10 results about "Trypanosomiasis" patented technology

The invention relates to a composition for treating large yellow croaker pyramidoniasis as well as a preparation method and application thereof, and belongs to the technical field of aquaculture. The composition comprises the following components: curcumin, bile acid, beta glucan, bacillus subtilis freeze-dried powder, marine red yeast freeze-dried powder, vitamin C, vitamin E and ethylicin. When the feed additive is added into basal feed to feed large yellow croakers infected with the trypanosomiasis, the death rate of sick fishes can be effectively reduced, the polypide loading capacity is remarkably reduced, and pathological injuries of livers and spleens of the large yellow croakers are relieved. In addition, the composition can also improve the non-specific immunity of the fish body, improve the liver function, enhance the antioxidant capacity of the liver, and improve the lipase activity of the intestinal tract at the same time. Therefore, the composition can be used as a medicine or a feed additive for treating the large yellow croaker pyramidoniasis, has the advantages of small dosage and remarkable effect, and provides a new technical approach for solving the problem that the current large yellow croaker pyramidoniasis lacks an effective prevention and control means.

The invention provides application of naphthoquinone compounds in preparation of insecticides for preventing and / or treating parasitic diseases in aquatic animals, and belongs to the technical field of aquaculture. The naphthoquinone compound disclosed by the invention is 2-chloro-1, 4-naphthoquinone, and can be used for killing or preventing pyramidal bodies in aquatic animals. The invention also provides an insecticide for killing or preventing pyramidal worms in aquatic animals by using the naphthoquinone compound, and the insecticide comprises the following raw materials in percentage by weight: 20-30% of the naphthoquinone compound, 5-10% of a surfactant, 5-10% of a dissolving agent and the balance of water. An in-vitro insecticidal experiment and an in-vivo insecticidal experiment prove that the 2-chloro-1, 4-naphthoquinone can be used for efficiently killing insects; and the 2-chloro-1, 4-naphthoquinone has no obvious toxic or side effect on the fish body, and does not influence the growth and physiological functions of the fish body.

The present invention provides diazo compounds having anti-trypanosomal activity, with less or no side-effects. The diazo compounds are structural analogue of quinapyramine and possess characteristics necessary for the targeted pharmacologic action of anti trypanosomosis, with reduced cytotoxicity. The compounds were found to have less cytotoxicity, and identical or better anti- parasitemia effect as compared to conventional quinapyramine sulfate.

The present invention relates to a method for the production of a (poly)hydroxylated pentacyclic triterpene composition including a 3-O-p-coumaroyl ester of tormentic acid from a plant suspension cell culture, to a pharmaceutical composition comprising at least 3-O-p-coumaroyl ester of tormentic acid for a use in the prevention and / or the treatment of trypanosomiasis, optionally in admixture with other (poly)hydroxylated pentacyclic triterpenes, and to 3-O-p-coumaroyl ester of tormentic acid for its use as an antiparasitic agent for the prevention and / or the treatment of trypanosomiasis, optionally in admixture with other (poly)hydroxylated pentacyclic triterpenes.

The application discloses a LF-RPA rapid detection kit for trypansomatosis of large yellow croaker, a use method and application thereof, and belongs to the technical field of gene detection.The application provides a LF-RPA rapid detection kit for trypansomatosis, which comprises a LF-RPA reaction system, freeze-dried powder, a buffer and a test strip.The LF-RPA reaction system comprises the following components in volume fractions: 30 parts of Rehydration buffer, 1-2.5 parts of an upstream primer, 1-2.5 parts of a downstream primer, 0.5-1 part of a probe, 2.5 parts of an L activator, 1-5 parts of a template, and sterilized ultrapure water is added to supplement the volume of the LF-RPA reaction system to 50 parts.The LF-RPA detection method of the kit is simple and convenient to operate, the test strip detection result is intuitive, and professional technical personnel are not needed;expensive PCR reaction instruments are not needed, so that the cost is reduced;the detection speed is fast;the sensitivity is high;and the specificity is good.

The invention provides an RPA (recombinase polymerase amplification) detection kit, an RPA-LFD (lateral flow dipstick) detection system and an RPA-LFD detection method. The sequences of an upstream primer and a downstream primer used in the detection are respectively shown as SEQ ID NO. 1 and SEQ ID NO. 2; the sequence of the probe is as shown in SEQ ID NO.3, and tetrahydrofuran is inserted into the probe. The invention further provides a trypanosome RPA (recombinase polymerase amplification) detection kit and a trypanosome RPA-LFD (lateral flow dipstick) detection system containing the detection kit, and also provides a corresponding detection method. The whole detection process can be completed within 23 minutes, the operation is simple and convenient, and the result is visible. Compared with an existing detection method, the method has the advantages of being rapid, sensitive, high in specificity, suitable for on-site operation and the like, and is suitable for early diagnosis and epidemiological investigation of the trypanosomiasis in the large yellow croaker culture process.

The application belongs to the technical field of biotechnology, and particularly relates to a recombinant antigen of American trypanosomiasis and a preparation method and application thereof. The application provides a recombinant antigen of American trypanosomiasis, wherein a plurality of neutralizing epitopes and complete amino acid sequences of Trypanosoma cruzi antigens are connected according to a specific order to construct a recombinant antigen capable of covering all stages of the life cycle of Trypanosoma cruzi. The recombinant antigen is complete in Chagas antibody recognition site coverage, can recognize and combine all types of antibodies in the Chagas chronic and acute infection period, and is not easily interfered by other substances in serum, and is high in specificity.

The present invention provides oxaborole ester compounds and compositions thereof which are useful to treat diseases associated with parasites, such as Chagas Disease and African Animal Trypanosomosis.

The invention discloses a metronidazole compound preparation for treating fish pyramidoniasis and application of the metronidazole compound preparation, and particularly relates to the technical field of parasitic disease prevention and control. The metronidazole compound preparation comprises metronidazole and a Chinese herbal medicine synergistic extract, wherein the Chinese herbal medicine synergistic extract is a mixture of a wormwood extract and a herba artemisiae scopariae extract. Preparing a metronidazole compound preparation: mixing the wormwood extract and the oriental wormwood extract; crushing metronidazole into micro powder; adding water into pharmaceutic adjuvants to prepare a bonding solution; uniformly mixing the extract powder, the metronidazole micro powder, the slow-release carrier and part of the medicinal carrier, spraying a bonding solution, granulating and drying; and uniformly mixing the granules with the residual medicinal carrier to obtain the pharmaceutical composition. According to the metronidazole compound preparation, through the synergistic interaction of the plant source preparation and the metronidazole, the curative effect of the metronidazole in the metronidazole compound preparation is greatly improved, the effective use dosage is reduced, and meanwhile, the metronidazole compound preparation also has the function of assisting in repairing tissue damage caused by infection of a host.

The present invention relates to antisense oligonucleotides that are capable of reducing expression of PD-L1 in a target cell. The oligonucleotides hybridize to PD-L1 mRNA. The present invention further relates to conjugates of the oligonucleotide and pharmaceutical compositions and methods for treatment of viral liver infections such as HBV, HCV and HDV; parasite infections such as malaria, toxoplasmosis, leishmaniasis and trypanosomiasis or liver cancer or metastases in the liver using the oligonucleotide.