2380 results about "Chromatography column" patented technology

The invention discloses an organic sample analysis method based on micro gas chromatography and a chromatographic analyzer, particularly relates to the technical field of micro gas chromatography, and is used for solving the problems of unstable separation performance and low complex sample detection precision caused by insufficient uniformity of a stationary phase coating of an existing micro chromatographic column. The method comprises the following steps: constructing a stationary phase distribution initial profile by continuously pre-charging carrier gas, alternately applying segmented temperature gradient, micro-negative pressure and directional pulse flow to remodel a uniform distribution layer, generating a correction factor set of a temperature-resistance coupling relationship by combining a correction mixture and a tracer gas pulse, dynamically adjusting a carrier gas flow velocity and a temperature program to generate a matched operation curve, and finally, carrying out dynamic calibration. High-precision separation detection and quantitative analysis are achieved, stable separation performance is maintained in a miniaturized structure by cooperatively controlling stationary phase distribution optimization and real-time parameter feedback correction, and the detection precision and reproducibility of a wide-boiling-range organic mixture are improved.

The invention discloses a method for simultaneously detecting ergothioneine, ergosterol, ergosterol peroxide and lentinine in lentinus edodes, and belongs to the technical field of measurement of active ingredients of the lentinus edodes. The method comprises the following steps: detecting a shiitake mushroom extracting solution by adopting an ultra-high performance liquid chromatography-tandem mass spectrometry mode, wherein a chromatographic column is an HILIC or C18 chromatographic column; the mobile phase A and the mobile phase B are an aqueous solution containing formic acid and a methanol or acetonitrile solution containing formic acid respectively; the mass spectrum conditions comprise an electrospray ionization source; a positive ion multiple reaction monitoring mode; the ion spraying temperature is 315-325 DEG C; the temperature of the ion transmission tube is 320-330 DEG C; and the ion spraying voltage is 3500 V to 3700 V. The method has the characteristics of high sensitivity, high selectivity and high accuracy, and is suitable for quantitative detection of ergothioneine, lentinine, ergosterol peroxide and ergosterol compounds in shiitake mushrooms.

The invention belongs to the field of blood product separation, and discloses a high-efficiency chromatography system and method for blood product separation, and the system comprises an intelligent chromatography column module, an automatic sample injection and accurate flow rate control module, a chromatography filler and column design module, a real-time detection and data analysis module, and an intelligent automatic control and management module. By designing an efficient micro-nano filler chromatographic column and matching with a pulsed electric field auxiliary technology and intelligent automatic control, efficient and high-purity separation and purification of target blood product components are realized. The intelligent regulation and control module used in the invention can dynamically regulate chromatography conditions in real time, and the problems of unstable flow velocity and column pressure in the traditional method are thoroughly solved. And meanwhile, a multi-point real-time detection and feedback technology is adopted, so that the separation process is more accurate, transparent and controllable.

The invention discloses an on-load tap-changer oil dissolved gas monitoring device which comprises a variable capacity container, a two-way electromagnetic valve, a switching mechanism, a chromatographic column system, a high-sensitivity detector and a low-sensitivity detector which are sequentially connected, and the top and the bottom of the variable capacity container are connected through a circulating pump to form a gas flow loop. The variable-capacity container is connected with the on-load tap-changer through a connecting pipe, the switching mechanism is connected with at least two quantitative pipes with different capacities, and the switching mechanism is used for equally transferring gas in the variable-capacity container to the quantitative pipes and then sequentially transferring the gas in the quantitative pipes to the chromatographic column system. The invention further discloses a method for monitoring the dissolved gas in the on-load tap-changer oil. According to the monitoring device and the monitoring method for the dissolved gas in the oil of the on-load tap-changer, the detection range of the concentration is improved, a concentration blind area which is not detected and misjudgment of signal saturation can be avoided, and the detection accuracy is improved.

The present disclosure generally relates to a chromatography column adapter for forming a fluid connection between a chromatography column and a GC component. The present disclosure also relates to a fluid connection for a gas chromatography system.

