95 results about "Microbial consortium" patented technology

A method for preparing a microbial consortium composite remediation agent for synergistic treatment of PAEs and Cd. The preparation method comprises: preparing a functional microbial consortium; preparing a microbial consortium carrier; and preparing the microbial consortium composite remediation agent. In the involved microbial consortium composite remediation agent, the functional microbial consortium is subjected to cadmium-tolerance acclimation, thereby not only exhibiting broad-spectrum degradation activity against PAEs, but also overcoming the issue of reduced degradation efficiency of single functional strains under the stress of heavy-metal Cd pollution in composite contaminated soil. The involved composite remediation agent is modified with sulfhydryl groups, providing excellent passivation effects on heavy metals such as cadmium. In combination with the functional microbial consortium, the remediation agent can achieve the effect of removing PAEs and Cd from the soil synchronously.

The invention relates to a formulation of microbial consortia selected from (a) Bacilli group, (b) Pseudomonas group and (c) Actinomycete group having nitrogen fixing capacity, when the bacteria comes in contact with soil or any aqueous media it gets active, which further helps for plant growth and makes the soil environment more suitable for plant.

The invention relates to the technical field of microorganisms, in particular to the technical field of agricultural microorganisms, and more particularly relates to a cellulose degradation composite flora as well as a construction method and application thereof. The cellulose degradation composite flora comprises a cellulose degradation bacterium, namely humid cellulomonas CW35 and an auxiliary strain used for digesting an intermediate metabolite of the humid cellulomonas CW35, and the auxiliary strain is one or more of pseudomonas Taiwan CW19, bacillus amyloliquefaciens L3 or microbacterium algal M266-2. Therefore, the feedback inhibition effect of a product in the cellulose degradation process of the cellulomonas can be destroyed, the expression and enzyme activity of cellulose degradation related genes are remarkably improved, and the decomposition utilization of cellulose-degrading bacteria, namely the wet cellulomonas CW35, on cellulose-containing wastes is effectively improved.

The invention discloses a microbial flora screening device, and particularly relates to the field of microbial culture.The microbial flora screening device comprises an external placement disc, the top end of the external placement disc is fixedly connected with a corresponding clamping frame, the top end of the corresponding clamping frame is fixedly connected with two sets of fixing columns, the interior of the corresponding clamping frame is fixedly connected with a spring, and one end of the spring is fixedly connected with a clamping clamping block; when the piston positioning push block drives the inclined pushing spring to make contact with the bottom end of the interior of the hollow piston pipe, the inclined pushing spring is in a compressed state under the pressure of the inner wall of the hollow piston pipe, the medicament is converted into mist through the assembly mist spraying performance of the mist spraying connector, and the mist spraying connector located at the upper corresponding through-flow hole sprays upwards; the spraying position sprays towards a farther position due to spraying inertia, and the spraying connectors located at the lower corresponding through-flow holes spray downwards, so that the microbial colonies located under the spraying position provide nutritional agents, survival agents are comprehensively provided for the microbial colonies, and a good survival environment is provided for the microbial colonies.

The present disclosure provides microbial communities comprising an intact microbiome substitute capable of stable implantation in the gastrointestinal tract for the treatment of IBD, ulcerative colitis, and Crohn's disease, and methods of making and using the same.

The utility model relates to the technical field of flora screening, in particular to a microbial flora screening device which can disperse and push flora on the upper surfaces of a plurality of screening pieces, ensures the screening effect, and is convenient to clean and use next time. Comprising a main body, a discharging pipe, a plurality of supporting legs, a plurality of screening pieces, a cleaning assembly, a mounting assembly, a limiting assembly and a dispersing assembly, the discharging pipe is connected to the right side of the bottom of the main body, the supporting legs are mounted at the bottom of the main body to support equipment, the cleaning assembly is mounted at the bottom of the main body, and the screening pieces are mounted in the main body through the mounting assembly; the bottom of the mounting assembly is connected with the cleaning assembly, the mounting assembly is connected with the top of the body through the limiting assembly, and the dispersing assembly is mounted on the screening part.

