58 results about "Biological Stress" patented technology

A method and system of differentially manipulating cells where the cells, suspended in a fluid, are irradiated with substantially monochromatic light. A Raman data set is obtained from the irradiated cells and where the data set is characteristic of a disease status. The data set is assessed to identify diseased cells. A Raman chemical image of the irradiated cells is also obtained. Based on the assessment and the Raman chemical image, the fluid in which the cells are suspended is differentially manipulated. The diseased cells are directed to a first location and other non-diseased cells are directed to a second location as part of the differential manipulation. The diseased cells may be treated with a physical stress, a chemical stress, and a biological stress and then returned to a patient from whom the diseased cells were obtained prior to the irradiation.

Methods of preventing and treating gastrointestinal dysfunction, particularly postoperative ileus and post-partum ileus, in a patient undergoing surgery or other biological stress by administering 4-aryl-piperidine derivatives are disclosed.

The present invention discloses a non-social pollution drug fertilizer for preventing wireworm and underground pest and the relative technics process; the drug fertilizer consists of the following components according to the weight ratio: 1-10 percent of bitter bean, 1-10 percent of cattle heart Preserving Simplicity, 1-10 percent of azedarach fruit, 1-3 percent of carapace or chitosan, 0.5-2 percent of salicylic acid or natrium salicylicum, 1-3 percent of Chlopyrifos or phoxim, 1-5 percent of diffusing agent, 1-5 percent of surface active agent, 1-10 percent of adhesive agent, 5-15 percent of disintegrant, 5-20 percent of micro fertilizer, 10-50 percent of corrupted acid; wherein, the weight ratio of all components is 100 percent. The drug fertilizer in the present invention integrates nutrient, improving soil and preventing pest together, which has much better immunity, prevention and curing effect to the wireworm and underground pest. The present invention can realize the purpose for ''detoxification to the soil and epidemic prevention to the crops'', decrease the use quantity for fertilizer and venomous pesticide and enhance the biological stress for the soil and crops. The present invention does not leave in the crop and pollute the environment. The present invention can accelerate the growth for the crops, decrease the pests in the crops, increase the output and improve the quality.

The invention discloses an ecological BB fertilizer, relating to a mutifunctional ecological environment friendly compound fertilizer, which is formed by mixing functional urea, controllable slow release sulfurcoated urea, monoammonium phosphate, diammonium phosphate, potassium sulfate particle (or potassium chloride), agricultural magnesium sulfate particle, ammonium sulfate, bio-organic inorganic compound fertilizer, endophytic PASP fertilizer synergist DA-6, plant growth regulator and trace elements. Special ecological BB fertilizer for different plants can be prepared by soil testing formula, according to different farm crops and different soil structure. The ecological BB fertilizer can take effect for 3-120 days, the nutrition release is balanced, biological stress of the farm crop such as drought resistance, cold resistance, salt resistance, or the like can be improved. The product of the invention has great operation significance for increasing yield of grain, reducing production cost, steadily increasing grain production, keeping rural incomes growing, improving utilization rate of the fertilizer, reducing fertilizer waste, protecting ecological environment, guaranteeing farm crop quality and safety, and ensuring sustainable development of the agriculture.

A wearable biometric device according to various exemplary embodiments can be configured to define one or more geo-fences relative to one or more physical locations; detect a geo-fence crossing when the wearable biometric device crosses any of the geo-fences; activate a geo-fence application and a biometric application, in response to the detection of a geofence crossing; monitor financial transactions of the user while the wearable biometric device is located within the geo-fence; activate a biometric sensor to detect biometric conditions of the user and determine if the biometric conditions of the user exceeds a predetermined threshold; activate a stimuli generating unit to detect if the user experiences a biometric stressor while the wearable biometric device is located within the geo-fence and activate the stimuli generating unit to apply one or more stimuli to the user until the biometric stressor experienced by the user is below the predetermined threshold.

The invention discloses a processing method and equipment of organic rubbish and wastewater, which is characterized by the following: utilizing the resource in the organic rubbish and wastewater to the maximum limit; adopting the enriched and cultured inoculation agent in the self-biological pressure anaerobic ferment course to transmit into stable strengthened humus organic fertilizer.

The invention relates to the technical field of crop disease control, in particular to a plant disease resistance gene NtWRKY50, and application of the plant disease resistance gene NtWRKY50 in bacterial wilt resistance of tobacco. A nucleotide sequence of the plant disease resistance gene NtWRKY50 is shown as SEQ ID NO. (sequence identifier number) 1. The gene NtWRKY50 is further transferred intothe tobacco for functional verification; a result shows that NtWRKY50 is a nuclear localization protein; expression of the gene is induced by pathogenic bacterium infection of plant bacterial wilt; overexpression of NtWRKY50 can improve the resistance of the tobacco to the bacterial wilt; silence of the gene NtWRKY50 has no significant influence on the plant disease resistance; NtWRKY50 can be induced by various biological and non-biological stresses; and the overexpression of NtWRKY50 significantly raises an SA level but inhibits JA accumulation induced by a pathogen. Data show that the overexpression of NtWRKY50 results in changes of SA and JA contents; the expression of defense related genes is increased; and the ralstonia solanacearum resistance of a plant is improved. A gene resourceis provided for cultivating a novel bacterial wilt resistance tobacco variety by a gene engineering manner, and the gene has an important application prospect.

