63 results about "Meristem" patented technology

The invention relates to the technical field of plant genetic engineering, and discloses a strawberry stolon regulation gene ZFP6 and application thereof, and the nucleotide sequence of the gene is as shown in SEQ ID NO.1. Researches prove that the ZFP6 gene is specifically expressed in axillary meristem of strawberry in the adult-plant stage, and the GA20ox4 gene can be directly or indirectly activated to start a biosynthesis pathway of GA and drive axillary buds to develop towards stolons. Through genetic interaction analysis of the ZFP6 gene, the GA20ox4 gene and the RGA1 gene, the function of the ZFP6 gene as an axillary bud fate decision upstream regulation factor is further verified, the blank of an endogenous regulation mechanism of the strawberry stolons is filled, and the ZFP6 gene is proved to be a molecular target for specifically regulating formation of the strawberry stolons. The gene sequence, the primer, the gene knockout expression vector and the matched technical system provided by the invention lay a foundation for strawberry molecular breeding and industrial application.

Compositions and methods for improving one or more agronomic traits of a plant are provided, which include the use of one or more active ingredients that are herbicides that kill, damage, or inhibit the growth of cells in the shoot apical meristem and optionally one or more surfactants, fungicides, bactericides, and / or pesticides. Use of the composition at specific vegetative growth stages of the plant, for example, between vegetative growth stage 1 (VI) and vegetative growth stage 6 (V6) is described.

A method for increasing yield or vigor of a plant is provided, which includes the selective removal of the plant's shoot apical meristem at a period between vegetative growth stage 1 and vegetative growth stage 2, or between vegetative growth stage 2 and vegetative growth stage 3.

A system, method, and apparatus for delivering genetic material to a shoot apical meristem. The delivery system may have a biolistic particle delivery assembly having a gas acceleration tube and a bombardment chamber. The bombardment chamber has a microcarrier launch assembly comprising an opening adapted to receive gas for acceleration of the microcarrier system and to direct gas to a target. The delivery system may have a source of pressurized carrier gas operatively connected to a first end of the gas acceleration tube; and an extender. The extender has a channel configured to fit through the opening of the microcarrier launch assembly. The length of the extender facilitates the penetration of the microcarrier system to an L2 layer of the shoot apical meristem, conferring germline transformation and / or modification.

This invention discloses a method for virus-free tissue culture using terminal buds of *Prunus armeniaca* rootstock, belonging to the field of plant tissue culture technology. The method includes: selecting terminal buds of *Prunus armeniaca* rootstock for pretreatment, sterilizing them with ethanol and mercuric chloride, and then immersing them in a virus-free pretreatment solution containing ribavirin and triazole nucleoside for virus removal; inoculating the virus-free terminal buds into WPM-based differentiation induction medium to obtain clustered buds; culturing the clustered buds in 1 / 2 MS-based proliferation subculture medium to obtain proliferating buds; inoculating the proliferating buds into 1 / 3 MS-based rooting medium to induce rooting; and finally, after hardening off and transplanting into a vermiculite-garden soil-humus substrate for acclimatization. This invention optimizes the virus removal and tissue culture system based on the characteristics of *Prunus armeniaca* rootstock, achieving a virus removal rate ≥95%, a terminal bud differentiation survival rate ≥85%, a rooting rate ≥90%, and a transplant survival rate ≥88%. This enables rapid and large-scale propagation of virus-free *Prunus armeniaca* rootstock seedlings, solving the problem of low propagation efficiency in traditional methods and providing high-quality rootstock support for the *Prunus armeniaca* industry.

Described herein are methods for producing a modified Rubus species plant or plant part. The method can include introducing a polynucleotide of interest (e.g., a portion of an editing system) and a gene that confers resistance to a selection agent into one or more meristem cells of a wounded tissue including meristem cells to provide a transformed tissue, culturing the transformed plant tissue in the presence of the selection agent in an amount at least 1.5 times that needed to kill 90% of Rubus spp. cells to provide a cultured transformed tissue, and regenerating a Rubus spp. plant or plant part from the cultured transformed tissue.

The invention discloses a soybean GmHRF-1 gene and application of protein coded by the soybean GmHRF-1 gene in improvement of soybean regeneration frequency and genetic transformation efficiency, and relates to the technical field of plant genetic engineering. The invention identifies and verifies that the GmHRF-1 gene in the soybean B3 transcription factor family has a brand new function of remarkably improving the regeneration capacity of somatic cells for the first time. By introducing the gene into soybean recipient cells and overexpressing the GmHRF-1, the regeneration frequency and genetic transformation efficiency of soybeans can be remarkably improved. The invention provides an efficient and universal endogenous gene for breaking through the technical bottleneck of genetic transformation of backbone germplasm which is difficult to regenerate of soybeans. The research result not only provides a new gene target for analyzing a molecular mechanism of soybean stem tip meristem regulation and regeneration, but also provides a technical support for constructing an efficient and stable soybean genetic transformation system.

