809results about "Solid phase fermentation bioreactors" patented technology

It includes the stages of grinding the lignocellulosic biomass to a size of 15-30 mm, subjecting the product obtained to steam explosion pre-treatment at a temperature of 190-230° C. for between 1 and 10 minutes in a reactor (2), collecting the pre-treated material in a cyclone (3) and separating the liquid and solid fractions by filtration in a filter press (9), introducing the solid fraction in a fermentation deposit (10), adding a cellulase at a concentration of 15 UFP per gram of cellulose and 12.6 International Units of β-glucosidase enzyme dissolved in citrate buffer pH 4.8, inoculating the fermentation deposit (10) with a culture of the heat-tolerant bacteria Kluyveromyces marxianus CECT 10875, obtained by chemical mutagenesis from strain DER-26 of Kluyveromyces marxianus and shaking the mixture for 72 hours at 42° C.

According to one embodiment of the invention, a system for processing biomass comprises a chamber, a biomass input device, a fluid input device, and a retrieval device. The chamber is defined by at least a bottom, at least one wall, and a cover supported by the at least one wall. The biomass input device operable to deliver biomass into the chamber to form a biomass pile. The fluid input device is operable to deliver fluid into the chamber to the biomass pile. The retrieval device operable to receive fluid from the chamber.

This invention relates to a method and system of preparing ethanol based on the solid state fermentation of sweet sorghum stem, belonging to the ethanol production technology of solid state fermentation. It includes the following key processes and device: raw materials smash is to choose the suitable mill to smash sweet sorghum stalk, strain addition is to add ethanol bacteria at the entrance of solid-state fermentation tank, solid-state fermentation is that the raw materials added bacteria is fermented in the slant rotor solid-state fermentation tank, solid distillation is to distill the solid-state fermented materials to obtain ethanol.

By arranging reagents needed for a specific reaction in separate distinct chambers in a reagent cartridge and combining several such reagent cartridges in a cassette in a format that matches that of commonly used microtitre plates. e.g. the 96 well format or other standard formats. several drawbacks of well-known procedures can be eliminated.

The invention provides a microbiological solid-state seed producing and fermenting device, comprising a fermenting system, an air supply system, a temperature control system, a solid material system, a liquid-state material system and an electric control system, wherein the fermenting system comprises a fermenting tank provided with a stirring device, the top part of the fermenting tank is provided with a material liquid inlet, an exhaust port, a pressure gauge interface and an air inlet sequentially, a feed port is arranged on the upper right side of the front end of the fermenting tank, a discharge port is arranged on the lower left side of the front end of the fermenting tank, and the middle parts of both the feed port and the discharge port are provided with observation windows; the solid material system comprises a feeding device and a conveyer belt; the electric control system mainly comprises a stirring control unit, a temperature control unit, a material liquid control unit, an air supply control unit, a feeding and discharging control unit and a display. The device provided by the invention integrates the functions of steam sterilizing, fermenting, inoculating, drying and crushing of a solid culture medium, the automation degree is high, the microbial contamination rate is low, the production time is greatly shortened, the production efficiency is improved, and the stability of quality of a product is guaranteed.

The invention discloses a solid state fermentation cylinder and a solid state fermentation method. The solid state fermentation cylinder comprises a cylinder body and a jacket provided with an upper port and a lower port, the upper end of the cylinder body is an upper end cover, a mixing system capable of lifting is arranged on the upper end cover, and according to the mixing system, a mixing frame is driven by a driving system. The mixing frame is formed by a wall surface scraper plate, a bottom surface scraper plate and mixing blades, the wall surface scraper plate and the bottom surface scraper plate form a frame-shaped structure, and the mixing blades are fixedly connected in the frame-shaped structure. The solid state fermentation cylinder and the solid state fermentation method effectively resolve the problem of low fermentation performance caused by uneven thickness and uneven heating of a feed layer. According to the solid state fermentation cylinder and the solid state fermentation method, all the processes from feeding to discharging are concentrated in the solid state fermentation cylinder, the process of transportation of materials is simplified, and the mixing system and a cooling thermal-insulation system of equipment are strengthened. Automatic bulk production of solid state fermentation can be achieved, the pure culture in the solid state fermentation process is effectively guaranteed, quality and safety of products are improved, and homogeneity property in the same bulk is guaranteed.

