104 results about "Bacillus sp. ET" patented technology

The invention discloses a novel compound microorganism living bacteria agent. The agent comprises probiotics such as pediococcus, saccharomyces, bacillus and the like, wherein the total bacterial quantity is above 107 colony-forming units (cfu)/gram. The invention further discloses a method for preparing the novel compound microorganism living bacteria agent and applications of the novel compound microorganism living bacteria agent in preparation of organic sewage purification additives, deodorization fly dispelling agents, degreasing agents, pipeline blockage removing agents, feed additives and microorganism disease prevention agents. The novel compound microorganism living bacteria agent has the comprehensive effects of high removal rates to ammonia nitrogen, total nitrogen and total phosphorus, small sludge residues, energy saving, environmental friendliness and the like and the characteristics of convenience in use, safety and quickness in function, no secondary pollution, benefits to ecological protection, low treatment cost, good comprehensive profit and the like.

The invention relates to a method for preparation of immobilized complexion bacterial agent used as controlling the secondary pollution to river bottom mud, which is characterized in that individually prepare photosynthetic bacterium fermenting culture liquid and bacillus fermenting culture liquid, and prepare immobilized complexion bacterial agent in solid fermenting culture medium. The immobilized complexion bacterial agent is not easy to leak after putting into the polluted river, not only exerting the degradation of microbial bacterial agent to contaminant, and also exerting the adsorption and immobilization of zeolitic powder to the contaminant given off by bottom mud, and thus gaining the ends of decreasing the bottom mud pollution to superjacent water. The tests indicate that the pollution deactivation can decreases dramatically with the immobilized complexion bacterial agent developed in the invention.

The invention relates to a mixed bacterial agent for reducing viscosity of thick oil, as well as a preparation method and a use thereof, belonging to the technical field of crude oil recovery. The mixed bacterial agent for reducing the viscosity of the thick oil comprises xanthomonas, pseudomonas, microbacterium and bacillus, and the volume ratio of fermentation solutions of all bacterial genera is (20-100):(40-180):(30-100):(10-80). The preparation method comprises the following steps: respectively fermenting and mixing first-stage seed solutions of all bacteria: (1) getting strains from sources: extracting the strains out of water of Bohai sea gulf, and taking thick crude oil as a carbon source for acclimation and screening; (2) preparing the first-stage seed solutions: respectively activating on a peptone-yeast slant culture medium and transferring into a liquid peptone-yeast culture medium for shaking and activation; and (3) preparing a fermentation solution of mixed bacteria: respectively transferring the first-stage seed solutions of the strains into a fermentation culture medium for culture, wherein the living bacteria count in the mixed bacteria is 10<8>-10<10> bacteria/ml. The mixed bacterial agent for reducing the viscosity of the thick oil is used for reducing the viscosity of the crude oil. The mixed bacterial agent for reducing the viscosity of the thick oil, disclosed by the invention, has the advantages of high activity, rapid growth, no secondary pollution, good viscosity-reducing effect and the like.

The invention provides a holothuria scabra artificial seeding breeding method. The method comprises the steps such as parent holothurian cultivating, induced spawning, spawn washing and hatching, larva cultivating, seedling collecting and childish holothurian cultivating, wherein spirulina powder, marine yeast, aquatic yeast and bacillus serve as bait, not only are bait sources convenient, but also the investment of single cell culture facilities is saved, meanwhile, manpower needed by single cell algae bait culture is saved, the holothurian seedling cost can be greatly saved, and the popularization of the holothuria scabra seedling breeding technology is facilitated. The substitutive bait is adopted to replace living single cell algae, invested funds of seeding facilities can be saved by about 25 percents, the human input of seeding can be saved by about 30 percents, and the ratio of the seeding input to the seeding output 1:3. Induced spawning is performed on the two days before or after the first day and the fifteenth day of a lunar month, the good effect of two times of spawn sampling in one month is achieved, the induced spawning success rate is 83.3 percents, and the technical problem that the induced spawning difficulty of holothuria scabra parent holothurian is high is effectively solved.

The invention relates to a special biological disease-resistant vermin-proof culture medium for flowers and a preparation method thereof. The special biological disease-resistant vermin-proof culture medium for flowers is prepared from smoke dross, furfural residue, microbial fermentation inoculum, superphosphate, trichoderma microbial inoculum, bacillus microbial inoculum and expanded perlite. The preparation method comprises the following steps: compounding smoke dross, furfural residue, microbial fermentation inoculum and superphosphate; after the mixture is decomposed, sterilizing, adding trichoderma microbial inoculum and bacillus microbial inoculum with disease-resistant function; and after composting for some time, adding the expanded perlite. Abundant active trichoderma and bacillus and metabolites thereof contained in the culture medium provided by the invention have obvious inhibiting action on flower diseases; and the smoke residue and resolvents thereof have the action of expelling and killing vermins which are easily generated in the medium. When being used for planting flowers, the special culture medium for flowers can protect the environment, and is beneficial to human health.

