78 results about "Chicoric acid" patented technology

The present invention relates to process of extracting high purity chicoric acid from Echinacea purpurea. The process includes the following steps: 1. extracting crushed Echinacea purpurea root with alcohol and separating to eliminate solid impurity; 2. pre-separating the extracted liquid after recovering alcohol in a macroporous resin column through eluting with alcohol solution of different concentrations to obtain the extract; and 3. purifying the extract through one high speed counter current chromatographic process to obtain chicoric acid of purity over 99 %.

The process of preparing chicoric acid from echinacea purpurea includes organic solvent extraction, acidification, solvent extraction, resin separation, crystal in solvent and other technological steps. During organic solvent extraction, water solution of ethanol of 30-50 wt% concentration and in the amount of 4-10 times echinacea purpurea weight is used as the organic solvent to prepare water solution of chicoric acid at the temperature of 70-90 deg.c. During the acidification of water solution of chicoric acid, the pH value of the water solution of chicoric acid is regulated with acid solution to not greater than 3. The separation and purification process includes separation with macroporous resin, washing with deionized water, elution with ethanol aqua, concentration to obtain coarse chicoric acid product, dissolving the coarse chicoric acid product in water, pH regulation of the chicoric acid aqua to 1-3 and cooling to crystallize and to obtain chicoric acid product with purity over 96 %.

The invention relates to an echinacea extract and a preparation method of the echinacea extract. The echinacea extract provided by the invention is obtained through carrying out twice extraction on echinacea medicinal materials by alkaline ethanol solution and lysozyme; and in the product, the polysaccharide content is greater than or equal to 21.50 percent, the polyphenol content is greater than or equal to 8.10 percent, and the chicoric acid content is greater than equal to 5.00 percent. Because the polyphenol content and the chicoric acid content of the echinacea extract are obviously increased, the echinacea extract is prepared into ordinary preparations including powder or oral liquid and the like, and the curative effect is greatly improved. In a compound preparation processed by the echinacea extract and bacteriolysant according to a consumption ratio being 3:1, active ingredients of echinacea and lysozyme totally account for 20 percent, wherein the consumption ratio of the echinacea extract to the lysozyme is (4-1):1, auxiliary materials of cane sugar account for 64 percent, and dextrin accounts for 16 percent. The compound preparation has the functions of improving the immunity of live stock and realizing the sterilization and antivirus functions, and can be used as medicine for preventing and treating animal diseases, particularly chicken diseases caused by escherichia coli infection.

A process for preparing high-purity chicoric acid from chicory includes pretreating to increase the content of chicoric acid in chicory and preventing the decomposition of chiconic acid, choosing proper solvent and separating technique for extracting the active component to maximum, choosing purifying technique and equipment for removing impurities, choosing optimized separating carrier and filler, and choosing proper product form.

The invention discloses a method for extracting refined chicoric acid. According to the method, echinacea raw materials are treated by steps of extracting by ultrasonic waves, decompressing and concentrating in vacuum, sampling macroporous resin, washing with de-ionized water, eluting, decompressing and concentrating in vacuum, spraying and drying, detecting, crushing, and packaging and storing. In the extraction process of the chicoric acid, ultrasonic wave assisting is utilized to improve the extraction rate of the chicoric acid; the problems that the extraction time in the extraction process is long and the extraction rate is low are effectively improved; a macroporous resin adsorption technology is used for removing impurities in an alcohol extracting solution and effective components are enriched; the problems that in the prior art, the impurity removing rate is low, the loss of effective components is great, the purity of later-period products is low and the like are solved.

The present invention discloses an echinacea purpurea extract. The echinacea purpurea extract comprises 2.4 to 3.1% of caftaric acid, 4.4 to 5.7% of chicoric acid and 1.5 to 2.0% of echinacoside based on the weight percentages of all ingredients accounting for the dry weight of the extract. The present invention also discloses a preparing method of echinacea purpurea extract and a detection method for phenols ingredient content in echinacea purpurea extract. The phenols ingredient content in the echinacea purpurea extract all meet the requirements of the active ingredient content specification of echinacea purpurea in United State Pharmacopeia 30 (USP 30). The phenols ingredient content is increased by 4 to 6% than that of echinacea purpurea extract sold on market. The preparing method has the advantages of simple technique, low cost, low energy consumption and easy industrialization.

