73 results about "Methanogenesis" patented technology

The application provides a fruit peel biochar and a preparation method and application thereof in reinforcing residual sludge methanogenesis, and belongs to the technical field of solid waste resource treatment. The preparation method of the fruit peel biochar comprises the following steps: drying, carbonizing, and grinding banana peels after being cut into pieces to obtain the fruit peel biochar; the heating speed in the carbonizing process is 10-15 DEG C / min, the pyrolysis temperature in the carbonizing process is 500-600 DEG C, and the holding time under the pyrolysis temperature is 1-2 h. The fruit peel biochar has the advantages of strong alkalinity, and the residual sludge is pretreated first, the hydrolysis rate of the residual sludge is improved, the barrier of macromolecular substance dissolution is broken, more organic substrates are provided for the subsequent anaerobic digestion process, meanwhile, the biochar added in the pretreatment stage can still reinforce the effect of the anaerobic digestion in the subsequent stage, and a more competitive new idea for the application of the biochar in the anaerobic digestion is provided.

The invention discloses a system and a method for segmented regulation and control of biomass anaerobic fermentation based on modified biochar. The system comprises a low-temperature biochar preparation system, a biochar modification system, a hydrolytic acidification system, a methane production system, a biogas purification and separation system and a heat utilization system. The low-temperature biochar preparation system is used for preparing low-temperature biochar and providing the low-temperature biochar to the hydrolytic acidification system and the biochar modification system, and the biochar modification system is used for carrying out carbon dioxide high-temperature modification on the low-temperature biochar to obtain modified biochar and providing the modified biochar to the methane production system; the biochar is modified according to different requirements on biochar functions in different stages of anaerobic fermentation, and low-temperature biochar and modified biochar are respectively added in a hydrolytic acidification stage and a methanogenesis stage of anaerobic fermentation, so that accurate intervention of C / N dynamic balance and inhibitor control is realized.

The invention belongs to the technical field of sludge treatment and recycling, and relates to a method for promoting anaerobic methane production by sludge through tubular pore structure biochar. Comprising the following steps: screening, washing, segmenting and drying a biomass material with a natural grade pore network, and then pyrolyzing and screening to obtain tubular pore structure biochar; carrying out sedimentation, sieving, dilution and pyrohydrolysis on the residual sludge to obtain pretreated sludge; mixing the pretreated sludge with the inoculated sludge to obtain mixed sludge; and adding the tubular pore structure biochar and the mixed sludge into an anaerobic reactor, aerating nitrogen in the anaerobic reactor, sealing the anaerobic reactor in a water bath constant-temperature oscillator, and carrying out methane production until no methane is generated in the anaerobic reactor. According to the method, the anaerobic methanogenesis efficiency of the residual sludge is remarkably improved by utilizing the tubular pore structure biochar, and the improvement of the anaerobic methanogenesis efficiency of the sludge under the mediation of the exogenous conductive material is realized.

The invention discloses a kitchen waste anaerobic digestion treatment and resource utilization system which comprises a pretreatment subsystem, an anaerobic digestion subsystem, a biogas purification and utilization subsystem and a biogas slurry and biogas residue resource subsystem which are sequentially connected along the material flow direction, the pretreatment subsystem is used for carrying out impurity separation, pulping and oil extraction on incoming kitchen waste; and the anaerobic digestion subsystem is used for carrying out hydrolytic acidification and methanogenesis reaction on the pretreated organic slurry to generate biogas. According to the invention, through integration of high-efficiency pyrohydrolysis pretreatment, two-phase anaerobic digestion enhancement and multi-element resource deep treatment, the spanning of kitchen waste treatment from reduction to high value is realized, and the beneficial technical effects of the system are reflected in that: through pyrohydrolysis and multi-stage separation pretreatment, the organic matter degradation efficiency and the grease recovery rate are remarkably improved; and the acidification risk of the system is effectively relieved by combining two-phase anaerobic digestion with a trace element strengthening strategy, and the biogas yield and the operation stability are greatly improved.

