The invention relates to the field of
traditional Chinese medicine detection technology, in particular to a characteristic spectrum construction method and a quality detection method for bighead atractylodes rhizomes. According to the characteristic spectrum construction method for bighead atractylodes rhizomes, a characteristic spectrum of effective ingredients, namely
atractylenolide I,
atractylenolide II,
atractylenolide III and atractylon of bighead atractylodes
rhizome medicinal materials, bighead atractylodes
rhizome herbal pieces and bighead atractylodes
rhizome herbal pieces stir-bakedwith
bran are analyzed through efficient liquid chromatographic analysis, chromatographic peaks of the characteristic spectrum are completely displayed, uniformity is good, the characteristic chromatographic peaks are completely separated, the quantity of impurities is small, a contrast characteristic spectrum is established according to the characteristic spectrum, and meanwhile the content of
atractylenolide I and the content of
atractylenolide III are determined. In combination with thin-layer
chromatography identification and heavy
metal and harmful element determination, the comprehensive quality detection method for bighead atractylodes rhizomes with effectiveness and safety is provided, so that quality detection on bighead atractylodes rhizomes, processed products of bighead atractylodes rhizomes and relevant products is realized. Moreover, the quality detection method has the advantages of being simple, fast, stable, reliable, high in precision, good in reproducibility, easy to grasp and the like.