36 results about "Chicoric acid" patented technology

The invention belongs to the field of biological medicine, and discloses a chicoric acid (CA)-loaded tetrahedral framework nucleic acid (tFNA) nano preparation for treating acute gouty arthritis. According to the preparation, tFNA formed by self-assembly is taken as a carrier, and a natural active ingredient CA is loaded to form a CA-tFNA compound. The compound overcomes the defects that CA is poor in water solubility and low in bioavailability, and existing drugs are weak in targeting and large in side effect. Through the targeted delivery and synergistic effect of tFNA, the preparation can be effectively enriched in inflammatory joints, significantly inhibit NF-kappa B signal pathways, and down-regulate the expression of key proinflammatory factors such as TNF-alpha and IL-1beta, so that joint swelling and synovial tissue damage are relieved, and repair is promoted. Experiments show that the traditional Chinese medicine composition is remarkable in curative effect and good in safety, and a novel efficient treatment strategy is provided for acute gouty arthritis.

The application discloses a dandelion medicinal material effective component rapid determination method based on color chroma values, and relates to the technical field of traditional Chinese medicinal material quality detection. * , a * , b * Chroma values of dandelion medicinal material powder are collected, and the contents of chicoric acid, monocaffeoyl tartaric acid and chlorogenic acid are determined by using high performance liquid chromatography as true values; subsequently, a neural network prediction model with chroma values as input and effective component contents as output is constructed and trained, preferably a single hidden layer structure; finally, the model is used for fast and nondestructive content prediction of unknown samples; compared with a traditional multiple linear regression model, the neural network model used in the application has significantly higher prediction accuracy, and the test set R² is all more than 0.86; the application realizes fast, nondestructive, low-cost and high-precision detection of dandelion medicinal material effective components, and is suitable for multiple scenes such as raw material quality control, market supervision, optimal harvest period judgment and fast pricing grading.

The invention discloses a chicoric acid carrier with a DNA regular tetrahedron as a skeleton and a preparation method of the chicoric acid carrier. According to the method, four oligonucleotide chains with specific sequences are synthesized, so that the oligonucleotide chains are self-assembled in a buffer solution to form a stable DNA regular tetrahedral structure. And then loading the natural active component chicoric acid with poor water solubility and low chemical stability into the interior or on the surface of the tetrahedron in a physical embedding or electrostatic adsorption manner. The obtained compound utilizes a hydrophilic skeleton of a DNA tetrahedron, so that the water solubility and chemical stability of the chicoric acid are remarkably improved, and the degradation rate of the chicoric acid under light, heat and oxidation conditions is effectively reduced. The problem that the clinical application of the chicoric acid is limited due to poor physicochemical properties, fast in-vivo metabolism and difficult targeted delivery is solved, and a new strategy is provided for further development of the chicoric acid.

The invention discloses a tissue culture detoxification method of Amomum tsao-ko, which comprises the following steps: S1, selecting an Amomum tsao-ko plant which grows robustly, taking a tender stem segment of the Amomum tsao-ko plant as an explant, disinfecting, inoculating into a callus induction culture medium, culturing, and inducing the generation of callus; wherein the callus induction culture medium is prepared from 0.8 mg / L to 1.2 mg / L of MS + 2, 4-D, 0.4 mg / L to 0.6 mg / L of 6-BA and 3 mu mol / L to 5 mu mol / L of chicoric acid; s2, heat treatment: cutting the callus obtained in the step S1 into small pieces, and putting the small pieces into sterile water at 40 + / -2 DEG C for heat bath for 15-20 minutes; s3, differentiation culture: inoculating the callus subjected to the hot bath treatment into a differentiation culture medium, and inducing tissue differentiation; wherein the differential culture medium is prepared from 1.2 mg / L to 1.8 mg / L of 1 / 2 MS, 6-BA and 0.04 mg / L to 0.06 mg / L of TDZ; s4, inducing rooting: cutting cluster buds obtained through differentiation culture into single buds without callus tissues, and inoculating the single buds into a rooting culture medium; wherein the rooting culture medium is prepared from 1 / 2 MS, 1.0 mg / L to 1.4 mg / L of IBA, 0.2 mg / L to 0.4 mg / L of NAA and 0.04 mg / L to 0.07 mg / L of brassinolide. By using the method provided by the invention, the tomato spotted wilf virus detoxification success rate in the tsaoko amomum fruit tissue culture process can be effectively improved.

