436 results about "Functional genes" patented technology

The invention relates to the field of microbial communities, and discloses a bioinformatics-based microbial community dynamic monitoring method, which comprises the following steps: acquiring high-frequency acquisition data based on a trace sample, constructing a microbial data stream for time sequence analysis, and carrying out rapid metagenome marker amplification on each sampling unit in the data stream, obtaining a preliminary feature matrix; based on the co-occurrence frequency of the microbial functional genes in the preliminary feature matrix, constructing a multi-dimensional feature mapping graph; performing real-time mode recognition on the flora abundance change trend based on the dynamic fluctuation region; aiming at the key dynamic signal segment, adopting a distributed clustering method based on variation information entropy regulation and control to reconstruct the evolution trajectory of the flora, and generating a flora time sequence behavior vector set; and based on the flora time sequence behavior vector set, performing dynamic alignment with a pre-constructed reference model by using a multi-scale trend matching algorithm. The method has the advantages of high timeliness and automatic processing capability.

The invention provides a Ustilaginoidea virens gene UvASE2 for regulating and controlling rice resistance and application of the Ustilaginoidea virens gene UvASE2, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the Ustilaginoidea virens gene UvASE2 is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the Ustilaginoidea virens gene UvASE2 is as shown in SEQ ID NO: 2. By constructing a transgenic rice plant for heterologous expression of UvASE2, it is found that the transgenic rice can significantly improve the resistance to false smut and bacterial blight and significantly induce the outbreak of active oxygen. The heterologous expression UvASE2 gene of Ustilaginoidea virens has the function of positively regulating and controlling the disease resistance of rice, and the UvASE2 gene can be used for improving the disease resistance of rice and has important significance on creation of disease-resistant germplasm of rice.

The invention discloses an application of a rape BnC05ERF378 gene in improving the waterlogging resistance of a plant. The nucleotide sequence of the rape BnC05ERF378 gene is as shown in SEQ ID NO. 1. According to the invention, a brand new stain-resistant gene BnC05ERF378 is cloned from rape for the first time, and is transferred into arabidopsis thaliana to construct a pure line plant and complete stain resistance identification. Experimental results show that the growth state of the transgenic BnC05ERF378 gene arabidopsis thaliana is remarkably superior to that of a wild type after waterlogging treatment, the transgenic BnC05ERF378 gene arabidopsis thaliana can normally bolt, the survival rate is increased from 12.5% to 37.5%, it is fully proved that the gene can enhance the survivability of the plant in a waterlogging environment by regulating the response mechanism of the plant to waterlogging stress, and the survival rate of the plant is increased from 12.5% to 37.5%. And a key guarantee is provided for stable growth of plants under high-humidity, waterflooding and other adverse conditions. According to the discovery and application of the waterlogging-resistant gene BnC05ERF378 disclosed by the invention, a clear functional gene target is provided for improvement of waterlogging resistance of crops. By constructing an expression vector containing the gene and combining mature technologies such as agrobacterium transformation, the gene can be stably introduced into a target plant, and a pure transgenic plant is obtained.

The invention provides application of a soybean GmWRKY40 gene in soybean breeding, and belongs to the technical field of functional gene breeding. The nucleotide sequence of the soybean GmWRKY40 gene provided by the invention is as shown in SEQ ID NO: 1. According to the invention, the GmWRKY40 gene is constructed into a skeleton expression vector to obtain a recombinant vector, and the recombinant vector is transferred into soybean under the mediation of agrobacterium tumefaciens to obtain a transgenic plant with stable overexpression of the GmWRKY40 gene. Detection results show that overexpression of the GmWRKY40 gene can significantly improve the grain isoflavone content and the protein oil content of the transgenic soybean, improve the soybean quality and increase the soybean grain yield at the same time. Therefore, the soybean GmWRKY40 gene can be used for developing new varieties of high-quality soybeans.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention provides application of a Zm00001eb378820 gene in molecular marker-assisted breeding for regulating and controlling the hundred-grain weight of corn grains, and relates to the technical field of agricultural biology. A backbone maize inbred line Ye107 is used as a male parent and hybridized with three excellent inbred lines (D39, R-2-1-1 and YML1218), a multi-parent group with remarkable hundred-grain weight difference is constructed, and SNP27712538 positioned on a chromosome 9 is a remarkable associated site by measuring hundred-grain weight phenotype data in multiple environments and combining high-density SNP (Single Nucleotide Polymorphism) markers, GWAS analysis and QTL (Quantitative Trait Loci) positioning. And the functional gene Zm00001eb378820 which is closely related to the regulation and control of the corn hundred-grain weight is excavated. A new insight is provided for a genetic regulation mechanism of the corn hundred-grain weight, and a key target spot is provided for breeding of high-yield corn varieties, so that the breeding efficiency is remarkably improved.

