2623 results about "Proteases" patented technology

The invention discloses red ginseng fermentation liquor with high rare saponin content and functional bioactivity and a preparation method thereof, and the preparation method comprises the following steps: mixing red ginseng powder with water to obtain red ginseng mixed liquor; cellulase is added into the red ginseng mixed solution for enzymolysis, and red ginseng enzymatic hydrolysate is obtained; or heating the red ginseng liquid, and performing hot water extraction to obtain a red ginseng extracting solution; heating the red ginseng extracting solution to be boiled, and after sterilization is finished, adding lactic acid bacteria powder for fermentation after the red ginseng solution is cooled, so as to obtain red ginseng fermentation liquor; and carrying out solid-liquid separation on the fermentation broth to obtain a fermentation supernatant, and sterilizing the red ginseng fermentation broth to obtain the sterilized red ginseng fermentation broth. According to the preparation method disclosed by the invention, the content of total saponins in the red ginseng fermentation liquor is increased, the variety and content of rare saponins can be increased, and in addition, the prepared red ginseng fermentation liquor has relatively high antioxidant activity, tyrosinase inhibition rate activity, leukoproteinase inhibition activity and hyaluronidase inhibition activity; wide application prospects and market potentials are realized.

The invention relates to oral liquid for tonifying kidney and bone and a preparation method thereof, and belongs to the technical field of medicine, the oral liquid contains rhizoma drynariae, polygonum multiflorum, poria cocos, teasel root, radix paeoniae alba, angelica sinensis, codonopsis pilosula, prepared rehmannia root, polygonatum kingianum, fructus lycii, native copper and pericarpium citri reticulatae; the rhizoma drynariae is a product obtained by performing enzymolysis on a whole rhizoma drynariae plant through hemicellulase and pectinase, performing enzymolysis through bromelain and papain and performing enzymolysis through nattokinase; polygonum multiflorum is a product obtained by carrying out enzymolysis on Polygonum multiflorum tuberous roots through hemicellulase and pectinase, then carrying out enzymolysis through bromelain and papain and then carrying out enzymolysis through gelatinase. Polygonatum sibiricum is a product obtained by performing enzymolysis on rhizome of Polygonatum sibiricum through hemicellulase and pectinase, performing enzymolysis through bromelain and papain, and performing fermentation through bacillus licheniformis and saccharomyces boulardii. All the active components are matched for use, kidney function metabolism can be well enhanced, bone mineral density is increased, the treatment course is short, the effect taking speed is high, safety and reliability are achieved, and medication compliance is effectively improved.

The invention provides a technical method for improving the anti-aging effect of eurotium cristatum tea compound fermentation liquor, and belongs to the technical field of microorganisms, the Latin name of eurotium cristatum is Eurotium cristatum, the eurotium cristatum is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 2024643. According to the method, the yeast seed solution is inoculated into the tea-sea buckthorn leaf culture solution fermented by the eurotium cristatum, so that the content of anti-aging active components such as procyanidine B2, quercitrin, cyanidin-3-glucoside, nicotinic acid, naringenin and rutin in the fermentation culture solution can be remarkably increased through co-fermentation, and the elastase inhibition rate can be remarkably increased; the anti-aging effect of a component system of the fermentation culture solution is enhanced. The prepared tea-hippophae rhamnoides leaf compound fermentation liquor is suitable for development of anti-aging cosmetics and has a wide market prospect.

The invention provides a high-stability recombinant XVII type collagen as well as a construction method and application thereof, and belongs to the technical field of bioengineering. According to the invention, an amino acid sequence of natural human (XVII) type collagen is selected as a basic unit and repeated for multiple times to obtain recombinant (XVII) type collagen; in the design process of the recombinant XVII type collagen, an unstable GXY triad is avoided, potential matrix metalloproteinase restriction enzyme cutting sites and glycosylation sites are abandoned, a KGD active sequence is reserved, and the probability of containing sites which are easy to degrade can be reduced; a sequence fragment of the recombinant XVII type collagen is derived from a full-length sequence of a natural collagen gene, has excellent solubility, biological activity and high stability, and can be kept stable in a high-temperature storage environment and under the condition of avoiding alkalinity; the product can be used as a raw material in the production process of medicines, medical equipment, biological materials or cosmetics, and has good practicability.

