43 results about "Plant traits" patented technology

The invention discloses a molecular marker closely linked with peanut yield related traits and application, and belongs to the technical field of plant molecular genetics and molecular breeding. The molecular marker disclosed by the invention is Arahy0036; the nucleotide sequence of Arahy0036 in peanuts with low single plant yield is shown as SEQ ID NO: 1, and the nucleotide sequence of Arahy0036 in peanuts with high single plant yield is shown as SEQ ID NO: 2. The marker is used for detecting the gene locus related to the yield character of the peanut single plant, the single plant with high yield can be identified after seeds are harvested, other single plants are selected, reserved and eliminated, the breeding scale can be effectively controlled, and the progress and efficiency of variety selection breeding are improved.

The application provides application of a TT2-SCT1 / SCT2-WR2 regulation channel in plant heat stress tolerance. The application first discloses a new signal channel involved in plant heat tolerance: a TT2-SCT1 / SCT2-WR2 signal channel; wherein, TT2 is a negative regulation factor for regulating the heat tolerance of plants or protecting (maintaining) the yield of plants in Gramineae under high temperature, SCT1 / SCT2 are also negative regulation factors for regulating the heat tolerance of plants or protecting (maintaining) the yield of plants in Gramineae under high temperature, and WR2 is a positive regulation factor for playing a role, which is beneficial to maintaining the homeostasis in plants. The application has important theoretical significance for genetic improvement of plant traits.

The present invention improves the efficiency of substance introduction when a particle bombardment method is used to introduce, into a plant-derived tissue, at least one substance selected from nucleic acids and proteins for modifying a trait. A method for modifying a plant trait according to the present disclosure comprises: a step for washing a plant-derived tissue with water; a step for introducing, by using a particle bombardment method, particles retaining at least one substance selected from nucleic acids and proteins into the tissue washed with water; a step for obtaining plant bodies by growing the tissue into which the particles have been introduced; and a step for selecting a plant body of which the trait has been modified from the obtained plant bodies.

Described herein are methods and compositions for improving plant growth and other characteristics. The methods and compositions utilize bacteria and bacterial secretions incorporated into plant seeds to improve plant growth and other characteristics.

The present disclosure provides a method for evaluating a seedling plant character of a transgenic poplar and use of an overexpressed pagEXPA1 gene in promoting xylem development of the poplar, relating to the technical field of forest wood character determination. The method for evaluating the seedling plant character of the transgenic poplar in the present disclosure includes the following steps: S1. detecting whether or not a foreign gene is integrated into a poplar genome through PCR; S2. detecting the expression level of the foreign gene in the poplar; S3. conducting a microstructural analysis on stem vascular tissues of the transgenic poplar; and S4. separating fibers, ducts, and sieve-tube cells through fiber isolation, and staining the isolated fibers to observe the morphology and structure of the fibers, ducts, and sieve-tube cells.

The disclosure relates to isolated microorganisms—including novel strains of the microorganisms, microbial consortia, and agricultural compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and agricultural compositions comprising the same, in methods for imparting beneficial properties to target plant species. In particular aspects, the disclosure provides methods of increasing desirable plant traits in agronomically important crop species.

The present application relates to the technical field of ecological restoration, and discloses a shallow lake submerged plant community configuration method and device, the method comprising: establishing a submerged plant grading screening model; wherein the submerged plant grading screening model comprises habitat condition grading, substrate condition grading and water quality grading; screening a plurality of species of submerged plants using the submerged plant grading screening model to determine the species of the screened submerged plants; obtaining submerged plant trait parameters for the plurality of species, and calculating a comprehensive tolerance index for each species of submerged plant based on the submerged plant trait parameters for the plurality of species; determining a species priority based on the comprehensive tolerance index for each species of submerged plant; and configuring a shallow lake submerged plant community based on the species of the screened submerged plants, the species priority and engineering parameters. The present application comprehensively considers ecosystem resilience and species diversity, ensures functional complementary configuration of species, and guarantees long-term stability of submerged plant community configuration in water ecological restoration.

Circular nucleic acid constructs of less than 1000 nucleotides are disclosed for manipulating plant traits. At least a portion of the constructs are single stranded.