The invention discloses a construction method of a soil microorganism nitrogen metabolism function prediction model, and relates to the technical field of environmental microbiology and agricultural information, and the method comprises the following steps: S1, constructing a spatial-temporal heterogeneity representation engine, and dynamically decoupling a multi-level gradient of a soil environment through an adaptive spatial-temporal mesh generation algorithm; according to the construction method of the soil microorganism nitrogen metabolism function prediction model, the adaptive capacity of the soil microorganism nitrogen metabolism function prediction model in a complex environment is improved, and multi-level environment gradient dynamic analysis from a centimeter-level rhizosphere microdomain to a kilometer-level landscape scale is realized; the problem of cross-scale feature fusion caused by temporal-spatial resolution mismatch of a traditional model is solved, the simplified hypothesis of a traditional statistical model on the synergistic-antagonistic relationship of microbial functional genes is broken through, a dynamic coupling mechanism of a nitrogen metabolism path under environmental disturbance is accurately quantified, and the prediction error is reduced compared with the prior art.

The invention discloses a crested ibis habitat ecological quality trend analysis method and system, and relates to ecological quality monitoring. The method comprises the following steps: S1, constructing an intelligent sensing network, synchronously obtaining multi-source data of a habitat, identifying activity events of crested ibis, and generating a multi-dimensional habitat parameter table; s2, collecting environmental samples, and generating a microbial functional gene abundance matrix through metagenome sequencing and bioinformatics analysis; s3, taking the activity events of the crested ibis as behavior tags, and generating habitat function health indexes by coupling the parameter table and the matrix training machine learning prediction model; s4, performing spatial interpolation and trend analysis based on the habitat function health index to generate an ecological quality space-time evolution graph; and S5, based on the ecological quality space-time evolution graph, performing quantitative analysis by using a spatial differentiation statistical model, and generating a trend analysis report. By fusing multi-source data, real-time dynamic evaluation of habitat ecological quality and quantitative analysis of driving factors are realized, and a direct decision basis is provided for accurate protection.

The invention belongs to the technical field of molecular marker assisted breeding, particularly relates to a molecular marker related to white spot resistance on a corn chromosome 4 and application, and particularly provides the molecular marker related to the corn white spot resistance, the gene of the molecular marker is a Zm00001eb168510 gene and / or a sequence as shown in SEQ ID NO: 1, the CDs sequence of the Zm00001eb168510 gene is as shown in SEQ ID NO: 2, and the CDs sequence of the Zm00001eb168510 gene is as shown in SEQ ID NO: 1. According to the invention, through many years of multi-site experiments, the white spot resistance QTL qMWS4-4 interval and the resistance SNP: 4-14146725 and the associated gene Zm00001eb168510 thereof are jointly localized on the corn chromosome 4, the SNP locus can be localized in different environments through QTL linkage analysis and GWAS association analysis, and 15.14% of phenotypic variation can be explained; the associated candidate functional gene mainly encodes cysteine protease superfamily protein, and a cysteine protease large family contains deubiquitination enzyme which plays an important role in plant development and stress response.

The invention discloses an application of some functional genes in improving the performance of clostridium for synthesizing ethanol by utilizing one-carbon gas fermentation, wherein overexpression genes NCBI (National Center for Biotechnology Information) are CLJUc04490, CLJUc25060, CLJUc06990, CLJUc22650 and / or CLJUc07720, and expression of inhibition genes CLJUc09160 and / or CLJUc23840 can be used for improving the autotrophic growth capability and the ethanol synthesis yield of clostridium Yangdall when the clostridium Yangdall is fermented by utilizing C1 gas; therefore, the economical efficiency of the clostridium yangdall fermentation is improved.

