32 results about "Collagen degradation" patented technology

The invention aims to solve the problems that the existing skin photoaging evaluation technology can only realize static detection, lacks environment dynamic monitoring and cannot capture day and night changes of physiological parameters. According to the system, key parameters such as skin percutaneous moisture loss, tissue impedance and temperature are continuously measured through a wearable flexible electronic patch, a portable environment monitoring module is used for measuring UVA / UVB intensity and pollutants and is linked with satellite meteorological data to construct a personal exposure map, and a non-invasive microneedle sensing technology is used for detecting skin interstitial fluid biomarkers. A time-space diagram neural network model is used for fusing data, oxidative stress, collagen degradation and environmental toxicity are comprehensively considered, a light aging index is output, clinical intervention suggestions are provided through a data analysis and prediction module, and dynamic accurate evaluation is achieved.

The invention relates to the technical field of collagen intelligent monitoring, and particularly discloses an intelligent collagen monitoring method and system integrated with a microsensor. Enzyme response hydrogel with switchable states is anchored to the skin through a microneedle array, a high-frequency acoustic impedance analysis module is used for continuously probing the acoustic impedance change of the hydrogel, and the collagen monitoring accuracy is improved. Identifying a collagen degradation event; once collagen degradation is detected, the system activates the micro-regeneration unit, a cleaning solution and a regeneration solution are conveyed through a microfluidic channel, a broken peptide chain is removed, a hydrogel cross-linked network is reconstructed, and functional regeneration of the sensing unit is realized; the system further comprises a regeneration state confirmation and cycle reset mechanism, and the reliability of each monitoring cycle and the consistency of data references are ensured. According to the method, the limitation of a traditional monitoring method is overcome, multiple times of recycling of a single sensor is achieved, the credibility and consistency of long-term monitoring data are improved, and the method has important significance on skin health management and senescence research.

The invention belongs to the technical field of biological medicines, and particularly relates to a method for detecting the degradation capacity of human macrophages to liver collagen in vitro. According to the scheme provided by the invention, the content of CTX I, CTX III, C3M or C1M of the liver type I collagen degradation fragment in the macrophage supernatant is detected through Elisa, the degradation capability of the macrophage on the liver collagen can be accurately reflected, and the macrophage with the liver collagen degradation capability is qualified when the CTX I of the macrophage culture supernatant is greater than 100ng / mL.

The application belongs to the technical field of biological medicine, and relates to application of a CXCL14 inhibitor in preparation of a drug for preventing or treating abdominal aortic aneurysm of a patient. The inhibitor effectively eliminates the gender difference of abdominal aortic aneurysm by specifically blocking the combination of CXCL14 and its receptor CXCR4, significantly reduces aortic macrophage infiltration and PDGFRA + macrophage accumulation in the progress of abdominal aortic aneurysm, reduces the degree of collagen degradation and elastic fiber damage, and thus inhibits the pathological progress of abdominal aortic aneurysm. The application provides a new target and drug combination for precise treatment of male abdominal aortic aneurysm, and has clear clinical application value.

This invention provides a magnesium baicalein hydrogel with whitening and anti-skin aging effects and its preparation method. The formulation includes magnesium baicalein, sodium hyaluronate, carbomer, propylene glycol, niacinamide, a pH adjuster, and purified water. The hydrogel uses magnesium baicalein as the main active ingredient, overcoming the traditional deficiency of baicalein's poor water solubility and exhibiting good gel phase stability. The magnesium baicalein in the hydrogel can effectively scavenge free radicals and prevent collagen degradation to combat aging; simultaneously, it inhibits tyrosinase, synergistically achieving a dual whitening effect with niacinamide.

