Alga-containing anti-aging repair composition and application thereof

Abstract

The invention provides an anti-aging repairing composition containing algae and application of the anti-aging repairing composition, the anti-aging repairing composition comprises blue-green algae, tripeptide and a bacillus fermentation product, and the mass ratio of the blue-green algae to the tripeptide to the bacillus fermentation product is (0.01-0.5): (0.1-1): (0.2-2). The composition obtained by compounding the blue-green algae, the tripeptide and the bacillus fermentation product has a synergistic effect in the aspect of resisting senility, especially in the aspect of promoting the secretion amount of collagen, and when the blue-green algae is replaced with other algae or the bacillus fermentation product is replaced with other fungus fermentation products, the effect of resisting senility is achieved. The effects of the composition disclosed by the invention cannot be achieved. Meanwhile, the composition provided by the invention also has multiple effects of repairing a barrier, resisting oxidation, resisting inflammation, promoting intercellular interaction, promoting collagen synthesis, inhibiting collagen degradation and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemicals, and particularly relates to an anti-aging and repair composition containing algae and its application. Background Art

[0002] Aging is divided into endogenous aging and exogenous aging, and photoaging is the most important exogenous aging. The prior art has disclosed a variety of anti-aging products, adding ingredients such as acetyl hexapeptide-8 with anti-wrinkle effects, peptides that can promote collagen synthesis, and hydroxyprolisilane that can promote the production of extracellular matrix to obtain anti-aging effects.

[0003] In addition to anti-aging, whether the product has functions such as repair is also a concern of current consumers. Summary of the Invention

[0004] In view of this, the technical problem to be solved by the present invention is to provide an anti-aging and repair composition containing algae and its application, and this composition has good anti-aging and repair effects.

[0005] The present application provides an anti-aging and repair composition containing algae, including cyanobacteria, tripeptide, and bacillus fermentation product, and the mass ratio of the cyanobacteria, tripeptide, and bacillus fermentation product is 0.01-0.5:0.1-1:0.2-2.

[0006] The present application uses cyanobacteria, tripeptide, and bacillus fermentation product in a compound manner, and the three produce a synergistic effect, having good anti-aging, repair, antioxidant, anti-inflammatory, promoting cell-cell interaction, and promoting collagen synthesis and inhibiting collagen degradation and other effects.

[0007] Cyanobacteria, with the English name CYANOBACTERIA, can mimic the action mechanism of endogenous growth factors, such as vascular endothelial growth factor VEGF, to promote epidermal repair and dermal microvascular repair. The present application has no special restrictions on the cyanobacteria, and cyanobacteria that can be purchased on the market and used as cosmetic raw materials can be used. In some specific implementation manners, the cyanobacteria can be the product obtained by pulverizing Phormidium uncinatum with the preservation number of CCAP1462 / 7.

[0008] In some specific implementation manners, the cyanobacteria can be prepared according to the following method:

[0009] (1) Cultivate the cyanobacteria under the condition of white light illumination;

[0010] (2) Pulverize the cultured cyanobacteria biomass and filter to obtain the supernatant;

[0011] (3) Mix the supernatant with an α-hydroxy acid salt and maltodextrin, and obtain blue algae powder after homogenization, filtration, and drying in sequence.

[0012] Specifically, in this application, blue algae in the exponential growth phase are first seeded and cultured at 25-26 °C under white light illumination, preferably for 10 to 20 days, to obtain blue algae biomass.

[0013] After the blue algae biomass is obtained by culturing, it is filtered, washed, and then crushed. The crushed blue algae biomass is filtered again to obtain the supernatant. Specifically, in this application, the blue algae biomass is first filtered through a sieve with a mesh size of 50 μm to 70 μm, preferably 60 μm, then washed in water, and then crushed under a pressure of 200 bar to 500 bar, preferably 300 bar to 400 bar, and then the crushed biomass is filtered through a membrane with a pore size of 0.1 μm to 0.5 μm, preferably 0.2 μm to 0.3 μm, to obtain the supernatant.