The utility model belongs to sewage treatment technical field, specifically is a kind of sludge reduction's sewage treatment equipment, including base, the base top is fixed with processing cylinder, the processing cylinder bottom is fixed with bottom plate, by setting movable cylinder, second movable shaft, stirring plate, stirring rod and stirring ring, can make sludge particle in sewage even suspended, let hydrolytic bacteria, methanogen etc. and the organic matter in sludge fully contact, accelerate hydrolysis, acidification and the rate of methanogenesis reaction, reduce substrate residue, and anaerobic sludge is kept for a long time, can form dense precipitate layer in processing cylinder bottom, lead to effective reaction volume shrinkage, stirring can make sludge keep suspended state, maintain the effective volume utilization rate of processing cylinder, while anaerobic treatment needs stable temperature, if not stirring, local temperature difference in processing cylinder is easy to appear, lead to microbial activity imbalance, by stirring can maintain the uniformity of sewage anaerobic environment, stable reaction condition.

The invention discloses a method for improving methane production by anaerobic digestion through hydrothermal electrochemistry, which comprises the following steps: dispersing livestock and poultry manure by using a water solvent to obtain a raw material solution, carrying out anaerobic digestion treatment on the raw material solution under external voltage through an electrochemical anaerobic digestion system, and dehydrating the material solution subjected to anaerobic digestion to obtain a biogas residue solution with adjusted solid content, carrying out a heating hydrolysis reaction under a low-oxygen or anaerobic condition; after the hydrolysis reaction, the feed liquid is directly introduced into a flash tank, so that the pressure of the feed liquid is suddenly reduced, the temperature is reduced along with the room temperature environment, macromolecular substances are cracked into micromolecular substances under the flash evaporation effect, and then centrifugal treatment is performed to obtain biogas residue pyrohydrolysate and livestock and poultry manure biogas residues. The method provided by the invention not only can produce methane at high yield, but also enhances the degradation effect of organic matters in the raw material solution.

This invention discloses an anaerobic bio-fermentation process for the targeted degradation of municipal sewage sludge, belonging to the field of sludge treatment technology. The process includes: pretreatment and targeted activation of components; staged hydrolysis and acidification for targeted production of short-chain fatty acids; methanogenic fermentation mediated by conductive materials coupled with a microbial electrolysis cell for assistance; multi-parameter dynamic control based on online monitoring and intelligent algorithms; magnetic separation, recovery, and recycling of magnetic conductive materials; in-situ biogas purification and product gradient reflux; solid-liquid separation and residue resource utilization. This invention achieves kinetic matching and metabolic enhancement of hydrolysis and acidification and methanogenesis through the deep linkage of four modules: targeted hydrolysis and acidification, enhanced direct interspecies electron transfer via a microbial electrolysis cell, magnetic material circulation, and intelligent control. Compared with existing technologies, the methane yield is increased by more than 40%, propionic acid accumulation is below 50 mg / L, the fermentation cycle is shortened to 8-10 days, and system energy consumption is reduced by 20%.

The invention discloses a method for rapidly constructing denitrification type anaerobic methane oxidation through hydrogen type methanogens, and relates to the technical field of sewage treatment. According to the method, anaerobic digestion sludge of a sewage treatment plant is taken for inoculation, and a methanogen-mediated denitrification type anaerobic methane oxidation process is constructed. Hydrogen-type methanogens in the anaerobic digestion system excite a reverse methanogenesis function under the condition that organic matters are deficient, and denitrification anaerobic methane oxidation is realized by coupling sulfate and nitrate with nitrogen and sulfur circulating microorganisms. Sulfate is used as a potential electron acceptor in sewage, and dissoluble methane is removed while sulfate is indirectly utilized through recessive biological sulfur cycle catalytic bridging anaerobic methane oxidation and denitrification. In addition, the anaerobic digestion sludge can be continuously inoculated with hydrogen-type methanogens, and compared with traditional denitrification type anaerobic methane oxidation archaea culture, the method is more convenient. Therefore, the denitrifying anaerobic methane oxidation rapidly constructed by the hydrogen-type methanogens is an effective measure for breaking through the deep denitrification of the sewage treatment plant and synchronously controlling and discharging the greenhouse gas.

The present technology generally relates to a feed supplement pre-mix comprising: between about 50% and about 90% dewatered Asparagopsis spp.; and between about 15% and about 50% edible oil, wherein the dewatered Asparagopsis spp. has a moisture content of between about 50% and about 80%.

