Method for simultaneously detecting seven components of cholagogic and analgesic tablets based on wavelength switching

Abstract

The invention belongs to the technical field of Chinese patent medicine chemical component content detection, and particularly relates to a method for simultaneously detecting seven components of a cholagogic and analgesic tablet based on wavelength switching. According to the method, 70% methanol is adopted for ultrasonic extraction of a test sample, a C18 column is adopted as a stationary phase, methanol-0. 2% phosphoric acid is adopted as a mobile phase, gradient elution is combined with wavelength switching, good separation of (R, S)-epigoitrin, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin and ammonium glycyrrhizinate is achieved, and the method has good linearity, detection limit and quantitation limit. The method is simple to operate, can quantitatively determine seven active substances in the cholagogic and analgesic tablets at the same time, improves the quality control level and is beneficial to popularization.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of Chinese medicine chemical component content detection, and particularly relates to a method for simultaneously detecting seven components in Lidan Zhitong tablets based on wavelength switching. BACKGROUND

[0002] Lidan Zhitong tablets are compound preparations prepared from 12 Chinese medicines, including Radix Bupleuri, Radix Paeoniae Rubra, Fructus Aurantii, Radix Glycyrrhizae, Herba Artemisiae Scopariae, Rhizoma Corydalis, Radix Atractylodis, Fructus Xanthii, Herba Lycopodii, Radix Isatidis, Herba Taraxaci and Rhizoma Curcumae, and have the effects of clearing heat and promoting bile secretion and regulating qi and relieving pain. Lidan Zhitong tablets are used for treating hypochondriac pain and jaundice (such as acute and chronic hepatitis and cholecystitis) caused by damp-heat of liver and gallbladder. Modern pharmacological researches conducted in China have confirmed that Lidan Zhitong tablets have the related pharmacological activities and effects of protecting liver, anti-inflammation, analgesia and regulating cholesterol (Zhang Liqun, et al. Pharmacodynamic study of Lidan Zhitong tablets [J]. Yunnan Journal of Chinese Medicine and Pharmacy, 2012, 33 (8): 55-58).

[0003] The original quality standard of Lidan Zhitong tablets is recorded in the Eleventh Volume of Traditional Chinese Medicine Prescription Preparations of the Ministry of Health Drug Standards, and has simple physicochemical identification items but no content determination item, so that effective quality control and monitoring cannot be performed. Although quantitative detection researches on Lidan Zhitong tablets have been carried out in recent years, due to the unknown nature of the chemical components (especially the active components) of the compound preparations and the mutual interference of the complex components, only a single component liquid chromatography analysis method for paeoniflorin in Lidan Zhitong tablets has been established. And due to the interference of a large number of unknown components, a complex pretreatment program including a complex purification step of a multi-solvent system is required (Fu Yue, et al. Research on quality standard of Lidan Zhitong tablets [J]. Chinese Patent Medicine, 2005, 27 (4): 411-413).

[0004] Therefore, there is an urgent need in the art to establish a multi-component simultaneous quality control detection method for Lidan Zhitong tablets to improve the quality control and monitoring level. SUMMARY

[0005] In view of the above problems, the present application found in the previous research that there are seven components in the finished preparation of Lidan Zhitong tablets, including (R, S)-adipocytokinins, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin and ammonium glycyrrhizinate. Among them, the published research results show that (R, S)-adipocytokinins have anti-inflammatory and antiviral activities; paeoniflorin has analgesic effect; chicoric acid has anti-inflammatory, antiviral and inhibitory effect on secondary liver damage caused by inflammation; neohesperidin has antioxidant and blood lipid regulating activities; chlorogenic acid, naringin and ammonium glycyrrhizinate have anti-inflammatory, liver protection or inhibitory effect on liver damage.