A continuous changeable stress test system relates to a biological test device and also comprises a stress test device and a signal receiving control device. The stress test device comprises a stepping motor, a push-rod transmission device, a pressure spring device, a pressure sensor and a push plate. The power output end of the stepping motor is connected with the power input end of the push-rod transmission device, the pressure spring device is arranged at the top of the mandril of the push-rod transmission device, the pressure sensor is arranged at the top of the pressure spring device, the push plate is arranged at the top of the pressure spring device, the instruction output end of the signal receiving control device is connected with the instruction input end of the stepping motor, and the data transmission end of the pressure sensor is connected with the data receiving end of the signal receiving control device. The continuous changeable stress test system can generate continuous-changing stress and acquire biological stress and biological electro-myographic signals synchronously; and through a contrastive analysis of the relationship between biological stress generation and the biological surface electro-myographic signals, can be used for muscular atrophy degree detection.

The invention provides a method for simulating a biological stress reaction to induce gingkoes to quickly accumulate bilobalide, and belongs to the technical field of biological techniques and botanytechniques. Gingko plants are cultured through simulation of natural growth conditions, jasmonic acid methyl ester (MeJA), namely a plant hormone, is adopted for inducing, and a high-performance liquid chromatography-evaporation light scattering method is adopted for detecting and analyzing the dynamic change condition of accumulation of the bilobalide. The change conditions of the bilobalide content are detected under different inducing concentration and time conditions, and therefore the concentration and inducing time of MeJA are determined, so that the optimum inducing condition of the MeJA for inducing fast accumulation of the bilobalide is screened out, really effective bases are provided for increasing the yield of the bilobalide, and a scientific basis is provided for treatment before harvesting of ginkgo leaves.

The invention discloses rice dehydrin protein with antibacterial activity. The dehydrin protein contains one of the following amino acid residue sequences: (1) protein of SEQ ID NO:1 in a sequence table; (2) protein with antibacterial activity, which is obtained by replacing and/or deleting and/or adding amino acid residues of the SEQ ID NO:1 in the sequence table by one or several amino acid residues; and (3) segments with over 15 amino acids of the SEQ ID NO:1 in the sequence table. The in-vitro expressed rice dehydrin protein, the protein segments and in-vitro synthesized segments in the invention all have antibacterial activity for special Gram-positive bacteria. The dehydrin protein in plants, disclosed by the invention, is extracted for inhibiting the Gram-positive bacteria. Resistance to biological stress can be improved by transplanting encoding genes of the rice dehydrin protein provided by the invention into plants.

The invention belongs to the field of gene cloning in molecular biology, and particularly relates to a hyperaccumulating black-nightshade metallothionein like (MT-L2) gene sequence and a cloning method thereof. The hyperaccumulating black-nightshade metallothionein like (MT-L2) gene sequence is a 2-type metallothionein gene with a gene sequence shown as the nucleotide sequence in the SEQ ID NO.1 or SEQ ID NO.2. Success cloning and sequencing of the hyperaccumulating black-nightshade metallothionein like (MT-L2) gene sequence lay foundations for further analysis of biological stress resistance under the influence of MT-L2, and for screening and applications of other functions of the MT-2.

The invention discloses a litopenaeus vannamei sodium-calcium exchanger LvNCX gene and a coding protein thereof. A nucleotide sequence of the litopenaeus vannamei sodium-calcium exchanger LvNCX gene is as shown in SEQ ID NO.1; and an amino acid sequence of the coded sodium-calcium exchanger LvNCX protein is as shown in SEQ ID NO.2. According to the invention, sodium-calcium exchanger LvNCX gene is firstly cloned out from the litopenaeus vannamei, and the gene is proved to be capable of sustaining equilibrium of sodium and calcium ions in a cell body by virtue of experiment, so that theoretical basis is laid for further deeply analyzing biological stress-resistance ability under NCX (Sodium-Calcium Exchanger) influences and applying the NCX gene in stress-resistance research of the litopenaeus vannamei.