The invention belongs to the technical field of gene engineering and salt-tolerant plant cultivation, and particularly relates to a wild soybean salt-tolerant gene and application thereof. The gene GsWRKY53 responding to salt stress is identified by performing expression analysis on the gene of the wild soybean under salt treatment, the salt-tolerant gene GsWRKY53 in the wild soybean is transferred into a soybean cotyledon through an agrobacterium transformation method for the first time, and the gene function of the gene can be quickly and accurately verified by verifying the influence of the salt-tolerant gene GsWRKY53 on soybean hairy roots. Experiments prove that the fresh weight, the dry weight and the number of hairy roots of soybeans can be reduced by overexpression of the wild soybean salt-tolerant gene GsWRKY53, which indicates that the GsWRKY53 plays a negative regulation role in the salt tolerance of the soybeans. According to the technical scheme, a foundation is provided for screening and verifying the salt-tolerant functional gene and further screening or cultivating salt-tolerant plant varieties, especially salt-tolerant soybean varieties, so that the method has a good practical application value.

The invention relates to a functional gene abundance-based river nitrogen concentration simulation method, which comprises the following steps of: 1, determining a nitrogen conversion process related to a model according to environmental condition characteristics of a river; 2, determining functional genes and chemical substances involved in the model according to the selected nitrogen conversion process, and obtaining the abundance of the involved functional genes and the concentration of the chemical substances through on-site sampling and monitoring; and step 3, combining the functional gene abundance change equation and the chemical substance concentration change equation, inputting the functional gene abundance and the chemical substance concentration obtained in the step 2 into the equations, and calculating nitrogen concentrations in different spaces and time so as to simulate the nitrogen concentration of the river. According to the method, the mathematical model of the river nitrogen conversion process is constructed through the microbial metabolism reaction mechanism, and compared with a traditional water environment model, the distribution characteristics of nitrogen can be more accurately simulated, and the change rule can be predicted.

The invention provides a corn plant type regulation gene ZmEXO1 and application, and belongs to the technical field of corn molecular breeding. The amino acid sequence of the ZmEXO1 is as shown in SEQ ID NO.7. The research finds that the ZmEXO1 can regulate and control the corn leaf included angle and the plant height, and the corn leaf included angle is reduced and the plant height is reduced due to the fact that a mutant with the expression quantity of the ZmEXO1 reduced or silenced. Therefore, the germplasm capable of improving the corn plant type can be obtained by carrying out gene editing on the ZmEXO1 gene or carrying out genetic breeding improvement by utilizing the Zmeso1 low-expression mutant, and the method has important significance on enriching the corn germplasm and cultivating density-tolerant high-yield varieties. The invention further provides a Kasp molecular marker and a Kasp primer which are closely linked with the ZmEXO1, and the Kasp molecular marker and the Kasp primer can be used for molecular marker-assisted selective breeding and mining of related functional genes and can be used for rapidly screening and identifying ZmEXO1 molecular improvement-containing corn germplasm.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to corn plant height regulation and application thereof. The sequence of the molecular marker is shown as SEQ ID NO.1, the 201bp site basic group of the sequence from the 5th end shows C / T polymorphism, a temperate zone corn inbred line Ye107 with low plant height is used as a common male parent and is respectively hybridized with one temperate zone and four tropical / subtropical corn inbred lines as female parents, and a corn polyparent group with obvious plant height difference is constructed. GWAS analysis and linkage analysis are used for co-localization to Chr1: SNP-25, 296 and 496 which are located in a chromosome 1 and remarkably related to the plant height, 3.59% of plant height phenotypic variation can be explained through the loci, a functional gene for regulating and controlling the corn plant height is excavated from the loci, and a qRT-PCR result shows that the gene is highly expressed in corn V14 period internodes, so that it is proved that the gene participates in regulation and control height correlation of the corn plant height, and the functional gene is a functional gene for regulating and controlling the corn plant height. Results of the invention provide technical support for efficient and accurate breeding of high-stalk or dwarf high-yield corn varieties by molecular markers.

The invention discloses Aspergillus sp. DLF18 and application thereof, and relates to the technical field of microorganisms, and the preservation number of the Aspergillus sp. DLF18 is CGMCC (China General Microbiological Culture Collection Center) NO.41737. The strain can effectively utilize rice straws and / or bagasse to produce humic acid, and the content of humic acid in each gram of fermentation supernatant after the rice straws are fermented is 16.5%. When the DLF18 fermentation product is applied to crops, the growth condition of the crops can be remarkably improved, and the humus content in soil is increased. Meanwhile, a metagenomics analysis result shows that compared with a control group, after the DLF18 is applied into the soil, the species beta diversity is remarkably changed, and the functional gene abundance and the inter-group difference are remarkably changed. Therefore, the DLF18 has a wide application prospect in the aspects of agricultural straw utilization and soil remediation.