[0014] After the supernatant is obtained, it is mixed with an α-hydroxy acid salt and maltodextrin, homogenized, filtered again, and the supernatant is dried to obtain blue algae powder. Specifically, the α-hydroxy acid salt can be a lactate, such as calcium lactate or magnesium lactate, etc., preferably a composition of calcium lactate and magnesium lactate, and their mass contents in the blue algae powder are 0.5% to 1% and 1% to 1.5% respectively. The mass content of the maltodextrin in the blue algae powder is 5% to 7%. The supernatant is mixed with an α-hydroxy acid salt and maltodextrin, homogenized, and then filtered through a filter with a pore size of 0.1 μm to 0.5 μm, preferably 0.2 μm to 0.3 μm. After adjusting the pH value of the obtained supernatant to 6 to 7, preferably 6.5, the supernatant is dried to obtain blue algae powder.

[0015] Tripeptides, especially palmitoyl-modified tripeptides, mainly act on the dermis layer, promote the generation of extracellular matrix of fibroblasts, such as collagen, glycosaminoglycans, etc., and at the same time inhibit collagen degradation, which has a good effect on improving skin aging caused by photoaging, such as collagen loss and damage, etc. There is no special limitation on the tripeptides in this application, and palmitoyl-modified tripeptides are preferred, such as palmitoyl tripeptide-5. Palmitoyl tripeptide-5, with the English name Palmitoyl Tripeptide-5, also written as Pal-KTTKS, and the CAS number is 623172-55-4, promotes collagen generation by activating cell signaling pathways, significantly reduces wrinkles, improves skin firmness and luster, and shows potential in wound healing and tissue repair. There is no special limitation on the source of the palmitoyl tripeptide-5 in this application, and it can be purchased on the market.

[0016] Skin stem cells are the basis for maintaining skin function and repairing tissue damage. The endogenous stress factor cortisol can accelerate the damage of skin stem cells. The fermentation product of Bacillus can reduce the damage of stem cells caused by cortisol, thereby maintaining skin function and repairing tissue damage. There are no special restrictions on the fermentation product of Bacillus in this application, and it can be a cosmetic raw material purchased on the market. In some specific implementation manners, the fermentation product of Bacillus contains active ingredients such as γ-polyglutamic acid and / or metabolically small molecular polyamines, and the total content of the active ingredients is not less than 0.1%.

[0017] In some specific implementation manners, the mass ratio of the cyanobacteria, tripeptide and fermentation product of Bacillus is 0.01~0.25:0.1~0.6:0.2~1. In some specific implementation manners, the mass ratio of the cyanobacteria, tripeptide and fermentation product of Bacillus is 0.05~0.25:0.6:1. In some specific implementation manners, the mass ratio of the cyanobacteria, tripeptide and fermentation product of Bacillus is 0.25:0.6:1. In some specific implementation manners, the mass ratio of the cyanobacteria, tripeptide and fermentation product of Bacillus is 0.01:0.13:0.22. In some specific implementation manners, the mass ratio of the cyanobacteria, tripeptide and fermentation product of Bacillus is 0.05:0.6:1.

