93 results about "Larimichthys crocea" patented technology

The invention discloses a large model-based large yellow croaker growth stage feeding amount evaluation method and system, and belongs to the field of aquaculture, and the method comprises the steps: collecting underwater images of a fish pond in a plurality of shooting directions at the same time, and recognizing and segmenting a fish body image corresponding to each entity fish in each underwater image; determining the distance between an entity fish corresponding to the fish body image and a camera for collecting the fish body image, and extracting fish body appearance features of the fish body image; randomly selecting a fish body image from each shooting direction to form a fish body image association combination, and screening a second fish body image association combination from all possible fish body image association combinations according to the distance and fish body appearance features; and constructing three-dimensional point cloud data of the entity fishes according to the second fish body image association combination, fitting three-dimensional body shape parameters of the entity fishes through the three-dimensional point cloud data, and determining the final feeding amount of the fish school according to the three-dimensional body shape parameters of the entity fishes. According to the invention, the problem of low accuracy of the real-time feeding amount of the fish school can be solved.

The invention discloses a large yellow croaker feeding behavior monitoring method and system based on target detection, and the method comprises the steps: carrying out the feature extraction of a large yellow croaker feeding video collected in real time, obtaining a visual behavior feature, and carrying out the feature extraction of a large yellow croaker acoustic signal collected in real time, and obtaining an acoustic behavior feature; performing time alignment on the visual behavior features and the acoustic behavior features through a preset cross-modal phase synchronization network to obtain a first feature pair, and performing semantic alignment on the first feature pair through a preset attention mechanism to obtain a second feature pair; performing feature fusion on the second feature pair to obtain a multi-modal feature, and outputting an ingestion behavior recognition result according to the multi-modal feature through a preset first ingestion behavior recognition model; and when the ingestion behavior result belongs to the abnormal ingestion behavior category, performing ingestion behavior abnormal early warning according to a preset grading early warning mechanism. The accuracy of large yellow croaker feeding behavior monitoring can be improved.

This invention discloses a device and method for the propagation, release, and rewilding training of large yellow croaker fry, relating to the field of marine biological resource conservation technology. The device includes: a water tank containing water, with multiple artificial reefs placed inside; a light source positioned above the water tank; multiple wave-making pumps evenly distributed along one side of the inner wall of the water tank; a release structure for releasing predator pheromones into the water tank; a camera mounted on the inner wall of the water tank; and a controller electrically connected to the camera, light source, and wave-making pumps. The camera collects behavioral characteristic information of the large yellow croaker during rewilding training and sends this information to the controller. A fish behavior analysis system comprehensively analyzes the swimming and reef usage of the large yellow croaker fry in the water tank to determine the effectiveness of the rewilding training. The advantages of this invention are enhanced ability of large yellow croaker fry to adapt to the marine environment and improved survival rate of released fry.

This invention discloses a multi-unit composite stress tiered breeding device and method for juvenile large yellow croaker, belonging to the field of aquaculture breeding technology. The device uses a central control unit as its sole core, forming a fully integrated unidirectional closed-loop drive system. Four independent breeding units are connected through a transparent channel with a centrally located aeration head, forming a physical flow-limiting isolation structure. Each unit independently regulates water temperature, salinity, and dissolved oxygen, creating a multi-factor composite stress gradient. The breeding method employs a three-factor linear equal-gradient synergistic progressive setting, utilizing the juvenile fish's autonomous adaptation and avoidance instincts to achieve cross-unit migration and natural selection. Combined with contour recognition and dynamic tracking algorithms, it monitors the fish population status and mortality rate in real time, and identifies superior offspring with comprehensive stress resistance through secondary verification under extreme stress. This invention offers high screening accuracy and automation, increasing the survival rate of superior individuals by over 40%, reducing labor costs by over 60%, and allowing for flexible adjustment of device parameters. It can be extended to the stress resistance breeding of other marine economic fish species.

