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Litopenaeus vannamei sodium-calcium exchanger LvNCX gene and coding protein thereof

A technology of sodium-calcium exchange and gene coding, applied in the field of genetic engineering, can solve the problems of few reports on NCX gene screening, NCX gene identification, expression analysis and clustering research, etc.

Inactive Publication Date: 2014-03-05
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

Sodium-calcium exchangers, as important ion channel genes, have great application potential in the genetic breeding of Litopenaeus vannamei, but there are few reports on the screening of NCX genes in Litopenaeus vannamei, and the identification, expression analysis and clustering of NCX genes so far no report

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  • Litopenaeus vannamei sodium-calcium exchanger LvNCX gene and coding protein thereof
  • Litopenaeus vannamei sodium-calcium exchanger LvNCX gene and coding protein thereof
  • Litopenaeus vannamei sodium-calcium exchanger LvNCX gene and coding protein thereof

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Embodiment 1

[0029] 1. Cloning and sequencing of the sodium-calcium exchanger gene LvNCX of Litopenaeus vannamei

[0030] 1. Design of primers

[0031] Taking the individual after freshwater stress for 6 hours as Tester and the individual bred at salinity 30 as Driver, a reduced cDNA library related to salinity resistance traits was constructed, and 24 colonies were randomly selected from the library for PCR identification, and the length of the inserted fragment was between 200 ~599bp. The capacity of the library was 2500 clones, and 500 clones were randomly selected for sequencing analysis. A total of 325 high-quality expressed sequence tags (ESTs) and 275 Unigenes (104 Contags and 171 Singletons) were obtained. BLASTx and BLASTn homology comparison with GenBank database, 79.1% of which are known functional genes, genes related to salinity resistance traits include Na-Ca exchange, 5' and 3' RACE primers were designed according to EST sequence, Primers were synthesized by Yingwei Jieji ...

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Abstract

The invention discloses a litopenaeus vannamei sodium-calcium exchanger LvNCX gene and a coding protein thereof. A nucleotide sequence of the litopenaeus vannamei sodium-calcium exchanger LvNCX gene is as shown in SEQ ID NO.1; and an amino acid sequence of the coded sodium-calcium exchanger LvNCX protein is as shown in SEQ ID NO.2. According to the invention, sodium-calcium exchanger LvNCX gene is firstly cloned out from the litopenaeus vannamei, and the gene is proved to be capable of sustaining equilibrium of sodium and calcium ions in a cell body by virtue of experiment, so that theoretical basis is laid for further deeply analyzing biological stress-resistance ability under NCX (Sodium-Calcium Exchanger) influences and applying the NCX gene in stress-resistance research of the litopenaeus vannamei.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the LvVCX gene of the Litopenaeus vannamei sodium-calcium exchanger and its coded protein. Background technique [0002] Sodium / calcium exchanger (NCX) plays an important role in maintaining the balance of sodium and calcium ions in cells. It is a reversible membrane transporter. NCX is found on the cell membrane or the membrane of organelles , whose function is to combine 2 Na + Cells are removed, and 1 calcium ion is brought into the cell, or it can be done in the opposite direction. The function of NCX is to excrete calcium ions in a ratio of 4:1:1 (Na + / Ga2 + / K + ) is discharged in proportion, so it is Na + / K + Dependence is an important channel for maintaining the stability of intracellular ions. [0003] Litopenaeus vannamei (Litopenaeus vannamei), also known as white-footed shrimp, Penaeus vannamei, Pacific white shrimp, commonly known as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/435
CPCC07K14/43509
Inventor 王艳红任春华胡超群张吕平罗鹏
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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