29 results about "Biological Stress" patented technology

The application discloses a MaDREB1F gene, and a nucleotide sequence of the MaDREB1F gene is shown as SEQ ID NO:1. Experimental research results reveal a mechanism of MaDREB1F in mediating non-biological stress resistance of bananas, and determine new targets (MaAOC4, MaACO20 and MaERF11) of MaDREB1F, which are beneficial to design breeding strategies to improve the resistance of crops to cold and drought stress.

The application provides a corn new variety breeding cultivation device and cultivation method, relates to the corn variety breeding technical field, and includes a support platform, which is arranged with multiple groups of cultivation bins in an array; the cultivation bin is slidably connected to the support platform through a driving mechanism, and a HEPA filter screen is connected to the cultivation bin; both sides of the cultivation bin are provided with observation windows, and multiple observation mechanisms are arranged on the support platform; the front end of the cultivation bin is provided with a docking mechanism; the bottom of the front end and the bottom of the rear end of the cultivation bin are respectively provided with abutment mechanisms; the front end and the rear end of the cultivation bin are respectively provided with opening and closing mechanisms; the cultivation bin is provided with an environment simulation mechanism; the cultivation bin is connected with a stress application window; the application integrates multiple environment simulations, multiple stress applications, automatic observation and unit recombination, greatly improves the breeding efficiency, realizes the simulation of different regional soil-climate conditions, can accurately combine multiple biological and non-biological stresses, and speeds up the breeding process of the corn new variety.

The invention discloses application of a nifU2 gene in inhibition of plant cell ferroptosis, and belongs to the technical field of gene engineering. The invention provides an effect of a plant iron-sulfur cluster transporter nifU2 in regulation and control of plant iron-sulfur clusters, and solves the problem of the effect of the plant iron-sulfur cluster transporter in ferroptosis in the prior art. The nifU2 participates in the process of plant ferroptosis, and participates in plant antiviral response by inhibiting ferroptosis. According to the application, nifU2 is identified as a regulatory factor of ferroptosis in plant cells for the first time, and subsequent exploration of ferroptosis related host factors and regulatory mechanisms thereof under other biological stress conditions is facilitated.

This invention discloses an early detection and evaluation method and system for the abiotic stress tolerance of new forest tree germplasm. The method includes: collecting chlorophyll fluorescence indicators, phenotypic indicators, and physiological and biochemical indicators of forest trees under abiotic stress conditions, and performing data cleaning and standardization; performing principal component analysis on the multi-source standardized dataset to screen candidate indicators; performing grey relational analysis using the comprehensive score of forest tree stress tolerance as a reference sequence and the candidate indicators as a comparison sequence; determining the core indicators and their weights based on the variance contribution rate of the principal component analysis and the correlation degree of the grey relational analysis; obtaining the abiotic stress assessment index from the core indicators using a membership function; and classifying the tolerance level of forest tree germplasm using a clustering algorithm based on this index. This invention achieves early, rapid, and accurate evaluation of the abiotic stress tolerance of forest tree germplasm, and can effectively guide forest tree stress resistance breeding work.

The invention discloses an application of corn E3 ubiquitinase in enhancing the resistance of corn to corn stem rot and drought. The name of the corn E3 ubiquitinating enzyme is ZmWIPF2, and the corn E3 ubiquitinating enzyme is shown as a sequence 2 in a sequence table. When the corn is subjected to abiotic stress such as drought and biological stress such as stem rot, the ZmWIPF2 cooperates with related genes to regulate and control the corn to resist the stem rot and drought and respond to pathogenic bacterium infection and drought stress. The ZmWIPF2 gene contains a zinc finger structural domain and an RING-H2 structural domain, wherein RING-Ubox is an E3 ubiquitin ligase. Transgenic material verification proves that the gene can improve the stress resistance of corn, has important significance for breeding stress-resistant corn varieties, and is suitable for wide popularization.

