High-efficiency extraction method of cannabidiol

A technology of cannabidiol and an extraction method, which is applied in the field of high-efficiency extraction of cannabidiol, can solve the problems of low yield, low product purity, and low extraction efficiency of cannabidiol, and achieves high purity, improved extraction rate, and reduced pressure. Effect

Active Publication Date: 2019-10-29
李卫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the existing problems of low extraction efficiency, low product purity and ...

Method used

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  • High-efficiency extraction method of cannabidiol

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Embodiment 1

[0031] A high-efficiency extraction method of cannabidiol, comprising the steps of:

[0032] Step 1, dry hemp leaves and hemp flowers and crush them to 40 mesh to obtain hemp powder; prepare a Trichoderma harzianum liquid fermentation medium, and add the Hemp Hemp powder, sterilized in a sterilizing pot at 120°C for 20 minutes, cooled for later use;

[0033] Step 2, after Trichoderma harzianum is activated, insert the liquid seed culture medium, carry out seed cultivation at a certain temperature to obtain the Trichoderma harzianum seed liquid; insert the Trichoderma harzianum seed liquid into the cooled Trichoderma harzianum liquid obtained in step 1 with a certain inoculum amount Fermentation medium, after mixing, carry out solid culture under certain temperature and humidity conditions to obtain Trichoderma harzianum fermentation culture; place the obtained Trichoderma harzianum fermentation culture in a sterilizing pot at 120°C for 40 minutes, cool, fully dry and pulverize...

Embodiment 2

[0041] The difference between this embodiment and Embodiment 1 is that step 1 in this embodiment is as follows:

[0042] Step 1. Dry the hemp leaves and hemp flowers at 80°C for 10-12 hours, and then crush them to 40 mesh to obtain hemp powder; prepare the Trichoderma harzianum liquid fermentation medium, and add the hemp powder with a material-to-liquid ratio of 1g: 1mL In the Trichoderma harzianum liquid fermentation medium, sterilize in a sterilizing pot at 120°C for 20 minutes, and cool down for use.

[0043] The formula of Trichoderma harzianum liquid fermentation medium in this embodiment is: potato 200g / L, sucrose 10g / L, glucose 10g / L, pH6.5.

Embodiment 3

[0045] The difference between this embodiment and embodiment 2 is that step 2 in this embodiment is as follows:

[0046] The slant culture after the activation of Trichoderma harzianum was inserted into the liquid seed medium, and cultivated at 25°C for 48h to obtain a Trichoderma harzianum seed liquid with a mycelium concentration of 200-250g / L; 10% to 15% of the volume of the Trichoderma harzianum liquid fermentation medium, the Trichoderma harzianum liquid fermentation medium obtained in step 1 into the cooled Trichoderma harzianum liquid fermentation medium, mixed at 28 ° C, and the relative humidity is 92% After culturing for 5 days, the obtained Trichoderma harzianum fermentation culture was sterilized in a sterilizing pot at 120° C. for 40 minutes, cooled, fully dried and crushed to 40 mesh for use.

[0047] Trichoderma harzianum liquid seed medium formula is in the present embodiment: potato 200g / L, sucrose 10g / L, glucose 10g / L, peptone 1g / L, anhydrous sodium acetate 2...

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Abstract

The invention relates to a high-efficiency extraction method of cannabidiol, and belongs to the technical field of extraction methods of plant effective components. In order to solve the problems of low extraction efficiency, low product purity and low yield of an existing cannabidiol extraction method, the invention provides the high-efficiency extraction method of cannabidiol. The method comprises the following steps: fermentating a hemp raw material by trichoderma harzianum, carrying out ultrahigh-pressure treatment, carrying out fermentation by bacillus polymyxa, carrying out washing and centrifugation, removing impurities by macroporous adsorption resin, and carrying out purification by a silica gel chromatography column, so that a cannabidiol solid with purity of 98% is finally obtained, and the tetrahydrocannabinol impurity is not detected. When cannabidiol is extracted from hemp by using the method, a pretreatment method combining fungus solid fermentation, ultrahigh pressure treatment and bacillus solid fermentation is adopted, an enzyme system generated by microbial fermentation metabolism can destroy the dense physical and chemical structure in hemp cells, and the hemp cells are thoroughly crushed by ultrahigh-pressure treatment, so that cannabidiol is fully released, and the extraction rate of cannabidiol and the utilization rate of hemp can be remarkably improved.

Description

technical field [0001] The invention belongs to the technical field of plant active ingredient extraction methods, in particular to a high-efficiency extraction method of cannabidiol. Background technique [0002] Marijuana has become one of the original drugs known to the public because it contains a hallucinogenic and addictive secondary metabolite-tetrahydrocannabinol (THC). In order to facilitate supervision and rational use, hemp varieties with a THC content of less than 0.3% in hemp are internationally defined as industrial hemp with no drug utilization value, also known as hemp. [0003] Cannabidiol (CBD), a non-addictive component in hemp, has the functions of protecting nerves, improving memory, anti-inflammation, sterilization, analgesia, anti-anxiety, anti-psychotic, and anti-oxidation, and has high research value. The traditional extraction method of cannabidiol is solvent extraction, because the cannabinoid components in the hemp plant are complex and have simi...

Claims

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Application Information

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IPC IPC(8): C07C37/68C07C39/23
CPCC07C37/004C07C2601/16C07C39/23
Inventor 孟庆刚李卫赵立涛孙宇峰付荣太杜继伟孟鑫岳琦吕明明李宝林
Owner 李卫
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