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30 results about "Calla" patented technology

Calla (bog arum, marsh calla, wild calla, squaw claw, and water-arum) is a genus of flowering plant in the family Araceae, containing the single species Calla palustris.

Tender stem callus plant regeneration induction culture medium for lonicera caerulea

The invention relates to a tender stem callus plant regeneration induction culture medium for lonicera caerulea and solves the problems of low plant differentiation rate and high culture medium cost problem of the auxiliary bud or stem tip culture, which is the main tissue culture of lonicera caerulea. The culture medium consists of a large amount of macroelements, microelements and hormone, wherein the hormone consists of 6-benzyl aminopurine at a concentration of 1.0 to 2.0mg / L and indolebutyric acid or naphthylacetic acid at a concentration of 0.05 to 0.2mg. When the LD1 culture medium of the invention is used, the tender stems of lonicera caerulea can be easily induced to form calla, and the induction rates of the calla are all over 90 percent; and the probability of the induction of the calla into plants is high, the plant differentiation rate is up to 50 to 82 percent which is 66.6 to 173.3 percent higher than that of the conventional Murashige-Skoog (MS) culture medium, and the cost is saved by 45.1 percent compared with the reference MS culture medium.
Owner:NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S

Myxococcus stipitatus preying on plant pathogenic bacteria and application thereof in biological control of bacterial diseases

ActiveCN108559718AGood broad spectrum resistanceGood predation abilityBiocideBacteriaBiotechnologyBacteroides
The invention discloses myxococcus stipitatus preying on plant pathogenic bacteria and application thereof in biological control of bacterial diseases. Slime bacteria BS of antagonistic plant pathogenic bacteria is provided, and the deposit number is CCTCCNO. M2017496. The myxococcus stipitatus BS inhibits the growth of various plant pathogenic bacteria such as cabbage soft rot pathogen, calla soft rot pathogen, tomato bacterial wilt pathogen, potato black smut and pear fire blight by predation, and shows slime bacteria BS broad spectrum resistance to pathogenic bacteria. Pot experiments showthat the slime bacteria BS can effectively inhibit the infection of Pectobacterium carotovorum subsp. carotovorum on potatoes and calla, reduce the incidence of soft rot, and can be used for the biological control of the plant diseases caused by plant pathogenic bacteria.
Owner:NANJING AGRICULTURAL UNIVERSITY

Quick breeding method for color calla high grade blossoming bulb

The invention relates to a method for quickly cultivating the colorful mosque lotus. The invention comprises that cultivating small seed and cultivating the bloom ball, preparing base material, preparing planting bed, planting tube seed, managing, fertilizing, disinfecting, collecting ball, and storing. Compared with present technique, the invention has short cultivation time that reduces one year and high quality, while the root will not etch.
Owner:张璐萍

Method for obtaining haploid plant of calla by anther culture

The invention relates to a method for obtaining the monoploid plant of Zantedeschia aethiopica by anther culture, belonging to the technical field of flower culture. The method comprises the steps that the young bud of Zantedeschia aethiopica is taken out in the bud stage for sterilization and the anther is taken out and placed on the callus inducement culture medium for culturing and inducing the microspores into callus; the callus is put on the bud inducement culture medium for culturing and inducing the callus to form bud; the bud is put on the radication culture medium for culturing and inducing the bud to radicate and the monoploid plant of Zantedeschia aethiopica is obtained. The method is applied to isolated culture of anther of Zantedeschia aethiopica; on average, 2.4 percent of the anther can induce the microspores to form the callus and each callus can differentiate 6.8 buds and the rate of monoploid plant is 69.6 percent. The monoploid plant of Zantedeschia aethiopica obtained by the method needs only one generation to obtain the pure strain with stable heredity by doubling the chromosome, which is an approach for accelerating the purification of breeding materials and process effectively.
Owner:YUNNAN UNIV

Biological preparation capable of preventing and treating rice streak disease and promoting growth and application thereof

