93 results about "Collagen degradation" patented technology

The invention belongs to the technical field of cosmetics, and discloses a composition with tightening and anti-wrinkle effects as well as a preparation method and application thereof. The composition disclosed by the invention comprises the following components: a radix pseudostellariae extract and hydrolyzed ginsenoside. The radix pseudostellariae extract in the composition achieves the effects of tightening and resisting wrinkles by promoting generation of collagen and elastin, the hydrolyzed ginsenoside mainly has the effects of resisting oxidation, inhibiting expression of matrix metalloproteinase and preventing collagen degradation, and after the radix pseudostellariae extract and the hydrolyzed ginsenoside are combined, the composition has a better effect of inhibiting the activity of the matrix metalloproteinase, so that the effect of resisting wrinkles is achieved. The traditional Chinese medicine composition has the effects of promoting the generation of collagen and elastin, and has a synergistic effect, so that the traditional Chinese medicine composition has an excellent anti-aging effect.

The present invention relates to a preparation method of a collagen filler for injection. Non-crosslinked collagen, crosslinked collagen, and biodegradable polymer microspheres are compounded and mixed in a certain proportion to achieve an immediate filling effect. The biodegradable polymer microspheres play a long-term role, and the amino acid components degraded from collagen in the early and middle stages can become nutrients that stimulate the production of collagen regeneration by polyester microspheres. In the present invention, the crosslinked collagen has a high yield and less water loss through a gradient irradiation crosslinking technique. The non-crosslinked collagen gel can provide a microenvironment for nutritional repair and a filling effect in the early stage, the crosslinked extracellular matrix can provide a repair microenvironment in the middle stage of filling, and the degradation of polyester microspheres in the later stage promotes collagen regeneration, enabling the skin to have a good microenvironment throughout the repair cycle and increasing skin elasticity.

The present invention relates to the fields of medicine and cosmetology, and discloses a cell active peptide composition that promotes the regeneration of multi-type collagen structural proteins, its preparation method, and its application in medicine and cosmetology. The composition uses raw materials such as tripeptide-10 citrulline, hexapeptide-9, hydrolyzed sodium hyaluronate, γ-aminobutyric acid, decarboxylated carnosine hydrochloride, acetyl tetrapeptide-3, acetyl tetrapeptide-11, palmitoyl dipeptide-7, and palmitoyl pentapeptide-4. The composition can promote the coordinated expression of skin type I, type III, type IV, type VII, type XVII collagen, fibronectin, elastin, and glycosaminoglycans; prevent collagen cross-linking, maintain triple helix structure, and inhibit collagen degradation. Applied to dermatology and cosmetology, it can accelerate the regeneration of structural proteins such as skin collagen, repair skin damage, reduce wrinkles such as crow's feet, tear groove lines, nasolabial folds, and under-eye lines, solve the skin aging problem caused by the loss of structural proteins such as collagen, and improve skin elasticity and firmness.

The invention provides a ginseng exosome anti-aging and anti-oxidation composition and a preparation method thereof, and relates to the field of nursing materials, the ginseng exosome anti-aging and anti-oxidation composition comprises Exo nano-vesicles, procyanidine and cationic liposome, the Exo nano-vesicles are derived from ginseng extracellular fluid, the procyanidine is derived from the cationic liposome, and the cationic liposome is derived from the procyanidine. The ginseng exosome anti-aging and anti-oxidation composition provided by the invention can effectively improve skin wrinkles, enhance skin elasticity, promote collagen synthesis, inhibit collagen degradation and inhibit elastin degradation through the combined action of the Exo nano-vesicles, Pro and cationic lipidosome; degradation of elastin can cause skin to lose resilience, formation of wrinkles is accelerated, and facial skin can be effectively improved and wrinkles can be reduced by promoting synthesis of collagen and inhibiting degradation of elastin.

The invention relates to brevibacillus parabrevis and application thereof in tissue isolation, and belongs to the technical field of biology. The brevibacillus parabrevis JN-6 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.29951, and the preservation date is March 7, 2024. The brevibacillus parabrevis JN-6 disclosed by the invention has the advantages that the preservation number is CGMCC NO.29951; the brevibacillus parabrevis provided by the invention has collagen degradation capability, and the produced collagenase can be used for adipose tissue separation and is further cultured into adipose-derived stem cells. When the produced collagenase is used for digestion and separation, the number of obtained cells can be 6.17 * 10 < 6 > / mL, the adipose-derived stem cells can be effectively differentiated, and the method has a good application prospect.

