3 results about "Polypide" patented technology

The invention relates to an in-vitro culture method and application of anerennial crabs, and belongs to the technical field of aquaculture. The invention provides a culture medium. The culture medium comprises hemolymph of healthy animals and sterile seawater, the volume ratio of the hemolymph of the healthy animal to the sterile seawater is (1-5): (5-9). According to the in-vitro culture method of the crabbidae, the survival time of the crabbidae inoculated into the culture medium for one time is not less than 1 month under the culture system, and when the crabbidae body reaches the optimal state, the culture is transferred to the condition of 2-8 DEG C, so that the survival time of the crabbidae body can be prolonged to be not less than 2 months, and the survival time of the crabbidae body can be prolonged to be not less than 2 months. If the strains are continuously transferred to a fresh culture medium, long-term stable passage of the polypide can be realized, the in-vitro culture system can effectively guarantee supply of the polypide in non-epidemic seasons, and a key technical support is provided for accelerating research and development of quasi-amarerenosis drugs and realizing effective prevention and control of the quasi-amarerenosis.

This invention relates to the field of aquaculture technology, and in particular to an immune enhancer for juvenile large yellow croaker (Culter alburnus), its preparation method, and its application. The immune enhancer provided by this invention, through the compounding of specific components in a specific ratio, achieves the synergistic effect of polypeptides, immunopolysaccharides, antioxidants, and nutritional factors, while also considering rapid absorption, immune regulation, and high-temperature environmental stability. It is suitable for the immune enhancement needs of large yellow croaker juveniles under high-temperature seawater conditions of 29-31 °C, and can enhance the non-specific and specific immunity of juveniles under high-temperature seawater conditions, improving disease resistance and survival rate.

The invention provides an anisakis quarantine method, which belongs to the technical field of parasite quarantine, and comprises the following steps: S1, sample treatment: placing fish tissues in a stationary liquid for fixation; s2, extracting total DNA: after soaking in a TE buffer solution, extracting genome DNA of the polypide, and storing at-15 to-25 DEG C; s3, designing primers, namely designing and synthesizing an upstream primer Anisakis1 and a downstream primer Anisakis2; s4, carrying out PCR (Polymerase Chain Reaction) amplification, namely carrying out PCR amplification on the polypide genome DNA by using the primer; s5, result judgment: performing result judgment on the target amplified fragment through electrophoresis detection; s6, insect species identification: carrying out real-time PCR detection on the positive sample; and S7, judging a result: judging according to a Ct value and an amplification curve. The anisakis quarantine method provided by the invention not only can determine whether the anisakis belongs to anisakis, but also can determine which anisakis specifically carried.