The invention relates to the technical field of remote sensing detection, in particular to a device and system for estimating cultivated land yield, and the system comprises a spectral data acquisition module, an image data acquisition module, a soil and physiological data acquisition module, an environmental parameter acquisition module, a pest and nutrition deficiency distinguishing module and a yield estimation and correction module. The device comprises a central controller and a power supply assembly for providing detection endurance, wherein the central controller is in signal connection with a spectral data acquisition assembly, an image data acquisition assembly, a soil and physiological data acquisition assembly and an environmental data acquisition assembly. According to the method, diseases and insect pests and nutrition deficiency are accurately distinguished through multi-source data acquisition and cross validation, the yield loss is corrected by dynamically integrating biological stress and environmental factors, and the defects of similar phenotype misjudgment and low estimation precision in the conventional technology are overcome.

The application provides a method for improving biological stress resistance by using a dotP gene, which comprises the following steps: transferring the dotP gene into a host organism, and making the host organism express the dotP gene; the host organism is a plant or a microorganism; the dotP gene encodes a protein (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO. 2; (b) a protein derived from (a) by substituting, deleting or adding n amino acids in the amino acid sequence in (a) and having the activity of a quadruplet repeat sequence protein, wherein n is any integer between 1 and 4. By the technical scheme, the biological stress resistance of the plant or the microorganism is improved, and in particular, the dry resistance of the plant or the microorganism is improved.

This invention belongs to the field of biological control technology for agricultural diseases, specifically disclosing a method for controlling root rot of Panax notoginseng based on ricinoleic acid. Specifically, this invention provides the application of ricinoleic acid in inhibiting root rot pathogens, wherein the pathogens are... Fusarium oxysporum、Fusarium solani or Ilyonectria vredhoekensis The ricinoleic acid provided by this invention can not only effectively control root rot in Panax notoginseng, but also significantly improve the physiological state of plants under heavy metal and biological stress, and enhance the overall stress resistance and yield of Panax notoginseng. It has outstanding advantages and application potential as a multi-effect, environmentally friendly green plant protection product.

The invention relates to the technical field of animal protection, and discloses a wild animal early-warning protection method based on migration data analysis, and the method comprises the following steps: 1, collecting the motion data of a target animal; 2, judging a high-energy intrusion risk and sending a wake-up signal; 3, extracting an atmospheric visibility factor and a rainfall interference factor; step 4, adaptively modulating output parameters according to the atmospheric visibility factor and the rainfall interference factor; 5, early warning intervention: constructing a multi-dimensional biological stress index, and guiding the target animal to migrate; and step 6, calling data to carry out iterative correction on the braking capability coefficient. According to the method, the dynamic trigger radius containing the physical inertia impulse and the motion trend compensation is calculated in real time through the projection component and the acceleration module value of the ground velocity vector in the boundary normal direction, so that the warning range can be dynamically adjusted according to the running speed and the acceleration trend of the animal; and the interception success rate of the sudden intrusion behavior is improved.

The application belongs to the technical field of plant genetic engineering, and particularly relates to application of LOC_Os01g34560 gene and coded protein thereof in breeding high-temperature-resistant rice; the gene and the coded protein thereof are involved in high-temperature non-biological stress regulation in the flowering stage of rice, and affect the rice yield by affecting the seed setting rate; LOC_Os01g34560 is edited by using a conventional method, so as to change the expression level of the gene, and then new rice germplasm with different expression levels of LOC_Os01g34560 gene is obtained; the rice created by the application has no significant difference in the growth and development of plants and other agronomic characters compared with the parent control, but significantly affects the seed setting rate under high-temperature non-biological stress; the application provides a useful gene resource for genetic improvement of sterile lines, and has important breeding utilization value.

The application discloses a Pinus massoniana tandem zinc finger structure PmTZF1 gene and an expression protein and application thereof, and relates to the technical field of plant genetic engineering.The Pinus massoniana tandem zinc finger structure PmTZF1 gene is disclosed for the first time, a nucleotide sequence of which is shown as SEQ ID NO.1, and an amino acid sequence of which is shown as SEQ ID NO.2.The application constructs an expression vector pBI121-PmTZF1 of the Pinus massoniana tandem zinc finger structure PmTZF1 gene; the constructed expression vector of the Pinus massoniana tandem zinc finger structure PmTZF1 gene is transformed into Arabidopsis; and a transgenic Arabidopsis plant with improved non-biological stress resistance is obtained through cultivation and screening.Compared with a wild type plant, the root of the transgenic strain is significantly longer than that of the wild type, and the transgenic strain blooms and sprouts earlier; under drought stress, the transgenic strain normally blooms and bears fruits, has more branches, and has more silique quantity, length and other properties, which are significantly better than those of the wild type strain.