The present invention discloses a method for separating and purifying recombinant human collagen, wherein the method first filters the recombinant human collagen fermentation broth, then uses cationic composite chromatography to crudely purify the recombinant human collagen to obtain a cationic composite chromatography eluent, and then uses a hydrophobic chromatography column to purify the purified recombinant human collagen, wherein the cationic composite chromatography column is filled with a high-flow rate agarose matrix containing a hydrophobic group of a phenyl group and an electrostatic group of a carboxyl group as a filler, and the hydrophobic chromatography column is filled with a hydrophilic polymethacrylate matrix containing a phenyl group as a filler. The final target protein purity of the present invention is greater than 99%, the recovery rate is greater than 85%, and the endotoxin content is less than 0.5EU / mg. The process of the present invention is simple and easy to operate, and the purification cost requirement is low. The recombinant human collagen obtained by the method has high purity and low endotoxin content, and meets the requirements of cosmetic raw materials, medical devices, and medical-grade high-purity collagen.

The invention provides a method for detecting the concentration of phosphite ions in a natural water medium, which comprises the following steps: detecting a to-be-detected sample solution containing phosphite ions and impurity ions by using an ion chromatograph to obtain a chromatogram of the to-be-detected sample; determining a peak area value of phosphite ions from the chromatogram of the sample to be detected; obtaining the concentration of the phosphite ions in the sample to be detected according to a linear regression equation of the peak area value of the phosphite ions and the concentration of the phosphite ions; wherein the test conditions of the ion chromatograph are as follows: an anion exchange column is selected as a chromatographic column, and the column temperature is 43-56 DEG C; the leacheate is a potassium hydroxide solution, the flow rate is 1.0-2.0 mL / min, gradient leaching is adopted, and the concentration of the leacheate is 15-18 mM within the time period of 0-14 min; the concentration of the leacheate is 33-36 mM within the time period of 16-30 min; the concentration of the leacheate is 17 to 20 mM within the time period of 32 to 36 minutes.

The invention discloses a non-derivatization quantitative detection method for amino acid in a high-concentration protein preparation, which comprises the following steps: separating by using a graphitized carbon chromatographic column, adding an ion pair reagent trifluoroacetic acid into a mobile phase, and detecting by using an electric fog detector (CAD); the method comprises the following steps: preparing a plurality of groups of amino acid standard substance solutions with different concentration groups, wherein the concentration range is 1mmol / L-20mmol / L; drawing a quadratic equation standard curve of the amino acid standard product concentration and the peak area as a detection equation for detecting the amino acid concentration; the method comprises the following steps: diluting a test sample by using ultrapure water for multiple times, carrying out ultrafiltration centrifugal treatment, removing protein, introducing the sample, obtaining a corresponding peak area according to the spectrum integral of the test sample, and substituting the peak area into the detection equation to obtain the concentration of amino acid in the test sample. The method has the advantages of high detection precision, high detection efficiency and simple operation, and greatly reduces the adverse effect of high-concentration protein on the chromatographic column.

The invention discloses a liquid chromatography-mass spectrometry data peak shape recognition processing method and related equipment, and relates to the field of data processing. The method comprises the following steps: acquiring liquid chromatography-mass spectrometry data; extracting morphological characteristic data of a chromatographic peak based on the extracted ion current chromatogram time sequence; calculating and extracting a signal complexity index of the ion current chromatogram time sequence; adjusting peak shape identification parameters according to the signal complexity index, wherein the peak shape identification parameters comprise a noise threshold value and a peak width range; distinguishing the chromatographic peak into a normal peak or an abnormal peak by using the adjusted peak shape identification parameter; searching a liquid phase method optimization scheme matched with the morphological characteristic data of the abnormal peak in a preset optimization database, wherein the liquid phase method optimization scheme comprises gradient elution time adjustment, mobile phase proportion optimization or chromatographic column replacement suggestion; and sending the liquid phase method optimization scheme to a user. According to the invention, various abnormal peak shapes can be automatically and accurately identified, and the reliability and accuracy of identification are guaranteed.

