237 results about "Chromatography column" patented technology

The present application is applicable to the field of medical devices, and discloses a liquid chromatography-mass spectrometry apparatus and a control method for the liquid chromatography-mass spectrometry apparatus. The liquid chromatography-mass spectrometry apparatus comprises a sample storage device, a pretreatment device, a chromatography device, a mass spectrometry detection device, and a controller. The chromatography device comprises a test solution preparation channel, a test solution transfer assembly, a liquid phase fluid delivery assembly, and a chromatography column. An operating pressure outputted by the liquid phase fluid delivery assembly is less than or equal to 20 MPa. The length of the chromatography column is greater than or equal to 3 mm and less than or equal to 35 mm. A sample comprises a target substance to be measured, and the target substance to be measured comprises at least one of vitamin D, a steroid hormone, and a catecholamine. The present application reduces costs of the liquid chromatography-mass spectrometry apparatus.

The application provides a biological analysis method capable of accurately, simply and quickly detecting the concentration of adenosylcobalamin in human serum. The method is a liquid chromatography tandem mass spectrometry method, octadecylsilane bonded silica gel is used as the column filler, the liquid phase conditions, mass spectrometry parameters and sample processing method are designed and optimized, and the application first proposes and discloses a biological analysis method capable of accurately and quickly detecting the concentration of adenosylcobalamin in human serum: after simple treatment (need to avoid light) such as protein precipitation and nitrogen blowing dry, the sample can be injected, the analysis time is only 4.00 min, the lower limit of quantification is as low as 5 pg / mL, and the sample dosage is only 100 muL. The application can quickly monitor the change of vitamin B12 in the body, meet the requirements of adenosylcobalamin in vivo drug monitoring, pharmacokinetics and bioequivalence, and can effectively improve the safety and reliability of the drug.

The application discloses a method for simultaneously determining phenolic acid and tanshinone components in Danshen injection, and according to the structure and property characteristics of the phenolic acid and tanshinone components, the best mobile phase composition, elution program, flow rate, chromatographic column and mass spectrometry condition are screened through a large number of experiments. Experimental verification shows that the UPLC-TQ-MS / MS method can simultaneously determine 11 different structure and type of phenolic acid and tanshinone component compounds, and the methodological verification shows that the detection method has good precision, repeatability and high accuracy, and has important significance for controlling the quality of Danshen injection and guaranteeing the clinical curative effect.

This utility model relates to a column housing device for housing a chromatographic column within a column oven. The device includes: a housing with a base having a first side and an opposite second side, wherein the first side has a groove; a first sidewall and a second sidewall extending from the first side of the base, wherein the second sidewall is arranged opposite to the first sidewall; and a support member including a bracket disposed in the groove, such that the support member is detachably fixed to the first side of the base via the bracket between the first and second sidewalls, thereby forming a heat exchange channel between the base, the support member, and the first and / or second sidewalls for placing the chromatographic column. This column housing device detachably connects the support member to the housing, providing a flexible and adjustable thermal structure that balances column placement stability and heat transfer efficiency. This utility model also relates to a column oven and a chromatogram.

The utility model discloses a kind of device for separating and purifying cosmetic functional raw materials using affinity chromatography technology, it is related to chromatography column field, including fixed plate, chromatography column, heat preservation shell and connecting assembly, chromatography column is by effluent bucket, glass shell and top cover composition, fixed plate fixedly sets in effluent bucket outer wall, effluent bucket and top cover are with glass shell and are formed sealed cavity by thread connection, heat preservation shell is clamped by two half shells and is wrapped in chromatography column outer wall, connecting assembly is installed in fixed plate lower surface, connecting assembly is used for the fixation and separation of two half shells, the utility model is split by heat preservation shell, so that scale can be completely removed in cleaning process, improve heat transfer efficiency, ensure the uniformity of chromatography column internal temperature distribution, conducive to maintaining the stability and separation effect of chromatographic process.