The application discloses a method for quantitatively analyzing the infection degree of white spots in the spleen of Pseudosciaena crocea based on machine learning, and relates to a threshold segmentation technology of machine learning. 1) Data acquisition: dissect the Pseudosciaena crocea infected with internal visceral white spot disease, use a high-definition camera to shoot the spleen, and acquire pictures of the white spots in the spleen to be quantitatively analyzed; 2) creation of an interactive segmentation GUI interface: used for reading the pictures of the white spots in the spleen of the Pseudosciaena crocea and automatically converting the pictures into binary images; 3) running of a main program: the main program is written by a python code and is used for the GUI interface to display the selected pictures on the interactive segmentation GUI interface, so that the pictures are provided for contrast segmentation in the next step; 4) picture processing: selecting the pictures, drawing a frame to select a region to be segmented, adjusting a segmentation threshold to acquire a relatively accurate segmentation result, and realizing visual interactive segmentation of the white spots in the spleen; and 5) quantitative analysis: realizing the quantitative analysis of the infection degree of the white spots in the spleen of the Pseudosciaena crocea by the proportion of the white spots in the spleen.

The invention discloses bacillus subtilis Q16 and application thereof, the strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20252232. According to the invention, the bacillus subtilis Q16 is separated from the body of the large yellow croaker and is added into the feed for the large yellow croaker, so that the growth performance, digestion, oxidation resistance and immunity of the large yellow croaker can be remarkably improved, and the intestinal flora composition structure of the large yellow croaker and the gene expression related to immunity and metabolism are changed.

The invention provides a method for culturing Pseudosciaena crocea fries of the Halitium family through multi-stage dynamic bait density regulation and control, and belongs to the technical field of aquaculture. The culture method comprises the following steps: feeding 5-10 rotifers per mL at the age of 0-6 days; feeding 3-5 copepoda nauplii / mL and 0.5-1 artemia newly hatched larvae / mL at the age of 7-15 days; feeding 2-4 artemia nauplii / mL and 10-40% of compound feed at the age of 16-25 days, and gradually increasing the input proportion of the compound feed along with the age of days; 10%-80% of artemia and 20%-90% of compound feed are fed at the age of 26-35 days, and the input proportion of the compound feed is gradually increased along with the age of days; feeding mixed feed for 36-45 days old; photosynthetic bacteria and bacillus subtilis are put into the water body in the whole culture process. Through precise feeding and dynamic regulation and control, the survival rate, growth uniformity and feeding efficiency of the fries of the Pseudosciaena crocea of the sal family are effectively improved, and the quality of the fries is remarkably optimized.

The application provides a whole-process cold-chain preservation method for fresh large yellow croaker, which is targeted to enzyme inhibition and gradient microenvironment regulation. The method provided by the application comprises the following steps: 1, soaking the freshly caught large yellow croaker in seawater containing endogenous protease inhibitor peptides of the large yellow croaker within 30 minutes after catching, and reducing the core temperature of the fish body to 3-4 DEG C; after the soaking is completed, the surface moisture of the fish body is blown dry with sterile wind; 2, packaging the large yellow croaker after the surface moisture of the fish body is blown dry, and storing the packaged large yellow croaker in an environment with a temperature of-0.5-1 DEG C and a relative humidity of 65%-75%, so as to realize the preservation of the fresh large yellow croaker. The method provided by the application can greatly improve the preservation effect, and the endogenous protease inhibitor peptides of the large yellow croaker do not destroy the nutritional components of the fish meat, do not change the original flavor, have no chemical preservative residues, meet the green food standard, and are safer than the existing chemical preservation and soaking preservation technologies.

