2 results about "Polypide" patented technology

This invention relates to the field of aquaculture technology, and in particular to an immune enhancer for juvenile large yellow croaker (Culter alburnus), its preparation method, and its application. The immune enhancer provided by this invention, through the compounding of specific components in a specific ratio, achieves the synergistic effect of polypeptides, immunopolysaccharides, antioxidants, and nutritional factors, while also considering rapid absorption, immune regulation, and high-temperature environmental stability. It is suitable for the immune enhancement needs of large yellow croaker juveniles under high-temperature seawater conditions of 29-31 °C, and can enhance the non-specific and specific immunity of juveniles under high-temperature seawater conditions, improving disease resistance and survival rate.

The invention provides an anisakis quarantine method, which belongs to the technical field of parasite quarantine, and comprises the following steps: S1, sample treatment: placing fish tissues in a stationary liquid for fixation; s2, extracting total DNA: after soaking in a TE buffer solution, extracting genome DNA of the polypide, and storing at-15 to-25 DEG C; s3, designing primers, namely designing and synthesizing an upstream primer Anisakis1 and a downstream primer Anisakis2; s4, carrying out PCR (Polymerase Chain Reaction) amplification, namely carrying out PCR amplification on the polypide genome DNA by using the primer; s5, result judgment: performing result judgment on the target amplified fragment through electrophoresis detection; s6, insect species identification: carrying out real-time PCR detection on the positive sample; and S7, judging a result: judging according to a Ct value and an amplification curve. The anisakis quarantine method provided by the invention not only can determine whether the anisakis belongs to anisakis, but also can determine which anisakis specifically carried.