InactiveCN101617691AEasy to useEfficient disease controlPlant growth regulatorsBiocideDiseaseCalla
The invention relates to a biological preparation capable of preventing and treating rice streak disease and promoting growth and application thereof, belonging to the technical field of biological pesticide. The biological preparation is prepared by a conventional method, and a producing strain is YFY02 (Lysobacter ginsengisoli). The strain of the biological preparation is characterized in that (1) the colony is round and in a opaque dilute liquid shape, and is dark yellow at initial stage of culture and chocolate brown or dark brown at later stage of culture, and the thallus is in the shape of a long rod; (2) the strain has strong bacteriostatic ability and wide antibacterial spectrum, can keep value on leaf surfaces of crops such as rice and the like and roots of crops such as konjak, calla and the like, can generate substances similar to antibiotics to promote the growth of crops of rice and the like, and has obvious and stable effect on preventing and treating rice streak disease. In the invention, the strain is cultured in liquid or solid and is prepared to liquid preparation or wettable powder. The invention has obvious and stable effect on preventing and treating bacterial diseases of rice streak disease, and good comprehensive properties, and is easy for industrial production.
Owner:YUNNAN AGRICULTURAL UNIVERSITY

Tissue culture quick propagation method for Opisthopappus Shih

The invention discloses a tissue culture quick propagation method for Opisthopappus Shih, which comprises the following: A, a step of obtaining an explant, which is to obtain the explants from aseptic Opisthopappus Shih seedlings; B, a step of induction callus culture, which is to transfer the explants obtained by the step A to an induction callus culture medium and culture the explants till calla is generated; C, a step of propagation subculture, which is to inoculate calla that have a compact structure, fragile and hard texture and irregular spherical or nearly isodiametric cells, grow slowly and are light green onto a propagation subculture medium for subculture; D, a step of rooting culture, which is to transfer the aseptic seedlings which grow to more than 2 centimeters to a rooting culture medium for rooting culture; and E, a step of training and transplanting, which is to train the rooted seedlings at room temperature, transplant the seedlings into a medium and obtain a large number of Opisthopappus Shih seedlings. In the invention, detailed instructions are provided for building an Opisthopappus Shih seedling system, particularly, optimal formulae of the induction callus culture medium, propagation subculture medium and rooting culture medium are provided, the method have high actionability and repeatability and can propagate a large number of Opisthopappus Shih seedlings at one time.
Owner:ZHENGZHOU UNIV

Method for obtaining haploid plant of calla by free microspore culture

The invention relates to a method for culturing and obtaining the monoploid plant of Zantedeschia aethiopica with free microspores, belonging to the technical field of flower breeding. The steps of the method comprise that the Zantedeschia aethiopica stamen with the microspore being at the late nonnuclear stage is fetched for sterilization; the microspore is free mechanically and the cell density is adjusted to 1.0*10<5> microspores / ml with NLN-13% fructose and then the free microspores are pre-cultured in the dark under the temperature of 32 DEG C for two days; and then the microspores are pre-cultured in the dark under the temperature of 25 DEG C for five days and the culture medium is changed for the culture medium of NLN-13% sucrose after culture; continuously the microspores are cultured in the dark under the temperature of 25 DEG C and the microspores are developed into callus; the callus forms the monoploid plant by bud differentiation. The monoploid plant of Zantedeschia aethiopica obtained by the method needs only one generation to obtain the pure strain with stable heredity by doubling the chromosome, which is an approach for accelerating the purification of breeding materials and process effectively.
Owner:YUNNAN UNIV

Konjac cultivating method

The invention discloses a konjac cultivating method. A.muelleri in the Sino-Burmese border area as a female parent and a konjac germplasm A.macrorhizus as a male parent are subjected to intrageneric artificial cross, and seed collecting, close planting and sowing, cultivation and seedball harvesting are performed; a plant is selected from F1 generation, wherein the plant has dark green leaf color, grows robustly, has stems being green stems and having greenish brown stripes, has tender white terminal buds, has white flower type and flower color having uniform light purple spots, has the spathe like calla and shorter than appendant organs, has light yellow-to-white tuber flesh color, and has an atmogenic corm growth pattern comprising that single plants are grown on stems, petiole crotches and main veins and then are subjected to pollination, seed collecting, sowing and screening; then an individual is selected from F2 generation and is used as a new variety, wherein the individual has stems being green stems and having greenish brown stripes, has tender white terminal bud, has white flower type and flower color having uniform light purple spots, has the spathe like calla and shorter than appendant organs, has no pollen produced after flowering, has light yellow-to-white tuber flesh color, has atmogenic corms which can be grown on stems, petiole crotches and main veins, has KGM content more than 70%, and has 39 pairs of chromosomes.
Owner:南京滇缘生物科技有限公司 +1

