7059 results about "Chromatography column" patented technology

Rapid characterization and screening of polymer samples to determine average molecular weight, molecular weight distribution and other properties is disclosed. Rapid flow characterization systems and methods, including liquid chromatography and flow-injection analysis systems and methods are preferably employed. High throughput, automated sampling systems and methods, high-temperature characterization systems and methods, and rapid, indirect calibration compositions and methods are also disclosed. In preferred high-temperature embodiments, the polymer sample is maintained at a temperature of not less than about 75° C. during sample preparation, loading into a liquid chromatography or flow-injection analysis system, injection into a mobile phase of a liquid chromatography or flow-injection analysis system, and / or elution from chromatographic column. The described methods, systems, and device have primary applications in combinatorial polymer research and in industrial process control.

The present inventors devised methods for efficiently purifying bispecific antibodies using a chromatography column based on the difference in isoelectric points between the H chains of two types of antibodies, wherein the difference is introduced by modifying the amino acids present on the surface of the antibody variable regions of two types of antibodies that constitute a bispecific antibody. Furthermore, the inventors devised methods for efficiently purifying bispecific antibodies using a chromatography column by linking respective antigen binding sites (heavy chain variable regions) to the antibody constant regions having different isoelectric points, and then coexpressing these antibodies.

The present invention is directed to a microfluidic chromatography apparatus comprising a microfabricated fluid delivery system and a chromatography column which is in fluid communication with the fluid delivery system, and a method for producing and using the same. Preferably, the chromatography column comprises an OTLC, PCLC, or combinations thereof.

The present invention relates to a method of separating antibodies from other compound(s) in a liquid sample, wherein a mobile phase comprising said sample is contacted with a multi-modal separation matrix to adsorb undesired compounds while the antibodies remain free in the liquid, wherein the multi-modal separation matrix comprises first groups, which are capable of interacting with negatively charged sites of the target compounds, and second groups, which are capable of at least one interaction other than charge-charge interaction with said target compounds. The invention also relates to a chromatography column packed with the above-described multi-modal separation matrix and a filter having such multi-modal groups adsorbed to its surface.

An auto-injection system provides high throughput screening of fluidic samples. A sample injection valve has a first position which applies a reduced pressure to a sample sipper tube for aspirating a fluidic sample into the sample sipper tube, and a second position which delivers the fluidic sample to a sample supply loop. A column control valve has a first position which delivers the fluidic sample from the sample supply loop to a sample chromatography column, and a second position which reverses direction of fluid flow through the sample chromatography column to deliver the fluidic sample to a sample analyzer. A wash control valve has a first position which supplies a wash buffer solution to the sample chromatography column in a forward fluid flow direction, and a second position which supplies elution solvent to flush the sample supply loop.

The present invention relates to a method of preparing a porous cross-linked polysaccharide chromatography matrix, which comprises to provide an aqueous solution of a gellable polysaccharide, wherein part of the hydroxyl groups are substituted with groups which are not susceptible to nucleophilic attack; to provide essentially spherical droplets of the substituted polysaccharide solution; to form a gel of the substituted polysaccharide solution; and to cross-link the gel by adding a cross-linking agent. The invention also encompasses a chromatography column packed with a matrix so produced as well as use thereof in e.g. protein purification.

The separation of methane from an admixture (110) with ethane and higher hydrocarbons, especially natural gas, using a scrub column (114), in which the admixture is separated into a methane-rich overhead (116) that is partially condensed (122) to provide reflux to the column (114) and liquid methane-depleted bottoms liquid (126), is improved by providing additional reflux (136) derived from an ethane enriched stream (130) from fractionation (128) of the bottoms liquid. Preferably, absorber liquid (140) from the fractionation (128) also is introduced into the scrub column. The vapor fraction (120) remaining after partial condensation can be liquefied (122) to provide LNG product (124).

The invention provides a nitrogen, phosphorus and sulphur doping or co-doping carbon dot and a batch controllable preparing method and application thereof. The method comprises the steps that a carbon source, a nitrogen source, a phosphorus source and a sulphur source are evenly mixed, and a mixture is obtained, wherein the molar ratio of C to N to P to S in the mixture is 1 to 0-0.8 to 0-0.4 to 0-0.4, and the contents of N, P and S are prevented from being zero at the same time; in the air, the mixture is heated to be fused, the reaction is carried out for 3 min to 60 min, natural cooling is carried out till the indoor temperature is reached, a reaction product is separated by a silicagel column, raw materials which do not react are removed, and the nitrogen, phosphorus and sulphur doping or co-doping carbon dot is obtained. According to the method, the technology is simple, the compound time is short, batch producing can be achieved, the doping amount can be adjusted and controlled accurately, the fluorescence color of the prepared carbon dot ranges from blue to green, the application can be achieved on bioluminescence marking and cell imaging aspects, and the good economic benefit and the application prospect are achieved.