The invention relates to the field of corn molecular marker assisted breeding, and particularly discloses an application of a Zm00001eb023590 gene in ear thickness corn breeding, the invention provides a corn ear thickness molecular marker, the sequence of the molecular marker is shown as SEQ ID NO: 1, GWA analysis and genetic linkage analysis are utilized to jointly locate to a chromosome 1 and excavate to a functional gene Zm00001eb023590 for regulating and controlling ear thickness, and the functional gene Zm00001eb023590 for regulating and controlling ear thickness is obtained. According to the present invention, the corn ear thickness character genetic gene is enriched, the representative molecular marker site in the gene is screened by carrying out haplotype analysis on the gene, the redundancy detection cost in molecular breeding is reduced, and the integrity of the genetic information is retained;

Provided are a Medicago sativa SNP molecular marker combination and a use thereof. The SNP molecular marker combination consists of 61351 SNP molecular markers. The physical positions of the 61351 SNP molecular markers are determined by sequence alignment on the basis of the Medicago sativa reference genome ZM-4 alfalfa genome. The SNP molecular markers have good site specificity, strong versatility, and high polymorphism and can effectively discriminate Medicago sativa germplasms from different sources. A Medicago sativa genome-wide liquid-phase gene chip is prepared from the SNP molecular markers, and can be used for genetic diversity assessment of Medicago sativa germplasm resources, genetic map construction and functional gene mapping, genome-wide association study, etc., providing an important tool and technical support for Medicago sativa molecular design breeding and variety authenticity identification, and achieving important significance for high-quality development of the alfalfa seed industry in China.

The invention discloses Penicillium chrysogenum DLS189 and application thereof, and relates to the technical field of microorganisms, and the preservation number of the Penicillium chrysogenum DLS189 is CGMCC (China General Microbiological Culture Collection Center) NO.41739. The invention further discloses a preparation method of the Penicillium chrysogenum DLS189. The strain can effectively utilize rice straws and / or bagasse to produce humic acid, and the content of humic acid in each gram of fermentation supernatant after the rice straws are fermented is 16.84%. When the DLS189 fermentation product is applied to crops, the growth condition of the crops can be remarkably improved, and the humus content in soil is increased. Meanwhile, a metagenomics analysis result shows that compared with a control group, after the DLS189 is applied into the soil, the species beta diversity is remarkably changed, and the functional gene abundance and the inter-group difference are remarkably changed. Therefore, the DLS189 has a wide application prospect in the aspects of agricultural straw utilization and soil remediation.

The invention relates to the field of corn molecular marker assisted breeding, and particularly discloses an application of a Zm00001eb050010 gene in regulation and control of corncob thickness, the invention provides a corncob thickness molecular marker, the molecular marker is a Zm00001eb050010 gene with a sequence shown as SEQ ID NO.1, the molecular marker is used for analyzing SNP255223086 which is located in a chromosome 1 and is remarkably related to the corncob thickness in a plurality of environments by utilizing GWAS analysis, and the molecular marker is a Zm00001eb050010 gene with a sequence shown as SEQ ID NO.3. The molecular marker is a Zm00001eb050010 gene with a sequence shown as SEQ ID NO.4. The molecular marker is a Zm00001eb050010 gene. The functional gene Zm00001eb050010 and SNP255223086 for regulating and controlling the ear thickness can explain 5.23% of the phenotypic variation of the ear thickness, and by means of molecular marker breeding, the generation interval can be shortened, the breeding process can be greatly accelerated, the selection precision can be improved, and the cost can be saved.

The invention belongs to the technical field of soil heavy metal pollution remediation, and discloses a heavy metal pollution soil bioremediation method and system based on soil metagenome sequencing, and the method comprises the following steps: carrying out gene sequencing on experimental microorganism class groups in heavy metal pollution soil to determine functional microorganisms containing heavy metal resistance genes; heavy metal resistant microorganism species in the soil can be accurately identified by judging whether functional genes are contained or not, and ecological unbalance possibly caused by blind introduction of alien species is avoided; a remediation strategy taking a microbial resistance mechanism as a core can effectively reduce the bioavailability of heavy metals in the soil, and also can improve the physicochemical properties of the soil through metabolic activity to form a sustainable remediation effect; the method has the advantages of being environmentally friendly, controllable in cost, lasting in effect and the like, and an innovative path is provided for soil ecological function restoration.