The disclosure relates to genetically modified microorganisms of the genus Bacillus, for the improvement of phenotypes of plants, for example nitrogen availability for non-leguminous plants. Included are novel strains of the microorganisms, microbial consortia, and agricultural compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and agricultural compositions comprising the same, in methods for imparting beneficial properties to target plant species. In particular aspects, the disclosure provides methods of increasing desirable plant traits in agronomically important species, for example nitrogen fixation, utilization, regulation, uptake, acquisition, tolerance, and / or processing in plants.

A process includes identifying a hydrocarbon-bearing reservoir containing a hydrocarbon fluid and a brine having a sufficient salinity level to prevent or inhibit one or more of methanogenesis and sulphate-reduction activity, and responsive to a concentration of hydrogen being below 1000 parts per million in a sample collected from the hydrocarbon-bearing reservoir, introducing at least one non-native or indigenous hydrogen producing microorganism or stimulating the activity of at least one indigenous bacteria capable of stimulating a microbial consortium to increase a production rate of hydrogen in the hydrocarbon-bearing reservoir.

The disclosure relates to isolated microorganisms—including novel strains of the microorganisms, microbial consortia, and agricultural compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and agricultural compositions comprising the same, in methods for imparting beneficial properties to target plant species. In particular aspects, the disclosure provides methods of increasing desirable plant traits in agronomically important crop species.

Whole cell based biostimulant compositions and methods for improving agricultural productivity the compositions including: a microbial consortium having gammaproteobacterial methanotroph. As a result of these methanotrophs, the biostimulant composition enables plant performance improvement, utilization of methane, and facilitates improved nitrogen fixation in plants. The composition also helps in reducing the need of external chemical fertilizers for plant growth, development, performance and / or survival.

The present disclosure provides methods and systems for production of biostimulants that promote phosphate solubilization in plants. Embodiments described include methods of making a biostimulant composition in a bioreactor system that includes two or more containers arranged in series. The bioreactor system may include an established population of a phosphate-solubilizing microbial strain. The method may include operating the bioreactor system by transferring into the system an aqueous feedstock that comprises a microbial consortium, transferring working fluid between containers of the system, and collecting a product. The method may further include maintaining a concentration of a phosphate-solubilizing microbial strain in the system.

The present invention relates to the field of bio-microbial consortium technology, and specifically to a heavy oil bio-microbial consortium recycling system. The system includes a microbial consortium screening module, a reaction delay identification module, a structural fatigue assessment module, a proliferation behavior calculation module, and a microbial replacement recommendation module. The present invention improves the efficiency and economy of heavy oil biodegradation by screening microbial consortia adapted to different heavy oil environments and optimizing their recycling. By obtaining detailed status parameters of heavy oil samples and dividing the microbial culture environment, the metabolic capacity and environmental adaptability of the bacterial strains can be accurately assessed. The start-up delay time and product concentration peak of the microbial consortium degradation reaction can be monitored, and strains with high response efficiency can be identified. The microbial consortium structure is optimized and the response speed of the biodegradation process is increased, thereby enhancing the biological efficiency of heavy oil treatment. Furthermore, a detailed assessment of strain structural fatigue and proliferation behavior is performed, enabling dynamic optimization of microbial consortium iteration.

The invention provides a three-dimensional microbial flora biological hybrid system capable of efficiently generating electricity by utilizing lignocellulose hydrolysate, and belongs to the technical field of biological batteries. According to the invention, an artificial microbial flora SLB composed of S.oneidensis, Lactococcus lactis and Bacillus subtilis is preliminarily constructed, the bacteria in the artificial microbial flora SLB are respectively subjected to genetic engineering modification, then a carbon cloth anode is replaced by a carbon felt anode, a dispersion liquid GO is added, the electricity generation and conductivity of an anode electroactive biological membrane are further optimized, and the electrochemical performance of the anode electroactive biological membrane is further improved. And finally, a three-dimensional microbial flora biological hybrid system S7L1B1 (at) CFamp capable of directly utilizing the lignocellulose hydrolysate to generate power is constructed. And GO. According to the present invention, the MFC technology is introduced, such that the MFC technology has good lignocellulose hydrolysate degradation and biological power generation capacity, such that the new approach is developed for the high-value utilization of the lignocellulose resource, the solid theoretical basis and the practical guidance are provided, and the application development of the MFC technology in the lignocellulose resource conversion field is promoted.

