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Rapid artificial cultivation technology for cordyceps sinensis

A technology for artificial cultivation of Cordyceps sinensis, which is applied in the field of bioengineering for artificially growing Cordyceps sinensis, can solve the problems of shortage of natural Cordyceps sinensis resources, difficulty in promotion, long growth cycle and the like, and achieves the effects of low cost, simple cultivation process and short growth cycle.

Inactive Publication Date: 2014-09-10
王云飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned technology can breed Cordyceps sinensis with a complete shape to solve the shortage of resources and the contradiction between supply and demand of natural Cordyceps sinensis, it is difficult to popularize in areas with fewer host insect sources or generation host insect sources.
In addition, its growth cycle is longer, a total of 260 days

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] 1. Cultivate hybrid Cordyceps fungus: a. Collect fresh Cordyceps and isolate excellent Cordyceps strains, whose bacterial numbers are Paecilomyces and Cephalosporium Cordyceps; Training and cultivating to change the physiological performance; c. Carrying out protoplast fusion and hybridization of two Cordyceps Paecilomyces and Cephalosporium cordyceps strains; d. Adopting the healthy cell propagation method to introduce healthy cells into the circular breeding process, Pick out the bad and keep the good, and cultivate pure and strong Cordyceps mycelium for future use.

[0011] 2. Preparation of rice solid medium and agar, glucose semi-solid medium:

[0012] Weigh the components of the rice solid medium according to the following proportions: 1000g of rice, 2g or 3g of silkworm chrysalis powder; the preparation method of the rice solid medium is as follows: cook the rice, drain the water, mix in the silkworm chrysalis powder, and then pack In the container, the thicknes...

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Abstract

The invention discloses a rapid artificial cultivation technology for cordyceps sinensis. The technology includes that hybrid cordyceps sinensis fungi are cultured, that is, (1) fresh cordyceps sinensis is collected and separated to obtain good cordyceps sinensis bacterial strains which include cordyceps sinensis paecilomyces varioti and cephalosporium sinensis; (2) the two cordyceps sinensis bacterial strains of the cordyceps sinensis paecilomyces varioti and the cephalosporium sinensis are screened, acclimated and cultured so that physiological properties of the two strains are changed; (3) the two cordyceps sinensis bacterial strains of the cordyceps sinensis paecilomyces varioti and the cephalosporium sinensis are subjected to protoplast fusion, that is, hybridization; (4) healthy cells are guided into a circulation breeding process by a healthy cell reproduction method, poor cells are removed and good cells are reserved, and pure and strong cordyceps sinensis mycelia are obtained by culturing for standby application. According to the rapid artificial cultivation technology for the cordyceps sinensis, the technology gets rid of a pest source, and is convenient to popularize in an area with few pests and short in growth cycle of the cordyceps sinensis, the cordyceps sinensis can be harvested after 40 days' culturing from inoculation and spore germination to cordyceps sinensis ripening, the culturing process is simple, costs are low, and the benefits are high.

Description

technical field [0001] The invention relates to a rapid cultivation technique of artificially cultivated Cordyceps sinensis, belonging to the technical field of bioengineering for artificially cultivating and growing Cordyceps sinensis by using a hybrid Cordyceps sinensis fungus to infect rice solid medium or agar and glucose semi-solid medium. Background technique [0002] At present, the technical points of the existing Cordyceps sinensis on the market are: 1. First, cultivate the pure and strong strains of Cordyceps sinensis: collect wild fresh Cordyceps sinensis, obtain the strains of Cordyceps sinensis with tissue separation method, adopt the healthy cell propagation method to expand the strains and save them for future use ; Cultivate slanted mother species and liquid bacteria for infecting worms; 2. Select hosts or generational hosts that are susceptible to bacteria, and spray bacteria liquid to infect worms; 3. Simulate wild Cordyceps in the stage of germination and g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G3/00
Inventor 王云飞
Owner 王云飞
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