144 results about "Larimichthys crocea" patented technology

The invention relates to the field of aquaculture, in particular to a preparation method and application of a larimichthys crocea iridovirus recombinant live vector vaccine. According to the invention, replication-deficient type 5 adenovirus is selected as a target gene expression vector, a recombinant adenovirus vector comprising main capsid protein of iridovirus is constructed, recombinant virus is obtained, and the safe and effective large yellow croaker iridovirus disease recombinant live vector vaccine is created. According to the vaccine, the larimichthys crocea is immunized in a soaking or bait feeding mode, the CPM of the larimichthys crocea immunized by the vaccine is 12.0%, and the CPM of the fish without being immunized by the vaccine is 62.0%. In addition, the RPS of the inoculated fish and the RPS of the empty carrier control group fish are 80.65% and 9.68% respectively. The method is at a leading level in the field of pseudosciaena crocea biomedicine at present, and creativity is remarkable.

The invention relates to haplotypes of cobia fatness degree related SNP sites, a primer group and application of the haplotypes and the primer group, and belongs to the technical field of molecular markers, seven SNP sites related to cobia fatness degree are screened, primers for amplifying the SNP sites are provided, breeding of new varieties with fatness degree characters can be assisted by utilizing the SNP sites or the primers, and breeding of cobia fatness degree characters is facilitated. The method can greatly shorten the breeding period, reduce the dependence on phenotypic characters, improve the selective breeding efficiency, and have good application prospects.

The invention provides an artificial breeding method of wild sal-family large yellow croaker, and belongs to the technical field of aquatic animal breeding. The invention provides an artificial breeding method of wild sal-family pseudosciaena crocea, which comprises the following steps: domesticating the wild sal-family pseudosciaena crocea, then carrying out intensive cultivation, and after the wild sal-family pseudosciaena crocea is cultivated until gonad is mature, carrying out artificial spawning induction and natural fertilization on the obtained parent fish of the sal-family pseudosciaena crocea to obtain fertilized eggs; the fertilized eggs are subjected to incubation and fry cultivation, and sal-family large yellow croaker fries are obtained; the artificial spawning method comprises the step of injecting hormone into parent fish in two steps. According to the method, favorable conditions are created for large-scale breeding of the sal-family pseudosciaena crocea, and a large number of artificially bred sal-family pseudosciaena crocea fry groups can be obtained by adopting the method.

The invention belongs to the technical field of biology, and particularly relates to application of hepcidin expression quantity in larimichthys crocea liver as larimichthys crocea visceral white-spot disease resistance evaluation index and a construction method. The hepcidin expression quantity in the larimichthys crocea liver is applied as the larimichthys crocea visceral white-spot disease resistance evaluation index. The construction method of the resistance evaluation index comprises the following steps: taking healthy small yellow croaker intraperitoneal injection pseudomonas proteinus strain bacterial liquid, and determining a half lethal dose in 96 hours; the method comprises the following steps: injecting a half lethal dose of bacterial liquid of pseudomonas proteinus strains into the abdominal cavity of healthy small yellow croaker for 96 hours, and after infection, taking different tissues to carry out hepcidin gene expression fluorescent quantitative PCR analysis; a regression model of gene expression quantity and bacterium loading quantity is established through fluorescence quantification, and the relationship between hepcidin expression quantity and disease resistance is constructed. Accurate disease-resistant phenotype information is provided for development of breeding of visceral white-spot disease-resistant improved varieties of the small yellow croakers, the accuracy of disease-resistant breeding is promoted, early detection of diseases is realized, and prevention and treatment of the diseases are effectively guided.

The invention relates to a composition for treating large yellow croaker pyramidoniasis as well as a preparation method and application thereof, and belongs to the technical field of aquaculture. The composition comprises the following components: curcumin, bile acid, beta glucan, bacillus subtilis freeze-dried powder, marine red yeast freeze-dried powder, vitamin C, vitamin E and ethylicin. When the feed additive is added into basal feed to feed large yellow croakers infected with the trypanosomiasis, the death rate of sick fishes can be effectively reduced, the polypide loading capacity is remarkably reduced, and pathological injuries of livers and spleens of the large yellow croakers are relieved. In addition, the composition can also improve the non-specific immunity of the fish body, improve the liver function, enhance the antioxidant capacity of the liver, and improve the lipase activity of the intestinal tract at the same time. Therefore, the composition can be used as a medicine or a feed additive for treating the large yellow croaker pyramidoniasis, has the advantages of small dosage and remarkable effect, and provides a new technical approach for solving the problem that the current large yellow croaker pyramidoniasis lacks an effective prevention and control means.