The utility model discloses a diabetic foot biological pressure feedback adjusting device which comprises a shoe body and a shoe pad arranged in the shoe body, the shoe pad is sequentially composed of a contact layer, a pressure reduction layer and a protection layer from top to bottom, the pressure reduction layer is divided into a plurality of independent pressure measurement areas, an air bag is correspondingly arranged in each pressure measurement area, and the air bag is connected with the contact layer. All the air bags are provided with deflation electromagnetic valves, one of the air bags is designated as a main air bag, the other air bags are auxiliary air bags, the main air bag is connected with the auxiliary air bags through an air conveying channel, the main air bag is provided with a first air conveying electromagnetic valve and is connected with an air pump, and the air conveying channel is provided with a second air conveying electromagnetic valve. A main control chip and an independent pressure sensing unit are arranged between the air bag in each pressure measurement area and the protective layer, and the pressure sensing units, the first air transmission electromagnetic valve and the second air transmission electromagnetic valve are all electrically connected with the main control chip.

The invention provides a preparation method of peony and ginseng peptide for enhancing immunity and tonifying qi and blood, and belongs to the field of nutritious food. The composition comprises a peony peptide extract and ginseng polypeptide, the peony peptide extract is prepared by the following steps: soaking peony seeds in an elicitor solution, and fermenting with rhizopus oligosporus; and separating and purifying to obtain the peony peptide extract. Fresh peony seeds are soaked in the elicitor, biological stress is simulated to activate the defensive reaction of the peony seeds, and protein accumulation is induced; the rhizopus oligosporus is fermented for biotransformation, protein degradation is promoted, and active substances are possibly generated. And carrying out subsequent extraction to obtain the peony peptide extract. The extract and ginseng polypeptide are mixed in proportion, bioactive components from different sources are integrated, small molecule peptide is easy to absorb, and the immune regulation function is enhanced through the multi-target synergistic effect. The whole process is based on plant physiological response and biotransformation, and the preparation efficiency and quality of the peptide are optimized, so that the final composition has the effects of enhancing immunity and tonifying qi and blood.

The invention provides a cultivation device and a cultivation method for new corn variety breeding, and relates to the technical field of corn variety breeding, the cultivation device comprises a supporting platform, a plurality of cultivation bins are arranged on the supporting platform in an array mode; the cultivation bin is connected to the supporting platform in a sliding mode through a driving mechanism, and an HEPA filter screen is connected to the cultivation bin in a penetrating mode; observation windows are arranged on the two sides of the cultivation bin, and a plurality of observation mechanisms are arranged on the supporting platform; a butt joint mechanism is arranged at the front end of the cultivation bin; bordering mechanisms are arranged at the bottoms of the front and rear ends of the cultivation bin; opening and closing mechanisms are respectively arranged at the front and rear ends of the cultivation bin; an environment simulation mechanism is arranged in the cultivation bin; the cultivation bin is in through connection with a stress applying window; according to the invention, multi-environment simulation, multi-stress application, automatic observation and unit recombination are integrated, so that the breeding efficiency is greatly improved; simulation of cultivation soil-climate conditions in different regions is realized, various biological and abiotic stresses can be accurately combined, and the breeding process of new corn varieties is accelerated.

The invention relates to the technical field of agricultural biology, and discloses a cultivation method for improving saline-alkaline tolerance of soybeans, which comprises the steps of saline-alkaline tolerance variety screening and seed pretreatment, micro-furrow structure construction, layered and positioned fertilization, sowing and moisture management, and dynamic regulation and control in a growth period. A physical-chemical synergistic salt barrier is formed in a root development space by constructing a wave type micro-furrow structure and cooperating with a layered positioning fertilization technology, the controllability of salinity concentration gradient in a soybean growth microenvironment is ensured, meanwhile, an ion barrier protective film is formed on seeds by using a composite stress-resistant seed soaking agent, and the salt concentration gradient of the seeds is improved. The osmotic stress of saline-alkali ions to germinated seeds can be blocked in real time, the biological stress resistance stability established between seedling emergence and root systems of the soybeans under the saline-alkali stress is guaranteed, and the physiological injury risk caused by saline-alkali stress is reduced; by integrating a drip irrigation system and a growth period dynamic dressing mechanism of a humic acid-based conditioner, the time matching accuracy of salt and alkali regulation measures and soybean physiological needs is ensured.

