357 results about "Protoplast" patented technology

The invention discloses a tea tree CsU3 gene promoter and application thereof, and belongs to the technical field of plant biotechnology and gene engineering. Tea tree RNA polymerase III type promoters-tea tree endogenous U3 gene promoters CsU3a, CsU3b, CsU3c and CsU3d are successfully cloned for the first time, a tea tree U3 gene promoter activity detection carrier is successfully constructed, the transcriptional activities of the four CsU3 promoters are compared through instantaneous tobacco leaf conversion, GUS dyeing and GUS enzyme activity determination, and the CsU3 gene promoter activity detection carrier can be used for detecting the activity of the tea tree U3 gene promoter. CsU3a and CsU6b promoters with strong transcriptional activity are selected to be used for constructing a tea tree gene editing carrier, the feasibility of the promoter applied to a tea tree CRISPR / Cas9 gene editing system is verified in a tea tree protoplast, and CRISPR / Cas9 mediated tea tree genome targeted editing is realized.

The invention discloses application of threonine dehydratase in regulation and control of growth of Didymeria segeticola and application of threonine dehydratase serving as a bactericide target in regulation and control of growth of Didymeria segeticola. The threonine dehydratase gene Td disclosed by the invention is derived from Didymeria segeticola, and a knockout mutant is obtained by constructing a knockout gene segment of the Td gene and introducing the knockout gene segment into a D.segeticola protoplast. Experiments find that the Td gene plays a role in growth of D.segeticola, in-vivo pyruvic acid content, in-vivo ATP (adenosine triphosphate) content and sensitivity to wuyiencin. Molecular docking analysis, molecular dynamics simulation and trace thermophoresis experiments prove that the threonine dehydratase can be used as a bactericide target, and the bactericide wuyiencin is obtained through screening. The threonine dehydratase disclosed by the invention can be used as a bactericide target and a key protein of a disease pathogenic mechanism, and has a wide application prospect in prevention and treatment of plant pathogenic fungal diseases.

The invention relates to the field of microbial genetic transformation, and discloses a preparation method of Ilyonectria fungus protoplast and a construction method of a genetic transformation system.The preparation method comprises the following steps that S11, Ilyonectria fungus hyphae are prepared, Ilyonectria fungi are cultured on a PDA solid culture medium for 14 d, then the Ilyonectria fungi are inoculated to a CMC liquid culture medium to be subjected to shake-flask culture for 4 d and filtered, filtrate is centrifuged for 10 min at the speed of 10000 rpm, and spores are collected; diluting the concentration of spores to 107 pieces / mL, inoculating the spores into a YEPD liquid culture medium, carrying out shake-flask culture for 2 days, and filtering and collecting hyphae; and S12, preparing the Ilyonectria fungus protoplast, namely adding the hypha prepared in the step S11 into the enzymatic hydrolysate, performing enzymolysis in a shaker at the temperature of 30 DEG C at the rotating speed of 150-200rpm for 3-5 hours, filtering, centrifuging filtrate at the temperature of 4 DEG C, discarding supernate, adding 0.7 M NaCl for centrifuging, and discarding the supernate, thereby obtaining the Ilyonectria fungus protoplast. The genetic transformation constructed by the invention can be used for exploring the specific action and mechanism of a specific pathogenic gene in the pathogenic process of Ilyonectria fungi through gene knockout or overexpression experiments.

The invention discloses a polygonatum cyrtonema transcription factor gene PcWRKY1 as well as an encoding protein and application thereof. According to the application, a WRKY transcription factor PcWRKY1 which is obviously inhibited by high-temperature stress is screened, the transcription level of the PcWRKY1 transcription factor gene responding to the high-temperature stress is further determined, a tissue expression mode and subcellular sites of the PcWRKY1 transcription factor gene are analyzed, and finally, the regulation effect of the PcWRKY1 transcription factor gene on the high-temperature tolerance of the polygonatum cyrtonema protoplast is determined. The PcWRKY1 transcription factor gene is located in a cell nucleus, codes a protein containing 519 amino acids and is expressed in roots, stems, leaves, tubers and seeds of polygonatum cyrtonema, the expression quantity in the seeds is the highest, and the PcWRKY1 transcription factor gene can inhibit the increase of MDA content and promote the improvement of CAT enzyme activity under high temperature stress. Further, the tolerance of the polygonatum cyrtonema protoplast under high-temperature stress is improved.