Permeable polymeric monolithic materials are prepared in a column casing. In one embodiment, the permeable polymeric monolithic materials are polymerized while pressure is applied through a piston having a smooth piston head in contact with the polymerization mixture. The pressure eliminates wall effect and changes the structure in the column. Similarly, some columns that have a tendency to swell in the presence of aqueous solutions and pressurized while the solution is applied to prevent swelling and wall effect. This procedure also changes the structure in the column. The size of the separation effective openings can be controlled by the amount of the pressure and pores eliminated. Uniformity in the direction flow is improved by controlling polymerization with radiation rather than with conducted heat.

Disclosed herein are a highly purified tocopheryl phosphate and / or a salt thereof (tocopheryl phosphates) wherein a P,P'-bistocopheryl hypophosphate and / or a salt thereof (P,P'-bistocopheryl diphoshates) is contained in a proportion of not higher than 3% by weight; a process for producing a highly purified tocopheryl phosphate and / or a salt thereof, which comprises the steps of reacting a tocopherol with an oxyphosphorus trihalide followed by treating with an acid or basic aqueous solution to thereby form tocopheryl phosphates (i) in which P,P'-bistocopheryl diphoshates (ii) formed as by-products are contained, hydrolyzing the P,P'-bistocopheryl diphoshates (ii) under acid condition, and, optionally, rendering the hydrolyzate neutral or basic under basic condition; and a method of analyzing tocopheryl phosphates, comprising analyzing a sample containing components (i) and (ii) with the use of a high-performance liquid chromatograph column packed with a gel of a polymethacrylate having, bonded thereto, long-chain alkyl groups. None or only an extremely minute amount of P,P'-bistocopheryl diphoshates are contained in the highly purified tocopheryl phosphates, so that the highly purified tocopheryl phosphates exhibit antioxidant and blood circulation promoting effects, have excellent water solubility, are powdery so that the handling thereof is extremely easy, are free from cutaneous irritation and allergenecity and ensure dermal safety. Therefore, the highly purified tocopheryl phosphates are useful as cosmetic ingredients. The amounts of components (i) and (ii) can be simply measured with high accuracy by the above method.

A chromatography and fluidic device with connections capable of automated component changing, diagnostic leak and current sensing. The chromatography-electrospray device contains a chromatography column, a pre-column, a spray emitter, or other fluidic component imbedded within one or more inserts. The inserts are robotically placed in receiving hardware, and a “plug and play” compression fitting connection mechanism makes the fluidic seals in an automated fashion. A plurality of sensors capable of detecting leaks is situated in the device near leak-prone regions. The electrospray emitter has a current sensing electrode in proximity of the electrospray region, capable of detecting the electrospray current. In conjunction with an electronics system, these sensors allow for system and component diagnostics. The diagnostic information may then be used for manual or automated system repair.

The invention discloses a comprehensive extraction method for extracting tea seed oil, tea saponin and tea seed polysaccharide from tea seeds or camellia seeds, belonging to the process fields of grain and oil processing and byproduct extraction. The method comprises the following steps of: with shelled tea seeds as a raw material, firstly, cooking to produce oil; then, adding an exogenous enzyme to obtain a boiled oil layer of a first level an emulsified oil layer, a water layer and a slag layer through zymolysis, blanching and still standing; demulsifying the emulsified oil layer to take boiled oil of a second level; mixing the oil layers of the two levels; centrifugalizing to obtain the tea seed oil; depositing the water layer in alcohol to separate the tea saponin and the tea seed polysaccharide; leaching the slag layer with water or monohydric alcohol to further separate the tea saponin and the tea seed polysaccharide; and purifying a clear solution which is leached with the water or the alcohol with a macroporous absorption resin chromatography column, eluting a resin adsorbate with a base and then an ethanol and collecting an ethanol eluant to obtain high-purity tea saponin. The tea seed oil, the tea saponin and the tea seed polysaccharide are synchronously prepared, thus the invention realizes that a tea seed or camellia seed resource is comprehensively extracted and utilized and has the advantages of simple process, less needed equipment and low cost.