The invention relates to the technical field of plant breeding, and discloses a cultivation method of a southern rice black-streaked dwarf virus resistant rice variety, which comprises the following steps: using a multi-stress mimicry triggering agent to carry out early warning and composite stress treatment on rice seeds and seedlings; then, applying a broad-spectrum epigenetic remodeling stabilizer to perform epigenetic modification curing treatment on the stress response state of the plant to obtain an F0-generation plant; and carrying out multi-generation planting and directional screening on the F0-generation plants until genetic strains with stable characters are obtained. According to the method, a specific chemical preparation combination is applied in order in a specific time window on a parental plant, so that a heritable response memory aiming at biological and abiotic composite stress is established and subjected to epigenetic solidification. The final rice strain obtained by the method can stably inherit the southern rice black-streaked dwarf virus-resistant and drought-resistant compound character to the offspring, and the technical problem that the existing breeding method is difficult to efficiently cultivate the compound resistance variety is solved.

The application discloses a hybrid Liriodendron chinense LhTRX-h3 gene, an expression protein thereof and application, relates to the technical field of plant genetic engineering.The hybrid Liriodendron chinense LhTRX-h3 gene disclosed by the application has a nucleotide sequence as shown in SEQ ID NO.1 and an amino acid sequence as shown in SEQ ID NO.2.The application constructs an expression vector of the hybrid Liriodendron chinense LhTRX-h3 gene, transforms the expression vector of the hybrid Liriodendron chinense LhTRX-h3 gene into hybrid Liriodendron chinense, and cultivates, screens and obtains a transgenic hybrid Liriodendron chinense plant with improved non-biological stress resistance.The hybrid Liriodendron chinense LhTRX-h3 gene disclosed by the application can promote the plant to improve non-biological stress resistance, regulate plant growth and regulate the length-width ratio of plant leaves.

The invention discloses application of exogenous nitric oxide in relieving inhibition of strontium stress on tomato seedling growth, and belongs to the technical field of plant stress resistance. Under the strontium stress condition induced by 10 mmol / L SrCl2, key growth indexes such as plant height, root length, stem diameter, leaf area, fresh weight and dry weight of tomato seedlings can be remarkably improved by spraying 100 mmol / L SNP solution on leaf surfaces, oxidative damage and growth inhibition caused by strontium stress are effectively relieved, and the stress resistance of the seedlings is enhanced. According to the method disclosed by the invention, the condition that the exogenous NO relieves the strontium stress of the tomato seedlings and the concentration dependence is clear for the first time, a new technical approach is provided for high-quality cultivation of the tomatoes in the metal-polluted soil, and a theoretical support is also provided for research and development of a relieving technology for plants to deal with the abiotic stress of metal poison.

The invention relates to application of a microbial agent in improvement of stress resistance, plant protection and nutrition of crops. The invention provides a method for improving crop stress resistance and increasing yield. In the invention, the bacillus pumilus KN-601 is applied to crops in a foliage spraying manner, so that the growth of the crops can be promoted, the yield of the crops can be increased, and / or the biological stress resistance of the crops can be improved.

The invention relates to the technical field of gene engineering, in particular to application of a GhUGP1 gene in regulation and control of drought resistance of cotton. Phylogenetic analysis reveals that the GhUGP1 gene has high conservative property in the plant evolution process, and promoter cis-acting element analysis shows that the GhUGP1 gene contains rich stress response elements and indicates that the GhUGP1 gene participates in abiotic stress response. By constructing a GhUGP1 overexpression plant, it is found that the GhUGP1 overexpression plant shows a more drought-resistant phenotype, a lower in-vitro leaf water loss rate and a faster rehydration recovery capability under drought stress. Physiological index detection shows that active oxygen accumulation of overexpressed plants is remarkably reduced, antioxidant enzyme activity is improved, malondialdehyde content is reduced, callose deposition is reduced, and pore opening is reduced. Results show that the GhUGP1 plays a key role in maintaining moisture balance, enhancing active oxygen scavenging ability and regulating stomatal movement by positively regulating the drought resistance of the cotton.

