194 results about "Larimichthys crocea" patented technology

The invention discloses a larimichthys crocea liver cell line and an establishment method thereof. The larimichthys crocea liver cell line is preserved in the China center for type culture collection (CCTCC) On October 31, 2013 and has a preservation number of CCTCC NO: C2013157. The larimichthys crocea liver cell line can realize serial passage, can provide a large amount of larimichthys crocea liver cell lines, can be used for pathogenic characteristic research, vaccine development, functional gene research and breeding research, has excellent properties, comprises fibroblast-like cells and mainly comprises cells having parapodiums stretching out and irregular shapes. The larimichthys crocea liver cell line has the advantages of exuberant division, short passage time, easy digestion, good adherence rate and hunger resistance. The establishment method has good repeatability. The larimichthys crocea liver cell line has good stability.

The invention relates to the technical field of aquaculture feedstuff, in particular to feedstuff additive premix material. The feedstuff additive comprises the following components according to the following weight portion: 150 to 450 portions of astaxanthin, 300 to 700 portions of dunaliella sallina, 150 to 400 portions of vitamin E, 300 to 600 portions of Chinese thorowax, 100 to 250 portions of canthaxanthin, 300 to 600 portions of antioxidant, and 150 to 1,000 portions of corn protein powder. The feedstuff additive has no innocuous and side residue, no drug resistance, environmental protection, and no adverse effect on the feedstuff palatability. The feedstuff additive can obviously improve the body color of fishes in the yellow and red color systems such as golden pompano, pseudobagrus fulvidraco, clarias fuscus, sea bream, large yellow croaker, and the like, can make the body color display natural yellow or red color, and can effectively improve the immunity, reproductive capacity and meat quality of cultivated fish. In addition, the feedstuff additive has stable raw material sources and a simple manufacturing process.

The invention discloses a graded culture method for large yellow croaker fry, which comprises the following steps of: performing fertilized egg incubation and membrane broken juvenile fish culture in an indoor nursery pond; when 20 percent of juvenile fish is cultured to reach the body length of 20 millimeters, training the juvenile fish by using a proper amount of eel powder and feed in the morning; when 50 percent of juvenile fish is cultured to reach the body length of 20 millimeters, performing reinforced training for 3 days by using full eel powder; transferring the fry with body length of not less than 20 millimeters to an outdoor secondary net cage for culture, wherein the culture density of the secondary net cage is 90 to 110 thousand pieces; transferring the fry with body length of not less than 30 millimeters to an outdoor tertiary net cage for culture, wherein the culture density of the tertiary net cage is 50 to 70 thousand pieces; transferring the fry with body length of not less than 40 millimeters to an outdoor quaternary net cage for culture, wherein the culture density of the quaternary net cage is 30 to 40 thousand pieces; and when 95 percent of fry is cultured to the body length of not less than 50 millimeters, obtaining the large yellow croaker fry capable of being put to breed. The graded culture method for the large yellow croaker fry has regular fry specification, high fry growth speed, high survival rate and good adaptability of the fry to the culture environment.

The invention provides a method for producing instant large yellow croaker roes, which comprises the steps of raw material selection-removal of fishy smell-stewing-grinding and sieving-frying-antioxidation processing-forming-drying-cooling-packing-finishing. The method is simple in process and low in production cost; and for the produced instant croaker roes, the fishy smell is removed, and the nutrition and the unique mouthfeeling of the roes are remained, therefore, the instant large yellow croaker roe is a new generation of low-heat healthy leisure food.

The invention relates to an artificial mixed breeding method for spotted maigre and large yellow croaker in marine aquaculture industry, and belongs to the technical field of marine culture. The breeding method includes the following steps, firstly pond-separated breeding maturation accelerating and parturition hastening of parent fish; secondly, same-pond breeding of spotted maigre and large yellow croaker fry; and thirdly, fry separating that's carried out through siphonage after the fry grows to be 2 to 3 cm in length. The pond-separated breeding maturation accelerating and parturition hastening of parent fish includes the maturation accelerating of the spotted maigre and the maturation accelerating of the large yellow croaker, and both the maturation accelerating of the spotted maigreand the maturation accelerating of the large yellow croaker include parent fish breeding and gonad maturation accelerating and parturition hastening. The same-pond breeding of spotted maigre and large yellow croaker fry includes same-pond hatching of the fertilized eggs of the spotted maigre and the large yellow croaker, prelarva prior period growing management, prelarva later perior growing management, postlarva period growing management and water quality regulation and control. 1.5 kg of fertilized eggs of the spotted maigre and the large yellow croaker are thrown into a mud pond of 32m<2>,and 696, 7000 juvenile fishes of 2 to 3 mm are bred at last, and the breeding rate per unit area is 2300/m<2>, as a result, the utilization rate of the breeding pond is greatly improved and the breeding cost is reduced.

