79 results about "Polypide" patented technology

The invention belongs to the field of environmental protection, relating to a method for processing food waste by using black soldier fly larvae and comprising the following steps: coarse crushing the food waste and then adding auxiliary materials to obtain culture materials for the black soldier fly larvae; and grafting black soldier fly spawn on the surface of the culture materials for the black soldier fly larvae so that the larvae hatched by the black soldier fly spawn acquires the culture materials for the black soldier fly larvae, thereby processing and cleaning the food waste. Before the black soldier fly larvae ages, a polypide collecting container is externally connected to the area for piling up the culture materials for the black soldier fly larvae and is filled with dry rice bran powder so as to guide to collect the aged black soldier fly larvae; and the rest of the processed culture materials for the black solider fly larvae is dried and then prepared into microbial organic fertilizer. The invention has strong processing capability, rapid processing speed compared with the common compost, the processing period is 4-5 days; after being processed, the volume of the food waste is reduced by 10-30%, the peculiar smell of the waste is lightened; and the invention can obtain excellent microbial organic fertilizer and insect bioprotein at the same time.

The invention discloses a preservation method of cordyceps sinensis, which comprises the steps of sequentially cleaning the head part, stroma and polypide of the cordyceps sinensis by use of linear running water; soaking the cleaned cordyceps sinensis in a 0.9% acid sodium chloride for 5-8 minutes, and draining; performing quick cooling treatment on the cordyceps sinensis for 5-12 hours at -75 DEG C; putting the cordyceps sinensis after the quick cooling into a vacuum refrigeration box at (-5)-(-25) DEG C, and starting the refrigeration gas self-control equipment in the refrigeration box, wherein the refrigeration gas self-control equipment controls a gas pump to introduce and / or extract oxygen, nitrogen and carbon dioxide gas into / out of the refrigeration box. By adopting the preservation method disclosed by the invention, the cordyceps sinensis can meet the sanitary requirement of direct eating; after long-time storage, the nutritional ingredients and activity of the cordyceps sinensis are barely lost.

The invention belongs to the field of environmental protection and relates to a method for treating restaurant and kitchen garbage by utilizing maggots of boettcherisca peregrine, which comprises the following steps: adding auxiliary materials after coarse crushing of the restaurant and kitchen garbage, obtaining a compost for the maggots of the boettcherisca peregrine, further inoculating larvaeof the maggots of the boettcherisca peregrine on the surface of the compost for the maggots of the boettcherisca peregrine, obtaining a feeding compost for the maggots of the boettcherisca peregrine and treating and clearing the garbage. A polypide collecting container is connected at the outside of a stacking region of the compost for the maggots of the boettcherisca peregrine before aging of the larvae of the boettcherisca peregrine, and dry rice bran powder is arranged in the polypide collecting container for guiding the collection of the aged maggots of the boettcherisca peregrine. The residual treated compost for the maggots of the boettcherisca peregrine is prepared into a biological organic fertilizer after drying, the method has strong treatment capacity and faster treatment speedin comparison with an ordinary manure, and the treatment cycle is 4-5 days; the capacity of the food garbage is reduced by 10-30% after the treatment, and the peculiar smell of the food garbage is reduced; and the method can obtain the high-quality biological organic fertilizer and high-quality insect bioprotein while treating the garbage.

The invention provides a living Chinese caterpillar fungi artificially cultivated by using larvae as hosts, which comprise insect larvae and one or two about two centimeter living Chinese caterpillar fungus fruiting bodies grown on the head of each the larva. The cultivating process comprises the steps of: invasion of the Chinese caterpillar fungi into the living insect larvae, in vivo growth of the hyphae in the living insect larvae, inactivation of the larvae, growth of the Chinese caterpillar fungus fruiting bodies on the heads and the like; and in the whole process, not only the insect larvae and the hyphae are grown in the polypide, but also the Chinese caterpillar fungus fruiting bodies are grown on the heads, the obtained Chinese caterpillar fungi have the same appearance and almost the same nutrient content as the wild Chinese caterpillar fungi, so that the Chinese caterpillar fungi obtained by the method are the real living Chinese caterpillar fungi obtained by artificial cultivation.