The invention relates to the technical field of liquid chromatogram data processing, in particular to a liquid chromatograph state detection and maintenance method which comprises the following steps: establishing a multi-parameter online monitoring system which comprises a normal data set and an abnormal data set; learning the normal data set and the abnormal data set, and collecting real-time monitoring data to be matched with a known fault mode; according to the real-time monitoring data, the control unit draws a pressure curve and calculates a pressure fluctuation value; determining the test period of the chromatographic instrument based on the pressure fluctuation value and the baseline noise peak value, or sending an equipment fluctuation monitoring signal and calculating the difference value between the chromatographic column temperature and the environment temperature: determining the heating power of a constant-temperature box based on the difference value between the chromatographic column temperature and the environment temperature, or sending an equipment fluctuation monitoring signal; and determining the temperature correction coefficient of the chromatographic column according to the equipment vibration frequency. According to the invention, the operation stability and effectiveness of the liquid chromatograph are improved.

The invention discloses a fruit and vegetable exosome extraction method based on ligand fishing. The fruit and vegetable exosome extraction method comprises the following steps: suspending amino-functionalized Fe3O4 (at) SiO2 nanoparticles in a glutaraldehyde solution, and adding activated agglutinin to obtain Fe3O4 (at) SiO2-agglutinin nanoparticles; adding a glutaraldehyde solution into agarose gel to obtain a size exclusion chromatographic column filler; the method comprises the following steps: dispersing Fe3O4 (at) SiO2-lectin nanoparticles in a PBS buffer solution to obtain a uniform suspension, adding the uniform suspension into a size exclusion chromatographic column filler to obtain a uniform slurry, filling the size exclusion chromatographic column with the uniform slurry, and flushing with the buffer solution to form a magnetic bead stationary phase; and loading fruit and vegetable supernatant into a size exclusion chromatographic column, passing through the magnetic bead stationary phase, cleaning residual impurities combined on the exosome-magnetic bead compound by using a buffer solution, and dissociating the exosome from the magnetic beads by using a competitive eluent of the exosome to obtain the high-purity fruit and vegetable exosome. The problem that an existing exosome extraction method is difficult to meet the requirements of high efficiency, simplicity, high purity and high activity at the same time is solved.

The utility model relates to the technical field of ion chromatographs, in particular to a column oven of an ion chromatograph. A column oven of an ion chromatograph comprises a column oven structure and a column oven isothermal body arranged in the column oven structure, a chromatographic column and a temperature control assembly are arranged on the column oven isothermal body, a heat insulation structure matched with the column oven isothermal body is arranged in the column oven structure, and a valve assembly matched with the chromatographic column to form a passage is further arranged in the column oven structure. According to the utility model, by controlling and monitoring the temperature in the column box structure, the temperature can be accurately controlled and maintained in a proper range, so that the temperatures of the chromatographic column, the environment, the liquid and the like are consistent, and the reliability and the accuracy of an analysis test are ensured.

The invention belongs to the field of pharmaceutical analysis, and discloses a method for simultaneously determining the contents of tolperidone hydrochloride, methyl hesperidin and vitamin B6 in Naoluotong capsules, the method comprises three steps of preparation of a mixed reference solution, preparation of a test solution and content determination, and the chromatographic conditions of the content determination are as follows: a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column and a C18 chromatographic column; a mobile phase is methanol (A)-0.1% glacial acetic acid solution (B), gradient elution is carried out for 0-5 min, and 96% of B is obtained; 5-6 min, 96%-60% of B is selected; 6-20 min, the content of B is 60%-50%; the volume flow rate is 1.0 ml / min; the column temperature is 30 DEG C; the detection wavelength is 291 nm; the sample size is 1 microliter. According to the method, synchronous detection of three components (tolperidone hydrochloride, methyl hesperidin and vitamin B6) with remarkable physicochemical property difference can be realized, the chromatographic condition is simple, and the detection speed is high.