This invention relates to equipment for processing large yellow croaker, and more particularly to a continuous production apparatus and method for marinating large yellow croaker. The continuous production apparatus for marinating large yellow croaker includes a frame, a controller, a horizontal conveying mechanism, and a shape detection mechanism, a punching mechanism, and a marinating mechanism arranged sequentially along the feeding direction of the horizontal conveying mechanism. The shape detection mechanism is located above the horizontal conveying mechanism and is used to detect the shape of the belly and back of the large yellow croaker respectively. The controller is used to receive information sent by the shape detection mechanism and generate a belly punching feed table and a back punching feed table. The two punching mechanisms punch holes in the belly and back of the fish according to the belly punching feed table and the back punching feed table respectively. The marinating mechanism is used to store and marinate the punched large yellow croaker. The continuous production apparatus for marinating large yellow croaker provided by this invention not only facilitates full marinating of the large yellow croaker and improves the marinating effect, but also meets the punching accuracy requirements for different fish sizes and shapes, avoiding damage to the fish meat.

The present application belongs to the technical field of fish genetic identification, discloses a large yellow croaker wild and domestication population typing identification method based on 10 universal SNPs sites, and specifically discloses a set of SNP molecular markers for identifying large yellow croaker wild and domestication samples. The present application provides a set of SNP molecular markers for identifying large yellow croaker wild and domestication samples. By detecting the SNP molecular markers, the identification of large yellow croaker wild and domestication samples can be realized only by using a multiplex PCR reaction system and SNaPshot typing sequencing. The operation on various genetic analysis instruments can realize automatic typing and identification of large yellow croaker wild samples and large yellow croaker domestication samples. Compared with the current method for identifying large yellow croaker populations, the method is faster, has higher detection flux, and more importantly, has very high accuracy and cumulative exclusion rate.

The application relates to the technical field of aquatic product monitoring, and discloses a large yellow croaker swimming speed monitoring method and system based on target detection, which comprises the following steps: acquiring a to-be-monitored image of a target water area, real-time environmental flow rate data and real-time water body turbidity data of the target water area; performing target detection on the to-be-monitored image to obtain coordinate data of the large yellow croaker; performing linkage verification on the coordinate data to obtain verified coordinate data; determining real-time posture features of the large yellow croaker according to the verified coordinate data; optimizing the real-time posture features through a flow rate phase joint mechanism to obtain optimized posture features; and constructing a posture fluid coupling model according to the optimized posture features and the real-time environmental flow rate data to determine the swimming speed of the large yellow croaker. The application fully considers the influence of the posture of the large yellow croaker and the environment, and combines the biomechanical features to realize accurate calculation of the real swimming speed of the large yellow croaker.

The invention belongs to the technical field of breeding, and particularly relates to a low-cost larimichthys crocea parent fish feed with high vegetable protein replacing fish meal. The invention discloses a feed additive, which accounts for the total weight of the feed. The low-cost pseudosciaena crocea parent fish feed comprises 10% of Peru steam fish meal, 23% of soybean protein concentrate, 12.5% of corn protein powder, 8.25% of shelled soybean meal, 18.4% of high gluten flour, 8% of chicken meal, 3% of blood cell powder, 2.5% of feather meal, 2% of soybean oil, 3.5% of fish oil, 2% of soybean lecithin oil, 1% of mineral premix, 0.2% of vitamin premix, 1.25% of beer yeast, 0.1% of vitamin C phosphate, 0.1% of glucan and 2% of monocalcium phosphate. The traditional Chinese medicine composition is prepared from, by weight, 0.6% of squid paste, 0.1% of ethoxyquin, 1% of taurine and 0.5% of choline chloride. The larimichthys crocea feed is reasonable in formula design, soybean protein concentrate and the like are used for replacing part of fish meal in traditional larimichthys crocea feed, the feed manufacturing cost and the larimichthys crocea parent fish feeding cost can be effectively saved, and meanwhile the growth performance of the larimichthys crocea parent fish is not remarkably affected.