The invention discloses a large model-based large yellow croaker growth stage feeding amount evaluation method and system, and belongs to the field of aquaculture, and the method comprises the steps: collecting underwater images of a fish pond in a plurality of shooting directions at the same time, and recognizing and segmenting a fish body image corresponding to each entity fish in each underwater image; determining the distance between an entity fish corresponding to the fish body image and a camera for collecting the fish body image, and extracting fish body appearance features of the fish body image; randomly selecting a fish body image from each shooting direction to form a fish body image association combination, and screening a second fish body image association combination from all possible fish body image association combinations according to the distance and fish body appearance features; and constructing three-dimensional point cloud data of the entity fishes according to the second fish body image association combination, fitting three-dimensional body shape parameters of the entity fishes through the three-dimensional point cloud data, and determining the final feeding amount of the fish school according to the three-dimensional body shape parameters of the entity fishes. According to the invention, the problem of low accuracy of the real-time feeding amount of the fish school can be solved.

The invention belongs to the technical field of fish genetic identification, discloses a multi-site sal-family wild large yellow croaker identification method based on an SNaPshot technology, and particularly discloses a group of SNP (Single Nucleotide Polymorphism) molecular markers for screening a sal-family wild large yellow croaker group. The identification of the sal-family wild pseudosciaena crocea in the pseudosciaena crocea wild population sample can be realized only by a simple multiple PCR reaction system and SNaPshot typing sequencing. And automatic typing and identification of the wild large yellow croaker sample of the large sal ammoniac family can be realized only by operating on various genetic analysis instruments. Compared with the existing method for identifying the wild large yellow croaker in the sal family, the method has the advantages that the typing is more accurate, the operation is quicker, the detection flux is higher, and meanwhile, the accuracy rate and the cumulative exclusion rate are higher.

The invention discloses a large yellow croaker origin traceability discrimination method based on an element and FTIR fusion fingerprint spectrum. The method comprises the following steps: S1, sample treatment; s2, data acquisition: S2.1, mineral element data acquisition, and S2.2, FTIR spectrum acquisition; and S3, data analysis. Precise classification of production areas can be realized through principal component analysis (PCA), clustering analysis (CA) and linear discriminant analysis (LDA), the overall discriminant accuracy of back substitution inspection of the discriminant model reaches 100%, and the overall discriminant accuracy of cross inspection reaches 97.3%. The element and infrared spectrum fusion fingerprint technology is applied to the source tracing of the large yellow croaker producing area, and the application prospect is wide.

The invention discloses a method for regulating out-of-season breeding of large yellow croakers bred in autumn. The method is characterized in that spawning in spring and summer is achieved through a three-stage technical system of biological safety prevention and control, precise environment regulation and control and reproductive physiological intervention. The method specifically comprises the following steps: 1) detecting trypanosomes; 2) parent fish screening; (3) parent fish rearing; 4) artificial propagation; 5) artificial incubation; and 6) seedling cultivation. According to the method, the out-of-season spring and summer breeding technology breakthrough of breeding the large yellow croaker in autumn is successfully achieved through nutrient enrichment, accurate temperature parameter regulation and control, gradual illumination regulation and reasonable hormone treatment. According to the technology, an adjustable reproduction synchronization scheme is provided for cross breeding between a spring reproduction group and an autumn reproduction group, and the technology has important application value for germplasm innovation of the large yellow croaker.

The invention discloses a large yellow croaker feeding behavior monitoring method and system based on target detection, and the method comprises the steps: carrying out the feature extraction of a large yellow croaker feeding video collected in real time, obtaining a visual behavior feature, and carrying out the feature extraction of a large yellow croaker acoustic signal collected in real time, and obtaining an acoustic behavior feature; performing time alignment on the visual behavior features and the acoustic behavior features through a preset cross-modal phase synchronization network to obtain a first feature pair, and performing semantic alignment on the first feature pair through a preset attention mechanism to obtain a second feature pair; performing feature fusion on the second feature pair to obtain a multi-modal feature, and outputting an ingestion behavior recognition result according to the multi-modal feature through a preset first ingestion behavior recognition model; and when the ingestion behavior result belongs to the abnormal ingestion behavior category, performing ingestion behavior abnormal early warning according to a preset grading early warning mechanism. The accuracy of large yellow croaker feeding behavior monitoring can be improved.