The present invention relates to a damaged skin organoid model and a drug screening method using same, and is a technology that uses a technique for producing an air-liquid interface skin organoid, in which skin cells, appendages, nerve cells, adipocytes, and the like are formed as a structure similar to actual skin, and which produces damaged skin by irradiating the skin organoid with ultraviolet rays that simulate ultraviolet radiation reaching the earth's surface. In the present invention, by irradiation with simulated solar ultraviolet rays including 94.5% of UV-A and 5.5% of UV-B, the skin barrier was damaged and collagen degradation was promoted by increasing the activity of matrix metalloproteinases, and, at the same time, the characteristics of UV-damaged skin, such as increased secretion of pro-inflammatory cytokines, were successfully mimicked. Therefore, it is determined that the present invention will be effectively used as a UV-induced damage and photoaging model, and will serve as a useful technology for screening for potential therapeutic drugs.

The invention provides a beautifying composition and application thereof in whitening, anti-inflammatory and anti-aging products, and belongs to the technical field of cosmetics. The beautifying composition is prepared from collagen peptide, sodium hyaluronate and ergothioneine, wherein the mass concentration ratio of the collagen peptide to the sodium hyaluronate to the ergothioneine is (75 to 85) to (3.5 to 10) to (0.4 to 3.5); the beautifying composition shows remarkable advantages in the aspects of collagen regeneration and skin improvement, the collagen content can be remarkably increased, and multi-effect collagen regeneration promotion is achieved by inhibiting collagen degrading enzyme MMP-1 and up-regulating TGF-beta1; the content of AQP3 and HA can be increased, and water locking and moisturizing of the skin are enhanced; by activating an Nrf2 pathway, the SOD2 level is improved, IL-6 and IL-1alpha are inhibited, and the antioxidant, anti-inflammatory and whitening effects are achieved; the thickness of an epidermal layer and the density of dermal fibroblasts can be remarkably improved, wrinkle resistance and repairing are effectively achieved, and the skin structure is improved.

The invention provides a method for improving the stability of a dentin bonding interface through phosphorylated chitosan, and belongs to the field of biological medicine. The preparation method comprises the following steps: mixing phosphorylated chitosan with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and N-hydroxysuccinimide, and uniformly coating the mixture on the surface of dentin to form a covalent cross-linked network and induce in-situ deposition of hydroxyapatite. A series of performance changes are brought to a dentin bonding interface, and the mechanical strength and the enzymolysis resistance of dentin collagen fibers are improved. The adhesive interface is endowed with the capability of inhibiting bacteria, so that the demineralized dentin collagen fibers are preserved, and the adhesive interface is stabilized. The dentin collagen fiber is stabilized, a proper remineralization microenvironment is maintained, the deposition of minerals such as calcium and phosphorus on the surface of the dentin is promoted, and meanwhile, a guarantee is provided for the dentin to resist demineralization. According to the invention, the three problems of micro leakage, collagen degradation and remineralization are simultaneously solved in the field of dentin bonding for the first time.

The invention provides an anti-aging repairing composition containing algae and application of the anti-aging repairing composition, the anti-aging repairing composition comprises blue-green algae, tripeptide and a bacillus fermentation product, and the mass ratio of the blue-green algae to the tripeptide to the bacillus fermentation product is (0.01-0.5): (0.1-1): (0.2-2). The composition obtained by compounding the blue-green algae, the tripeptide and the bacillus fermentation product has a synergistic effect in the aspect of resisting senility, especially in the aspect of promoting the secretion amount of collagen, and when the blue-green algae is replaced with other algae or the bacillus fermentation product is replaced with other fungus fermentation products, the effect of resisting senility is achieved. The effects of the composition disclosed by the invention cannot be achieved. Meanwhile, the composition provided by the invention also has multiple effects of repairing a barrier, resisting oxidation, resisting inflammation, promoting intercellular interaction, promoting collagen synthesis, inhibiting collagen degradation and the like.