The application provides application of a mutant maize ZmERECTA gene in regulation of intercalary meristem development and dwarf breeding. The full length of the maize ZmERECTA gene CDS is 2985 bp, and strong mutants er-1, er-2 and er-3 of ZmERECTA are obtained by EMS mutagenesis, and the plant height and ear height of the three are all reduced, which indicates that the gene is a gene that can be used for maize dwarf breeding. Taking er-3 as an example, the intercalary meristem development of the mutant is disordered, which indicates that the gene regulates the intercalary meristem development. Meanwhile, the 1306th base of the ZmERECTA CDS is mutated from (guanine) G to (adenine) A by EMS mutagenesis, and a weak mutant er-750 of EMS mutagenesis is obtained. The plant height of er-750 is obviously reduced relative to B73, and the ten-grain length and ten-grain width of er-750 are obviously increased and widened relative to er-1. It is indicated that the 1306th base of the ZmERECTA CDS is mutated from (guanine) G to (adenine) A, which can reduce the plant height, but the seed size is improved relative to the strong mutant, and it is a mutant site that can be applied to maize dwarf breeding.

The invention relates to application of a GbPIF3 gene functional structure domain in plant type regulation, which is characterized in that a plant type related gene Gb-PIF3 is obtained by cloning from an apical meristem in a hybrid F1 of sea island cotton-upland cotton, and the functional structure domain GbPIL of the gene is subjected to heterologous expression in arabidopsis thaliana through a transgenic technology. The photoresponse test and seedling property observation on the obtained transgenic material prove that the gene functional structure domain has the function in plant type regulation and control, can be used for transforming the plant type structure of upland cotton and regulating and controlling the flowering time, and has important significance on the improvement of the yield and quality of cotton, especially the genetic modification of close-planting cotton.

The invention discloses a Tongling white ginger stem tip detoxification and propagation method which comprises the following steps: (1) disinfecting the surface of a Tongling white ginger tuber, separating bud points, and inoculating the bud points into an induction culture medium for induction culture of adventitious buds; (2) stripping stem tip meristem from the adventitious buds induced in the step (1), inoculating the stem tip meristem into a regeneration culture medium, and synchronously inducing buds and roots to obtain a complete regeneration plant; wherein the size of the stem tip meristem is 1-2 mm; and (3) selecting the completely detoxified Tongling white ginger regenerated plant obtained in the step (2) as a propagation material, taking the stem node as an explant, and inoculating the Tongling white ginger regenerated plant into a proliferation culture medium for sterile propagation to obtain Tongling white ginger seedlings. According to the method, a special efficient detoxification regeneration technology system for Tongling white ginger is established, virus-free, high-quality and healthy seedlings are provided for industrial cultivation of Tongling white ginger, and accurate protection and inheritance of excellent germplasm resources of national geographical indication products are achieved.

The invention relates to the technical field of plant tissue culture, in particular to a sterile rapid propagation method of elaeagnus pungens stem tip meristem. The method comprises the following steps: immersing a stem section in a treating agent, and then washing with sterile water; inoculating the stem tip meristem into a primary induction culture medium which does not contain an exogenous antibacterial agent, and culturing to obtain sterile cluster buds; the method comprises the following steps: selecting robust rootless seedlings, and soaking the base parts of the seedlings in a solution containing IBA for pretreatment; then, the seedlings are inoculated into a rooting culture medium without exogenous auxin to be cultured, and complete regenerated plants are obtained; and hardening seedlings and transplanting. According to the invention, the long-acting silver ion / nano-silver composite protective layer is constructed, and the functional synergistic application of the protective layer in the whole process of subsequent primary culture, proliferation and seedling strengthening and rooting induction is realized; technical contradictions between efficient sterilization and explant activity maintenance, long-term sterile culture and endophyte pollution control, and high-quality root system induction and culture link disjunction in a traditional rapid propagation technology are synergistically solved.

