The invention discloses a device for preparing a radioactive sample. The device comprises a radiation shielding body and a moving carrier, wherein the radiation shielding body comprises a box body; one end of the box body is an open end, and the open end is connected with a side opening door; the top of the box body is provided with an opening group, the opening group comprises at least one opening, and the openings communicate with internal and external spaces of the box body; at least part of the box body and the side opening door is made of a radiation shielding material; and the moving carrier comprises a placing part, the placing part is provided with a clamping groove group, the clamping groove group comprises at least one clamping groove, and the clamping grooves are used for placing an experiment bottle. According to the device, the dosage level of the sample in an experiment process is significantly reduced to a few micro-sieverts, so that experiment operations of dissolutionof the radioactive sample, pH value adjustment and determination, filtration and separation of the sample and the like can be completed outside a glove box, an operator can complete the fine operations more efficiently, experiment time is shortened, time of exposure in a radiation environment is shortened, and the health and safety of experimenters are guaranteed.