The invention relates to a weight-losing enzyme and a preparation method thereof, and belongs to the technical field of biology, and the weight-losing enzyme comprises a fruit enzyme, a vegetable enzyme, a seed enzyme and a lotus leaf extract; the fruit enzyme is obtained by performing enzymolysis on mixed fruit pulp of apples, lemons, grapefruits and haws through pectinase, performing enzymolysis through pepsase and performing co-fermentation through bifidobacterium longum and lactobacillus acidophilus; the vegetable enzyme is obtained by performing enzymolysis on mixed vegetable pulp of wax gourd pulp, celery and spinach through pectinase, performing enzymolysis through pepsin and performing fermentation through lactobacillus plantarum and bifidobacterium adolescentis; the seed enzyme is obtained by carrying out trypsin enzymolysis on mixed powder of semen raphani and phaseolus calcaratus, then carrying out nattokinase enzymolysis and then fermenting with bifidobacterium breve; the lotus leaf extract is obtained by sequentially carrying out enzymolysis on lotus leaves through hemicellulase, snail protease and nattokinase. All the components are matched for use, so that the weight-losing food has the effects of promoting digestion, inhibiting fat absorption, regulating intestinal flora, promoting metabolism and the like, and has a good weight-losing effect.

The invention provides bacillus paralicheniformis NJ102 and application thereof, and belongs to the technical field of microorganisms. The bacillus paralicheniformis NJ102 disclosed by the invention has the capabilities of resisting low temperature and saline and alkaline, and can endure the low temperature of 10 DEG C, the salt stress with the salt content of 17% and the strong alkali stress with the pH value of 12.5. Meanwhile, the bactericidal composition has a relatively good inhibition effect on ralstonia solanacearum, rhizoctonia solanacearum and phytophthora capsici, and can effectively prevent and treat tomato bacterial wilt, root rot and fruit rot. The bacillus paralicheniformis disclosed by the invention also has relatively strong capability of producing protease, cellulase, amylase and IAA (Indoleacetic Acid), has a relatively good growth promoting effect on tomatoes, and has great application potential in the aspect of biopesticide.

The invention relates to a stable silk fibroin liquid condensation body, gel and a preparation method and application thereof, and belongs to the technical field of silk fibroin. The preparation method comprises the following steps: S1, dissolving silk fibroin in a lithium bromide solution, and then adding trypsin for incubation to obtain an enzyme-digested silk fibroin solution; and S2, performing low-temperature programmed centrifugal treatment including an induction stage, a nucleation stage and a liquid-liquid phase separation stage on the enzyme-digested silk fibroin solution, and cleaning to obtain the stable silk fibroin liquid-state aggregate, the method can reduce aggregate liquid drop aggregation and improve the particle size uniformity of the liquid drops.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis F0b and an application of the bacillus velezensis F0b. The bacillus velezensis F0b is preserved in the Guangdong Microbial Culture Collection Center on April 26, 2024, the preservation date of the bacillus velezensis F0b is April 26, 2024, and the preservation number of the bacillus velezensis F0b is GDMCC (China General Microbiological Culture Collection Center): 64566. The bacillus velezensis has prevention and / or control effects on pyricularia oryzae, cabbage sclerotinia sclerotiorum, tomato fusarium wilt, banana fusarium wilt, rice leaf spot, peronophythora litchii, soybean carbon rot, colletotrichum capsici and corn leaf curvularia, and can be used for preparing broad-spectrum microbial source bactericides. The fermentation liquor of the bacillus velezensis can generate amylase, carboxymethyl cellulose and protease, and the fermentation liquor of the bacillus velezensis has prevention and / or control effects on vegetable pathogenic fungal diseases, namely cabbage sclerotiniose or pepper anthracnose.

