7 results about "Head Kidney" patented technology

The invention provides a larimichthys crocea head kidney macrophage line as well as a construction method and application thereof, belongs to the technical field of cell engineering, and particularly provides the larimichthys crocea head kidney macrophage line which is named as LYC-hK and has the preservation number of CCTCC NO: C2025234. The larimichthys crocea head kidney macrophage line is constructed through the steps S1-S6, and the construction method of the larimichthys crocea head kidney macrophage line is applied to the field of cell engineering. The larimichthys crocea head kidney macrophage line provided by the invention can be used for research on pathogenic infection and pathogenic mechanism of larimichthys crocea, research on anti-infection immune response of larimichthys crocea and research on exogenous gene expression.

The present application belongs to the technical field of biological detection, and particularly relates to a detection reagent and a detection kit for identifying sub-dong salmon resistant to bacterial gill disease. The detection reagent for identifying sub-dong salmon resistant to bacterial gill disease is obtained by performing transcriptome sequencing on the head kidney of 6 susceptible and 6 resistant sub-dong salmon, screening out 104 SNP specific sites, performing multiple PCR post-genomic sequencing, and analyzing and population verifying the SNP sites through susceptible and resistant sub-dong salmon samples. Six SNP specific sites are screened out. The six SNP sites are used as detection items of the kit for screening sub-dong salmon resistant to bacterial gill disease. The reagent and the kit have the advantages of short library construction cycle, good repeatability, simple operation, etc. The sub-dong salmon resistant to bacterial gill disease can be quickly screened without damaging the sub-dong salmon, so as to quickly form a sub-dong salmon breeding population resistant to bacterial gill disease.

The invention discloses an application of Harman alkali in the aspect of preparing a Pseudomonas plecoglossicida inhibitor, and is characterized in that the Harman alkali is applied in the aspect of preparing a medicine for preventing and / or treating aquatic animal infectious diseases caused by Pseudomonas plecoglossicida. The invention further provides application of the harman alkali or the pharmaceutically acceptable salt thereof in preparation of a medicine for preventing and treating aquatic animal plecoglossus altivelis pseudomonas infection, application of the harman alkali or the pharmaceutically acceptable salt thereof in preparation of a medicine for preventing and treating plecoglossus altivelis liver injury, application of a plecoglossus altivelis immunoregulation medicine and application of the harman alkali or the pharmaceutically acceptable salt thereof in preparation of an aquaculture feed additive. The method has the advantages that the survival rate of aquatic animals can be remarkably increased in a Pseudomonas plecoglossicida infection model, the bacterial load in immune tissues such as blood, liver, spleen, head and kidney and the like is reduced, and liver and spleen tissue pathological injury and cell apoptosis caused by infection are relieved; meanwhile, harmine can improve biochemical and antioxidant indexes of infection-related serum, and has no obvious toxicity to fish-derived cells within a certain concentration range.

This invention relates to a fish interferon regulator IRF4 gene, protein, expression vector, and their applications, belonging to the field of fish molecular immunology technology. The fish interferon regulator IRF4 is derived from the oval pomfret, and the cDNA nucleotide sequence of the oval pomfret IRF4 gene is shown in SEQ ID NO.1. The amino acid sequence of the protein encoded by the oval pomfret IRF4 gene is shown in SEQ ID NO.2. This invention also provides an expression vector containing the oval pomfret IRF4 gene, and an anti-Vibrio harveyi infection preparation containing the protein or a eukaryotic expression vector. The oval pomfret IRF4 protein of this invention can promote the respiratory burst activity and phagocytic capacity of oval pomfret head kidney macrophages. Overexpression in oval pomfret... Believe After IRF4 is activated, the body's antibacterial ability can be significantly improved, and it can be used as an immune enhancer.

The invention discloses an application of arginine in promoting micropterus salmoides to resist nocardia seriolae infection, which is characterized in that the arginine is used as a functional additive to feed the micropterus salmoides for 8 weeks, then a challenge experiment is carried out, and the influence of the arginine on the nocardia seriolae infection resistance of the micropterus salmoides is explored; comprising high-throughput omics conjoint analysis, mTORC1 pathway activity, antibody content, cell death, tissue structure, survival rate and the like. Researches find that arginine significantly activates an mTORC1 signal channel of micropterus salmoides, and promotes secretion of an IgM antibody in serum to resist nocardia seriola infection; the arginine also inhibits cell death caused by excessive activation of NLRP3 inflammasomes of the micropterus salmoides, so that the micropterus salmoides keeps complete liver, head-kidney and body-kidney structures after being infected by nocardia seriola, and finally the survival rate of the micropterus salmoides after being infected by nocardia seriola is improved. Therefore, the arginine can be used as a functional feed additive for enhancing the capability of the largemouth bass for resisting nocardia seriolae infection.

The present invention discloses ToNK-lysin, an antimicrobial peptide from Takifugu obscurus, whose amino acid sequence is shown in SEQ ID NO. 1. The present invention utilizes in vitro recombinant expression technology to obtain ToNK-lysin, an antimicrobial peptide from Takifugu obscurus. This recombinant protein has no cytotoxic effect on pufferfish head kidney cells and can significantly eliminate Vibrio harveyi in pufferfish. Furthermore, the ToNK-lysin recombinant protein can degrade the genomes of Escherichia coli and Vibrio harveyi and significantly inhibit their growth. As an effective immune effector molecule, ToNK-lysin has potential applications in the development of antimicrobial drugs, new immune preparations, and feed additives.

The invention relates to the field of molecular biotechnology and genetic engineering, and discloses a miR-301a simulant for inhibiting IL-4 / 13A gene expression of large yellow croaker and application of the miR-301a simulant. The miR-301a simulant comprises a double-stranded RNA (Ribonucleic Acid); wherein the sequence of the positive-sense strand of the double-stranded RNA is 5 '-GUGCAAUAGUAUUGUCAAAGCA-3', and the sequence of the antisense strand of the double-stranded RNA is 5 '-UGCUUUGCAAUACUUUGCAC-3'. Researches find that the mRNA expression level of the IL-4 / 13A gene of the pseudosciaena crocea can be remarkably reduced, T and B lymphocyte proliferation of the pseudosciaena crocea can be inhibited and adaptive immune response of the pseudosciaena crocea can be weakened by transfecting the miR-301a simulant in head kidney leukocytes of the pseudosciaena crocea. Therefore, the miR-301a simulant can be used for related research on IL-4 / 13A and adaptive immune response of the large yellow croaker, and has potential application value.