The invention relates to a method for separating ethanol by using Chinese sorghum straw through solid fermentation method and the system, belonging to field of ethanol produ ction process through solid fermentation. It comprises following steps: disintegrating Chinese sorghum straw, solid fermenting and getting fermentation material, conveying it to distilling system through compact channel convey belt; feeding fermentation material into annular rotary disk, contacting with steam from high- efficiency gas distributor to make ethanol converse into gas phase from solid phase, discharging slag continuously; separating ethanol with distilling- rectifying coupled process, getting industrial ethanol of 95.5% (w/w). The ethanol productivity is increased by employing istilling- rectifying coupled process, the ethanol volatilisation is dramatically reduced during conveying process by employing compact channel blet convey; the transiation is improved and continuous production is realized by employing annular rotary disk for solid distillation.

The invention relates to an organic waste dry anaerobic high-temperature fermentation system and a fermentation process. The fermentation system comprises a feeding system (1), a dry anaerobic fermentation tank (2) with an agitating system (3), a heat preservation and heating system (4), a biogas slurry and biogas residue treatment system (5), an exhaust system (6) and a control system. The organic waste dry anaerobic high-temperature fermentation system and the fermentation process have the beneficial effects that harmless treatment, reduction treatment and resource treatment can be carried out on domestic organic wastes, so that the cyclic utilization of the wastes is realized; and moreover, the treatment efficiency is high, and the energy consumption and the treatment cost are low.

The invention discloses a full-automatic control multifunctional solid-state fermentation tank, comprising a round vertical fermentation tank main body and a fermentation control system, wherein a loading auger is arranged at one side of the upper end of the fermentation tank main body; multiple clapboards are arranged in the fermentation tank main body; a high-material-bed material level automatic control device is arranged in each fermentation layer; a sterile air distribution system is arranged in each fermentation layer and is connected with a sterile air generation tank positioned at the outer side of the fermentation tank main body; a main shaft is rotationally arranged in the fermentation tank main body; and the upper ends of the clapboards are provided with stirring blades. The full-automatic control multifunctional solid-state fermentation tank disclosed by the invention integrates eight functions including dry material mixing, watering material mixing, steam sterilization, inoculation, automatic fermentation control, low-temperature drying, raw material crushing, automatic discharging and finished product smashing, and enables all operations of solid-state fermentation to be completed in a multifunctional biological solid-state fermentation tank; and by virtue of the equipment disclosed by the invention, the fermentation and production preparation processes of solid biological products can be continuously completed.

The invention relates to a solid fermentation bioreactor. The reactor comprises a reaction casing , and a porous material layer separator plate (5) is arranged in the reaction casing to divide the reaction casing into an upper fermentation chamber (1) and a lower layer air chamber (3); the reactor also comprises a fermentation control system for controlling a stirring unit, a temperature control unit, a humidity control unit, an air feed control unit and a fermentation tail gas detection and processing unit, so as to realize real time monitoring and control on temperature, humidity, ventilation, stirring and tail gas. The invention realizes totally enclosing on-line fermentation and prevents extraneous pollutants from entering; the tail gas detection system carries out real time on-line monitoring on fermentation gas, and tail gas is treated by tail gas recovery equipment to protect the environment, thereby realizing coupling operation of temperature, humidity, ventilation, stirring and tail gas control system and ensuring necessary essential factors for microbe growth.

The invention discloses an integral fuel-ethanol production process based on saccharide raw materials, and a device thereof, which belongs to the technical field of biomass liquid fuel production. The technical process comprises the following steps: grinding saccharide crops and allowing the ground saccharide crops to enter a device integrating solid-state fermentation, ethanol gas extraction and pneumatic dregs drying; mixing the ground material with high-yield ethanol yeast liquid and completing fermentation in anaerobic environment under the condition of transmitting strengthening heat mass; separating the ethanol through gas extraction; and drying dregs rotated in a tube body of a fermentor through gas flow and discharging the dregs out of the fermentor. The invention has the advantages of simplifying the process flow of producing the ethanol through solid fermentation, realizing high-degree technical integration, transforming fermentable sugar in the ground material to the utmost extent, efficiently extracting the ethanol in solid fermentation materials, utilizing flue gas and superheated steam (CO2 or heated dry air) to separate the ethanol, utilizing the flue gas (or the heated dry air) to dry the dregs, saving material-conveying time, reducing energy consumption and embodying that a solid fermentation method for producing fuel ethanol has the advantages of high net energy ratio and low environmental load.

The invention discloses a spiral self-stirring dry fermentation device and a method. Fermentation cylinders are obliquely arranged at the bottom in a gas storage tank which is installed on a top board of a leachate holding tank; an enclosed inner cylinder is arranged at the central part in each fermentation cylinder along an axial direction; a leachate jet pipe is mounted in the inner cylinder; nozzles are distributed on the leachate jet pipe and extend out from the inner cylinder; spiral separator plates are distributed in each fermentation cylinder along an axial direction, and initiating terminals of the spiral separator plates are located behind a feed inlet; a lower end of each fermentation cylinder is provided with a material outlet, a slag removal opening communicating with the material outlet of the fermentation cylinder is mounted on a base plate of the gas storage tank, and a slag removal door is arranged on the slag removal opening; the leachate holding tank is provided with a leachate return pipe communicating with the gas storage tank and a pipeline communicating with a leachate inlet of the leachate jet pipe; electric heating pipelines are distributed in the leachate holding tank; and a biogas conveying pipeline communicating with the upper part of the gas storage tank is arranged at the upper part of the leachate holding tank. The invention also provides the method of using the device for fermentation.