This invention provides a 210 The purification method for Po includes: under vacuum, distilling irradiation of a thorium oxide target to generate distilled gas, condensing the distilled gas in a collecting pan to obtain a distillate; soaking the collecting pan in nitric acid solution to obtain a soaking solution, evaporating it to obtain a dried product, dissolving the dried product in hydrochloric acid solution to obtain a leachate; pouring the leachate into a chromatography column packed with chloride-form anion exchange resin, rinsing the chromatography column with hydrochloric acid solution, and draining the liquid; rinsing the chromatography column with hydrochloric acid solution to allow the chloride-form anion exchange resin to react with the thorium oxide. 210 Po separation yields a product containing... 210 The purified solution of Po was diluted to obtain a diluent. Tetravalent tellurium, a reducing agent, and isopropanol were added to the diluent to obtain a mixture. The mixture was filtered to obtain an α-measurement source, and the α-spectrum was obtained by measurement. The purification method of this embodiment utilizes a thorium oxide target obtained after irradiation. 210 The differences in physicochemical properties between Po and impurities enable... 210 Purification of Po.

This invention relates to the field of medical devices and discloses a liquid chromatography-mass spectrometry (LC-MS) device and a control method for the LC-MS device. The LC-MS device includes a sample storage device, a pretreatment device, a chromatographic element, a mass spectrometry detection device, and a controller. The chromatographic element includes a reagent preparation channel, a reagent transfer assembly, a liquid flow delivery assembly, and a chromatographic column. The liquid flow delivery assembly is used to drive the reagent in the reagent preparation channel through the chromatographic column to adsorb the reagent onto the column, and to drive the liquid flow through the chromatographic column to elute the adsorbed reagent to form the analyte. The pretreatment device uses magnetic separation to pretreatment the sample. The operating pressure of the liquid flow delivery assembly is less than or equal to 20 MPa. This invention reduces the cost of LC-MS devices.

The application provides a detection method of hydroxypropyl cellulose in a povidone-containing preparation, and specifically comprises the following steps: (1) preparing a sample solution by pretreating a sample to be detected; (2) detecting the sample solution by using HPLC, wherein an evaporative light scattering detector or an electrospray detector is used as a detector, a C18 chromatographic column is used as a chromatographic column, the column temperature is 20-40 DEG C, water is used as a mobile phase A, methanol is used as a mobile phase B, the flow rate is 0.5 ml / min-1.5 ml / min, and gradient elution is adopted. The detection method has good specificity and peak type, can not only separate hydroxypropyl cellulose from povidone and other high-molecular auxiliary materials in a pharmaceutical preparation, accurately quantify the content of hydroxypropyl cellulose, but also effectively identify the model, and provides a good method for reverse analysis, component analysis, auxiliary material release detection of the pharmaceutical preparation.

Embodiments of the present disclosure describe a system and method for controlling the pH of a polypeptide-containing mixture. The pH may be controlled for any suitable purpose, for example, to inactivate a virus in the mixture. The method may include the step of eluting a mixture (e.g., an eluent) having a pH greater than, for example, 3.9 and, for example, less than, 8.5 from a chromatography column. The method may further include one or more of the steps of measuring the protein concentration of the mixture and measuring the pH of the mixture. Subsequently, the amount of acid required to reduce the pH of the mixture to a target pH may be calculated based on the protein concentration of the mixture, the pH of the mixture, or both. After calculating the amount of acid to be added, a portion of the acid may be added to the mixture, wherein said portion of the acid is sufficient to achieve the target pH.

The utility model discloses a column temperature box with automatic chromatographic column selection system, including the box, the box surface is equipped with the door body of rotation connection, is equipped with the valve cut system in the box to the door body one side, and the valve cut system includes the mounting panel in the box top, the chromatographic column installation valve head on the mounting panel, the control valve in the chromatographic column installation valve head tail portion, is equipped with the temperature control area below the valve cut system, and the temperature control baffle that installs in the box is equipped in the temperature control area, and the mounting bracket on the surface of temperature control baffle and the chromatographic column on the mounting bracket, the bottom of temperature control area still is equipped with the liquid leakage device.

