1688 results about "Proteases" patented technology

The invention relates to a stable silk fibroin liquid condensation body, gel and a preparation method and application thereof, and belongs to the technical field of silk fibroin. The preparation method comprises the following steps: S1, dissolving silk fibroin in a lithium bromide solution, and then adding trypsin for incubation to obtain an enzyme-digested silk fibroin solution; and S2, performing low-temperature programmed centrifugal treatment including an induction stage, a nucleation stage and a liquid-liquid phase separation stage on the enzyme-digested silk fibroin solution, and cleaning to obtain the stable silk fibroin liquid-state aggregate, the method can reduce aggregate liquid drop aggregation and improve the particle size uniformity of the liquid drops.

Described are RNAi agents, compositions that include RNAi agents, and methods for dual inhibition of an Apolipoprotein C-III (APOC3) and Proprotein Convertase Subtilisin Kexin 9 (PCSK9) gene. The APOC3-PCSK9 RNAi agents disclosed herein inhibit the expression of an APOC3 and a PCSK9 gene. Pharmaceutical compositions that include one or more APOC3-PCSK9 RNAi agents, optionally with one or more additional therapeutics, are also described. Delivery of the described TSLP RNAi agents to hepatic cells, in vivo, provides for inhibition of APOC3 and / or PCSK9 gene expression, which can provide a therapeutic benefit to subjects, including human subjects, for the treatment of various diseases including hypertriglyceridemia and hypercholesterolemia.

The invention discloses staphylococcus NHY-25 capable of producing enzyme and aroma, which is preserved in Guangdong Microbial Culture Collection Center on May 26, 2025, and the preservation number is GDMMC NO: 66396. When the strain is applied to yeast strengthening, the enzyme activity in high-temperature yeast can be improved, for example, the activity of protease, saccharifying power, fermenting power and liquefying power of yeast can be improved. Through detection of volatile compounds in the strengthened Daqu and the unstrengthened Daqu, it is found that the strain enables the variety and content of the volatile compounds in the strengthened Daqu to be improved. The strain enables the sauce flavor of high-temperature yeast to be more prominent, can be applied to yeast strengthening, and provides a new thought for improving the quality of yeast.

The invention discloses a tightening and whitening composition containing EGCG (epigallocatechin gallate) as well as a preparation method and application of the tightening and whitening composition, and relates to the technical field of cosmetics. The invention provides a tightening and whitening composition containing EGCG (epigallocatechin gallate). The tightening and whitening composition is prepared from the following components in parts by weight: 0.1 to 2 parts of an epigallocatechin gallate nano composition, 0.05 to 0.5 part of a nucleic acid tetrahedron and 0.5 to 5 parts of glassine. When the EGCG nano composition, the nucleic acid tetrahedron and the glassine are cooperatively used, the stability of a formula can be improved, meanwhile, the EGCG can be accurately released to an epidermal layer due to the enzyme-responsive degradation characteristic (such as matrix metalloproteinase MMP-2 triggered depolymerization) of the nucleic acid tetrahedron, and the glassine is released after deeply penetrating into a corium layer along with a nucleic acid tetrahedron carrier, so that the EGCG can be accurately released to the epidermal layer. The synergistic effects of the three components on whitening and tightening are better exerted.

The invention relates to the technical field of deep processing of cattle spleen, in particular to cattle spleen active polypeptide capable of enhancing immunity and a preparation method and a functional product thereof. The preparation method comprises the following steps: mincing cattle spleen, adding water, uniformly mixing, heating and curing; adding bacillus licheniformis alkaline protease to carry out hydrolysis for the first time; adding papain and bacillus subtilis flavourzyme into the first hydrolysate to carry out hydrolysis for the second time; and performing enzyme deactivation on the second hydrolysate, and sequentially performing fishy smell removal, decolorization, filtration, concentration and drying treatment to obtain the cattle spleen polypeptide. The cattle spleen polypeptide prepared by the invention can obviously enhance the immune response of a body and play a role in enhancing immunity, can be applied to a functional product for enhancing immunity as a functional factor, has the advantages of low equipment requirement, simple process, easiness in operation, high extraction efficiency and the like, is suitable for industrial production, and has a wide application prospect. The scheme of the invention has good application and economic prospects.