[0018] The composition obtained by compounding cyanobacteria, tripeptide and the fermentation product of Bacillus in this application has a synergistic effect in anti-aging, especially in promoting the secretion amount of collagen. When the cyanobacteria are replaced with other algae or the fermentation product of Bacillus is replaced with the fermentation product of other fungi, the efficacy of the composition disclosed in this application cannot be achieved. Specifically, the composition provided in this application can significantly increase the secretion amount of type I collagen. In addition, the composition obtained by compounding cyanobacteria, tripeptide and the fermentation product of Bacillus in this application can promote cell migration and has the efficacy of repairing the skin. The composition provided in this application can significantly up-regulate the gene expression related to keratinocyte differentiation and barrier, antioxidant / inhibition of inflammation, and cell interaction, and the up-regulation amplitude is greater than 150, with statistical differences, far higher than the control group. At the same time, the extracellular matrix metalloproteinase MMP3 is significantly reduced. The composition provided in this application can significantly up-regulate the gene expression of human primary fibroblast growth factor and cell interaction-related genes, and the up-regulation amplitude is greater than 150, with statistical differences, far higher than the control group. Therefore, the composition provided by the present invention has multiple effects such as barrier repair, antioxidant, anti-inflammatory, up-regulating cell interaction such as integrin and growth factor to promote healing (scratch experiment) and repair.

[0019] The present invention also provides a product, including: the anti-aging and repair composition described in the above technical solution.

[0020] In some embodiments of the present invention, the above products include, but are not limited to, personal care products, pharmaceuticals, etc. Those skilled in the art can understand that in addition to including the composition described in the above technical solution, other excipients are also included, such as pharmaceutical excipients or excipients for personal care products.

[0021] In some embodiments of the present invention, the personal care product includes: basic care products and / or makeup products.

[0022] In some embodiments of the present invention, the makeup products include, but are not limited to:

[0023] (1) Base makeup:

[0024] Liquid foundation / Cream foundation: Used to even out skin tone and cover blemishes;

[0025] BB cream / CC cream: Lightweight base makeup products with both skin care and modification functions;

[0026] Concealer / Corrector: Targeted at covering local areas such as pimples and dark circles;

[0027] Loose powder / Pressed powder: Sets makeup and reduces facial oiliness.

[0028] (2) Eye makeup:

[0029] Eyeshadow: Adds color and layering to the eyes;

[0030] Eyeliner pencil / Liquid eyeliner / Gel eyeliner: Outlines the eyeliner to make the eyes look more expressive;

[0031] Mascara: Lengthens and thickens the eyelashes and increases the depth of the eyes;

[0032] Eyebrow pencil / Eyebrow powder / Eyebrow gel: Fills in the gaps in the eyebrows and shapes the ideal eyebrow shape.

[0033] (3) Cheek makeup:

[0034] Blush: Adds natural color to the cheeks and improves complexion;

[0035] Contour powder / Contour stick: Uses shading techniques to make the facial contour more three-dimensional;

[0036] (4) Lip makeup:

[0037] Lipstick / Lip glaze / Lip gloss: Changes or emphasizes the color of the lips;

[0038] Lip liner: Draws a clear lip shape boundary to prevent lipstick from bleeding.

[0039] (5) Multifunctional makeup products:

[0040] Highlighter stick / Liquid highlighter / Powder highlighter: Highlights the high points of the face (such as the nose bridge and cheekbones) to create a shiny effect.

[0041] In addition, there are also products designed specifically for special occasions, such as waterproof and sweatproof eyeliner pencils, long-lasting lipsticks that do not fade, etc.

[0042] In some embodiments of the present invention, the basic skin care products include but are not limited to:

[0043] Facial cleanser / face wash: Gently removes facial dirt, oil and makeup residues;

[0044] Makeup remover oil / makeup remover water / makeup remover cream: Specifically used to thoroughly remove makeup, especially waterproof cosmetics;

[0045] Toner / lotion: Used after cleansing, it can further clean the residues on the skin surface, while replenishing moisture to the skin, restoring the skin's pH balance, and laying a good foundation for the absorption of subsequent skin care products;

[0046] Serum: Contains high-concentration active ingredients and provides deep nourishment and repair for specific skin problems (such as anti-aging, moisturizing, whitening, etc.);

[0047] Eye cream: Specifically designed for the sensitive area around the eyes, with the effects of reducing fine lines, dark circles and tightening the eye skin, and usually has a light and easy-to-absorb texture;

[0048] Day / night lotion or cream: Has functions such as protection, repair, nourishment, and promotion of cell regeneration, can provide necessary moisture to the skin and lock in the replenished moisture;

[0049] Sunscreen: Used for ultraviolet protection to prevent photoaging, etc.;

[0050] Facial mask: Brings extra nourishment and care to the skin, such as moisturizing, cleaning pores or brightening the skin tone, etc.