The application discloses a capillary open-tube chromatographic column preparation method based on a defect engineering metal organic framework-molecular imprinting composite nanomaterial and application, and particularly relates to the field of chemical synthesis and analysis technology. A GLYMO-IDA-silane solution is used to flush a pretreated capillary, so that a carboxyl-modified capillary is obtained. Then, an N,N-dimethylformamide solution containing zirconyl chloride octahydrate, terephthalic acid, L-proline and hydrochloric acid is injected into the cysteine-modified capillary, and under a heating condition, a metal organic framework UiO-66-L-Pro is in-situ grown on the inner wall of the capillary. D-tryptophan and norepinephrine are dissolved in a neutral buffer solution, and after magnetic stirring for 30 min, ferrous sulfate is added and rapidly injected into the UiO-66-L-Pro-modified capillary, and then both ends are sealed, and reaction is carried out at room temperature for 24 h. An acetic acid / SDS solution mixture is used to flush the capillary for 1.5 h to elute the template molecules, and then nitrogen is used to blow dry, so that an MIP@UiO-66-L-Pro@capillary is prepared.

The utility model discloses a separation and purification device based on macroporous resin, and relates to the technical field of separation and purification, the separation and purification device based on macroporous resin comprises a chromatographic column, the chromatographic column is provided with a chromatographic structure convenient for separation and purification, the chromatographic structure comprises a feed port, a fixed base and a circular truncated cone flow guide ring, the feeding hole is fixedly formed in the upper end part of the chromatographic column, the fixed base is positioned at the lower end part of the chromatographic column, each circular truncated cone flow guide ring is positioned on the inner side wall of the chromatographic column, an adjusting valve is arranged at the lower end part of the chromatographic column, and two column mounting grooves are formed in the upper end part of the feeding hole. The chromatographic column is arranged to purify anthocyanin, the purity of the obtained anthocyanin is higher than that of unpurified anthocyanin, toxic organic solvents are avoided in the whole process, only ethyl alcohol is used, environment friendliness is achieved, resin can be repeatedly used, and the production cost is reduced.

FIELD: pharmacology.SUBSTANCE invention can be used to determine the concentration of an anti-tuberculosis drug in blood serum. The method for determining the concentration of bedaquiline in the blood serum in patients with tuberculosis or mycobacteriosis is that whole blood samples are centrifuged at 3000 rpm for 20 minutes, then the blood plasma is collected in sterile 1.5 mL Eppendorf tubes, then 900 mcL of acetonitrile are added to 300 mcL of plasma and centrifuged at 13500g for 15 minutes, then 1 mL of the supernatant is transferred to a chromatographic vial and make the assay by UHPLC MS / MS using an Athena UHPLC C18, 1.8 mcM, 120A, 2.1×100 mm chromatography column, when using an aqueous solution containing 5 g / L of ammonium acetate, 25 ml / L of concentrated acetic acid, 2 ml / L trifluoroacetic acid as mobile phase A and 100% acetonitrile as mobile phase B, the concentration of bedaquiline is determined using a pre-plotted calibration curve.EFFECT: determination of bedaquiline in human blood plasma in a time not exceeding 6 minutes.1 cl, 9 dwg, 6 tbl

The utility model discloses a trace ion determination device in pure water, which comprises: a first multi-way switching valve, a quantitative ring, a sample injection needle and a chromatographic column are arranged on the first multi-way switching valve; a second multi-way switching valve, a concentration column and a first chromatographic pump are arranged on the first multi-way switching valve; the second multi-way switching valve is further connected with the first multi-way switching valve; a third multi-way switching valve, a second chromatographic pump and a cleaning container are arranged on the third multi-way switching valve; the third multi-way switching valve is further connected with the second multi-way switching valve; a fourth multi-way switching valve, the fourth multi-way switching valve is connected with the second multi-way switching valve and the third multi-way switching valve respectively; the fourth multi-way switching valve is configured to switch the liquid flow direction of the sample injection needle or the cleaning container output to the concentration column or a waste liquid container; a purifier is further arranged between the fourth multi-way switching valve and the third multi-way switching valve. The requirements of trace detection in pure water are met.