According to the application of the implant-derived exosome as the carrier in preparation of the medicine for targeted therapy of colitis, chicoric acid is loaded in the purple rice exosome, so that the defects that chicoric acid is low in oral bioavailability and poor in gastrointestinal tract stability are overcome, colon targeting and accumulation of the medicine are achieved, and the curative effect of the medicine is improved. An ideal functional carrier and a candidate preparation are provided for developing a novel anti-colitis nano-drug with high efficiency and low toxicity, and an innovative path is opened up for deep processing and industrial chain extension of purple rice resources in Yunnan province.

Provided herein are polyphenol-enriched lettuce powders comprising particles of a polyphenol-enriched lettuce or extract, wherein the polyphenol-enriched lettuce comprises from 40-280 mg / g of polyphenols. The polyphenols include any combination of a chlorogenic acid, a neochlorogenic acid, a chicoric acid, a quercetin, a quercetin derivative, and an anthocyanin. The disclosure also provides compositions and methods of using the polyphenol-enriched lettuce powders to promote wound healing and treat inflammation and skin disorders.

The application provides a method for detecting the pharmacodynamic components of a traditional Chinese medicine composition by UPLC-MS / MS. The method can identify 14 pharmacodynamic components of the traditional Chinese medicine composition, including saikosaponin A, saikosaponin D, naringin, paeoniflorin, hesperidin, chicoric acid, chlorogenic acid, salvianolic acid B, zephyranthine, kaempferitrin, and codariusrhizome glycoside, amargin, hyperoside and rutin, thereby providing a reference for evaluating the intrinsic quality of the traditional Chinese medicine composition.

The invention discloses a method for synthesizing a metabolic engineering strain of chicoric acid from glucose and tartaric acid by saccharomyces cerevisiae. The method comprises the following steps: starting from a metabolic strain YS08 for producing chlorogenic acid; the method comprises the following steps: introducing hydroxyl cinnamoyl transferase AtHCT into saccharomyces cerevisiae; on the other hand, Echinacea hydroxyl cinnamoyl coenzyme A: tartaric acid hydroxyl cinnamoyl transferase EpHTT is introduced to convert caffeic acid coenzyme A and exogenous tartaric acid into caffeoyl tartaric acid, and the caffeoyl tartaric acid is a chlorogenic acid producing strain, so that two precursors for synthesizing chicoric acid are obtained; meanwhile, introducing echinacea purpurea chicoric acid synthase EpCAS to catalyze chlorogenic acid and caffeoyl tartaric acid to generate chicoric acid so as to obtain a chicoric acid producing strain CA01; the copy number of the three genes AtHCT-EpTT-EpCAS is increased to two, a metabolic strain CA02 is obtained, the yield of the chicoric acid can reach 12.5 mg / L, and the purpose that the saccharomyces cerevisiae uses glucose and tartaric acid to synthesize the chicoric acid is achieved for the first time.

The invention discloses a multi-index content determination method of hovenia acerba and rhizoma atractylodis bowel-relaxing granules, and belongs to the technical field of traditional Chinese medicine detection. The invention establishes a high performance liquid chromatography method for simultaneously determining nine components including caffeic acid, chicoric acid, naringin, naringin, hesperidin, neohesperidin, chrysophanol, aurantio-obtusin and atractylenolide I in a preparation, and the content of the components is calculated through a relative correction factor by taking caffeic acid as an internal reference by adopting a quantitative analysis of multi-components by single marker. According to the method, effective detection of the immature bitter orange medicinal material and the immature bitter orange base source is realized, and the immature bitter orange and the sweet orange base source of the traditional Chinese medicine immature bitter orange can be accurately distinguished, so that more comprehensive and accurate quality control of the traditional Chinese medicine is realized, and the stability and consistency of clinical efficacy of the traditional Chinese medicine are guaranteed.