A process includes identifying a hydrocarbon-bearing reservoir containing a hydrocarbon fluid and a brine having a sufficient salinity level to prevent or inhibit one or more of methanogenesis and sulphate-reduction activity, and responsive to a concentration of hydrogen being below 1000 parts per million in a sample collected from the hydrocarbon-bearing reservoir, introducing at least one non-native or indigenous hydrogen producing microorganism or stimulating the activity of at least one indigenous bacteria capable of stimulating a microbial consortium to increase a production rate of hydrogen in the hydrocarbon-bearing reservoir.

The present invention discloses the application of calcium peroxide pretreatment coupled with two-phase anaerobic digestion in promoting methane production. The invention utilizes calcium peroxide pretreatment technology to give play to the advantages of the separation of the acid-producing phase and the methane-producing phase in the two-phase anaerobic digestion process, effectively promotes the enrichment of methanogenic functional bacteria in sludge anaerobic digestion, greatly improves the methane production rate, shortens the methane production hysteresis period, and realizes efficient energy conversion of sludge.

The present invention relates to the field of degradation with hyperthermophilic organisms, and in particular to the use of hyperthermophilic degradation to produce heat and energy rich components including hydrogen and ethanol from a biomass. In some embodiments, a biomass is fermented in the presence of hyperthermophilic organisms to produce heat. The heat is used to heat a liquid which is used directly in a heat pump or radiant heat or to produce electricity or drive a steam turbine. In some embodiments, acetate is utilized as a substrate to produce energy by methanogenesis.

The invention discloses an efficient lignocellulose degradation compound strain and application thereof in producing methane by fermenting straws. The lignocellulose degradation compound strain is prepared from phanerochaete chrysosporium, oyster mushroom, phoenix mushroom and talaromyces pinophilus. When the composite strain is used for pretreating straws, the degradation rate of cellulose in the straws reaches 77.86%, the degradation rate of hemicellulose in the straws reaches 21.01%, and the degradation rate of lignin in the straws reaches 34.85%; when the pretreated straw and the excrement are subjected to anaerobic fermentation together, compared with a control group, the total gas production rate is increased by 86.28%, and the methane production rate is increased by 98.95%. Therefore, the invention provides a green and efficient straw resource utilization technology so as to synchronously realize the purposes of waste reduction, energy recovery and fermentation residue resource utilization.

The invention relates to a biological resourceful treatment method and system for decommissioned lithium battery brine discharge wastewater, and the method comprises the following steps: (1) dividing the pretreated decommissioned lithium battery brine discharge wastewater into a first part of wastewater and a second part of wastewater, inoculating the first part of wastewater with a pre-domesticated salt-tolerant functional flora, hydrolyzing and fermenting organic components to generate fermentation liquor containing short-chain carboxylic acid; and (2) mixing the fermentation liquor with a second part of wastewater, and carrying out carbon chain extension reaction to generate treatment liquor containing medium-chain carboxylic acid and long-chain alcohol products. According to the method, the special pollutant composition and the high-salt characteristic of the discharge wastewater are utilized, the methanogenesis process is inhibited without using expensive chemical agents or treating inoculums at a high temperature and the like, electron donors such as ethanol or lactic acid and the like do not need to be additionally added, the agent or energy consumption cost in the process is greatly reduced while the organic pollutants in the wastewater are subjected to high-value conversion, and the method is suitable for industrial production. The method has the remarkable advantages of simple operation, green process, low cost and the like.

The invention provides a method for regulating and controlling anaerobic fermentation grading through intermediate product feedback, in the method, the stage number of hydrolysis stages is divided according to the hydrolysis characteristic of a fermentation material, the stage number of acid production stages is divided according to the acid production characteristic of a hydrolysate, a methane production stage is set as a first-stage methane production reactor, and a second-stage methane production reactor is set as a second-stage methane production reactor; grading nodes of all stages of anaerobic fermentation are provided, efficient and stable operation of multi-stage anaerobic fermentation can be achieved, and the method is simple and easy to implement.

