251 results about "Larimichthys crocea" patented technology

The invention provides a large yellow croaker medium-low density SNP liquid phase chip and application thereof, the chip is prepared from a probe for detecting 15,292 SNP molecular marker combinations of a large yellow croaker whole genome, and the position and base information of each SNP site on the chip are shown in a table 1. The invention further provides a preparation method and application of the chip, such as application in large yellow croaker population genetic diversity analysis, breeding population genotype identification, character correlation analysis or genome selective breeding. The chip designed by the invention comprises a series of core sites obviously related to growth, flow resistance and quality characters, and also comprises a large number of background sites, so that the detection accuracy of the designed chip is guaranteed, and the chip has the obvious advantage of low detection cost, the detection cost is reduced by more than 30%, and the detection efficiency is improved. The scientific and technological innovation of the large yellow croaker dark blue breeding industry can be further accelerated.

The invention discloses SYBR Green I real-time fluorescent quantitative PCR (polymerase chain reaction) detection primers and a kit for large yellow croaker pyramidal worm, and belongs to the technical field of biotechnology and diagnostic detection. A pair of specific primers designed by the invention comprises an upstream primer as shown in SEQ ID NO. 1 and a downstream primer as shown in SEQ ID NO. 2. By adopting the kit disclosed by the invention, the rapid quantitative detection of the large yellow croaker pyramidal worm can be realized, the detection specificity is strong, and the sensitivity is high. At present, a research report of a real-time fluorescent quantitative PCR detection method for quantitatively detecting the Pseudosciaena crocea pyramidal based on SYBR Green I does not exist, a basis can be provided for diagnosis of Pseudosciaena crocea pyramidal infection, an important role is played in guarantee of development of Pseudosciaena crocea breeding industry, and the method has a good application prospect.

The invention discloses a method for obtaining high-quality fertilized eggs of wild sal-family pseudosciaena crocea, and belongs to the technical field of aquatic animal breeding. The method comprises the following steps: capturing wild parent fishes in the breeding season of the sal-family pseudosciaena crocea and transporting the wild parent fishes to a farm; the method comprises the following steps: carrying out temporary culture and intensive culture on wild parent fishes, selecting male and female wild parent fishes with mature gonads, anesthetizing, and injecting artificial spawning induction mixed hormones; after artificial spawning mixed hormones are injected, ova and seminal fluid are collected, and high-quality fertilized ova are obtained through artificial insemination. According to the method, all the technical steps are associated with one another to form a whole, the high-quality fertilized eggs of the large yellow croakers of the wild sal ammoniac family can be successfully obtained, and the proportion of the obtained high-quality fertilized eggs reaches 45% or above. According to the method, the obtained high-quality fertilized eggs are adopted for fry hatching, the hatching rate of the obtained fertilized eggs reaches 62% or above, the fertilized eggs can be used for constructing and breeding a wild sal-family pseudosciaena crocea basic population, and artificial breeding of the sal-family pseudosciaena crocea is achieved.

The invention discloses a large yellow croaker fry enhancement and release wild training device and method, and relates to the technical field of marine biological resource conservation, and the large yellow croaker fry enhancement and release wild training device comprises a water tank for containing water, and a plurality of fish reefs are placed in the water tank; the light source is arranged above the water tank; the plurality of wave making pumps are uniformly arranged on one side of the inner wall of the water tank; the throwing structure is used for throwing natural enemy pheromones into the water tank; the camera is arranged on the inner wall of the water tank; the controller is electrically connected with the camera, the light source and the wave making pumps, the camera is used for collecting behavior characteristic information of the large yellow croaker in wild training and sending the behavior characteristic information to the controller, and the fish behavior analysis system comprehensively analyzes swimming and fish reef use conditions of the large yellow croaker fry in the water tank and analyzes and judges the wild training effect of the large yellow croaker fry. The method has the advantages that the capability of the larimichthys crocea fry adapting to the marine natural environment is enhanced, and the survival rate of the released fry is increased.