The invention discloses a peptide with an anti-photoaging effect as well as a preparation method and application of the peptide. The cyclic dipeptide cyclo (LO) and the linear dipeptide LO disclosed by the invention can be used for remarkably inhibiting the activity of MMP-1 and efficiently blocking collagen degradation. In addition, the cyclic dipeptide and the linear dipeptide have no cytotoxic effect when being co-cultured with fibroblasts, so that the cyclic dipeptide and the linear dipeptide have no stimulation and side effects on cells, and are high in safety and good in stability. The cyclic dipeptide and the linear dipeptide disclosed by the invention can resist UVA (Ultraviolet A) damage, have a promoting effect on cell proliferation activity after UVA irradiation and have an anti-aging effect on cells after UVA irradiation. The two peptides disclosed by the invention can be used for preparing medicines or cosmetics for resisting light aging, resisting aging, beautifying and protecting skin. The peptide fragment disclosed by the invention has a good application prospect in the fields of medicines, cosmetics and the like.

The invention belongs to the technical field of cancer treatment, and discloses a method for inhibiting castration-resistant prostate cancer proliferation and metastasis by promoting collagen degradation through targeting ADAMTS1. According to the method, ADAMTS1 is determined to be a mechanism key point for linking epigenetic reprogramming with biomechanical microenvironment destruction. The ADAMTS1 is reactivated to degrade a collagen-rich matrix, the FAK / MAPK mechanical transduction signaling pathway is inhibited, and the epithelial-mesenchymal transition (EMT) is reversed. The strategy not only achieves more than 90% of tumor inhibition effect, but also exhausts immunosuppressive macrophages and regulatory T cells to reverse immunosuppression by enhancing toxic CD8 + T cell infiltration. The research determines that epigenetic-ECM coevolution is a symbolic feature of CRPC, and provides a reasonably designed combination therapy for treating castration-resistant prostate cancer.

The invention provides a method for improving the purity of collagen in the fermentation process of engineering bacteria and application of the method, and relates to the technical field of fermentation. Dithiothreitol (DTT) is used as an exogenous additive for the first time to be applied to the process of producing collagen through strain fermentation, and it is found that DTT can relieve intracellular stress of yeast cells and effectively relieve the collagen degradation condition in the fermentation process, and a fermentation product with higher purity is obtained. The invention provides a novel method for producing collagen through fermentation of engineering bacteria, and the method is simple and easy to operate, can effectively improve the purity of a fermentation product, and has a wide application prospect.

This invention relates to an anti-aging and repair composition containing hyaluronic acid peptides and its application. The composition comprises the following components: hyaluronic acid peptide composition, *Ulmus pumila* root extract, *Comfrey* extract, and *Alfalfa* extract; wherein the mass ratio of the hyaluronic acid peptide composition, *Ulmus pumila* root extract, *Comfrey* extract, and *Alfalfa* extract is (0.05-5):(0.1-3):(0.1-2):(0.1-2); the hyaluronic acid peptide composition includes yeast extract, collagen, and tetradecylaminobutyryl valine butyrate trifluoroacetate. The composition of this invention has a dual anti-aging effect of promoting collagen synthesis and inhibiting collagen degradation, effectively improving signs of aging such as skin laxity and fine lines. It also achieves a full-chain repair process of soothing, repairing, and strengthening, alleviating sensitivity and discomfort, strengthening the skin barrier, and ultimately achieving a highly effective and gentle anti-aging and repair effect.

The invention belongs to the technical field of cosmetics, and particularly relates to a composition for promoting collagen regeneration and inhibiting expression lines for sensitive skin and application of the composition. The invention provides a composition for promoting collagen regeneration and inhibiting expression lines for sensitive skin, the composition is prepared by compounding an Agrocarpus caffeus seed extract, a selaginella tamariscina extract, a lithospermum altaicum extract and acetyl hexapeptide-1, and the four components cooperate through a signal at a pharmacodynamic level, so that the composition has the effects of promoting collagen regeneration and inhibiting expression lines. A complete regulation and control network from inhibition of collagen degradation, promotion of synthesis to barrier repair is formed, the multi-target synergistic effect efficiency is remarkably improved, and full-path regulation and control from inhibition of facial wrinkles and promotion of collagen regeneration to barrier repair are realized; the defects that in the prior art, an action mechanism is single, and a long-term and systematic repairing effect is lacked are effectively overcome, and meanwhile, the mildness and sensitive skin applicability of the formula are remarkably improved.