This invention discloses a device and method for the propagation, release, and rewilding training of large yellow croaker fry, relating to the field of marine biological resource conservation technology. The device includes: a water tank containing water, with multiple artificial reefs placed inside; a light source positioned above the water tank; multiple wave-making pumps evenly distributed along one side of the inner wall of the water tank; a release structure for releasing predator pheromones into the water tank; a camera mounted on the inner wall of the water tank; and a controller electrically connected to the camera, light source, and wave-making pumps. The camera collects behavioral characteristic information of the large yellow croaker during rewilding training and sends this information to the controller. A fish behavior analysis system comprehensively analyzes the swimming and reef usage of the large yellow croaker fry in the water tank to determine the effectiveness of the rewilding training. The advantages of this invention are enhanced ability of large yellow croaker fry to adapt to the marine environment and improved survival rate of released fry.

This invention discloses a multi-unit composite stress tiered breeding device and method for juvenile large yellow croaker, belonging to the field of aquaculture breeding technology. The device uses a central control unit as its sole core, forming a fully integrated unidirectional closed-loop drive system. Four independent breeding units are connected through a transparent channel with a centrally located aeration head, forming a physical flow-limiting isolation structure. Each unit independently regulates water temperature, salinity, and dissolved oxygen, creating a multi-factor composite stress gradient. The breeding method employs a three-factor linear equal-gradient synergistic progressive setting, utilizing the juvenile fish's autonomous adaptation and avoidance instincts to achieve cross-unit migration and natural selection. Combined with contour recognition and dynamic tracking algorithms, it monitors the fish population status and mortality rate in real time, and identifies superior offspring with comprehensive stress resistance through secondary verification under extreme stress. This invention offers high screening accuracy and automation, increasing the survival rate of superior individuals by over 40%, reducing labor costs by over 60%, and allowing for flexible adjustment of device parameters. It can be extended to the stress resistance breeding of other marine economic fish species.

The application discloses a method for quantitatively analyzing the infection degree of white spots in the spleen of Pseudosciaena crocea based on machine learning, and relates to a threshold segmentation technology of machine learning. 1) Data acquisition: dissect the Pseudosciaena crocea infected with internal visceral white spot disease, use a high-definition camera to shoot the spleen, and acquire pictures of the white spots in the spleen to be quantitatively analyzed; 2) creation of an interactive segmentation GUI interface: used for reading the pictures of the white spots in the spleen of the Pseudosciaena crocea and automatically converting the pictures into binary images; 3) running of a main program: the main program is written by a python code and is used for the GUI interface to display the selected pictures on the interactive segmentation GUI interface, so that the pictures are provided for contrast segmentation in the next step; 4) picture processing: selecting the pictures, drawing a frame to select a region to be segmented, adjusting a segmentation threshold to acquire a relatively accurate segmentation result, and realizing visual interactive segmentation of the white spots in the spleen; and 5) quantitative analysis: realizing the quantitative analysis of the infection degree of the white spots in the spleen of the Pseudosciaena crocea by the proportion of the white spots in the spleen.

The invention discloses bacillus subtilis Q16 and application thereof, the strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20252232. According to the invention, the bacillus subtilis Q16 is separated from the body of the large yellow croaker and is added into the feed for the large yellow croaker, so that the growth performance, digestion, oxidation resistance and immunity of the large yellow croaker can be remarkably improved, and the intestinal flora composition structure of the large yellow croaker and the gene expression related to immunity and metabolism are changed.

The invention provides a method for culturing Pseudosciaena crocea fries of the Halitium family through multi-stage dynamic bait density regulation and control, and belongs to the technical field of aquaculture. The culture method comprises the following steps: feeding 5-10 rotifers per mL at the age of 0-6 days; feeding 3-5 copepoda nauplii / mL and 0.5-1 artemia newly hatched larvae / mL at the age of 7-15 days; feeding 2-4 artemia nauplii / mL and 10-40% of compound feed at the age of 16-25 days, and gradually increasing the input proportion of the compound feed along with the age of days; 10%-80% of artemia and 20%-90% of compound feed are fed at the age of 26-35 days, and the input proportion of the compound feed is gradually increased along with the age of days; feeding mixed feed for 36-45 days old; photosynthetic bacteria and bacillus subtilis are put into the water body in the whole culture process. Through precise feeding and dynamic regulation and control, the survival rate, growth uniformity and feeding efficiency of the fries of the Pseudosciaena crocea of the sal family are effectively improved, and the quality of the fries is remarkably optimized.