The invention relates to a palm crop protoplast gene editing method based on a CRISPR / Cas9 system and application of the palm crop protoplast gene editing method, and belongs to the technical field of biology. A carrier containing PDS gene editing targets and Cas9 protein genes of palm crops is successfully delivered to protoplasts of the palm crops such as coconuts and areca nuts through protoplast transformation for the first time, and the carrier contains sgRNA sequences designed according to PAM sequences recognized by Cas9 protein. The sgRNA and Cas9 compound formed after expression in palm crop protoplast cells can be edited at a PDS gene editing target. According to the method disclosed by the invention, an editing carrier based on the CRISPR / Cas system can be delivered into the palm crop protoplast, the method is used for gene editing technology research of palm crops such as coconuts, betel nuts, oil palms, date palms and the like, and the feasibility of the method for editing the palm crop protoplast genome target gene based on the CRISPR / Cas 9 system is verified; and a rapid and effective method is provided for subsequently establishing an efficient palm crop protoplast gene editing technical system.

The invention belongs to the technical field of leguminous plants, and particularly discloses a soybean stem protoplast preparation and transformation method, which specifically comprises the steps of soybean aseptic seedling culture, soybean stem protoplast preparation and protoplast transformation. According to the method, the protoplast is prepared from the stem part of the first internode of the soybean, the formula of an enzyme solution is optimized, the protoplast cleaned and collected by W5buffer is utilized for conversion, and PEG instantaneous conversion reaction conditions are utilized, so that the yield and the conversion rate of the soybean protoplast are improved, and the method is more beneficial to identifying the soybean gene function.

The invention discloses an application of a DsHAD gene in regulation and control of the growth, the reproduction, the pathogenicity and the stress resistance of Ddymeria segeticola. The DsHAD gene is derived from Ddymeria segeticola, and a knockout mutant is obtained by constructing a knockout gene segment of the DsHAD gene of the D. segeticola and introducing the knockout gene segment into a D. segeticola protoplast. It is found through experiments that the DsHAD gene plays a role in the growth, reproduction and adversity resistance of D.segeticola, and the pathogenicity of D.segeticola to tea tree leaves is remarkably reduced. Therefore, the DsHAD can be used as a bactericide target and a key protein of a disease pathogenic mechanism, is used for researching and developing medicaments for diseases caused by D.segeticola infection and cultivating new varieties of disease-resistant plants, and has a wide application prospect in prevention and treatment of plant pathogenic fungal diseases.

The invention discloses a preparation method of fresh taxus chinensis stem and leaf protoplasts. The preparation method comprises a preparation process and a purification process. The preparation method of the taxus chinensis stem and leaf protoplast is simple and easy to operate, raw materials are easy to obtain and low in price, reagent components have good biocompatibility, and the taxus chinensis stem and leaf protoplast is free of harmful components, safe and environmentally friendly. The invention provides a simple and rapid taxus chinensis stem and leaf protoplast enzymolysis preparation method, which adopts vacuum filtration, accelerates the permeation of enzymatic hydrolysate and improves the enzymolysis efficiency, so that the protoplast can be rapidly obtained. Meanwhile, high-purity separation of the protoplast is carried out in combination with density gradient sedimentation of the iodixanol solution, so that the protoplast with uniform size and complete form is obtained, and convenience is provided for scientific researches such as subsequent conversion and single cell transcriptome sequencing.

The present invention relates to the field of plant-derived cellular vesicles, and in particular of nanovesicles obtained from plant protoplasts. A method for their preparation and uses thereof in the medical, cosmetic and nutraceutical fields are described.

The invention discloses a separation and instantaneous conversion method of green Chinese onion leaf protoplast, and belongs to the technical field of protoplast preparation, the method comprises the following steps: 1) culturing green Chinese onion leaves; 2) separation and purification of green Chinese onion leaf protoplast; 3) determining the yield and vitality of the green Chinese onion leaf protoplast; 4) determining an optimal enzymolysis condition; and (5) PEG-mediated protoplast transformation: determining the optimal concentrations of cellulase, macerozyme and mannitol in the enzymolysis process, and determining the optimal enzymolysis time, so that the separated green Chinese onion leaf protoplast is higher in yield and activity, and the separated green Chinese onion leaf protoplast can not only be used for instantaneous transformation, but also can be used for enzymolysis of the green Chinese onion leaf protoplast. And a technical support is provided for research on molecular biology of the green Chinese onions.