This invention relates to the field of mosquito repellent composition technology, specifically to a plant-based mosquito repellent composition and its preparation method, comprising Sophora flavescens extract, eucalyptol, montmorillonite glycol, and natural Echinacea purpurea extract; the natural Echinacea purpurea extract is an aqueous extract with a chicoric acid content ≥4%. The plant-based mosquito repellent composition and preparation method of this invention, through functional optimization of single components and synergistic formulation of multiple components, fundamentally solves the core problems of existing plant-based mosquito repellent products, such as poor mosquito repellent effect, short effective duration, and lack of skin soothing function, while also taking into account the product's naturalness and safety. Because a combined low-temperature enzymatic hydrolysis-stepwise alcohol extraction-microfiltration purification process is used to prepare the Sophora flavescens extract, and natural menthol extract is added as a drug guide, the extraction rate and utilization efficiency of plant active ingredients are effectively improved, reducing the waste of plant resources. Furthermore, the preparation process is green and environmentally friendly, generating no harmful waste.

To provide a permanent wave pretreatment agent, a permanent wave solution, and a permanent wave method that offer excellent wave retention in permanent waves. [Solution] The permanent wave pretreatment agent contains one or more polyphenols selected from chicoric acid, gallic acid, tannic acid, tyriloside, and echinacoside; the permanent wave agent contains one or more polyphenols selected from chicoric acid, gallic acid, tyriloside, echinacoside, and quercetin, and a reducing agent; and the permanent wave method uses the above permanent wave pretreatment agent and / or permanent wave agent.

The invention discloses a preparation method and application of spirulina nanoemulsion, and belongs to the technical field of functional foods.The preparation method comprises the following steps that S1, a water phase is prepared, specifically, fresh spirulina mud is taken, washed and filtered, and a spirulina mud raw material is obtained; adding purified water of which the weight is 2-3 times that of the algae mud raw material into the algae mud raw material, and then carrying out superfine shearing treatment; centrifuging after the shearing treatment is finished, filtering and collecting filtrate to obtain a water phase; s2, preparing an oil phase: weighing egg yolk lecithin and coconut oil according to a mass ratio of 1: (0.5-0.8), and uniformly mixing and stirring the egg yolk lecithin and the coconut oil to obtain the oil phase; s3, preparing a compound emulsifier, wherein the compound emulsifier comprises the following components: tween-80, span-80, chicoric acid and 1, 2-propylene glycol in a mass ratio of (3-4): (5-7): (1-1.5): (4-5); and S4, emulsifying: mixing the water phase and the oil phase according to the mass ratio of 1: (0.5-1), adding a compound emulsifier which is 2-3 times of the mass of the mixture, and uniformly stirring to obtain the spirulina nanoemulsion. The spirulina nanoemulsion prepared by the preparation method disclosed by the invention has the effect of high stability.

The invention belongs to the technical field of Chinese patent medicine chemical component content detection, and particularly relates to a method for simultaneously detecting seven components of a cholagogic and analgesic tablet based on wavelength switching. According to the method, 70% methanol is adopted for ultrasonic extraction of a test sample, a C18 column is adopted as a stationary phase, methanol-0. 2% phosphoric acid is adopted as a mobile phase, gradient elution is combined with wavelength switching, good separation of (R, S)-epigoitrin, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin and ammonium glycyrrhizinate is achieved, and the method has good linearity, detection limit and quantitation limit. The method is simple to operate, can quantitatively determine seven active substances in the cholagogic and analgesic tablets at the same time, improves the quality control level and is beneficial to popularization.

The present application relates to the use of chicoric acid in the preparation of a medicament for preventing and / or treating myasthenia gravis. Specifically, the present application provides the use of chicoric acid and pharmaceutically acceptable salts, esters, solvates, polymorphs or prodrugs thereof in the preparation of a medicament for preventing and / or treating myasthenia gravis. The present application focuses on the use of the compound chicoric acid in correcting the core immune imbalance of myasthenia gravis, Th17 / Treg cell balance, intervening from the upstream of the disease, and is expected to achieve the fundamental treatment of myasthenia gravis and more persistent efficacy.