The application provides a whole-process cold-chain preservation method for fresh large yellow croaker, which is targeted to enzyme inhibition and gradient microenvironment regulation. The method provided by the application comprises the following steps: 1, soaking the freshly caught large yellow croaker in seawater containing endogenous protease inhibitor peptides of the large yellow croaker within 30 minutes after catching, and reducing the core temperature of the fish body to 3-4 DEG C; after the soaking is completed, the surface moisture of the fish body is blown dry with sterile wind; 2, packaging the large yellow croaker after the surface moisture of the fish body is blown dry, and storing the packaged large yellow croaker in an environment with a temperature of-0.5-1 DEG C and a relative humidity of 65%-75%, so as to realize the preservation of the fresh large yellow croaker. The method provided by the application can greatly improve the preservation effect, and the endogenous protease inhibitor peptides of the large yellow croaker do not destroy the nutritional components of the fish meat, do not change the original flavor, have no chemical preservative residues, meet the green food standard, and are safer than the existing chemical preservation and soaking preservation technologies.

The present invention discloses a yellow-colored large yellow croaker and a breeding method thereof, comprising the following steps: (1) simultaneously constructing group breeding and family breeding, selecting wild large yellow croaker caught at sea as a female parent according to the blue-yellow value of the dorsal and abdominal regions; selecting large yellow croaker from a cultured group as a male parent according to the blue-yellow value of the dorsal and abdominal regions; selecting wild large yellow croaker caught at sea as female and male parents of a family according to the blue-yellow value of the dorsal and abdominal regions; (2) selecting F2 parents from group breeding and yellow-colored family breeding; (3) selecting F3 parents from yellow-colored large yellow croaker; (4) selecting F4 parents from yellow-colored large yellow croaker; and (5) selecting Fn-generation parents from yellow-colored large yellow croaker, where n is a positive integer ≥5. By adopting this method and breeding for more than five generations, a new variety with a stable golden body surface color can be formed.

The application provides a method for on-site rapid artificial insemination of wild Pseudosciaena crocea of Lutjanidae, and belongs to the technical field of aquatic animal breeding. Wild parent fish of Pseudosciaena crocea of Lutjanidae are captured in the breeding season; sperm is collected from the selected sexually mature male parent fish on the capture site; after artificial sperm collection, sexually mature female parent fish are selected, and fish eggs are collected; high-quality fertilized eggs are obtained through artificial insemination; the artificial insemination comprises the following steps: adding sperm into fish eggs, adding natural seawater for stirring, and finally washing with natural seawater. Through comprehensive regulation of key links in the whole process of artificial insemination, the purpose of obtaining high-quality fertilized eggs by using wild parent fish of Pseudosciaena crocea of Lutjanidae and carrying out artificial fry breeding is achieved.

This invention relates to equipment for processing large yellow croaker, and more particularly to a continuous production apparatus and method for marinating large yellow croaker. The continuous production apparatus for marinating large yellow croaker includes a frame, a controller, a horizontal conveying mechanism, and a shape detection mechanism, a punching mechanism, and a marinating mechanism arranged sequentially along the feeding direction of the horizontal conveying mechanism. The shape detection mechanism is located above the horizontal conveying mechanism and is used to detect the shape of the belly and back of the large yellow croaker respectively. The controller is used to receive information sent by the shape detection mechanism and generate a belly punching feed table and a back punching feed table. The two punching mechanisms punch holes in the belly and back of the fish according to the belly punching feed table and the back punching feed table respectively. The marinating mechanism is used to store and marinate the punched large yellow croaker. The continuous production apparatus for marinating large yellow croaker provided by this invention not only facilitates full marinating of the large yellow croaker and improves the marinating effect, but also meets the punching accuracy requirements for different fish sizes and shapes, avoiding damage to the fish meat.

The present application belongs to the technical field of fish genetic identification, discloses a large yellow croaker wild and domestication population typing identification method based on 10 universal SNPs sites, and specifically discloses a set of SNP molecular markers for identifying large yellow croaker wild and domestication samples. The present application provides a set of SNP molecular markers for identifying large yellow croaker wild and domestication samples. By detecting the SNP molecular markers, the identification of large yellow croaker wild and domestication samples can be realized only by using a multiplex PCR reaction system and SNaPshot typing sequencing. The operation on various genetic analysis instruments can realize automatic typing and identification of large yellow croaker wild samples and large yellow croaker domestication samples. Compared with the current method for identifying large yellow croaker populations, the method is faster, has higher detection flux, and more importantly, has very high accuracy and cumulative exclusion rate.

