79 results about "Polypide" patented technology

The invention belongs to the field of environmental protection, relating to a method for processing food waste by using black soldier fly larvae and comprising the following steps: coarse crushing the food waste and then adding auxiliary materials to obtain culture materials for the black soldier fly larvae; and grafting black soldier fly spawn on the surface of the culture materials for the black soldier fly larvae so that the larvae hatched by the black soldier fly spawn acquires the culture materials for the black soldier fly larvae, thereby processing and cleaning the food waste. Before the black soldier fly larvae ages, a polypide collecting container is externally connected to the area for piling up the culture materials for the black soldier fly larvae and is filled with dry rice bran powder so as to guide to collect the aged black soldier fly larvae; and the rest of the processed culture materials for the black solider fly larvae is dried and then prepared into microbial organic fertilizer. The invention has strong processing capability, rapid processing speed compared with the common compost, the processing period is 4-5 days; after being processed, the volume of the food waste is reduced by 10-30%, the peculiar smell of the waste is lightened; and the invention can obtain excellent microbial organic fertilizer and insect bioprotein at the same time.

The invention discloses a preservation method of cordyceps sinensis, which comprises the steps of sequentially cleaning the head part, stroma and polypide of the cordyceps sinensis by use of linear running water; soaking the cleaned cordyceps sinensis in a 0.9% acid sodium chloride for 5-8 minutes, and draining; performing quick cooling treatment on the cordyceps sinensis for 5-12 hours at -75 DEG C; putting the cordyceps sinensis after the quick cooling into a vacuum refrigeration box at (-5)-(-25) DEG C, and starting the refrigeration gas self-control equipment in the refrigeration box, wherein the refrigeration gas self-control equipment controls a gas pump to introduce and/or extract oxygen, nitrogen and carbon dioxide gas into/out of the refrigeration box. By adopting the preservation method disclosed by the invention, the cordyceps sinensis can meet the sanitary requirement of direct eating; after long-time storage, the nutritional ingredients and activity of the cordyceps sinensis are barely lost.

The invention belongs to the field of environmental protection and relates to a method for treating restaurant and kitchen garbage by utilizing maggots of boettcherisca peregrine, which comprises the following steps: adding auxiliary materials after coarse crushing of the restaurant and kitchen garbage, obtaining a compost for the maggots of the boettcherisca peregrine, further inoculating larvaeof the maggots of the boettcherisca peregrine on the surface of the compost for the maggots of the boettcherisca peregrine, obtaining a feeding compost for the maggots of the boettcherisca peregrine and treating and clearing the garbage. A polypide collecting container is connected at the outside of a stacking region of the compost for the maggots of the boettcherisca peregrine before aging of the larvae of the boettcherisca peregrine, and dry rice bran powder is arranged in the polypide collecting container for guiding the collection of the aged maggots of the boettcherisca peregrine. The residual treated compost for the maggots of the boettcherisca peregrine is prepared into a biological organic fertilizer after drying, the method has strong treatment capacity and faster treatment speedin comparison with an ordinary manure, and the treatment cycle is 4-5 days; the capacity of the food garbage is reduced by 10-30% after the treatment, and the peculiar smell of the food garbage is reduced; and the method can obtain the high-quality biological organic fertilizer and high-quality insect bioprotein while treating the garbage.

The invention provides a living Chinese caterpillar fungi artificially cultivated by using larvae as hosts, which comprise insect larvae and one or two about two centimeter living Chinese caterpillar fungus fruiting bodies grown on the head of each the larva. The cultivating process comprises the steps of: invasion of the Chinese caterpillar fungi into the living insect larvae, in vivo growth of the hyphae in the living insect larvae, inactivation of the larvae, growth of the Chinese caterpillar fungus fruiting bodies on the heads and the like; and in the whole process, not only the insect larvae and the hyphae are grown in the polypide, but also the Chinese caterpillar fungus fruiting bodies are grown on the heads, the obtained Chinese caterpillar fungi have the same appearance and almost the same nutrient content as the wild Chinese caterpillar fungi, so that the Chinese caterpillar fungi obtained by the method are the real living Chinese caterpillar fungi obtained by artificial cultivation.