The invention discloses a larimichthys crocea genetic sex related SNP (Single Nucleotide Polymorphism) marker and a primer and the application. The larimichthys crocea genetic sex related SNP marker is the sequence shown as in SEQ ID NO:1, and the 201th basic group from the 5' end is T or C. The individual is female larimichthys crocea when the SNP marker is CC genotype, and the individual is malelarimichthys crocea when the SNP marker is CT genotype. By identifying the molecular marker, the SNP marker has the characteristic of rapidly, conveniently, accurately identifying the genetic sex oflarimichthys crocea, moreover, the larimichthys crocea genetic sex related SNP marker also lays foundations for successfully carrying out the work of sex control and breeding of larimichthys crocea, developing monosex cultivation, developing selective breeding of the genome of the larimichthys crocea, and studying the sex determination molecule mechanism.

The invention mainly relates to Larimichthys crocea connetin source ACE inhibitory peptide sequences and applications thereof. The two kinds of peptide sequences respectively are WAR and WQR, and establish a basis for prevention and treatment of hypertension. Different on-line databases are used, and after multiple times of screening, the inhibitory peptides with high efficient ACE inhibition activity are obtained. WAR and WQR are artificially synthesized, in order to verify external ACE inhibition activity of the peptides by using a high performance liquid chromatography method. The inventionrelates to two kinds of active peptides with good external ACE inhibition activity. The two kinds of peptide sequences are used for developing health food with blood pressure adjusting effects, and the purpose for prevention, control, alleviation and auxiliary treatment of hypertension is realized by long-term usage.

A method for predicting cryptocaryoniosis in Larimichthys crocea relates to Larimichthys crocea, and belongs to a method of accurately predicting a water environmental factor at a certain future point-in-time to achieve advanced prediction on cryptocaryoniosis in Larimichthys crocea. The method includes: plugging a predicted value of the obtained water environmental factor in a mathematical discriminatory model for cryptocaryoniosis based on the water environmental factor; calculating a predicted value of the cryptocaryoniosis to generate the advanced prediction on the cryptocaryoniosis in Larimichthys crocea; and obtaining advanced prediction on the cryptocaryoniosis in Larimichthys crocea. The method is simple and convenient and capable of reflecting anural variation, parameters of the water environmental factor can be obtained according to a series of fitting equations only by providing month, the parameters can be calculated with a disease discriminatory model to finally obtain the predicted value of the cryptocaryoniosis, and accordingly, the method is quite convenient in use. For the specific cryptocaryoniosis, the method is convenient for farmers to timely take measures to control the disease and prevent and alleviate disasters.

The invention discloses a larimichthys crocea genome-wide SNP and InDel molecular marker method based on double enzyme digestion. The method comprises the following steps: 1) joint sequence design; 2) genome DNA enzyme digestion; 3) joint sequence connection; 4) sample mixing and PCR amplification; 5) high-throughput sequencing; 6) SNP locus excavation and analysis through sequencing data. According to the method, the modern molecular biology and the advanced high-throughput sequencing technology are combined, the method of combination of EcoRII and NlaIII double enzyme digestion is used, the genome-wide DNA molecules are subjected to enzyme digestion, DNA segments with certain lengths are obtained for carrying out library establishment sequencing and SNP molecular marker excavation, and the uniformly distributed genome-wide SNP locus information is obtained; the workload and cost of genome-wide marker analysis are greatly reduced, and the method is also applicable to the genome-wide marker analysis of other species; the SNP marker excavated through the method is applicable to the study fields such as animal and plant species identification, species genetic genealogy analysis, germplasm resource genetic diversity analysis and genetic breeding.

