Method for detecting single nucleotide polymorphisms of cattle 17HSDB8 gene

A single nucleotide polymorphism, scalper technology, applied in the field of molecular genetics, can solve problems such as no research reports yet

Inactive Publication Date: 2015-01-14
XINYANG NORMAL UNIVERSITY
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Problems solved by technology

[0007] However, the vast majority of studies on the 17HSDB8 gene are carried out on mo

Method used

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  • Method for detecting single nucleotide polymorphisms of cattle 17HSDB8 gene
  • Method for detecting single nucleotide polymorphisms of cattle 17HSDB8 gene
  • Method for detecting single nucleotide polymorphisms of cattle 17HSDB8 gene

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Embodiment Construction

[0028]The invention detects the single nucleotide polymorphism of the 153rd mutation of the cattle 17HSDB8 gene, so as to be used for marker-assisted selection of growth traits of Chinese yellow cattle, and quickly establish a cattle population with excellent genetic resources. In the following, the present invention will be further described in detail in combination with the detection of specific samples and the association of traits, which is an explanation of the present invention rather than a limitation.

[0029] a, the main reagent sources used in the following examples

[0030] 1. Biochemical reagents and biological reagents

[0031] ①Taq DNA polymerase (purchased from Beijing Tiangen Biochemical Technology Co., Ltd.);

[0032] ②2×buffer++ , dNTPs, etc.>(Beijing Tiangen Biochemical Technology Co., Ltd. Mix)

[0033] ③ restriction endonuclease Sma I (purchased from TAKARA company);

[0034] ④ Proteinase K (purchased from Huamei Bioengineering Company);

[0035] ⑤Mark...

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Abstract

The invention discloses a method for detecting the single nucleotide polymorphisms of a cattle 17HSDB8 gene. The method comprises the following steps: by taking a 17HSDB8 gene-containing cattle genome DNA to be detected as a template and P as a primer, amplifying the cattle 17HSDB8 gene by using a PCR method, then digesting a PCR amplification product by using restriction endonuclease Sma I, then performing polyacrylamide gel electrophoresis to the enzyme-digested segments, and identifying the single nucleotide polymorphisms at 153th locus of the cattle 17HSDB8 gene according to the electrophoresis result. The 17HSDB8 gene has important biological functions on the growth and development characters, and a basis is established for association of the SNP of the gene with the growth character. The single nucleotide polymorphisms of the cattle 17HSDB8 gene has remarkable effect on the early-stage body weight, standing height, body length, chest girth and daily weight gain of a cattle, and can be used as the molecular marker-assisted selection of cattle beef and growth characters so as to further fast establish a cattle population with excellent genetic resources.

Description

technical field [0001] The invention belongs to the field of molecular genetics, relates to the detection of gene single nucleotide polymorphism (Single Nucleotide Polymorphism, SNP), in particular to a method for detecting the 153rd single nucleotide polymorphism of cattle 17HSDB8 gene. Background technique [0002] Gene polymorphism refers to the difference in genome sequence between different species or different individuals within the same species. These differences are caused by nucleotide changes in DNA alleles in chromosomes, mainly including base substitutions, insertions, deletions, and repetitive sequences. Copy number variation. SNP is a type of genetic marker system proposed by Lander (1996), a scholar at the Human Genome Research Center of the Massachusetts Institute of Technology, which refers to the change of a single nucleotide (A / T / C / G) in the genomic DNA sequence caused by polymorphism. Its variant forms include: transversion, conversion, insertion and de...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 马云陈宁博高雪李芬韩雅彭常艳林蒋祥祥
Owner XINYANG NORMAL UNIVERSITY
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