1 results about "Calla" patented technology

The present application relates to a kind of culture medium combination and its method for building color calla regeneration system using tuber, and the culture medium combination includes: callus induction medium, proliferation medium, differentiation medium, wherein, induction medium: with MS as base medium, also contain: agar 6.5-7.0 g / L, sucrose 30 g / L, 6-BA 1.0-2.0 mg / L, NAA 0.5-1.0 mg / L;Or, also contain the following concentration of substance: agar 6.5-7.0 g / L, sucrose 30 g / L, 6-BA 0.5 mg / L, NAA 0.8 mg / L.The present application uses the aseptic budless tuber of color calla grown to specific period as explant, is combined with specific induction medium, proliferation medium and differentiation medium to obtain multiple shoots, and the method is high in callus induction rate, and it provides technical support for color calla tissue culture rapid propagation and the establishment of genetic transformation system.