The invention belongs to the technical field of molecular identification of aquatic organisms, and particularly relates to a high-sensitivity large yellow croaker germplasm identification primer combination as well as application and an identification method thereof. The primer composition is a first pair of primers LC-1, and the sequences of the first pair of primers LC-1 are as follows: an upstream primer LC-1F: 5 '-AATTCCACACGACTAATC-3'; the downstream primer LC-1R is 5 '-CTCAGCCGCCGTGCAATC-3', and the downstream primer LC-1R is 5 '- The sequences of the second pair of primers LC-2 are as follows: an upstream primer LC-2F: 5 '-AGGCATTAGGCACAAGG-3', a downstream primer LC-2F: 5 '-AGGCATTAGGCACAAGG-3', a downstream And the downstream primer LC-2R is 5 '-AAACCCCTAGCCTCGAAG-3'. The invention also discloses a kit for detecting the disease resistance of the traditional Chinese medicine. The two large yellow croaker germplasm identification primers provided by the invention have relatively high sensitivity and specificity, can be stably amplified in a DNA sample with extremely low concentration, and overcome the limitation that accurate identification cannot be realized due to low DNA concentration in a traditional method.

The invention provides a large yellow croaker feed processing and mixing method and device. The method provided by the invention comprises the following steps: determining a growth stage of the large yellow croaker; determining formula data of the large yellow croaker based on the growth stage; the intelligent control system determines processing parameters of the processing mixing system based on the formula data; the change state of the raw materials in the processing and mixing system is monitored in real time based on a sensor; the intelligent control system optimizes formula data of the pseudosciaena crocea and processing parameters of the processing and mixing system in real time according to the change state; and processing and mixing according to the formula data and the processing parameters of the processing and mixing system to obtain the large yellow croaker feed. According to the method and device provided by the invention, the formula data and the corresponding processing parameters are adjusted in time according to the growth stage and actual requirements of the large yellow croaker, accurate feeding of the feed for the large yellow croaker is realized, and growth and health of the large yellow croaker are guaranteed.

The invention relates to the field of aquaculture, in particular to a preparation method and application of a larimichthys crocea iridovirus recombinant live vector vaccine. According to the invention, replication-deficient type 5 adenovirus is selected as a target gene expression vector, a recombinant adenovirus vector comprising main capsid protein of iridovirus is constructed, recombinant virus is obtained, and the safe and effective large yellow croaker iridovirus disease recombinant live vector vaccine is created. According to the vaccine, the larimichthys crocea is immunized in a soaking or bait feeding mode, the CPM of the larimichthys crocea immunized by the vaccine is 12.0%, and the CPM of the fish without being immunized by the vaccine is 62.0%. In addition, the RPS of the inoculated fish and the RPS of the empty carrier control group fish are 80.65% and 9.68% respectively. The method is at a leading level in the field of pseudosciaena crocea biomedicine at present, and creativity is remarkable.

The invention belongs to the technical field of fish genetic identification, discloses a multi-site large yellow croaker wild domestication population identification system based on an agarose gel electrophoresis technology, and particularly discloses a group of InDel molecular markers for large yellow croaker wild and domestication population identification, and the InDel molecular markers comprise InDel11-5053756, InDel20-14004876, InDel3-28345321 and InDel21-17327392. The InDel molecular marker detection primer is used for amplification to obtain a PCR product, agarose gel can be used for detecting strip polymorphism, extra restriction enzyme digestion or complicated polyacrylamide gel electrophoresis operation is not needed, and identification of large yellow croaker wild and domesticated populations can be rapidly and accurately achieved.