The application discloses a new gene TaGW1-5B for regulating wheat kernel weight and application thereof. The application provides application of TaGW1-5B protein or TaGW1-5B gene in regulating plant traits; the plant traits are plant kernel traits and / or plant root traits; the kernel traits are kernel weight and / or kernel length; the root traits are root length and / or root surface area. The regulation is negative regulation. The application also provides application of a substance for inhibiting TaGW1-5B gene in cultivating plants with changed kernel traits and / or changed root traits; the changed kernel traits are increased kernel weight and / or increased kernel length; the changed root traits are increased root length and / or increased root surface area. The application can be used for creating new wheat germplasm with higher unit yield, and has important theoretical significance and application value for improving kernel weight.

This invention relates to the field of soybean plant information analysis technology, and particularly to a method for analyzing the traits of the whole soybean plant based on three-dimensional point clouds. The method includes: employing a joint annotation method for soybean pod states based on three-dimensional point clouds to collect, annotate, and standardize the three-dimensional point clouds; constructing an improved SoftGroup multi-task learning model, where each module works collaboratively according to a preset logic, taking the three-dimensional point cloud of a single soybean plant as input, and outputting the target task results through point cloud feature extraction and instance clustering; performing multi-task joint training on the improved SoftGroup multi-task learning model, inputting the preprocessed three-dimensional point cloud of the soybean plant to be tested into the trained model, performing multi-task inference, refining geometric parameters through PCA, and counting the number of pods; removing the pod point cloud from the three-dimensional point cloud of a single soybean plant to obtain the main point cloud of the plant; extracting the plant skeleton from the main point cloud of the plant and obtaining the trait data of the plant skeleton.

Disclosed herein are methods of improving agronomic traits in crop plants through editing sequences within the 5′ untranslated region (UTR) of plant genomes.

The application provides application of RanGAP in regulation of symbiotic nodulation. A novel functional gene RanGAP (including RanGAP1 or RanGAP2) is disclosed, which encodes RanGAP protein with important biological function, and the protein is involved in symbiotic nitrogen fixation process of leguminous plants and rhizobium, and the application has significant meaning in genetic improvement of plant traits and soil improvement. An isolated promoter from RanGAP genome (upstream) is also disclosed, which has the function of driving gene-specific expression; more particularly, the promoter drives specific expression of the gene in root vascular bundle, lateral root / nodule primordium.

The invention belongs to the technical field of agricultural biology, and relates to application of a transcription factor CGA1 with an amino acid sequence of SEQ ID NO: 1 in improving the photosynthetic rate and biomass of plants. Expression of the transcription factor CGA1 in vascular bundle sheath cells of plant leaves can improve the photosynthetic rate and biomass of plants, and is beneficial to improvement of plant traits and cultivation of new varieties of plants.

The application provides a use of an AP1 gene, and relates to the technical field of agricultural molecular breeding. The AP1 gene or a coded protein thereof is used for regulating the agronomic traits of plants or preparing an inhibitor or a composition for regulating the agronomic traits of plants; the regulation of the agronomic traits of plants specifically comprises: increasing the photosynthetic rate of plant leaves under salt stress, increasing the chlorophyll content, promoting the germination rate and / or total leaf area per plant, and the like. The AP1 gene can be used as a target for regulating the traits of plants and applied to plant breeding. The AP1 gene can effectively regulate the fluctuating light use efficiency of plants, increase the photosynthetic efficiency and Rubisco protein content under high-salt stress, and increase the resistance to adversity such as high-salt conditions.

The present disclosure relates generally to a composition comprising nucleic acids adsorbed onto layered double hydroxide (LDH) nanoparticles coated in positively charged proteins, and methods of introducing the adsorbed nucleic acids into plants and plant tissues using the composition. For example, the LDH nanoparticles of the present disclosure can be used to deliver a nucleic acid of interest to a plant or part thereof for a range of purposes including, but not limited to, protecting plants from pests and diseases, plant trait manipulation, and / or plant gene editing. The present disclosure also generally relates to the use of lysozyme for enhancing uptake and internalization of LDH nanoparticles by plants and plant tissues. For example, the present disclosure relates to methods of enhancing uptake and internalization of a plant or plant tissue with LDH nanoparticles by pre-treating the plant or plant tissue with lysozyme prior to incubating the plant or plant tissue with the LDH nanoparticles. The present disclosure also relates to a method for preparing the composition.

