276 results about "Meristem" patented technology

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.

The present invention provides compositions and methods for regulating expression of isolated nucleotide sequences in a plant. The compositions are novel nucleic acid sequences for regulatory elements providing expression preferentially in the inflorescence meristem and developing floral tissues. Methods for expressing an isolated nucleotide sequence in a plant using the regulatory sequences are also provided, as well as expression constructs, vectors, and transformed cells and plants.

Methods for transforming plants, particularly commercially important elite maize inbreds, are provided. The methods involve transformation of meristematic organogenic tissue or immature embryos, and include the use of defined plant growth media. The methods disclosed provide more stable transgenic plants, and permit the transformation of varieties of cereals that are not amenable to transformation by conventional approaches.

The invention relates to the technology of delaying growth of plants and discloses an old and famous tree growth retardant, a conveying method thereof and a conveying device thereof. The old and famous tree growth retardant mainly comprises paclobutrazol, chlormequat chloride, daminozide and water and inhibits the biosynthesis of GA in plant bodies to inhibit the growth of plant sub-apical meristems, delay the elongation and growing speed of cells and then delay the growth. The growth retardant is injected into the tree bodies through a conveying device with simple and safe operation, and theenvironments and safety of special occasions, such as classical gardens and the like, are ensured. A cylindrical sponge body of an absorber is embedded in a tree trunk, so the maximal absorption areais achieved after the retardant is infused into the tree trunk, the retardant can be uniformly and quickly diffused into conveying and guiding tissues on the peripheral surfaces of round holes, the absorption of the infused liquid is quickened. The liquid in the holes can be effectively prevented from losing due to a rubber hole plug of the absorber, so the utilization efficiency of the retardantis improved.

The present invention relates to an efficient method of in-vitro micropropagation of Piper longum plants. In particular, the present invention is directed towards the novel method for micropropagation of Piper longum from lateral bud (meristematic) explant (starting material), by culturing the explants on different medias. This method results in mass production of the plant Piper longum in a short span of time.

The invention relates to plant cultivation field, particularly a method for culturing the detoxified seedling by the sweet potato stem tip. The method comprises following steps: firstly selecting qualified potato seed for raising seedling, scissoring the photo seedling stem tip, peeling the stem tip meristem for culturing, seedling formation cultivation after the stem tip meristem enlarges and turns green, performing the dwarf shoot cottage cultivation to get the test-tube plantlet, virus detection, taking the detoxified seedling leaves for inducement rapid propagation, domestication and transplantation, obtaining the stem tip seedling of the breeder's seed potato. The invention has simple art, utilizes the detoxified seedling leaves inducement rapid propagation technology to obtain multiple detoxified seedlings of one strain in a short period so as to lower production cost, effectively improve the virus detoxified rate and stem tip establishing percent and rapidly provide the nontoxic seedling for the sweet potato production and resource storage.

The invention provides a fresh bamboo nutrient solution, which belongs to the technical field of bamboo wine preparation. The invention overcomes the problems that most bamboo plants cannot survive for more than three years and an ideal productive life of a bamboo wine cannot be reached in conventional bamboo wine preparation methods. The fresh bamboo nutrient solution provided by the invention mainly comprises, by weight, 25 to 32% of a plant-growth regulator, 23 to 28% of beneficial organisms, 22 to 28% of a PK nutrient solution and 18 to 25% of a trace element fertilizer. The fresh bamboo nutrient solution can effectively improve activity of a variety of enzymes in fresh bamboo plants in the process of metabolism and activity of meristem in the period of fresh bamboo, increases chlorophyll content, enhances a net photosynthetic rate, promotes growth of roots on bamboo rhizome, enables fresh bamboo to resist external stimulation like methanol and ethanol in the wine and to have survival time of more than three years and allows the bamboo wine to be better prepared.