The invention provides a larimichthys crocea fast-growing character associated SNP marker combination and breeding application, the combination is prepared from a probe for detecting 10 SNP molecular marker combinations of larimichthys crocea fast-growing characters, and information of positions of all SNP molecular markers on chromosomes, alleles with changed sites and reference genes is shown in a table 1. According to the SNP marker combination disclosed by the invention, the optimal large yellow croaker individuals with fast-growing potential and a certain scale can be selected from the breeding offspring in advance for breeding and breed conservation, and the large yellow croaker individuals are bred to the parent without breeding and breed conservation of a large number of offspring seeds in the breeding process, so that the breeding cost can be greatly reduced. The SNP marker combination provided by the invention can solve the problem that non-lethal detection of the growth speed of the large yellow croaker cannot be realized in a traditional breeding mode, and can ensure smooth development of fast-growing character breeding work of the large yellow croaker.

The invention provides a larimichthys crocea iridovirus inactivated vaccine as well as a preparation method and application thereof, and belongs to the technical field of preparation of viral vaccines. The larimichthys crocea iridovirus inactivated vaccine comprises an inactivated larimichthys crocea iridovirus and an adjuvant, and the strain of the larimichthys crocea iridovirus is an FD201807 strain. After the larimichthys crocea iridovirus inactivated vaccine is injected, the phenomena of ingestion and abnormal behaviors of fishes are avoided, the death phenomenon is avoided, the safety is high, the relative immune protection rate of the inactivated vaccine to immunized fishes is 51.20% or above, the immune effect is good, and the larimichthys crocea iridovirus inactivated vaccine can be applied to prevention and treatment of larimichthys crocea iridovirus diseases. The preparation method of the larimichthys crocea iridovirus inactivated vaccine is simple and convenient, easy to operate, low in cost and suitable for industrial production.

The invention relates to the technical field of aquatic product treatment, and discloses a large yellow croaker automatic grading and sorting device based on machine vision, which comprises a conveying system, a recognition and detection system, a control system and a sorting system. According to the large yellow croaker automatic grading and sorting device based on machine vision, the working distance of the camera can be adjusted, adaptive adjustment can be better carried out according to requirements, and in the whole operation process, the problem that cultured fishes are graded by mainly adopting a manual sorting mode at present, and the sorting efficiency is greatly improved is solved. The method has the problems of low efficiency, high labor input, high fish body damage rate and the like, the device can automatically identify the large yellow croaker, automatically measure the size, estimate the weight and the fatness through a visual system, and classify and sort the large yellow croaker, and by setting the classification standard, the sorting efficiency is improved, the input labor cost is reduced, and the product quality is improved. In addition, damage to fish bodies is small, practicability is high, and the requirement for the existing large yellow croaker sorting process is better met.

The invention relates to haplotypes of cobia fatness degree related SNP sites, a primer group and application of the haplotypes and the primer group, and belongs to the technical field of molecular markers, seven SNP sites related to cobia fatness degree are screened, primers for amplifying the SNP sites are provided, breeding of new varieties with fatness degree characters can be assisted by utilizing the SNP sites or the primers, and breeding of cobia fatness degree characters is facilitated. The method can greatly shorten the breeding period, reduce the dependence on phenotypic characters, improve the selective breeding efficiency, and have good application prospects.

The invention provides an artificial breeding method of wild sal-family large yellow croaker, and belongs to the technical field of aquatic animal breeding. The invention provides an artificial breeding method of wild sal-family pseudosciaena crocea, which comprises the following steps: domesticating the wild sal-family pseudosciaena crocea, then carrying out intensive cultivation, and after the wild sal-family pseudosciaena crocea is cultivated until gonad is mature, carrying out artificial spawning induction and natural fertilization on the obtained parent fish of the sal-family pseudosciaena crocea to obtain fertilized eggs; the fertilized eggs are subjected to incubation and fry cultivation, and sal-family large yellow croaker fries are obtained; the artificial spawning method comprises the step of injecting hormone into parent fish in two steps. According to the method, favorable conditions are created for large-scale breeding of the sal-family pseudosciaena crocea, and a large number of artificially bred sal-family pseudosciaena crocea fry groups can be obtained by adopting the method.