The invention discloses an immunopotentiator for large yellow croaker adults, which are prepared by uniformly mixing raw materials including fructooligosaccharides, mannan oligosaccharides, polysaccharide sulfates, yeast extract, vitamin C-2-phosphate and vitamin E-acetate. When added in feed in an amount of only 0.1 to 0.2 percent, the immunopotentiator can obviously improve the immunity and disease resistance in the large yellow croaker adults, so the survival rate of cultured large yellow croakers is improved by over 8 percent, and the immunoprotection rate is over 50 percent; and the immunopotentiator is free from toxic and side effects, residue and medicament resistance and avoids influencing the palatability of the feed. Therefore, with a small added amount, the immunopotentiator can obviously improve the immunity and disease resistance in the large yellow croaker adults, so that the culture survival rate is improved by over 8 percent and the immunoprotection rate is over 50 percent.

The invention discloses a device specially used for sonar-detecting whether large yellow croakers escape from a deepwater net-cage, comprising a computer, a probe which is arranged closely to the net-cage, and a digital to analog adaptive device which is connected with the computer; wherein, the probe is arranged at the external side of the net-cage; the probe comprises a sonar transducer and a flowmeter probe; the digital to analog adaptive device is connected with the probe and can convert the analog signal of the probe into the digital signal and send the signal to the computer; the computer receives the digital signal of the digital to analog adaptive device, processes the digital signal and judges whether the fish escapes or not according to the coupling result of the echo signal scanned by the flowing speed and sonar. The invention also discloses a sonar detection method which utilizes the device to detect whether the fish escapes from the deepwater net-cage simultaneously. The device and the method has the advantages that the device is not affected by water quality, the detection distance is far, the result is exact, the target objects can be intuitively and effectively monitored and detected, and the like.

The invention provides feeds for young larimichthys crocea. The feeds consist of the following air-dried components in parts by mass: 32 to 36 parts of fish meal, 2 to 3 parts of fish protein hydrolysate powder, 3 to 6 parts of spray-dried blood cell protein powder, 3 to 6 parts of shrimp meal, 3 to 5 parts of shrimp shell meal, 4 to 8 parts of krill meal, 7 to 10 parts of silkworm chrysalis meal, 4 to 5 parts of fermented soybean meal, 1 to 1.5 parts of sesame seed meal, 2 to 3 parts of corn protein powder, 10 to 12 parts of wheat flour, 3 to 4 parts of modified cassava starch, 1 to 2 parts of kelp powder, 1.5 to 2 parts of fish oil, 1.5 to 2 parts of soybean oil, 1.5 to 2 parts of soybean phospholipid oil, 1.5 to 2 parts of calcium dihydrogen phosphate, 1 part of traditional Chinese herbal medicinal compound preparation and 2 parts of a premix additive, wherein the traditional Chinese herbal medicinal compound preparation consists of the following substances in parts by weight: 12 parts of honeysuckles, 8 parts of tuber onion seeds, 10 parts of poria cocos, 3 parts of polygala tenuifolia, 4 parts of semen cuscutae, 3 parts of safflowers and 60 parts of rice hull powder. According to the feeds, the immunity of the young larimichthys crocea can be enhanced, visceral sarcoidosis can be prevented, better treatment effects on the young larimichthys crocea suffering from visceral sarcoidosis are achieved, and the survival rate of the larimichthys crocea cultivation is increased.

The invention relates to a method for cultivating large yellow croaker fingerling and a screening molecular marker applied to the method. The method comprises the following steps: 1) screening the related genes of growth axis of the large yellow croaker and the chain SSR thereof; 2) compounding a primer; 3) detecting SSR primer extension polymorphism; 4) marking and screening the growth related SSR; 5) marking and screening parent fish of large yellow croaker; 6) cultivating the large yellow croaker fingerling. The method can overcome the defects being short of rapid screening and cultivation for the large yellow croaker fingerling of the prior art and has the advantages of high speed, convenience, effectiveness and capability of accelerating the cultivating process of large yellow croaker.

The invention discloses a larimichthys crocea parent fish compound feed which is composed of the following raw material components in parts by weight: 1000 parts of brown fishmeal, 600-800 parts of white fish meal, 150-200 parts of puffed soybean meal, 60-80 parts of green gram starch, 150-200 parts of beer yeast powder, 30-50 parts of phaffia rhodozyma powder, 140-150 parts of liver powder, 80-100 parts of squid meal, 500-600 parts of high gluten flour, 100-200 parts of kelp powder, 40-60 parts of soya bean lecithin powder, 40-60 parts of fish oil, 10-15 parts of choline, 5-15 parts of taurine, 3-9 parts of VC, 1-3 parts of VE, 0.5-1 part of inositol, 20-40 parts of bone meal, 5-10 parts of monocalcium phosphate, 20-40 parts of salt, 10-20 parts of vitamin premix and 20-30 parts of mineral substance premix. According to the invention, the egg laying amount, spawning rate, fertility rate and hatching rate of larimichthys crocea parent fish and the survival rate of larimichthys crocea fries can be obviously improved, and the abnormality rate of the fries is reduced.