Television system for calling menu by body posture detection and control method thereof

The invention discloses a television system for calling a menu by body posture detection and a control method thereof. The television system comprises a body posture detection module for detecting body posture signals, a main control module, a storage module for storing posture reference data and a display module, wherein the main control module is electrically connected with the body posture detection module and the storage module respectively to compare the body posture signals with the posture reference data, the main control module is electrically connected with a switching module to calla television menu according to the comparison result and the television menu is displayed via the display module. The menu calling control on the television can be realized only when the body posturedetection module detects the body movement of the user such as gesture, so that the user operation is convenient and fast.
Owner:GUANGDONG CHANGHONG ELECTRONICS

Rule engine-driven data fusion method

ActiveCN110347878AGood current situationSemantic integritySemi-structured data indexingSemi-structured data queryingCallaAlgorithm
The invention belongs to the technical field of data fusion, and discloses a rule engine-driven data fusion method, which comprises the following steps of: setting an expression specification of a fusion rule; setting implementation specifications of the fusion engine; formulating fusion rules of four stages of data preprocessing, data integration, homonymous entity matching and homonymous entityfusion and constructing fusion rule bases corresponding to the four stages; analyzing fusion rules in the four-stage fusion rule library, creating a corresponding fusion engine for each fusion rule, and constructing fusion engine libraries corresponding to the four stages; reading the multi-source heterogeneous data; and enabling a user to specify a fusion rule, create a data fusion task, and calla fusion engine to automatically complete a data fusion process. According to the invention, the problem that a data fusion method in the prior art is difficult to meet the dynamic demand of data issolved.
Owner:CETC BIGDATA RES INST CO LTD +1

High-quality corm of colorful calla and method for cultivating same

The invention discloses a high-quality corm of colorful calla, which has preservation number of CGMCC No.2457. Compared with the corm prepared by the prior art, the corm of colorful calla has far longer storage time; and the corms prepared by the prior art which are planted after being stored for six months do not blossom at the same time, which directly affects the commodity value of pot flowersand cut flowers. The storage time of the high-quality corm of colorful calla of the invention reaches over 10 months, and makes the supply of the corms for producing pot flowers and cut flowers possible all the year.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES

Botanical disinfectant and preparation method thereof

The invention discloses a botanical disinfectant and a preparation method thereof. The botanical disinfectant is prepared from the following raw materials in parts by weight: 10 to 15 parts of celastrus angulatus, 5 to 10 parts of litsea cubeba, 1 to 5 parts of radix saposhnikoviae, 1 to 3 parts of cornu rhinoceri, 5 to 10 parts of sponge gourd, 10 to 15 parts of radix euphorbiae lantu, 10 to 15 parts of calla peel, 5 to 10 parts of radix coptidis, 3 to 8 parts of horsetail beefwood sprout, 5 to 10 parts of fructus forsythiae, 5 to 10 parts of herba solani nigri, 10 to 15 parts of semen ricini, 5 to 10 parts of radix scutellariae and 5 to 10 parts of avocado peel. The botanical disinfectant uses multiple vegetable extracts as main raw materials, can be used for killing various malignant bacteria, viruses and insect pests, meanwhile, is safe and free-pollution to an environment, meanwhile, is used for inhibiting bacterial reproduction, and further, is simple in making process, low-price in the raw material and suitable for large-scale production.
Owner:新昌县奥而特农业科技有限公司

Quick-preparation type plant alkaloid insecticidal powder for peach trees and preparation method of quick-preparation type plant alkaloid insecticidal powder