The invention discloses a preparation method and permeation promotion application of a high-purity sponge bone needle, and belongs to the technical field of sponge bone needles. Comprising the following steps: cutting a natural sponge into pieces, soaking in deionized water, carrying out ultrasonic oscillation, filtering, and cleaning to obtain a pretreated sponge; carrying out enzymolysis on the pretreated sponge with alkaline protease to obtain a primary zymolyte; and carrying out enzymolysis with ginger protease to obtain a secondary enzymolysis product, and carrying out vacuum freeze drying to obtain the high-purity sponge spicule. The natural sponge is subjected to enzymolysis treatment through alkaline protease and ginger protease, and the high-purity sponge spicule is obtained; wheat oligopeptide is grafted on the surface of a sponge spicule, and giant salamander skin collagen, a pomegranate seed extract, a lonicerae flos extract, a centella asiatica extract, an early celery extract, an iris tectorum extract, an oyster shell extract and calcium chloride mixed treatment materials are loaded, so that the repairing effect on skin inflammation acne is improved.

The invention relates to an application of sea cucumber intestine polysaccharide in preparation of uric acid lowering drugs. The preparation method of the sea cucumber intestine polysaccharide comprises the following steps: performing enzymolysis treatment on sea cucumber intestines by using papain, performing enzyme deactivation, performing centrifugation, and taking supernate; and carrying out graded alcohol precipitation on the supernate, centrifuging, taking precipitate, deproteinizing and freeze-drying. The preparation method is simple, green and environment-friendly. The sea cucumber intestine polysaccharide prepared by the method can effectively inhibit the activity of xanthine oxidase; the sea cucumber intestine polysaccharide has no toxic effect on HK-2 cells. The compound has a uric acid reducing effect on HK-2 hyperuricemia cells.

The invention relates to the technical field of microorganisms, and particularly discloses a fermentation method of bifidobacterium longum BBMN68 as well as a fermented product, a postbiotic and application of the fermented product. According to the method for fermenting the bifidobacterium longum BBMN68, a fermentation substrate comprises alkaline protease hydrolyzed milk and a pomelo peel extract; the hydrolysis rate of the alkaline protease hydrolyzed milk is 9-11%, and the water-soluble dietary fiber content of the shaddock peel extract is 75-80%; the mass ratio of the alkaline protease hydrolyzed milk to the shaddock peel extract is (10-15): (1-3). The content of active ingredients in the fermented product prepared by the method is high, and after the fermented product is prepared into the postbiotics to be taken, the immune function of an organism can be recovered, immune organ damage is reduced, inflammation is relieved, and intestinal flora is regulated and controlled.

The invention discloses a high-transdermal recombinant humanized XVII type collagen as well as a preparation method and application thereof, and belongs to the technical field of synthetic biology. According to the preparation method, transdermal peptide TD-1, different functional fragments of human XVII type collagen and coding genes of His tags are selected to be connected in series, gene sequences are optimized through pichia pastoris codon selection preference firstly, and then the high-transdermal recombinant humanized XVII type collagen is obtained; and then the recombinant humanized XVII type collagen T-COL17-NC16 and the recombinant humanized XVII type collagen T-COL17 are constructed by expression of the recombinant humanized XVII type collagen T-COL17. Effect experiments prove that compared with similar products sold in the market, the skin-care product has more excellent transdermal performance, can improve cell proliferation and migration ability, also has obvious ability of resisting early saccharification product ketoamine and late saccharification product dicarbonyl compounds, better ability of scavenging free radicals and resisting elastase, and can be used for preparing the skin-care product. Moreover, synthesis of melanin in B16-F10 cells can be reduced, and the compound is expected to become a novel functional biological raw material to be applied to the fields of pharmaceutical compositions or skin care products and the like.

OF THE INVENTION Provided herein is a method of preparing an extracellular matrix (ECM) material, including incubating vaginal tissue in a protease, incubating the vaginal tissue in a solution including at least one detergent, incubating the vaginal tissue in a composition including at least one disinfecting agent, thereby producing a decellularized ECM material, lyophilizing the decellularized ECM material, thereby producing a lyophilized ECM material, comminuting the lyophilized ECM material, partially or completely solubilizing the comminuted, lyophilized ECM material with an acid protease to produce solubilized ECM, lyophilizing the solubilized ECM to produce lyophilized, solubilized ECM.