To provide a new technology of estimating a hair cycle.SOLUTION: The present invention is a hair cycle estimation method for estimating a hair cycle of hair follicles in an evaluation target based on analysis results of collagen degradation and / or neogenesis in perifollicular tissues including hair follicles.SELECTED DRAWING: None

The application discloses a pichia pastoris bioengineering bacterium for relieving degradation of recombinant collagen, a preparation method and application thereof. Through bioinformatics analysis, gene knockout and function verification screening, three protease genes capable of relieving degradation of collagen in a pichia pastoris host are obtained, namely, chr1-4_0611, chr1-4_0362 and chr2-2_0380. Knocking out the protease chr1-4_0611 can make the total protein yield accumulation increase to 3.5 times of that of a control bacterium, and the proportion of a target band is greater than 50%, which can provide good theoretical and technical guidance for effective expression of other recombinant proteins in the pichia pastoris host.

The invention relates to the technical field of biological medicine, in particular to a collagen gel delivery system integrated with polypeptide medicine and a preparation method and application thereof.The collagen gel delivery system is characterized in that collagen serves as a basic gel matrix, covalent cross-linking is conducted through 4-arm-PEG-NHS, then active polypeptide is connected into a gel network through amido bonds, and the collagen gel delivery system integrated with the polypeptide medicine is obtained. The active polypeptide is an antibacterial peptide and / or an MMP-9 cyclopeptide inhibitor. The collagen gel delivery system is expected to realize the efficient and ordered healing promotion effect on the wound infected by diabetes mellitus through a multi-target synergistic mechanism such as anti-infection, anti-inflammation, collagen degradation inhibition and collagen synthesis promotion, and provides a new idea and a new option for clinical response.

The invention discloses a mesenchymal stem cell compound preparation for treating cartilage injury, and belongs to the technical field of cell biology. The composite preparation consists of human umbilical cord mesenchymal stem cells (hUC-MSC) subjected to specific pretreatment and a pharmaceutical carrier. The pretreatment method sequentially comprises the following steps: carrying out low-oxygen pretreatment on the hUC-MSC; then, carrying out pretreatment on a ferroptosis inhibitor Ferrostatin-1 under a low-oxygen condition; and finally, carrying out icariin pretreatment under a normal oxygen condition. The unique triple pretreatment strategy generates a remarkable synergistic effect, and can greatly improve the survival rate and the function maintenance capability of the hUC-MSC in the cartilage injury inflammatory microenvironment. Experimental results prove that the preparation can significantly inhibit collagen degradation and promote cartilage matrix regeneration, and the treatment efficiency of stem cell transplantation and the repair effect of cartilage injury are effectively improved.

The invention relates to the technical field of biological medicines, and discloses a pharmaceutical composition, a preparation method and application thereof, and a medicine for promoting wound healing. The pharmaceutical composition comprises a cathepsin K inhibitor and an exosome. Through combined use of the cathepsin K inhibitor and the exosome, especially the exosome derived from mesenchymal stem cells, the defects of an existing diabetes wound treatment method are overcome, collagen degradation is reduced, and collagen deposition is promoted; angiogenesis is enhanced, and wound blood supply is improved; the cell survival rate is improved; energy metabolism is recovered, and cell functions are enhanced. Moreover, the purposes of repairing and healing wounds more efficiently can be achieved on the premise that the dosage is smaller, the production cost is reduced, and the treatment feasibility and clinical popularization value are improved.