The invention belongs to the field of environmental protection, relating to a method for processing utensil rubbishes by Chrysomya megacephala maggot. The method comprises the following steps: after the utensil rubbishes are coarsely crushed, auxiliary materials are added to obtain a Chrysomya megacephala maggot culture material; Chrysomya megacephala ovum is accessed on the surface of the Chrysomya megacephala maggot culture material; the larvae incubated by the Chrysomya megacephala maggot eat and decompose the culture material to process and clear the utensil rubbishes. Before the Chrysomya megacephala larvae are aged, the outer part of Chrysomya megacephala maggot culture material stacking area is connected with a polypide collection container in which dried rice bran powder is arranged for guiding and collecting aged Chrysomya megacephala maggot. Residual processed Chrysomya megacephala maggot culture material is dried and processed into an organic fertilizer. The method of the invention has strong processing capability and higher processing speed than common compost; the processing period is 4-5 days; after processed, the capacity of the utensil rubbishes is reduced by 10-30%, and a peculiar smell is reduced; high-quality organic fertilizer and high-quality insect biologic protein are obtained while processing the utensil rubbishes.

The invention relates to a method for breeding a large number of microplitis bicoloratus for controlling noctuidae injurious insects, belonging to the field of insect management. Beet armyworm or prodenia litura larvas between the end of second instar and the beginning of third instar are used as intermediate hosts, and microplitis bicoloratus is used as parasitic wasps for wasp inoculation, wherein the quantity ratio of the parasitic wasps to the hosts is 1:5-10. The hosts are bred under the conditions that temperature is 25-27 DEG C, RH (Relative Humidity) is 60-70%, photoperiod L:D is 2:12 and light intensity is 500 LUX. The parasitic wasps come out of polypide for cocooning, pupation and eclosion to obtain the microplitis bicoloratus. The invention realizes the efficient breeding of a large number of high-quality graparasitic wasps, namely microplitis bicoloratus which can be applied to the control of prodenia litura; the invention provides a solid basis for applying the microplitis bicoloratus to the control of the noctuidae injurious insects in large lands. The method is not limited by seasons, ensures the long-time abundant supply source of parasitic wasps and is beneficial to the long-time efficient control of noctuidae.

The invention discloses a production method of white muscardine silkworms. The production method comprises the following steps of: (1) rejuvenescence of pathogens: adopting white muscardine silkworms to infect bombyx mori polypides and collecting fresh beauveria bassiana on the bombyx mori polypides; (2) toxin attacking for the pathogens: preparing the beauveria bassiana into beauveria bassiana liquid, closing doors and windows, uniformly spraying the beauveria bassiana liquid on silkworm bodies with newly-exuviated fifth larva and placing in a sealed space for 1-2 hours; (3) culture of silkworm bodies: opening doors and windows for ventilation, controlling the temperature to 22-26 DEG C and the humidity to 95-98%, and culturing till the silkworm bodies are died; (4) culture of muscardine silkworms: choosing out died silkworm bodies, culturing on a clean silkworm seat paper, controlling the temperature to 24-28 DEG C and the humidity to more than or equal to 80%, culturing till rigidification of the died silkworm bodies, growing white hypha conidia and obtaining semi-finished products of the muscardine silkworms; and (5) preparation of finished products of the white muscardine silkworms: collecting semi-finished products of the white muscardine silkworms, airing and drying to obtain the finished products of the white muscardine silkworms. The production method disclosed by the invention has the advantages that the death time of the silkworm bodies is neat, the rigidification time is short, the rigidification rate is high, the yield of the white muscardine silkworms is high and the quality is stable.

The invention relates to an American cockroach-honey refined flavor cream and a preparation method thereof. The American cockroach-honey refined flavor cream consists of the following compositions in percentage by weight: 55 to 60 percent of American cockroaches, 35 to 40 percent of honey and 0 to 10 percent of auxiliary materials, wherein the auxiliary materials are pollen pini, sweet osmanthus, pale butterflybush flower, rose or Saruma henryi. The preparation process comprises the following steps: using 70 DEG C hot water to soak and kill live insects of the American cockroaches; baking and drying and sterilizing polypide as soon as possible, and crushing the polypide into thick paste; and mixing and stirring the treated American cockroaches, the auxiliary materials which are sterilized and crushed into powder, and the honey which is subjected to hot smelt in a water bath, in a mixer to obtain the finished product. The prepared product not only maintains the special health-care, nourishing and beautifying efficacies of the American cockroaches, but also adds the nutrition of the honey and the auxiliary materials, and forms high-grade health food with special flavor. The processing technique is simple, convenient and easy.