The invention provides a breeding method of all-male pseudosciaena crocea and a matched preservation line, and relates to the technical field of pseudosciaena crocea breeding. According to the all-male larimichthys crocea, YY type male larimichthys crocea and XX type female larimichthys crocea are paired, and all produced offspring are normal XY type male larimichthys crocea. The cultivation method of the large yellow croaker matched preservation line comprises the following steps: firstly, performing induced transformation on male large yellow croaker by using estrogen to obtain XY type female large yellow croaker; then the XY type female pseudosciaena crocea subjected to sexual transformation is matched with normal XY type male pseudosciaena crocea, and produced offspring contains YY type male genetic pseudosciaena crocea; and pairing the YY type male genetic large yellow croaker with the normal XX type female large yellow croaker, wherein all the produced offspring are the normal XY type male large yellow croaker. Through the application of estrogen induction and genetic sex identification technologies, the sex control accuracy of the large yellow croaker is realized, and a new technical means is provided for the aquaculture industry.

The invention belongs to the technical field of biology, and particularly relates to application of hepcidin expression quantity in larimichthys crocea liver as larimichthys crocea visceral white-spot disease resistance evaluation index and a construction method. The hepcidin expression quantity in the larimichthys crocea liver is applied as the larimichthys crocea visceral white-spot disease resistance evaluation index. The construction method of the resistance evaluation index comprises the following steps: taking healthy small yellow croaker intraperitoneal injection pseudomonas proteinus strain bacterial liquid, and determining a half lethal dose in 96 hours; the method comprises the following steps: injecting a half lethal dose of bacterial liquid of pseudomonas proteinus strains into the abdominal cavity of healthy small yellow croaker for 96 hours, and after infection, taking different tissues to carry out hepcidin gene expression fluorescent quantitative PCR analysis; a regression model of gene expression quantity and bacterium loading quantity is established through fluorescence quantification, and the relationship between hepcidin expression quantity and disease resistance is constructed. Accurate disease-resistant phenotype information is provided for development of breeding of visceral white-spot disease-resistant improved varieties of the small yellow croakers, the accuracy of disease-resistant breeding is promoted, early detection of diseases is realized, and prevention and treatment of the diseases are effectively guided.

The invention relates to a composition for treating large yellow croaker pyramidoniasis as well as a preparation method and application thereof, and belongs to the technical field of aquaculture. The composition comprises the following components: curcumin, bile acid, beta glucan, bacillus subtilis freeze-dried powder, marine red yeast freeze-dried powder, vitamin C, vitamin E and ethylicin. When the feed additive is added into basal feed to feed large yellow croakers infected with the trypanosomiasis, the death rate of sick fishes can be effectively reduced, the polypide loading capacity is remarkably reduced, and pathological injuries of livers and spleens of the large yellow croakers are relieved. In addition, the composition can also improve the non-specific immunity of the fish body, improve the liver function, enhance the antioxidant capacity of the liver, and improve the lipase activity of the intestinal tract at the same time. Therefore, the composition can be used as a medicine or a feed additive for treating the large yellow croaker pyramidoniasis, has the advantages of small dosage and remarkable effect, and provides a new technical approach for solving the problem that the current large yellow croaker pyramidoniasis lacks an effective prevention and control means.

The invention discloses a large model-based large yellow croaker growth stage feeding amount evaluation method and system, and belongs to the field of aquaculture, and the method comprises the steps: collecting underwater images of a fish pond in a plurality of shooting directions at the same time, and recognizing and segmenting a fish body image corresponding to each entity fish in each underwater image; determining the distance between an entity fish corresponding to the fish body image and a camera for collecting the fish body image, and extracting fish body appearance features of the fish body image; randomly selecting a fish body image from each shooting direction to form a fish body image association combination, and screening a second fish body image association combination from all possible fish body image association combinations according to the distance and fish body appearance features; and constructing three-dimensional point cloud data of the entity fishes according to the second fish body image association combination, fitting three-dimensional body shape parameters of the entity fishes through the three-dimensional point cloud data, and determining the final feeding amount of the fish school according to the three-dimensional body shape parameters of the entity fishes. According to the invention, the problem of low accuracy of the real-time feeding amount of the fish school can be solved.