The invention provides bacillus velezensis NR10, and the preservation number of the bacillus velezensis NR10 is CGMCC (China General Microbiological Culture Collection Center) NO.31771. The bacillus velezensis NR10 is a strawberry endophyte, has good bacteriostatic activity, has good antagonistic action on botrytis cinerea, fusarium oxysporum, colletotrichum gloeosporioides, colletotrichum oxysporum, trichoderma harzianum, trichoderma longibrachiatum and the like, is a potential biological preparation for preventing and treating strawberry diseases and the like, and has broad application prospects. And the bacteriostatic action of the bacillus velezensis NR10 has thermal stability. In addition, the bacillus velezensis also has good capacities of producing siderophores, fixing nitrogen and dissolving potassium, producing protease and cellulase and the like. The bacillus velezensis NR10 provided by the invention has wider-spectrum disease resistance, wide development space and good development and application prospects.

Described are RNAi agents, compositions that include RNAi agents, and methods for dual inhibition of an Apolipoprotein C-III (APOC3) and Proprotein Convertase Subtilisin Kexin 9 (PCSK9) gene. The APOC3-PCSK9 RNAi agents disclosed herein inhibit the expression of an APOC3 and a PCSK9 gene. Pharmaceutical compositions that include one or more APOC3-PCSK9 RNAi agents, optionally with one or more additional therapeutics, are also described. Delivery of the described TSLP RNAi agents to hepatic cells, in vivo, provides for inhibition of APOC3 and / or PCSK9 gene expression, which can provide a therapeutic benefit to subjects, including human subjects, for the treatment of various diseases including hypertriglyceridemia and hypercholesterolemia.

The present disclosure relates to proteases that exhibit activity against immunoglobulins, particularly IgM antibodies, and uses of the proteases for analysis of IgM, for generation of IgM antibody fragments, diagnostics related to IgM antibodies, and for treating and / or preventing diseases mediated by IgM antibodies.

The present disclosure relates to a prediction for the influence of a mutation on protease activity. A method for predicting the influence of a mutation on protease activity comprises: on the basis of sequence information of a protease sample to be subjected to prediction, performing feature extraction by using a pre-training model, so as to obtain a sequence feature of said protease sample (S110); on the basis of three-dimensional structure information of said protease sample, obtaining a structural feature of said protease sample (S120); and on the basis of the sequence feature and the structural feature, performing prediction by using a prediction model based on a graph neural network, and determining an activity change prediction result after said protease sample mutates (S130). Further disclosed is a method for constructing a prediction model used for predicting the influence of a mutation on protease activity. By means of the method in the present disclosure, prediction is performed on the basis of an advanced natural language processing model and a graph neural network, in combination with a sequence and a three-dimensional structure, and by means of an artificial intelligence model, showing a performance superior to that of other existing methods, and proving the wide application prospects of the method in protease engineering.

The invention discloses application of a low-toxicity broad-spectrum antibacterial peptide taking WW as a symmetric center, WK is repeated twice and symmetrically distributed on two sides, WW is taken as the symmetric center to obtain an antibacterial peptide WKWKWWKWKW-NH2 (N2W2), and tryptophan at the C terminal and the N terminal of the N2W2 is replaced with leucine and / or isoleucine to obtain the antibacterial peptide. The antibacterial peptide can basically maintain the excellent antibacterial activity of the parent peptide, and the hemolytic activity of the antibacterial peptide is greatly reduced, so that the safety is improved. Wherein the antibacterial activity of IL-N2W2 is the highest, but the stability of IL-N2W2 needs to be improved, so that D-IL-N2W2 is obtained by carrying out D-type amino acid substitution on IL-N2W2. The D-IL-N2W2 keeps the high antibacterial activity and low toxicity of IL-N2W2, can still completely inhibit the growth of bacteria after being incubated with high-concentration trypsin or chymotrypsin, has great potential in the aspect of relieving the drug resistance of antibiotics, and has good application prospects in preparation of clinical antibacterial drugs.