The invention discloses a paecilomyces hepialid mycelium and a preparation method thereof. The mycelium is obtained by cultivating paecilomyces hepialid via a solid culture medium. The preparation method technically includes: dissecting fresh cordyceps sinensis polypide, separating a paecilomyces hepialid culture in the cordyceps sinensis polypide, cultivating and separating hetero-cultures out, preparing the solid culture medium for sterilizing, inoculating 10% by weight of cultivated and rejuvenated liquid culture on the culture medium for fermentation, and cultivating in an aseptic condition at the temperature of 20-22DEG C for 7-10 days to obtain the mycelium. The mycelium prepared by the preparation method is equal to natural cordyceps sinensis in aspects of nutrition and medical values. The paecilomyces hepialid mycelium is characterized in that under an ordinary 300-600-multiplication optical microscope, the mycelium is 16-40 micrometers in visible length and 3-5 micrometers in root width. The mycelium mainly comprises adenosine which cannot be dissolved by water and can be dissolved by ethyl alcohol.

The invention relates to the field of analytical chemistry, specifically to an infrared spectroscopic analysis and identification method for the polypide of Cordyceps sinensis. The objective of the invention is to overcome the technical problem that whether the polypide of Cordyceps sinensis is true or false is difficult to determine at present. Thus, the invention provides the method for rapid and accurate discrimination of the polypide of Cordyceps sinensis. The method comprises the following steps: a, drafting the standard spectrum of the polypide of Cordyceps sinensis; b, acquiring necessary infrared spectroscopic characteristic peaks and common infrared spectroscopic characteristic peaks of the polypide of Cordyceps sinensis; and c, comparing infrared spectroscopic characteristic peaks and infrared spectroscopic similarity of a detected sample of the polypide of Cordyceps sinensis. The invention provides the novel method for rapid and accurate discrimination of the polypide of Cordyceps sinensis.

The invention belongs to the technical field of solid waste resource treatment, biomass resource treatment and ecological cycle economy, and discloses a family-type kitchen garbage biological circulation processing system and method. Kitchen garbage on that day is collected, chopped and processed; the chopped kitchen garbage and wheat bran are mixed according to the ratio being 1:1 to prepare flour weevil larvae feed, and the feed is put into a flour weevil larvae breeding box; and flour weevil fecula sand is collected to make organic fertilizer or potted plant food. According to the family type kitchen garbage biological circulation processing system and method, the kitchen garbage produced each day in a family is collected from the source to be converted into high-worth fecula sand and polypide albumen through feeding of flour weevil, the fecula sand can serve as the fertilizer to be applied to a balcony vegetable garden, and the garbage goes back to a kitchen and a dining-table through vegetable picking. The complex feeding habits of the flour weevil larval are used, and the kitchen garbage with the complex ingredient are eaten; meanwhile, insects do not urinate due to the special intestinal tract (malpighian tubule) condition, the problem of environmental pollution of the kitchen garbage in the flour weevil fecula biotransformation process is effectively solved.

The invention relates to novel cheap high-protein microbial fermentation feed. The high-protein microbial fermentation feed comprises the following components in part by weight: 25 parts of polypide, 420 parts of worm sand, 40 parts of bacterium liquid, 20 parts of composite multivitamin, 340 parts of soy sauce lees and 155 parts of water. The invention has the advantages as follows: the novel cheap high-protein microbial fermentation feed has low cost by using leftovers such as the soy sauce lees and the worm sand as a main matrix; the protein content in the novel cheap high-protein microbial fermentation feed can reach 30-40%; the novel cheap high-protein microbial fermentation feed can be directly fed as an animal diet to substitute 40-60% of complete formula feed, so that the grains are saved and the breeding cost is greatly reduced; and according to the invention, a product fermented by three microorganisms contains higher-content small molecular active substances such as absorbable protein, active peptide and amino acid; and protein and amino acid required in the growing process of livestock and poultry can be effectively supplemented.

The invention discloses an attractant of tomicus yunnanensis. The attractant comprises one or more of the following components: S-(-)-alpha-pinene, 1R-(+)-alpha-pinene, myrcene, (+)-camphene, (-)-camphene, alpha-terpinene, (+)-3-carene, (1s)-(-)-beta-pinene, (-)-limonene, (+)-limonene, (-)-Myrteneol, (s)-cis-verbenol, (-)-trans-verbenol and Cuparene. According to the attractant disclosed by the invention, the attractant containing a host and polypide volatile matter is utilized for attract tomicus yunnanensis massively, so that the tomicus yunnanensis is gathered in a certain device and then is killed; the attractant has the advantages of low cost, high efficiency, complete environmental friendliness, safety to people and livestock, incapability of generating drug resistance to insects and the like, so that the traditional passive chemical prevention and treatment can be replaced by active biological prevention and treatment; meanwhile, the aims of monitoring and forecasting the forestry insect pests can be achieved according to the amount of tomicus yunnanensis in the attractant.