[0051] In this application, cyanobacteria, tripeptide and Bacillus fermentation products are used in combination, and the three produce a synergistic effect, having good anti-aging, repair, antioxidant, anti-inflammatory and other effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 It is a statistical chart of the test results of type I collagen in vitro;

[0053] Figure 2 It is the cell scratch test result provided by Test Example 2. DETAILED DESCRIPTION OF THE INVENTION

[0054] The present invention provides an anti-aging and repair composition containing algae and its application. Those skilled in the art can draw on the content of this article and appropriately improve the process parameters to achieve it. The methods and applications of the present invention have been described through preferred embodiments. Relevant personnel can obviously make changes or appropriate modifications and combinations to the methods and applications in this article without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.

[0055] The present application provides an anti-aging and repair composition containing algae, including cyanobacteria, tripeptide and bacillus fermentation product. The mass ratio of the cyanobacteria, tripeptide and bacillus fermentation product is 0.01-0.5:0.1-1:0.2-2.

[0056] The present application uses cyanobacteria, tripeptide and bacillus fermentation product in combination, and the three produce a synergistic effect, having good anti-aging, repair, antioxidant, anti-inflammatory, promoting cell-cell interaction, and promoting collagen synthesis and inhibiting collagen degradation and other effects.

[0057] The composition obtained by combining cyanobacteria, tripeptide and bacillus fermentation product in the present application has a synergistic effect in anti-aging, especially in promoting the secretion amount of collagen. When cyanobacteria are replaced with other algae or the bacillus fermentation product is replaced with other fungal fermentation products, the effects of the composition disclosed in the present application cannot be achieved. Specifically, the composition provided by the present application can significantly increase the secretion amount of type I collagen. In addition, the composition obtained by combining cyanobacteria, tripeptide and bacillus fermentation product in the present application can promote cell migration and has the effect of repairing the skin. The composition provided by the present application can significantly up-regulate the gene expression related to keratinocyte differentiation and barrier, antioxidant / inhibiting inflammation, and cell-cell interaction, and the up-regulation amplitude is greater than 150, with statistical significance and much higher than the control group. At the same time, the extracellular matrix metalloproteinase MMP3 is significantly reduced. The composition provided by the present application can significantly up-regulate the gene expression of human primary fibroblast growth factor and cell-cell related interaction, and the up-regulation amplitude is greater than 150, with statistical significance and much higher than the comparative example group. Therefore, the composition provided by the present invention has multiple effects such as barrier repair, antioxidant, anti-inflammatory, up-regulating cell-cell interaction such as integrin and growth factor to promote healing (scratch test) and repair.

[0058] The following further illustrates the present application in conjunction with examples.

[0059] In the following examples, the cyanobacteria are prepared according to the following steps:

[0060] (1)Using Phormidium uncinatum with the preservation number CCAP1462 / 7 as the raw material, during the exponential growth stage of cyanobacteria culture, pre-culture of 250 - 20,000 ml was carried out by continuous seeding. The culture temperature was 25 - 26 °C, with white light illumination, and a 130 L transparent plastic tube was used as the culture dish. After 10 - 20 days of culture, cyanobacteria biomass was obtained;

[0061] (2)After the cyanobacteria biomass was filtered through a 60 μm sieve, it was washed and continuously crushed several times under a pressure of 400 bar. The ground cyanobacteria biomass was filtered through a 0.2 μm membrane to obtain the supernatant;

[0062] (3)The supernatant was mixed with 5% - 7% of maltodextrin based on the total weight of the supernatant, 1% - 1.5% of magnesium lactate based on the total weight of the supernatant, and 0.5% - 1% of calcium lactate based on the total weight of the supernatant, and homogenized to obtain a uniform solution. After filtering through a 0.2 - micron sterile filter, the pH was adjusted to 6.5, and cyanobacteria powder was obtained after drying.