This disclosure relates to genetically modified microorganisms for improving plant phenotypes, such as nitrogen use efficiency in non-leguminous plants. It includes novel strains of microorganisms, microbial aggregates, and agricultural compositions comprising them. Furthermore, this disclosure teaches methods for utilizing the described microorganisms, the microbial aggregates, and the agricultural compositions comprising them in methods for conferring beneficial properties to target plant species. In a particular aspect, this disclosure provides methods for enhancing desired plant traits of agronomically important species, such as nitrogen fixation, utilization, regulation, absorption, acquisition, tolerance, and / or treatment in plants.

The present application relates to agriculturally beneficial microorganisms, microbial compositions and consortia. The present disclosure relates to isolated microorganisms, novel strains including microorganisms, microbial consortia, and agricultural compositions comprising the same. In addition, the present disclosure teaches methods of utilizing the microorganisms, microbial consortia, and agricultural compositions comprising the same in methods of conferring beneficial properties to target plant species. In particular aspects, the disclosure provides methods of enhancing desired plant traits in agronomically important crop species.

The present disclosure relates to edited (genetically modified) microorganisms comprising consortia of microorganisms, and to agricultural compositions comprising said microorganisms, intended to increase the tolerance of plants to nematode pests and / or to directly reduce nematode populations in soil or root systems. In addition, the present disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and agricultural compositions comprising the same in methods of conferring beneficial properties to target plant species. In particular aspects, the present disclosure provides methods for improving desired plant traits and reducing undesired pests that affect crop performance in agronomically important crop species.

The disclosure relates to methods for disrupting the function of upstream open reading frames (uORFs) in the 5' untranslated regions (UTRs) of plant clock regulator genes by introducing or selecting mutations, for example, using a gene editing construct, such as CRISPR / Cas (including Cas9, Cas12, MAD7, or SHARC™), which contains a polynucleotide encoding a Cas enzyme and at least one guide RNA targeting a uORF sequence in a plant. These guide RNAs specifically target uORFs in 5'UTRs associated with main open reading frames (ORFs) encoding polypeptides that are homologous to, or share at least 30%–100% identity with sequences set forth specified SEQ ID Nos disclosed therein. The disclosure also covers polypeptides (uPEPs) encoded by uORFs within sequences set forth in additional specified SEQ ID NOs or their homologous nucleotide sequences.

The invention relates to a Ca < 2 + >-CAMTA-ERF109 transcription cascade module for regulating and controlling transcription of a plant drought-resistant gene. Specifically, the invention provides an application of a CAMTA-ERF109 signal channel in regulation and control of abiotic stress tolerance of plants. The invention explains a new signal channel participating in plant osmotic stress for the first time: a CAMTA-ERF109 signal channel; wherein the CAMTA and the ERF109 are both positive regulatory factors for regulating the osmotic stress tolerance of the plants. The invention has important theoretical significance for genetic improvement of plant traits.

This disclosure belongs to the field of plant genetic engineering and provides a method for cultivating high-yield crops. Specifically, it provides a method for cultivating yield-increasing genes and high-yield crops by mining and screening biological materials related to sugar transport proteins, and overexpressing the biological materials through homologous transgenesis and / or heterologous transgenesis to regulate plant traits. Compared with wild-type plants, the yield of transgenic plants is significantly improved.

The invention relates to the technical field of genetic marker screening of plant traits, in particular to a molecular marker method for screening dwarf wheat seeds and application thereof.The method comprises the steps that wheat seeds are cultured with a protective agent containing a trehalose-proline polymer and the like, and leaves are collected; extracting DNA by using an improved CTAB (Cetyltrimethyl Ammonium Bromide) method added with special components, and carrying out ultrasonic assistance; designing a fluorescent labeled primer containing a novel base analogue, adding a special additive during PCR amplification, and monitoring in real time; and detecting the amplification product by polyacrylamide gel electrophoresis and capillary electrophoresis, and judging whether the short strain related molecular marker exists or not. According to the method, the precision of screening the dwarf wheat seeds is high, multiple frontier technologies are fused, gene expression can be verified, and environmental adaptability is also considered; the method is simple and convenient to operate, low in cost and capable of shortening the breeding period, accelerating popularization of excellent varieties and assisting agricultural yield increase and sustainable development.