The invention belongs to the field of detection and analysis, and relates to a method for separating and detecting enantiomers in (S)-2-amino-3-[2-(tert-butoxy)-2-oxoacetamido] tert-butyl propionate hydrochloride. According to the method, HPLC is adopted for separation and detection, a polysaccharide derivative chiral chromatographic column is selected as a chromatographic column, a mobile phase A is a weak base aqueous solution, and a mobile phase B is acetonitrile and / or methanol. The method is strong in specificity, high in separation degree, high in accuracy, stable, reliable and high in sensitivity, and a simple, accurate, rapid and reliable detection method is provided for control of impurities in a starting material of (S)-2-amino-3-[2-(tert-butoxy)-2-oxoacetamido] tert-butyl propionate hydrochloride.

The invention discloses a construction method and an identification method of characteristic chromatograms of a senecio scandens medicinal material as well as decoction pieces, standard decoction and formula granules thereof, and relates to the technical field of traditional Chinese medicine identification. The construction method of the specific chromatogram comprises the following steps: extracting a to-be-detected sample with methanol to obtain a test solution; the method comprises the following steps: dissolving 5-caffeoylquinic acid, chlorogenic acid, caffeic acid, 4-caffeoylquinic acid, rutin, hyperoside, isoquercitrin and 4, 5-dicaffeoylquinic acid with methanol to obtain a reference solution; and a liquid chromatograph is adopted for determination. A chromatographic column of the liquid chromatograph takes phenyl-methyl polysiloxane as a stationary phase, acetonitrile as a mobile phase A and 0.05 vol%-0.3 vol% formic acid aqueous solution as a mobile phase B for gradient elution. The information of the characteristic chromatogram constructed by the method is rich, and the quality of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules can be quickly and comprehensively reflected.

The invention relates to the technical field of chromatographic detection and material characteristic analysis, and discloses a multiphase chromatographic analysis system and method.The multiphase chromatographic analysis system comprises a multiphase sample injection module, a multi-field cooperative regulation and control separation column module and a plurality of separation chromatographic columns which are sequentially connected, and the separation chromatographic columns comprise partitions and are installed in a column box in an end-to-end connection mode; a temperature gradient field, an electric field gradient field, a controllable electromagnetic field and a fluid power field; and a multi-modal detection module, a data acquisition and control module and an auxiliary module. Through the brand-new multi-phase sample injection module, the multi-field coordinated regulation and control separation column module and the multi-mode detection module, direct input of detected objects in different phase states can be met, and phase state conversion is carried out according to needs, so that the phase state compatibility of the analysis system to the detected objects is obviously enhanced; and different components can be obviously separated according to different boiling points, migration speeds and magnetic susceptibilities by combining multi-field synergistic separation, so that the chromatogram is more accurate.

The invention belongs to the field of egg by-product processing, and particularly relates to a preparation method and application of eggshell membrane peptides, the preparation method of the eggshell membrane peptides comprises the following steps: S1, drying and crushing cleaned eggshells, and then separating eggshell membranes; s2, putting the eggshell membrane into a steam explosion machine, carrying out pressure maintaining treatment for 1-5 minutes under the pressure of 1.0-1.5 MPa, then instantly releasing the pressure, and collecting a steam explosion sample; s3, adding the eggshell membrane subjected to steam explosion treatment into a sodium hydroxide aqueous solution with the pH value of 7-9 according to the solid-liquid ratio of 1: (20-30), then adding alkaline protease according to the enzyme addition amount of 5000-15000U / g, carrying out enzymolysis for 5-10 hours at the temperature of 35-65 DEG C, then inactivating for 3-5 minutes at the temperature of 100 DEG C, filtering enzymatic hydrolysate, taking filtrate, centrifuging, taking supernate, and freeze-drying to obtain polypeptide; s4, polypeptide obtained in S3 is dissolved in an activation solution to be activated for 8-10 h at the temperature of 4-8 DEG C; and S5, separating the sample activated in the step S4 through a Sephadex G-15 chromatographic column to obtain the activated eggshell membrane peptide. Based on the method, the preparation efficiency is high, and the prepared eggshell membrane peptide is high in antioxidant activity.