The objective of the present invention is to provide a food-derived collagen production promoter and collagen degradation inhibitor that is effective and safe, and can be consumed on a daily basis. [Solution] The present invention provides a collagen production promoter and a collagen degradation inhibitor characterized by containing Bacopa monnieri, and a collagen production promoter and a collagen degradation inhibitor characterized by containing Bacopa monnieri and Vitis coignetiae. The collagen production promoter and collagen degradation inhibitor of the present invention exhibit excellent collagen production promoting and collagen degradation inhibiting effects, and can therefore be used as a food, quasi-drug, or pharmaceutical product that has preventive and ameliorative effects on various symptoms and diseases related to the decrease of collagen in the body.

The invention discloses an anti-wrinkle and skin-brightening composition containing hydrolyzed ginsenosides and ergothioneine as well as a preparation method and application of the anti-wrinkle and skin-brightening composition, and belongs to the technical field of cosmetics. The composition is prepared from the following components in parts by weight: 5 to 15 parts of ergothioneine, 25 to 40 parts of sphingomonas fermentation product extract, 15 to 30 parts of hydrolyzed ginsenosides and 30 to 40 parts of iris vetiveria root extract. Wherein the hydrolyzed ginsenosides are prepared by enzymolysis of a specific compound enzyme. The four components in the composition synergistically construct an anti-oxidation-anti-inflammation-collagen promotion-collagen degradation inhibition system, the composition has excellent anti-wrinkle, skin brightening, soothing and anti-oxidation effects, and the composition is good in safety, suitable for various skin types, capable of being prepared into essence, emulsion and other cosmetics according to the dosage of 0.01 wt%-30 wt% and wide in application prospect.

This invention relates to the field of biotechnology and discloses a stem cell co-expressing miR-449a-5p and miR-30c-5p. The stem cell is constructed by cloning a biomimetic polycistronic expression cassette containing miR-449a-5p and miR-30c-5p into a lentiviral vector and infecting the stem cell, enabling stable and synergistic expression of these two miRNAs. The exosomes (miRNAs-Exos) secreted by these cells specifically enrich miR-449a-5p and miR-30c-5p; after local administration, the exosomes are taken up by skin cells, and the two miRNAs synergistically inhibit the expression of the aging-associated secretory phenotype (SASP) core factors CCL2 and PAI-1, downregulate matrix metalloproteinases MMP-1 / MMP-3, reduce collagen degradation, improve the dermal inflammatory microenvironment, and thereby promote collagen synthesis and skin barrier repair.

An assay measuring protease mediated degradation of type IV collagen and its biomarker potential for identifying cancer patients with a T-cell permissive tumor microenvironment is described.

This invention belongs to the field of cosmetic technology, specifically relating to an anti-aging composition containing nanospheres and its preparation method, as well as its application in skincare products. The method employs a double emulsion solvent evaporation method to prepare nanospheres. Soybean lecithin and polylactic acid-glycolic acid copolymer are dissolved in an organic solvent as the oil phase. Superoxide dismutase, collagenase, trehalose, and bovine serum albumin are dissolved in phosphate buffer as the inner aqueous phase. After emulsification to form a primary emulsion, it is added to an outer aqueous phase containing polyvinyl alcohol and homogenized to form a double emulsion. After solidification, centrifugation, and washing, the nanospheres are obtained. The resulting composition exhibits good enzyme protection and skin permeability, effectively scavenging free radicals and inhibiting collagen degradation, showing broad application prospects in the skincare field. This invention features a simple process, uses commercially available raw materials, and is suitable for large-scale production.

Described herein is an assay for measuring protease-mediated degradation of type IV collagen and its biomarker potential for identifying cancer patients with a T-cell permissive tumor microenvironment.

