67 results about "Collagen degradation" patented technology

The invention provides a method for determining the efficacy of a TNFα inhibitor, such as a TNFα antibody, or an antigen-binding portion thereof, for treating ankylosing spondylitis (AS), using a collagen degradation biomarker and / or a synovitis biomarker.

The invention provides a method for determining the efficacy of a TNFα inhibitor, such as a TNFα antibody, or an antigen-binding portion thereof, for treating ankylosing spondylitis (AS), using a collagen degradation biomarker and / or a synovitis biomarker.

Biomarkers for diagnosis and monitoring of vascular aneurysms are described in the context of the use of assays to measure a plurality of these biomarkers. Tissue degeneration, particularly elastin and / or collagen degradation, can be monitored within patient blood (serum) and / or urine to diagnose the presence, the progression, or the likelihood of rupture of aneurismal disease. Additionally, enzymes responsible for this degradation and other biomarkers responsible for the activation or inhibition of these enzymes can be monitored additionally or alternatively. Prompt diagnosis can provide the opportunity for intervention and potentially increase the health of patients by tempering the development of debilitating and life-threatening vascular aneurysms.

The invention belongs to the technical field of medicines, discloses applications of andrographolide derivatives in preparation of medicines preventing and treating hepatic fibrosis, and relates to 15-benzylidene-14-deoxy-11,12-dehydroandrographolide derivatives and 3,19 esterified compounds thereof. Experiments prove that the compounds significantly inhibit human hepatic stellate cell LX-2 metastasis and activation, significantly reduce the fibrosis level of hepatic tissues of rats affected with hepatic fibrosis, reduce contents of extracellular matrix protein (ECM) related components, significantly reduce the level of immune inflammation correlation factors of rats affected with hepatic fibrosis, effectively inhibit immuno-inflammatory responses, inhibit hepatic stellate cell activation in hepatic tissues, and promote collagen degradation. The compounds are used as active components for preparing the anti-hepatic fibrosis medicines, and are efficient and low in toxicity, thus providing a novel medicine route for hepatic fibrosis treatment and prevention, and expanding the optional range of clinical medicine application. The compounds and the applications have good development prospects.

Polyelectrolyte based bioactive super-absorbent material ionotropically crosslinked / neutralized involving polyvalent carboxylic acids, citrate, Kojic Acid, Alpha Arbutin along with fish scale collagen cross linked with other polyelectrolyte biopolymers preferably selected from chitosan, alginate or their combinations is used in this invention. The advancement is also directed to process of extraction of collagen of high purity from fresh water fish scale by salt and alkaline washing, crushing followed by continuous dialysis thereby minimizing the chance of collagen degradation. Also disclosed are different forms of collagen-chitosan composite biomaterial using citrate as the neutralizing buffer in combination with antimicrobial agent, antioxidant, skin plumper and melanin reducer wherein the different forms of collagen chitosan particularly sheet, flakes, powder, gel, particles, fiber, film, spray etc. reveal efficient wound healing properties. The advancement is thus directed to find wide application in various dermal wound healing, tissue engineering, 3D cell culture, cell expansion and cell delivery vehicle, mimicking the in vivo situation in dynamic condition, cosmetics and different other health care applications.

The use of a collagenase containing formulation for degrading collagen within an occlusive atherosclerotic plaque in a chronic fibrotic occlusion, chronically occluded animal tube or cavity. A medical-related apparatus is provided comprising a medical-related device having provided thereto a therapeutic amount of a collagen degrading composition comprising a proteiolytic enzyme containing formulation. A method is provided for treating chronically occluded animal tubes and cavities by administering a therapeutic effective amount of a proteolytic enzyme-containing formulation adjacent to an occluding atherosclerotic plaque, waiting for a pre-angioplasty waiting period, followed by crossing the plaque with an angioplasty guide wire.

The invention discloses a degrading collagen-polyurethane waterborne wood adhesive, which is prepared from the following ingredients in parts by weight: 100 to 150 parts of isocyanate, 50 to 75 parts of polyester type polyalcohol, 0.1 to 0.2 part of catalysts, 30 to 45 parts of hydrophilic extended chain agents, 10 to 15 parts of micromolecule diglycol chain extenders, 20 to 30 parts of neutralizing agents and 50 to 110 parts of degradation collagen. The invention also discloses a preparation method of the degradation collagen-polyurethane water-based wood adhesive. A chrome shaving waste degradation product generated in a leather production process is used as a raw material; a path is increased for the resource reutilization of the chrome shaving waste; the corresponding economic benefits are brought; meanwhile, the environment pressure is relieved to a certain degree. Meanwhile, through the copolymerization modification with waterborne polyurethane, the problem of high cost of the single polyurethane is solved; meanwhile, the adhesive intensity, the water-resistant performance and the like of the chrome shaving collagen degradation substances are effectively improved.