[0006] In view of the above ingredients and the known pharmacological activity and efficacy of the Dantie Tongpi tablet, it is suitable as a quality control ingredient of the Dantie Tongpi tablet. The purpose of the present application is to provide a method for simultaneously detecting seven components of the Dantie Tongpi tablet based on wavelength switching, so as to realize simultaneous quantitative detection of the seven components of the Dantie Tongpi tablet, including (R, S)-adipocire, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate. The technical solution of the present application is as follows: A method for simultaneously detecting seven components of the Dantie Tongpi tablet based on wavelength switching, the method is liquid chromatography, the liquid chromatography comprises the following steps: Preparation of test sample solution: take the test sample, remove the sugar coating, grind into powder, add 70% methanol, ultrasonic extraction, filter to prepare the test sample solution; Preparation of control sample solution: take (R, S)-adipocire, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate control sample, add 70% methanol to prepare the control sample solution; Sample injection and detection: respectively take the control sample solution and the test sample solution and inject into the liquid chromatograph for detection, draw the standard curve, and calculate the content of the seven components; the chromatographic column of the liquid chromatograph is a C18 column, and the column length, inner diameter, and particle size of the chromatographic column are 250 mm, 4.6 mm, and 5 μm, respectively; methanol is used as the mobile phase A, and 0.2% phosphoric acid is used as the mobile phase B, the flow rate is 1.0 mL / min, gradient elution is carried out, and the flow phase gradient elution program is as follows:

[0007] The above gradient elution program can also be expressed as: 0~6min, 10% methanol; 6~11min, 10%→20% methanol; 11~20min, 20%→24% methanol; 20~30min, 24%→30% methanol; 30~53min, 30%→44% methanol; 53~55min, 44%→60% methanol; 55~72min, 60%→80% methanol; 72~73min, 80%→10% methanol; 73~80min, 10% methanol.

[0008] The detection wavelength of the liquid chromatography is as follows:

[0009] In combination with the flow phase elution program, the above wavelength switching program can also be expressed as: 0~13min, 245nm; 13~23min, 327nm; 23~30min, 230nm; 30~40min, 327nm; 40~60min, 283nm; 60~80min, 237nm. In other words, in the wavelength switching program, the corresponding time points in the time column are the time points of wavelength switching, and the time points not listed do not have wavelength switching.

[0010] Preferably, the ultrasonic power is 300 W when the test sample solution is prepared, and the ultrasonic extraction time is 45 min to 80 min.

[0011] Preferably, the ultrasonic frequency is 50 kHz when the test sample solution is prepared.

[0012] Preferably, the concentration of the test sample solution is 0.3 g to 0.8 g of the test sample per 25 mL of the test sample solution. Further preferably, the concentration of the test sample solution is 0.4 g to 0.6 g of the test sample per 25 mL of the test sample solution.

[0013] Preferably, the control sample solution contains (R, S)-ademetionine, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate at 2 μg, 20 μg, 80 μg, 10 μg, 100 μg, 90 μg, and 20 μg per 1 mL, respectively.

[0014] Preferably, the column temperature of the liquid chromatography is 30°C.

[0015] Preferably, the injection volume of the liquid chromatography is 10 µL.

[0016] Preferably, the chromatograph used in the liquid chromatography is an Agilent 1260 II high-performance liquid chromatograph.

[0017] Preferably, the C18 column used in the liquid chromatography is a Shimadzu Shim-pack GIST C18 column.

[0018] Advantages

[0019] (1) Compared with the prior art, the detection method of the present application greatly simplifies the test sample extraction method, and the chromatographic separation effect of each active substance is good and suitable for quantitative analysis of multiple components.

[0020] (2) Compared with the prior art, the detection method of the present application can simultaneously quantitatively detect up to 7 active substances in the Dicang Zhitong tablets, thereby improving the quality control level of the Dicang Zhitong tablets, facilitating quality control and drug supervision. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 is a control sample chromatogram of Example 1; Figure 2 is a test sample chromatogram of Example 1; Figure 3 is a test sample chromatogram of Example 2 with methanol as the extraction solvent; Figure 4 is a test sample chromatogram of Example 2 with ethanol as the extraction solvent; Figure 5 The sample chromatogram of Example 2 with 50% methanol as the extraction solvent; Figure 6 The sample chromatogram of Example 2 with 70% methanol as the extraction solvent; Figure 7 The sample chromatogram of Example 2 with water as the extraction solvent; Figure 8 The sample chromatogram of Example 3 with methanol-water as the mobile phase for gradient elution; Figure 9 The sample chromatogram of Example 3 with methanol-1% glacial acetic acid as the mobile phase for gradient elution; Figure 10 The sample chromatogram of Example 3 with acetonitrile-0.2% phosphoric acid as the mobile phase for gradient elution; Figure 11 The sample chromatogram of Example 3 with methanol-0.2% phosphoric acid as the mobile phase for gradient elution. DETAILED DESCRIPTION

[0022] The schemes and effects of the present application will be further illustrated below in conjunction with specific examples. It should be understood that the specific examples are only used to explain the present application and not used to limit the protection scope of the present application.