The invention discloses a teapot dates tissue culturing method. The method comprises the steps of clipping teapot dates with a bud stem tip in a large land; cleaning the teapot dates with tap water; putting the teapot dates into alcohol to sterilize; sterilizing by using mercury bichloride; cleaning the teapot dates by using sterile water; inoculating into a starting culture medium; transferring to a meristem culture medium; transferring to a successive transfer culture medium; and finally, finishing the establishment of the whole tissue culture system in a root medium so that the teapot dates tissue is subjected to sterile culture and transplantation under a manually-controlled illumination and temperature condition. In-vitro rapid propagation of the teapot dates is carried out according to the embodiment of the invention, the process from incubation to rooting needs 70 days, the teapot dates tissue can be transplanted to a seedling culture bed on the ground, the induction survival rate is up to 97 percent, the increment reaches 10 times, and the transplantation rooting rate is also up to 95 percent.

The present invention relates to expression cassettes comprising transcription regulating sequences with meristem-preferential or meristem-specific expression profiles in plants obtainable from Arabidopsis thaliana genes At2g02180, At5g54510, At2g26970, At2g01180, At3g45560, At4g00580, At1g54480, or At4g11490, or the Arabidopsis thaliana genomic sequences as described by SEQ ID NO: 35 or 36.

The invention discloses a detoxification, tissue culture and rapid propagation method of common turmeric, which comprises the explant selection and treatment, induction culture, the virus identification of plant regeneration, proliferation culture, transplanting and other steps. In the invention, on the basis of the MS culture medium, the plant growth regulators such as 6-benzyladenine, kinetin, Alpha-banai acid, adenine sulphate are added for the induction culture of common turmeric apical meristem and the proliferation and rooting of multiple shoot two key stages. With the adoption of the packaged technology and method provided in the invention for the tissue culture and rapid propagation of common turmeric, the difficult problems of common turmeric such as virus accumulation, declining quality and yield reduction caused by propagation coefficient and technical stability are met. Therefore, the invention can provide a seedling-raising method with short cycle, high rate of reproduction, low-cost for the factory production of common turmeric.

The present invention relates to the technical field of Luo Han Guo cultivation, in particular to a method for cultivating Luo Han Guo. The branches are collected and sterilized, and the stem apex meristem is cut out for subculture; the seedlings are selected and quickly dipped in IBA, and the lower 1 / 4 of the seedlings are inserted into the Between two pieces of filter paper, clamp the filter paper with two aluminum alloy bedding bars, and fix the aluminum alloy beading bar with a small clip, then hang the aluminum alloy beading bar on the two walls of the rooting incubator for rooting cultivation, and the rooting incubator contains a box volume of 1 / 4 or 1 / 2 MS culture solution; before transplanting, apply quicklime to the planting site, and then apply decomposed farmyard manure, place the seedlings in the planting nest and pour enough water for rooting, after 7 days, wet the fertilizer once a week, When the Luo Han Guo seedling grows more than 3 true leaves, remove the film covered on the Mirs, and apply organic compound fertilizer, the method of the present invention obtains transplanted seedlings, the stems are strong, the rooting rate is high, the quality is good, and the survival rate of field transplantation is high. High and adaptable.

A method for producing the bulbs of Garlic with saving the cost for producing them and enhancing the work efficiency and the yield by dark-culturing and / or liquid media-culturing of the garlic tissues in vitro is provided, which comprises the steps of:a) isolation and excision of the virus-free tissues in the length of 0.2 to 0.3 mm obtained from the meristem of parent body of garlic;b) inoculating the excised tissues from the meristem tissue of garlic onto the solid-type primary media;c) culturing the tissues inoculated onto the solid-type primary media under the light condition at 25° C. in the culturing room for 4 weeks;d) propagating the shoots regenerated from the cultured tissues at the multiplication media for 4 weeks;e) transferring the propagated shoots into the liquid-type media with additional components of 90 g / l of sucrose and plant growth regulators and culturing them primarily for 10 days;f) transferring the primarily cultured tissues into the liquid-type media having the same composition as the media used in the step e) with additional components of 140 g / l of sucrose and plant growth regulators;g) secondarily culturing the said tissues at about 25° C. and under the dark-condition in the culturing room for 6 weeks;h) harvesting the microbulbs from the virus-free garlic plants in vitro;in which the steps f) and g) are carried out in the altered liquid-type MS media under the dark-condition with no artificial illumimation.

