Method for preventing dendrobium officinale tissue culture seedlings from being polluted by bacteria

A technology of Dendrobium officinale and bacterial contamination, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve problems such as bacterial contamination, and achieve the effects of reducing damage, reducing bacterial contamination rate and easy operation.

Pending Publication Date: 2021-01-19
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The seeds exposed after the pod cracking of Dendrobium officinale are still prone to bacterial contamination after aseptic treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for blocking dendrobium officinale tissue culture plantlets from being polluted by bacteria, characterized in that it comprises the following steps:

[0021] 1) Preparation of nano-copper mother liquor, put nano-copper into sterilized distilled water, ice bath at 40 ° C for 40 minutes, and then put it in an ultrasonic cell disruptor for 40 minutes under the ice bath condition, and obtain a mass concentration of 0.1% nano-copper mother liquor;

[0022] 2) Dilute the nano-copper mother liquor, add sterilized distilled water to the nano-copper mother liquor in step 1), and dilute to a mass concentration of 10 -2 % of the nano-copper solution, sent into the oven, heated to 70 ° C and then kept warm to maintain the activity of the nano-copper;

[0023] 3) Preparation of disinfectant solution, adding Ujie 1003 disinfectant solution to sterile distilled water to a mass concentration of 0.2%, autoclaving at 125°C and 0.1Mpa for 20 minutes to obtain a disinfectant solu...

Embodiment 2

[0033] A method for blocking dendrobium officinale tissue culture plantlets from being polluted by bacteria, characterized in that it comprises the following steps:

[0034] 1) Preparation of nano-copper mother liquor, put nano-copper into sterilized distilled water, ice bath at 60 ° C for 20 minutes, and then put it in an ultrasonic cell disruptor for 20 minutes under the ice bath condition, and obtain a mass concentration of 0.1% nano-copper mother liquor;

[0035] 2) Dilute the nano-copper mother liquor, add sterilized distilled water to the nano-copper mother liquor in step 1), and dilute to a mass concentration of 10 -2 % of nano-copper solution, sent into the oven, heated to 60 ° C and then kept warm to maintain the activity of nano-copper;

[0036] 3) Preparation of disinfectant solution, adding Ujie 1003 disinfectant solution to sterile distilled water to a mass concentration of 0.2%, autoclaving at 110°C and 0.1Mpa for 30 minutes to obtain a disinfectant solution;

...

Embodiment 3

[0046] A method for blocking dendrobium officinale tissue culture plantlets from being polluted by bacteria, characterized in that it comprises the following steps:

[0047] 1) Preparation of nano-copper mother liquor, put nano-copper into sterilized distilled water, ice bath at 50 ° C for 30 minutes, and then put it in an ultrasonic cell disruptor for 30 minutes under the ice bath condition, and obtain a mass concentration of 0.1% nano-copper mother liquor;

[0048] 2) Dilute the nano-copper mother liquor, add sterilized distilled water to the nano-copper mother liquor in step 1), and dilute to a mass concentration of 10 -2 % of the nano-copper solution, sent into the oven, heated to 65 ° C and then kept warm to maintain the activity of nano-copper;

[0049] 3) Preparation of disinfectant solution, adding Ujie 1003 disinfectant solution to sterile distilled water to a mass concentration of 0.2%, autoclaving at 120°C and 0.1Mpa for 25 minutes to obtain a disinfectant solution...

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PUM

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Abstract

The invention provides a method for preventing dendrobium officinale tissue culture seedlings from being polluted by bacteria. The method is characterized by comprising the following steps of (1) preparing a nano-copper mother solution; (2) diluting the nano-copper mother solution; (3) preparing a disinfectant; (4) preparing a mixed culture medium I; (5) disinfecting dendrobium officinale seeds; (6), culturing the dendrobium officinale seeds; (7), performing differentiation culture on the dendrobium officinale seeds; (8), preparing a mixed culture medium II; (9), culturing differentiation immature embryos; and (10), culturing and transplanting bottle seedlings. The nano-copper solution with the concentration of 10<-2>% is added into the culture media, infection bacteria are blocked in theseed germination culture process and the immature embryo differentiation culture process, the bacterial contamination rate of dendrobium officinale is effectively reduced, the unique technical effectis achieved, and the blocking rate is as high as 93%. Damage of antibiotics to plants can be reduced, the survival rate of dendrobium officinale seedlings can be increased, even if pollution exists, blocking can be achieved, and therefore a large number of experimental materials can be preserved.

Description

technical field [0001] The invention relates to the field of new materials and plant tissue culture, in particular to a method for blocking bacterial contamination of dendrobium candidum tissue culture seedlings by using nanometer copper. Background technique [0002] Nanomaterials with one-dimensional, two-dimensional or three-dimensional dimensions of only a few nanometers or tens of nanometers are made by advanced physical, chemical, biological and other technologies, and their structures are very different from ordinary materials, such as nanograin boundaries. The atomic spacing is large, the density is low, and the atomic arrangement is random. In recent years, related research on the use of nano-copper for antibacterial has gradually attracted attention. Due to its quantum effect, small size effect, and large surface area, nano-copper exhibits better safety, antibacterial properties, and long-term antibacterial properties. [0003] The exposed seeds after pod crackin...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 李涵曹桦陆琳姬语璐李绅崇张颢
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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