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Technique for artificial breeding Chinese caterpillar fungus with out polypide

A technology of artificial cultivation and new technology, applied in the field of bioengineering of artificially growing Cordyceps sinensis, can solve the problems of shortage of natural Cordyceps sinensis resources, difficult promotion, long growth cycle, etc., and achieve the effects of low cost, simple cultivation process and short growth cycle.

Inactive Publication Date: 2003-10-01
李健
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AI Technical Summary

Problems solved by technology

Although the above-mentioned technology can breed Cordyceps sinensis with a complete shape to solve the shortage of resources and the contradiction between supply and demand of natural Cordyceps sinensis, it is difficult to popularize in areas with fewer host insect sources or generation host insect sources.
In addition, its growth cycle is longer, a total of 280 days

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] 1. Cultivate hybrid Cordyceps sinensis fungus:

[0017] a. Collect fresh Cordyceps sinensis, isolate and obtain excellent Cordyceps strains, and its bacterial numbers are Paecilomyces cordyceps and Cephalosporium cordyceps;

[0018] b. Screening, training and cultivating two Cordyceps strains, Paecilomyces cordyceps and Cephalosporium cordyceps, to change their physiological properties;

[0019] c. two Cordyceps strains of Paecilomyces cordyceps and Cephalosporium cordyceps are carried out protoplast fusion is hybridization;

[0020] d. Using the healthy cell propagation method, introduce healthy cells into the cyclic breeding process, pick out the bad and keep the good, and cultivate pure and strong Cordyceps mycelium for future use.

[0021] 2. Preparation of rice solid medium and agar, glucose semi-solid medium:

[0022] Weigh the components of the rice solid medium according to the following proportions: 1000g of rice, 2g or 3g of silkworm chrysalis powder; the pr...

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Abstract

A novel technique for artificially culturing cordyceps without worm includes such steps as using hybridized cordyceps fungus to infect the solid rice culture medium or semi-solid agar-glucose culturemedium, artificial culturing at 20-28 deg.C in germination stage, variable-temp culturing (26-28 deg.C on day and 4-6 deg.C at night) in growth stage for 5-7 days, growing grass, and diasporing. Its advantages are no need of worm, short period (40 days), high effect and low cost.

Description

technical field [0001] The invention relates to a new technology for artificially cultivating insect-free Cordyceps, belonging to the bioengineering technical field of using hybrid Cordyceps fungus to infect rice solid medium or agar and glucose semi-solid medium to artificially cultivate and grow Cordyceps. Background technique [0002] "New technology for cultivating Cordyceps sinensis with insects" (application number: 01141386.7) applied by the applicant, its technical points are: (1) at first cultivate the pure and strong strains of Cordyceps sinensis: collect wild fresh Cordyceps sinensis, and obtain the strains of Cordyceps sinensis with tissue separation method , use the healthy cell propagation method to inoculate the strains and save them for later use; cultivate inclined-plane mother species and liquid bacteria for infecting worms; (2) select hosts or generation hosts that are susceptible to bacteria, and spray the bacteria liquid to infect the worms (3) Simulate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N15/04
Inventor 李健
Owner 李健
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