, myocardial cell detection and micro-channel and micro-tube orifice identification are carried out on the visual field image shot by the camera by using an image processing technology, and myocardial cells are numbered; determining the initial positions of myocardial cells and microtubule orifices and the target position of the microtubule orifice when the myocardial cells are moved and discharged to the microchannel, and planning the moving path of the microtubule; in order to control the position of the myocardial cells in the liquid which is sucked into or discharged out of the microtubule along with the microtubule, a sliding mode surface is constructed according to a myocardial cell movement model, and a sliding mode active-disturbance-rejection controller is designed to control a stepping motor in the process of transferring the myocardial cells by the microtubule; and according to the numbers of the myocardial cells, moving the myocardial cells in the visual field through the microtubes according to a planned path and discharging the myocardial cells into the micro-channels to finish automatic arrangement.

The present invention provides a compound represented by formula (I), a pharmaceutical composition, and methods for preparing and using the same. The compound has good KIF18A inhibitory activity and can regulate KIF18A protein either alone or by forming a binding complex with microtubules, thereby treating KIF18A-mediated disorders and / or diseases, such as neoplastic diseases, and can be used to prepare drugs for such disorders or diseases. [Formula 1] JPEG2025533411000090.jpg30169

The present invention provides a medicine for treating a neurodegenerative disease, which employs LEFTY2 (Left-Right Determination Factor 2) generated by co-culturing mesenchymal stem cells of mammals with nerve cells having mutations in the APP (Amyloid precursor protein) gene, or a specific protein. The LEFTY2 has an effect of inhibiting Beta amyloid and a hyperphosphorylated neuronal microtubule-associated protein (Tau protein), without affecting the development of nerve cells and having the ability to promote the growth of the nerve cells; and the two types of proteins have a crucial impact on the neurodegenerative diseases.

The invention discloses application of a substance for inhibiting Sqstm1 gene expression and inhibiting Sqstm1 from transmitting phosphoric acid chemical energy to a microtube in an extracellular vesicle secretion inhibitor, and relates to the technical field of biomedicine. The application method comprises the following steps: screening a substance capable of targeting a transcription product of the Sqstm1, an inhibiting substance targeting protein expression of the Sqstm1, and a substance for inhibiting the Sqstm1 from transmitting phosphoric acid chemical energy to a microtubule. The Sqstm1 is used as a key target of the EVs inhibitor as a core, and the main advantage of the Sqstm1 is that the inhibition of the Sqstm1 does not affect the generation of intracellular vesicles. Therefore, the Sqstm1 can be used as a novel vesicle transportation / secretion inhibition target, and a new strategy and thought are provided for scientific research on vesicle transportation / secretion in the future.

The invention belongs to the technical field of medicines, and particularly relates to application of arginine in repairing skeletal muscle injury caused by monensin poisoning. It is found for the first time that arginine can remarkably recover the normal function of skeletal muscle mitochondria and the normal polymerization capacity of microtubules by up-regulating the expression level of mitochondrial oxidative phosphorylation (OXPHOS) protein complex and regulating microtubule polymerization kinetics and reversing monensin-induced skeletal muscle mitochondrial dysfunction and microtubule network disintegration; the method provides a theoretical basis for clinical transformation, and has good practical application value.

The present application provides a compound shown in formula (I), a pharmaceutical composition and a preparation method and application thereof. The compound has good KIF18A inhibition effect, can be combined with microtubules to form a complex to regulate KIF18A protein, is used for treating KIF18A-mediated diseases and / or diseases, such as tumor diseases, and is used for preparing medicines for such diseases or diseases.

This invention relates to compounds which are microtubule associated serine / threonine-like kinase (MASTL) inhibitors and the use of the compounds in the treatment of diseases and medical conditions mediated by MASTL, for example in the treatment of cancer and other target related diseases.

The application discloses a kind of based on super-resolution image's platelet microtubule classification method and classification system, the classification method, including the following steps: obtaining immunofluorescence labeled platelet microtubule super-resolution image;With single platelet microtubule structure distribution as classification object, according to classification principle is divided into "regular distribution", "diffuse distribution", "aggregated distribution" and "irregular distribution" four categories, the classification method can be used to classify platelet microtubule in sample, and it has important significance to platelet physiological function research.In addition, based on the classification method, the platelet microtubule classification system based on super-resolution image provided by the application can automatically extract the complete image of single platelet microtubule based on the obtained platelet microtubule super-resolution image, and mark the type of microtubule according to the classification principle, and automatically display the classification result, which can automatically classify platelet microtubule based on platelet microtubule super-resolution image.

The application discloses a kind of microtubule automation production device, including rack, annular conveying mechanism is rotationally arranged on rack, a plurality of test tube racks for loading microtubule are fixed on annular conveying mechanism, test tube filling mechanism, spraying mechanism, drying mechanism, cap filling mechanism, cap pressing mechanism, cap presence / absence detection mechanism, cap pressing detection mechanism, rejection mechanism and tray loading mechanism are sequentially provided along the conveying path of annular conveying mechanism, and test tube filling mechanism is adjacently arranged with tray loading mechanism.The microtubule automation production device of the application can automatically complete a series of work such as feeding, drying assembly and automatic tray loading of microtubule, the whole process has high degree of automation, the device structure design is reasonable, operation accuracy is high, efficiency is high, can greatly improve the production efficiency of microtubule, save manpower and material resources, reduce production cost.

The present application provides a compound shown in formula (I), a pharmaceutical composition and a preparation method and application thereof. The compound has good KIF18A inhibition effect, can be combined with microtubules to form a complex to regulate KIF18A protein, is used for treating KIF18A-mediated diseases and / or diseases, such as tumor diseases, and is used for preparing medicines for such diseases or diseases.

The application discloses a kind of nuclear targeting peptide modified DNA nanoparticles based on microfluidic technology and its preparation method and application, it is related to biological medicine technical field, including poly (β- amino ester) cationic polymer as gene delivery carrier, microtubule associated sequence MTAS and nuclear localization signal NLS short peptide are included on the gene delivery carrier, the gene delivery carrier is mixed with plasmid DNA carrying target gene to prepare into nanoparticle, the plasmid DNA carrying target gene is uniformly dispersed in nanoparticle using microfluidic mixing process;The nuclear targeting peptide modified DNA nanoparticles of the application structure design is ingenious, using microfluidic mixing process improves the uniformity of nanoparticle carrier and plasmid DNA mixing, the performance of nanoparticle is further improved by optimizing dialysis condition, reduces potential cytotoxicity and adverse effect, can produce strong tumor killing capacity and cytokine secretion after transducing non-activated T cell.