Method for disposable measuring bighead atractylodes rhizome inner ester and atractylone with HPLC microtubule method disposable

A one-time technology for the determination of atractylodes lactone and atractylodes ketone, and the one-time determination of atractylodes atractylodes lactone and atractylodes ketone by HPLC microtube method, which can solve the problems of time-consuming and laborious, and increase the error of the three components. Achieve the effect of simplifying operation and reducing errors

Inactive Publication Date: 2008-01-30
ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the disadvantage of the content determination method of a single component is that two test conditions are required, and the determination is performed twice, which is not only time-consuming and laborious, but also increases the error of the internal ratio of the three components.
[0004] In view of the fact that there is no determination method and standard for the content of volatile oil, atractylone and atractylodes lactone in the "Pharmacopoeia of the People's Republic of China (2005 Edition, Part One)", the same chromatographic conditions can be used for the one-time determination of Atractylodes atractylodes III and Atractylodes atractyloides in Atractylodes macrocephala. The method of I and atractylone has not been reported

Method used

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  • Method for disposable measuring bighead atractylodes rhizome inner ester and atractylone with HPLC microtubule method disposable
  • Method for disposable measuring bighead atractylodes rhizome inner ester and atractylone with HPLC microtubule method disposable
  • Method for disposable measuring bighead atractylodes rhizome inner ester and atractylone with HPLC microtubule method disposable

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1: Determination of the three components of Jiangxi Anfu Atractylodes Rhizome sample

[0034] (1) Preparation of mobile phase

[0035] Get chromatographically pure methanol, acetonitrile and redistilled water, and prepare mobile phase A and B solutions by volume ratio

[0036] Mobile phase A solution: methanol: acetonitrile: water = 90:5:5

[0037] Mobile phase B solution: methanol: acetonitrile: water = 55:5:40

[0038] (2) Preparation of reference substance solution

[0039] Accurately weigh 5.8 mg of Atractylodes lactone I, 6 mg of Atractylodes macrolide III and 6.3 mg of atractylodes ketone on an analytical balance, put them into 10ml measuring bottles respectively, add methanol to the 10ml scale, shake well to dissolve the reference substance; then absorb Atractylodes atractylodes Add 10 μl each of lactone I (0.58 mg / ml), Atractylodes lactone III (0.6 mg / ml), 80 μl of atractylone (0.63 mg / ml) solution, add 900 μl of mobile phase B solution, and mix well ...

Embodiment 2

[0057] Example 2: Determination of content of three components in Shaodong Atractylodes macrocephala sample

[0058] (1) Preparation of mobile phase

[0059] Get chromatographically pure methanol, acetonitrile and redistilled water, and prepare mobile phase A and B solutions by volume ratio

[0060] Mobile phase A solution: methanol: acetonitrile: water = 90:5:5

[0061] Mobile phase B solution: methanol: acetonitrile: water = 55:5:40

[0062] (2) Preparation of reference substance solution

[0063] Accurately weigh 5.8 mg of Atractylodes lactone I, 6 mg of Atractylodes lactone III, and 6.3 mg of atractylodes ketone on an analytical balance, put them into 10ml measuring bottles respectively, add methanol to the 10ml mark, shake well to dissolve the reference substance, and then absorb Atractylodes atractylodes Ester I (0.58mg / ml), Atractylodes lactone III (0.6mg / ml) each 10μl, atractylone (0.63mg / ml) solution 80μl, add mobile phase B solution 900μl, mix to obtain Atractylodes...

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Abstract

The invention discloses a method of single time measurement of atractylenolide and atractylon. The procedure includes preparing mobile phase resolution A and B made of chromatographic pure methanol, acetonitrile and redistilled water, preparing the mixed control solution made of atractylenolide I, atractylenolide III and atractylon, preparing sample solution, testing the three absorption peaks of control solution and samples at three different time respectively by chromatograph of liquid according to the setting gradient of mobile phase A and B and the time, and calculating the content of atractylenolide I, atractylenolide III and atractylon according to the formula X=(peak area of sample is multiplied by density of control then is divided by peak area of control and is multiplied by density of sample) is multiplied by 100 percent. The invention can determine the content of atractylenolide I, atractylenolide III and atractylon in atractylodes macrocephala by only one sample injection. Thus the operation is simplified, the error of multiple sample injection is reduced and analysis of the constituent ration of the three ingredients is convenient. The invention is of important academic value and meaningful in practical application.

Description

technical field [0001] The invention relates to a method for determining atractylactone and atractylone, in particular to a method for one-time determination of atractylactone and atractylone by using an HPLC microtube method. The invention belongs to the technical field of determination of traditional Chinese medicine components. Background technique [0002] The traditional Chinese medicine Atractylodes macrocephala is the dried rhizome of Atractylodes macrocephala (Rhizoma atractylodis macrocephala Koidz.) "Pharmacopoeia of the People's Republic of China", 2005 edition, part one. It is a well-known traditional multi-purpose commonly used traditional Chinese medicine, and is one of the "Eight Flavors of Zhejiang". It has the effects of invigorating the spleen and replenishing qi, drying dampness and diuresis, antiperspirant and antiabortive. It is planted in many provinces in China, and Zhejiang Province is one of the main producing provinces. The annual output of our pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N33/15G06F19/00
Inventor 戴诗文章建民寿旦
Owner ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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