The invention discloses a method for improving biocontrol photosynthetic bacteria through a far-source hybridization and genome rearrangement technology. The method comprises the following steps: inoculating a rhodopseudomonas palustris GJ-22 seed into a photosynthetic bacteria liquid culture medium for culturing; inoculating the bacillus subtilis KY-2 into an LB liquid culture medium for culturing; the cultured GJ-22 is taken and subjected to treatment and centrifugation, and then a GJ-22 protoplast is obtained; kY-2 is taken to be treated, and KY-2 protoplast is obtained after centrifugation; fusing the obtained GJ-22 protoplast and KY-2 protoplast, diluting a photosynthetic bacterium hypertonic liquid culture medium, and regenerating on a photosynthetic bacterium soft agar culture medium to obtain a fused strain; a fusion strain is screened out through an induced tobacco core leaf anti-TMV test to serve as a starting strain for genome rearrangement. Preparing protoplast from the original strain; dividing the prepared protoplast into two groups, and respectively carrying out ultraviolet inactivation and thermal inactivation; respectively fusing the inactivated protoplasts, and regenerating to obtain fusant strains; finally, a target strain with genetic stability is screened through a fusant strain induced tobacco core leaf anti-TMV test.

The invention discloses a method for creating cadmium-resistant rice with low grain cadmium accumulation and application. The rice oshma3oslct1 double mutant expressing the Cd binding autophagy adapter protein AIM-SpMTL-GFP is constructed through CRISPR-Cas9, so that the transfer of Cd from xylem to phloem and the accumulation of Cd in root vacuoles are reduced, meanwhile, the Cd binding autophagy adapter protein AIM-SpMTL-GFP can also accumulate cadmium in protoplast vacuoles of leaves, the yield and quality of rice grains are not influenced, and the yield and quality of the rice grains are greatly improved. Cd accumulation of grains is reduced, and Cd is enriched into straws at non-edible parts, so that industrial treatment is facilitated. The cadmium-resistant rice with low grain cadmium accumulation can reduce cadmium pollution in a food chain, so that the harm of cadmium to a human body is reduced.

The invention relates to the technical field of plant cell engineering, and particularly discloses a plant protoplast semi-fusion breeding method which comprises the following three stages of seedling material selection, preparation, fusion, induction and cultivation. A large number of fused plant seedlings are obtained through a series of operations and used for large-scale planting. The method comprises the following steps: selecting excellent varieties of a plant A and a plant B, respectively preparing the plant A and the plant B into protoplasts in a preparation process, culturing the protoplasts into corresponding calluses, and performing half-cell fusion on a fusion part to obtain mosaic calluses. Then, the callus of the plant A to be used as the root is induced to locally induce adventitious roots, the callus of the plant B to locally induce adventitious buds, and finally, breeding is carried out to obtain the super seedlings. According to the method, the grafting principle is combined, the degree and conditions of cell fusion are optimized, the breeding cost is reduced, meanwhile, it is ensured that the fusion body can stably express the excellent characters of parents at different parts, and agricultural large-scale production is facilitated.

The invention discloses a method for enhancing squalene synthesis through oil tea HMGR gene editing. According to the method, phosphorylation regulation and control sites Ser47 and / or Thr208 recognized by MAPK are targeted, and enzymatic activity inhibition is relieved and metabolic flux is enhanced by constructing phosphorylation resistance mutants (such as Ser-> Ala and Thr-> Val). The constructed editing vector can be introduced into camellia oleifera tissues in manners of PEG-mediated protoplast transient transfer, agrobacterium infection of calluses and the like, so that stable expression is realized. Experiments prove that the enzyme activity of HMGR in the editor is remarkably improved, the squalene accumulation amount is increased by 50% or above, the editing efficiency is high, expression is stable, and the method is suitable for high-value development of plant triterpenoid functional components and excellent strain breeding. The method also has the potential of popularization and application in other triterpenoid synthetic crops.

The invention discloses a method for site-specific replacement or insertion of DNA fragments in a plant genome, and belongs to the technical field of biology. The method disclosed by the invention comprises the following steps: editing a genome of a plant protoplast by adopting a CRISPR / Cas9 system and taking a double-stranded DNA repair template as a donor template to obtain an edited protoplast; and carrying out culture regeneration on the edited protoplast by utilizing a plant protoplast transformation method to obtain a site-directed mutagenesis plant in the genome so as to realize site-directed mutagenesis of the target plant genome. The method disclosed by the invention can be used for accurately replacing DNA in a plant genome at a fixed point. According to the invention, a recognition sequence of recombinase can be accurately inserted into a genome at a fixed point through homologous recombination, and then accurate fixed-point replacement of a gene large fragment is realized by virtue of a specific recombinase system.