The invention relates to the technical field of daily chemicals, and discloses a skin anti-aging composition as well as a preparation method and application thereof. The invention provides a skin anti-aging composition and a preparation method thereof. Comprising the following raw materials in parts by weight: 1 to 20 parts of hydroxypropyl tetrahydropyrantriol, 1 to 10 parts of vaccinium myrtillus fruit extract, 0.1 to 5 parts of tetrahydromethylpyrimidine carboxylic acid, 0.5 to 1.5 parts of paeonia lactiflora root extract, 0.01 to 5 parts of leontopodium alpinum callus extract, 0.01 to 5 parts of crithmum maritimum callus culture filtrate, 0.001 to 4 parts of ergothioneine and 0.1 to 20 parts of animal umbilical cord extract. According to the composition, various extracts and active ingredients are compounded, so that the composition has a synergistic effect, can directly reach the corium layer of skin, improves cell energy metabolism, improves mitochondrial functions, effectively removes free radicals of mitochondria, improves collagen degradation, and improves skin aging through a dual-channel of mitochondrial activation and extracellular matrix repair.

The application discloses injectable long-acting collagen and a preparation method and application thereof, and belongs to the technical field of collagen preparation. The application solves the problems of rapid degradation and insufficient mechanical property of collagen after injection into the body, and the problem of inability to have biological safety, good biological activity and injectability. The long-acting collagen is prepared by taking methoxypolyethylene glycol active ester and collagen as raw materials. Amide reaction is caused between the methoxypolyethylene glycol active ester and lysine residues on the collagen, hydrophilic PEG is introduced on the surface of the collagen, a hydration layer is formed, biological enzymes are hindered from contacting the collagen, collagen degradation is delayed, and the single-arm structure of the methoxypolyethylene glycol active ester can avoid product agglomeration and abnormal viscosity caused by excessive cross-linking. The long-acting collagen has a long retention time in the body, has no obvious cytotoxicity, has high biological safety, and maintains cell proliferation, migration and adhesion activity and biological repair functions.

The invention relates to an anti-aging composition containing silkworm extract as well as a preparation method and application of the anti-aging composition, and belongs to the technical field of cosmetics. The anti-aging composition comprises hydroxypropyl cyclodextrin, lipidosome and anti-aging active ingredients loaded in the lipidosome, the anti-aging active ingredients comprise ergothioneine, resveratrol and silkworm extract, and the ergothioneine, the resveratrol and the silkworm extract have a synergistic effect, so that the resistance of the anti-aging composition to oxidative stress can be enhanced, collagen degradation can be inhibited, and cell damage can be reduced. Furthermore, the liposome prepared from phosphatidylcholine, polyquaternium-61 and oilseed rape sterol is used for loading the anti-aging active component, so that the stability and transdermal performance of the anti-aging active component are improved, the liposome breaks through a cuticle barrier, the active component is effectively conveyed to the deep layer of skin, the bioavailability is improved, and the bioavailability is improved. Further, the effects of long-acting oxidation resistance, senescence delaying and the like are achieved.

The present application relates to the field of cosmetics, in particular to oligopeptides and application thereof. The present application provides oligopeptides, the amino acid sequence of which is shown in SEQ ID NO:1 or SEQ ID NO:2. The present application is based on a series of oligopeptides (including beta-6 and beta-683) designed based on the key role interface of beta-Klotho protein, and the calculation model predicts that the oligopeptides have good binding potential and the characteristics of maintaining activity after truncation; the series of oligopeptides act on the FGF21-FGFR signaling pathway by simulating the function of beta-Klotho, thereby promoting collagen synthesis, inhibiting collagen degradation, and ultimately achieving the beneficial effect of skin anti-aging.

The invention belongs to the technical field of biological medicines, and relates to application of a CXCL14 inhibitor in preparation of a medicine for preventing or treating abdominal aortic aneurysm of a patient. The inhibitor effectively eliminates the sex difference of the abdominal aortic aneurysm by specifically blocking the combination of CXCL14 and a receptor CXCR4 thereof, significantly reduces aortic macrophage infiltration and PDGFRA + macrophage accumulation in the progress process of the abdominal aortic aneurysm, and reduces the collagen degradation and elastic fiber destruction degrees, thereby inhibiting the pathological progress of the abdominal aortic aneurysm. The invention provides a new target spot and a medicine composition for precise treatment of male abdominal aortic aneurysm, and has a definite clinical application value.