[0023] The instruments, reagents, sample sources and models used in the following examples and exploratory examples are as follows: Instruments: Agilent 1260 II high performance liquid chromatograph (Agilent Technology Co., Ltd.); MS105DU electronic balance (Mettler Toledo Technology (China) Co., Ltd.); XPR10 electronic balance (Mettler Toledo Technology (China) Co., Ltd.); Model 9960D ultrasonic instrument (CBL Photoelectron Technology Co. Ltd.).

[0024] Reagents: (R, S)-adonirubin (batch number: 111753-202408, content 100.0%), chlorogenic acid (batch number: 110753-202119, content 96.3%), paeoniflorin (batch number: 110736-202548, content 98.9%), chicoric acid (batch number: 111752-202105, content 98.3%), naringin (batch number: 110722-202417, content 96.1%), neohesperidin (batch number: 111857-202305, content 99.6%), ammonium glycyrrhizinate (batch number: 110731-202423, content 93.2%) were all purchased from China Institute for Drug Control, and experimental water was ultrapure water, methanol and phosphoric acid were chromatographically pure, and other reagents were analytically pure.

[0025] Sample: Lidan Zhongtong Tablets, Jilin Junhong Pharmaceutical Co., Ltd.

[0026] Example 1 Method for simultaneous detection of 7 components in Lidan Zhongtong Tablets by HPLC wavelength switching method This example demonstrates a chromatographic method (sample preparation and detection conditions) for simultaneous detection of 7 components in Lidan Zhongtong Tablets based on HPLC wavelength switching method. The sample preparation and detection conditions of this method are as follows: (1) Preparation of test solution: Take the test sample, remove the sugar coating, grind into powder, pass through a No. 5 sieve, take about 0.5 g, accurately weigh, place in a conical flask, accurately add 25 mL of 70% methanol, weigh, ultrasonic treat (power 300 W, frequency 50 kHz) for 45 min, cool, re-weigh, add 70% methanol to make up for the weight loss, shake well, filter, and take the filtrate, which is obtained.

[0027] (2) Preparation of control solution (i.e. mixed control solution): Take the appropriate amount of control substance, accurately weigh, add 70% methanol to make a solution containing (R, S)-ademetionine, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate at 2 μg, 20 μg, 80 μg, 10 μg, 100 μg, 90 μg, and 20 μg per 1 mL, respectively, which is obtained.

[0028] (3) Chromatographic conditions: The chromatographic column is Shim-pack GIST C18 (250 x 4.6 mm, 5 µm) from Shimadzu; methanol is used as mobile phase A, and 0.2% phosphoric acid is used as mobile phase B, eluted by gradient according to Table 1, with a flow rate of 1.0 mL / min, column temperature of 30°C, injection volume of 10 µL, and detection wavelength switching program as shown in Table 2. The chromatograms of the control and test samples are shown in Figure 1 , Figure 2 .

[0029] Table 1 Gradient elution program

[0030] Table 2 Detection wavelength switching program

[0031] As can be seen from Figure 1 , Figure 2 , under this method, each target substance is well separated and suitable for qualitative and quantitative detection.

[0032] Example 2 Methodology investigation and sample determination of simultaneous detection of 7 components in Lidan Zhongtong Tablets by HPLC wavelength switching method This example demonstrates the methodology investigation research content for the method described in Example 1.