The invention discloses a method for synthesizing acetic acid from carbon dioxide by using sludge microbial flora, which comprises the following steps: step 1, inoculation: taking sludge containing acetogenic bacteria as an inoculum, and adding the inoculum into a reactor containing a basic culture medium; step 2, establishing an initial environment: introducing mixed gas for providing a carbon source and an energy source into the reactor, aerating, and replacing air in the reactor; step 3, implementing a nutrition stress stage: after aeration in the step 2 is finished, sealing the reactor, and carrying out constant-temperature culture for 10-15 days; and 4, strengthening culture in a recovery period: after the step 3 is finished, connecting a mixed gas providing a carbon source and an energy source with the reactor, executing periodic constant-temperature culture, and regularly replacing the basic culture medium in the periodic constant-temperature culture process. The method is simple to operate, low in cost and high in acetic acid yield.

The invention discloses a tea tree pruning efficient degradation microbial combined bacterium, a microbial agent and application of the microbial agent, the tea tree pruning efficient degradation microbial combined bacterium comprises bacillus JKB11 and paenibacillus polymyxa JKB16, and the JKB11 and JKB16 combination in the tea tree pruning efficient degradation microbial combined bacterium can effectively deal with degradation of tea tree pruning; although the living enzymes of cellulose degrading enzyme (CMCase) and lignin degrading enzyme (lignin peroxidase (LiP) and manganese peroxidase (MnP)) are similar to those of JKB16, the three-element content and infrared spectrum indexes of the degradation product prove that the tea tree pruning degradation effect of the combination of JKB11and JKB16 is better.

Provided herein are methods and compositions for growing a plurality of different bacteria, where each of the plurality of bacteria is grown on a separate medium from the others, removing the bacteria from the media, and combining them to produce a microbial consortium.

The invention relates to a biofilter and a method for the oxidation of methane generated in industrial, agricultural and sanitary landfill processes, among others, where the methane oxidation is biological, requiring methanotrophic microbial consortia, and where copper mining tailings are added to stimulate methane oxidation mediated by said methanotrophic microorganisms.

The present disclosure relates to genetically modified microorganisms for improving the phenotype of a plant, such as the nitrogen effectiveness of a leguminous plant. The present disclosure includes novel strains of microbial bodies, microbial consortia, and agricultural compositions comprising the same. In addition, the present disclosure teaches methods of utilizing the microorganisms, microbial consortia, and agricultural compositions comprising the same in methods of conferring beneficial properties to target plant species. In particular aspects, the disclosure provides methods of enhancing desired plant traits in agronomically important species, such as fixation, utilization, regulation, absorption, acquisition, tolerance and / or processing of nitrogen in plants.

The present application relates to the field of microbial technology, and specifically provides a synthetic microbial consortium B2 for preventing and treating banana wilt and application thereof. The synthetic microbial consortium B2 is composed of three antagonistic bacteria S-1-56, S-2-42 and 1-119 of Pseudomonas genus isolated from the rhizosphere soil of banana. Foc The synthetic microbial consortium B2 has a significant synergistic inhibitory effect. The synthetic microbial consortium B2 can be applied to the rhizosphere soil of banana by irrigation and the like, effectively prevents and controls the occurrence and spread of banana wilt, has the advantages of environmental friendliness, stable prevention and treatment effect, strong adaptability and the like, and is suitable for the production practice of green and sustainable agriculture.

The present application relates to the technical field of microbial immobilization, and discloses a preparation process of a specific carrier immobilized multifunctional microbial flora composition, comprising: mixing multifunctional microbial flora with an inducer to produce extracellular polymers, to obtain pretreated bacterial solution; adding carboxymethyl deacetyl chitin to the pretreated bacterial solution and adjusting the pH value, to form a primary wrapping suspension; immersing a porous carrier in the primary wrapping suspension, spraying a sodium phytate and calcium lactate composite aqueous solution under a pressure difference environment, and constructing a concentration gradient crosslinking network on the surface and pores of the carrier through ion bridging, wherein the present application utilizes component protonation response mechanism and nonlinear crosslinking distribution to actively open diffusion channels under a specific acid-base environment, reduce metabolic product discharge resistance, and maintain long-term metabolic activity and structural stability of the microbial flora.