The invention relates to an echinacea extract product, a preparation method and an application, the extract product comprises the following components by weight percentage: content of phenolic compounds is higher than 12%, content of Echinacea polysaccharide is higher than 20%, and chicoric acid content is higher than 4%. The extract product has obvious promotion effect to the generation of mice hemolysin antibody, the echinacea extract product can enhance humoral immunity, and the effect of the echinacea extract product is better than that of the prior art.

The invention discloses a purifying method for chicoric acid in echinacea purpurea. The method comprises the following steps that 1, an echinacea extract is gotten and used for preparing a sample solution with the chicoric acid content being 1.3-5 mg/mL, the pH value of the sample solution is adjusted to 2-5, and then the sample solution serves as a sample loading solution; 2, packing is conducted through a macroporous resin wet method, and the sample loading solution is used for sample loading, wherein the sample loading speed ranges from 2 Bv/h to 3 Bv/h, and the adsorption time ranges from 3 hours to 5 hours; 3, eluting is conducted through deionized water of 0.5-2 Bv, and then ethyl alcohol of 10%-30% is used as an eluting solvent for eluting, wherein the eluting speed ranges from 1 Bv/h to 3 Bv/h; ethyl alcohol eluent of 0.25 Bv to 2.25 Bv is collected and volatilized, and then a chicoric acid product is obtained. By means of the purifying method, the operating steps are simple, no poisonous chemical reagent is used, the purity of the chicoric acid is increased to 85% from 4% through one-time treatment, the number of the purification folds is 21 or more, and the recovery rate is 77% or so.

The invention discloses a new compound -iso chicoric acid, which comprises the following steps: water-extracting alcohol deposition for embrace stem gutweed vegetable; centrifuging; adding burnt lime into supernatant fluid; depositing with pH value at 9-12; centrifuging; depositing; adding into alcohol to float; adding into sulfuric acid to adjust pH value at 1-4; centrifuging; reclaiming alcohol; proceeding polyamide or macroreticular resinous column chromatography; washing; eluting with diverse density alcohol; collecting eluent of each section; condensing; disposing with acetone, acetic ester and methyl alcohol; recrystallizing repeatedly; getting the product. This invention also discloses usages of iso chicoric acid.

The invention relates to a synthesis method of (-)-chicoric acid, which comprises the following steps: reacting diacetylcaffeic acid with (L)-tartaric acid in the presence of thionyl chloride or oxalyl chloride to obtain (-)-tetraacetylchicoric acid; or reacting diacetylcaffeic acid with thionyl chloride to obtain diacetyl caffeic acyl chloride, and reacting with (L)-tartaric acid to obtain the (-)-tetraacetylchicoric acid; and finally, removing acetyl group in the (-)-tetraacetylchicoric acid in a mixed solution of an acid solution and a water-soluble organic solvent, thereby obtaining the (-)-chicoric acid. By removing the steps of tartaric acid carboxyl protection and deprotection, the invention has the advantage of simple synthesis technique, and is economical and practical.

The invention discloses an application of a chicoric acid extract in echinacea purpurea, namely, an application of the chicoric acid extract as an edible fat and oil antioxidant. A purified chicoric acid extract extracted from the echinacea purpurea is added in edible fat and oil according to a certain dosage, and the antioxygenation function of the chicoric acid extract of the echinacea purpurea is simultaneously achieved by using three methods of measuring acid value (AV), peroxidation value (POV) and thiobarbituric acid (TBA) of fat and oil, results prove that the chicoric acid extract in the echinacea purpurea has a remarkable antioxygenation effect to lard oil and colleseed oil, can effectively delay the lipid peroxidation of the fat and oil, and can be applied to the food industry.

The invention provides a compound coneflower preparation, wherein a use ratio of effective components such as a coneflower extract and lysozyme is 3:1, the total content of the coneflower extract and the lysozyme is 25%, and contents of auxiliary materials such as sucrose and dextrin are respectively 60% and 15%. According to the present invention, the selected coneflower extract is obtained by extracting a coneflower herb with an alkaline ethanol solution and lysozyme; the polysaccharide content exceeds 20%, the polyphenol content exceeds 8%, and the chicoric acid content exceeds 5%; and the composite preparation has functions of animal immunity improving, anti-bacterial and anti-virus, and can be used for preparation of drugs for prevention and treatment of animal diseases, especially prevention and treatment of chicken diseases caused by escherichia coli infection.