The invention discloses a method for inducing multiple types of somatic cells to change fate and directly develop into somatic embryos and application, and belongs to the technical field of plant genetic engineering. According to the invention, functional genes SPCH (AT5G53210) and ARF5 (AT1G19850) capable of promoting conversion of various types of somatic cells into the totipotent stem cells are found. Overexpression of the totipotent regulatory factor LEC2 can induce formation of somatic embryos on arabidopsis sinapis leaves, under the background, the functional genes are subjected to ectopic expression, more arabidopsis sinapis leaf cells can be converted into totipotent stem cells through SPCH overexpression without calluses, and therefore the totipotent stem cells can be used for inducing formation of the somatic embryos on the arabidopsis sinapis leaves. According to the method, more arabidopsis sinapis leaf cells can be transformed into totipotent stem cells, stem tip cells, hypocotyl cells and root cells can be transformed into totipotent stem cells, more arabidopsis sinapis leaf cells can be transformed into totipotent stem cells through ARF5 overexpression, the stem tip cells and the root cells can be transformed into totipotent stem cells, and finally somatic embryos are formed through induction.

The invention relates to the technical field of functional gene and protein engineering, and discloses an amino acid sequence, a gene sequence, an expression vector and application of medicago ruthenica MrAGL8 protein, the amino acid sequence of the medicago ruthenica MrAGL8 protein is as shown in SEQ ID NO.1, the gene sequence for coding the medicago ruthenica MrAGL8 protein is as shown in SEQ ID NO.2, and the gene sequence for coding the medicago ruthenica MrAGL8 protein is as shown in SEQ ID NO.1. The invention further discloses application of the medicago ruthenica MrAGL8 protein to construction of an early-maturing plant strain or a low-pod-cracking-rate plant strain. Experiments show that the MrAGL8 gene plays an important role in promoting the early maturing of plants and reducing the pod cracking rate, and by utilizing the phenomenon that the found MrAGL8 gene participates in the response reaction of plant growth and development and pod cracking, the MrAGL8 gene is used for constructing an early maturing plant strain or a low pod cracking rate plant strain and carrying out heterologous overexpression; therefore, early maturing of transgenic plants is effectively promoted, and the pod cracking rate is remarkably reduced.

The invention discloses a pennisetum purpureum CpERF37 transcription factor and cold-resistant application thereof, and relates to the technical field of plant genetic engineering. According to the invention, the Pennisetum purpureum-sourced ERF transcription factor CpERF37 is cloned and functionally identified for the first time, and heterologous expression verification in a model plant arabidopsis thaliana proves that the Pennisetum purpureum-sourced ERF transcription factor CpERF37 has a remarkable effect on improving the low-temperature tolerance of plants, and functional gene resources related to low-temperature stress response are enriched. The method not only has theoretical innovation, but also has a definite application prospect, and can provide effective gene resources and technical support for cold-resistant improvement of pasture and crops.

The invention relates to a gene ZmILR1 for controlling the size of a corn kernel as well as a molecular identification method and application of the gene ZmILR1, and belongs to the technical field of plant genetic breeding and functional gene research. The ZmILR1 gene encodes an IAA-amino acid hydrolase, mutation of the IAA-amino acid hydrolase causes corn kernels to become smaller, and overexpression of the gene can create a corn material with increased kernel length, kernel width and hundred-kernel weight. A functional molecular marker A1A4 is developed according to the sequence difference of the mutant gene and a wild type gene at the DNA level, and can be used for rapid identification and screening of small-grain corn materials. The invention provides a theoretical basis and an application tool for analyzing a molecular mechanism of the corn grain size and developing molecular marker-assisted breeding and hybrid seed breeding, and provides important gene resources and technical support for high-yield and high-quality molecular breeding of corn.

The invention discloses a tobacco black shank-resistant G24 gene and application thereof in disease-resistant variety cultivation, and belongs to the technical field of tobacco molecular biology and genetic breeding. The nucleotide sequence of the G24 gene is as shown in SEQ ID NO: 1 or has the homology greater than or equal to 95% with the SEQ ID NO: 1; after the expression of the G24 gene is inhibited or the G24 gene is knocked out, tobacco with black shank resistance can lose the black shank resistance, and infected tobacco can obtain the black shank resistance by over-expression of the G24 gene. Based on the gene, the tobacco variety cultivated by the invention has high resistance to the phytophthora parasitica var nicotianae No. 0 microspecies, has excellent agronomic and quality characters, can be used for preparing tobacco products such as cigarettes and the like, and reduces the use of bactericides. The method solves the problems of undefined functional genes and low selection efficiency in existing black shank resistant breeding, and provides a key technology for green prevention and control of tobacco black shank.

