High-expression-activity rice non-seed expression promoter SAFES4 and application thereof

Abstract

The invention provides a high-expression-activity rice non-seed expression promoter SAFES4 and application thereof. The invention also provides an expression box and a crop expression vector, wherein the expression box contains the promoter. Concretely, the promoter SAFES4 provided by the invention can be used for regulation and control by aiming at the defect that foreign genes can realize the concentrated expression in the plants but cannot express in embryon and endosperm. The promoter is used for performing gene modification on the crop varieties; the integral growth features and the mechanism of the rice can be improved; the partial foreign gene expression of the eating part cannot be caused, so that the practical and effective transgene plant varieties can be cultured; the influence on the human eating part can be reduced; the misdoubt of people on the transgene food can be reduced or even eliminated. The promoter provided by the invention can specially drive the foreign genes to be expressed in roots / flowers / green tissues / non-seed tissues; the defect that the green tissue specific expression promoter cannot generally meet the root expression requirements is effectively overcome; great competitive power is realized in the biological stress resistance property improvement.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and crop genetic engineering. Specifically, the present invention relates to a rice non-seed expression promoter with strong expression activity and its application. The promoter can drive the specific expression of target genes in non-seed parts such as roots, flowers and green tissues, so that The purpose of improving rice production performance is achieved under the condition of edible safety and reproductive activity of seeds. Background technique [0002] Genetic modification is the artificial method of changing the genetic arrangement of a species, usually involving the separation of a gene of one organism from a series of genes and implanting it in another organism. Rice is one of the most important food crops in the world. Scientists have carried out in-depth and meticulous research on its genetic characteristics, genetic transformation and breeding utilization, and have also esta...

AI Technical Summary

Problems solved by technology

Specifically, in terms of ecological environment safety, the problems brought by transgenic rice to the environment are mainly the potential dangers to non-target organisms such as cultivated rice, wild rice, and rice field weeds, such as the survival competitiveness of crops, The transfer of target genes, the enhancement of drug resistance, and the harm to non-target organisms, thereby destroying the ecological balance

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