The invention discloses camel milk polypeptide with xanthine oxidase inhibitory activity and application thereof.The camel milk polypeptide is prepared by taking fresh camel milk as a raw material, conducting low-temperature centrifugation to remove fat, conducting triple enzymolysis through papain, pepsin and trypsin to obtain enzymatic hydrolysate, enabling the enzymatic hydrolysate to pass through an ultrafiltration membrane (the molecular weight cutoff is 10 kDa) to obtain filtrate, and conducting freeze-drying to obtain the camel milk polypeptide. The compound has xanthine oxidase inhibitory activity. The invention also discloses a small molecule polypeptide with xanthine oxidase inhibitory activity. The filtered solution is screened and combined with polypeptides by adopting a xanthine oxidase affinity ultrafiltration method, the activity of the polypeptides is verified through molecular docking and in-vitro enzyme inhibition, four small-molecule polypeptides with xanthine oxidase inhibitory activity are obtained, the sequence of the small-molecule polypeptides is shown as any one of SEQ ID NO.1-4, and the small-molecule polypeptides can be used for preparing drugs with the effect of inhibiting the uric acid level.

The invention relates to a PLpro protease small-molecule inhibitor for treating or preventing coronavirus infection and application of the PLpro protease small-molecule inhibitor. Specifically, the invention relates to a compound shown as a formula (I) or an isotope labeled compound thereof, or an optical isomer, a geometric isomer, a tautomer or an isomer mixture thereof, or a pharmaceutically acceptable salt thereof, or a prodrug thereof, or a metabolite thereof, and the use thereof in the preparation of a medicament for treating or preventing coronavirus infection or diseases or symptoms caused by coronavirus.

The invention provides a traditional Chinese medicine fermentation extracting solution and a preparation method and application thereof, and relates to the technical field of traditional Chinese medicine cosmetics. A traditional Chinese medicine composition which is composed of sophora flower bud, clove, honeysuckle, citron, astragalus membranaceus, houttuynia cordata, turmeric, emblic leafflower fruit, rehmannia and roselle and has an anti-aging effect is reasonably combined, and the traditional Chinese medicine fermented extract is obtained after the traditional Chinese medicine composition is fermented for multiple times by utilizing compound probiotics. The traditional Chinese medicine fermentation extract has a strong free radical scavenging effect, has an obvious improvement effect on cell activity, can obviously inhibit matrix protease activity, can obviously improve MDA content in serum of an aging mouse model and indexes of SOD, CAT, GSH-Px activity and the like, and has obvious anti-oxidation and anti-aging effects. The traditional Chinese medicine fermentation extracting solution provided by the invention can be used for preparing anti-aging cosmetics or functional foods, is safe and non-irritant when being used for human skin, and has an obvious improvement effect on the elasticity, wrinkles and smoothness of the human skin.

The invention belongs to the technical field of hair care products, and particularly relates to composite acid gel for scalp care and a preparation method thereof. Salicylic acid, glycolic acid, piroctone azelate olamine salt, echinacea purpurea-lactic acid bacteria yeast, an emulsifier and water are combined, wherein the echinacea purpurea-lactic acid bacteria yeast is prepared by performing enzymolysis on echinacea purpurea through bacillus subtilis protease and then performing aerobic fermentation with lactic acid bacteria. Salicylic acid, glycolic acid and azelaic acid in the composite acid gel are compounded, so that stimulation of salicylic acid is weakened, and the effect of dissolving hair follicle grease is reserved; glycolic acid and azelaic acid are added to accelerate keratin metabolism, regulate sebum secretion and create safe and healthy shielding for the scalp environment, and polysaccharide and flavonoid compounds in the echinacea purpurea-lactic acid bacteria leavening are beneficial to exerting the anti-oxidation and immune regulation effects of the echinacea purpurea-lactic acid bacteria leavening. Therefore, the composite acid gel provided by the invention is green, safe and non-irritant, the preparation method is simple and convenient, and the composite acid gel can be used for inhibiting bacteria and reducing scalp inflammation.

The present invention discloses a natural composite flavor enhancer and a preparation method thereof, comprising the following steps: using an ultramicro grinder to crush rice wine lees and wheat gluten powder to obtain ultramicro wheat gluten powder and ultramicro rice wine lees powder; taking the ultramicro wheat gluten powder, sterile water, and protease, mixing them evenly, carrying out enzymatic hydrolysis, inactivating the enzyme after the reaction ends, filtering, and taking the filtrate, namely the wheat gluten powder enzymatic hydrolysate, for standby; taking the wheat gluten powder enzymatic hydrolysate, ultramicro rice wine lees powder, and sterile water, mixing them evenly, carrying out enzymatic hydrolysis, inactivating the enzyme after the reaction ends, filtering, and taking the filtrate, namely the composite enzymatic hydrolysate, for standby; taking the composite enzymatic hydrolysate, cysteine, xylose, glucose, granulated sugar, and salt, mixing them and carrying out a thermal reaction to obtain a reaction solution; and preparing the natural flavor enhancer by spray drying. The present invention utilizes the natural enzyme system in rice wine lees to synergistically enzymatically hydrolyze wheat gluten powder to promote the release of umami amino acids, and further strengthens the umami by using thermal reaction technology.