The invention discloses a larimichthys crocea IL-22 recombinant protein which is a larimichthys crocea IL-22 mature peptide sequence, and the amino acid sequence of the larimichthys crocea IL-22 recombinant protein is as shown in SEQ ID NO. 2. The recombinant protein is obtained by amplifying and coding gene segments of amino acids from 31st to 190th positions of large yellow croaker IL-22 mature peptide through PCR (Polymerase Chain Reaction), and transforming escherichia coli for heterologous expression. The larimichthys crocea IL-22 recombinant protein can activate a transcription factor STAT3 in a larimichthys crocea head kidney cell line (LYCK) and also can induce expression of inflammatory factors (IL-1beta, IL-6 and IL-8) in immune organs of larimichthys crocea, so that the larimichthys crocea IL-22 recombinant protein has an obvious immunoregulation effect and shows a good application prospect in research and development of aquatic immunomodulators.

The invention belongs to the technical field of fish genetic identification, discloses a multi-site sal-family wild large yellow croaker identification method based on an SNaPshot technology, and particularly discloses a group of SNP (Single Nucleotide Polymorphism) molecular markers for screening a sal-family wild large yellow croaker group. The identification of the sal-family wild pseudosciaena crocea in the pseudosciaena crocea wild population sample can be realized only by a simple multiple PCR reaction system and SNaPshot typing sequencing. And automatic typing and identification of the wild large yellow croaker sample of the large sal ammoniac family can be realized only by operating on various genetic analysis instruments. Compared with the existing method for identifying the wild large yellow croaker in the sal family, the method has the advantages that the typing is more accurate, the operation is quicker, the detection flux is higher, and meanwhile, the accuracy rate and the cumulative exclusion rate are higher.

The present invention discloses a low - serum medium and its application in the preparation of cultured meat from Larimichthys crocea cells, which is also applicable to other marine or freshwater fish, belonging to the field of bioengineering technology. The low - serum medium comprises a basal medium, fetal bovine serum accounting for 30 - 80 μL / mL, and exogenous additives. The exogenous additives include human serum albumin, ascorbic acid, basic fibroblast growth factor polypeptide, human holo - transferrin, lysophosphatidic acid, and human recombinant IGF - 1. Based on the DMEM / F12 medium, the low - serum medium provided by the present invention adds six substances that promote cell growth, reduces the dependence of satellite cells on fetal bovine serum, effectively reduces the usage amount of fetal bovine serum, and saves the culture cost. Compared with the general - type medium (DMEM high - glucose, adding 10% FBS), the low - serum medium provided by the present invention has an equivalent effect on the maintenance and proliferation ability of cell stem cells.

The invention discloses a large yellow croaker origin traceability discrimination method based on an element and FTIR fusion fingerprint spectrum. The method comprises the following steps: S1, sample treatment; s2, data acquisition: S2.1, mineral element data acquisition, and S2.2, FTIR spectrum acquisition; and S3, data analysis. Precise classification of production areas can be realized through principal component analysis (PCA), clustering analysis (CA) and linear discriminant analysis (LDA), the overall discriminant accuracy of back substitution inspection of the discriminant model reaches 100%, and the overall discriminant accuracy of cross inspection reaches 97.3%. The element and infrared spectrum fusion fingerprint technology is applied to the source tracing of the large yellow croaker producing area, and the application prospect is wide.