The invention discloses a pet health-care product capable of resisting inflammation and improving metabolism and a preparation method of the pet health-care product, and relates to the technical field of pet health-care products. Comprising fish oil micro-capsules, polyphenol micro-powder (containing a purple-leaf lettuce extract which is soluble in water and alcohol and insoluble in oil and needs to be adapted to a product system through an emulsification / embedding process), 10-20 parts of fish protein powder, 5-10 parts of dietary fibers, 1-3 parts of vitamin E, 2-5 parts of minerals and 1-2 parts of natural perfume. The preparation method of the fish oil micro-capsule is as follows. The polyphenol micro powder contains purple leaf lettuce extract (total polyphenol is not less than 4% and contains quercetin, chlorogenic acid, chicoric acid, anthocyanin and other polyphenols), tea polyphenol, curcumin and other components, so that free radicals can be eliminated to reduce oxidative damage and inhibit inflammatory pathways; amino acid small peptides provided by the fish protein powder can improve the intestinal absorption rate, enhance the barrier function of intestinal mucosa, and cooperate with the dietary fibers to regulate intestinal flora.

The invention provides an echinacea extract as well as a preparation method and application thereof. The extraction process of the echinacea purpurea extract is optimized through single factor and response surface experiments, the echinacea purpurea extract in which the ratio of the chicoric acid to the mono-caffeoyl tartaric acid can reach 34.1% is prepared, and the ratio of the chicoric acid can reach 20.8%. The prepared echinacea purpurea extract can remarkably improve the weight gain rate and the specific growth rate of largemouth bass, meanwhile, the survival rate and the disease resistance of the largemouth bass against aeromonas veronii infection can be improved, the risk of drug residues is avoided, and the echinacea purpurea extract is suitable for green aquaculture.

The invention relates to medical cold compress gel for improving gout and a preparation method of the medical cold compress gel. The medical cold compress gel comprises the following components in percentage by weight: (1) a cooling matrix material: 0.5-2% of carbomer, 5-15% of glycerol and 3-8% of propylene glycol; (2) natural anti-inflammatory components: 1-3% of a radix scutellariae extract and 0.5-1.5% of asiaticoside; (3) a uric acid inhibitor: 0.2%-1% of apigenin nano-liposome and 0.1%-0.5% of chicoric acid; (4) a transdermal penetration enhancer: 1-3% of azone and 0.5-2% of menthol; and the balance of deionized water. The invention has the following beneficial effects: 1, physical-chemical synergistic effect: red and swollen can be relieved by cold compress, and inflammatory factors such as IL-1beta, TNF-alpha and the like can be inhibited by the active components; 2, targeted inhibition of uric acid: apigenin nano-liposome enhances transdermal absorption and inhibits the activity of xanthine oxidase; 3, the safety is high: the systematic side effects of the traditional medicine are reduced by the natural components;

The application discloses a folate-chicoric acid liposome for treating ulcerative colitis and a preparation and application thereof, and relates to the technical field of veterinary drugs. The folate-chicoric acid liposome is composed of egg yolk lecithin, cholesterol, chicoric acid and folate (mass ratio of 10-14:2-5:1-2:1-2), has targeting property, has the effect of targeting macrophages, inhibits the polarization of macrophages to M1 type, can improve the clinical symptoms (weight loss, hematochezia and the like) of ulcerative colitis mice, increase the colon length, reduce the DAI score, the CMDI score and the colon index, relieve the pathological damage of the colon and relieve intestinal inflammation. The liposome has the effect of treating ulcerative colitis mice, and can provide an efficient drug for treating ulcerative colitis in the livestock breeding industry. Meanwhile, the liposome has the advantages of simple preparation method, good treatment effect, high specificity, safety and non-toxicity.

The application discloses a kind of two head tip standard decoction feature spectrum construction method, comprising the following steps: preparation reference solution, the reference solution includes control medicinal material reference solution and control reference solution, the control reference solution includes chicoric acid control product, bamboo joint spice element A control product and coffee acid control product in reference solution;Two head tip standard decoction is prepared for test solution;Take preset amount of reference solution and test solution, inject into liquid chromatograph, the liquid chromatograph is with octadecylsilane bonding silica gel as filler, with acetonitrile as mobile phase A, with phosphoric acid solution as mobile phase B and carries out gradient elution, establishes characteristic spectrum.The application can provide data basis for two head tip standard decoction, medicinal material quality control, effectively guarantee the stability and controllability of two head tip medicinal material, standard decoction product quality.