The invention discloses an agrobacterium tumefaciens-mediated soybean sprout tip transformation method. The method comprises the following steps of explant infection and co-culture, bud induction and screening culture, elongation recovery and rooting culture and transgenic plant domestication culture. According to the method, the soybean sprout tips are selected as the explants, the soybean genetic transformation process is simplified, direct budding of apical meristem and synchronous rooting of underground parts can be achieved, only one month is needed from explant infection to obtaining of a complete regenerated transgenic plant with a developed root system, the transformation efficiency is high, the soybean transformation period is greatly shortened, and the method is suitable for large-scale popularization and application. The cost is effectively reduced. Meanwhile, the problem of soybean genotype limitation and the problem that northeast varieties bloom in advance in the tissue culture stage are solved, the method is applied to more than twenty main cultivated varieties difficult to transform, the transformation efficiency is high and stable, the highest efficiency can reach 81%, and reliable technical support is provided for soybean basic research and gene editing breeding work.

A method for increasing yield or vigor of a plant is provided, which includes the selective removal of the plant's shoot apical meristem at a period between vegetative growth stage 1 and vegetative growth stage 2, or between vegetative growth stage 2 and vegetative growth stage 3.

The invention relates to application of a cotton natural mutant DR as a receptor material in genetic transformation of cotton stem tips, and relates to the technical field of biology, the receptor material is a seed, tissue, cell or stem tip meristem of the cotton natural mutant DR, and the cotton natural mutant DR has dwarf and red leaf characters. According to the invention, the upland cotton natural mutant DR with dwarf and red leaf characters is used as a receptor material, and has outstanding characteristics in upland cotton stem tip genetic transformation; the method has the advantages that the method is particularly suitable for agrobacterium tumefaciens-mediated stem tip genetic transformation methods, and the transformation efficiency is stabilized between 8% and 13%; 2, the regeneration speed of the adventitious buds after genetic transformation is high, and positive adventitious buds can appear after about 15 days of induction; and 3, the method has stable morphological phenotypic characters of red leaves and short stems, integrates high transformation efficiency, rapid regeneration and unique plant type and leaf color characters, and provides an excellent acceptor material for cotton gene function research and germplasm innovation.

The invention relates to the technical field of plant biology, and discloses a lilium lancifolium virus-free seedling stem tip stripping culture method which sequentially comprises the following steps: (1) pretreatment: taking lilium lancifolium virus-carrying explants, carrying out first high-temperature stress treatment, then transferring into a sub-high-temperature condition, and carrying out second high-temperature stress treatment on the lilium lancifolium virus-carrying explants to obtain lilium lancifolium virus-free seedlings; carrying out shake culture by using a pretreatment culture medium containing plant stress resistance induction hormone; (2) stem tip stripping: after pretreatment in the step (1), stripping stem tip meristem with the size of 0.8-1.2 mm; (3) induction culture: the stem tip meristem stripped in the step (2) is inoculated to a solid induction culture medium for dark culture until callus is formed, and the solid induction culture medium contains cytokinin and an antiviral compound; through deep synergy of gradient temperature stress (high temperature to sub-high temperature) and specific stress-resistant hormone (BR) induction in time sequence and physiological functions, the physiological state of the explant in the pretreatment process is integrally regulated and controlled.

The invention belongs to the technical field of biological breeding, and particularly relates to a vegetable seed seedling cultivation method based on gene editing, which comprises the following steps: constructing a targeting nano delivery carrier loaded with gene editing ribonucleoprotein (RNP), the carrier is composed of surface modified polyethyleneimine and carbon nanodots of stem tip meristem homing peptide (MHP), and the carbon nanodots are uniformly distributed on the surface modified polyethyleneimine and the stem tip meristem homing peptide (MHP). RNP is delivered to stem tip meristem of a vegetable seedling through a micro-droplet in-situ permeation method, so that instantaneous editing of a target gene is realized, and homozygous editing seeds without exogenous gene residues are obtained through sexual reproduction. By means of the scheme, non-transgenic gene editing without genetic transformation and tissue culture is achieved, and the method has the advantages of being easy and convenient to operate, short in period, high in efficiency and wide in applicability and is suitable for character improvement of various vegetables such as tomatoes, spinach and cucumbers.

This invention belongs to the field of biotechnology, specifically relating to the cassava transcription factor MeWRKY30 and its applications. This invention cloned the transcription factor gene of MeWRKY30 from cassava. Overexpression of this gene in cassava can improve the resistance of transgenic cassava plants to low-temperature and drought stress. The effects of qRT-PCR on cassava... MeWRKY30 Gene expression was significantly upregulated after drought and treatment with the plant hormone abscisic acid (ABA). After recovery from low temperature treatment, the malondialdehyde content of the overexpressing cassava lines decreased, wilting was reduced, root growth inhibition was lessened, and the number of cortical cells in the root tip meristem was significantly increased.