The invention discloses staphylococcus NHY-25 capable of producing enzyme and aroma, which is preserved in Guangdong Microbial Culture Collection Center on May 26, 2025, and the preservation number is GDMMC NO: 66396. When the strain is applied to yeast strengthening, the enzyme activity in high-temperature yeast can be improved, for example, the activity of protease, saccharifying power, fermenting power and liquefying power of yeast can be improved. Through detection of volatile compounds in the strengthened Daqu and the unstrengthened Daqu, it is found that the strain enables the variety and content of the volatile compounds in the strengthened Daqu to be improved. The strain enables the sauce flavor of high-temperature yeast to be more prominent, can be applied to yeast strengthening, and provides a new thought for improving the quality of yeast.

The invention belongs to the technical field of cosmetics, and discloses a composition with tightening and anti-wrinkle effects as well as a preparation method and application thereof. The composition disclosed by the invention comprises the following components: a radix pseudostellariae extract and hydrolyzed ginsenoside. The radix pseudostellariae extract in the composition achieves the effects of tightening and resisting wrinkles by promoting generation of collagen and elastin, the hydrolyzed ginsenoside mainly has the effects of resisting oxidation, inhibiting expression of matrix metalloproteinase and preventing collagen degradation, and after the radix pseudostellariae extract and the hydrolyzed ginsenoside are combined, the composition has a better effect of inhibiting the activity of the matrix metalloproteinase, so that the effect of resisting wrinkles is achieved. The traditional Chinese medicine composition has the effects of promoting the generation of collagen and elastin, and has a synergistic effect, so that the traditional Chinese medicine composition has an excellent anti-aging effect.

The invention discloses a tightening and whitening composition containing EGCG (epigallocatechin gallate) as well as a preparation method and application of the tightening and whitening composition, and relates to the technical field of cosmetics. The invention provides a tightening and whitening composition containing EGCG (epigallocatechin gallate). The tightening and whitening composition is prepared from the following components in parts by weight: 0.1 to 2 parts of an epigallocatechin gallate nano composition, 0.05 to 0.5 part of a nucleic acid tetrahedron and 0.5 to 5 parts of glassine. When the EGCG nano composition, the nucleic acid tetrahedron and the glassine are cooperatively used, the stability of a formula can be improved, meanwhile, the EGCG can be accurately released to an epidermal layer due to the enzyme-responsive degradation characteristic (such as matrix metalloproteinase MMP-2 triggered depolymerization) of the nucleic acid tetrahedron, and the glassine is released after deeply penetrating into a corium layer along with a nucleic acid tetrahedron carrier, so that the EGCG can be accurately released to the epidermal layer. The synergistic effects of the three components on whitening and tightening are better exerted.

The invention discloses a large-model-driven intelligent biosynthesis system and method, and the system employs a centralized multi-large-model intelligent agent architecture, and executes a task through a modularized intelligent agent. The system is composed of a man-machine cooperation interaction module, a global task management module, a general information retrieval module, a reverse path planning module, a protease modification module, a metabolic pathway optimization module, a wet experiment tool execution module and a code execution module. Each module integrates different tools and data sources for specific task fields. By means of the system, complex biosynthesis experiment design, planning and execution can be completed autonomously, functional modules and wet experiment feedback can be dynamically adapted, and the flexibility of experiment planning is ensured.