A system for removing particulate, sulfur dioxide, nitrogenous oxides and other condensables from the flue gas of a coal, lignite, or other solid fuel combustor. The system can combine biomass filtration, anaerobic digestion, steam absorption refrigeration, and heat exchangers to minimize parasitic energy consumption, solid and liquid waste disposal and interference with combustion efficiency, while achieving near total reduction of contaminants without regard to sulfur or ash content of fuel. Gases at the exit of a multiclone of a fuel-combustor enter a waste heat boiler, generating low pressure steam that drives a steam absorption chiller. The flue gas which still contains small particulate material pass through a moving belt system conveying a mat of chopped biomass that can be impregnated with bacteria that feed on nitrogenous oxides. The mat is sufficiently thick and moist to entrap the remaining solids and smoke in the exhaust stream. The particulate-free flue gas passes through a staged heat exchanger which can utilize ambient air, pond water, and the refrigerated water from the steam absorption chiller to condense the water vapor in the exhaust stream. Sulfur oxides exits the flue gas stream with the condensed water vapor which can be processed in an anaerobic digester. Methanogenic bacteria within the digester can convert the mat and the acidic condensate into a biogas, a combination of methane and carbon dioxide, and plant growth media.

A bioreactor includes a nutrient reservoir, a rotatable culture bed, at least one culture medium, a mist generator and a mist delivery pipe. The nutrient reservoir stores therein a nutrient solution required for the fungus, plant cell, tissue, organ, hairy roots and plantlet. The rotatable culture bed is rotated at a specified speed. The at least one culture medium has thereon the fungus, plant cell, tissue, organ, hairy roots and plantlet and is supported on the rotatable culture bed. The mist generator is used for producing mists of the nutrient solution. The mist delivery pipe delivers therein the mists of the nutrient solution and has a mist-exit port adjusted to a desired level above the culture medium so as to discharge and spray the mists over the fungus, plant cell, tissue, organ, hairy roots and plantlet.

The invention discloses a dry type anaerobic fermentation tank, belonging to anaerobic fermentation equipment. Biogas slag and biogas slurry are added in a batching water-seal tank, a feed conveying assembly communicates the batching water-seal tank and a fermentation tank body, the fermentation tank body is internally provided with a feed-frame empty-mixing preventer driven by a first motor, a fermentation tank base is internally provided with a spiral discharging machine driven by a second motor, a screen, a wedge-shaped feed collecting groove and a heating pipe, an extrusion and discharge shell with a discharge hole is arranged on the side wall face of the fermentation tank base and is internally provided with a piston, a pressure spring and a spring seat, an end cover provided with a pressure regulating handle is arranged on the outer end face of the extrusion and discharge shell, the pressure regulating handle and the spring seat are connected together or in contact fit, one end part of the spiral discharging machine is located in the extrusion and discharge shell, a spraying connection pipe communicates a circulating pump and the fermentation tank body, and the circulating pump is communicated with a percolate tank. The tank has novel and reasonable structure, high production efficiency and wide applicable range, and is reliable to run and simple and convenient to use and operate.

The invention belongs to the field of microalgae culture, and particularly relates to an inserting-plate type microalgae semi-dry solid adherent culture device. The inserting-plate type microalgae semi-dry solid adherent culture device is characterized by comprising a supporting truss, culture plates, liquid distributing pipes, liquid collecting tanks and a liquid storage bottle, wherein the supporting truss is provided with at least one culture plate, and one liquid distributing pipe is arranged above each culture plate; the liquid collecting tank arranged on the supporting truss is arranged under each culture plate; all the liquid collecting tanks are communicated with the liquid storage bottle by the main liquid pipe; the bottom of the liquid storage bottle is respectively provided with a circulating pump and a carbon-supplementing gas distributing pipe communicated with a carbon dioxide source, and the circulating pump is communicated with a liquid distributing pipe by a pipeline; and each culture plate is provided with a picking scraper. The inserting-plate type microalgae semi-dry solid adherent culture device has the advantages that the yield in unit occupied area is effectively increased, the culture period is shortened, the water consumption for culture is greatly reduced, the cost and the operating cost of the culture device are reduced and the industrial culture on large scale is easily realized.