[0033] 1. Investigation and results of extraction solvent Take the test product, remove the sugar coating, grind into powder, pass through a No. 5 sieve, take about 0.50 g, accurately weigh 5 portions of about 0.50 g each, add 70% methanol 25 mL into conical flasks, and accurately weigh. Ultrasonic extraction (power 300 W, frequency 50 kHz) for 30 min, 45 min, and 60 min for the samples in the conical flasks, respectively; heating reflux extraction for 30 min and 45 min for the samples in the flasks, respectively; cool, reweigh, make up the weight loss with 70% methanol, shake well, filter through a 0.45 μm filter membrane, take 10 μL of the filtrate and inject it into the liquid chromatograph, and record the chromatogram. The detection results are shown in Table 4. The results show that the ultrasonic extraction and heating reflux extraction have similar effects, and the component content basically does not increase after 45 min of ultrasonic extraction. Therefore, the extraction method is ultrasonic extraction for 45 min. Figures 3-7 The results show that the extraction of each component is sufficient with 70% methanol as the extraction solvent, and the peak type is good, and the separation degree meets the requirements, so 70% methanol is selected as the extraction solvent.

[0034] Table 3 Investigation results of different extraction solvents (peak area value)

[0035] 2. Investigation of extraction method and extraction time and results Take the test product, remove the sugar coating, grind into powder, pass through a No. 5 sieve, take about 0.50 g, accurately weigh 5 portions of about 0.50 g each. Add 70% methanol 25 mL into conical flasks, and accurately weigh. Ultrasonic extraction (power 300 W, frequency 50 kHz) for 30 min, 45 min, and 60 min for the samples in the conical flasks, respectively; heating reflux extraction for 30 min and 45 min for the samples in the flasks, respectively; cool, reweigh, make up the weight loss with 70% methanol, shake well, filter through a 0.45 μm filter membrane, take 10 μL of the filtrate and inject it into the liquid chromatograph, and record the chromatogram. The detection results are shown in Table 4. The results show that the ultrasonic extraction and heating reflux extraction have similar effects, and the component content basically does not increase after 45 min of ultrasonic extraction. Therefore, the extraction method is ultrasonic extraction for 45 min.

[0036] Table 4 Investigation results of extraction method and extraction time (peak area value)

[0037] 3. Investigation results of linearity and range Take the control sample solution prepared according to the method of Example 1, dilute it into a series of concentrations, and inject it into the liquid chromatograph according to the chromatographic conditions of Example 1. Regress the control sample concentration as the abscissa (X) and the peak area as the ordinate (Y) to draw a standard curve. The results are shown in Table 5. The results show that each component has a good linear relationship within its respective range.

[0038] Table 5 Linear relationship and linear range of each component

[0039] 4. Precision test and results Take the control solution prepared according to the method of Example 1, continuously sample 6 times, and determine according to the chromatographic conditions of Example 1. The retention time RSD (n = 6) of (R, S)-jodopyronet, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate is 0.2%~1.3%; the peak area RSD (n = 6) is 0.3%~1.7% (results are shown in Table 6, Table 7). The results show that the instrument precision is good.

[0040] Table 6 Retention time RSD

[0041] Table 7 Peak area RSD

[0042] 5. Repetitive test and results Prepare 6 portions of sample solution according to the method of Example 1, inject into the liquid chromatograph, and determine according to the chromatographic conditions of Example 1. Calculate the peak area RSD (n = 6) of (R, S)-jodopyronet, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate, and the results are 0.5%~1.9% (results are shown in Table 8). The results show that the method has good repeatability.

[0043] Table 8 Repetitive test results

[0044] 6. Quantitative limit and detection limit Take the control solution prepared according to the method of Example 1, dilute step by step, determine according to the chromatographic conditions of Example 1, and record the chromatogram. When the signal-to-noise ratio S / N is 3, the corresponding concentration is the detection limit, and when the S / N is 10, the corresponding concentration is the quantitative limit. The detection limit of (R, S)-jodopyronet, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate is 0.011 µg / mL, 0.023 µg / mL, 0.109 µg / mL, 0.024 µg / mL, 0.054 µg / mL, 0.024 µg / mL, and 0.148 µg / mL, respectively; the quantitative limit is 0.032 µg / mL, 0.068 µg / mL, 0.381 µg / mL, 0.118 µg / mL, 0.180 µg / mL, 0.094 µg / mL, and 0.262 µg / mL, respectively.