The invention belongs to the field of environmental protection, relating to a method for processing utensil rubbishes by Chrysomya megacephala maggot. The method comprises the following steps: after the utensil rubbishes are coarsely crushed, auxiliary materials are added to obtain a Chrysomya megacephala maggot culture material; Chrysomya megacephala ovum is accessed on the surface of the Chrysomya megacephala maggot culture material; the larvae incubated by the Chrysomya megacephala maggot eat and decompose the culture material to process and clear the utensil rubbishes. Before the Chrysomya megacephala larvae are aged, the outer part of Chrysomya megacephala maggot culture material stacking area is connected with a polypide collection container in which dried rice bran powder is arranged for guiding and collecting aged Chrysomya megacephala maggot. Residual processed Chrysomya megacephala maggot culture material is dried and processed into an organic fertilizer. The method of the invention has strong processing capability and higher processing speed than common compost; the processing period is 4-5 days; after processed, the capacity of the utensil rubbishes is reduced by 10-30%, and a peculiar smell is reduced; high-quality organic fertilizer and high-quality insect biologic protein are obtained while processing the utensil rubbishes.

The invention relates to a cultivating method of zinc-rich yellow mealworm, belonging to the technical field of cultivation. The method comprises the steps of cultivating larvae and cultivating enriched zinc, specifically comprising the steps of: cultivating the yellow mealworm to be middle-age stage and old-age stage according to a routine method; transferring the middle-aged yellow mealworm andthe old-aged yellow mealworm into a special cultivating box; cultivating with wheat bran; daily cultivating with 0.6-5%-2.0% of zinc sulfate water solution with a water drinking device in a special cultivating box, wherein the usage amount is 600ml/kg polypide; and collecting and using after the 12-15 days of continues cultivation. The method can improve the content of microelement zinc in the yellow mealworm by 3-4 times to reach about 400mg/kg.

The invention relates to a method for breeding a large number of microplitis bicoloratus for controlling noctuidae injurious insects, belonging to the field of insect management. Beet armyworm or prodenia litura larvas between the end of second instar and the beginning of third instar are used as intermediate hosts, and microplitis bicoloratus is used as parasitic wasps for wasp inoculation, wherein the quantity ratio of the parasitic wasps to the hosts is 1:5-10. The hosts are bred under the conditions that temperature is 25-27 DEG C, RH (Relative Humidity) is 60-70%, photoperiod L:D is 2:12 and light intensity is 500 LUX. The parasitic wasps come out of polypide for cocooning, pupation and eclosion to obtain the microplitis bicoloratus. The invention realizes the efficient breeding of a large number of high-quality graparasitic wasps, namely microplitis bicoloratus which can be applied to the control of prodenia litura; the invention provides a solid basis for applying the microplitis bicoloratus to the control of the noctuidae injurious insects in large lands. The method is not limited by seasons, ensures the long-time abundant supply source of parasitic wasps and is beneficial to the long-time efficient control of noctuidae.

The invention relates to an American cockroach-honey refined flavor cream and a preparation method thereof. The American cockroach-honey refined flavor cream consists of the following compositions in percentage by weight: 55 to 60 percent of American cockroaches, 35 to 40 percent of honey and 0 to 10 percent of auxiliary materials, wherein the auxiliary materials are pollen pini, sweet osmanthus, pale butterflybush flower, rose or Saruma henryi. The preparation process comprises the following steps: using 70 DEG C hot water to soak and kill live insects of the American cockroaches; baking and drying and sterilizing polypide as soon as possible, and crushing the polypide into thick paste; and mixing and stirring the treated American cockroaches, the auxiliary materials which are sterilized and crushed into powder, and the honey which is subjected to hot smelt in a water bath, in a mixer to obtain the finished product. The prepared product not only maintains the special health-care, nourishing and beautifying efficacies of the American cockroaches, but also adds the nutrition of the honey and the auxiliary materials, and forms high-grade health food with special flavor. The processing technique is simple, convenient and easy.

The invention discloses a prawn white spot comprehensive virus capsule membrane protein vp28 and genetic engineering strain of carp growth hormone GH fused protein and constructing method and appliance, which comprises the following steps: extracting total RNA from glandula pituitaria of prawn; reverse- transcripting; getting GH gene; extracting WSSV from ill prawn polypide; preparing vp28 gene through PCR method; constructing shuttle plasmid pPIC6alphac-GH-vp28 with growth hormone GH gene and WSSV vp28 gene; cutting pPIC6alphac-GH-vp28 with enzyme; making to linearization; inverting yeast X33 competence cell; inducing with methanol; expressing genetic engineering fused protein GH+VP28 protein in pichia. This protein can either accelerate growth of prawn, or increases immunity and disease resistance for WSSV.