The nature of this invention encompasses the creation of a capillary gas chromatography (GC) column assembly and a thermal modulator used to heat or cool the column assembly in a very thermally and chromatographically efficient manner. The GC column assembly described herein consists of capillary GC column material, such as fused silica or metal capillary tubing, which is constrained to lie in a flat, ordered, spiral pattern and then encased between two thin opposing surfaces. The resulting column assembly is flat, dimensionally stable and can be very efficiently thermally modulated. The resulting column assembly also takes up very little space, has very little thermal mass, and can be easily and accurately manufactured. The column assembly can be adapted for chromatographic use by affixing it to the surface of a thermal modulator described herein by means of adhesive force or by mechanical compression, and then by attaching the free ends of the exposed column material to the input and output ports of the chromatographic device. The temperature of the thermal modulator and capillary column assembly is to be controlled by the chromatographic device or by standalone temperature controlling electronics. The thermal modulator described herein contains an element for temperature modulation of the capillary column assembly to which it is attached and a temperature sensing element for providing accurate temperature feedback to the controlling electronics. The overall result of this when coupled to a chromatographic device is maximally efficient chemical separations in a small space with minimal power consumption.

The invention relates to a method for preparing tobacco extract and application thereof. The method for preparing the tobacco extract uses tobacco as a raw material to prepare the tobacco extract through the steps of enzymolysis, ultrasonic extraction, filtration, chromatography column separation and concentration under reduced pressure; and the tobacco extract is dissolved by 80 percent ethanol and sprayed into tobacco shreds by adopting an atomizing mode. The method using enzymolysis technology and ultrasonic extraction and combining the chromatography separation has the advantages of low extraction temperature, short time, little using amount of a solvent, full extraction and high raw material utilization rate; because the extraction temperature is low, the damage to thermal-sensitive fragrant substances is reduced, fragrant components of the tobacco self is furthest kept, and simultaneously the substances influencing the taste and mouthfeel are removed; and the method is simple, the equipment is easy to operate, and no pollution is produced.

A method separating tocotrienols from tocopherols and the isomers thereof on a large-scale or commercially feasible basis. Separation is accomplished using reverse phase partition liquid chromatography. The chromatography column is prepared using a stationary phase comprising a hydrophobic or aromatic reverse phase chromatography media. The mobile phase consists of a solvent which is capable of solubilizing the crude stock, tocotrienols and tocopherols. The chromatography column containing the stationary and mobile phases is loaded with a crude feed stock and the tocotrienols, tocopherols and / or isomers thereof are eluted at a predetermined linear velocity.

Aspects of the invention provide systems and methods for high-throughput screening of fluidic samples. In some embodiments, two chromatography columns are utilized in series. The first chromatography column can have a high affinity for phosphorylated compounds while the second chromatography column has a high affinity for one or more analytes of interest.

A chromatography stand for supporting a chromatographic column in use for chromatographic analysis has a first and a second platen adapted to receive and hold the chromatographic column therebetween. The first and second platens are mounted on a frame in generally opposed relation for relative movement toward and away from each other. At least one of the first and second platens is constructed for plug-in connection to the chromatographic column such that the column is positively located relative to the platens and placed in fluid connection through said at least one platen. In one embodiment, the cartridges can be stacked between the platens. The cartridges can be quickly and efficiently pre-loaded with sample.

The invention discloses a method for synthesizing nitrogen doped carbon quantum dots based on one-step monocomponent hydro-thermal synthesis, which comprises the following steps: mixing and dissolving ammonium citrate with water, putting the mixture in a sealed reaction vessel, performing heat preservation at 140-220 DEG C for 4-48 hours, after the reaction, naturally cooling to room temperature, and separating the product by a silica gel chromatographic column to obtain the nitrogen doped carbon quantum dots. Compared with existing methods, the method of the invention adopts a single and cheap raw material, is simple in steps, easy to operate, and suitable for mass production.

A multiport, diaphragm sealed valve suitable for use as both a sampling and column switching valve. The valve is constructed to internally block fluid communication between one or more pairs of ports in a valve operating mode. Such blocking may be used to conserve carrier gas when the valve is in the ON position.

A system for high throughput sample preparation and analysis. The system includes a chromatography column including an insoluble matrix. A fluidic circuit is capable of passing a fluid over the insoluble matrix in a first direction such that an analyte in the fluid binds to the insoluble matrix, and back-eluting an elution fluid over the insoluble matrix in a second direction opposite the first direction to output a sample that includes the analyte. A controller controls the fluidic circuit to periodically perform the steps of passing the fluid over the insoluble matrix and back-eluting the elution fluid over the insoluble matrix to output a plurality of samples at a periodic rate.

A non-planar microfabricated gas chromatography column comprises a planar substrate having a plurality of through holes, a top lid and a bottom lid bonded to opposite surfaces of the planar substrate, and inlet and outlet ports for injection of a sample gas and elution of separated analytes. A plurality of such planar substrates can be aligned and stacked to provide a longer column length having a small footprint. Furthermore, two or more separate channels can enable multi-channel or multi-dimensional gas chromatography. The through holes preferably have a circular cross section and can be coated with a stationary phase material or packed with a porous packing material. Importantly, uniform stationary phase coatings can be obtained and band broadening can be minimized with the circular channels. A heating or cooling element can be disposed on at least one of the lids to enable temperature programming of the column.

