11 results about "Microtubule" patented technology

The application provides a construction of a fluorescent protein labeled microtubulin and a microtubule binding protein universal bivalent carrier, and the process is as follows: a first stage is to construct a GFP-alpha tubulin carrier, and a second stage is to construct a GFP-alpha tubulin-mCherry universal bivalent co-expression carrier; in the second stage, 35S, mCherry and NOS sequences are inserted into a KpnI enzyme cutting site of a multiple cloning site of the GFP-alpha tubulin carrier constructed in the first stage, and a single nucleic acid enzyme cutting site is inserted into the 5' end and the 3' end of the mCherry sequence, respectively, and the single nucleic acid enzyme cutting site is XbaI, KpnI / Acc65I and AscI sequences, respectively, so as to obtain the universal bivalent carrier. The universal bivalent carrier provided by the application can express two target genes simultaneously, has the fluorescent signals of GFP and mCherry, can quickly and accurately identify a transgenic plant, is convenient for positive seedling screening, can be used for a tobacco transient expression experiment, can shorten an experimental period, and saves time and effort.

The present disclosure relates to polynucleotides and methods of inhibiting the activities of development-associated pathways, including the Wnt, Hedgehog, and Notch pathways, and the invasive capacity of malignant cells to treat primary or secondary solid tumors.

The application discloses a kind of porous carbon load PEDOT microtubule composite water collection materials and preparation method and application thereof, belong to water collection material preparation field.The material includes porous carbon and its surface load PEDOT microtubule, and the PEDOT microtubule is poly 3,4-ethylenedioxythiophene with microtubule structure.The preparation of the material is that oxidizing compound M and carbon source are mixed, carbonization is carried out to obtain porous carbon precursor;Again, the porous carbon precursor is polymerized with 3,4-ethylenedioxythiophene monomer and acid in closed environment to obtain.The composite water collection material of the application shows efficient, fast, reversible atmospheric water capture capacity in wide humidity range, and excellent cycle stability, with significant engineering practical value and industrialization prospect.

The invention discloses a composite catchment material of a porous carbon-loaded PEDOT (poly (3, 4-ethylenedioxythiophene)) microtube as well as a preparation method and application of the composite catchment material, and belongs to the field of catchment material preparation. The material comprises porous carbon and a PEDOT (poly (3, 4-ethylenedioxythiophene)) microtube loaded on the surface of the porous carbon, and the PEDOT microtube is poly (3, 4-ethylenedioxythiophene) with a microtube structure. The preparation method of the material comprises the following steps: mixing an oxidizing compound M and a carbon source, and carbonizing to obtain a porous carbon precursor; and carrying out in-situ polymerization reaction on the porous carbon precursor, a 3, 4-ethylenedioxythiophene monomer and acid in a closed environment to obtain the porous carbon material. The composite water collecting material shows efficient, rapid and reversible atmospheric water capturing capability in a wide humidity range, is excellent in cycling stability, and has remarkable engineering practical value and industrialization prospect.

The utility model provides a quick -plug formula microtubule reducing joint of sealing structure area belongs to precision instrument technical field. Including the connector, still including the reinforcement subassembly, reinforcement subassembly includes setting up the support pipe of connector one side, the one side of support pipe all is seted up with the sliding slot, the inside of sliding slot is provided with the rolling ball and slides, the one side of support pipe is provided with the connecting soft pad, reinforcement subassembly at the time of use, support pipe plays the basic support effect, sliding slot provides the sliding space for the rolling ball, and the rolling ball can be in the flexible movement in the groove, be used for adjusting position, and the connecting soft pad can enhance the sealing property in the connection, reduce the attrition between parts, thereby make the whole reinforcement subassembly realize the effect of firm connection and good sealing in use, the connecting pipe's fixed groove and the connecting soft pad of support pipe joint, enhance the connection sealing property, the connecting pipe slot place is equipped with the rotating shaft, and the shaft interlocking block can rotate around the shaft, and the spring piece of slot place connects the interlocking block, provides the elasticity.

The present invention provides a medicine for treating a neurodegenerative disease, which employs LEFTY2 (Left-Right Determination Factor 2) generated by co-culturing mesenchymal stem cells of mammals with nerve cells having mutations in the APP (Amyloid precursor protein) gene, or a specific protein. The LEFTY2 has an effect of inhibiting Beta amyloid and a hyperphosphorylated neuronal microtubule-associated protein (Tau protein), without affecting the development of nerve cells and having the ability to promote the growth of the nerve cells; and the two types of proteins have a crucial impact on the neurodegenerative diseases.

The invention discloses application of a substance for inhibiting Sqstm1 gene expression and inhibiting Sqstm1 from transmitting phosphoric acid chemical energy to a microtube in an extracellular vesicle secretion inhibitor, and relates to the technical field of biomedicine. The application method comprises the following steps: screening a substance capable of targeting a transcription product of the Sqstm1, an inhibiting substance targeting protein expression of the Sqstm1, and a substance for inhibiting the Sqstm1 from transmitting phosphoric acid chemical energy to a microtubule. The Sqstm1 is used as a key target of the EVs inhibitor as a core, and the main advantage of the Sqstm1 is that the inhibition of the Sqstm1 does not affect the generation of intracellular vesicles. Therefore, the Sqstm1 can be used as a novel vesicle transportation / secretion inhibition target, and a new strategy and thought are provided for scientific research on vesicle transportation / secretion in the future.

The invention belongs to the technical field of medicines, and particularly relates to application of arginine in repairing skeletal muscle injury caused by monensin poisoning. It is found for the first time that arginine can remarkably recover the normal function of skeletal muscle mitochondria and the normal polymerization capacity of microtubules by up-regulating the expression level of mitochondrial oxidative phosphorylation (OXPHOS) protein complex and regulating microtubule polymerization kinetics and reversing monensin-induced skeletal muscle mitochondrial dysfunction and microtubule network disintegration; the method provides a theoretical basis for clinical transformation, and has good practical application value.

The present application provides a compound shown in formula (I), a pharmaceutical composition and a preparation method and application thereof. The compound has good KIF18A inhibition effect, can be combined with microtubules to form a complex to regulate KIF18A protein, is used for treating KIF18A-mediated diseases and / or diseases, such as tumor diseases, and is used for preparing medicines for such diseases or diseases.

The present application provides a compound shown in formula (I), a pharmaceutical composition and a preparation method and application thereof. The compound has good KIF18A inhibition effect, can be combined with microtubules to form a complex to regulate KIF18A protein, is used for treating KIF18A-mediated diseases and / or diseases, such as tumor diseases, and is used for preparing medicines for such diseases or diseases.

The application discloses a kind of nuclear targeting peptide modified DNA nanoparticles based on microfluidic technology and its preparation method and application, it is related to biological medicine technical field, including poly (β- amino ester) cationic polymer as gene delivery carrier, microtubule associated sequence MTAS and nuclear localization signal NLS short peptide are included on the gene delivery carrier, the gene delivery carrier is mixed with plasmid DNA carrying target gene to prepare into nanoparticle, the plasmid DNA carrying target gene is uniformly dispersed in nanoparticle using microfluidic mixing process;The nuclear targeting peptide modified DNA nanoparticles of the application structure design is ingenious, using microfluidic mixing process improves the uniformity of nanoparticle carrier and plasmid DNA mixing, the performance of nanoparticle is further improved by optimizing dialysis condition, reduces potential cytotoxicity and adverse effect, can produce strong tumor killing capacity and cytokine secretion after transducing non-activated T cell.