The present application belongs to the field of
biotechnology engineering, and particularly relates to a
kombucha fermentation agent based on 3D printing and a preparation method and application thereof. Based on the 3D printing technology, a hydrogel type
kombucha fermentation agent is developed, which can be directly put into the
kombucha water for
fermentation, and through supplementing the dominant
yeast bacteria lacking in the later fermentation stage, continuous
inoculation for multiple times can be realized. The 3D printing design model is redesigned and improved, the kombucha fermentation agent containing
yeast bacteria is prepared, the service life of the kombucha fermentation agent for
continuous use is prolonged, and the good fermentation performance is maintained. The present application can form a
microbial consortium in the hydrogel inside the microbial
population of kombucha, can solve the problems of loss of bacterial film bacterial liquid, lack of standardized
inoculation amount and the like in the traditional kombucha
inoculation mode. The present application has the advantages of high fermentation efficiency,
reusability, freeze-
drying long-term preservation, easy storage and low cost, and has great application prospect for the commercialization of kombucha.