The invention discloses application of chicoric acid in preparation of a medicine for improving hematopoietic injury caused by radiation, and belongs to the technical field of biological medicine. In order to solve the problem that an effective intervention means for hematopoietic system injury caused by ionizing radiation is lacked in the prior art, the invention provides application of chicoric acid in preparation of drugs for improving hematopoietic injury caused by radiation, and shows a dual protection mechanism of chicoric acid acting on a hematopoietic differentiation key regulation node. The hematopoietic function is synergistically improved by regulating EPO and other hematopoietic factors and progenitor cell populations, meanwhile, the anti-oxidation effect is achieved, radiation damage is relieved, definite active ingredients and action targets are provided for preparing the medicine for preventing and treating hematopoietic damage caused by radiation, and the application value of clinical transformation is achieved.

The invention belongs to the technical field of plant extraction, and particularly relates to a dandelion extract as well as a preparation method and application thereof. The method comprises the following steps: crushing dandelion to obtain raw material particles, then mixing the raw material particles with a vitamin solution, heating, stirring, filtering and separating filter residues and filtrate, enabling the filtrate to pass through a membrane, taking trapped fluid, concentrating, contacting with ion exchange resin, eluting to obtain eluent, and removing a solvent to obtain the dandelion extract. According to the preparation method of the dandelion extract, after the raw materials are subjected to superfine grinding, low-temperature stirring and acid-alcohol short-time extraction are adopted, the chicoric acid component can be effectively reserved, and effective components are prevented from being damaged in the extraction process. In addition, the grain-saving feed is prepared by comprehensively utilizing extracts and utilizing extraction residues, chicoric acid, molasses, sorghum stalks, siraitia grosvenorii residues, stevia rebaudiana and chrysanthemum residues and salt, and the feed can be used for raising cattle, pigs and poultry.

The invention discloses a feed additive for relieving liver glycogen accumulation of micropterus salmoides and application of the feed additive. The feed additive comprises a compound plant extract and an amino acid mixture, the part ratio of the composite plant extract to the amino acid mixture is (22-27): (50-60); the composite plant extract is prepared from curcumin, resveratrol, chicoric acid, berberine, quercetin, licoflavone, astragalus polysaccharide, zanthoxylin and danshinone; the amino acid mixture is prepared from L-arginine, L-leucine, L-lysine and L-alanine. Preferably, the feed additive also comprises 18-22 parts of coated short-chain fatty acid salt. The components of the feed additive can jointly activate insulin signal channels of the micropterus salmoides, promote glycolysis, inhibit gluconeogenesis, relieve insulin resistance induced by high glucose and reduce liver glycogen accumulation of the micropterus salmoides.

The invention relates to the technical field of medicines, in particular to a chicoric acid self-microemulsion, a chicoric acid self-microemulsion preparation and a preparation method of the chicoric acid self-microemulsion preparation. According to the chicoric acid self-microemulsion under the optimal formula, the average drug loading capacity is 69.50 + / -0.31 mg / g, the average particle size is 15.01 + / -0.25 nm, and the average PDI is 0.251 + / -0.01. The chicoric acid self-microemulsion has good stability. The chicoric acid self-microemulsion pellet prepared from the chicoric acid self-microemulsion pellet also has good stability.

The present application relates to the technical field of biotechnology, and particularly relates to application of chicoric acid (especially LCA) in preparation of a medicine for preventing, alleviating and / or treating adverse pregnancy outcomes caused by over-activation of IFN-I.

This invention relates to the field of natural product extracts and mitochondrial quality control regulation technology, and provides a mitochondrial quality control regulation extract composition derived from red lettuce and its uses. The composition contains active ingredients such as chlorogenic acid, chicoric acid, quercetin glycosides, and anthocyanins, and possesses a stable HPLC fingerprint. Experimental results show that the composition can significantly restore ATP levels in cells damaged by oxidative stress, improve mitochondrial membrane potential and respiratory function, activate AMPK-related pathways, inhibit downstream mTORC1 signaling, enhance mitophagy, and promote mitochondrial regeneration, thereby achieving synergistic regulation of the mitochondrial quality control system. The composition has the advantages of well-defined composition, batch stability, and overall superior effects compared to artificial mixtures, making it suitable for preparing products that regulate mitochondrial function.