The invention discloses novel application of notoginsenoside Rg1, namely application of the notoginsenoside Rg1 in preparation of a cathepsin D (CTSD) inhibitor, the notoginsenoside Rg1 can inhibit CTSD enzyme activity so as to recover lysosome structure and function, relieve endoplasmic reticulum stress reaction and relieve alpha-syn aggregation and dopaminergic neuron apoptosis, has a remarkable protection effect on a Parkinson's disease model, and can be used for preparing a novel anti-inflammatory drug. The invention provides a new thought and a potential drug basis for targeted intervention of Parkinson's disease. The invention discloses a new mechanism that Rg1 plays a role in neuroprotection through a CTSD-lysosome-endoplasmic reticulum stress pathway.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A118 and application thereof. The bacillus velezensis A118 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on colletotrichum capsici. The bacillus velezensis A118 disclosed by the invention also has relatively strong capability of producing cellulase, amylase, protease and indole-3-acetic acid (IAA) and has a relatively good growth promoting effect on chilies, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in a use process and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of pepper diseases is reduced, the pepper growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention provides a preparation method of non-denatured type II collagen capable of increasing bone mineral density and improving bone elasticity, and belongs to the technical field of biologication.The preparation method comprises the steps that an EDTA-citric acid buffer solution containing beta-mercaptoethanol is adopted for decalcifying cartilage, a supercritical CO2 extraction technology is adopted for degreasing, and the collagen is obtained; alkaline protease and collagenase II are adopted for substrate directional enzymolysis and gradient digestion; then the non-denatured type II collagen is separated by adopting three-phase centrifugal separation, and the non-denatured type II collagen with high extraction rate, high purity and high structural integrity can be obtained by adopting bionic H column chromatography purification.

The invention discloses and relates to a strain capable of resisting saline and alkaline and producing IAA (Indoleacetic Acid). The strain is classified and named as bacillus atrophaeus, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.29799. The strain is preserved in China General Microbiological Culture Collection Center on January 29, 2024. The bacillus atrophaeus HHQGTS13-1 can grow under the condition that the salt content is higher than 3%, can generate indoleacetic acid, cellulase, amylase, protease and ligninase, can inhibit various plant pathogenic bacteria, such as banana anthracnose, tomato gray mold, cotton verticillium wilt, rice sheath blight disease, wheat head blight, banana fusarium wilt, capsicum white spot and the like, and can be used for preparing the bacillus atrophaeus HHQGTS13-1. Particularly, the compound plays an important role in preventing and treating wheat scab, banana anthracnose and capsicum white spot. The strain can promote plant growth in a saline-alkali environment, and can be used for improving soil quality, developing special microbial fertilizers, microbial agents and biopesticides for saline-alkali soil, relieving crop salt stress damage and increasing crop yield.

The invention discloses a preparation method of antibacterial and hemostatic medical sponge, and relates to the technical field of preparation of medical sponge. The preparation method of the antibacterial hemostatic medical sponge comprises the following steps: cleaning silkworm cocoons after removing impurities, and immersing the cleaned silkworm cocoons in a neutral protease solution to complete degumming so as to obtain fibroin fibers; the preparation method comprises the following steps: dissolving chitosan in an acetic acid solution, dissolving polyvinyl alcohol in deionized water, adding sodium alginate, adding a silk fibroin solution to form a composite solution, dropwise adding an AgNO3 solution, introducing CO2, maintaining the pressure, quickly releasing the pressure, foaming, injecting into a mold, carrying out gradient freezing to form a multistage-aperture sponge matrix, immersing in a genipin ethanol solution, and transferring into a 5% CaC 12 solution for soaking. The silk fibroin provides a biocompatible substrate and promotes cell adhesion, the sponge is constructed into a hierarchical pore structure with a compact surface and a loose interior, and liquid absorption and blood coagulation factor enrichment are accelerated.