The invention provides a high-expression-activity rice non-seed expression promoter SAFES4 and application thereof. The invention also provides an expression box and a crop expression vector, wherein the expression box contains the promoter. Concretely, the promoter SAFES4 provided by the invention can be used for regulation and control by aiming at the defect that foreign genes can realize the concentrated expression in the plants but cannot express in embryon and endosperm. The promoter is used for performing gene modification on the crop varieties; the integral growth features and the mechanism of the rice can be improved; the partial foreign gene expression of the eating part cannot be caused, so that the practical and effective transgene plant varieties can be cultured; the influence on the human eating part can be reduced; the misdoubt of people on the transgene food can be reduced or even eliminated. The promoter provided by the invention can specially drive the foreign genes to be expressed in roots/flowers/green tissues/non-seed tissues; the defect that the green tissue specific expression promoter cannot generally meet the root expression requirements is effectively overcome; great competitive power is realized in the biological stress resistance property improvement.

A wearable biometric device to mitigate cognitive dissonance according to various embodiments can be configured to define one or more geo-fences relative to one or more physical locations; detect a geo-fence crossing when the wearable biometric device crosses any of the geo-fences; activate a geo-fence application and a biometric application, in response to the detection of a geofence crossing; monitor behavior transactions of the user while the wearable biometric device is located within the geo-fence; activate a biometric sensor to detect biometric conditions of the user and determine if the biometric conditions of the user exceeds a predetermined threshold; activate a stimuli generating unit to detect if the user experiences a biometric stressor while the wearable biometric device is located within the geo-fence and activate the stimuli generating unit to apply one or more stimuli to the user until the biometric stressor experienced by the user is below the threshold.

The invention discloses a biological sound wave water quality safety monitoring method. Organisms will generate stress response in a polluted water body, and thus strong sound wave signals can be generated. Due to different pollution levels and different time exposing to pollutants, organisms will have different stress states, and the sound wave signals are different under different stress states. During the application process, a digital camera technology is used to analyze the sound wave signal energy so as to determine the different stress state of organisms in a water sample and corresponding characteristic peaks in a sound wave signal energy diagram. Then according to the sound wave signal characteristic peaks, the stress states of organisms can be determined directly. The sound wave monitoring system is simple and convenient, and will not affect the behavior of tested organisms in the water body and the living environment. The method can monitor the biological stress response and has the advantages of green, environment-friendliness, realtime online detection, agility, and reliability.

The invention discloses application of protein WRKY78 to regulation of the biological stress resistance of plants. An amino acid sequence of the protein WRKY78 is as shown in sequence 2 in a sequencetable. The expression quantity and/or activity of the protein WRKY78 in oryza sativa L. spp. japonica are improved to obtain transgenic rice A; compared with the oryza sativa L. spp. japonica, the transgenic rice A is significantly improved both in rice stripe disease resistance and rice blast resistance; the expression quantity and/or activity of the protein WRKY78 in the oryza sativa L. spp. japonica are inhibited to obtain transgenic rice B; and compared with the oryza sativa L. spp. japonica, the transgenic rice B is significantly reduced in the aspects of rice stripe disease resistance, rice blast resistance and laodelphax striatellus resistance. Thus it can be seen that the protein WRKY78 can regulate the biological stress resistance of the plants. The protein WRKY78 has important application value.

The invention discloses an induced promoter comprising the following DNA molecules of (1) or (2) or (3): (1) a DNA molecule shown in a sequence 1 in a sequence table; (2) a DNA molecule which is hybridized with the DNA sequence confined by (1) under the strict condition and has the function of the promoter; and (3) a DNA molecule which has more than 90% homology with the DNA sequence confined by (1) or (2) and has the function of the promoter. The invention discloses the promoter PRO-TaFbox2 sequence of the relevant haloduric gene TaFbox2 of wheat, and the overall length of the nucleotide sequence of the promoter is 3076bp. The 2kb zone of the promoter is analyzed and confirmed to contain a plurality of transcription element binding sites relevant to biological stress induction and non-biological stress induction, which shows that a downstream gene controlled by the promoter is bound to be expressed by the stress induction. The induced promoter has very good application prospect in the biological engineering by serving as the induced expression vector of a plant source.

The invention discloses an adversity regulation gene HsCBL8 for improving anti-adversity characteristics of crops. The adversity regulation gene HsCBL8 has a sequence shown in a sequence table SEQ ID No. 1. Correspondingly, the invention discloses a cloning method of the gene. The adversity regulation gene HsCBL8 disclosed by the invention is an upstream gene of a biological and non-biological stress response route of the various crops, can be used for regulating the stress reaction of various adversities, and is significantly beneficial for the anti-adversity breeding research of the crops.

The invention belongs to the field of gene clone in molecular biology, and specifically relates to a hyperaccumulator solanum nigrum metallothionein gene (MT-L3) sequence and a cloning method thereof. The hyperaccumulator solanum nigrum metallothionein gene sequence is type 3 metallothionein gene, and the gene sequence is shown by a nucleotide sequence in SEQ ID NO. 1. The successive cloning and sequencing of the hyperaccumulator solanum nigrum type 3 metallothionein gene helps to establish a base for further analyzing the biological stress resistant capability under the influence of MT-L3, and screening and applying other functions of MT-L3.