The invention relates to a Pseudomonas plecoglossicida pldB gene deletion mutant strain and a preparation method and application thereof, and belongs to the technical field of microorganisms and the field of algae preservation, the pldB gene of the mutant strain is deleted or inactivated, the nucleotide sequence of the pldB gene is shown as SEQ ID NO: 1, and the GenBank accession number of the Pseudomonas plecoglossicida is CP031146.1. The virulence of the mutant strain is obviously reduced compared with that of a wild strain of pseudomonas plecoglossicida; the strain can induce appropriate immune response of a host and up-regulate expression of anti-inflammatory factors, is beneficial to relieving immune pathological injury, has good attenuated live vaccine potential, and provides a brand new vaccine candidate strain for prevention and control of the visceral white-spot disease of the large yellow croaker.

The invention provides a larimichthys crocea head kidney macrophage line as well as a construction method and application thereof, belongs to the technical field of cell engineering, and particularly provides the larimichthys crocea head kidney macrophage line which is named as LYC-hK and has the preservation number of CCTCC NO: C2025234. The larimichthys crocea head kidney macrophage line is constructed through the steps S1-S6, and the construction method of the larimichthys crocea head kidney macrophage line is applied to the field of cell engineering. The larimichthys crocea head kidney macrophage line provided by the invention can be used for research on pathogenic infection and pathogenic mechanism of larimichthys crocea, research on anti-infection immune response of larimichthys crocea and research on exogenous gene expression.

The utility model relates to the technical field of feeding equipment, and discloses large yellow croaker test feeding equipment which comprises a machine body and a belt, feeding ports are formed in the front end and the rear end of the right side of the top wall of the machine body, a feeding box is fixedly connected to the right side of the top wall of the machine body, and a box cover is arranged on the top wall of the feeding box; a first motor is arranged at the rear end of the right side of the outer wall of the machine body, a driving wheel is fixedly connected to the output end of the first motor, a first screw rod is fixedly connected to the left side of the outer wall of the driving wheel and rotationally connected to the rear side of the interior of the machine body, and a second screw rod is rotationally connected to the front side of the interior of the machine body. According to the feed conveying device, the first motor is started to drive the driving wheel and the first screw rod to rotate, meanwhile, the driving wheel drives the driven wheel and the second screw rod to rotate through the belt, feed conveying is achieved, the feed conveying amount can be more accurately controlled to a certain degree by conveying the feed through the screw rod, and therefore the feeding amount of all the discharging pipes is more uniform.

The invention relates to the technical field of fish abnormity identification, and discloses a large yellow croaker abnormal behavior identification method and system based on a radio frequency signal, and the method comprises the steps: obtaining the radio frequency signal of a marked fish, carrying out the feature extraction, obtaining a behavior feature index, and obtaining a first abnormity probability according to the behavior feature index and a health baseline; obtaining a behavior signal of a fish school, calculating a group behavior entropy, and obtaining a second abnormal probability according to the group behavior entropy and an entropy threshold; obtaining an initial abnormal fish school according to the first abnormal probability and the second abnormal probability; acquiring underwater acoustic signals and image data of the initial abnormal fish school, performing feature extraction on the underwater acoustic signals to obtain acoustic features, and performing feature statistics on the image data to obtain image features; and inputting the acoustic features and the image features into an abnormal recognition model, and carrying out abnormal behavior secondary recognition. According to the method, through cooperative judgment of the group behavior entropy and the behavior track and cross validation of multi-modal data fusion, accurate identification of the abnormal behaviors of the large yellow croakers is realized.

The application relates to a haplotype of a SNP site related to the fullness degree of Larimichthys crocea, a primer set and application thereof, and belongs to the technical field of molecular markers. Seven SNP sites related to the fullness degree of Larimichthys crocea are screened, and primers for amplifying the SNP sites are provided. The SNP sites or the primers can assist the breeding of new varieties with the fullness degree, can greatly shorten the breeding period, reduce the dependence on phenotypic traits, improve the selection and breeding efficiency, and have a good application prospect.

