Method for detecting single nucleotide polymorphisms of cattle 17HSDB8 gene

Abstract

The invention discloses a method for detecting the single nucleotide polymorphisms of a cattle 17HSDB8 gene. The method comprises the following steps: by taking a 17HSDB8 gene-containing cattle genome DNA to be detected as a template and P as a primer, amplifying the cattle 17HSDB8 gene by using a PCR method, then digesting a PCR amplification product by using restriction endonuclease Sma I, then performing polyacrylamide gel electrophoresis to the enzyme-digested segments, and identifying the single nucleotide polymorphisms at 153th locus of the cattle 17HSDB8 gene according to the electrophoresis result. The 17HSDB8 gene has important biological functions on the growth and development characters, and a basis is established for association of the SNP of the gene with the growth character. The single nucleotide polymorphisms of the cattle 17HSDB8 gene has remarkable effect on the early-stage body weight, standing height, body length, chest girth and daily weight gain of a cattle, and can be used as the molecular marker-assisted selection of cattle beef and growth characters so as to further fast establish a cattle population with excellent genetic resources.

Description

technical field [0001] The invention belongs to the field of molecular genetics, relates to the detection of gene single nucleotide polymorphism (Single Nucleotide Polymorphism, SNP), in particular to a method for detecting the 153rd single nucleotide polymorphism of cattle 17HSDB8 gene. Background technique [0002] Gene polymorphism refers to the difference in genome sequence between different species or different individuals within the same species. These differences are caused by nucleotide changes in DNA alleles in chromosomes, mainly including base substitutions, insertions, deletions, and repetitive sequences. Copy number variation. SNP is a type of genetic marker system proposed by Lander (1996), a scholar at the Human Genome Research Center of the Massachusetts Institute of Technology, which refers to the change of a single nucleotide (A / T / C / G) in the genomic DNA sequence caused by polymorphism. Its variant forms include: transversion, conversion, insertion and de...

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Problems solved by technology

[0007] However, the vast majority of studies on the 17HSDB8 gene are carried out on model animals such as humans and mice, and studies on cattle have not been reported yet.

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