The invention provides a fish source pseudomonas plecoglossicida, which is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC M 2024372. The invention also relates to an application of the fish-derived pseudomonas plecoglossicida in preparation of a vaccine for preventing fish visceral white-spot disease. The invention also relates to a vaccine for preventing the fish visceral white-spot disease, which is characterized by comprising the inactivated fish source pseudomonas plecoglossicida. According to the invention, the growth characteristics and toxicity of the separated Pseudomonas plecoglossicida (ND2304 strain) are determined, and the Pseudomonas plecoglossicida (ND2304 strain) is determined to be a virulent strain with strong pathogenicity to large yellow croaker; the prepared corresponding Pseudomonas plecoglossicida inactivated vaccine can be combined with an adjuvant, and the inactivated vaccine is relatively high in safety and relatively high in relative protection rate.

The invention provides a domestication method of wild sal-family large yellow croaker fries, and belongs to the technical field of aquaculture. The method comprises the following steps: capturing wild fry of the halitium larimichthys crocea, transporting the wild fry to a culture pond for temporary culture, and then sequentially carrying out live bait domestication, chilled bait domestication, soft pellet feed domestication and artificial matched pellet feed domestication culture. According to the method, live baits loved by the wild sal-family pseudosciaena crocea are fed and are gradually transited to special artificial compound feed, so that the feeding habit and digestive system of the wild sal-family pseudosciaena crocea are gradually adapted, the wild sal-family pseudosciaena crocea can be successfully converted to eat the artificial compound feed after being domesticated for 20 days, the survival rate reaches 63% or above, and the phenomenon of batch death does not occur during domestication. According to the method, the survival rate of the fries domesticated from the wild sal-family large yellow croaker fries into the fries under the artificial breeding condition is effectively increased, and a guarantee is provided for building an artificial breeding basic parent fish group.

The invention provides a larimichthys crocea anti-flow character associated SNP marker combination and breeding application, the combination is prepared from a probe of 13 SNP molecular marker combinations for detecting larimichthys crocea muscle elasticity characters, and information of positions, alleles with changed sites and reference genes of all SNP molecular markers on chromosomes is shown in a table 1. According to the SNP marker combination disclosed by the invention, the optimal larimichthys crocea individuals with the anti-flow potential and a certain scale can be selected from the breeding offspring in advance for breeding and breed conservation, and the larimichthys crocea individuals are bred to the parent without breeding and breed conservation of a large number of offspring seeds in the breeding process, so that the breeding cost can be greatly reduced. By utilizing the SNP marker combination, the problem that non-lethal detection of the muscle elasticity of the large yellow croaker cannot be realized in a traditional breeding mode can be solved, and smooth development of anti-flow character breeding work of the large yellow croaker can be ensured.

The invention discloses a method for regulating out-of-season breeding of large yellow croakers bred in autumn. The method is characterized in that spawning in spring and summer is achieved through a three-stage technical system of biological safety prevention and control, precise environment regulation and control and reproductive physiological intervention. The method specifically comprises the following steps: 1) detecting trypanosomes; 2) parent fish screening; (3) parent fish rearing; 4) artificial propagation; 5) artificial incubation; and 6) seedling cultivation. According to the method, the out-of-season spring and summer breeding technology breakthrough of breeding the large yellow croaker in autumn is successfully achieved through nutrient enrichment, accurate temperature parameter regulation and control, gradual illumination regulation and reasonable hormone treatment. According to the technology, an adjustable reproduction synchronization scheme is provided for cross breeding between a spring reproduction group and an autumn reproduction group, and the technology has important application value for germplasm innovation of the large yellow croaker.

The invention provides a method for on-site rapid artificial insemination of wild sal-family large yellow croakers, and belongs to the technical field of aquatic animal breeding. According to the method, wild parent fishes are captured in the breeding season of the sal-family pseudosciaena crocea; selecting sexually mature male parent fishes on a capturing site, and collecting seminal fluid for later use; after manual semen collection is finished, female parent fishes with sexual maturity are selected, and fish eggs are collected; performing artificial insemination to obtain high-quality fertilized eggs; the artificial insemination comprises the following steps: adding seminal fluid into the roes, then adding natural seawater, stirring, and finally washing with the natural seawater. According to the method, key links of the whole artificial insemination process are comprehensively regulated and controlled, so that the purposes of obtaining high-quality fertilized eggs and carrying out artificial fry breeding by utilizing the parent fish of the wild sal ammoniac large yellow croaker are achieved.

