6 results about "Polypide" patented technology

The invention discloses a method for detecting and identifying two insects in water based on deep learning, and relates to the technical field of aquatic biological detection.The method comprises the steps that insect bodies are enriched through a micro-fluidic chip, DAPI / PI dyeing is automatically completed, and recognizable biochemical fluorescence characteristics are given to the insect bodies; dAPI, PI and bright field multichannel images are acquired through a CMOS camera, and sub-pixel-level alignment of multispectral images is realized by using an image registration and fusion algorithm; denoising and segmenting the multispectral image, extracting an insect body area and enhancing the contrast; the positions of polypide bodies are detected through an improved YOLOv8 model, and the activity states of the polypide bodies are judged in combination with fluorescence characteristics and form scores; counting the number of insect bodies and the live-to-dead ratio in real time, identifying capsules, and triggering live insect standard exceeding alarm and pollution trend analysis; a model is deployed on an edge device, an image uploading result is compressed, and data security and traceability are ensured through a block chain. The system integrates sample processing, imaging acquisition, intelligent identification and data feedback, and is suitable for long-time stable operation on site.

The invention relates to an in-vitro culture method and application of anerennial crabs, and belongs to the technical field of aquaculture. The invention provides a culture medium. The culture medium comprises hemolymph of healthy animals and sterile seawater, the volume ratio of the hemolymph of the healthy animal to the sterile seawater is (1-5): (5-9). According to the in-vitro culture method of the crabbidae, the survival time of the crabbidae inoculated into the culture medium for one time is not less than 1 month under the culture system, and when the crabbidae body reaches the optimal state, the culture is transferred to the condition of 2-8 DEG C, so that the survival time of the crabbidae body can be prolonged to be not less than 2 months, and the survival time of the crabbidae body can be prolonged to be not less than 2 months. If the strains are continuously transferred to a fresh culture medium, long-term stable passage of the polypide can be realized, the in-vitro culture system can effectively guarantee supply of the polypide in non-epidemic seasons, and a key technical support is provided for accelerating research and development of quasi-amarerenosis drugs and realizing effective prevention and control of the quasi-amarerenosis.

This invention relates to the field of aquaculture technology, and in particular to an immune enhancer for juvenile large yellow croaker (Culter alburnus), its preparation method, and its application. The immune enhancer provided by this invention, through the compounding of specific components in a specific ratio, achieves the synergistic effect of polypeptides, immunopolysaccharides, antioxidants, and nutritional factors, while also considering rapid absorption, immune regulation, and high-temperature environmental stability. It is suitable for the immune enhancement needs of large yellow croaker juveniles under high-temperature seawater conditions of 29-31 °C, and can enhance the non-specific and specific immunity of juveniles under high-temperature seawater conditions, improving disease resistance and survival rate.

The invention provides an anisakis quarantine method, which belongs to the technical field of parasite quarantine, and comprises the following steps: S1, sample treatment: placing fish tissues in a stationary liquid for fixation; s2, extracting total DNA: after soaking in a TE buffer solution, extracting genome DNA of the polypide, and storing at-15 to-25 DEG C; s3, designing primers, namely designing and synthesizing an upstream primer Anisakis1 and a downstream primer Anisakis2; s4, carrying out PCR (Polymerase Chain Reaction) amplification, namely carrying out PCR amplification on the polypide genome DNA by using the primer; s5, result judgment: performing result judgment on the target amplified fragment through electrophoresis detection; s6, insect species identification: carrying out real-time PCR detection on the positive sample; and S7, judging a result: judging according to a Ct value and an amplification curve. The anisakis quarantine method provided by the invention not only can determine whether the anisakis belongs to anisakis, but also can determine which anisakis specifically carried.

The present invention relates to a characteristic polypeptide of the multispined sea dragon, its application, and a method for identifying the multispined sea dragon, belonging to the field of biotechnology detection technology. The present invention provides two characteristic polypeptides unique to the multispined sea dragon, the amino acid sequences of the characteristic polypeptides being shown as SEQ ID NO.1 and SEQ ID NO.2. The characteristic polypeptides provided by the present invention have excellent specificity and stability for the multispined sea dragon, have high specificity, can be used for the identification of the multispined sea dragon, and have good application prospects.

The invention discloses a method for determining the content of corydalus cornutus polypeptide, which belongs to the technical field of biology, and comprises the following steps: detecting and determining corydalus cornutus characteristic polypeptide sequences as LIRF and LEI by adopting mass spectrometry, and naming the corydalus cornutus characteristic polypeptide sequences as PSC-1 and PSC-2; and preparing a corydalus cornutus solution, preparing a reference substance solution, establishing a standard curve and determining the polypeptide content. The method for determining the content of the polypeptide LIRF and the polypeptide LEI in the corydalus cornutus is established on the basis of the LC-MS technology, has the characteristics of easiness in reference substance synthesis, simplicity in operation, high sensitivity, accuracy in determination and the like, can be used for quality evaluation research of the corydalus cornutus, and provides reference for quality control of the corydalus cornutus.