5 results about "Pseudolarix" patented technology

The invention provides a traditional Chinese medicine composition for preventing hair loss, growing hair and removing dandruff as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The invention discloses a traditional Chinese medicine composition for preventing hair loss, growing hair and removing dandruff. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 8-12 parts of lithospermum, 8-12 parts of paris polyphylla, 15-25 parts of duckweed, 8-12 parts of golden larch bark, 8-12 parts of lucid ganoderma, 15-25 parts of cocklebur fruit, 15-20 parts of gallnut, 8-12 parts of radix sophorae flavescentis, 5-10 parts of schizonepeta, 8-12 parts of radix saposhnikoviae, 4-8 parts of notopterygium root, 5-10 parts of schisandra chinensis, 5-10 parts of elecampane, 5-10 parts of eclipta, 5-10 parts of ginseng, 5-10 parts of angelica sinensis, 5-10 parts of saponin, 5-10 parts of soapberry and 5-10 parts of broadleaf holly leaf. , 8-12 parts of fructus ligustri lucidi, and 4-8 parts of herb of wiss mannii The invention provides a traditional Chinese medicine composition for preventing alopecia, growing hair and removing dandruff, aiming at improving the problems of alopecia, dandruff and scalp discomfort.

This invention discloses a stable and efficient method for preparing protoplasts from *Pseudolarix amabilis*. The method directly uses etiolated *Pseudolarix amabilis* seedlings as material to prepare protoplasts, requiring no other aseptic procedures, and the entire process is simple and convenient. Furthermore, by setting different buffer component ratios and a specific mannitol concentration, this invention achieves stable maintenance of the osmotic pressure of *Pseudolarix amabilis* seedling cells during the separation process. Experimental verification shows that the protoplast yield using this method is 1.32 × 10⁻⁶. 5 The protoplasts were prepared at a density of 1 protoplast per ml, with 95% of them intact and undamaged, resulting in significantly improved quality and activity. Furthermore, the enzymatic hydrolysate described in this invention overcomes the difficulty of enzymatic hydrolysis caused by the high degree of leatheriness in *Pseudolarix amabilis* leaf tissue. This method is suitable for widespread application in the field of herbaceous plant biotechnology.

The invention provides a composition for treating chronic wounds and a preparation method and application thereof, and belongs to the technical field of chronic wound treatment. The composition for treating the chronic wound surface, provided by the invention, is prepared from the following raw materials in parts by weight: 8 to 12 parts of brown sugar, 8 to 12 parts of rhizoma coptidis, 8 to 12 parts of radix ampelopsis, 12 to 18 parts of cortex ilicis rotundae, 10 to 12 parts of radix sanguisorbae and 0.5 to 1.5 parts of resina draconis. The composition for treating chronic wounds provided by the invention is rigorous in compatibility and distinct in monarch, minister, assistant and guide. Brown sugar and coptis chinensis are used as monarch drugs; the ministerial drugs ampelopsis japonica and ovateleaf holly bark help the monarch drug to clear damp and promote tissue regeneration; sanguisorba officinalis and dragon's blood are used as adjuvant drugs for removing blood stasis and activating blood to promote wound healing. All the components are combined to jointly achieve the effects of clearing heat, eliminating dampness, promoting blood circulation and promoting tissue regeneration, and are exactly combined with the pathogenesis of damp-heat stasis resistance and difficulty in healing wounds, so that the traditional Chinese medicine composition is suitable for treating chronic refractory wounds, pressure sores, non-healing wounds after operations, chronic eczema, bedsores and other durable and unhealed symptoms, and also has the effect of inhibiting pathogenic bacteria.

This invention discloses a traditional Chinese medicine ointment containing antibacterial, blood-activating, and tissue-regenerating components and its preparation method. It is prepared from the following raw materials in the indicated weight ratios: Coptis chinensis 8-12 parts, Phellodendron chinense 4-8 parts, Scutellaria baicalensis 4-8 parts, Polygonum cuspidatum 8-12 parts, Lonicera japonica 10-20 parts, Pseudolarix amabilis bark 8-12 parts, Lithospermum erythrorhizon 8-12 parts, Rheum palmatum (processed with wine) 7-11 parts, Sanguisorba officinalis 8-12 parts, Borneol 2-4 parts, Scolopendra subspinipes powder 1-3 parts, Salvia miltiorrhiza 10-2 parts. 0 parts, Angelica sinensis 10-20 parts, Spatholobus suberectus 10-30 parts, Dragon's blood 6-10 parts, Artemisia capillaris 8-12 parts, Frankincense 4-8 parts, Myrrh 4-8 parts, Rhubarb (processed with wine) 7-11 parts, Bletilla striata 8-12 parts, Catechu 8-12 parts, Calcined gypsum 10-20 parts, Lithospermum erythrorhizon 8-12 parts, Sanguisorba officinalis 8-12 parts, Centella asiatica 10-20 parts, Astragalus membranaceus 30-50 parts, Angelica dahurica 8-12 parts, Borneol 2-4 parts. This herbal ointment of the present invention achieves the effects of promoting the healing of chronic, refractory wounds, shortening healing time, and reducing scar formation.

The application discloses a method for establishing a high-efficiency regeneration system of Pseudolarix kaempferi somatic embryos, which comprises the following steps of seed disinfection and germination, induction of embryogenic callus, induction and proliferation of embryogenic callus sprouting, rooting culture and the like. The method can not only solve the problem of low proliferation efficiency in the current tissue culture technology, but also realize mass breeding of high-quality seedlings, and is more beneficial to laying a foundation for the researches on the preservation, propagation, genetic improvement and increase of the yield of secondary metabolites of the Pseudolarix kaempferi excellent germplasm resources.