A nutritive bio-additive for feed of livestock and fowls contains fermented product of lysine, bacillus and photosynthetic bacteria, bean cake, plant starch and trace elements. Its advantages are high biological effect-cost ratio, high effect on decomposing harmful substances in animal body, and rich methionine and somatic protein for high immunity.

The invention relates to a high-efficiency extraction method of cannabidiol, and belongs to the technical field of extraction methods of plant effective components. In order to solve the problems of low extraction efficiency, low product purity and low yield of an existing cannabidiol extraction method, the invention provides the high-efficiency extraction method of cannabidiol. The method comprises the following steps: fermentating a hemp raw material by trichoderma harzianum, carrying out ultrahigh-pressure treatment, carrying out fermentation by bacillus polymyxa, carrying out washing and centrifugation, removing impurities by macroporous adsorption resin, and carrying out purification by a silica gel chromatography column, so that a cannabidiol solid with purity of 98% is finally obtained, and the tetrahydrocannabinol impurity is not detected. When cannabidiol is extracted from hemp by using the method, a pretreatment method combining fungus solid fermentation, ultrahigh pressure treatment and bacillus solid fermentation is adopted, an enzyme system generated by microbial fermentation metabolism can destroy the dense physical and chemical structure in hemp cells, and the hemp cells are thoroughly crushed by ultrahigh-pressure treatment, so that cannabidiol is fully released, and the extraction rate of cannabidiol and the utilization rate of hemp can be remarkably improved.

The invention discloses a protein feed and a preparation method of the protein feed. The preparation method comprises the following steps of: mixing bean pulp, water, rice bran and bran according to a weight proportion of 5-7:5-7:1:1, and obtaining a base material; mixing lactobacillus plantarum, saccharomyces cerevisiae and bacillus according to a weight proportion of 0.9-1.1:0.9-1.1:1, and obtaining a bacterial solution with the total bacterium number more than 109 CFU/ml; and preparing a fermentation substance by 100 parts of base material, 10-20 parts of white popinac leaf, 7.5-15 parts of bacterial solution and 5-6 parts of brown sugar or white sugar, or 100 parts of base material and 20-50 parts of white popinac leaf, wherein when more than 20 parts of white popinac leaf is added, 0.05-0.08 part of 0.4 wt% ferric sulfate solution, 7.5-15 parts of bacterial solution and 5-6 parts of brown sugar or white sugar are added at every five parts of white popinac leaf. The protein feed is high in protein content, low in mimosine content, simple in technology, low in cost and pollution-free.

The invention provides a composite microbial disease/insect-resistant agent for green vegetables and a manufacturing method thereof. The composite microbial disease/insect-resistant agent for green vegetables comprises a composite microbial inoculant and a fertilizer, wherein the composite microbial inoculant is a mixed microbial inoculant formed by fermenting Bacillus and Rhodospirillum in a culture medium; the fertilizer comprises a potassium fertilizer and a pH regulator; the mass ratio of the composite microbial inoculant to the fertilizer is (30-50):(50-70); and the mass ratio of the Rhodospirillum microbial inoculant to the Bacillus microbial inoculant is (1-3):1. By adopting the composite microbial inoculant, the agent has the advantages of sufficient nutrients and reasonable supply, can enhance the nutrient absorption capacity of the plant root, and effectively inhibits harmful bacteria from growth and reproduction. When being used for a long time, the agent can improve the soil and avoid multiple diseases and insects due to deficiency of nutrient elements.

The invention relates to a main material for constructing a tillage layer of newly rectified cultivated land, a construction method of the tillage layer and application. The main material for constructing the tillage layer of the newly rectified cultivated land comprises peat materials, active organic materials and biological activators; the peat materials include herbal peat, woody peat and/or humus soil; the active organic materials are natural plant organic materials rich in polysaccharide/organic acid/amino sugar; the biological activators comprise amino acid bacteria and bacillus which promote degradation of all the active organic materials to form soil organic matter, facilitate formation of a good agglomeration structure of the soil and activate rapid circulation of the system. Thetillage layer is rapidly constructed by engineering, a traditional mode is subverted, a lengthy cultivation and ripening process formed by the high-quality tillage layer can be simplified into one-time engineering operation, geotechnical cultivation of the newly rectified cultivated land is improved in a short time, and crop yields and benefits are continuously ensured.