[0045] 7. Sample recovery test and results Take 6 conical flasks, respectively, add the appropriate amount of control solution prepared according to the method of Example 1, and dry the solvent in a water bath. Then take about 0.5 g of the product powder (test sample powder, prepared according to the method of Example 1) and accurately weigh it into each of the conical flasks. Accurately add 25 mL of 70% methanol, weigh, ultrasonic treat (power 300 W, frequency 50 kHz) for 45 min, cool, re-weigh, make up the weight loss with 70% methanol, shake well, filter, take 10 μL of the filtrate and inject it into the liquid chromatograph, and determine the peak area of each component according to the chromatographic conditions of Example 1. The average sample addition recovery rates of (R, S)-adonirubin, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate are 95.2%, 98.1%, 98.2%, 97.7%, 97.3%, 99.2%, and 97.9%, respectively, and the RSDs are 1.58%, 1.24%, 1.50%, 1.32%, 1.11%, 1.19%, and 1.77%, respectively (see Table 9), indicating that the method has good accuracy.

[0046] Table 9 Sample addition recovery rate experiment

[0047]

[0048] 8. Sample detection and results Take 3 batches of samples, prepare the test sample solution according to the method of Example 1, and determine the sample according to the chromatographic conditions of Example 1. The results are shown in Table 10. The contents of each target substance in the 3 batches of samples fluctuate slightly, but the difference is small.

[0049] Table 10 Sample content determination results

[0050] Example 3 Method for simultaneous detection of 7 components in Dianzhitongpi tablets by HPLC wavelength switching The following is a display of some of the exploration examples in the process of establishing the detection method of the present application. Each exploration example shows the test sample chromatogram under the optimal gradient elution program, wavelength switching program, and flow rate of the mobile phase.

[0051] The test sample preparation method of the exploration example is the same as that of Example 1, the flow rate of the mobile phase is 1.0 mL / min, and the column temperature is 30°C. The detection instrument is an Agilent 1260 II high performance liquid chromatograph (Agilent Technologies), the chromatographic column is Shim-pack GIST C18 (250 x 4.6 mm, 5 µm), and the injection volume is 10 µL. The results are shown in Figures 8-11 , respectively.

[0052] Exploration Example 1: Figure 8 The mobile phase used was methanol-water, methanol as mobile phase A, water as mobile phase B, gradient elution, the mobile phase gradient elution program is shown in Table 11: Table 11 Gradient elution program of exploration example 1

[0053] Figure 8 And the corresponding wavelength switching program of Table 11 is: 0~13min 245nm; 13~23min 327nm; 23~30min 230nm; 30~40min 327nm; 40~60min 283nm; 60~80min 237nm. From Figure 8 It can be seen that under this detection method, chlorogenic acid, chicoric acid and ammonium glycyrrhizinate do not peak.

[0054] Exploration Example 2: Figure 9 The mobile phase used was methanol-1% glacial acetic acid, methanol as mobile phase A, 1% glacial acetic acid as mobile phase B, gradient elution, the mobile phase gradient elution program is shown in Table 12.

[0055] Table 12 Gradient elution program of exploration example 2

[0056] Figure 9 And the corresponding wavelength switching program of Table 12 is: 0~13min 245nm; 13~23min 327nm; 23~30min 230nm; 30~40min 327nm; 40~60min 283nm; 60~80min 237nm. From Figure 9 It can be seen that under this detection method, paeoniflorin and chicoric acid peaks cannot be separated, and ammonium glycyrrhizinate does not peak.

[0057] Exploration Example 3: Figure 10 The mobile phase used was acetonitrile-0.2% phosphoric acid, acetonitrile as mobile phase A, 0.2% phosphoric acid as mobile phase B, gradient elution, the mobile phase gradient elution program is shown in Table 13.