The invention relates to the technical field of deep processing of cattle spleen, in particular to cattle spleen active polypeptide capable of enhancing immunity and a preparation method and a functional product thereof. The preparation method comprises the following steps: mincing cattle spleen, adding water, uniformly mixing, heating and curing; adding bacillus licheniformis alkaline protease to carry out hydrolysis for the first time; adding papain and bacillus subtilis flavourzyme into the first hydrolysate to carry out hydrolysis for the second time; and performing enzyme deactivation on the second hydrolysate, and sequentially performing fishy smell removal, decolorization, filtration, concentration and drying treatment to obtain the cattle spleen polypeptide. The cattle spleen polypeptide prepared by the invention can obviously enhance the immune response of a body and play a role in enhancing immunity, can be applied to a functional product for enhancing immunity as a functional factor, has the advantages of low equipment requirement, simple process, easiness in operation, high extraction efficiency and the like, is suitable for industrial production, and has a wide application prospect. The scheme of the invention has good application and economic prospects.

The invention discloses a preparation method and permeation promotion application of a high-purity sponge bone needle, and belongs to the technical field of sponge bone needles. Comprising the following steps: cutting a natural sponge into pieces, soaking in deionized water, carrying out ultrasonic oscillation, filtering, and cleaning to obtain a pretreated sponge; carrying out enzymolysis on the pretreated sponge with alkaline protease to obtain a primary zymolyte; and carrying out enzymolysis with ginger protease to obtain a secondary enzymolysis product, and carrying out vacuum freeze drying to obtain the high-purity sponge spicule. The natural sponge is subjected to enzymolysis treatment through alkaline protease and ginger protease, and the high-purity sponge spicule is obtained; wheat oligopeptide is grafted on the surface of a sponge spicule, and giant salamander skin collagen, a pomegranate seed extract, a lonicerae flos extract, a centella asiatica extract, an early celery extract, an iris tectorum extract, an oyster shell extract and calcium chloride mixed treatment materials are loaded, so that the repairing effect on skin inflammation acne is improved.

The invention provides an anti-aging and whitening pyracantha fortuneana fruit extract prepared by adopting a supramolecular solvent and a preparation method of the pyracantha fortuneana fruit extract, and relates to the technical field of biological extraction. The preparation method comprises the following steps: by taking betaine as a hydrogen bond acceptor and 1, 3-propylene glycol as a hydrogen bond donor, mixing and self-assembling the betaine and the 1, 3-propylene glycol to form a supramolecular solvent; and the pyracantha fortuneana fruits are extracted by adopting the supramolecular solvent. The pyracantha fortuneana fruit active ingredients are extracted by adopting the supramolecular solvent which is harmless to the skin, so that the irritation of the solvent to the skin can be avoided, the supramolecular solvent can be specifically combined with the active ingredients in the pyracantha fortuneana fruit, and the purity and activity of the active ingredients are improved. The pyracantha fortuneana fruit extract extracted by adopting the supramolecular solvent has better oxidation reduction resistance; generation of non-enzymatic glycosylation intermediate products is inhibited, and saccharification and aging resistance are facilitated; the red blood cell oxidative damage is improved, and the effects of resisting oxidation and relieving are achieved; the whitening mask has an inhibiting effect on elastinase and tyrosinase, and can resist aging, whiten skin, relieve facial pigmentation and brighten the skin.

The invention provides an anti-pilling wool fabric and a treatment method thereof, and belongs to the technical field of wool fabric processing. According to the treatment method for improving the anti-pilling performance of the wool fabric, the wool fabric with the anti-pilling performance is obtained, and the problems that in the prior art, in order to prevent pilling of the wool fabric, protease is used for treating the wool fabric, the efficiency is too low, and industrialization cannot be achieved are solved. Through the synergistic effect of the bacterial cellulose and the sericin, the anti-pilling performance of the wool fabric is improved, and the method is easy and convenient to operate, high in efficiency and suitable for industrial production.