Nanocolumn chromatography devices useful, e.g., in CEC and nanoLC are disclosed. An exemplary chromatography device of the invention includes a nanocolumn, e.g., a capillary, packed with a particulate stationary phase material and a solid support. The solid support, or in situ frit, is adjacent to and integral with the stationary phase material. An in situ frit of the invention may be a mixture of the stationary phase material and a polymeric network of cross-linked poly(diorganosiloxane), e.g., poly(dimethylsiloxane), which may optionally be sintered. The invention also provides methods of making and using such devices.

An oxygen concentrator comprises an oxygen reservoir and adsorbent columns that each have an inlet, an outlet, and a bed of adsorbent material. The concentrator also comprises an air inlet, an exhaust outlet, and a vacuum pump for pumping nitrogen rich gas from one of the column inlets to the exhaust outlet. A product control pump pumps oxygen rich gas from one of the column outlets to the oxygen reservoir. A control valve controls flow in and out of the columns by selectively connecting the air inlet to one column inlet, connecting the vacuum pump to another column inlet, and connecting the product control pump to a column outlet. A motor drives the vacuum pump and control valve to produce vacuum swing adsorption (VSA) cycles in which oxygen-rich product gas is separated and accumulated in the reservoir.

A process and apparatus for the recovery of ethane, ethylene, propane, propylene, and heavier hydrocarbons from a liquefied natural gas (LNG) stream is disclosed. The LNG feed stream is directed in heat exchanger relation with a warmer distillation stream rising from the fractionation stages of a distillation column, whereby the LNG feed stream is partially heated and the distillation stream is partially condensed. The partially condensed distillation stream is separated to provide volatile residue gas and a reflux stream, whereupon the reflux stream is supplied to the column at a top column feed position. A portion of the partially heated LNG feed stream is supplied to the column at an upper mid-column feed point, and the remaining portion is heated further to partially or totally vaporize it and thereafter supplied to the column at a lower mid-column feed position. The quantities and temperatures of the feeds to the column are effective to maintain the column overhead temperature at a temperature whereby the major portion of the desired components is recovered in the bottom liquid product from the column.

The invention discloses polymorphs A, B, C and D of abiraterone acetate. A preparation method of the polymorphs comprises the step of re-crystallizing the abiraterone acetate subjected to the separation and the purification of a chromatographic column in different solvents. Through stable investigation, four polymorphs have favorable stability and flowability, can be used as raw materials for storage and transportation and are suitably applied to antitumor medicinal preparations.

To make an inexpensive chromatographic column and perform chromatography with it, column walls and a column end with a port are molded integrally from plastic. A closure is integrally molded with a port as well. One type of closure includes part of a snap-on fastener integrally molded to it to cooperate with corresponding parts molded integrally with the column walls. In another type of closure for higher pressures, the closure is spin welded to the tubular walls. In still another type of closure for still higher pressures, a retaining plate is pressed into the column to hold the packing in place. The closures have channels molded into them radiating from a port and opening toward packing material such as silica beads or porous polymeric plugs. Filters and secondary seals may be located at the ends to prevent leakage of the packing material.

The present invention provides a straightforward and robust synthetic process for producing a chromatographic column with eluent-sensitive light diffracting properties based on an inherent photonic band structure and a chromatographic device using the chromatographic column. The present invention provides chromatographic devices employing a chromatographic column which in one embodiment is a photonic colloidal crystal which includes an assembly of colloidal microspheres assembled into a highly ordered array within a housing such as a tube with the highly ordered array being a photonic crystal along the length of the crystal, and a second embodiment which is an inverse construct of the first embodiment, where solid microspheres making up the photonic colloidal crystal chromatographic column are replaced with spherical voids or void spaces subsequent to infiltration of a material of selected refractive index. The photonic band structures of the first type of column made with colloidal particles and the second type of column made by inverting the first type of column may include a photonic band gap, a fundamental stop-band, higher stop-bands, or combinations thereof.

The invention relates to a dissolvent for cellulose dissolution through freezing-unfreezing method or direct dissolution method, and process for preparing the regenerated cellulose fibers and films by utilizing the dissolvent, wherein the dissolvent is the water solution of lithium hydroxide and urea whose compositions are 3.0-7.0wt% of lithium hydroxide, 4.0-30.0.0wt% of urea, the rest is water. The water solution of lithium hydroxide and urea can directly dissolve cellulose through freezing-unfreezing method or by pre-cooling the dissolvent in advance to -10 deg. C - -4 deg. C. It can be used to obtain high dissolvability transparent concentrated cellulose solution. By using the concentrated cellulose solution and through 3-5wt% diluted acid water solution coagulation, regeneration, regenerated cellulose films and fibers can be prepared.