The invention discloses a plant genome nucleic acid fragment accurate insertion and plant in-vivo excellent nucleic acid screening method, and belongs to the technical field of biological gene engineering. The method comprises the following steps: (1) inserting an attb site into a plant genome, and carrying out genetic transformation on a plant to obtain a stably transformed plant; (2) constructing another transgenic vector containing a serine recombinase expression cassette, attp and donor donor fragments; and (3) delivering the transgenic vector into the plant cells in the step (1) through an agrobacterium tumefaciens-mediated plant genetic method, so that a fragment between the two attp sites on the T-DNA is accurately inserted into the plant genome. And carrying out character analysis and sequencing on the recombinant strain so as to obtain nucleic acid information associated with excellent characters of the plant. According to the method, the nucleic acid can be directionally inserted into a specific and consistent position, so that the result interference caused by the difference between the insertion position and the copy number is avoided, a researcher can accurately judge whether the plant character change is caused by the inserted nucleic acid or not, and the accuracy of a screening result is greatly improved.

The invention provides a chrysanthemum DgLsL gene and application thereof, and belongs to the field of plant genetic engineering. The sequence of the chrysanthemum DgLsL gene is as shown in SEQ ID No. 1, and the sequence of the encoding protein of the chrysanthemum DgLsL gene is as shown in SEQ ID No. 2. Chrysanthemum leaf discs are infected through an agrobacterium-mediated method, the gene is transferred into chrysanthemum, a DgLsL gene transgenic chrysanthemum strain is obtained, and the result shows that the number of lateral branches of the DgLsL gene transgenic chrysanthemum strain is increased. Therefore, the chrysanthemum DgLsL gene disclosed by the invention has the function of promoting the growth of the lateral branches of the chrysanthemum, and has a good application prospect when being applied to plant character improvement.

A plant leaf hyperspectral trait estimation method, device, equipment and storage medium are disclosed, and belong to the technical field of plant trait estimation. The method comprises the following steps: obtaining a plant leaf hyperspectral dataset; based on the plant leaf hyperspectral dataset, a trait estimation model is pre-trained in a self-supervised learning manner; in the process of pre-training the trait estimation model in the self-supervised learning manner, after data in the plant leaf hyperspectral dataset is embedded into a high-dimensional feature sequence, self-supervised learning is performed based on the high-dimensional feature sequence; the high-dimensional feature sequence comprises a plurality of high-dimensional features, and the high-dimensional feature is a feature in a hidden space; for a target trait estimation task, the pre-trained trait estimation model is fine-tuned; and the fine-tuned trait estimation model is used for estimating the target trait based on the hyperspectral data of the plant leaf. The method can accurately perform plant leaf hyperspectral trait estimation and can be widely applied to various trait estimation.

A genetically modified bacterium is described with a modification in one or more selected genes from: NAC, ptsH, iaaA, gltA, pga, sdiA, fimA1, fimA2, fimA3, fimA4, wzxE, bolA, iscR, fhuF, sodA, sodB, sodC, FNR, arcA, arcB, rpoS, sbnA, treA, treB, phoP, phoQ, yjjPB, ychM, dauA, actP, yusV1, yieL1, yieL2, yieL3, yieL4, pgaB, rafA, melA, uidA, manA, abfA, abnA, lacZ, and yusV2. Methods of using the genetically modified bacterium to provide fixed nitrogen to plants and compositions containing the bacterium are also provided.

This invention provides a method for improving cyclic electron transport activity, photosynthetic rate, and plant height in plants. The inventors focused on identifying targeted regulatory targets to enhance plant photosynthetic efficiency, and identified the jasmonic acid signaling pathway-related synthetic gene OPR3. Mutants of OPR3 exhibited increased cyclic photosynthetic electron transport activity, net photosynthetic rate, plant height, and leaf area. This invention provides a novel target for plant trait improvement.

The invention discloses an under-forest plant functional group construction method for a low-quality and low-efficiency man-made forest, which comprises the following steps: collecting massive under-forest shrub and grass layer plant samples and corresponding soil samples in advance, and measuring plant traits of each type of plant samples to form an original data set; associating plant traits with ecological system functions, and establishing a layered index system; generating a multi-level functional character database based on the original data set and the hierarchical index system; and analyzing the data in the database by adopting a judgment matrix and average random consistency index method to obtain a classification result of the under-forest plant functional groups. The multi-level character database of the understory plant multi-functional category established by the method can provide comprehensive data support for subsequent ecological restoration practice; and in combination with management suggestions proposed by land utilization type research, an optimization scheme is provided for transformation of low-quality and low-efficiency forests. And theoretical support is provided for ecological function optimization and technical implementation of low-quality and low-efficiency artificial forests.