The invention relates to the field of microbial genetic transformation, and discloses a preparation method of Ilyonectria robus fungus protoplast and a construction method of a genetic transformation system.The preparation method comprises the following steps that S11, I.robus hyphae are prepared, after I.robus is cultured on a PDA solid culture medium for 14 days, the I.robus hyphae are inoculated to a CMC liquid culture medium to be subjected to shake-flask culture, and spores are filtered and collected; diluting the spore concentration, inoculating into a YEPD liquid culture medium, carrying out shake-flask culture, filtering and collecting hyphae; and S12, preparing enzymatic hydrolysate, namely adding the hyphae prepared in the step S11 into the enzymatic hydrolysate prepared in the step S12, carrying out shake flask enzymolysis, filtering, centrifuging filtrate, discarding supernate, adding a 0.7 M NaCl solution, centrifuging, and discarding the supernate, so as to obtain the I.robus fungus protoplast. The protoplasts with proper quantity and good quality can be efficiently obtained, and the transformation efficiency of a subsequent genetic transformation system is improved; and the constructed genetic transformation system can be used for construction of a mutant library of the I.robusta strain, interaction of a host plant and the I.robusta strain and research on the pathogenesis of the I.robusta strain.

The invention discloses a mining method and application of a new functional gene of asexual reproduction filamentous fungi, and relates to the technical field of applied microorganisms, and the preservation number is CGMCC No.12166. The preservation unit is China General Microbiological Culture Collection Center; the preservation date is April 11, 2016. The invention discloses a mining method of a new functional gene of asexual reproduction filamentous fungi. The mining method comprises the following steps: S1, constructing a uridine synthesis defect type trichoderma strain A with hygromycin resistance; s2, obtaining a random mutant strain B; s3, constructing a temporary simulated diploid through a protoplast fusion method; s4, carrying out random complementation on chromosome genes of the random mutants through homologous recombination of the two sets of chromosomes in the nucleus, and screening through phenotypic change to obtain phenotypic complemented mutants; and S5, determining a key mutation site for back-complementing by using a re-sequencing technology, and reversely verifying the gene function by using a gene editing method. The method disclosed by the invention provides a new method for researching functional genes of asexual reproduction type filamentous fungi.

The invention provides an efficient and stable nori pythium gene transformation method which comprises antibiotic concentration screening, protoplast preparation, transformation system optimization, positive transformant screening and transformant phenotype and gene expression stability analysis, and the evaluation method is the nori pythium gene transformation method disclosed for the first time in China. The method provides a valuable tool for researching gene functions and pathogenic mechanisms of pythium aphanidermatum in the future.

The invention discloses a genetic transformation method of PEG (Polyethylene Glycol) mediated penicillium sclerotiorum strain protoplast and application of the genetic transformation method. The method comprises the following steps: (1) culturing penicillium sclerotiorum to produce spores, germinating hyphae, and collecting and cleaning fresh penicillium sclerotiorum hyphae; (2) compounding Lysing Enzymes, Yatase, cellulase and pectinase to prepare a protoplast enzymatic hydrolysate, and then adding the protoplast enzymatic hydrolysate into the penicillium sclerotiorum hypha for enzymolysis to obtain a penicillium sclerotiorum protoplast; and (3) adding plasmids to be transformed into the penicillium sclerotiorum protoplast, and carrying out PEG (Polyethylene Glycol) mediated transformation and resistance screening to obtain a penicillium sclerotiorum protoplast transformant. According to the method, the number and regeneration rate of the protoplast can be remarkably increased, and the enzymolysis time can be effectively shortened.

The invention provides an efficient double-base editing system without PAM (Pulse Amplitude Modulation) limitation. According to the technology, different point mutations are introduced into SpRY, the SpRY is fused with efficient adenine deaminase and cytosine deaminase to construct a double-base editor, and efficient PAM-limitation-free double-base editing is achieved in the rice protoplast. According to the plant basic group editing method, efficient and PAM-less double basic group editing can be achieved, and the plant basic group editing technology is wider in target range, higher in efficiency and higher in double basic group editing product proportion.