The present invention relates to in planta transformation methods for plants or plant materials with a genetic construct, wherein at least one meristematic cell of a meristematic tissue of an immature inflorescence able to differentiate into a gamete of a pollen or of an ovule is exposed and then transformed, wherein the transformation can be performed to yield a stable integration or to yield a transient introduction of a genetic construct of interest. Further provided are methods for manufacturing a transgenic plant, or methods for manufacturing a genetically manipulated plant based on the in planta transformation methods according to the present invention. In addition, there is provided a plant or a progeny thereof manufactured according to the transformation and / or manufacturing methods according to the present invention.

The invention belongs to the technical field of cell engineering, and specifically relates to a method for obtaining Lolium L. embryogenic callus and maintaining regeneration capacity of the callus. According to the present invention, the material for callus induction is the mature seed embryos of the Lolium L.; the mature embryos are adopted to induce the callus, after the mature embryos are cultured for 1 month, 4 secondary cultures are performed, wherein the time of the 4 secondary cultures is 2 months, and the secondary culture is performed every 2 weeks; a differentiation regeneration culture treatment is performed on the callus, the differentiated plant is continuously subjected to the secondary culture, and the callus incapable of differentiation is discarded; the stipe meristem area of the secondary culture plant after regenerating is subjected to the second callus induction, wherein the culture time is 1 month; the callus is subjected to the secondary culture for 2 months, wherein the culture media is changed every two weeks; then the differentiation regeneration culture treatment is performed on the callus, wherein the callus incapable of differentiation is discarded; a long-term secondary culture is performed on the regenerated plant, when the callus is required to carry out the relevant research, the stipe meristem area of the plant is subjected to induction and secondary culture to obtain the required callus, wherein the regenerated plant is subjected to the second differentiation. According to the present invention, the operation is simple and feasible, and the stable and high-frequency differentiation regeneration efficiency can be obtained.

The invention discloses a compound insecticide of bacillus thuringiensis, verticillium lecanii and aconite alkaloid and a preparation method thereof. The compound biotic insecticide is mainly mixed through the fermentation liquor of bacillus thuringiensis and the meristem of the verticillium lecanii or blastospore and the aconite alkaloid with proper auxiliary agent according to certain proportion, wherein the mass percentage of the main medicine and the auxiliary agent respectively accounts for 50 percent to 90 percent and 10 percent to 50 percent. The compound insecticide has the advantagesthat the compound insecticide is broad-spectrum, has control effect to various crop pests, and particularly has obvious effect to homoptera pets, lepidoptera pets and coleopteran pets; the desinsection speed is high; the lasting period is long; pets are difficult to develop drug resistance; the compound insecticide is safe to people and animals, environment and natural enemies; the manufacturing process is simple, the material sources are wide, and the cost is low; and the preparations are multiple, and the application field is wide.

The present invention relates to expression cassettes comprising transcription regulating sequences with meristem-preferential or meristem-specific expression profiles in plants obtainable from Arabidopsis thaliana genes At2g02180, At5g54510, At2g26970, At2g01180, At3g45560, At4g00580, At1g54480, or At4g11490, or the Arabidopsis thaliana genomic sequences as described by SEQ ID NO: 35 or 36.

The invention discloses an in vitro conservation method of a color calla idioplasm resource, pertaining to the vegetable idioplasm resource conservation and tissue cultivation technical field. The method comprises such steps as preparation of a culture medium, disinfection of an explant and inoculation and induction culture, multiplication culture, root culture, a corm intumescence culture, idioplasm conservation culture, restore culture, and the detection and utilization of genetic stability, etc. The method adopts the meristem tissue of a 0.5mm stem tip of the color calla as the explant, a large quantity of elements in the culture medium are reduced and mannitol and penicillin are added, and a protocorm seedling is conserved at 10 to 15 DEG C under the surroundings of light intensity of 1000Lx for 10h / d, and after 15 months, the contamination of Erwinia is eliminated, the survival rate reaches 100 percent; the growth and multiplication are still normal without genetic variation. The method of the invention is characterized by low cost and simple operation etc., and can be popularized and applied in the idioplasm conservation field of the color calla.