The invention discloses a compound echinacea purpurea preparation. The dose ratio of an echinacea purpurea extract as an effective component to lysozyme is (4-1):1, the dosage of the echinacea purpurea extract and the lysozyme is 20% in total, and the ajudvants contains 64% of saccharose and 16% of dextrin. The echinacea purpurea extract selected by the invention is obtained by extracting echinacea purpurea medicinal material by alkaline ethanol solution and lysozyme for two times, wherein the content of polysaccharide is not less than 21.5%, the content of polyphenol is not less than 8.10% and the content of chicoric acid is not less than 5.00%. The compound preparation provided by the invention has the effects of improving animal immunity, antibiosis and anti-virus, thereby being capable of being taken as the medicine for preventing and treating the animal diseases, in particular to the chicken diseases caused by escherichia coli infections.

The invention relates to a medicinal application of chicoric acid and application of anti-tumor medicaments and health products formed by chicoric acid. In-vitro tests prove that chicoric acid has the effect of a histone deacetylase (HDAC) inhibitor.

The invention provides a method for extracting chicoric acid from echinacea purpurea moench. The method comprises the following steps: 1) extracting the echinacea purpurea moench by 60 to 75v% ethyl alcohol, filtering an extracting solution, and taking filtrate for standby; 2) adding activated carbon into the filtrate to carry out decoloration, and filtering to obtain supernatant liquid; 3) after the supernatant liquid is concentrated, putting a silica gel column to carry out chromatography, washing the column with water until the column is colorless, then, carrying out elution by a mixed solvent of methyl alcohol and acetone, and collecting a eluent of the methyl alcohol and the acetone; 4) carrying out the chromatography on the eluent of the methyl alcohol and the acetone on the silica gel column again, eluting by the mixed solvent of the ethyl alcohol, the acetone and water, collecting the eluent of the ethyl alcohol, the acetone and the water, recovering the solvent, and drying to obtain the chicoric acid. According to the method, the yield and the purity of the chicoric acid can be greatly improved, the yield of an echinacea purpurea moench dried product can reach not less than 1.50% through detection, and purity can reach not less than 96.5%.

The invention relates to the field of analysis of traditional Chinese medicine components, and relates to QAMS for detecting the content of organic acids in sowthistle-leaf ixeris seedling injection. The QAMS disclosed by the invention comprises the following steps: (1), setting chromatographic conditions; (2), preparing a reference solution; (3), preparing a test solution; and (4), calculating correction factors. Relative correction factors of chicoric acid, mono-caffeoyl tartaric acid, chlorogenic acid and caffeic acid are calculated by taking chicoric acid as the internal reference compound, and used as constants for detecting the content; correction factors are calculated by taking effective active components in the sowthistle-leaf ixeris seedling injection and high-content chicoric acid as internal reference compounds; the content of mono-caffeoyl tartaric acid, chlorogenic acid and caffeic acid in the sowthistle-leaf ixeris seedling injection are calculated; the content of the four organic acids in the injection can be detected simultaneously; therefore, the quality of the sowthistle-leaf ixeris seedling injection in industrial production can be monitored scientifically, sensitively and rapidly; and simultaneously, the problem that the quality of the sowthistle-leaf ixeris seedling injection cannot be controlled objectively and reasonably because of shortage of reference substances can be solved.

The invention relates to a separating preparation method of phenolic acid compounds in Echinacea purpurea. The preparation method comprises the steps: performing segment enrichment of the phenolic acid compounds in Echinacea purpurea firstly by using the property that the elution rate can be accelerated by an eluent in pH zone countercurrent chromatography, and then using the pH zone countercurrent chromatography separation on Echinacea purpurea so as to obtain eight high-purity phenolic acid compounds including caftaric acid, fertaric acid, coutaric acid, iso-fertaric acid, methyl caffeoyltartrate, chicoric acid (2,3-O-dicaffeoyltartaric acid), ferulic acid and methyl caffeate. Through the method, not only can a high injection volume be ensured, but also lower-content compounds are enriched after segment enrichment is performed, so that more compounds are obtained during second separation, and the separation efficiency is improved greatly.