The present application relates to the technical field of oat bran deep processing, and particularly relates to an oat active polypeptide with GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity, and a preparation method and application thereof.The present application is prepared by adding alkaline protease and ficin for double-enzyme step-by-step hydrolysis after homogenate, enzymolysis and alkali extraction and acid precipitation pretreatment, and then filtering, ultrafiltration desalination and drying, so that the oat active polypeptide with GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity is obtained.The present application is simple to operate, low in equipment requirement, and suitable for industrial large-scale production.The prepared oat active polypeptide has GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity, has a significant inhibitory effect on pancreatic lipase and alpha-glucosidase, has an obvious GLP-1 secretion promotion effect, and has a significant blood sugar reduction and lipid reduction effect.

The invention provides a preparation method, application and composition of oat polypeptide, and relates to the technical field of cosmetics. The preparation method disclosed by the invention comprises the following steps: (1) moistening and swelling oat, drying after steam explosion treatment, and adding pectinase, cellulase and amylase to carry out carbohydrate enzymolysis, so as to obtain oat residues after enzymolysis; (2) adding compound protease into the oat residues subjected to enzymolysis, and carrying out primary proteolysis under the condition that the addition amount of ethanol with the volume concentration of 3-8%, so as to obtain enzymolysis slurry A; the compound protease comprises papain, bromelain and flavourzyme in a mass ratio of (3-6): (3-6): 2; (3) adding trypsin into the enzymolysis serous fluid A to carry out secondary proteolysis to obtain enzymolysis serous fluid B; and (4) carrying out ultrafiltration on the enzymolysis slurry B to obtain the oat polypeptide. The obtained oat polypeptide is small in molecular weight and high in content, is used for preparing cosmetics, and has remarkable effects of preserving moisture, resisting wrinkles and reducing skin roughness.

The invention discloses a radix pseudostellariae extract with high content of radix pseudostellariae cyclic peptide B and a preparation method and cosmetic application of the radix pseudostellariae extract, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: (1) crushing radix pseudostellariae, adding alcohol and performing ultrasonic treatment to obtain feed liquid; (2) adding a compound enzyme into the feed liquid to carry out enzymolysis, enzyme deactivation, solid-liquid separation, concentration and impurity removal to obtain an enzymatic hydrolysate; and (3) eluting the enzymatic hydrolysate with macroporous resin to obtain an eluent, and drying to obtain the product, the compound enzyme is prepared from pectinase, papain, beta-glucosidase, cellulase and xylanase according to the mass ratio of (5 to 10) to (0.5 to 1) to (3 to 7) to (0.3 to 1). The radix pseudostellariae extract disclosed by the invention is prepared by adopting an enzymolysis process, more active ingredients can be reserved, the content of radix pseudostellariae cyclic peptide B in the obtained extract is high, and the radix pseudostellariae extract has relatively good anti-inflammatory, soothing, repairing and anti-aging effects.

The invention discloses a preparation method of black garlic polysaccharide, which comprises the steps of extraction, enzymolysis deproteinization, ultrafiltration, concentration, drying, crushing and the like, and'enzymolysis deproteinization + ultrafiltration 'is the core step of the method. Wherein the enzymolysis deproteinization can be selected from papain, neutral protease, acid protease, alkaline protease and the like or a combination of the papain, the neutral protease, the acid protease and the alkaline protease. In the ultrafiltration step, an organic ultrafiltration membrane or an inorganic ceramic membrane or a combination of the organic ultrafiltration membrane and the inorganic ceramic membrane can be selected. The method has the remarkable advantages of simple process flow, low cost, safety, high efficiency, good finished product quality and the like. Pharmacological experiments prove that the black garlic polysaccharide prepared by the method disclosed by the invention has a remarkable anti-constipation effect, and can be used for preparing anti-constipation healthy products, including medicines, health-care foods and foods.

The invention discloses a preparation method of a mesenchymal stem cell exosome based on microcarrier three-dimensional culture and application of the mesenchymal stem cell exosome in anti-aging skin care products. According to the method, a three-dimensional culture system of mesenchymal stem cells is optimized, and the anti-aging function activity of the subsequently separated and purified exosome is remarkably improved by adopting a microcarrier with recombinant collagen as a main component; subsequently, by optimizing an exosome separation and purification technology, and through a specific separation and purification mode and conditions, the exosome with anti-aging active ingredients is further obtained. Experiments prove that the exosome obtained by the method disclosed by the invention can effectively remove metalloproteinase 1 and promote collagen regeneration, has a remarkable anti-aging effect, is suitable for essence, face cream and other dosage forms, and has a wide market prospect.