Methods for selecting a microbe for co-formulation with a carrier are provided. In some examples, the methods include identifying a microbe that comprises one or more dipicolinic acid (DPA) synthase genes, a microbe that expresses one or more DPA synthase proteins, and / or a microbe that produces DPA; and selecting the microbe for co-formulation with a carrier. The methods optionally include co-formulating the selected microbe with the carrier. In some examples, the methods include detecting one or more DPA synthase genes or one or more DpaA and / or DpaB proteins in a microbe. In other examples, the methods include detecting DPA in a microbe or medium containing a microbe, for example, utilizing a fluorescence assay. Microbial compositions including one or more microbes that comprise one or more DPA synthase genes, express one or more DPA synthase proteins and / or produce DPA are also provided.

A new microbial consortium for use in a rapid screening method for irritable bowel syndrome (IBS) and possibly as a target for the therapeutic treatment of an IBS condition in a subject.

The present disclosure provides methods and systems for production of biostimulants that promote nitrogen use efficiency in plants. Embodiments described include methods of making a biostimulant composition in a bioreactor system that includes two or more containers arranged in series. The bioreactor system may include an established population of a nitrogen use efficiency-promoting microbial strain. The method may include operating the bioreactor system by transferring into the system an aqueous feedstock that comprises a microbial consortium, transferring working fluid between containers of the system, and collecting a product. The method may further include maintaining a concentration of a nitrogen use efficiency-promoting microbial strain in the system.

A process includes identifying a hydrocarbon-bearing reservoir containing a hydrocarbon fluid and a brine having a sufficient salinity level to prevent or inhibit one or more of methanogenesis and sulphate-reduction activity, and responsive to a concentration of hydrogen being below 1000 parts per million in a sample collected from the hydrocarbon-bearing reservoir, introducing at least one non-native or indigenous hydrogen producing microorganism or stimulating the activity of at least one indigenous bacteria capable of stimulating a microbial consortium to increase a production rate of hydrogen in the hydrocarbon-bearing reservoir.

The present application belongs to the field of biotechnology engineering, and particularly relates to a kombucha fermentation agent based on 3D printing and a preparation method and application thereof. Based on the 3D printing technology, a hydrogel type kombucha fermentation agent is developed, which can be directly put into the kombucha water for fermentation, and through supplementing the dominant yeast bacteria lacking in the later fermentation stage, continuous inoculation for multiple times can be realized. The 3D printing design model is redesigned and improved, the kombucha fermentation agent containing yeast bacteria is prepared, the service life of the kombucha fermentation agent for continuous use is prolonged, and the good fermentation performance is maintained. The present application can form a microbial consortium in the hydrogel inside the microbial population of kombucha, can solve the problems of loss of bacterial film bacterial liquid, lack of standardized inoculation amount and the like in the traditional kombucha inoculation mode. The present application has the advantages of high fermentation efficiency, reusability, freeze-drying long-term preservation, easy storage and low cost, and has great application prospect for the commercialization of kombucha.

This disclosure provides microbial consortia to target Colletotrichum or Botrytis species that target flowers, such as those of blueberries, to control diseases caused by these phytopathogenic species. Methods of delivery of the consortia to plants, including pre-blossom and post-blossom stages.

The present invention relates to a method of producing a microbial consortium of bacteria capable of colonizing plant roots.

The present invention relates to the biotechnology industry, and specifically to the development of a microbial consortium that is useful in the fermentation of seaweed. This invention further describes a method for producing a new alternative protein that combines the mycelium from the microbial consortium and the components of the seaweed used in the fermentation. The fermentation process allows the release and concentration of bioactive compounds present in the seaweed, such as proteins, amino acids, phenolic compounds and beta-glucans, which contribute to the functional properties of the final product. Furthermore, the invention comprises a new alternative protein product obtained using this specific method, characterised by the integration of the bioactive compounds of the seaweed with the mycelium of the consortium, which results in a food product with improved nutritional properties, functional bioactivity and better digestibility compared to seaweed.