The invention provides an eimeria tenella AN1-like Zinc finger domain-containing protein and an application thereof in inhibiting invasion of coccidium. The eimeria tenella AN1-like Zinc finger domain-containing protein is cloned, expressed and purified. The transcription level of EtAN1-ZnFP in different developmental stages of polypides is detected by fluorescence quantitative PCR (qPCR). The immune protective effect of EtAN1-ZnFP after chickens are infected with coccidium is evaluated by immunoprotection experiments. Research results indicate that the EtAN1-ZnFP is possible to participate ininvasion and growing development of eimeria tenella.

A novel technique for artificially culturing cordyceps without worm includes such steps as using hybridized cordyceps fungus to infect the solid rice culture medium or semi-solid agar-glucose culturemedium, artificial culturing at 20-28 deg.C in germination stage, variable-temp culturing (26-28 deg.C on day and 4-6 deg.C at night) in growth stage for 5-7 days, growing grass, and diasporing. Its advantages are no need of worm, short period (40 days), high effect and low cost.

The invention discloses an in-vitro culture method of balantidium coli. The in-vitro culture method of balantidium coli comprises the following steps of (1) polypide inoculation: inoculating pollutant containing balantidium coli in an RSS culture medium and culturing for 48 h at a temperature of 28 DEG C; (2) preparation of the culture medium: preparing the culture medium according to 15-25% by volume of new-born calf serum and 75-85% by volume of a 199 culture medium with a pH value of 6.0-8.0, adding 20-40 mg of starch in every 3 mL of the culture medium; and (3) polypide culture: (a) adding 10,000 U / mL of penicillin and streptomycin in the culture medium formed by the step (2), (b) inoculating a balantidium coli suspension in the culture medium formed by the step (a), culturing for 96-108 h at the temperature of 28 DEG C to obtain balantidium coli having the highest population density being 42.75-74.5 times of an inoculation density. The in-vitro culture method of balantidium coli has the advantages of simple preparation method, convenient operations, relatively good stability and high population density.

The invention discloses an establishment method of an animal model in mice infected with sparganosis mansoni, comprising the following steps: cleaning and soaking polypide taken out from the body of the animal naturally infected with sparganum mansoni with sterilized normal saline; injecting the polypide into the gullets of the healthy mice by the method for pouring belly for inoculation; after raising the inoculated mice for a period, transplanting the inoculated mice by the method for pouring belly, carrying out standard raising on the transplanted mice and carrying out one-time new rejuvenation transplantation and collection on the infected mice which are raised for a period within 3-12 months; and establishing the stable animal model in mice infected with sparganum mansoni. The method specifies the collection method of the sparganum mansoni species resources and the living body conservation operation technology, provides the stable disease animal model for the research on immunity characteristics, immunopathological injury, diagnosis and treatment of sparganosis mansoni and provides the standard species resources with stable biological characteristics for the scientific research, teaching, quarantine and diagnostic antigen production units.

The invention relates to an entomogenous fungus-Verticillium lecanii, which is particularly effective in preventing and treating familiar dipterous fly insects. A wild strain is separated from the polypide of a Sarcophga fuscicauda imago which is naturally infected with the entomogenous fungus, cultured on a potato dextrose agar culture medium (PDA), and inoculated into the Sarcophga fuscicauda imago for rejuvenescence and acquisition of a strain; and after the strain is subjected to monomycelial separation, a purified strain, namely Verticillium lecanii KMZW-1(CGMCC No.2728), is obtained. The strain has good growth conditions at a temperature of between 28 and 29 DEG C and with a relative humidity of more than 90 percent, and has large spore yield and high spore germination rate; and spore suspension of the strain has better pathogenicity on imagoes of Sarcophga fuscicauda, lucilia sericata, houseflies, Piophila casei and drosophila.

The invention relates to a roach bait, and belongs to the technical field of pesticides. The roach bait consists of the following raw materials in percentage by weight: 0.2 to 0.6 percent of ivermectin, 79 to 85 percent of gelatinized flour, 14 to 20 percent of sugar, 0.2 to 0.5 percent of gelatinized sesame powder, 0.2 to 0.5 percent of milk powder and 0.02 to 0.2 percent of fumaric acid. The ivermectin in the roach bait is taken as an effective pesticidal composition, and mainly has the function of increasing the release of inhibitory neurotransmitter gamma aminobutyric acid (GABA) of polypide so as to prevent the transmission of neural signals, make muscle cells lose contractility and make the polypide dead. However, the peripheral neurotransmitter of mammals is acetylcholine and cannot be influenced by the ivermectin, and the ivermectin is difficult to permeate through hemato encephalic barrier, so that the ivermectin has high safety coefficient. The gelatinized four, the sugar, the gelatinized sesame powder and the milk powder form a phagostimulant; and the fumaric acid is taken as an antiseptic so as to improve the effective period of products. The roach bait has the advantages of good palatability, strong attractive effect, high efficiency and low toxicity, convenient application, low dosage, environmental protection, and the like.

