The invention discloses an anti-radiation Chinese medicine preparation and a preparation method thereof. The preparation is a capsule which is prepared by mixing extract powder of a Chinese medicine composition and microcrystalline cellulose with the weight ratio of 2:1, wherein the Chinese medicine composition consists of ingredients according to parts by weight: 160-200 parts of astragalus mongholicus, 110-130 parts of fructus psoraleae, 80-100 parts of angelica sinensis, 160-200 parts of herba epimedii, 135-165 parts of madder root, 110-130 parts of fructus ligustri lucidi, 110-130 parts of radix paeoniae alba, 110-130 parts of radix rehmanniae preparata, 80-100 parts of ligusticum wallichii, 215-265 parts of rhizoma dioscoreae and 35-45 parts of radix glycyrrhizae preparata, and the extract powder is dry powder of ethanol solution extract of the Chinese medicine composition. The anti-radiation Chinese medicine preparation is precise in anti-radiation treatment effect, low in toxicside effects, simple in preparation method, low in cost, convenient to carry, easy to store, convenient to take and accurate in dosage, thus having good development and application prospect in the prevention and treatment of radiation injury.