The invention provides a method for efficiently inducing larimichthys crocea triploid by using hydrostatic pressure, which comprises the following steps of: immediately transferring fertilized eggs into a hydrostatic pressure device 2 minutes and 30 seconds after artificial insemination of larimichthys crocea sperm eggs, applying pressure of 7000-8000 PSI, and performing hydrostatic pressure treatment for 3 minutes. Wherein in the hydrostatic pressure treatment process, it should be ensured that the pressure is stable, and the fluctuation range does not exceed + / -1%. According to the method, the time and pressure of hydrostatic pressure treatment are accurately controlled, the triploid induction rate can be 90% or above, and the induction rate is high. The damage to fertilized eggs is reduced through optimized processing parameters, the hatching rate of the fries can reach 70% or above, and the hatching rate is high. In addition, the hydrostatic pressure treatment time is only 3 minutes, the operation is efficient, the method is more suitable for large-scale production compared with other methods, and the triploid cultivation efficiency is improved.

The application belongs to the technical field of fish genetic identification, and discloses a multi-site identification method for wild large yellow croaker of Lutjanus Russellii based on SNaPshot technology, and specifically discloses a group of SNP molecular markers for identifying wild large yellow croaker of Lutjanus Russellii, wherein by detecting the SNP molecular markers, the identification of wild large yellow croaker of Lutjanus Russellii in wild large yellow croaker samples can be realized only by using a simple multiplex PCR reaction system and SNaPshot typing sequencing. The wild large yellow croaker samples of Lutjanus Russellii can be automatically typed and identified only by operating on various genetic analysis instruments. Compared with the method for identifying wild large yellow croaker of Lutjanus Russellii, the method is more accurate, faster and higher in detection flux, and has higher accuracy and cumulative exclusion rate.

The invention discloses a large yellow croaker living body transportation method based on multi-dimensional regulation and control, which comprises the following steps: (1) fasting temporary rearing: putting large yellow croakers into a temporary rearing pond, and stopping feeding 2-3 days before transportation; (2) performing bagging pretreatment: performing gradient cooling on the large yellow croaker culture water body to enable the fish body to enter a semi-dormant state, then filling the fish body into an oxygen filling bag, and adding montmorillonite-probiotic microspheres into the oxygen filling bag to cooperatively keep alive with an anti-stress agent; (3) boxing: oxygenating the oxygenating bag and then moving the oxygenating bag into a heat preservation transport case; and (4) transportation: controlling the water temperature in the transportation process to be 12 + / -0.5 DEG C and the transportation time to be less than or equal to 36 hours. According to the method, physical and chemical means are coupled, the survival rate of the large yellow croaker can be stably increased to 90% or above within 36 h during long-distance transportation, meanwhile, the pathogen infection risk after transportation is remarkably reduced, and a reliable technical path is provided for industrial loss reduction, efficiency improvement and standardized upgrading.

The invention provides an RPA (recombinase polymerase amplification) detection kit, an RPA-LFD (lateral flow dipstick) detection system and an RPA-LFD detection method. The sequences of an upstream primer and a downstream primer used in the detection are respectively shown as SEQ ID NO. 1 and SEQ ID NO. 2; the sequence of the probe is as shown in SEQ ID NO.3, and tetrahydrofuran is inserted into the probe. The invention further provides a trypanosome RPA (recombinase polymerase amplification) detection kit and a trypanosome RPA-LFD (lateral flow dipstick) detection system containing the detection kit, and also provides a corresponding detection method. The whole detection process can be completed within 23 minutes, the operation is simple and convenient, and the result is visible. Compared with an existing detection method, the method has the advantages of being rapid, sensitive, high in specificity, suitable for on-site operation and the like, and is suitable for early diagnosis and epidemiological investigation of the trypanosomiasis in the large yellow croaker culture process.