The invention relates to an agrobacterium tumefaciens-mediated cork tree genetic transformation method. The method comprises the following steps: preparing an infection solution from an agrobacterium solution; the method comprises the following steps: taking in-vitro cotyledons or stems as explants after cork tree seeds germinate into seedlings, infecting the cotyledons or stems of the cork tree by adopting an infection solution, and carrying out co-culture, recovery culture and screening culture after infection is completed, so as to obtain transgenic hairy roots or transgenic calluses. According to the scheme provided by the invention, stable transformation of balsa roots can be realized, verification of functional genes is realized, the functional genes exist in a living body form for a long time, and the genetic transformation efficiency of balsa is improved.

The invention provides an agrobacterium rhizogenes-mediated forsythia suspensa hairy root genetic transformation method and application thereof, and belongs to the technical field of plant genetic engineering. The method comprises the following steps: introducing an expression vector into agrobacterium rhizogenes, and mixing with an infection solution to prepare an agrobacterium rhizogenes infection solution; the forsythia suspensa stem is put into the agrobacterium rhizogenes infection liquid to be infected for 0.5-1 h through a vacuum infiltration method, and the infected forsythia suspensa stem is cut into a matrix to be subjected to dark culture and then continues to grow till hairy roots are generated; the OD600 value of the agrobacterium rhizogenes infection liquid is 0.5 to 1.0. The method is suitable for an agrobacterium rhizogenes mediated genetic transformation system of the fructus forsythiae, and the system provides technical support for functional gene research, medicinal component analysis and molecular breeding of the fructus forsythiae, and has important significance for promoting genetic improvement and resource utilization of the fructus forsythiae.

The invention belongs to the technical field of gene editing, and particularly relates to a construction method and application of a needle mushroom CRISPR / Cas9 gene editing vector. The CRISPR / Cas9 gene editing vector for flammulina velutipes is characterized in that the vector takes pFgnpt as a skeleton vector, and further comprises a Cas9 expression cassette and an sgRNA expression cassette; the nucleotide sequence of the Cas9 expression cassette is as shown in SEQ ID NO. 10. The nucleotide sequence of the sgRNA expression cassette is as shown in SEQ ID NO. 11. According to the present invention, with the agrobacterium-mediated transformation technology, the stable expression of Cas9 and sgRNA in the flammulina velutipes body is achieved, the efficient and stable CRISPR / Cas9 gene editing system is established, and the technical support is provided for the flammulina velutipes variety improvement, the functional gene research and the industrial upgrading.

The invention provides a plant cytosine single-base editor as well as a construction method and application thereof, and relates to the technical field of plant genetic engineering. The single-base editor provided by the invention is named as hyPopCBE, and has a structural formula shown in the description, the editor comprises hA3A / Y130F cytosine deaminase, an optimized human-derived Rab51 single-stranded DNA binding protein structural domain, a fusion protein of nCas9 nuclease and a uracil glycosylase inhibitor, a fusion protein expression unit of phage MS2 coat protein and the uracil glycosylase inhibitor, and a guide RNA transcription expression unit integrated with an MS2 binding sequence. Compared with an existing plant cytosine single-base editor, the single-base editor constructed by the method can remarkably improve the editing efficiency of plant alleles, and the stability is higher; when the herbicide-resistant poplar gene is applied to poplar, a new herbicide-resistant poplar germplasm can be successfully created, and a technical support is provided for research and molecular breeding of a poplar functional genome.

The invention discloses a polyamine transporter encoding gene HvPUT5 derived from highland barley and application of the polyamine transporter encoding gene HvPUT5 in improvement of cell osmotic stress tolerance, and relates to the technical field of plant stress resistance molecular breeding and functional gene mining. The HvPUT5 is positioned in cytoplasm, and the over-expression of the gene in a yeast system can obviously improve the survival rate of the yeast under the condition of high osmotic pressure, which indicates that the gene has a positive effect on enhancing the osmotic stress tolerance. The HvPUT5 has a good application prospect, can be used as a candidate gene resource and a molecular marker for drought-resistant breeding, and is applied to genetic improvement and molecular breeding research of crop stress resistance characters.