Compositions and methods for improving one or more agronomic traits of a plant are provided, which include the use of one or more fatty acids that kill or inhibit the growth of cells in the shoot apical meristem and optionally one or more surfactants. Use of the composition between vegetative growth stage 1 (V1) and vegetative growth stage 6 (V6) is described.

The application discloses a cotton topping regulator with the functions of topping control and fruit promotion, and belongs to the technical field of crop plant growth regulators.The application adopts special compound active components and special inactive additive systems, does not add any exogenous hormones, insecticides, fungicides and other auxiliary materials, and constructs a differential target regulation mechanism.The regulator can act on the cotton apical meristem, flower bud differentiation tissue and phloem nutrient transport channel, and simultaneously realizes four functions of apical vigorous shoot inhibition, invalid bud removal, bud and boll shedding inhibition and photosynthetic product directional transport, thereby replacing artificial topping operation.The application can reduce the cotton bud and boll shedding rate by 18.2%-23.7%, increase the per mu seed cotton yield by 12.6%-19.3%, and simultaneously optimize the cotton fiber quality;the preparation has strong storage stability, is suitable for unmanned aerial vehicles, large-scale traction spraying machines and other full-process mechanized plant protection equipment, has a green and waste-free preparation process, meets the development needs of cotton simplified and green cultivation, and has high field popularization value.

The application relates to the field of plant genetic engineering, and specifically discloses a high-efficiency gene transformation system based on the stem tip meristem of a yellowing seedling of Zhongmian 113. The core components of the system include L1 medium suitable for Zhongmian 113, Agrobacterium GV3101 carrying a target gene, a special resuspension solution containing acetosyringone and Silwet-L77, and special primers for overexpression and gene editing; and the preparation method is as follows: seed disinfection and germination are performed to obtain a yellowing seedling, the stem tip is cut in an X shape to expose the meristem, Agrobacterium infection is performed, and then dark culture and light culture are performed, and after transplanting and cultivation to the true leaf stage, positive plants are screened and identified through PCR; the system can be used for gene transformation of Zhongmian 113 and improvement of high yield, high quality, disease resistance and the like, and has the advantages of strong adaptability, high transformation efficiency and short cycle; in addition, the preparation method of the application does not need callus regeneration, is simple and convenient to operate, has a standardized process, is good in repeatability and stability, and can quickly realize transgenic seed acquisition.

The application discloses a direct organogenesis genetic transformation method based on spinach stem tips and axillary buds, and relates to the technical field of plant genetic transformation. pCAMBIA1305-GUS As a plant expression vector, the Agrobacterium GV3101 (pSoup-p19) is used as a receptor Agrobacterium to establish a stable genetic transformation system of spinach. The application utilizes the regeneration capacity of the spinach stem tip meristem and axillary bud meristem, can improve the regeneration efficiency and transformation efficiency, and significantly shortens the genetic transformation cycle. The system can be used for the function research of key regulation genes of agronomic traits of spinach and the cultivation of excellent spinach varieties.

The invention relates to the technical field of plant cultivation, in particular to a top grafting and seed changing method and application thereof. The invention provides a top grafting seed changing method which comprises the following steps: S1, cutting a rootstock to form a grafted point, treating a bud ear with a grafting germination liquid, preserving moisture, and cutting; s2, inserting the cut bud ear in the step S1 into a grafted point of the stock, performing bud ear treatment, and sealing the head of the stock to realize grafting; the grafting germination liquid is prepared from the following components: 6-benzylamino adenine, 2, 4-dichlorphenoxyacetic acid and 1-naphthylacetic acid. According to the top grafting seed changing method, the grafting survival rate and the interface healing speed can be effectively increased, and rapid growth of buds and spikes is promoted; wherein the grafting germination liquid composed of 6-benzylamino adenine, 2, 4-dichlorphenoxyacetic acid and 1-naphthylacetic acid is used for treating and subsequently moisturizing the bud ears, so that the bud ears can be prevented from being dehydrated and dried up and losing the healing meristematic ability, in addition, the joint healing time is also remarkably shortened, and the grafting survival rate is improved.

Compositions and methods are provided for delivering hydrophilic functional agents to plants using lipid nanoparticles (NLPs). The NLPs comprise a hydrophilic core surrounded by a lipid shell including at least one sterol, one phospholipid, and one cationic or ionizable lipid. The hydrophilic core enables encapsulation and stabilization of water-soluble agents such as nucleic acids, peptides, small molecules, or plant hormones. The NLPs enhance uptake, protect cargo from degradation, and facilitate delivery to internal plant tissues, including meristems and vascular structures. Methods of treating plants and plant parts with the compositions are also provided.