The invention relates to an injection (intracutaneous injection) for correcting skin wrinkles and a preparation method thereof. The injection is prepared from the following components by mass: 3-6g / L of high molecular polysaccharide, 50-200mg / L of amino acid, 2-10mg / L of water-soluble vitamin and 2-5g / L of L-carnosine. A sample prepared according to the formula is capable of supplying extracellular matrix to skin and promoting synthesis of spontaneous collagen of skin. The injection has the function of a traditional filling material for supplying lost extracellular matrix, is capable of promoting synthesis of collagen and solving the problem of wrinkled and loose skin caused be senility. Furthermore, compared with a conventional filling material, the injection is lower in viscosity and is more suitable for intracutaneous injection. According to the injection prepared by the method, the problem of sodium hyaluronate and collagen degradation of skin in the aging process can be solved by means of the intracutaneous injection, and an anti-wrinkle and skin-firming effect can be achieved.

The present invention relates to a method for monitoring effects of administration of a parathyroid hormone by determining levels of one or more markers of an activity of this hormone. Suitable markers of bone formation include one or more enzymes indicative of osteoblastic processes of bone formation, preferably bone specific alkaline phosphatase, and / or one or more products of collagen biosynthesis, preferably a procollagen I C-terminal propetide. Suitable markers of bone resorption and turnover include one or more products of collagen degradation, preferably an N-terminal telopeptide (NTX). In addition, methods for concurrently reducing the risk of both vertebral and non-vertebral bone fracture in a male human subject at risk of or having ossteoporosis are also disclosed, involving administration of human parathyroid hormone (amino acid sequence 1-34) without concurrent administration of an antiseropositive agent other than vitamin D or calcium.

The invention relates in particular to compaction eye cream and belongs to the field of skin care products. The compaction eye cream is rich in total panax notoginseng saponin and a cynara scolymus leaf extract which have a synergistic effect to effectively remove skin inflammation, allow nutrition channels of skin to be smooth and lay a solid foundation for subsequent absorption of nutrient substances; palmitoyl tripeptide-5, nicotinamide and D-panthenol have a synergistic effect to avoid collagen degradation, promote collagen regeneration and allowthe skin around eyes to be plump and compact; and the eye cream also comprises active ingredients such as mango seed fat, meadowfoam seed oil, beewax vitamin E acetate, and micromolecular sodium hyaluronate. The eye cream can effectively removethe eye skin inflammation, deeply supplement nutrition for the eye skin, effectively improve blood circulation of the eye skin, regenerate collagen, gradually remove wrinkles, and allow the eye skinto be plump, compact and moist.

The invention discloses a collagen peptide with the function of promoting bone growth as well as a preparation method and an application thereof. The collagen peptide is obtained from a fish skin collagen matrix through impurity removal, collagen degradation, clarification, concentration and drying. The method disclosed by the invention determines a collagen peptide for promoting bone growth by promoting bone cell proliferation and differentiation through screening and control of an enzyme condition and activity verification. The collagen peptide processed by using the method is uniform in product colour, fine in powder, good in solubility, free from a fishy smell and a peculiar smell, high in activity of promoting bone cell proliferation and differentiation, and good in application prospect in the field of food processing and the aspect of nutritious and nourishing health foods.

The invention relates to the technical field of application of a cistanche phenylethanoid glycosides extract, and discloses the application of a cistanche phenylethanoid glycosides extract in preparing drugs for controlling hepatic fibrosis for the first time. The anti-hepatic fibrosis effect of the cistanche phenylethanoid glycosides extract is remarkable, activation and proliferation of hepatic stellate cells can be restrained, collagen synthesis can be reduced, collagen degradation can be promoted, the content of collagen in hepatic fibrosis tissue and the content of hydroxyproline in hepatic tissue can be reduced, and then synthesis of the extracellular matrix is restrained, the anti-hepatic fibrosis function is realized and a new direction is provided for controlling hepatic fibrosis diseases.

The present invention relates to methods and kits for evaluating the severity of a burn injury, which are based on the detection in a clinical fluid sample of skin metabolism products, such as collagen peptides which are released upon collagen degradation or synthesis.

The invention relates to a preparation method of a sand-fixing water-retention agent, particularly a preparation method of a collagen-degradation-product-based sand-fixing water-retention agent, which comprises the following steps: cleaning and drying tanning shaving waste scraps, pulverizing, treating with a white lime solution at 85-90 DEG C, regulating the pH value to neutrality with sulfuric acid, decolorizing, flocculating, and carrying out liquid-solid pressure filtration separation to obtain a collagen hydrolysate; heating the collagen hydrolysate to 75-85 DEG C, dropwisely adding vinyl monomer and 4 / 5 of initiator, heating to 80-90 DEG C, adding the rest of initiator, keeping the temperature to react for 1-1.5 hours, and stopping heating; and cooling to 40-45 DEG C, and regulating the pH value to 6.5-7.0 to obtain the sand-fixing water-retention agent. The method regenerates and recovers leather wastes. The prepared sand-fixing water-retention agent has the advantages of favorable water solubility, high storage stability, light fastness and thermal aging resistance, and can form a bonding layer when being sprayed to the sand grain surface, thereby enhancing the wind erosion resistance and compression strength of the sand grains and achieving the goal of san fixation.