The invention discloses a multifunctional peoriae paenibacillus and application thereof, and relates to the technical field of microorganisms, and the key point of the technical scheme is as follows: Paenibacillus peoriae S6330 is separated from rhizosphere soil; the peoriae paenibacillus has a good inhibition effect on pathogenic bacteria, pathogenic fungi and oomycetes; meanwhile, the peoriae paenibacillus has the functions of dissolving potassium, producing protease, producing amylase, producing cellulase, producing siderophore and the like, has a growth promoting effect on various crops such as wheat, corn, watermelons, small oilseed rapes, tobaccos and the like, and can remarkably improve indexes such as chlorophyll content, plant height, fresh weight, overground part weight, underground part weight and the like of plants; in addition, the peoriae paenibacillus can be used for effectively inhibiting the tobacco bacterial wilt; the peoriae paenibacillus has no environmental pollution problem, is simple in culture condition, and has wide disease prevention and growth promotion application prospects in agricultural production.

The invention discloses a protease preparation for preventing pet hair ball disease as well as a preparation method and application thereof, and belongs to the technical field of pet nutrition and health. The protease preparation for preventing the pet hair ball disease is mainly prepared from a compound enzyme, vitamins, trace elements, dietary fibers, probiotics, meat powder and a wall material, and the mass ratio of the compound enzyme to the vitamins to the trace elements to the dietary fibers to the probiotics to the meat powder to the wall material is (1-100): (0.1-1): (0.1-10): (1-70): (0.1-10): (10-60): (10-60). The protease preparation provided by the invention can effectively degrade hair and indigestible food, prevents the hair and food residues from forming pellets to block gastrointestinal tracts, prevents hair bulb syndrome from occurring in mechanism, and is simple in preparation process and wide in application prospect.

The invention relates to an application of sea cucumber intestine polysaccharide in preparation of uric acid lowering drugs. The preparation method of the sea cucumber intestine polysaccharide comprises the following steps: performing enzymolysis treatment on sea cucumber intestines by using papain, performing enzyme deactivation, performing centrifugation, and taking supernate; and carrying out graded alcohol precipitation on the supernate, centrifuging, taking precipitate, deproteinizing and freeze-drying. The preparation method is simple, green and environment-friendly. The sea cucumber intestine polysaccharide prepared by the method can effectively inhibit the activity of xanthine oxidase; the sea cucumber intestine polysaccharide has no toxic effect on HK-2 cells. The compound has a uric acid reducing effect on HK-2 hyperuricemia cells.

The invention relates to the technical field of microorganisms, and particularly discloses a fermentation method of bifidobacterium longum BBMN68 as well as a fermented product, a postbiotic and application of the fermented product. According to the method for fermenting the bifidobacterium longum BBMN68, a fermentation substrate comprises alkaline protease hydrolyzed milk and a pomelo peel extract; the hydrolysis rate of the alkaline protease hydrolyzed milk is 9-11%, and the water-soluble dietary fiber content of the shaddock peel extract is 75-80%; the mass ratio of the alkaline protease hydrolyzed milk to the shaddock peel extract is (10-15): (1-3). The content of active ingredients in the fermented product prepared by the method is high, and after the fermented product is prepared into the postbiotics to be taken, the immune function of an organism can be recovered, immune organ damage is reduced, inflammation is relieved, and intestinal flora is regulated and controlled.

The invention discloses a strain of lactobacillus plantarum, which is named as L.plantarum ZW1, and the preservation number of the lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) 26685. The lactobacillus plantarum provided by the invention is a lactic acid bacterium with relatively strong broad-spectrum inhibition capability, and after the interference of organic acid and hydrogen peroxide is eliminated and protease treatment verification is carried out, the strain is determined to have relatively strong bacteriocin production capability. Probiotic performance evaluation shows that the strain has good digestive tract fluid tolerance, and the L.plantarum CGMCC26685 has good intestinal tract probiotic characteristics, can significantly enrich mouse intestinal flora in an in-vitro intestinal flora fermentation model, increase the relative abundance of the phylum of thick-walled bacteria, reduce the relative abundance of the phylum of proteobacteria, proteusbacillus and shigella, and improve the intestinal tract fluid resistance of mice. The number of flora OTU is obviously higher than that of lactobacillus rhamnosus LGG and lactobacillus plantarum L.plantarum PL4 group which does not produce bacteriocin, and the Chao1 index, the Shannon index and the Simpson index are obviously improved compared with those of a control group. It is shown that the L.plantarum CGMCC26685 has good intestinal tract beneficial activity, and has the potential of further performance excavation and application expansion.