The invention discloses a method for identifying Anguina agrostis by applying a real-time fluorescent PCR technology. The specific DNA sequence of the Anguina agrostis is utilized; a bioinformatics method is used to design a specific primer and a specific probe; a 5' end of the probe is labeled with a FAM; a 3' end of the probe is labeled with TAMRA; a target gene of the Anguina agrostis is amplified by applying a fluorescent PCR method; and whether the Anguina agrostis is contained in a sample is identified through the change of a fluorescent signal of the probe during the reaction. The method is not affected by larva instar and the quantity of polypides, is quicker and more accurate compared with morphology and morphometry identification methods, has simple operation, can be standardized, solves the problem of a standard method for quickly identifying the Anguina agrostis, greatly saves detection time, accelerates the acceptance of grass seeds at ports, promotes the import and export trade, and saves the cost generated by the long-term storage of goods in a goods yard.

The invention discloses an anti-Pf332-DBL area monoclonal antibody capable of restraining the invasion of plasmodiumfalciparum, which can be specifically combined with the DBL region functional polypeptide of plasmodiumfalciparum Pf332 membrane protein, and the polypeptide has the amino acid sequence shown as SEQ ID No.1 or 2. The anti-Pf332-DBL area monoclonal antibody is prepared by fusing splenocyte and Sp2 / 0 cells through a DBL recombination protein immunity mouse with a HIS mark, primarily selecting by using a DBL recombination protein with a GST mark, and carrying out positive selecting by using a functional polypeptide. The anti-Pf332-DBL area monoclonal antibody has stronger function for restraining the invasion of polypide, and the function is stronger than that of an MP antibody purified from Mlli human serum infected by plasmodiumfalciparum. A monoclonal antibody which can not be specifically combined with the functional polypeptide has weaker function for restraining the invasion of polypide or has no function for restraining the invasion of polypide. The DBL region functional polypeptide can be applied to the research and development of polypeptide medicaments for treating malaria and the preparation of preventing active polypeptide vaccine.

The invention discloses a method for artificially breeding Sclerodermus pupariae by using local alternate hosts. The method at least comprises the following steps: (1) collecting the alternate hosts; (2) feeding and storing the collected alternate hosts; (3) preserving Sclerodermus pupariae germplasm; (4) inoculating the Sclerodermus pupariae germplasm; and (5) feeding natural enemy inoculated polypides. According to the method, the Sclerodermus pupariae can be artificially bred and fed locally in Yili, Xinjiang, unnecessary loss of parasitic bees resulted from long-distance carriage is reduced, the vitality is improved, and the cost is reduced.

The invention discloses rabbit anti-Hematodinium sp serum, which is rabbit antiserum which is obtained by first using Hematodinium sp polypide-containing body fluid of an infected crab as an antigen, then processing the antigen to obtain an immunizing antigen which is a Hematodinium sp polypide suspension and finally vaccinating a white rabbit. The invention also discloses application of the rabbit antiserum to a method for indirect fluorescent antibody detection, which is implemented by dripping the rabbit antiserum into a sample to be detected for primary antibody incubation, dripping isosulfocyanic acid fluorescent labeled sheep and rabbit antibodies for secondary antibody incubation and performing microscopic examination after mounting processing. The rabbit antiserum for detecting Hematodinium sp of the invention has strong specificity, high sensitivity and short detection time, and can be used for diagnosing the potential infection and early infection with the Hematodinium sp. The detection method of the invention is simple in operation and performs qualitative and quantitative detection on whether sea food shellfish is infected with the Hematodinium sp.

The invention discloses an improved Grocott silver hexosamine staining method and applications thereof. The staining method comprises the steps of preparing paraffin-embedded sections, staining, carrying out dehydrated hyalinizing, and carrying out section sealing, wherein in the step of preparing paraffin-embedded sections, a sliced paraffin section is adhered to a glass slide subjected to APES treatment. The method overcomes the defect that section shedding easily occurs in the process of staining, especially for frankliniella occidentalis with a small somatotype, the non-section-shedding rate is over 70%. In addition, the internal tissues of polypides in sections obtained according to the staining method disclosed by the invention are red, blastospores in polypides are black, therefore, the polypides are clearly visible, and the staining effect is excellent.