A method for enhancing anaerobic methane production and reducing sludge resistance genes in high-solid sludge using nitrogen-doped nanofiber-anchored nickel nanoparticles relates to a method for treating high-solid excess sludge. The method aims to address the technical issues of poor treatment efficiency and low methane production in existing anaerobic digestion systems using conductive materials. This method involves: 1. preparing a nickel nanoparticle precursor using a sol-gel method; 2. preparing nanofibers containing the nickel precursor by electrospinning; 3. preparing nitrogen-doped nanofiber-anchored nickel nanoparticles by high-temperature sintering; 4. adding high-solid sludge, inoculated anaerobic sludge, and nitrogen-doped nanofiber-anchored nickel nanoparticles to an anaerobic digestion reactor for anaerobic digestion. Compared to a blank control, the cumulative methane production and maximum production rate increased by 25.66% and 28.21%, respectively; the average COD degradation rate increased by 34.22%; and the resistance gene content decreased by 8.18% to 19.10%. This method can be used in the treatment of high-solid sludge.

The present invention provides a method for treating organic solid waste and wastewater and an anaerobic bioreactor system thereof, and relates to the field of anaerobic reactor fermentation technology. The anaerobic bioreactor system includes: a reaction tank body, a filter assembly, and a biogas circulation assembly; the reaction tank body includes an anaerobic hydrolysis and acidification reaction tank and an anaerobic methanogenesis reaction tank; the filter assembly can perform membrane separation treatment on the mud-water mixture and can discharge the filtrate from the anaerobic methanogenesis reaction tank; the biogas circulation assembly is connected to the anaerobic hydrolysis and acidification reaction tank and the anaerobic methanogenesis reaction tank for collecting biogas and returning the biogas. A reciprocating drive assembly drives the membrane filtration device to move back and forth, and removes the mud cake layer on the surface of the membrane fiber through shaking inertia. The present invention improves the efficiency of organic solid waste and wastewater treatment, improves the effluent water quality, and enhances energy recovery capacity through the comprehensive optimization of structural design, membrane separation treatment, and biogas recycling, showing significant environmental and economic benefits.

The present invention relates to the field of sludge treatment technology, and in particular to a process for promoting sludge hydrolysis and medium-chain fatty acid synthesis using ferrocene, comprising: S10, adding ferrocene to the sludge and adjusting the pH to 3.0-5.0; S20, adding hydrogen peroxide to carry out a Fenton-like reaction, wherein the ferrocene undergoes a solid-liquid phase transition during the reaction; S30, adding ethanol, anaerobic seed sludge, and culture medium to carry out anaerobic fermentation and carbon chain extension; ferrocene generates hydroxyl radicals in the Fenton-like reaction to destroy sludge flocs and cell wall membranes; the converted liquid ferrocene cation acts as an electron mediator in step S30 to improve electron transfer efficiency; the liquid ferrocene cation simultaneously inhibits ethanol oxidation and methanogenesis. The present invention combines the Fenton-like reaction with a biological anaerobic process to construct a sludge hydrolysis, fermentation, and carbon chain extension process based on ferrocene regulation, thereby simultaneously promoting sludge hydrolysis, enhancing medium-chain fatty acid synthesis, and inhibiting substrate competition reactions, thereby expanding the high-value recovery rate of sludge resources.

The disclosure relates to compositions and methods of bacterial species expressing endolysins for the prophylaxis and treatment of methanogen growth in the digestive tract of an animal. More specifically, the disclosure relates to agents, compositions, and methods for preventing and / or inhibiting growth of methanogens.

The present disclosure relates to a method of ensiling Asparagopsis sp., the method comprising the steps of (a) providing Asparagopsis sp. having a moisture content of between 60 and 94 wt%; (b) adding an ensiling agent comprising an organic acid having 1 to 7 carbon atoms or a salt of an organic acid having 1 to 7 carbon atoms; (c) mixing said Asparagopsis sp. with the ensiling agent thereby providing a mixture; and (d) storing the mixture from step (c) under dark and anaerobic conditions for a time period sufficient to reduce the pH of the mixture to 4 or less. The present disclosure also relates to ensiled Asparagopsis sp. comprising bromoform in an amount of between 0.3 to 7 wt%, based on dry weight, preferably between 1 to 6 wt%, more preferred 2 to 5 wt%. The present disclosure further relates to an anti-methanogenic feed supplement as well as a method of reducing methanogenesis in a ruminant, and the use of ensiled Asparagopsis sp.

Animal feed compositions for reducing methane emission and / or for inhibiting one or more methane producing organisms (i.e., methanogens). Also included are the use of said compositions to reduce methane emission from animals such as ruminant animals, to inhibit methanogenesis by one or more methanogens in said animals, and / or to increase milk yield and / or body weight gain of said animals is described, as well as related methods.