The invention discloses a 7-aminocephalosporanic acid producing strain as well as a preparation method and application thereof. The invention provides a method for preparing 7-aminocephalosporanic acid. The method comprises the following steps: carrying out combined plasmid construction on promoters from different sources and a plurality of cephalosporin C acyltransferases; a cephalosporin C acyltransferase gene is integrated into a cephalosporium acremonium strain genome through a protoplast transformation method to obtain recombinant bacteria, and the recombinant bacteria are fermented to obtain a 7-aminocephalosporanic acid product. According to the invention, CPC high-productivity cephalosporium acremonium strain is taken as a starting strain, and cephalosporin C acyltransferase is introduced into the starting strain in an exogenous manner by means of genetic engineering, synthetic biology and the like, so that direct production of 7-aminocephalosporanic acid from the engineering strain can be realized. The method has the advantages of simple steps, no chemical pollution in the production process, and huge industrialization prospect.

The invention discloses a streptomyces gilvosporeus strain with high natamycin yield and application thereof, and belongs to the technical field of industrial microorganisms. The phosphate-tolerant natamycin high-yield strain GR-2 is successfully bred by combining protoplast fusion with phosphate tolerance screening, and the yield of natamycin produced by fermentation of the strain in a shake flask can reach 1.81 g / L and is 2.26 times that of an original strain; the fermentation yield in a fermentation tank reaches 13.9 g / L, the passage stability is good, the industrial production requirement can be met, and wide application prospects are achieved.

As a main grain crop in the world, the yield and quality of rice are often threatened by bacterial leaf blight and rice blast. According to the invention, a recombinant expression vector for expressing STTM-miR169d / p is constructed by starting from regulation and control of the expression quantity of miRNA through an STTM technology. By adopting an agrobacterium tumefaciens-mediated rice protoplast transformation technology, a rice plant containing the STTM-miR169d / p transgenic fragment is obtained. Through field inoculation of xanthomonas oryzae pv. Oryzae and inoculation of rice blast spores on in-vitro leaves, it is verified that the STTM-miR169d / p transgenic rice has significantly improved resistance to xanthomonas oryzae pv. Oryzae and rice blast compared with wild type rice. The invention not only provides a new germplasm resource for cultivating disease-resistant rice, but also provides a new thought for regulating and controlling the disease resistance of rice from the aspect of miRNA.

The invention discloses an application of a protoplast CUAmp; the invention discloses a high-throughput method for detecting a transcription factor recognition motif by using a Tag technology. The method comprises the following steps: 1) cutting up plant leaves, soaking the cut plant leaves in enzymatic hydrolysate, and separating cells, so that rice powder is completely soaked in the enzymatic hydrolysate; 2) after enzymolysis, wrapping filter residues with gauze, and extruding the filter residues to collect protoplast cells; (3) adding the protoplast cells into a centrifugal tube containing plasmids, instantaneously transferring the plasmids into the cells, and expressing genes transferred by the plasmids; (4) adding a GFP antibody, a secondary antibody and a Tn5 transposon, recovering the DNA combined with the GFP antibody, and carrying out subsequent NGS library building; 5) performing high-throughput sequencing to obtain a transcription factor sequence, and performing biological information analysis to obtain a transcription factor combined motif; and 6) verifying the interaction of the transcription factor and the recognition motif by using an EMSA method.

The invention relates to the field of plant genetic engineering, in particular to a method suitable for preparation and transformation of leaf protoplasts of all plants under brassica. By changing the selection and dosage of the enzyme and the formula of the enzymolysis buffer solution, the protoplast preparation requirements of different plant leaves under brassica are met. By changing the formula of the MMG solution and the conversion solution and the conversion method, the protoplast conversion requirements of leaves of different plants belonging to brassica are met. The method can be suitable for leaves of different degrees, special treatment is not needed, and the protoplast conversion efficiency of leaves of different plants under brassica is improved through a simple and convenient centrifugal mode.