The invention discloses a directional breeding method for anti-pollution algae species of haematococcus, which relates to the field of microbial breeding, and comprises the following steps: constructing a pollution factor library, and preparing a pollution inoculation solution based on the factor library; a multi-stress culture medium is prepared, and key components of the multi-stress culture medium are set as limited quantity simulation nutrition competition pressure; establishing an initial genetic diversity algae group through adaptive culture and artificial mutagenesis expansion; the method comprises the following steps: preparing a multi-channel micro-fluidic chip, wherein each channel of the micro-fluidic chip is divided into a plurality of independent micro-culture chambers; and injecting a suspension of haematococcus cells, a pollution inoculation liquid and a multi-stress culture medium into each micro-culture chamber. According to the method disclosed by the invention, a multi-mode biological pollution library of competitive hybrid algae, predatory protozoa and algae pathogenic bacteria is constructed, and the real composite stress environment of the open pond is simulated by combining the abiotic pressure of nutrition limitation and dynamic allelopathy stress, so that the anti-pollution survival rate of the bred algae strains in the actual open pond culture is greatly improved, and the breeding cost is reduced. The yield stability is obviously superior to that of a single stress breeding variety.

The invention discloses double-layer bionic coating probiotics constructed by in-situ self-assembly as well as a preparation method and application of the double-layer bionic coating probiotics. According to the preparation method disclosed by the invention, the chitosan layer and the lipid layer are sequentially constructed through electrostatic adsorption and hydrophobic self-assembly effects to form a double-layer bionic coating with a uniform structure and clear gradation, so that efficient protection on probiotics is realized. The preparation method completely adopts food-grade raw materials, is high in safety, simple in process, good in repeatability and suitable for continuous preparation; the obtained coating can keep the film layer structure complete under various physicochemical and biological stress conditions, the survival rate of thalli is remarkably improved, and meanwhile the physiological activity, the key enzyme function and the anti-inflammatory effect of the coating are maintained. Good balance is achieved among coating flexibility, structural stability and biocompatibility, gastrointestinal tract tolerance and delivery efficiency of probiotics are remarkably improved, and the coating is suitable for protection and application of probiotics in functional food, special medical food and pharmaceutical preparations.

The invention belongs to the technical field of molecular plant pathology, and particularly relates to a peanut bacterial wilt resistant gene AhPR10-54 and application thereof. According to the invention, through whole genome identification and bioinformatics analysis, 883 PR family genes (AhPR) are totally identified in peanuts, and the genes are distributed in 16 different subfamilies; further gene expression analysis shows that most AhPR genes can respond to induction of biological stress and abiotic stress; then 15 candidate genes are screened out for bacterial wilt inoculation treatment and plant hormone induction, and results show that AhPR10-54 and the like have significant up-regulation expression characteristics in disease-resistant peanut varieties. The AhPR10-54 gene is used for disease resistance identification, and the result shows that transient overexpression of AhPR-54 can induce hypersensitivity of plants and significantly improve the resistance of tobacco to ralstonia solanacearum. The invention provides an important basis for the cultivation of broad-spectrum disease-resistant varieties of plants.

The present application relates to natural system simulation technical field, specifically to a mountain park ecological restoration digital twin method and system, in the present application, by constructing multi-directional elevation profile sequence and calculating micro-landform fractal dimension, combining with ellipse fitting technology to generate roughness tensor matrix, accurately quantifying the structural difference of micro-landform in different directions, using water flow velocity vector projection in roughness tensor to analyze equivalent fractal dimension, realizing accurate calculation of surface runoff resistance coefficient dynamic response with flow direction, thereby significantly improving the solving accuracy of hydrodynamic equation under complex terrain, at the same time, introducing environmental biological stress intensity index, based on spatial Euclidean distance as distance attenuation factor and interspecific competition coefficient weighted summation, deeply coupling physical habitat hydrodynamic condition and biological community interspecific competition mechanism, using biological stress correction state transition probability, realizing dynamic prediction of vegetation community succession path.