The invention aims to solve the problems that the existing skin photoaging evaluation technology can only realize static detection, lacks environment dynamic monitoring and cannot capture day and night changes of physiological parameters. According to the system, key parameters such as skin percutaneous moisture loss, tissue impedance and temperature are continuously measured through a wearable flexible electronic patch, a portable environment monitoring module is used for measuring UVA / UVB intensity and pollutants and is linked with satellite meteorological data to construct a personal exposure map, and a non-invasive microneedle sensing technology is used for detecting skin interstitial fluid biomarkers. A time-space diagram neural network model is used for fusing data, oxidative stress, collagen degradation and environmental toxicity are comprehensively considered, a light aging index is output, clinical intervention suggestions are provided through a data analysis and prediction module, and dynamic accurate evaluation is achieved.

The invention provides an anti-aging and skin-tightening composition and a preparation method thereof, and belongs to the technical field of anti-aging and skincare, and the composition comprises a hovenia acerba infructescence extract, an okra seed extract, tetrapeptide-4, acetyl octapeptide-1, a hill-pit snail extract, paeonol and a carnosine-zinc gluconate chelate. And carrying out aerobic fermentation on the raisin tree infructescence powder through aspergillus oryzae, and purifying the centrifuged supernatant through a composite filler resin column to obtain the raisin tree infructescence extract. The preparation method comprises the following steps: sequentially carrying out enzymolysis on okra seeds by virtue of compound enzyme, ficin and subtilisin, adding absolute ethyl alcohol for extraction, centrifuging supernate, and purifying by virtue of an LH-20 sephadex column, so as to obtain the okra seed extract. The preparation method comprises the following steps: sequentially carrying out enzymolysis on the mountain snail meat through trypsin and lumbrukinase, centrifuging the enzymatic hydrolysate, and carrying out ultrafiltration on the supernate to obtain the mountain snail extract with the molecular weight of less than 3kDa. The components are matched for use, so that the balance of high-efficiency anti-aging and low-irritation can be realized through synergism of three major pathways of inhibiting collagen degradation, promoting collagen synthesis and resisting sugar and relieving.

The invention relates to the technical field of collagen intelligent monitoring, and particularly discloses an intelligent collagen monitoring method and system integrated with a microsensor. Enzyme response hydrogel with switchable states is anchored to the skin through a microneedle array, a high-frequency acoustic impedance analysis module is used for continuously probing the acoustic impedance change of the hydrogel, and the collagen monitoring accuracy is improved. Identifying a collagen degradation event; once collagen degradation is detected, the system activates the micro-regeneration unit, a cleaning solution and a regeneration solution are conveyed through a microfluidic channel, a broken peptide chain is removed, a hydrogel cross-linked network is reconstructed, and functional regeneration of the sensing unit is realized; the system further comprises a regeneration state confirmation and cycle reset mechanism, and the reliability of each monitoring cycle and the consistency of data references are ensured. According to the method, the limitation of a traditional monitoring method is overcome, multiple times of recycling of a single sensor is achieved, the credibility and consistency of long-term monitoring data are improved, and the method has important significance on skin health management and senescence research.

The invention belongs to the technical field of biological medicines, and particularly relates to a method for detecting the degradation capacity of human macrophages to liver collagen in vitro. According to the scheme provided by the invention, the content of CTX I, CTX III, C3M or C1M of the liver type I collagen degradation fragment in the macrophage supernatant is detected through Elisa, the degradation capability of the macrophage on the liver collagen can be accurately reflected, and the macrophage with the liver collagen degradation capability is qualified when the CTX I of the macrophage culture supernatant is greater than 100ng / mL.

The present invention relates to a cosmetic composition for improving skin elasticity or reducing pore size, comprising an amphiphilic biocompatible polymer. The composition promotes collagen production, inhibits collagen degradation, and suppresses elastin degradation, and thus can be effectively used as a cosmetic composition for reducing the number of pores and decreasing pore areas.

The application belongs to the technical field of biological medicine, and relates to application of a CXCL14 inhibitor in preparation of a drug for preventing or treating abdominal aortic aneurysm of a patient. The inhibitor effectively eliminates the gender difference of abdominal aortic aneurysm by specifically blocking the combination of CXCL14 and its receptor CXCR4, significantly reduces aortic macrophage infiltration and PDGFRA + macrophage accumulation in the progress of abdominal aortic aneurysm, reduces the degree of collagen degradation and elastic fiber damage, and thus inhibits the pathological progress of abdominal aortic aneurysm. The application provides a new target and drug combination for precise treatment of male abdominal aortic aneurysm, and has clear clinical application value.