The invention discloses an application of DspckA in regulation and control of the pathogenicity of Didymeria segeticola and application of the DspckA as a bactericide target. The DspckA gene disclosed by the invention is derived from D.segeticola, and a knockout mutant is obtained by constructing a knockout gene segment of the DspckA gene and introducing the knockout gene segment into a D.segeticola protoplast. It is found through experiments that the DspckA gene plays a role in the pathogenicity of D.segeticola and the sensitivity of the DspckA gene to ningnanmycin. Molecular docking analysis and trace thermophoresis experiments prove that the DspckA protein can be used as a bactericide target and has interaction with ningnanmycin, and amino acids at T264, T265 and E306 are key binding sites. The DspckA can be used as a bactericide target and a key protein of a disease pathogenic mechanism, and has a wide application prospect in prevention and treatment of plant pathogenic fungal diseases.

The present invention relates generally to the field of molecular biology and concerns a method for high throughput screening and characterization of one or more candidate plant pathogen effect genes and / or one or more candidate plant disease resistance genes in a single plant cell, the method comprises: (i) introducing into a plant protoplast one or more expression cassettes comprising at least one candidate plant pathogen effect gene and / or at least one candidate plant disease resistance gene; (ii) transiently expressing the one or more candidate plant pathogen effect genes and / or the one or more candidate plant disease resistance genes; (iii) measuring reactive oxygen species (ROS) produced by the protoplast; then (iv) separating the plant protoplast producing the ROS; and (v) identifying the introduced at least one candidate plant pathogen effect gene and / or at least one candidate plant disease resistance gene. Alternatively, the protoplast is capable of expressing the selectable marker, thereby allowing for the measurement of modulated selectable marker expression as an alternative indicator for HR activation.

The invention is applicable to the field of protoplast extraction application, and provides an enzymatic hydrolysate for Leymus chinensis protoplast extraction, which comprises the following components: 2.5-5% of cellulase R-10, 0.5-1% of macerozyme R-10, 0.7-1 M of mannitol, 5-9 mM of potassium chloride, 10-20 mM of MES buffer solution, 10-20 mM of calcium chloride, 0.05-0.2% of BSA, and the balance of ultrapure water. The invention also provides a preparation method and an application method of the enzymatic hydrolysate. In conclusion, according to the particularity of the leymus chinensis cell wall, an enzymatic hydrolysate formula is optimized, extraction conditions are adjusted, cell damage is effectively reduced, higher protoplast activity and quantity are obtained in leymus chinensis extraction, the extraction efficiency of the leymus chinensis protoplast is remarkably improved, and further conversion and gene expression research requirements are met.

The invention discloses a plant gene editing system and method for delivering CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 based on mobile RNA (ribonucleic acid). The system does not depend on tissue regeneration or protoplast culture, Cas9 and gRNA are remotely delivered from the root to enter the overground part by fusing a CRISPR / Cas9 gene editing carrier of a tRNA-like sequence (TLS) movable motif and combining a hairy root transformation system, target gene editing is completed, and heritable gene editing seeds can be obtained at the current generation. The method comprises the following steps: 1, constructing a movable CRISPR / Cas9 gene editing fusion vector, wherein the movable CRISPR / Cas9 gene editing fusion vector comprises a target vector, an intermediate vector and an editing vector; and 2, application of the agrobacterium rhizogenes mediated plant hairy root transformation system. And 3, detecting the mobility and editing type of the movable editing carrier. And 4, gene function verification application. The system greatly breaks through the bottlenecks of difficult plant genetic transformation and long time consumption of precise breeding, and provides theoretical basis and technical support for gene function research and germplasm innovation of important agronomic traits of plants.

The invention provides a wheat powdery mildew resistance gene TaGAP1 as well as an encoding protein and application thereof. The TaGAP1 is silenced through a BSMV-mediated instantaneous silencing technology, after powdery mildew E09 is inoculated, the number of powdery mildew colonies of a silenced plant is obviously increased, the powdery mildew grows and develops faster than that of a control plant, and it is indicated that the silent TaGAP1 reduces the resistance of wheat to powdery mildew; the bacterial colony area and biomass of powdery mildew of overexpressed TaGAP1 are remarkably reduced, and the resistance of wheat to powdery mildew is remarkably enhanced, so that TaGAP1 can be used as an effective gene resource for improving the powdery mildew resistance of wheat. Tobacco transient expression and wheat protoplast localization show that the TaGAP1 protein is localized in cytoplasm and cell nucleus. The wheat powdery mildew-resistant gene TaGAP1 provided by the invention provides a new gene resource for cultivating powdery mildew-resistant wheat varieties, and has important application value for widening the genetic basis of wheat and genetically improving the powdery mildew resistance of wheat.