The invention provides a traditional Chinese medicine extract component detection method and system, and relates to the field of traditional Chinese medicine detection. The component detection system for the traditional Chinese medicine extract comprises a sample preparation module used for pretreatment of dissolution and dilution of a sample and treating the traditional Chinese medicine extract sample into a solution state suitable for chromatographic analysis; the multi-dimensional chromatographic separation module comprises a first-dimensional chromatographic column, a second-dimensional chromatographic column, a pump and a sample injector, and is used for effectively separating different components in the complex traditional Chinese medicine extract sample so as to facilitate subsequent detection and analysis. The separation degree of a complex traditional Chinese medicine extract sample can be effectively improved by utilizing the selectivity difference of different chromatographic columns through a multi-dimensional chromatographic separation technology, meanwhile, the analysis time can be shortened and the analysis efficiency can be improved by optimizing the mobile phase composition and the gradient elution procedure, the sample loss can be reduced through multi-dimensional chromatographic separation, and the detection accuracy is improved. And the detection sensitivity and accuracy are improved.

The invention relates to a chromatographic instrument temperature control method and system, an intelligent terminal and a storage medium, and relates to the technical field of chromatographic instrument sample separation.The method comprises the steps that the sample type of a preset sample is obtained; analyzing the sample type to determine a window heating rate and a window target temperature; acquiring a chromatographic column initial temperature of a preset chromatographic column; analyzing the initial temperature of the chromatographic column and the target temperature of the window to determine the temperature of the chromatographic column needing to be increased; analyzing the temperature required to be increased and the window heating rate of the chromatographic column so as to determine the heating time of the chromatographic column; and controlling a preset chromatographic column temperature regulation and control device to perform temperature control on the chromatographic column according to the chromatographic column heating time and the window target temperature. The method has the effect of improving the precision of the temperature retention time.

The utility model discloses a chromatographic column filtering device, which relates to the technical field of installation equipment and comprises a chromatographic column main body and a liquid inlet fixed on one side of the chromatographic column main body. Through the arrangement of the vibration mechanism arranged on the back of the filter screen and the cleaning mechanism arranged in the motor box, when the surface of the filter screen is cleaned, the servo motor on the back of the filter screen is started, the servo motor drives the eccentric wheel on one side to rotate, and the eccentric wheel is influenced by centripetal force to drive the servo motor to vibrate; and secondly, a driving motor in a motor box on one side is matched, the driving motor drives a brush head to sweep the surface of the filter screen, and the impurities on the surface of the filter screen are swept down. The filter screen is cleaned in a vibrating and sweeping mode, and the service life of the filter screen is prolonged.

The invention relates to a method for detecting and / or purifying a protein polymer and application thereof. The method comprises a step of detecting the protein polymer based on HPLC-SEC. The chromatographic conditions are as follows: a chromatographic column is a molecular size exclusion high performance liquid chromatographic column; and a PB buffer solution with the concentration of 50-200mM is used as a mobile phase. The method disclosed by the invention is good in separation degree, simple to operate, short in peak appearance time and short in detection time, and has good method reproducibility and stability.

The invention relates to the technical field of immobilized lipase, in particular to immobilized lipase taking modified nano SiO2 as a carrier as well as a preparation method and application of the immobilized lipase. The immobilized lipase sold in the market is high in price and poor in mechanical strength, the enzyme loss is too large due to shear force or carrier structure damage in the continuous production process, and the transesterification efficiency is poor. In order to solve the technical problems, the invention provides the immobilized lipase taking the modified nano SiO2 as the carrier, the immobilized lipase is obtained by sequentially grafting a modified nano silicon dioxide carrier with an amino silane coupling agent and an epoxy silane coupling agent and loading lipase on the surface of the modified nano silicon dioxide carrier, according to the method, the immobilized lipase forms an enzyme column in a chromatographic column and then serves as a reactor, damage of mechanical stirring to lipase particles is avoided, the ester exchange degree is more effectively controlled by adjusting the flow speed of a substrate, the production cost of an enterprise can be remarkably reduced, and the product quality is improved.