The present disclosure relates to methods of characterizing a system comprising a chromatography column. The methods can comprise introducing a sample comprising a positive control and a negative control into a system comprising a chromatography column, wherein the positive control is a sensitive probe that interacts with the system and the negative control does not substantially interact with the system; detecting the positive control and the negative control after passing the sample through the chromatography column; and determining system suitability by comparing the amount of positive control detected to the negative control. In some embodiments, determining system suitability (e.g., sample inertness to the system) is accomplished by determining the ratio of positive control to negative control detected.

1. The name of the design product: the handle of chromatographic column. 2. The use of the design product: the whole is used as the chromatographic column of liquid chromatograph, and the part claimed is used as the handle of chromatographic column. 3. The design points of the design product: the shape claimed. 4. The picture or photo showing the design points most: the perspective view. 5. The part covered by pink color is not claimed.

The application discloses a separation and detection method of methoxy PEG derivatives and methylsulfonyl PEG derivatives, which comprises the following steps: mixing a sample to be detected, ammonia and an ammonium salt catalyst, carrying out a derivatization reaction to obtain a derivative product, and detecting the derivative product by using a liquid chromatography method. The sample to be detected is a methoxy PEG derivative or a methylsulfonyl PEG derivative sample. In the method, the stationary phase of the chromatographic column is octadecylsilane bonded silica gel, the mobile phase A is a 0.01-5% formic acid aqueous solution in terms of volume percentage concentration, the mobile phase B is acetonitrile and methanol in a volume ratio of (1-5):1, a gradient elution program is adopted, and an electrospray detector is used. The method can effectively separate and quantitatively analyze the methoxy PEG derivatives and the methylsulfonyl PEG derivatives, and the method is completely in line with the standards and performs well in the quantitative limit, the linear range, the accuracy and the like, thereby providing an effective guarantee for subsequent product quality control or evaluation.

This invention provides a method for preparing simian cyclophosphamide, comprising the following steps: loading simian cyclophosphamide extract into a macroporous adsorption resin column, performing first column chromatography using a first eluent to obtain simian cyclophosphamide component; loading the simian cyclophosphamide component into a reversed-phase silica gel column, performing second column chromatography using a second eluent to obtain crude simian cyclophosphamide; and loading the crude simian cyclophosphamide into a gel chromatography column, performing third column chromatography using a third eluent to obtain purified product. This preparation method utilizes a combination of macroporous adsorption resin column, reversed-phase silica gel column, and gel chromatography for purification, along with a specific elution procedure and eluent. The resulting simian cyclophosphamide I has a purity of over 99%, meeting the purity requirements for detection reference standards (>98%) and content determination reference standards (>99%), with an extraction yield of over 50%.

The application discloses a kind of bionic transmembrane protein affinity chromatography column and its preparation method and application.The system (iSTAC) realizes the in-situ construction of transmembrane protein in highly bionic dynamic microenvironment by integrating cell-free protein synthesis, functional mesoporous silica modification and amphiphilic (AH) peptide stabilized planar lipid bilayer technology.The core is to use long-chain PEG-24 crosslinking agent to retain lipid bilayer on the surface of silica gel, provide sufficient conformational dynamic space for multi-transmembrane protein, and introduce AH peptide to repair membrane defects, ensure that the receptor realizes directional embedding while maintaining natural functional attributes.The preparation cycle is shortened from 168 hours to 5 hours, which significantly improves the efficiency of targeted drug screening and in-situ analysis of binding sites.Using this platform, 5-HT 1A Receptor agonists crocin I and crocin II with anti-insomnia and neuroprotective effects are successfully screened from saffron, and the action site is accurately depicted.

The application discloses a method for simultaneously detecting 18 kinds of amino acids in Daqu based on high-resolution liquid chromatography-mass spectrometry, and belongs to the technical field of liquor detection. Chromatographic conditions of the method include that an ethylene bridge hybrid particle with surface-bonded amide groups is used as a filler, ammonium acetate-formic acid aqueous solution is used as mobile phase A, and acetonitrile is used as mobile phase B for gradient elution; mass spectrometry conditions include that an H-ESI ion source is used, the spray voltage in the positive ion mode is 3000-4000 V, and the spray voltage in the negative ion mode is 2000-3000 V; qualitative analysis is performed according to mass-to-charge ratios of the 18 kinds of amino acids, and quantitative analysis is performed by using a standard curve method. The method has the characteristics of simple pretreatment operation, easy-to-obtain reagents, high extraction efficiency and good stability, can realize synchronous and accurate qualitative and quantitative analysis of the 18 kinds of amino acids, and provides reliable data support for flavor substance research of Daqu.