The invention discloses a method for simultaneously extracting chicoric acid, mono-caftaric acid, 3,5-dicaffeoylquinic acid and chlorogenic acid from pterocypsela indica as a raw material. After pterocypsela indica is extracted by a solvent (or in assistance of ultrasonic wave and microwave), the maximum extraction amounts of the four components, namely chicoric acid, mono-caftaric acid, 3,5-dicaffeoylquinic acid and chlorogenic acid, can respectively reach 25.3mg/g, 3.62mg/g, 6.0mg/g and 3.6mg/g, wherein the content of chicoric acid is the highest. The pterocypsela indica can serve as the raw material from which the natural products can be extracted. According to the method, the pterocypsela indica is effectively utilized; the pterocypsela indica as weeds to be removed becomes to a raw material which can be initiatively utilized; a scientific basis is provided for reasonably utilizing the pterocypsela indica; the method has the characteristics of rich sources and green processes.

The invention provides a fetal bovine marrow tablet and a production method thereof. The fetal bovine marrow tablet solves the problem that the existing fetal bovine marrow tablet has a low active substance utilization rate. The fetal bovine marrow tablet is prepared from 1.1-12.7 parts by weight of bone peptide, 0.5-7.6 parts by weight of fetal bovine marrow, 5.5-41.4 parts by weight of zymolytic bone calcium powder, 10.5-70.4 parts by weight of chrysanthemum powder, 0.01-0.1 parts by weight of zinc lactate, 0.2-2 parts by weight of magnesium stearate, 0.1-1.4 parts by weight of silica, 2.6-30.8 parts by weight of calcium carbonate, 2.1-25.5 parts by weight of beer yeast, 0.8-20.1 parts by weight of selenium-enriched yeast, 2.8-45.5 parts by weight of corn starch and 1-5 parts by weight of ethanol with a concentration of 50%. Chicoric acid in chrysanthemum prevents collagen from being influenced by degradation free radical. Through combination of the chrysanthemum powder and fetal bovine marrow, polypeptide in the fetal bovine marrow still keeps activity and has a high utilization rate. The production method has simple processes and a low cost.

Echinacea preparations having standardized chicoric acid levels and inducing an augmented level of immune-stimulatory activity. Augmentation of the immune stimulatory activity is achieved by the selection of a harvest time for Echinacea plants that is during a plant maturation stage that is prior to full bloom. Methods for augmenting the immune-stimulatory effects of Echinacea extracts by harvesting Echinacea plants prior to full bloom.

The invention discloses application of chicoric acid in preparation of a medicament or a health product for treating obesity or complications thereof. The invention expands the application range of the chicoric acid, and the chicoric acid can effectively regulate the body weight and the blood fat level of a high-fat model mouse, improve the kidney function and reduce renal tubular injury.

The invention relates to a preparation method for extracting the high-purity chicoric acid from echinacea purpurea herb. The method is characterized by comprising the steps of preparing a modified glucoside enzyme solution; 2) crushing, filtering and pre-processing a dried echinacea root, and then adding an ethanol aqueous solution to obtain an echinacea root ethanol dispersion solution; 3) putting an erlenmeyer flask containing the ethanol aqueous solution to obtain an echinacea root ethanol dispersion solution into a microwave extraction apparatus, adding the modified glucoside enzyme solution, and conducting the microwave heating process for 30 to 90 seconds; 4) after the microwave enzymatic hydrolysis process, subjecting an obtained enzymatic hydrolysate to the high-temperature enzyme deactivation treatment for 10 to 30 minutes to obtain a chicoric acid primary extraction liquid; 5) filtering and concentrating in the vacuum state to obtain the chicoric acid. According to the invention, the purity of the obtained chicoric acid product is larger than 95%, and the yield thereof is larger than 1.2%. Meanwhile, the extraction yield is high.

The invention discloses a novel application of chicoric acid, namely an application of chicoric acid in preparing a drug for preventing respiratory syncytial virus (RSV). The inventor of the invention screens antiviral components in an echinacea plant by applying a traditional Chinese medicine chemical method and finds the optimum antiviral active ingredient, chicoric acid, through an in vitro antiviral pharmacological experiment and finds that the in vitro antiviral activity of chicoric acid on RSV is the strongest, and is superior to that of a reference drug, ribavirin, so that chicoric acid can be used for preparing the drug for preventing RSV as an active ingredient. In specific application, a pharmaceutically acceptable matrix or auxiliary material can be properly added to chicoric acid, and chicoric acid can be also combined with proper drugs in application.

The invention discloses a method for separating chicoric acid from echinacea, and belongs to the technical field of medicine and chemical engineering. Chicoric acid is a very significant immunity active ingredient in chicory and echinacea. The invention provides an extraction technology which not only can solve problems in extraction and separation, but also provides a recrystallization method, so as to enable the product to be high in purity, and provide a reference for industrial generation.