High-expression-activity rice non-seed expression promoter SAFES4 and application thereof

A promoter, non-seed technology, applied in the fields of biotechnology and crop genetic engineering, can solve problems such as drug resistance enhancement, ecological balance destruction, non-target biological hazards, etc.

Active Publication Date: 2016-05-11
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Specifically, in terms of ecological environment safety, the problems brought by transgenic rice to the environment are mainly the potential dangers to non-target organisms such as cultivated rice, wild rice, and

Method used

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  • High-expression-activity rice non-seed expression promoter SAFES4 and application thereof
  • High-expression-activity rice non-seed expression promoter SAFES4 and application thereof
  • High-expression-activity rice non-seed expression promoter SAFES4 and application thereof

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Experimental program
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Embodiment Construction

[0039] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0040] The experimental methods in the following examples are conventional methods unless otherwise specified. The biochemical reagents, carrier consumables, etc. used in the following examples are all commercially available products unless otherwise specified.

[0041] Acquisition of SAFES4 Promoter Containing Restriction Sites

[0042] Step 1. Design of primers

[0043] According to the whole genome sequence of rice variety Nipponbare (Oryzasativa Lcv. Nipponbare) provided by NCBI, the amplification primers were designed according to the sequence of rice SAFES4 gene, and the enzyme cutting sites of the primers were designed according to the characteristics of the selected vector and target gene.

[0044] In thi...

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Abstract

The invention provides a high-expression-activity rice non-seed expression promoter SAFES4 and application thereof. The invention also provides an expression box and a crop expression vector, wherein the expression box contains the promoter. Concretely, the promoter SAFES4 provided by the invention can be used for regulation and control by aiming at the defect that foreign genes can realize the concentrated expression in the plants but cannot express in embryon and endosperm. The promoter is used for performing gene modification on the crop varieties; the integral growth features and the mechanism of the rice can be improved; the partial foreign gene expression of the eating part cannot be caused, so that the practical and effective transgene plant varieties can be cultured; the influence on the human eating part can be reduced; the misdoubt of people on the transgene food can be reduced or even eliminated. The promoter provided by the invention can specially drive the foreign genes to be expressed in roots/flowers/green tissues/non-seed tissues; the defect that the green tissue specific expression promoter cannot generally meet the root expression requirements is effectively overcome; great competitive power is realized in the biological stress resistance property improvement.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and crop genetic engineering. Specifically, the present invention relates to a rice non-seed expression promoter with strong expression activity and its application. The promoter can drive the specific expression of target genes in non-seed parts such as roots, flowers and green tissues, so that The purpose of improving rice production performance is achieved under the condition of edible safety and reproductive activity of seeds. Background technique [0002] Genetic modification is the artificial method of changing the genetic arrangement of a species, usually involving the separation of a gene of one organism from a series of genes and implanting it in another organism. Rice is one of the most important food crops in the world. Scientists have carried out in-depth and meticulous research on its genetic characteristics, genetic transformation and breeding utilization, and have also esta...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/82C12N15/84A01H5/00
CPCC07K14/415C12N15/8205C12N15/8223C12N15/8227
Inventor 魏鹏程杨剑波李莉李浩李娟许蓉芳杨亚春秦瑞英
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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