The invention discloses a high-transdermal recombinant humanized XVII type collagen as well as a preparation method and application thereof, and belongs to the technical field of synthetic biology. According to the preparation method, transdermal peptide TD-1, different functional fragments of human XVII type collagen and coding genes of His tags are selected to be connected in series, gene sequences are optimized through pichia pastoris codon selection preference firstly, and then the high-transdermal recombinant humanized XVII type collagen is obtained; and then the recombinant humanized XVII type collagen T-COL17-NC16 and the recombinant humanized XVII type collagen T-COL17 are constructed by expression of the recombinant humanized XVII type collagen T-COL17. Effect experiments prove that compared with similar products sold in the market, the skin-care product has more excellent transdermal performance, can improve cell proliferation and migration ability, also has obvious ability of resisting early saccharification product ketoamine and late saccharification product dicarbonyl compounds, better ability of scavenging free radicals and resisting elastase, and can be used for preparing the skin-care product. Moreover, synthesis of melanin in B16-F10 cells can be reduced, and the compound is expected to become a novel functional biological raw material to be applied to the fields of pharmaceutical compositions or skin care products and the like.

OF THE INVENTION Provided herein is a method of preparing an extracellular matrix (ECM) material, including incubating vaginal tissue in a protease, incubating the vaginal tissue in a solution including at least one detergent, incubating the vaginal tissue in a composition including at least one disinfecting agent, thereby producing a decellularized ECM material, lyophilizing the decellularized ECM material, thereby producing a lyophilized ECM material, comminuting the lyophilized ECM material, partially or completely solubilizing the comminuted, lyophilized ECM material with an acid protease to produce solubilized ECM, lyophilizing the solubilized ECM to produce lyophilized, solubilized ECM.

The invention discloses camel milk polypeptide with xanthine oxidase inhibitory activity and application thereof.The camel milk polypeptide is prepared by taking fresh camel milk as a raw material, conducting low-temperature centrifugation to remove fat, conducting triple enzymolysis through papain, pepsin and trypsin to obtain enzymatic hydrolysate, enabling the enzymatic hydrolysate to pass through an ultrafiltration membrane (the molecular weight cutoff is 10 kDa) to obtain filtrate, and conducting freeze-drying to obtain the camel milk polypeptide. The compound has xanthine oxidase inhibitory activity. The invention also discloses a small molecule polypeptide with xanthine oxidase inhibitory activity. The filtered solution is screened and combined with polypeptides by adopting a xanthine oxidase affinity ultrafiltration method, the activity of the polypeptides is verified through molecular docking and in-vitro enzyme inhibition, four small-molecule polypeptides with xanthine oxidase inhibitory activity are obtained, the sequence of the small-molecule polypeptides is shown as any one of SEQ ID NO.1-4, and the small-molecule polypeptides can be used for preparing drugs with the effect of inhibiting the uric acid level.

The invention relates to a PLpro protease small-molecule inhibitor for treating or preventing coronavirus infection and application of the PLpro protease small-molecule inhibitor. Specifically, the invention relates to a compound shown as a formula (I) or an isotope labeled compound thereof, or an optical isomer, a geometric isomer, a tautomer or an isomer mixture thereof, or a pharmaceutically acceptable salt thereof, or a prodrug thereof, or a metabolite thereof, and the use thereof in the preparation of a medicament for treating or preventing coronavirus infection or diseases or symptoms caused by coronavirus.