This invention provides a magnesium baicalein hydrogel with whitening and anti-skin aging effects and its preparation method. The formulation includes magnesium baicalein, sodium hyaluronate, carbomer, propylene glycol, niacinamide, a pH adjuster, and purified water. The hydrogel uses magnesium baicalein as the main active ingredient, overcoming the traditional deficiency of baicalein's poor water solubility and exhibiting good gel phase stability. The magnesium baicalein in the hydrogel can effectively scavenge free radicals and prevent collagen degradation to combat aging; simultaneously, it inhibits tyrosinase, synergistically achieving a dual whitening effect with niacinamide.

The invention discloses a peptide with an anti-photoaging effect as well as a preparation method and application of the peptide. The cyclic dipeptide cyclo (LO) and the linear dipeptide LO disclosed by the invention can be used for remarkably inhibiting the activity of MMP-1 and efficiently blocking collagen degradation. In addition, the cyclic dipeptide and the linear dipeptide have no cytotoxic effect when being co-cultured with fibroblasts, so that the cyclic dipeptide and the linear dipeptide have no stimulation and side effects on cells, and are high in safety and good in stability. The cyclic dipeptide and the linear dipeptide disclosed by the invention can resist UVA (Ultraviolet A) damage, have a promoting effect on cell proliferation activity after UVA irradiation and have an anti-aging effect on cells after UVA irradiation. The two peptides disclosed by the invention can be used for preparing medicines or cosmetics for resisting light aging, resisting aging, beautifying and protecting skin. The peptide fragment disclosed by the invention has a good application prospect in the fields of medicines, cosmetics and the like.

The invention belongs to the technical field of cancer treatment, and discloses a method for inhibiting castration-resistant prostate cancer proliferation and metastasis by promoting collagen degradation through targeting ADAMTS1. According to the method, ADAMTS1 is determined to be a mechanism key point for linking epigenetic reprogramming with biomechanical microenvironment destruction. The ADAMTS1 is reactivated to degrade a collagen-rich matrix, the FAK / MAPK mechanical transduction signaling pathway is inhibited, and the epithelial-mesenchymal transition (EMT) is reversed. The strategy not only achieves more than 90% of tumor inhibition effect, but also exhausts immunosuppressive macrophages and regulatory T cells to reverse immunosuppression by enhancing toxic CD8 + T cell infiltration. The research determines that epigenetic-ECM coevolution is a symbolic feature of CRPC, and provides a reasonably designed combination therapy for treating castration-resistant prostate cancer.

An instant fish maw processing technology for delaying degradation of collagen belongs to the technical field of instant fish maw processing, and comprises the following steps: S1, treating fish maw with 0.1% of tea polyphenol, 0.05% of a rosemary extract and 0.2% of chitosan oligosaccharide for 30 min, carrying out enzyme inhibition treatment, and then cleaning and draining for later use; s2, dipping the fish gelatin treated in the step S1 in a solution containing 0.1% of riboflavin and 0.2% of hyaluronic acid, irradiating the fish gelatin with white light for 10s, and then cleaning and draining the fish gelatin for later use; and S3, putting 5-7 parts by weight of fish maw and 65-80 parts by weight of pure milk into a container, sealing, and sterilizing to obtain the instant fish maw. Through enzyme inhibition treatment, the hydrolysis capability of metalloproteinase on collagen is reduced or eliminated, and degradation of the collagen is reduced, so that the nutritional value of the fish maw is maintained; collagen molecules in the fish maw and riboflavin are subjected to a cross-linking reaction to form a stable three-dimensional network structure, so that the acting force between the collagen molecules is enhanced, the structural stability of the collagen is improved, and the degradation of the collagen is delayed.

The invention provides a method for improving the purity of collagen in the fermentation process of engineering bacteria and application of the method, and relates to the technical field of fermentation. Dithiothreitol (DTT) is used as an exogenous additive for the first time to be applied to the process of producing collagen through strain fermentation, and it is found that DTT can relieve intracellular stress of yeast cells and effectively relieve the collagen degradation condition in the fermentation process, and a fermentation product with higher purity is obtained. The invention provides a novel method for producing collagen through fermentation of engineering bacteria, and the method is simple and easy to operate, can effectively improve the purity of a fermentation product, and has a wide application prospect.