The invention relates to the field of analytical chemistry, specifically to an infrared spectroscopic analysis and identification method for the polypide of Cordyceps sinensis. The objective of the invention is to overcome the technical problem that whether the polypide of Cordyceps sinensis is true or false is difficult to determine at present. Thus, the invention provides the method for rapid and accurate discrimination of the polypide of Cordyceps sinensis. The method comprises the following steps: a, drafting the standard spectrum of the polypide of Cordyceps sinensis; b, acquiring necessary infrared spectroscopic characteristic peaks and common infrared spectroscopic characteristic peaks of the polypide of Cordyceps sinensis; and c, comparing infrared spectroscopic characteristic peaks and infrared spectroscopic similarity of a detected sample of the polypide of Cordyceps sinensis. The invention provides the novel method for rapid and accurate discrimination of the polypide of Cordyceps sinensis.

The invention belongs to the technical field of solid waste resource treatment, biomass resource treatment and ecological cycle economy, and discloses a family-type kitchen garbage biological circulation processing system and method. Kitchen garbage on that day is collected, chopped and processed; the chopped kitchen garbage and wheat bran are mixed according to the ratio being 1:1 to prepare flour weevil larvae feed, and the feed is put into a flour weevil larvae breeding box; and flour weevil fecula sand is collected to make organic fertilizer or potted plant food. According to the family type kitchen garbage biological circulation processing system and method, the kitchen garbage produced each day in a family is collected from the source to be converted into high-worth fecula sand and polypide albumen through feeding of flour weevil, the fecula sand can serve as the fertilizer to be applied to a balcony vegetable garden, and the garbage goes back to a kitchen and a dining-table through vegetable picking. The complex feeding habits of the flour weevil larval are used, and the kitchen garbage with the complex ingredient are eaten; meanwhile, insects do not urinate due to the special intestinal tract (malpighian tubule) condition, the problem of environmental pollution of the kitchen garbage in the flour weevil fecula biotransformation process is effectively solved.

The invention discloses an attractant of tomicus yunnanensis. The attractant comprises one or more of the following components: S-(-)-alpha-pinene, 1R-(+)-alpha-pinene, myrcene, (+)-camphene, (-)-camphene, alpha-terpinene, (+)-3-carene, (1s)-(-)-beta-pinene, (-)-limonene, (+)-limonene, (-)-Myrteneol, (s)-cis-verbenol, (-)-trans-verbenol and Cuparene. According to the attractant disclosed by the invention, the attractant containing a host and polypide volatile matter is utilized for attract tomicus yunnanensis massively, so that the tomicus yunnanensis is gathered in a certain device and then is killed; the attractant has the advantages of low cost, high efficiency, complete environmental friendliness, safety to people and livestock, incapability of generating drug resistance to insects and the like, so that the traditional passive chemical prevention and treatment can be replaced by active biological prevention and treatment; meanwhile, the aims of monitoring and forecasting the forestry insect pests can be achieved according to the amount of tomicus yunnanensis in the attractant.

A novel technique for artificially culturing cordyceps without worm includes such steps as using hybridized cordyceps fungus to infect the solid rice culture medium or semi-solid agar-glucose culturemedium, artificial culturing at 20-28 deg.C in germination stage, variable-temp culturing (26-28 deg.C on day and 4-6 deg.C at night) in growth stage for 5-7 days, growing grass, and diasporing. Its advantages are no need of worm, short period (40 days), high effect and low cost.

The invention discloses an in-vitro culture method of balantidium coli. The in-vitro culture method of balantidium coli comprises the following steps of (1) polypide inoculation: inoculating pollutant containing balantidium coli in an RSS culture medium and culturing for 48 h at a temperature of 28 DEG C; (2) preparation of the culture medium: preparing the culture medium according to 15-25% by volume of new-born calf serum and 75-85% by volume of a 199 culture medium with a pH value of 6.0-8.0, adding 20-40 mg of starch in every 3 mL of the culture medium; and (3) polypide culture: (a) adding 10,000 U/mL of penicillin and streptomycin in the culture medium formed by the step (2), (b) inoculating a balantidium coli suspension in the culture medium formed by the step (a), culturing for 96-108 h at the temperature of 28 DEG C to obtain balantidium coli having the highest population density being 42.75-74.5 times of an inoculation density. The in-vitro culture method of balantidium coli has the advantages of simple preparation method, convenient operations, relatively good stability and high population density.

The invention discloses an establishment method of an animal model in mice infected with sparganosis mansoni, comprising the following steps: cleaning and soaking polypide taken out from the body of the animal naturally infected with sparganum mansoni with sterilized normal saline; injecting the polypide into the gullets of the healthy mice by the method for pouring belly for inoculation; after raising the inoculated mice for a period, transplanting the inoculated mice by the method for pouring belly, carrying out standard raising on the transplanted mice and carrying out one-time new rejuvenation transplantation and collection on the infected mice which are raised for a period within 3-12 months; and establishing the stable animal model in mice infected with sparganum mansoni. The method specifies the collection method of the sparganum mansoni species resources and the living body conservation operation technology, provides the stable disease animal model for the research on immunity characteristics, immunopathological injury, diagnosis and treatment of sparganosis mansoni and provides the standard species resources with stable biological characteristics for the scientific research, teaching, quarantine and diagnostic antigen production units.

