The invention provides a preparation method of an active seaweed extract. The method comprises the steps of: seaweed selecting, impurity removing, weak acid washing, mincing and homogenizing, curing and sterilization under high temperature and high pressure as well as polysaccharide component degradation, pH adjustment, probiotic fermentation, and adding of alginate oligosaccharide for blending. The obtained liquid product is an active seaweed extracting solution, or it can be prepared into active seaweed extract powder through drying, and the extracting solution and the extract powder are collectively called an active seaweed extract. The active seaweed extract prepared in the invention reserves algae iodate, mannitol, inorganic salts, trace elements and other components, and the proteinand polysaccharide components in seaweeds are degraded and easy to absorb. Added with probiotics, secondary metabolites, and low molecular weight seaweed polysaccharide, the active seaweed extract ofthe invention has better biological activity, and can remove harmful heavy metals so as to improve the security of the seaweed extract.