The invention discloses a large yellow croaker feeding behavior monitoring method and system based on target detection, and the method comprises the steps: carrying out the feature extraction of a large yellow croaker feeding video collected in real time, obtaining a visual behavior feature, and carrying out the feature extraction of a large yellow croaker acoustic signal collected in real time, and obtaining an acoustic behavior feature; performing time alignment on the visual behavior features and the acoustic behavior features through a preset cross-modal phase synchronization network to obtain a first feature pair, and performing semantic alignment on the first feature pair through a preset attention mechanism to obtain a second feature pair; performing feature fusion on the second feature pair to obtain a multi-modal feature, and outputting an ingestion behavior recognition result according to the multi-modal feature through a preset first ingestion behavior recognition model; and when the ingestion behavior result belongs to the abnormal ingestion behavior category, performing ingestion behavior abnormal early warning according to a preset grading early warning mechanism. The accuracy of large yellow croaker feeding behavior monitoring can be improved.

The invention relates to a large yellow croaker shield ciliate disease pathogen detection primer, a kit and application. The pathogens of the Pseudosciaena crocea shield ciliate disease comprise greedy Michaelia mirata and marine Tylocercidae. Two pairs of specific primers are designed by taking 18S rDNA (ribosomal deoxyribonucleic acid) genes of greedy Michaelike and marine Tylobacter as target genes, and are prepared into a reaction system according to a certain proportion for PCR (polymerase chain reaction) amplification, so that a PCR method capable of simultaneously and rapidly detecting the two pathogens of the Pseudosciaena crocea didysosis is established, and the PCR method has the advantages of strong specificity and high sensitivity. Reliable molecular detection technical support is provided for early rapid diagnosis of the Pseudosciaena crocea shield ciliate disease.

This invention discloses a device and method for the propagation, release, and rewilding training of large yellow croaker fry, relating to the field of marine biological resource conservation technology. The device includes: a water tank containing water, with multiple artificial reefs placed inside; a light source positioned above the water tank; multiple wave-making pumps evenly distributed along one side of the inner wall of the water tank; a release structure for releasing predator pheromones into the water tank; a camera mounted on the inner wall of the water tank; and a controller electrically connected to the camera, light source, and wave-making pumps. The camera collects behavioral characteristic information of the large yellow croaker during rewilding training and sends this information to the controller. A fish behavior analysis system comprehensively analyzes the swimming and reef usage of the large yellow croaker fry in the water tank to determine the effectiveness of the rewilding training. The advantages of this invention are enhanced ability of large yellow croaker fry to adapt to the marine environment and improved survival rate of released fry.

This invention discloses a multi-unit composite stress tiered breeding device and method for juvenile large yellow croaker, belonging to the field of aquaculture breeding technology. The device uses a central control unit as its sole core, forming a fully integrated unidirectional closed-loop drive system. Four independent breeding units are connected through a transparent channel with a centrally located aeration head, forming a physical flow-limiting isolation structure. Each unit independently regulates water temperature, salinity, and dissolved oxygen, creating a multi-factor composite stress gradient. The breeding method employs a three-factor linear equal-gradient synergistic progressive setting, utilizing the juvenile fish's autonomous adaptation and avoidance instincts to achieve cross-unit migration and natural selection. Combined with contour recognition and dynamic tracking algorithms, it monitors the fish population status and mortality rate in real time, and identifies superior offspring with comprehensive stress resistance through secondary verification under extreme stress. This invention offers high screening accuracy and automation, increasing the survival rate of superior individuals by over 40%, reducing labor costs by over 60%, and allowing for flexible adjustment of device parameters. It can be extended to the stress resistance breeding of other marine economic fish species.