The invention discloses a method for improving the efficiency of producing methane by solid anaerobic fermentation of agricultural and forestry residues through wood fiber pretreatment, and belongs to the technical field of biomass energy utilization. The method comprises the following steps: carrying out one or more coupling modes of physical pretreatment, chemical pretreatment and biological pretreatment on crushed and screened agricultural and forestry residues; the pretreated agriculture and forestry residues are used as a fermentation substrate, the water content and the element proportion are adjusted, anaerobic digestion sludge is inoculated, solid anaerobic fermentation is conducted for 25-35 days at the temperature of 30-55 DEG C, and biogas is collected. According to the invention, the wood fiber structure and components are optimized through physical-chemical-biological single or coupled pretreatment, and the solid anaerobic fermentation biogas yield and methane purity can be effectively improved in combination with accurate regulation and control of the ratio of carbon, nitrogen, phosphorus and sulfur and adaptive fermentation conditions; the energy consumption, the reagent consumption and the pollution abatement cost in the biomass energy preparation process are obviously reduced.

The present application belongs to the technical field of organic waste anaerobic fermentation, and discloses a method for producing medium-chain fatty acids by using blue vitriol to promote residual sludge anaerobic fermentation, which uses residual activated sludge from a municipal sewage treatment plant as a fermentation substrate, inoculates anaerobic sludge, adds blue vitriol and a methanogenesis inhibitor, adjusts pH value, and carries out anaerobic fermentation to produce medium-chain fatty acids under the participation of an electron donor; due to the addition of blue vitriol, the electron transfer capacity of microorganisms in the carbon chain extension process can be strengthened, thereby improving the yield of medium-chain fatty acids in the fermentation product; the addition amount of blue vitriol in the system is 2-20 g / L. By introducing blue vitriol into the sludge medium-chain fatty acid production system and strictly controlling the addition amount of blue vitriol in the system, the present application uses blue vitriol as an electron mediator to strengthen the electron transfer efficiency of microbial fermentation in the sludge medium-chain fatty acid production process, thereby improving the yield of medium-chain fatty acids, and further realizing efficient utilization of sludge resources.

A process includes identifying a hydrocarbon-bearing reservoir containing a hydrocarbon fluid and a brine having a sufficient salinity level to prevent or inhibit one or more of methanogenesis and sulphate-reduction activity, and responsive to a concentration of hydrogen being below 1000 parts per million in a sample collected from the hydrocarbon-bearing reservoir, introducing at least one non-native or indigenous hydrogen producing microorganism or stimulating the activity of at least one indigenous bacteria capable of stimulating a microbial consortium to increase a production rate of hydrogen in the hydrocarbon-bearing reservoir.

The present application relates to wastewater resource treatment technical field, especially to a kind of magnetic field reinforced anaerobic reactor and wastewater resource treatment method.The magnetic field reinforced anaerobic reactor provided by the present application, including anaerobic reactor body and multiple magnetic blocks fixed on the outer surface of the anaerobic reactor body, wherein the multiple magnetic blocks are divided into multiple rows and circled on the outer surface of the anaerobic reactor body, and the magnetic blocks in each row are arranged in the order of left, upper, right and down according to the direction of magnetic field.The magnetic field reinforced anaerobic reactor of the present application can convert the movement of microorganisms in the reactor relying on water flow, bubble or itself rising or falling into cutting magnetic induction line movement by using magnetic field, convert kinetic energy into potential energy, enhance the electron transfer between acetogenic bacteria and methanogens, improve the utilization of organic matter in wastewater by acid-producing bacteria and the process of methanogens utilizing electron to reduce carbon dioxide to produce methane, and realize the improvement of energy utilization and methanogenesis efficiency in anaerobic reactor.