The application belongs to the technical field of Chinese medicine chemical component content detection, and particularly relates to a method for simultaneously detecting seven components in Lidan Zhitong tablets based on wavelength switching. The method adopts 70% methanol ultrasonic extraction of the test product, adopts a C18 column as a stationary phase, methanol-0.2% phosphoric acid as a mobile phase, realizes good separation of (R,S)-adonirubin, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin and ammonium glycyrrhizinate through gradient elution combined with wavelength switching, and has good linearity, a detection limit and a quantitative limit. The method is simple in operation, can simultaneously quantitatively detect seven active substances in the Lidan Zhitong tablets, improves the quality control level and is beneficial to popularization.

The invention discloses a fingerprint detection method for a Puyuan stomach harmonizing capsule, and belongs to the field of traditional Chinese medicine detection. Comprising the following steps: performing high performance liquid chromatography detection on a system applicability solution, a reference substance solution, a medicinal material solution and a test solution to obtain chromatograms; chemical components of chromatographic peaks in the fingerprint spectrum are determined according to the chromatogram, the chemical components are caffeic acid serving as a first peak, tetrahydropalmatine serving as a second peak, chicoric acid serving as a third peak, ammonium glycyrrhizinate serving as a sixth peak, alpha-cyperone serving as an eighth peak and 11-carbonyl-beta-acetyl masticinic acid serving as a tenth peak, the second peak and the fourth peak are derived from rhizoma corydalis, the eighth peak is derived from rhizoma cyperi, the seventh peak, the ninth peak and the tenth peak are derived from frankincense, and the seventh peak, the ninth peak and the tenth peak are derived from beta-acetyl masticinic acid. The No.1 peak, the No.3 peak and the No.5 peak are derived from dandelion, and the No.6 peak is derived from liquorice. The method is applied to the detection of the Puyuan stomach harmonizing capsule, and solves the problems of complicated operation and single quality control index of rhizoma corydalis, rhizoma cyperi and vinegar frankincense in the existing detection method.

The invention relates to the technical field of deep processing of natural products, in particular to a fermentation preparation method of an active dandelion extract with functions of removing stasis and resisting inflammation, which comprises the following steps: crushing dried whole dandelion, and carrying out synergistic enzymolysis by using a compound enzyme consisting of cellulase, pectinase and beta-glucanase; then carrying out primary anaerobic fermentation of lactobacillus plantarum and secondary aerobic fermentation of issatchenkia orientalis in sequence; and finally, centrifuging, filtering, concentrating and spray-drying to obtain the high-activity dandelion fermentation extract. Through a process chain of ternary composite enzymolysis and step-by-step fermentation, an anaerobic-aerobic alternating micro-ecological system is constructed, and synergistic enrichment and functional quantification of double active components are realized. Specifically, in the final fermentation extract, the content of chicoric acid is greater than or equal to 8.5%, and the content of small molecular glycopeptide is greater than or equal to 25%, which are far higher than those of the traditional aqueous extract.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing chlorogenic acid and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, a hydroxylation module and a condensation module are constructed in the Yarrowia lipolytica, heterologous synthesis of CGA is realized, supply of NADPH is promoted through optimization of an endogenous pentose phosphate pathway, oxygen intake is increased and a shikimic acid pathway is enhanced by using hemoglobin VHb of heterologous Vitreoscilla, and the yield of chlorogenic acid is further increased. The yield of chlorogenic acid of the constructed engineering bacterium can reach 13.13 g / L, a foundation is laid for subsequent biosynthesis of compounds such as chicoric acid, and the engineering bacterium has potential value and significance for development of synthetic biology.

The invention discloses a preparation method of chicken small intestine epithelial cell apoptosis vesicles. The preparation method comprises the following steps: culturing chicken small intestine epithelial cells; inducing cell apoptosis; and preparing the apoptosis vesicles. The invention also discloses the chicken small intestine epithelial cell apoptosis vesicle prepared by the method and application thereof. The chicken small intestine epithelial cell apoptosis vesicle can load chicoric acid, and has the effects of resisting inflammation, enhancing cell oxidation resistance and promoting chicken small intestine epithelial cell proliferation and cell migration.