A strain of Lactobacillus salivarius isolated from resected and washed human gastrointestinal tract inhibits a broad range of Gram positive and Gram negative microorganisms and secretes a product having antimicrobial activity into a cell-free supernatant. The activity is produced only by growing cells and is destroyed by proteinase K and pronase E, the inhibitory properties of the strain and its secretory products being maintained in the presence of physiological concentrations of human bile and human gastric juice. The strain exhibits a broad-spectrum of activity against bacteria including Listeria, Staphylococcus, including methocillin resistant St. aureus (MRSA), and Bacillus, but does not inhibit many closely related lactobacilli. An antimicrobial agent is obtained from the strain which has bacteriocin-like properties.

The invention provides a method for labeling and culturing a penaeus japonicus family in the same pond of a higher-place pond and relates to the cultivation of young shrimps. Sand is paved in the higher-place pond and the higher-place pond is disinfected; a pipeline device for inflating and oxygenating is mounted, and seawater is filtered by the paved sand; the whole pond is disinfected, and a nutrition preparation and a bacillus viable bacterium preparation are sprayed; a fixed suspension net box is produced; the young shrimps are selected and transported; after the young shrimps are put into the pond, the water level is raised; the bacillus viable bacterium preparation and an organic fertilizer are sprayed, and povidone iodine is sprayed to disinfect a water body; after the young shrimps are put into the pond, fairy shrimp larvae which are manually incubated are fed in the morning and at night of each day within first 10 days; during a cultivation post-period, a young shrimp particle feed added with vitamins, amino acids and a lactobacillus viable bacterium preparation is fed; during the cultivation post-period, a water sample is taken every 5 days to observe the quantity of vibrio; when a lot of the vibrio is bred, a disinfectant is used for disinfecting the higher-place pond and antibiotics are added into a bait; when the young shrimps grow to be about 4cm, a fluorescent mark is injected to carry out family marking, and all the marked families are put into the same higher-place pond to be poly-cultured, and then the same pond property testing experiment is carried out.

This invention describes the discovery and synthesis of N-thiolated 2-oxazolidinones as a new class of anti bacterial agents. These compounds can be synthesized from 2-oxazolidinones by Ndeprotection and N-sulfenylation. These new substances were found to exhibit potent anti-bacterial activity, including bacteriostatic properties against Staphylococcus spp., including methicillin resistant Staphylcoccus aureus (MRSA), and Bacillus spp., including Bacillus anthracis.

The invention relates to a feedstuff additive prepared from bacillus antibacterial peptides and bacillus probiotics and a using method thereof in feedstuff, and belongs to the field of feedstuff additives. The compound feedstuff additive is prepared from bacillus antibacterial peptides and bacillus probiotics, wherein the content of bacillus antibacterial peptides in per gram of compound feedstuff additive is 2-20 mg, and the content of bacillus probiotics in per gram of compound feedstuff additive is 1.0-5.0*109 cfu. The feedstuff additive can be used as an antibiotic substituted product, antibiotic residues are removed, the quality of meat, eggs and milk is improved, and the feedstuff additive can be used for preventing and treating kinds of animal diarrhea, improving production performance of animals, immunity and disease resistance and increasing the feedstuff conversion rate. The additive is a green feedstuff additive and is beneficial to improving the quality of livestock and poultry products and health of humans.

The invention discloses a strawberry growing technology. Through years of growing experience, the proportion of nitrogen to phosphorus to potassium in each growing process is matched reasonably; during fertilization, a thin-fertilizer frequent-fertilization method is adopted, so that each nutrient element can be supplemented in time in each growing process of strawberries, and the grown strawberries are bright in color and luster, uniform in shape and fine in mouthfeel; specific to the problem of year-by-year production reduction troubling farmer households, a rare earth micro fertilizer is applied additionally at a fruiting stop period, and inorganic phosphorus potassium in soil is decomposed through potassium bacteria and bacillus to increase the permeability of soil, so that the strawberry yield of the next year will not be lowered.

The invention relates to a novel biological floc. The biological floc includes microalgae aggregates and commensalism heterotrophic bacteria, and the commensalism heterotrophic bacteria are Pesudomonas sp. and Bacillus sp. The biological floc significantly improves the water quality of the nursing pond of the litopenaeus vannamei, improves the survival rate, growth index and immunity of the litopenaeus vannamei, reduces the application amount of compound feed and external carbon sources, and reduces the aeration intensity and quantity of exchanged water.