The invention relates to a hydrophilic core-shell type chromatographic stationary phase material and application thereof. The hydrophilic core-shell type chromatographic stationary phase material comprises spherical silicon dioxide and a mesoporous layer which coats the spherical silicon dioxide, contains an amide group and is compounded with silicon dioxide. The preparation method comprises the following steps: firstly preparing solid silicon dioxide microspheres, then coating the surfaces of the silicon dioxide microspheres with mesoporous silicon dioxide shell layers through surface in-situ growth, and finally modifying the mesoporous shell layers through grafting and polymerization reaction to obtain the hydrophilic core-shell material containing the amide groups. The aperture of the material is mesoporous 2-20 nanometers, hydrophilic oligosaccharide substances can be separated, radix morindae officinalis oligosaccharide can be separated, and compared with a traditional chromatographic column, the separation degree is better, the column efficiency is higher, and the good separation performance is achieved. The preparation steps are simple, the reaction conditions are mild, and the method is simple.

The invention discloses a method for rapidly determining the content of breast milk oligosaccharide in dairy products, which comprises the following steps: sampling and pretreating different types of dairy products according to sample states, carrying out ultrasonic extraction, refrigerated centrifugal degreasing and centrifugal ultrafiltration protein purification on the obtained sample, and reducing the purified sample solution by using sodium borohydride to obtain a sample solution to be detected; reducing the breast milk oligosaccharide standard substance by sodium borohydride to obtain a standard solution to be detected; determining the standard substance solution and the to-be-detected sample solution by adopting ultra-high performance liquid chromatography-tandem mass spectrometry, establishing a standard curve, and carrying out quantitative analysis on the to-be-detected sample solution; the chromatographic conditions are as follows: an ACQUITY UPLC BEH Amide hydrophilic interaction chromatographic column is adopted; the mobile phase A is 20 mmol / L ammonium formate; the mobile phase B is acetonitrile; and gradient elution.

The invention relates to the technical field of lubricating oil analysis, in particular to a method and system for quantitative analysis of a lubricating oil additive. The method comprises the following steps: acquiring base oil data and lubricating oil additive data; performing mixed oil treatment based on the base oil data and the lubricating oil additive data to generate mixed lubricating oil additive data; performing mixed oil concentration detection on the mixed lubricating oil additive data to generate mixed oil concentration detection data; performing chromatographic column selection on the mixed lubricating oil additive data based on the mixed oil concentration detection data to generate chromatographic column selection data; and performing boiling point heating on the chromatographic column selection data to obtain mixed oil boiling point heating data. By optimizing data acquisition, chromatographic separation, component identification and quantitative analysis as well as result analysis and visualization, the accuracy of quantitative analysis of the lubricating oil additive is improved.

The invention relates to a chromatographic device, a chromatographic system and a chromatographic column replacement method, the chromatographic device comprises a chromatographic box and a quick-insertion quick-change mechanism, the chromatographic box comprises a box body and a chromatographic column arranged in the box body, the two ends of the chromatographic column are connected with an adapter assembly penetrating through the side wall of the box body, the adapter assembly is provided with a first channel communicated with the chromatographic column, and the first channel is communicated with the chromatographic column. The quick-inserting and quick-changing mechanism comprises a support and a sample feeding and discharging unit arranged on the support, the support is provided with a positioning assembly used for positioning the chromatographic box, the sample feeding and discharging unit comprises two movable sample needles, the two sample needles are used for being connected with the two switching assemblies respectively, and when the chromatographic box is positioned on the support, the two switching assemblies are connected with the two movable sample needles. The sample needle can move between a first position and a second position; when the sample needle moves to the first position, the sample needle is inserted into the first channel of the switching assembly, so that the sample needle is communicated with the first channel; and when the sample needle moves to the second position, the sample needle is separated from the switching assembly. According to the invention, the chromatographic column can be rapidly replaced from the chromatographic device in a mode of replacing the chromatographic box.