This invention relates to an anti-aging and repair composition containing hyaluronic acid peptides and its application. The composition comprises the following components: hyaluronic acid peptide composition, *Ulmus pumila* root extract, *Comfrey* extract, and *Alfalfa* extract; wherein the mass ratio of the hyaluronic acid peptide composition, *Ulmus pumila* root extract, *Comfrey* extract, and *Alfalfa* extract is (0.05-5):(0.1-3):(0.1-2):(0.1-2); the hyaluronic acid peptide composition includes yeast extract, collagen, and tetradecylaminobutyryl valine butyrate trifluoroacetate. The composition of this invention has a dual anti-aging effect of promoting collagen synthesis and inhibiting collagen degradation, effectively improving signs of aging such as skin laxity and fine lines. It also achieves a full-chain repair process of soothing, repairing, and strengthening, alleviating sensitivity and discomfort, strengthening the skin barrier, and ultimately achieving a highly effective and gentle anti-aging and repair effect.

The invention belongs to the technical field of cosmetics, and particularly relates to a composition for promoting collagen regeneration and inhibiting expression lines for sensitive skin and application of the composition. The invention provides a composition for promoting collagen regeneration and inhibiting expression lines for sensitive skin, the composition is prepared by compounding an Agrocarpus caffeus seed extract, a selaginella tamariscina extract, a lithospermum altaicum extract and acetyl hexapeptide-1, and the four components cooperate through a signal at a pharmacodynamic level, so that the composition has the effects of promoting collagen regeneration and inhibiting expression lines. A complete regulation and control network from inhibition of collagen degradation, promotion of synthesis to barrier repair is formed, the multi-target synergistic effect efficiency is remarkably improved, and full-path regulation and control from inhibition of facial wrinkles and promotion of collagen regeneration to barrier repair are realized; the defects that in the prior art, an action mechanism is single, and a long-term and systematic repairing effect is lacked are effectively overcome, and meanwhile, the mildness and sensitive skin applicability of the formula are remarkably improved.

The invention discloses application of cyclic peptide or salt thereof in cosmetics, and belongs to the technical field of cosmetic peptides, the natural cyclic peptide comprises alanine (Ala), glycine (Gly), proline (Pro), valine (Val) and tyrosine (Tyr), and is a compound with the following formula I. The invention further discloses a preparation method of the natural cyclic peptide. The cyclopeptide compound disclosed by the invention has the effects of promoting generation of type I collagen and elastin, inhibiting generation of matrix metalloproteinase I (MMP-1) under UVA irradiation and preventing collagen degradation, so that the cyclopeptide compound has the effects of resisting wrinkles, tightening skin and the like.

The invention belongs to the technical field of biological fermentation, and particularly relates to a fermentation method of a recombinant A-type humanized III-type collagen strain. According to the fermentation method, degradation of collagen can be reduced, and meanwhile the fermentation yield can be increased. According to the method, ammonium citrate and bovine bone peptone are added at one time in the fermentation process, and then methanol fed-batch induced expression is carried out, so that the purpose of reducing collagen degradation is achieved. Results of the embodiment of the invention show that the fermentation method provided by the invention can improve the biosynthesis rate of the recombinant human collagen, increase the expression quantity of the recombinant human collagen and reduce the degradation rate of the collagen, and the fermentation yield level is improved by 10-20% or above compared with that of a common fermentation mode.

The invention discloses a recombinant humanized collagen gel preparation and a preparation method and application thereof, and relates to the technical field of daily chemicals. The invention uses grape seed extract and silanized hyaluronic acid ester (SHAC) in combination on the basis of recombinant humanized collagen, which can supplement collagen fibers in the skin, promote the proliferation of human skin keratinocytes and fibroblasts and collagen synthesis, inhibit collagen degradation, repair the skin, enhance the skin barrier and moisturize; inhibit the cross-linking effect of collagenase and elastase on connective tissue, improve skin immunity, and maintain skin elasticity; and has good antioxidant properties, can neutralize free radicals, effectively prevent skin aging, thereby obtaining excellent moisturizing and anti-wrinkle properties. The recombinant humanized collagen gel preparation of the invention can promote fibroblast proliferation, has excellent moisturizing properties, and can significantly improve skin elasticity, and can be used in various moisturizing and anti-wrinkle skin care products.