The invention discloses an application of kaempferol in preparation of a medicine for treating or preventing PEDV (Porcine Epidemic Diarrhea Virus) infection. The kaempferol can obviously inhibit the replication of the PEDV in Vero and LLC-PK1 cells through 3C-like protease targeting the PEDV, so that the kaempferol can be used for preparing the medicine for preventing and treating the PEDV. Besides, kaempferol, as a natural substance separated from mulberries, can be used as a feed additive for a long time so as to achieve the purpose of preventing epidemic diseases, and does not cause generation of drug-resistant strains and other toxic and side effects after being used for a long time; the defects of immune failure possibly caused by vaccination, virus variation caused by use of antiviral drugs and the like are overcome.

The invention belongs to the technical field of marine biological medicines, and relates to a hippocampus polypeptide for improving cognitive impairment of Alzheimer's disease and a preparation method and application thereof.The preparation method comprises the steps that hippocampus powder is dissolved in water, the pH value is adjusted to 1.8-2.5, pepsin is added for oscillation incubation for 2 hours, and then high-temperature heating is conducted to inactivate enzyme; adjusting the pH value to 7.3-8.0, adding chymotrypsin to form a compound enzyme enzymolysis combination, oscillating and incubating for 2 hours, and heating at high temperature to inactivate the enzyme; centrifuging the solution, collecting enzymatic hydrolysate, and filtering the obtained hippocampus crude polypeptide through an ultrafiltration membrane to obtain molecular weight lt; the hippocampus polypeptide is subjected to vacuum freeze drying and preservation. The hippocampus polypeptide extracted and prepared from hippocampus japonicus by adopting a biological enzymolysis method has excellent APP inhibitory activity, can remarkably improve dysmnesia, and can be used for developing related functional foods or medicines for treating Alzheimer's disease.

Disclosed in the present application are a pyridopyrimidinone compound, and a preparation method therefor and the use thereof, wherein the pyridopyrimidinone compound, or a racemate, R-isomer, S-isomer, pharmaceutically acceptable salt thereof or a mixture thereof has a structure as shown in formula (I). The pyridopyrimidinone compound provided by the present application has an agonistic effect on an HsClpP protease, and can be used for treating diseases related to HsClpP receptor activation.

The invention provides a preparation method of a bionic double-layer aerogel thermal fabric, which comprises the following steps: S1, weaving a double-layer texture fabric, selecting superfine polyester as warp yarns and aerogel modified polyester and spandex as weft yarns in a surface texture, and selecting modal fibers as warp yarns and weft yarns in a lining texture; s2, performing low-temperature oxygen plasma treatment on one side of the inner texture of the double-layer texture fabric; s3, soaking the fabric subjected to plasma treatment in a finishing liquid containing cellulase for finishing; s4, after drying the finished fabric, soaking the fabric in a mixed finishing liquid containing protease / lipase for finishing to obtain a protease / lipase finished fabric; and S5, coating one side of the inner tissue of the fabric subjected to protease / lipase finishing with the salt-containing chitosan / waterborne polyurethane finishing liquid, drying, and washing to dissolve out salt, so as to obtain the warm-keeping fabric. According to the invention, material modification and structural design are combined, so that high-efficiency thermal insulation performance is realized.

The invention discloses mandulapalka milk and a preparation method thereof, and belongs to the technical field of beverage processing. According to the technical scheme provided by the invention, the preparation method of the degreased cyperus esculentus milk enzymolysis beverage comprises the following steps: adding high-temperature amylase into a degreased cyperus esculentus meal aqueous solution for enzymolysis for 1 hour, and then adding alkaline protease and flavourzyme for enzymolysis for 1 hour to obtain an enzymolysis solution; and finally, emulsifying residual starch and dextrin in the enzymatic hydrolysate by using soybean protein isolate and corn oil, and homogenizing by using a high-pressure homogenizer to obtain the cyperus esculentus beverage. According to the method, a multi-enzymolysis technology is adopted, the enzymolysis speed can be increased, the taste can be improved, a double-enzymolysis method is adopted, the enzymolysis time is shortened, starch is subjected to enzymolysis more sufficiently, and the flavor is improved. The defatted cyperus esculentus beverage prepared by the method disclosed by the invention is appropriate in sweetness, silky in mouth, mellow and strong in fragrance and unique in flavor, and is a healthy and mellow leisure beverage.