The invention discloses a method for detecting an intermediate acetylglucose of tetraacetyl by an HPLC-CAD (High Performance Liquid Chromatography-Computer Aided Design) method. The method comprises the following steps: (1) preparing a reference substance solution, namely dissolving acetylglucose by adding a diluent, and diluting to prepare the reference substance solution; (2) preparation of a test solution: taking tetraacetyl, adding a diluent solvent, and diluting to prepare the test solution; and (3) detecting the content of acetyl glucose in the test sample by adopting an HPLC-CAD (High Performance Liquid Chromatography-Computer Aided Design) method, and taking octadecylsilane chemically bonded silica as a filler in an adopted chromatographic column. According to the method disclosed by the invention, the intermediate acetyl glucose of the tetraacetyl substance is detected based on an HPLC-CAD (High Performance Liquid Chromatography-Computer Aided Design) method, and CAD parameters, a chromatographic column, a mobile phase, a diluent and a sample concentration are selected, so that the aims of effectively separating and accurately detecting the acetyl glucose in the sample can be fulfilled; the method has the advantages of simplicity, sensitivity, high accuracy, high repeatability, high specificity, good durability, high precision and the like, and has certain guiding significance on carbohydrate compound detection.

The present invention discloses a method for alcoholysis of EVA, which relates to the technical field of EVA. The technical solution is as follows: S1 Soak the alkaline anion exchange resin in deionized water for swelling; S2 Wash the alkaline anion exchange resin in deionized water; S3 Pack the washed alkaline anion exchange resin in a chromatography column and wash it with a hydrochloric acid solution; S4 Wash the eluted alkaline anion exchange resin with deionized water until neutral, and then wash it with a sodium hydroxide solution to obtain the activated alkaline anion exchange resin; S5 Pass the EVA solution through the activated alkaline anion exchange resin chromatography column to obtain crude EVOH; S6 The crude EVOH after alcoholysis is washed with water and dried under vacuum, then enters the extrusion equipment, and is granulated and washed to obtain EVOH resin. The present invention solves the problems of introducing metal impurities by the NaOH catalyst, complex post-treatment process and environmental pollution, and reduces the preparation difficulty and cost of EVOH.

The invention discloses a tetanus toxoid refining method which comprises the following steps: S1, centrifuging tetanus fermentation harvest liquid by adopting a butterfly centrifuge to remove thalli, and then performing ultrafiltration concentration to obtain a concentrated solution; s2, treating the concentrated solution obtained in the step S1 with a D390 ion exchange chromatographic column to obtain a chromatographic solution; s3, treating the chromatographic solution obtained in the step S2 by virtue of a Superdex 200 ion exchange chromatographic column, eluting by virtue of a sodium carbonate buffer solution, and collecting an elution peak, namely target protein, so as to obtain an eluent; and S4, performing formaldehyde detoxification treatment on the eluent obtained in the step S3, performing ultrafiltration to remove formaldehyde, and finally performing sterilization through a filter membrane to obtain the toxoid stock solution. In the refining process, the loss of the target protein is less, and the prepared toxoid is relatively excellent in immune effect and relatively high in purity.

The invention discloses a gas chromatographic analysis device and a gas chromatographic analysis method, and relates to the technical field of detection and analysis. The gas chromatographic analysis device comprises a sample injector, a column oven and a detector. The sample injector comprises a plurality of sample injection pipelines; a plurality of chromatographic columns and a conduction device are arranged in the column oven, each chromatographic column is provided with a sample inlet end and a sample outlet end which are oppositely arranged, and the plurality of sample inlet ends are correspondingly communicated with the plurality of sample inlet pipelines; the conduction device comprises a plurality of input ends and an output end, and the plurality of input ends are correspondingly communicated with the plurality of sample outlet ends; the detector is communicated with the output end; the plurality of chromatographic columns are arranged to be chromatographic columns with at least two polarities. Continuous detection and analysis can be realized only by selecting the corresponding sample inlet, so that the long-term use and maintenance cost is reduced, and the risk of simultaneous sample injection of a plurality of chromatographic columns due to improper valve switching is reduced.