[0063] The tripeptide is palmitoyl tripeptide - 5, a commercially available product provided by READLINE Biotechnology Co., Ltd.;

[0064] The Bacillus fermentation product is a commercially available product provided by Guangzhou Roundex Chemical Co., Ltd.;

[0065] The extract of Oscillatoria iranica is a commercially available product provided by Lica Biotechnology Group;

[0066] The lysate of Saccharomyces cerevisiae ferment is a commercially available product provided by Guangzhou Yanfu Biotechnology Co., Ltd.

[0067] Examples 1 - 2, Comparative Examples 1 - 5

[0068] The raw materials were mixed according to the formula shown in Table 1 to obtain the composition.

[0069] Table 1 Formulas of the compositions provided by examples and comparative examples

[0070]

[0071] Test Example 1

[0072] 1. Cell seeding

[0073] Fibroblasts were selected. After cell resuscitation, the cells were seeded into a 6 - well plate and incubated overnight in a CO2 incubator (37 °C, 5% CO2).

[0074] 2. Test grouping

[0075] The experiment set up a blank control group BC, a negative control group NC, a positive control group PC and a sample group, with 3 replicate wells in each group. The grouping is shown in Table 2. In Table 2, the test samples for each group were the corresponding compositions diluted with DMEM culture medium (Gibco) to make the final concentration meet the dosing doses required in Table 2.

[0076] Table 2 Grouping of Test in Experiment 1

[0077]

[0078] 3. H2O2 Stimulation

[0079] According to the test grouping, when the cell seeding rate in the 6-well plate reached 40% - 50%, except for the blank control group, the other groups were all stimulated with H2O2 for 2 hours of induction.

[0080] 4. Administration of Drugs

[0081] After the induction time ended, grouping administration was carried out according to the test grouping, with 3 replicate wells in each group. 2 mL of culture medium was added to each well in the blank control group and the negative control group, 2 mL of culture medium containing quercetin was added to each well in the positive control group, and 2 mL of culture medium containing the corresponding concentration of the sample to be tested was added to each well in the sample group. After the administration was completed, the 6-well plate was placed in a CO2 incubator (37°C, 5% CO2) for 24 hours of culture. The above-mentioned stimulation and drug administration steps were repeated 3 times.

[0082] 5. Immunofluorescence Detection of Type I Collagen (COLⅠ)

[0083] After the incubation ended, it was washed. After the washing ended, it was fixed with 4% paraformaldehyde for 30 minutes, and then immunofluorescence detection was carried out. Photos were taken and observed under a microscope, and pictures were collected and analyzed.

[0084] 6. Calculation of Promotion Rate

[0085] The promotion rate of type I collagen was calculated according to the following formula:

[0086] Promotion rate (%) = 100% × (sample group - negative control group) / negative control group;

[0087] 7. Statistical Analysis of Results

[0088] The t-test statistical analysis was used for comparison between groups, and all statistical analyses were two-tailed. p < 0.05 was considered to have a significant difference.

[0089] The results are shown in Table 3 and Figure 1 , Table 3 is the statistical table of the test results of type I collagen in vitro, Figure 1 and is the statistical chart of the test results of type I collagen in vitro.

[0090] Table 3 Statistical Table of in vitro Type I Collagen Test Results

[0091]

[0092] Note: The relative integrated optical density (IOD) value reflects the content of Collagen Ⅰ. When performing statistical analysis using the t-test method, compared with the BC group, significance is indicated by #, p < 0.05 is indicated by #, and p < 0.01 is indicated by ##. Compared with the NC group, significance is indicated by *, p < 0.05 is indicated by *, and p < 0.01 is indicated by **.