The invention relates to a roach bait, and belongs to the technical field of pesticides. The roach bait consists of the following raw materials in percentage by weight: 0.2 to 0.6 percent of ivermectin, 79 to 85 percent of gelatinized flour, 14 to 20 percent of sugar, 0.2 to 0.5 percent of gelatinized sesame powder, 0.2 to 0.5 percent of milk powder and 0.02 to 0.2 percent of fumaric acid. The ivermectin in the roach bait is taken as an effective pesticidal composition, and mainly has the function of increasing the release of inhibitory neurotransmitter gamma aminobutyric acid (GABA) of polypide so as to prevent the transmission of neural signals, make muscle cells lose contractility and make the polypide dead. However, the peripheral neurotransmitter of mammals is acetylcholine and cannot be influenced by the ivermectin, and the ivermectin is difficult to permeate through hemato encephalic barrier, so that the ivermectin has high safety coefficient. The gelatinized four, the sugar, the gelatinized sesame powder and the milk powder form a phagostimulant; and the fumaric acid is taken as an antiseptic so as to improve the effective period of products. The roach bait has the advantages of good palatability, strong attractive effect, high efficiency and low toxicity, convenient application, low dosage, environmental protection, and the like.

The invention discloses a method for identifying Anguina agrostis by applying a real-time fluorescent PCR technology. The specific DNA sequence of the Anguina agrostis is utilized; a bioinformatics method is used to design a specific primer and a specific probe; a 5' end of the probe is labeled with a FAM; a 3' end of the probe is labeled with TAMRA; a target gene of the Anguina agrostis is amplified by applying a fluorescent PCR method; and whether the Anguina agrostis is contained in a sample is identified through the change of a fluorescent signal of the probe during the reaction. The method is not affected by larva instar and the quantity of polypides, is quicker and more accurate compared with morphology and morphometry identification methods, has simple operation, can be standardized, solves the problem of a standard method for quickly identifying the Anguina agrostis, greatly saves detection time, accelerates the acceptance of grass seeds at ports, promotes the import and export trade, and saves the cost generated by the long-term storage of goods in a goods yard.

The invention discloses a method for artificially breeding Sclerodermus pupariae by using local alternate hosts. The method at least comprises the following steps: (1) collecting the alternate hosts; (2) feeding and storing the collected alternate hosts; (3) preserving Sclerodermus pupariae germplasm; (4) inoculating the Sclerodermus pupariae germplasm; and (5) feeding natural enemy inoculated polypides. According to the method, the Sclerodermus pupariae can be artificially bred and fed locally in Yili, Xinjiang, unnecessary loss of parasitic bees resulted from long-distance carriage is reduced, the vitality is improved, and the cost is reduced.

The invention discloses rabbit anti-Hematodinium sp serum, which is rabbit antiserum which is obtained by first using Hematodinium sp polypide-containing body fluid of an infected crab as an antigen, then processing the antigen to obtain an immunizing antigen which is a Hematodinium sp polypide suspension and finally vaccinating a white rabbit. The invention also discloses application of the rabbit antiserum to a method for indirect fluorescent antibody detection, which is implemented by dripping the rabbit antiserum into a sample to be detected for primary antibody incubation, dripping isosulfocyanic acid fluorescent labeled sheep and rabbit antibodies for secondary antibody incubation and performing microscopic examination after mounting processing. The rabbit antiserum for detecting Hematodinium sp of the invention has strong specificity, high sensitivity and short detection time, and can be used for diagnosing the potential infection and early infection with the Hematodinium sp. The detection method of the invention is simple in operation and performs qualitative and quantitative detection on whether sea food shellfish is infected with the Hematodinium sp.

The invention discloses an improved Grocott silver hexosamine staining method and applications thereof. The staining method comprises the steps of preparing paraffin-embedded sections, staining, carrying out dehydrated hyalinizing, and carrying out section sealing, wherein in the step of preparing paraffin-embedded sections, a sliced paraffin section is adhered to a glass slide subjected to APES treatment. The method overcomes the defect that section shedding easily occurs in the process of staining, especially for frankliniella occidentalis with a small somatotype, the non-section-shedding rate is over 70%. In addition, the internal tissues of polypides in sections obtained according to the staining method disclosed by the invention are red, blastospores in polypides are black, therefore, the polypides are clearly visible, and the staining effect is excellent.