The present invention relates to an anti-wrinkle cosmetic composition comprising chitooligosaccharide as an effective component, more specifically, the cosmetic composition to inhibit collagen degradation induced by UV-A irradiation.

The invention belongs to the field of gelatin preparation, in particular to a method for enzymatically degrading bone collagen to prepare gelatin. (1) Use degreased and non-demineralized aggregates to mix with water and grind them into bone slurry, and the bone particle size is controlled at 1-5mm; (2) Adjust the pH value to 1.5-4 with acid, or adjust the pH value to 7-8 with alkali, Add acid or partial alkaline protease to control collagen degradation; the amount of protease added is 2-8‰ of the bone mud weight; (3) react at room temperature for 7-10 hours, adjust the pH of the solution to 5.0-6.5 with alkali or acid , heating the temperature to 70 to 85°C for extraction; (4) separating the turbid gelatin solution obtained from the extraction; (5) filtering the glue solution to obtain a high-purity hard capsule gelatin solution. The dried gelatin is tested, and the molecular weight distribution of gelatin is α=48%, β=18%; the jelly strength is over 240 grams; the viscosity is 3.0mPs; the transmittance is 90% at 620nm and 71% at 450nm.

The invention discloses a detection kit for urine beta-collagen degradation products. The detection kit mainly comprises microwell plates coated with CTX-I antigens and a rabbit anti CTX antibody solution, wherein the amount of the coated CTX-I antigens of each microwell plate is 0.48-7.2 mug, and the valence of rabbit anti CTX antibodies achieves 1:10000. The detection kit for the urine beta-collagen degradation products has the advantage of good specificity, sensitivity and precision.

The present invention relates to a cosmetic composition containing a ginseng fruit fermented material obtained by double fermentation of ginseng fruits with Aureobasidium pullulans CXHB-8 and lactic acid bacteria. According to the present invention, a cosmetic agent containing a ginseng fruit fermented material obtained by double fermentation of ginseng fruits with Aureobasidium pullulans CXHB-8 and lactic acid bacteria can suppress skin aging and relieve wrinkles by promoting cell proliferation, recovering damaged cells, and inhibiting collagen degradation.

The invention relates to a Chenopodiumformosanum Koidz (CFK) extract with anti-aging, whitening, anti-allergic and cell repair functions, which is to administer an effective dose of CFK extract to skin cells. The invention has mechanism functions of DPPH and ABTS +free radical removal ability, reducing power, chelation of iron ions and inhibition of lipid peroxide efficiency; inhibition of tyrosinase activity; removal of nitric oxide (NO) free radicals, and inhibition of lipoxygenase (LOX-1) activity; inhibition of hyaluronidase, increase in the hyaluronidase content of the skin, promotion ofwound repair, and reduction of the production of wrinkles; inhibition of the activity of matrix metalloproteinases (MMPs), and reduction of the degradation of collagen; and promotion of the ability of cell repair, enhancement of the anti-allergic ability and the like.

0Type II collagen degradation is measurable using a sandwich immunoassay in which a single antibody specific for the amino acid sequence EKGPDP is used to form each side of antibody-collagen fragment-antibody sandwich complexes and the amount of said complexes is measured.

The invention relates to a longan flower extract with anti-aging, whitening, anti-allergic and cell repair functions, and the invention applies an effective dose of longan flower extract to skin cells, which has the functions of scavenging DPPH. and ABTS.+ free radicals, reducing power, chelation of iron ions and inhibiting lipid peroxide performance; inhibiting tyrosinase activity; removing nitric oxide (NO) free radicals, inhibition of lipoxygenase 1 (LOX-1) activity; inhibiting hyaluronic acid capacity, increase skin hyaluronic acid content, promoting wound repair, and reducing wrinkles production; inhibiting matrix metalloproteinases (MMPs) activity, and reducing collagen degradation; promoting cell repair ability and improving anti-allergic ability and other mechanisms.