The invention discloses a feed for improving the quality of large yellow croakers, and a feeding method of the feed. The feed for improving the quality of the large yellow croakers comprises import fishmeal, fresh fish pulp, shrimp meal, scallop powder, fermented soybean meal, peeled soybean meal, yeast protein, soybean lecithin, calcium dihydrogen phosphate, high gluten flour, multivitamin multi-ore, a quality improver and fish oil, wherein the quality improver comprises two or three selected from a sea algae extract, cysteine hydrochloride and collagen small peptide in a proportion. The invention also discloses the feeding method of the feed. After the feed is used, the content of fat in the muscles of the large yellow croakers can be reduced, so that the lean meat percentage is increased; the contents of delicious amino acid and the like are increased, and especially, the contents of glutamic acid, leucine and aspartic acid are most remarkably increased. After the feed is used, the composition and the content of fatty acid of the large yellow croakers can be increased, and the contents of unsaturated fatty acid as well as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) which have important physiological functions are increased, so that the large yellow croakers are relatively good in mouth feel and nutrition and are relatively suitable for the requirements of consumers. Furthermore, the quality improver of the feed is a food or feed additive with a natural source, and thus the advantages of being poisonless, harmless, free of residue in fish bodies, and slight in side effect on the environment can be realized, the feed is in accordance with the requirements of green and healthy aquatic products.

The invention provides a preparation method and an application of LcGa recombinant protein of galaptins of larimichthys crocea, and relates to genetic engineering. The preparation method comprises steps as follows: 1), a pGEX-6P1-LcGa recombinant expression vector is constructed; 2), an escherichia coli expression strain pGEX-6P1-LcGa-BL21 with high-copy transformants is acquired; 3), fermented cultivation and induced expression of the transformants are performed; the recombinant protein LcGa is purified. The LcGa recombinant protein of galaptins of the larimichthys crocea can be applied to mediated bacteria agglutination and mediated red blood cell agglutination, and can be applied to preparation of fish immune additives and bacterial control drugs. The prokaryotic expression recombinant protein exists in a soluble mode, and the natural bioactivity of the recombinant protein is kept; further, the preparation method is simple and easy to operate, the yield is high, the low-cost scale production becomes possible, and a new way is provided for development of the novel fish immune additives and the bacterial control drugs.

The invention discloses a method for preparing collagen polypeptides by using larimichthys crocea leftovers. The larimichthys crocea leftovers are fermented by condensed bacillus to obtain collagen polypeptide fermented substances, and the molecular weight of a product is 1,000-3,000 daltons; the larimichthys crocea leftovers comprise fish scales, fish skins and viscera mixtures; the preservation number of the condensed bacillus is CCTCC No:M2013193. According to the method, main nutritional substances produced by fermenting the larimichthys crocea leftovers by the condensed bacillus are free calcium and the collagen polypeptides; acidic products and the calcium which are produced in a fermenting process can be converted into soluble calcium; furthermore, extracellaluar proteases can be used for hydrolyzing proteins in the larimichthys crocea leftovers to supply the collagen polypeptides, ammonia acid, phosphorus and the like which assist in calcium absorption.

The invention relates to a vibration classifier for processing larimichthys crocea. The vibration classifier comprises an eccentric wheel mechanical vibration device, a barrel-shaped material receiver, fish body classifying tank bodies for distinguishing small-size fish bodies from medium-size fish bodies and large-size fish bodies, and discharging conveyors corresponding to the fish body classifying tank bodies for distinguishing small-scale fish bodies from medium-scale fish bodies and large-scale fish bodies. An orientating and classifying long tank body is sequentially provided with a fish body head-tail orientating segment tank body, a fish body turning side-standing declining chute segment tank body, a fish body side-erecting sliding segment U-shaped tank body, a small-size fish body classifying segment tank body, a medium-size fish body classifying segment tank body and a large-size fish body classifying segment tank body. The barrel-shaped material receiver and the front end of the orientating and classifying long tank body are in gap connection. After raw material fish are fed into the barrel-shaped material receiver, under the combined action of a rotating material plate and a vibrating device, the raw material fish slides into the orientating and classifying long tank body, the situations that fish bodies lie sideways and go forwards in a manner that fish heads are at the front part and fish tails are at the rear part, the fish bodies turn, side-stand and slide, and the fish bodies do not overlap, step, side-stand and go forwards are sequentially realized, and when the fish bodies in array pass through the small-size fish body classifying tank body, the medium-size fish body classifying tank body and the large-size fish body classifying tank body, the fish bodies separately fall into the below corresponding small fish body discharging conveyor, the medium fish body discharging conveyor and the large fish body discharging conveyor, so that multiple-size classified treatment of the larimichthys crocea in batch is realized.