[0093] From Table 3 and Figure 1 it can be seen that:

[0094] Compared with the BC group, the secretion of type I collagen in the NC group decreased significantly, indicating that the stimulation conditions of this test are effective. Compared with the NC group, the secretion of type I collagen in the PC group increased significantly, indicating that the positive control of this test is effective.

[0095] According to the synergy calculation formula q = E A+B / (E A +E B -E A ×E B ), when Example 1 is compared with Comparative Example 1 and Comparative Example 2, q is 1.25. When Example 2 is compared with Comparative Example 5 and Comparative Example 2, q is 1.14. That is, at the test concentration, the combination of tripeptide, Bacillus fermentation product and cyanobacteria produced a synergistic effect.

[0096] In addition, in Comparative Example 3, the extract of Phormidium iranicum was used to replace cyanobacteria, and in Comparative Example 4, the lysate of Saccharomyces boulardii fermentation product was used to replace the Bacillus fermentation product. After t-test detection (statistical analysis is two-tailed), compared with the promotion rate of Example 1, in Comparative Example 3, p = 0.015 (p < 0.05, there is a significant difference), and compared with Example 1, in Comparative Example 4, p = 0.000 (p < 0.01, there is a significant difference).

[0097] The above results mean that cyanobacteria, tripeptide and Bacillus fermentation product produced a synergistic effect at the test concentration, and replacing cyanobacteria or fermentation product in the above combination with the extract of Phormidium iranicum or the lysate of Saccharomyces boulardii fermentation product respectively could not achieve the above effect.

[0098] Test Example 2

[0099] Cell scratch test is widely used to evaluate the repair and cell migration promotion effects of raw materials in vitro.

[0100] 1. Cell culture: Immortalized keratinocytes HaCaT were used. The cells were resuscitated and incubated in a CO2 incubator (37°C, 5% CO2) for 24 hours after cell seeding.

[0101] 2. Experimental grouping: The blank control group NT was not treated. For the other groups, as shown in Table 4, in Table 4, the test samples for each group were the corresponding compositions diluted with DMEM medium (Gibco) to make the final concentration meet the dosing concentration requirements in Table 4.

[0102] Table 4 Test grouping for Experiment 2

[0103]

[0104] 3. Scratch treatment: Scratches were made on the cells, and the test samples were added. Photos were taken and recorded at 0, 6, and 24 hours after sample addition.

[0105] 4. The results are shown in Figure 2 , Figure 2 which are the cell scratch test results provided for Test Example 2. As can be seen from Figure 2 , at 24 hours, Example 3 could promote cell migration, which was better than Comparative Example 6, indicating that cyanobacteria, tripeptide, and Bacillus fermentation products had the effects of promoting cell migration and repairing the skin under the test conditions.

[0106] Test Example 3

[0107] 1. Cell culture: Human keratinocytes NHEK were seeded in 24-well plates and cultured in the medium for 24 hours. The culture conditions were: 37°C × 5% CO2.

[0108] 2. Test grouping: The blank control group NT was not treated. The others were carried out according to Table 5. In Table 5, the test samples for each group were the corresponding compositions diluted with DMEM medium (Gibco) to make the final concentration meet the dosing concentration requirements in Table 5.

[0109] Table 5 Test grouping for Experiment 3

[0110]

[0111] 3. Drug administration: As shown in Table 5, the medium was replaced with the culture solution containing the test sample and incubated for 24 hours. All experimental conditions were carried out with n = 3. At the end of incubation, the cells were collected and immediately dry-frozen at 80°C.

[0112] 4. Differential expression analysis: Total RNA was extracted from the cells collected under each experimental condition using TriPure Isolation Reagent, reverse-transcribed into cDNA, and the expression of the target gene was detected by RT-qPCR method.