The invention discloses a making method of anti-ageing cosmetics from ginseng and a technological process of the making method, and belongs to the technical field of preparation of cosmetics. According to the method disclosed by the invention, a ginseng extract is extracted from ginseng, the ginseng extract cooperates with various auxiliary ingredients, and the ginseng extract has the functions ofnourishing and strengthening bodies, delaying senescence, resisting radiation and the like and can relieve ageing through improving activity of antioxidase in tissue or cells of skin, strengthening the capacity for eliminating free radicals, reducing the level of lipid peroxide, restraining expression of inflammatory factors, reducing the level of substrate metalloprotease and restraining collagen degradation, so that make-up skin care products made through mixing treatment of the ginseng extract and various auxiliary materials have good efficacy of replenishing water and keeping moist, can enable the skin to be tight and elastic, can enable coarse wrinkles to be stretched, can have the efficacy of enabling fine wrinkles to be light, can enable the skin to be recovered to be compact and smooth, can have good effect of cleaning the skin, are suitable for any skin nature, do not have any side effects, and are convenient to popularize and apply.

The invention relates to an injection (intracutaneous injection) for correcting skin wrinkles and a preparation method thereof. The injection is prepared from the following components by mass: 3-6g / L of high molecular polysaccharide, 50-200mg / L of amino acid, 2-10mg / L of water-soluble vitamin and 2-5g / L of L-carnosine. A sample prepared according to the formula is capable of supplying extracellular matrix to skin and promoting synthesis of spontaneous collagen of skin. The injection has the function of a traditional filling material for supplying lost extracellular matrix, is capable of promoting synthesis of collagen and solving the problem of wrinkled and loose skin caused be senility. Furthermore, compared with a conventional filling material, the injection is lower in viscosity and is more suitable for intracutaneous injection. According to the injection prepared by the method, the problem of sodium hyaluronate and collagen degradation of skin in the aging process can be solved by means of the intracutaneous injection, and an anti-wrinkle and skin-firming effect can be achieved.

The invention relates to the technical field of collagen extraction and discloses a preparation method and a purification method of recombinant human-derived III type collagen. According to the purification method of the recombinant human-derived III type collagen, by thermal treatment of fermentation liquid, obtained by fermentation of recombinant saccharomycetes capable of giving expression to the recombinant human-derived III type collagen, at a temperature of 65-75 DEG C, degradation of the recombinant human-derived III type collagen in the fermentation liquid into small-molecule peptides is effectively prevented, endotoxins in the fermentation liquid can be reduced, and accordingly purity of the recombinant human-derived III type collagen is greatly improved to meet requirements of cosmetic raw materials, medical instruments and medical high-purity collagen. The recombinant human-derived III type collagen prepared according to the method is high in purity and low in endotoxin content and meets requirements of cosmetic raw materials, medical instruments and medical high-purity collagen.

The invention discloses a production process for inhibiting degradation of recombinant human collagen by supplementing competitive protein, and belongs to the technical field of bioengineering fermentation. The production process specifically comprises the following steps of: supplementing the competitive protein into fermentation liquor at one time to inhibit degradation of the human collagen in an induction stage of pichia pastoris fermentation; and meanwhile, after fermentation is ended, heating to inactivate enzyme activity of the fermentation liquor. The production process disclosed by the invention can guarantee stability of the collagen in a purifying process, effectively improves yield and purity, is stable and easy to realize, and is suitable for large-scale fermentation production.

The invention discloses a biomarker of the second subtype of osteoarthritis and the purpose. The purpose of biological substance in the metabolic pathway of collagen degradation and / or collagen fibrillation in the diagnosis of osteoarthritis is mainly involved.

The invention relates to a strain for producing high-efficiency collagenase and an application thereof. The strain is preserved in China Center for Type Culture Collection on October 9th, 2020, the preservation number is CCTCC M2020574, and the address is Wuhan University, Wuhan, China. The strain has collagen degradation capacity, and the produced collagenase can be applied to collagen degradation and collagen oligopeptide production. In particular, the strain has high degradation capability on collagen raw materials such as cattle bones, fish skin and the like and can be used for further enzymolysis treatment on meat processing and fishery processing byproducts such as the cattle bones and the fish skin, cod skin is adopted as a raw material, a peptide fragment with the molecular weightsmaller than 3000Da of the prepared collagen oligopeptide is more than 95%, the molecular weight is small, the uniformity is high, and the collagen is easy to absorb and utilize by a human body, effectively improves the added value of the collagen byproduct, and has good economic benefit.

Peptides synthesized to match the human α1 (I) and α2 (I) amino-telopeptide sequences of type I collagen degradation products in body fluids, preferably either Asp-GIu-Lys-Ser-Thr-Gly-Gly (SEQ ID NO:5) or Gln-Tyr-Asp-Gly-Lys-Gly-Val-Gly (SEQ ID NO:6). used as calibrators and antigens in immunoassays for detecting type I collagen degradation products in body fluids.

Extracts of plants of the Ericacea family are administered to individuals to combat the signs of aging of the skin, e.g., wrinkles and fine lines, for example by inhibiting the expression of metalloproteases, by inhibiting collagen degradation, or by inhibiting UV-induced skin degradation.