The Inventors have developed an ELISA of a new molecular marker detecting a neoepitope generated from the cleavage of the α1 chain of type III collagen within its helical domain. Serum levels of this marker were significantly increased in patients with RA and is significantly associated to CRP and ESR levels. Indeed, they demonstrated that the median serum HELIX-III levels were significantly higher in patients with moderate (p=0027) and active RA (p=00004) compared with those in age-matched controls. The present invention relates to an antibody recognizing an epitope having SEQ ID NO:1 of collagen protein and its uses for diagnostic, prognostic and monitoring purposes.

The invention discloses an anti-aging composition containing recombinant collagen. The composition comprises the following components: (1) 2-5g of recombinant collagen; (2) 1-2 g of hyaluronic acid; (3) 0.2 to 0.5 g of a collagen degradation product; (4) 0.2 to 0.3 g of an anti-TGF (Transforming Growth Factor)-beta 1 monoclonal antibody; (5) 0.2 to 0.5 g of vitamin C; (6) 0.2 to 0.3 g of vitamin E; (7) 0.5-1 g of a green tea extract; and (8) adding the aqueous solution to 100 g. The anti-aging composition can effectively delay the skin aging process, improve the skin elasticity and reduce generation of wrinkles and fine wrinkles. The composition not only can be used in daily skin care products, but also can be used for treating skin problems caused by aging, such as skin laxity, fine wrinkles and darkness, and has a remarkable market prospect.

The invention relates to the field of medicine and cosmetology, and discloses a cell active peptide composition for promoting regeneration of multi-type collagen structure protein, a preparation method of the cell active peptide composition and application of the cell active peptide composition in medicine and cosmetology. The composition is prepared from the following raw materials: tripeptide-10 citrulline, hexapeptide-9, hydrolyzed sodium hyaluronate, gamma-aminobutyric acid, decarboxylated carnosine hydrochloride, acetyl tetrapeptide-3, acetyl tetrapeptide-11, palmitoyl dipeptide-7, palmitoyl pentapeptide-4 and the like. The composition can promote the synergistic expression of I-type, III-type, IV-type, VII-type and XVII-type collagen, fibronectin, elastin and glycosaminoglycan of the skin; collagen crosslinking is prevented, a triple-helix structure is maintained, and collagen degradation is inhibited. When being applied to skin medicine and beauty treatment, the composition can accelerate regeneration of structural proteins such as skin collagen, repair skin injury, reduce wrinkles such as crow's feet, lacrimal ducts, nasolabial folds and subocular wrinkles, solve the problem of skin aging caused by loss of the structural proteins such as collagen, and improve skin elasticity and compactness.

The present application relates to the technical field of biological medicine materials, and particularly relates to a collagen-based repair membrane material for oral cavity and a preparation method thereof. Specifically, the present application comprises the following components: collagen type I, bioactive glass nanoparticles, gamma-aminopropyl triethoxysilane, a crosslinking system, polyaspartic acid, CaCl2, K2HPO4 and Sr-Zn solution. In the present application, polyaspartic acid is used to guide in-situ mineralization of calcium and phosphorus to form an oriented compatible nanocrystalline template, chemical coupling and continuous ion release of silanized bioactive glass are combined to realize time sequence cooperation of mineral deposition and ion supply; a unidirectional freezing structure is used to build a structure gradient with a dense cavity surface and a porous bone side, so as to give the structure the functions of anti-permeation and cell guidance; mild crosslinking containing alcohol is combined to improve the wet strength and control swelling; further, the bone side is activated by Sr-Zn solution to form a directional microenvironment, so as to promote interface mineralization maturity and delay collagen degradation, and realize the oral cavity repair function of multi-module cooperation.

The invention discloses a compound antifreeze agent and a preparation method thereof as well as a freezing method of meat products, and belongs to the technical field of refrigeration refrigerants. Collagen peptide with a freeze-thaw protection effect is adopted, and glutathione and sodium erythorbate are compounded, so that the freeze-thaw protection effect is improved; the prepared safe and green compound antifreeze agent is applied to freezing and cold storage of meat products, the generation of ice crystals and the size of the ice crystals are reduced in the freezing process, and the activity of metalloproteinase is inhibited in the melting process of the meat products, so that degradation of collagen is reduced, cell rupture and liquid loss are reduced, and the quality of the meat products is improved. The texture, weight and color of the product are furthest maintained, and the eating quality is improved.