The application provides a device and method for regulating biological membrane niche to realize simultaneous methanogenesis and denitrification, and belongs to the technical field of water treatment, comprising a biological membrane reactor, a water distribution tank, a peristaltic pump, an aeration system and an electric stirrer, the biological membrane reactor is filled with biological membrane carriers, and an anoxic-anaerobic biological membrane layered structure can be formed inside the carriers. The application inoculates functional sludge, domesticates the biological membrane carriers, regulates aeration intensity to build a dissolved oxygen gradient, combines with microelectrode real-time monitoring of the dissolved oxygen and oxidation-reduction potential profile inside the carriers, realizes efficient cooperation of denitrifying bacteria and methanogenic bacteria in the same biological membrane reactor, integrates anaerobic digestion and denitrification process, has the advantages of low energy consumption, small land occupation and strong impact resistance, can simultaneously realize organic matter degradation and denitrification of high-concentration organic wastewater, optimizes carbon source utilization efficiency, reduces treatment cost, is suitable for treatment of various high-concentration organic wastewater, and has important theoretical innovation value and engineering application prospect.

The application discloses a biomass synthesis gas anaerobic digestion methanogenesis system, which comprises an inlet flow controller, a high-temperature reaction device, a medium-temperature reaction device and a fermentation liquid transmission device. The inlet flow controller is connected with the bottom of the high-temperature reaction device through a pipeline, so that the metered biomass synthesis gas can be transported to the high-temperature reaction device. The top of the high-temperature reaction device is connected with the bottom of the medium-temperature reaction device through a pipeline, so that the gas fermented at a temperature of 45-65 DEG C can be transported to the medium-temperature reaction device. The side of the high-temperature reaction device, the fermentation liquid transmission device and the side of the medium-temperature reaction device are sequentially connected through pipelines, so that the organic acid fermented can be transported to the medium-temperature reaction device. The medium-temperature reaction device is used for secondary fermentation of the gas transported by the high-temperature reaction device at 25-40 DEG C and decomposition of the organic acid. The system can ferment the biomass synthesis gas to obtain gas with high methane yield and raw material utilization rate. The application further discloses a biomass synthesis gas anaerobic digestion methanogenesis method.

The invention provides a preparation method of biochar capable of enhancing the efficiency of producing methane by anaerobic digestion of sludge. The biochar comprises the following components: straws and a 30% H2O2 solution. The preparation method comprises the following steps: drying straw particles, and performing pyrolysis to prepare primary biochar; washing the prepared primary biochar, drying, grinding, adding into a 30% H2O2 solution, stirring, and heating; and finally, centrifuging, cleaning and drying to obtain the final biochar. According to the method, the functional group structure on the surface of the primary biochar is changed by utilizing the oxidability of H2O2, so that the electron transfer capacity is improved, and when the primary biochar is added into a sludge anaerobic digestion treatment system, the accumulative methane yield can be increased by 42.4%-53.5%, and the maximum methane production rate is increased by 85.5%-98.2%.

This invention discloses a method for improving methanogenesis from lignocellulose based on a mixed-culture fermentation strategy, belonging to the field of anaerobic fermentation technology. The method includes the following steps: (1) inoculating activated *Trichoderma echinosporum* into a culture medium containing lignocellulose and loofah sponge as a biological carrier material for fermentation to obtain a fermentation broth; (2) activating activated sludge using a revitalizing solution; (3) inoculating the activated sludge from step (2) into the fermentation broth of *Trichoderma echinosporum* from step (1) for anaerobic fermentation to produce methanogens. This method achieves a methane yield of 262 mL / g VS, which is more than 30% higher than that of traditional anaerobic fermentation for methanogenesis, and is of great significance for improving the bioconversion of lignocellulose resources.

This invention relates to a method for enriching and expanding polylactic acid (PLA) anaerobic degrading microbial communities based on methanogenesis kinetics. The invention addresses the technical problem of current methods for enriching and expanding mixed functional PLA anaerobic degrading microbial communities, which rely on fixed culture times or passage cycles as transfer criteria and lack quantitative criteria directly related to the system's metabolic activity, resulting in low enrichment efficiency and unstable directional shaping effects. This invention uses PLA as the sole carbon source and, by monitoring the methanogenesis process and fitting cumulative methanogenesis curves, determines the time point corresponding to the maximum methanogenesis rate as the transfer timing, thereby achieving rapid, stable, and directional enrichment of PLA anaerobic degrading functional microbial communities. This invention can improve the enrichment efficiency and directional shaping effect of PLA degradation-related functional microbial communities, increase the relative abundance of plastic degradation-related functional genes, and can be used for anaerobic degradation and synergistic biological treatment of PLA waste.