The application belongs to the technical field of biological medicine, and specifically discloses a single-domain antibody against human MMP-1, a mesenchymal stem cell exosome targeted and modified by the single-domain antibody, and application of the single-domain antibody and the exosome in repairing skin photoaging. The surface of the exosome is covalently connected with the single-domain antibody that specifically targets human MMP-1 through click chemistry technology, the single-domain antibody has an amino acid sequence as shown in SEQ ID NO:1, and is obtained through camel phage display library screening and affinity maturation, and has a high affinity of a nanomolar level to MMP-1. The preparation method comprises screening and expression of the single-domain antibody, extraction and purification of the mesenchymal stem cell exosome, and directional coupling through DBCO-azide click chemistry. The modified exosome can specifically recognize and enrich in photoaged skin tissue, significantly inhibit collagen degradation, promote extracellular matrix reconstruction, relieve oxidative stress and inflammatory response, and exhibits excellent effects in repairing skin photoaging caused by ultraviolet rays, and is suitable for development of skin repair drugs and cosmetics.

The invention discloses application of roxburgh rose normal juice or probiotic fermentation products thereof in preparation of functional food or medicine for resisting light aging or regulating intestinal flora disorder, stable delivery of roxburgh rose active components in a dairy product multi-phase matrix is optimized, a delicious, accessible and standardized daily intervention carrier is constructed, and the roxburgh rose normal juice or the probiotic fermentation products thereof are applied to preparation of functional food or medicine for resisting light aging or regulating intestinal flora disorder. UVB / UVA / induced skin photoaging is intervened in multiple ways, collagen degradation is slowed down, pigment and inflammation phenotypes are improved, and the defects that existing prevention and treatment means are mainly used for external sunscreen and cosmeceuticals, and compliance and visible light protection are insufficient are overcome.

This invention provides a firming and anti-wrinkle serum and its preparation method, relating to the field of cosmetic technology. The serum comprises: a protein composition, superoxide dismutase (SOD), schistosome extract, budding stalk enzyme polysaccharide, and plant extracts; the protein composition consists of recombinant collagen and fibronectin. The protein composition and SOD form a closed loop of protection and repair, with the former replenishing collagen and filling wrinkles, and the latter scavenging free radicals and protecting collagen from degradation; schistosome extract moisturizes and locks in moisture, strengthening the skin barrier; budding stalk enzyme polysaccharide encapsulates active ingredients, promotes dermal penetration, and provides an immediate firming effect; plant extracts help inhibit collagen degradation and prevent dynamic wrinkles. This serum combines immediate firming and long-term anti-aging repair functions, and can be widely used in firming and anti-wrinkle cosmetics, providing an efficient solution for the field of cosmetic anti-aging.

The present application provides a kind of broad-spectrum UV protection and cosmetic light reverse light damage repair cosmetic composition and its application, belong to the cosmetic technical field.The cosmetic composition of the present application includes: photoactivated enzyme repair factor, ginger flower root extract and white water lily flower extract;The composition also includes QUERCARE light-resistant substance.The photoactivated enzyme repair factor, ginger flower root extract and white water lily flower extract of the present application are compounded, accelerate light damage recovery, improve sunscreen product performance, reduce adverse reactions after sun exposure, have the effect of soothing and whitening, light-resistant substance not only absorbs ultraviolet rays, and is converted into cosmetic light, from the source, the light that is harmful to skin is converted into the light that is beneficial to skin, has anti-inflammatory soothing effect, reduces collagen degradation, inhibits DNA damage and mutation, reduces cell nucleus senescence, inhibits oil secretion.The cosmetic composition of the present application is suitable for high sunscreen demand and anti-photoaging skin care products, has the function of immediate protection and long-term repair.