The invention relates to an aquatic feed additive, in particular to a method for improving the survival rate of Larimichthys crocea larvae, a feed, a preparation method and application thereof. The method comprises that the starch content in the feed for Larimichthys crocea larvae is reduced and the fat content is increased to make the starch content <=6%and fat content >=15% in the feed; preferably, the starch content is 5-6% and the fat content is 15-16% in the feed for Larimichthys crocea larvae. The feed for Larimichthys crocea larvae provided by the invention has the advantages that, based on the demand of Larimichthys crocea larvae for the energy substance, the nutrition control method is adopted, and the test proves that the feed can enhance the fish body constitution, accelerate the ingestion speed, improve the survival rate, promote growth, and improve seedling raising efficiency of Larimichthys crocea larvae.

The present invention discloses a method for preparing functional minced larimichthys crocea products by using mixed microbe fermentation. The method mainly includes the following steps: monascus and lactic acid bacteria are added in the chopped minced larimichthys crocea, and the mixture is subjected to co-fermentation to obtain the functional minced larimichthys crocea products. The functional minced larimichthys crocea products prepared by the co-fermentation of the monascus and lactic acid bacteria have good flavor and gel characteristics, and solve the problems that the existing minced larimichthys crocea products are heavy in fishy smell, bad in flavor, etc. The method develops the applied food microorganisms in the processing of the fish products, also develops the new minced fish products, and is conducive to improving the technical level and the added value of the products of aquatic product processing.

The invention relates to the technical filed of aquatic product processing, and specifically relates to a drying treatment method of pseudosciaena crocea. The drying treatment method of the pseudosciaena crocea comprises the following steps: (1), carrying out pre-treatment, namely removing viscera and gills of freshly fished pseudosciaena crocea or thawed frozen pseudosciaena crocea, thoroughly washing surface and inside of the pseudosciaena crocea by using washing liquid, and carrying out rapid draining; (2), carrying out soaking, namely putting the pre-treated pseudosciaena crocea into a soaking solution, carrying out ultrasound-aided soaking at 30-40 DEG C for 15-60 minutes, and fishing the soaked pseudosciaena crocea out so as to obtain a material ready for using; (3), carrying out surface treatment, namely coating or spraying on the surface of the pseudosciaena crocea so as to form a dry oil layer; and (4), carrying out drying, namely carrying out fast drying at 100-120 DEG C for 0.5-1 minutes, carrying out drying by using circulating hot air at 50-60 DEG C for 1-2 hours, and carrying out drying at 20-30 DEG C for 2-3 days. The method is used for carrying out drying treatment on the pseudosciaena crocea so as to facilitate storage of the pseudosciaena crocea. The method is short in treatment time, and is capable of relatively well preserving color and luster of the pseudosciaena crocea; moreover, meat color of the pseudosciaena crocea does not become dark during the treatment processes.

The invention relates to a cross method between large yellow Crocker and GuangDong large yellow Crocker. Wherein, their crossed generation has high grow speed and the application in long transmission; the invention selects GuangDong large yellow crocker as father, whose belly can be pressed to flow sperm; preparing dilute solution; compressing its belly to absorb sperm, whose activity is higher than 90% to be frozen and stored; mixing the dilute solution and anti-freeze agent, adding sperm, storing in frozen storage tube, sealing and putting in 4Deg. C; arranging it above the liquid nitrogen, reducing temperature and moving it into liquid nitrogen; selecteing the wide GuangDong large yellow crocker as mother; when its ovogen is mature, defreezing the sperm, and taking the freeze storage tube from liquid nitrogen to be put into room-temperature fresh water to be defrozen, activating it with seawater; artificial inseminating and washing zygote with seawater, removing sink zygote, and charging air and cultivating.