The invention relates to an artificial culture method of halophila oralis seedlings for marine ecological restoration, and belongs to the technical field of artificial culture of marine plant seedlings. According to the method, halophila oralis is cultured indoors to quickly obtain high-quality seedlings according to the reproduction characteristics that stem nodes on creeping stems of the halophila oralis can meristem and bifurcate and the creeping stems can horizontally extend and grow; the method specifically comprises the step of placing pruned creeping stem sections in seawater in which nutrient solution and a plant growth promotion hormone are added to culture for 25-30 days until the creeping stems extend horizontally to newly increase 2-3 stem nodes. The method has the characteristics of simple required equipment, convenience in material source, low production cost, easiness in mastering of the technology, horizontal extension of the creeping stems and high growth speed of buds on the stem nodes, the defect that the growth and the reproduction of the halophila oralis are affected by natural factors can be overcome, a large number of halophila oralis seedlings can be quickly obtained, the growth speed is high after the seedlings are transplanted to a sea area, and the protection and restoration effects on the halophila oralis are obvious.

The invention provides methods of inhibiting the growth of a shoot apical meristem of a cucurbit rootstock plant, comprising contacting a shoot apical meristem of the cucurbit rootstock plant with an effective amount of a composition comprising one or more fatty alcohols, thereby inhibiting the growth of the shoot apical meristem of the cucurbit rootstock plant. The present invention further provides methods for preparing and producing cucurbit rootstock plants for grafting and methods for grafting. Additionally provided are cucurbit rootstock plants and plant parts and grafted cucurbit plants produced by the methods of the invention.

The invention discloses an OSMADS8 gene for cultivating dwarf transgenic plants; the nucleotide sequence of the OSMADS8 gene is between 109 and 852 from the 5' terminal in the Genbank, with number of U78892.1; the OSMADS8 gene can be also used for cultivating the transgenic plants with early-flowering characteristics. Besides, by RNAi technology, when OsMADS7 and OsMADS8 genes are simultaneously suppressed, transgenic rice lines are subjected to evident phenotype changes which are mainly shown as follows: the plants are dwarf and late-flowering, and the inner three-round floral organs all have the characteristics of partial palea and lose the determinacy of flower meristem.

Methods and compositions for plastid transformation and regeneration or development of transplastomic plants are provided. Embryo explants may be excised from seeds, and their meristematic tissue may be transformed directly without initiation of any callus phase before and / or after transformation. The present methods may be performed with fewer culturing steps relative to conventional methods, thereby enabling more rapid and efficient production of targeted transplastomic events in plants.

The invention relates to a method for promoting chrysanthemum to bloom in advance by using 5-azaC. The method comprises the following steps of: cutting and raising a seedling; after rooting, planting in a cultivation pot, and adopting the conventional cultivation management method to ensure water and fertilizer; and when a chrysanthemum plant has the height of 20-40cm, treating the apical meristem of the chrysanthemum by using the 5-azaC at the concentration of 200-500mu mol / l for 10 to 15 days, and restoring normal management. The method is easy to implement, has an obvious effect (the chrysanthemum can bloom 10 to15 days earlier), cannot generate any adverse effect on the enjoyment effect of the chrysanthemum, and is suitable for scale production in field.

The present invention relates to methods and hybrids for the targeted modification of a nucleic acid-target region in a plant target structure. The invention specifically relates to methods and hybrids for directly obtaining a plant or plant material which comprises an editing of a nucleic acid introduced in a targeted manner into a meristematic cell. The hybrids can be introduced in a transient and / or stable manner. The invention also relates to novel plant-optimized introduction strategies. The invention further relates to a method for carrying out an in vitro screening assay in order to first check the suitable gRNA candidates in vitro with respect to their efficiency.

The invention relates to the isolation and characterization of a maize gene, RAMOSA3 (RA3), responsible for meristem development and inflorescence development including branching. The gene, gene product, and regulatory regions may be used to manipulate branching, meristem growth, inflorescence development and arrangement, and ultimately to improve yield of plants. The invention includes the gene and protein product as well as the use of the same for temporal and spatial expression in transgenic plants to alter plant morphology and affect yield in plants. The invention also includes the gene and protein product for SISTER OF RAMOSA3 (SRA).