The application relates to the technical field of aquatic product monitoring, and discloses a large yellow croaker swimming speed monitoring method and system based on target detection, which comprises the following steps: acquiring a to-be-monitored image of a target water area, real-time environmental flow rate data and real-time water body turbidity data of the target water area; performing target detection on the to-be-monitored image to obtain coordinate data of the large yellow croaker; performing linkage verification on the coordinate data to obtain verified coordinate data; determining real-time posture features of the large yellow croaker according to the verified coordinate data; optimizing the real-time posture features through a flow rate phase joint mechanism to obtain optimized posture features; and constructing a posture fluid coupling model according to the optimized posture features and the real-time environmental flow rate data to determine the swimming speed of the large yellow croaker. The application fully considers the influence of the posture of the large yellow croaker and the environment, and combines the biomechanical features to realize accurate calculation of the real swimming speed of the large yellow croaker.

The invention belongs to the technical field of breeding, and particularly relates to a low-cost larimichthys crocea parent fish feed with high vegetable protein replacing fish meal. The invention discloses a feed additive, which accounts for the total weight of the feed. The low-cost pseudosciaena crocea parent fish feed comprises 10% of Peru steam fish meal, 23% of soybean protein concentrate, 12.5% of corn protein powder, 8.25% of shelled soybean meal, 18.4% of high gluten flour, 8% of chicken meal, 3% of blood cell powder, 2.5% of feather meal, 2% of soybean oil, 3.5% of fish oil, 2% of soybean lecithin oil, 1% of mineral premix, 0.2% of vitamin premix, 1.25% of beer yeast, 0.1% of vitamin C phosphate, 0.1% of glucan and 2% of monocalcium phosphate. The traditional Chinese medicine composition is prepared from, by weight, 0.6% of squid paste, 0.1% of ethoxyquin, 1% of taurine and 0.5% of choline chloride. The larimichthys crocea feed is reasonable in formula design, soybean protein concentrate and the like are used for replacing part of fish meal in traditional larimichthys crocea feed, the feed manufacturing cost and the larimichthys crocea parent fish feeding cost can be effectively saved, and meanwhile the growth performance of the larimichthys crocea parent fish is not remarkably affected.

The invention discloses large yellow croaker low-stress mechanical catching and running water transportation integrated equipment, and belongs to the technical field of fishery equipment.The large yellow croaker low-stress mechanical catching and running water transportation integrated equipment comprises a floating body, an opening is formed in the middle of the floating body, a netting used for containing cultured organisms is arranged at the bottom of the floating body, a supporting frame is arranged at the bottom of the floating body, and the supporting frame is arranged on the inner side of the netting and is adjacent to the netting; the supporting frame is composed of metal rods which are arranged in a staggered mode, a spacing distance exists between the metal rods, the problems of high stress and low efficiency easily caused by a traditional net depending on which large yellow croakers are caught are solved, and integrated low-stress operation of a catching device and a transporting device is achieved through the scheme.

The invention relates to a Pseudomonas plecoglossicida pldB gene deletion mutant strain and a preparation method and application thereof, and belongs to the technical field of microorganisms and the field of algae preservation, the pldB gene of the mutant strain is deleted or inactivated, the nucleotide sequence of the pldB gene is shown as SEQ ID NO: 1, and the GenBank accession number of the Pseudomonas plecoglossicida is CP031146.1. The virulence of the mutant strain is obviously reduced compared with that of a wild strain of pseudomonas plecoglossicida; the strain can induce appropriate immune response of a host and up-regulate expression of anti-inflammatory factors, is beneficial to relieving immune pathological injury, has good attenuated live vaccine potential, and provides a brand new vaccine candidate strain for prevention and control of the visceral white-spot disease of the large yellow croaker.

The invention provides a larimichthys crocea head kidney macrophage line as well as a construction method and application thereof, belongs to the technical field of cell engineering, and particularly provides the larimichthys crocea head kidney macrophage line which is named as LYC-hK and has the preservation number of CCTCC NO: C2025234. The larimichthys crocea head kidney macrophage line is constructed through the steps S1-S6, and the construction method of the larimichthys crocea head kidney macrophage line is applied to the field of cell engineering. The larimichthys crocea head kidney macrophage line provided by the invention can be used for research on pathogenic infection and pathogenic mechanism of larimichthys crocea, research on anti-infection immune response of larimichthys crocea and research on exogenous gene expression.