The application relates to the technical field of silane gas detection, in particular to a silane gas purity rapid detection method, equipment and system, which comprises the following steps: collecting temperature data of an optical device surface in a gas chromatograph, pressure data inside a chromatographic column and vibration data at a support structure of a temperature sensor of the optical device surface in real time, detecting the purity of silane gas by using the gas chromatograph; obtaining environmental disturbance intensities at all collection moments; decomposing the environmental disturbance intensities at all collection moments to obtain a trend item, a periodic item and a residual item, and obtaining feature importance scores of the trend item, the periodic item and the residual item respectively, and then obtaining working condition degradation indexes when the purity of silane gas is detected at all collection moments; obtaining proportional coefficients of PID controllers at all collection moments, and controlling the pressure inside the chromatographic column when the purity of silane gas is detected. The application aims to improve the precision of silane gas purity detection by controlling the pressure inside the chromatographic column.

The application discloses a method for detecting 5-hydroxymethylfurfural in a compatibility experiment of hydrocortisone sodium succinate for injection and glucose injection by HPLC. The 5-hydroxymethylfurfural is a degradation impurity of glucose. The detection method comprises the following steps: (1) preparation of a control sample solution; (2) preparation of a test sample solution; (3) chromatographic conditions; a mobile phase A is 8mmol / L potassium dihydrogen phosphate solution (pH value is adjusted to 5.0-5.1 by using 8mmol / L potassium hydrogen phosphate solution, and the solution is prepared freshly before use); a mobile phase B is methanol, gradient elution is carried out; a detection wavelength is 284nm; a column temperature is 40 DEG C; a flow rate is 1.4-1.6mL / min; and a chromatographic column is C 18 ; the application can effectively detect the content of 5-hydroxymethylfurfural, and has strong specificity and high sensitivity.

Disclosed in the present invention are a single-column and double-column alternating cyclic chromatography system and method for separation and purification. The system comprises a first chromatographic column, a second chromatographic column, a first detector, a second detector, a first control valve, a second control valve, a first multi-way valve, a second multi-way valve, a third multi-way valve, a fourth multi-way valve, a fifth multi-way valve, and a sixth multi-way valve. The first control valve, the first multi-way valve, the first chromatographic column, the first detector, and the third multi-way valve are connected in sequence. The second control valve, the second multi-way valve, the second chromatographic column, the second detector, and the fourth multi-way valve are connected in sequence. The first multi-way valve is connected to the second control valve. The second multi-way valve is also connected to the first control valve. The second multi-way valve is connected to the third multi-way valve, the fifth multi-way valve, and the sixth multi-way valve. The fifth multi-way valve and the sixth multi-way valve are also connected to the first multi-way valve. The fourth multi-way valve is also connected to the first multi-way valve, the fifth multi-way valve, and the sixth multi-way valve. The present invention achieves a simple device structure, high separation efficiency, and a high yield.

This invention discloses a non-toxic liquid chromatography teaching aid, comprising a medium-pressure liquid chromatography column with an aspect ratio of not less than 10, activated amorphous silica gel, a syringe, a pre-dispensed mixture of target compounds, and pre-dispensed food-grade citric acid. The pre-dispensed mixture of target compounds is a mixture of carmine, tartrazine, and brilliant blue. This invention does not use harmful solvents or reagents, and the separation time is significantly reduced to less than 3 minutes. Furthermore, the chromatographic column used as a teaching aid can be reused multiple times. The actual separation cost is only pure water and food coloring solution, greatly improving the feasibility of chromatography teaching experiments.