[0058] Table 13 Gradient elution program of exploration example 3

[0059] Figure 10 And the corresponding wavelength switching program of Table 13 is 0~16min 245nm; 16~23min 327nm; 23~35min 230nm; 35~45min 327nm; 45~60min 283nm; 60~80min 237nm. From Figure 10It can be seen that under the detection method, the separation degree of paeoniflorin, chicoric acid and adjacent unknown peaks does not meet the requirements, and it is difficult to be used for quantitative analysis.

[0060] Exploration example 4: Figure 11 The mobile phase used is methanol-0.2% phosphoric acid, methanol as mobile phase A, 0.2% phosphoric acid as mobile phase B, gradient elution, the mobile phase gradient elution program is shown in Table 14.

[0061] Table 14 Gradient elution program of exploration example 4

[0062] Figure 11 And the corresponding wavelength switching program of Table 14 is 0~13min 245nm; 13~21min 327nm;21~28min 230nm;30 28~36min 327nm;36~55min 283nm;55~70min 237nm. From Figure 11 It can be seen that under the detection method, the separation degree of paeoniflorin, chicoric acid and adjacent unknown peaks does not meet the requirements, and it is difficult to be used for quantitative analysis.

[0063] The detection method of the present application is a multi-component simultaneous quantitative analysis method of Lidan Zhitong tablets obtained by a large amount of exploration analysis. It can be known from the comparison of the above examples and exploration examples that, compared with other detection methods, the detection method of the present application realizes the simultaneous quantitative determination of a plurality of active substances in Lidan Zhitong tablets, which not only improves the quality control and monitoring level, but also simplifies the operation of quality detection, and is conducive to popularization and promotion.

Claims

1. A method for simultaneous detection of 7 components in Lidan Zhitong tablets based on wavelength switching, characterized in that, The method is a liquid chromatography method comprising the following steps: Preparation of the test sample solution: take the test sample, remove the sugar coating, grind into powder, add 70% methanol, ultrasonic extraction, filter, and prepare the test sample solution; the concentration of the test sample solution is 0.3g-0.8g of the test sample per 25mL of the test sample solution; Preparation of the control sample solution: take the (R,S)-adjuvant, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate control samples, add 70% methanol, and prepare the control sample solution; the control sample solution contains 2ug of (R,S)-adjuvant, 20ug of chlorogenic acid, 80ug of paeoniflorin, 10ug of chicoric acid, 100ug of naringin, 90ug of neohesperidin, and 20ug of ammonium glycyrrhizinate per 1mL; Injection detection: take the control sample solution and the test sample solution respectively, inject them into the liquid chromatograph for detection, draw the standard curve, and calculate the content of the seven components; the chromatographic column of the liquid chromatograph is a C18 column, and the column length, inner diameter, and particle size of the chromatographic column are 250mm, 4.6mm, and 5um respectively; methanol is used as the mobile phase A, 0.2% phosphoric acid is used as the mobile phase B, the flow rate is 1.0mL / min, gradient elution is performed, and the flow phase gradient elution program is as follows: ； The detection wavelength switching program of the liquid chromatography method is as follows: 。 2. The method for simultaneous detection of 7 components in Lidan Zhitong tablets according to claim 1, characterized in that, When preparing the test sample solution, the ultrasonic power is 300W, and the ultrasonic extraction time is 45min-80min.

3. The method for simultaneous detection of 7 components in Lidan Zhitong tablets according to claim 2, characterized in that, When preparing the test sample solution, the ultrasonic frequency is 50kHz.

4. The method according to claim 1, wherein the method is characterized in that, The concentration of the test sample solution is 0.4g-0.6g of the test sample per 25mL of the test sample solution.

5. The method according to claim 1, wherein the method is characterized in that, The column temperature of the liquid chromatography method is 30℃.

6. The method according to claim 1, wherein the 7 components of Lidan Zhitong tablets are simultaneously detected by wavelength switching. The injection amount of the liquid chromatography method is 10µL.

7. The method according to claim 1, wherein the method is characterized in that, The chromatograph used in the liquid chromatography method is an Agilent 1260Ⅱ high-performance liquid chromatograph.

8. The method according to claim 1, wherein the method is based on wavelength switching for simultaneous detection of 7 components of Lidan Zhitong tablets. The C18 column used in the liquid chromatography method is a Shimadzu Shim-pack GIST C18 column.

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