[0113] The PCR (Polymerase Chain Reaction) reaction mixture (final volume 10 μL) was prepared as follows:

[0114] 2.5 μL of cDNA template;

[0115] 2 μL of primers (forward and reverse), as shown in Table 6;

[0116] 5.5 μL of reaction buffer;

[0117] Each experimental group and the untreated group had 3 replicate samples respectively.

[0118] Table 6 Primers for target genes

[0119]

[0120] 5. Data processing: The raw data was analyzed using Microsoft Excel software. The PCR cycle value of the target gene of each sample was subtracted by the GAPDH cycle value of its respective sample, and the average value of the 3 samples in each group was taken. Then, the average cycle value of the experimental group was subtracted by that of the untreated group to obtain the relative gene expression level. A relative expression level higher than 150% indicated a statistically significant difference.

[0121] The results are shown in Table 7 and Table 8. Table 7 shows the results of the effects of the test samples on the gene expression of human primary keratinocytes, and Table 8 shows the results of the effects of the test samples on the gene expression of human primary fibroblasts.

[0122] Table 7 Results of the effects of the test samples on the gene expression of human primary keratinocytes

[0123]

[0124] Compared with Comparative Examples 7 and 8, Example 4 could significantly up-regulate the gene expression related to the differentiation and barrier-related pathways, antioxidant / inhibition of inflammation, and cell interaction of human primary keratinocytes, and the up-regulation amplitude was greater than 150, showing a statistically significant difference and being much higher than the comparative example group. At the same time, the extracellular matrix metalloproteinase MMP3 was significantly reduced. The research results suggest that the composition provided by the present invention has the effects of barrier repair, antioxidant, promoting cell interaction, and inhibiting cell matrix degradation, etc.

[0125] Table 8 Results of the effects of the test samples on the gene expression of human primary fibroblasts

[0126]

[0127] Compared with Comparative Examples 7 and 8, Example 4 can significantly up-regulate the expression of human primary fibroblast growth factor and genes related to cell-cell interactions, and the up-regulation amplitude is greater than 150, showing a statistical difference and being much higher than the comparative example group. The research results suggest that the composition provided by the present invention can promote healing (scratch test) and repair and other effects by up-regulating cell-cell interactions such as integrin and growth factor.

[0128] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. An anti-aging and repair composition containing algae, comprising cyanobacteria, tripeptide and bacillus fermentation product, wherein the mass ratio of the cyanobacteria, tripeptide and bacillus fermentation product is 0.01-0.5:0.1-1:0.2-2.

2. The anti-aging repair composition according to claim 1, wherein The mass ratio of the cyanobacteria, tripeptide and bacillus fermentation product is 0.01-0.25:0.1-0.6:0.2-1.

3. The anti-aging repair composition according to claim 2, characterized in that, The mass ratio of the cyanobacteria, tripeptide and bacillus fermentation product is 0.05-0.25:0.6:1 or 0.01:0.13:0.

22.

4. The anti-aging and repair composition according to claim 3, wherein The mass ratio of the cyanobacteria, tripeptide and bacillus fermentation product is 0.25:0.6:1 or 0.05:0.6:

1.

5. The anti-aging repair composition according to any one of claims 1 to 4, characterized in that, The cyanobacteria is the cyanobacteria with the preservation number of CCAP 1462 / 7; The tripeptide is palmitoyl tripeptide-5.

6. Use of the anti-aging and repair composition according to any one of claims 1-5 in the preparation of an anti-aging product or a repair product.

7. Use of the anti-aging and repair composition according to any one of claims 1-5 in the preparation of a product for promoting collagen synthesis or a product for promoting cell migration.

8. Use of the anti-aging and repair composition according to any one of claims 1-5 in the preparation of an antioxidant and / or anti-inflammatory product.

9. Product, characterized in that, Comprising: The anti-aging and repair composition according to any one of claims 1-5.

10. The product according to claim 9, characterized in that, Including personal care products.

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