The invention discloses a rice deubiquitination enzyme gene OsZUFSP as well as cloning and application of the rice deubiquitination enzyme gene OsZUFSP. The OsZUFSP is a deubiquitination enzyme gene separated from rice, is composed of 1245 basic groups, and encodes a ZUFSP type deubiquitination enzyme with the molecular weight of 46 kDa and positioned in a cell nucleus and cytoplasm, namely the OsZUFSP. The OsZUFSP participates in the rice ubiquitination removal process and responds to the biological stress response of southern rice black-streaked dwarf virus invasion. The phenotypes of mutant rice with the OsZUFSP gene knocked out and over-expressed transgenic rice are not obviously different from those of wild rice, but the resistance of the mutant rice with the OsZUFSP gene knocked out to the southern rice black-streaked dwarf virus is enhanced, the symptoms of diseased plants are relieved, the susceptibility of the transgenic rice with the OsZUFSP gene over-expressed to the southern rice black-streaked dwarf virus is enhanced, and the resistance of the mutant rice with the OsZUFSP gene knocked out to the southern rice black-streaked dwarf virus is reduced. The symptom of the diseased plant is aggravated, which indicates that the OsZUFSP gene positively regulates and controls the southern rice black-streaked dwarf virus to infect rice Therefore, the gene can be knocked out through a gene editing technology to improve the resistance of the rice to the southern rice black-streaked dwarf virus.

The utility model relates to a large-scale organism stress and culture device based on wireless communication of an underwater vehicle, which comprises a wireless communication subsystem, a control and power supply subsystem and a stress and culture subsystem, and communication between the underwater vehicle and the large-scale organism stress and culture device is realized by using a lora underwater antenna; logic control of stress and culture is realized by using the control and power supply subsystem; a multi-channel valve and a peristaltic pump are used for realizing fluid and culture of a solution; the hot stab male head and the hot stab female head are used for achieving communication of solution channels, and the solution replacement requirement is met. The deep-sea large-scale organism in-situ experimental device integrates wireless communication, organism acquisition, organism culture and stress, and meets the experimental requirements of environment stress and long-time sequence culture of large-scale organisms in a deep-sea ecosystem; and the large biological stress and culture device can realize underwater wireless communication, process change can be carried out according to requirements, meanwhile, solution replacement can be carried out, and a more flexible operation scheme can be realized.

The invention provides pseudomonas aeruginosa capable of solubilizing phosphorus and resisting abiotic stress and a growth promoting application, and belongs to the technical field of biocontrol inoculants. The invention provides a pseudomonas aeruginosa CCR1-6 resistant to abiotic stress, and the preservation number of the pseudomonas aeruginosa CCR1-6 is CGMCC (China General Microbiological Culture Collection Center) No.35747. The strain provided by the invention is screened from soil, has strong phosphate solubilizing ability, can antagonize various abiotic stresses, especially saline-alkaline tolerance, acid resistance and drought resistance, and is short in growth cycle. The strain CCR1-6 can be prepared into a biocontrol inoculant, can promote the growth of crops in a single stress environment, can promote the growth of plants in a complex soil environment, can improve the stress tolerance of the plants, is high in phosphate solubilizing capacity, is suitable for activation of phosphorus in cultivated land soil, and can be used for preparing the biocontrol inoculant. By utilizing the strain, the content of available phosphorus in cultivated land soil can be remarkably increased, the application of phosphate fertilizer is reduced, and the application value is huge.

The invention discloses a plant physiological status monitoring method and application thereof, and the monitoring method comprises the following steps: building an array contact unit on the surface of a plant tissue to be detected, and building electrical contact for the array contact unit; applying electrical stimulation of different frequencies to the to-be-tested plant tissue of the contact unit which establishes the electrical contact so as to establish a simulation equivalent circuit, and collecting response current signals generated corresponding to different frequencies; calculating an impedance value under each frequency according to the collected response current signal, obtaining an electrochemical impedance spectrum, and establishing a multi-dimensional data set of the corresponding frequency; extracting characteristic parameters from the multi-dimensional data set to obtain electrochemical characterization signals; the electrochemical characterization signal is compared with a standard signal model, the physiological state change of the plant tissue to be detected is recognized according to a result, an electrochemical impedance spectrum of the plant is obtained by establishing array type electrical contact and applying multi-frequency electrical stimulation, physiological state abnormity is recognized earlier, and the method is used for early monitoring of plant biological or abiotic stress response.