The utility model relates to the technical field of feeding equipment, and discloses large yellow croaker test feeding equipment which comprises a machine body and a belt, feeding ports are formed in the front end and the rear end of the right side of the top wall of the machine body, a feeding box is fixedly connected to the right side of the top wall of the machine body, and a box cover is arranged on the top wall of the feeding box; a first motor is arranged at the rear end of the right side of the outer wall of the machine body, a driving wheel is fixedly connected to the output end of the first motor, a first screw rod is fixedly connected to the left side of the outer wall of the driving wheel and rotationally connected to the rear side of the interior of the machine body, and a second screw rod is rotationally connected to the front side of the interior of the machine body. According to the feed conveying device, the first motor is started to drive the driving wheel and the first screw rod to rotate, meanwhile, the driving wheel drives the driven wheel and the second screw rod to rotate through the belt, feed conveying is achieved, the feed conveying amount can be more accurately controlled to a certain degree by conveying the feed through the screw rod, and therefore the feeding amount of all the discharging pipes is more uniform.

The invention relates to the technical field of fish abnormity identification, and discloses a large yellow croaker abnormal behavior identification method and system based on a radio frequency signal, and the method comprises the steps: obtaining the radio frequency signal of a marked fish, carrying out the feature extraction, obtaining a behavior feature index, and obtaining a first abnormity probability according to the behavior feature index and a health baseline; obtaining a behavior signal of a fish school, calculating a group behavior entropy, and obtaining a second abnormal probability according to the group behavior entropy and an entropy threshold; obtaining an initial abnormal fish school according to the first abnormal probability and the second abnormal probability; acquiring underwater acoustic signals and image data of the initial abnormal fish school, performing feature extraction on the underwater acoustic signals to obtain acoustic features, and performing feature statistics on the image data to obtain image features; and inputting the acoustic features and the image features into an abnormal recognition model, and carrying out abnormal behavior secondary recognition. According to the method, through cooperative judgment of the group behavior entropy and the behavior track and cross validation of multi-modal data fusion, accurate identification of the abnormal behaviors of the large yellow croakers is realized.

The application relates to a haplotype of a SNP site related to the fullness degree of Larimichthys crocea, a primer set and application thereof, and belongs to the technical field of molecular markers. Seven SNP sites related to the fullness degree of Larimichthys crocea are screened, and primers for amplifying the SNP sites are provided. The SNP sites or the primers can assist the breeding of new varieties with the fullness degree, can greatly shorten the breeding period, reduce the dependence on phenotypic traits, improve the selection and breeding efficiency, and have a good application prospect.

The invention provides a method for efficiently inducing larimichthys crocea triploid by using hydrostatic pressure, which comprises the following steps of: immediately transferring fertilized eggs into a hydrostatic pressure device 2 minutes and 30 seconds after artificial insemination of larimichthys crocea sperm eggs, applying pressure of 7000-8000 PSI, and performing hydrostatic pressure treatment for 3 minutes. Wherein in the hydrostatic pressure treatment process, it should be ensured that the pressure is stable, and the fluctuation range does not exceed + / -1%. According to the method, the time and pressure of hydrostatic pressure treatment are accurately controlled, the triploid induction rate can be 90% or above, and the induction rate is high. The damage to fertilized eggs is reduced through optimized processing parameters, the hatching rate of the fries can reach 70% or above, and the hatching rate is high. In addition, the hydrostatic pressure treatment time is only 3 minutes, the operation is efficient, the method is more suitable for large-scale production compared with other methods, and the triploid cultivation efficiency is improved.

The application belongs to the technical field of fish genetic identification, and discloses a multi-site identification method for wild large yellow croaker of Lutjanus Russellii based on SNaPshot technology, and specifically discloses a group of SNP molecular markers for identifying wild large yellow croaker of Lutjanus Russellii, wherein by detecting the SNP molecular markers, the identification of wild large yellow croaker of Lutjanus Russellii in wild large yellow croaker samples can be realized only by using a simple multiplex PCR reaction system and SNaPshot typing sequencing. The wild large yellow croaker samples of Lutjanus Russellii can be automatically typed and identified only by operating on various genetic analysis instruments. Compared with the method for identifying wild large yellow croaker of Lutjanus Russellii, the method is more accurate, faster and higher in detection flux, and has higher accuracy and cumulative exclusion rate.