The invention discloses a method for identifying DNA (deoxyribonucleic acid) fragments of male parent nibea albiflora in a gynogenesis pseudosciaena crocea genome. According to the scheme, the pseudosciaena crocea genome is used as a reference coordinate system; male parent positive sites in a gynogenesis pseudosciaena crocea genome are screened by identifying the specific variation sites of the nibea albiflora and analyzing the genotypes of the gynogenesis pseudosciaena crocea at the specific variation sites of the nibea albiflora; performing multi-sequence comparison, calculating the number of positive sites in comparison blocks and the proportion, filtering candidate segments and the like to establish the method for identifying the genetic material from the male parent nibea albiflora in the gynogenetic large yellow croaker genome. At present, related identification is mainly carried out through comparative analysis and microsatellite marking; the methods have the problems of high cost, low positive rate and the like; according to the scheme, genetic materials derived from male parent nibea albiflora in a gynogenetic pseudosciaena crocea genome are comprehensively and accurately screened on the whole genome level, the economic character source of gynogenetic progeny can be defined, and application such as variety identity identification and variety right protection is facilitated by developing specific molecular markers.

The application discloses a large yellow croaker sea-land relay breeding management method and system based on segmented breeding optimization, which comprises the following steps: acquiring breeding environment and biological response data, carrying out breeding environment-biological coupling modeling, and obtaining a coupled digital twin; constructing a growth-quality collaborative prediction model by a physical information neural network to obtain growth-quality collaborative prediction information; establishing a multi-agent collaborative learning framework, combining the coupled digital twin for repeated trial-and-error training, and obtaining a multi-agent breeding strategy model; deploying the multi-agent breeding strategy model on an edge computing node, generating a breeding management optimization strategy according to the current breeding density and fish population state; when the large yellow croaker meets the pool-out standard, quality detection is carried out to generate a marketing scheme, and if the current time window is not the optimal marketing window, breeding progress planning management is carried out based on the breeding situation of the remaining breeding units. The application realizes intelligent management of the whole sea-land relay process and improves the breeding efficiency and economic benefits of the large yellow croaker.

The application relates to a haplotype of a low-temperature-resistant SNP site of Larimichthys crocea, a primer set and application thereof, and belongs to the technical field of molecular markers. Seven SNP sites significantly related to body length are identified through whole genome association analysis. Single base change in the sites can significantly affect the low-temperature resistance. The application also provides a primer set for amplifying the SNP sites. The advantage haplotype constructed based on the SNP sites can be used for molecular assisted breeding of a new strain of Larimichthys crocea with low-temperature resistance, significantly shortens the breeding cycle, improves the selection accuracy, and overcomes the limitations of traditional breeding on phenotype. The application provides an efficient molecular tool for Larimichthys crocea germplasm improvement, and has an important application prospect.

The application provides a domestication method of wild Pseudosciaena heterura fry, and belongs to the technical field of aquaculture. The application comprises the following steps: capturing wild Pseudosciaena heterura fry, transporting the wild Pseudosciaena heterura fry to a breeding pond for temporary breeding, and then sequentially performing live bait domestication, ice fresh bait domestication, soft granular feed domestication and artificial compound granular feed domestication breeding. The application gradually transitions the wild Pseudosciaena heterura fry to special artificial compound feed by feeding the live bait that is preferred by the wild Pseudosciaena heterura fry, so that the feeding habit and the digestive system of the wild Pseudosciaena heterura fry gradually adapt. The wild Pseudosciaena heterura fry can successfully switch to artificial compound feed after 20 days of domestication, the survival rate is more than 63%, and no batch death phenomenon occurs during the domestication. The application effectively improves the survival rate of the wild Pseudosciaena heterura fry domesticated into artificial breeding condition fry, and provides guarantee for solving the construction of the artificial breeding basic parent fish population.

The invention relates to the technical field of aquaculture, in particular to a land-sea relay breeding method for Pseudosciaena crocea of the sal family. Through the land-sea relay breeding mode of land-based factory fry breeding, land-sea transition domestication and deepwater net cage breeding, the defects of a single environment breeding mode can be effectively overcome, and the growth efficiency, the health level and the product quality of the Pseudosciaena crocea in all stages are improved; and the overall economic benefit and market competitiveness of the industry can be greatly improved, and the method has remarkable popularization and application value and wide development prospects.