The invention provides a method for rapid propagation of Allium ampeloprasun var.ampeloprasun. The invention adopts an adventitious bud proliferation method to rapidly propagate Allium ampeloprasun var.ampeloprasun, makes use of apical meristem to get aseptic seedling and then makes use of root of the aseptic seedling to obtain a plurality of proliferatively adventitious buds. The invention avoids vitrification of tissue culture seedling resulted from dedifferentiation and redifferentiation during tissue culture and greatly improves propagation coefficient. The invention comprises the operation steps of disinfection of explant, aseptic seedling induction, adventitious bud induction, subculture multiplication, adventitious bud rooting and tissue culture seedling transplantation. The invention takes MS basic medium as a basis and adds plant hormone (Alpha-NAA, IBA, 6-BA, KT and GA) of different proportions in different combinations to reproduce new plants rapidly on an appropriate adventitious bud induction, multiplicated medium and a rooting medium. The method for rapid propagation of Allium ampeloprasun var.ampeloprasun of the invention has convenient operation, low cost and high application value.

The invention relates to a conjoined grafting method for plant meristem. The conjoined grafting method for the plant meristem is characterized in that the conjoined grafting method through meristem between different phylum, klasse, order, family, genus and species of plantae is provided, and the conjoined grafting method comprises an apcial meristem, a lateral meristem and an intercalary meristem. The meristem possesses massive reversible gene regulatory networks and spatial and temporal expression opportunities due to the fact that the meristem has high cell division ability and is a key tissue of organogenesis and morphogenesis and a starting point of organ and tissue differentiation. Therefore, conducting moderate manual injury intervention and conjoined grafting on the meristem can greatly increase opportunity of successful grafting, and enlarges boundary capable of being conducted grafting between different phylum, klasse, order, family, genus and species of the plantae. Due to the fact that scion and rootstock whole plant are not separated, bud mutation and fructification are promoted to obtain grafting progeny seeds, exchanging and changing of part genetic materials of grafting progeny can be achieved, and consequently, low coat and safe material exchanging of extra distant species of the plantae can be achieved, variation is induced, and then a new germplasm of a scion plant is created.

This invention relates to a method for the introduction of genes encoding desirable traits into both monocotyledonous and dicotyledonous plants and to plants and parts thereof produced by growing plants using this method. The time required for the production of transgenic plants is significantly decreased, while the number of transgenic plants is significantly increased. These increases are not dependent upon the use of super-virulent Agrobacterium strains. The invention also relates to an improved technique for in vitro regeneration of mono- and di-cotyledonous plants in a suitable medium containing a novel growth regulator regime that promotes cell elongation in the production of numerous somatic embryos that are regenerable into fertile plants.

The invention discloses a horseradish seedling culturing method. The horseradish seedling culturing method comprises the following steps: (A) rooting cultivation, (B) hardening seedling transplanting and (C) filed planting, wherein the rooting cultivation comprises the step that primarily cultured stem tip meristem tissue which undergoes sterilization and detoxification is transferred into a 1 / 2 Murashige and Skoog (MS) culture medium to grow, the hardening seedling transplanting comprises the step of transferring the tissue into a tray after the step (A) lasts for 28-32 days, and the field planting comprises the step of transplanting finished horseradish seedlings to fields after the step (B) lasts for 75-85 days. The method of adding rooting agents into the culture medium for enrichment culture is adopted to replace the method of using cytokinin, so that the problems that rooting is slow and roots are thin and long are solved, and the purpose of increasing the length of the root of a single seedling quickly in a short period is achieved. At the same time, the price of the rooting agents is far lower than that of the cytokinin. Compared with the auxin culturing method which is large in waste quantity and low in use ratio, the horseradish seedling culturing method is smaller in dosage, higher in use ratio and capable of greatly reducing cost.

The present invention relates to the preparation of plant cell cultures belonging to the Dendrobium Phalaenopsis, Anisellia, Polyrrhiza, Vanilla, Cattleya and Vanda genera and their use in cosmetic, nutritional and pharmaceutical fields. In particular, the present invention regards a selected cell culture of meristematic cells of plants belonging to the Dendrobium Phalaenopsis, Anisellia, Polyrrhiza, Vanilla, Cattleya and Vanda genera characterized in that it contains an amount of phenylpropanoids greater than 0.1% expressed on the cell dry weight and an amount of hydrosoluble polysaccharides greater than 5% expressed on the cell dry weight, as well as preparations, pharmaceutical and cosmetic compositions or food supplements containing said cell culture.