The invention concerns a method for producing a shoot of a plant comprising germline progenitor cells of a recalcitrant plant. The germline progenitor cells may be modified to comprise a mutation in a sequence of interest. The invention further pertains to plants obtainable by the method of the invention, wherein the plant preferably comprises at least the L2-meristem layer of the recalcitrant plant.

The application belongs to the technical field of genetic engineering, and particularly relates to a negative-sense RNA virus plant genome editing system and application thereof. The genome editing system uses a CRISPR-Cas9 gene editing technology, takes a plant cytoplasmic rhabdovirus as a delivery carrier, is transmitted to a plant by means of a vector, utilizes an RNA movement motif, delivers gene editing components to an axillary meristem, and obtains mutant lateral buds along with plant tillering. The application can complete plant gene editing without tissue culture, overcomes plant tissue culture dependence, expands the range of gene editing lines, and only needs one generation to obtain a pure mutant without going through hybridization and selfing processes.

The invention relates to the technical field of embryo element preparation, in particular to a method for preparing embryo elements at peanut stem end meristem, which comprises the following steps: S1, obtaining a target section of the stem end meristem on a peanut germ in a preset growth period; s2, cleaning and crushing the target section, and mixing with an entrainer in an extraction kettle according to a preset proportion; s3, introducing carbon dioxide at a preset temperature and a preset pressure into the extraction kettle, and completing extraction in a preset time period; s4, sequentially introducing the extracted carbon dioxide into a first-stage separator and a second-stage separator, and carrying out embryo element separation; s5, respectively obtaining a front separation characteristic value and a rear separation characteristic value; s6, preliminarily judging whether the extraction of the embryo element meets the preset standard or not, and determining the reason for not meeting the preset standard; and S7, when the pre-separation characteristic value preliminarily judges that the extraction of the embryo element accords with the preset standard, determining whether the secondary separator performs cyclic extraction or not based on the post-separation characteristic value. The method improves the product quality and stabilizes the extraction efficiency.

The application provides a preparation method of detoxified sweet potato seedlings, comprising the following cultivation steps: washing sweet potatoes, placing the sweet potatoes in a sterilization liquid to accelerate germination, disinfecting the newly born stem tips, placing the stem tips in a culture medium I to culture, and obtaining sweet potato stem tips; when the sweet potato stem tips grow 4-5 leaves, the stem tip meristem is inoculated into a culture medium II, and the culture is carried out to 8-9 stem segments, and sweet potato seedlings are obtained; the sweet potato seedlings are transplanted to a seedling raising tray containing a nutrient medium, and the seedlings are cultured in an artificial climate chamber for 30-35 days, and then the seedlings are transplanted into soil for breeding. The preparation method of the detoxified sweet potato seedlings significantly improves the growth condition of the sweet potato seedlings, increases the plant height, the number of root systems and the length of root systems of the sweet potato seedlings, and significantly improves the net photosynthetic rate (Pn) and the stomatal conductance (Gs) of the detoxified sweet potato seedlings, which is beneficial to the healthy growth of the sweet potato in the seedling stage.

This invention relates to compositions and methods for modifying a Leucine Rich Repeat Receptor Like Protein Kinase (LRR-RLK) gene in plants to increase kernel row number. The invention further relates to plants having increased kemel row number produced using the methods and compositions of the invention.

The invention provides a method for judging flowering morning and evening by using a stem tip development grading stage of brassica napus in a wintering period, and belongs to the technical field of crop growth and development and germplasm resource identification, the method comprises the following steps: starting from the wintering period of the brassica napus, performing field sampling once every 7-15 days to obtain stem tips; the stem tip meristem is exposed, the stem tip is dissected, and a dissecting image is obtained; determining the stem tip diameter and the stem tip meristem included angle as quantitative standards according to the obtained anatomical image; then, according to morphological characteristics and quantitative standards, dividing development stages of the rape stem tips; judging the flowering morning and evening of the rape according to the stem tip development stage: if the stem tip development stage is greater than or equal to 4 during the first field sampling and dissection, judging that the rape is an early flowering germplasm; and if the development stage is less than or equal to 3 during the fourth field sampling and dissection, judging that the germplasm is late florescence germplasm. The problems that in the prior art, the identification period is long, environmental interference is large, and quantitative evaluation is difficult to achieve in the early development stage are solved.