The invention discloses a preparation method and application of a pseudosciaena crocea antibacterial peptide Elabela, a pseudosciaena crocea antibacterial peptide gene Elabela is obtained through gene cloning, and the nucleotide sequence of the pseudosciaena crocea antibacterial peptide gene Elabela is shown as SEQ ID NO.1. The antibacterial peptide Elabela is further prepared through prokaryotic expression and purification, and the amino acid sequence of the antibacterial peptide Elabela is as shown in SEQ ID NO. 2. The antibacterial peptide Elabela prepared by the invention has broad-spectrum antibacterial activity, and can be used for killing gram-negative bacteria and gram-positive bacteria. The antibacterial peptide prepared by the invention can be developed into anti-bacterial infection drugs or feed additives, and is applied to prevention and treatment of bacterial diseases of aquaculture animals.

The application provides a high-collagen trait-associated SNP marker combination of Pseudosciaena crocea and breeding application. Specifically provided is application of a chip loaded with the high-collagen trait-associated SNP marker combination of Pseudosciaena crocea in high-collagen trait genotyping or high-collagen trait breeding, and the SNP marker combination is composed of 12 SNP molecular markers, the location of each SNP molecular marker on a chromosome, an altered allele, and reference gene information are shown in Table 1. The SNP marker combination of the application can be used to determine the genetic potential of collagen content of individuals in early offspring, and the best individuals with high collagen potential of a certain scale can be selected in advance, and the selected individuals are bred and cultivated to parents, only the target individuals are bred and cultivated subsequently, and a large number of offspring seedlings do not need to be bred and cultivated in the breeding process, so that the breeding cost can be greatly reduced.

The invention relates to a Pseudosciaena crocea geographical population identification method combining otolith and morphological measurement. The method comprises the following steps: obtaining a Pseudosciaena crocea sample to be identified; obtaining morphological measurement data; performing first standardization processing on the morphological measurement data to obtain standardized morphological measurement data, inputting the standardized morphological measurement data into a pre-established morphological discrimination model, and outputting a first discrimination result through the morphological discrimination model; acquiring sagittal stone of the large yellow croaker sample to be identified; obtaining landmark point distance measurement data based on a preset otolith landmark point through sagittal otolith; performing second standardization processing on the landmark point distance measurement data to obtain standardized landmark point distance measurement data, inputting the standardized landmark point distance measurement data into a pre-established otolith discrimination model, and outputting a second discrimination result through the otolith discrimination model; and performing final identification based on the first judgment result and the second judgment result. The method can accurately, simply and conveniently identify three geographical populations of the Larimichthys crocea sal ammoniac family, the Fujian eastern Guangdong family and the Dai-qu family in batches.

The invention discloses a crossbreeding method for breeding pseudosciaena crocea in spring and summer and breeding pseudosciaena crocea in autumn. The crossbreeding method comprises the following steps: 1) screening high-quality parents of a spring and summer breeding group and an autumn breeding group; (2) adjusting the temperature and the illumination period, simultaneously carrying out nutrient enrichment, and carrying out reproduction regulation on the parent fish so as to synchronize gonad development of the parent fish; 3) performing artificial spawning induction by utilizing exogenous hormone, and performing natural hybrid fertilization or artificial dry-method fertilization according to a hybrid combination of breeding female fish in spring and summer, breeding male fish in autumn and breeding female fish in autumn and breeding male fish in spring and summer; 4, the obtained fertilized eggs are hatched and subjected to fry rearing, and finally the hybrid F1 new germplasm is obtained.The directional controllable hybridization of the two breeding populations is achieved, the genetic diversity of the large yellow croaker is increased, the germplasm degeneration problem is solved, and the filial generation has remarkable advantages in the aspects of growth speed, gonad development and the like.