This invention discloses a continuous or intermittent production equipment for obtaining high-purity natural products based on chromatography column purification. The equipment includes a chromatography column group, a multi-way valve group, and a control system. Through programmed control, each chromatography column in the production group sequentially undergoes the process stages of sample loading, water washing, low-concentration ethanol elution, and high-concentration ethanol desorption. After desorption is completed, the column is switched to the preparation group, and the regenerated chromatography column in the preparation group is seamlessly integrated into the production cycle, achieving continuous feeding and continuous output. The equipment system of this invention has a simple structure and clear control logic, which can significantly improve equipment utilization and production continuity, and is suitable for large-scale continuous production of natural products. Furthermore, the chromatography column group can also be used for intermittent production as a whole, simultaneously increasing the yield of high-purity natural products.

This invention belongs to the field of natural product chemistry, and relates to characteristic vibsane-type diterpenoids with a seven-membered ring skeleton from *Viburnum awabuki*, or their salts, as well as their preparation methods and applications. Specifically, this invention relates to 15 vibsane-type diterpenoids with a seven-membered ring skeleton extracted and isolated from the dried leaves of *Viburnum awabuki*, a plant in the Viburnaceae family. The vibsane-type diterpenoids were isolated by silica gel column chromatography, HP20 macroporous resin column chromatography, ODS column chromatography, and HPLC. The antitumor activity of these compounds was investigated by testing their inhibitory effects on three tumor cell lines: HepG2, A549, and MCF-7.

The application discloses a preparation method of polymyxin B sulfate impurity Z3 control product, and comprises the following steps: preparing a mobile phase, dissolving a sample containing polymyxin B sulfate, and then loading the sample into a chromatographic column, eluting the sample with the mobile phase, intercepting a fraction between a peak valley position of impurity B1-I and B1, and then re-loading and eluting the fraction to obtain a B1-I, Z3 and B1 triple peak after concentration, collecting a fraction corresponding to a peak part of Z3, repeatedly loading and eluting the fraction after concentration, and finally crystallizing to obtain a product Z3. The application has the advantages of simple operation, good separation effect and high-purity Z3 control product.

This application belongs to the field of seed detection and analysis technology, specifically relating to a method for constructing a characteristic chromatogram of Isatis indigotica seeds and a method for detecting Isatis indigotica seeds. The construction method includes the following steps: providing a reference solution; preparing a test solution using Isatis indigotica seeds; detecting the reference solution and the test solution using ultra-high performance liquid chromatography (UHPLC); importing the chromatograms obtained from detecting the reference solution and the test solution into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system to establish a characteristic chromatogram of Isatis indigotica seeds; the reference solution contains alkaloids as a reference standard; the UHPLC method meets the following conditions: the stationary phase includes a C18 column; the mobile phase includes mobile phase A and mobile phase B, mobile phase A including methanol and / or mobile phase B including water; and a gradient elution program is used. This application uses UHPLC to construct a characteristic chromatogram reflecting the secondary metabolites of Isatis indigotica seeds, providing a novel reference for evaluating the quality of Isatis indigotica seeds.

This invention belongs to the field of natural product chemistry technology, and relates to the extraction of natural products from the Saxifragaceae family (…). Saxifragaceae Juss.) *Changshan* ( Dichroa Lour.) Plant of the Chinese tallow tree ( Dichroa febrifuga Nine terpenoid compounds were isolated from *Dichroa febrifuga* extract. These compounds were obtained by sequentially separating the crude extract using D101 macroporous resin, silica gel column chromatography, HP20 column chromatography, ODS column chromatography, and HPLC. This invention also relates to investigating the bioactivity of these terpenoid compounds. The preparation method of this invention is simple, reproducible, and yields compounds with high purity, exhibiting good anti-inflammatory activity.

This invention relates to the field of succinic anhydride hydrolysis reaction detection technology, and discloses a method for detecting the succinic anhydride hydrolysis reaction rate using high performance liquid chromatography (HPLC). The method includes the following steps: (1) diluting the succinic anhydride hydrolysis reaction product with acetonitrile to obtain the test solution; (2) analyzing the test solution using HPLC, with a diode array detector, a reversed-phase column, and a mobile phase of a mixture of acetonitrile and water, and quantifying using the external standard method. The method provided by this invention utilizes ultra-high performance liquid chromatography (UHPLC) to simultaneously determine the content of succinic anhydride and succinic acid in the reaction solution, and has the advantages of rapid detection, simple method, and good repeatability.