The invention discloses quick-preparation type plant alkaloid insecticidal powder for peach trees and a preparation method of the quick-preparation type plant alkaloid insecticidal powder. the plant alkaloid insecticidal powder is prepared from the following components in parts by weight: 4.534-8.655 parts of 1,2-dicarboxy-propyl carborane, 3.982-9.376 parts of N-bromosuccinimide, 9.456-14.269 parts of cod-liver oil extracts, 4.257-8.657 parts of methyl methacrylate, 1.354-4.156 parts of biscyclopentadienyl iron, 5.698-7.156 parts of acetic p-toluenesulfonic anhydride, 4.598-7.325 parts of divinyltetramethyldisiloxane, 18.657-20.723 parts of alliin, 10.687-15.893 parts of divinyltetramethyldisiloxane, 12.586-17.951 parts of calla extract liquor, 7.635-12.675 parts of methyl trifluoromethanesulfonate, 1.589-7.896 parts of N, N-dimethylformamide, 10.893-16.753 parts of 2-oxo-1-imidazolidinecarbonyl chloride, 8.394-12.756 parts of 3-acetyl-1-chlorocarbonyl-2-imidazolidone, 14.659-18.546 parts of 3-chlorocarbonyl-1-methanesulfonyl-2-imidazolidinone, 4.893-8.885 parts of 1-(2-hydroxyethyl)-2-imidazolidinone, 6.786-10.563 parts of 1-methylsulphonyl-2-imidazolidone, 15.689-22.486 parts of 1,3-dimethyl-2-imidazolidinone, 17.986-22.258 parts of 1-acetyl-2-imidazolidinone and the like and 5.698-8.467 parts of pistia stratiotes extract liquor.
Owner:徐州市贾汪区信守农业发展有限公司

Medicine combination treating thyroncus and application thereof

InactiveCN105232834AEnlarged folliclesIncreased gel formationEndocrine system disorderPlant ingredientsCallaSenna occidentalis
The invention discloses medicine combination treating thyroncus and application thereof. The combination is composed of active components and pharmaceutical auxiliaries. The active components are prepared from, by weight, 25-33 parts of ficus tikoua, 11-20 parts of hairyvein agrimony, 25-33 parts of ternate buttercup roots, 7-12 parts of red cluster peppers, 4-9 parts of iris uniflora, 4-9 parts of calla palustris, 9-15 parts of eurya patentipila chun, 6-9 parts of senna occidentalis and 6-9 parts of ephedra. The medicine can inhibit hyperplasia of thyroid tissue and promote differentiation of the thyroid tissue, and therefore hard lumps can be removed, and therefore the medicine combination has the functions of inducing diuresis for removing edema and boosting immunity and can be used for treating thyroid enlargement.
Owner:王亚鹤

Method for obtaining haploid plant of calla by free microspore culture

The invention relates to a method for culturing and obtaining the monoploid plant of Zantedeschia aethiopica with free microspores, belonging to the technical field of flower breeding. The steps of the method comprise that the Zantedeschia aethiopica stamen with the microspore being at the late nonnuclear stage is fetched for sterilization; the microspore is free mechanically and the cell densityis adjusted to 1.0*10<5> microspores / ml with NLN-13% fructose and then the free microspores are pre-cultured in the dark under the temperature of 32 DEG C for two days; and then the microspores are pre-cultured in the dark under the temperature of 25 DEG C for five days and the culture medium is changed for the culture medium of NLN-13% sucrose after culture; continuously the microspores are cultured in the dark under the temperature of 25 DEG C and the microspores are developed into callus; the callus forms the monoploid plant by bud differentiation. The monoploid plant of Zantedeschia aethiopica obtained by the method needs only one generation to obtain the pure strain with stable heredity by doubling the chromosome, which is an approach for accelerating the purification of breeding materials and process effectively.
Owner:YUNNAN UNIV

Tender stem callus plant regeneration induction culture medium for lonicera caerulea

The invention relates to a tender stem callus plant regeneration induction culture medium for lonicera caerulea and solves the problems of low plant differentiation rate and high culture medium cost problem of the auxiliary bud or stem tip culture, which is the main tissue culture of lonicera caerulea. The culture medium consists of a large amount of macroelements, microelements and hormone, wherein the hormone consists of 6-benzyl aminopurine at a concentration of 1.0 to 2.0mg / L and indolebutyric acid or naphthylacetic acid at a concentration of 0.05 to 0.2mg. When the LD1 culture medium of the invention is used, the tender stems of lonicera caerulea can be easily induced to form calla, and the induction rates of the calla are all over 90 percent; and the probability of the induction of the calla into plants is high, the plant differentiation rate is up to 50 to 82 percent which is 66.6 to 173.3 percent higher than that of the conventional Murashige-Skoog (MS) culture medium, and thecost is saved by 45.1 percent compared with the reference MS culture medium.
Owner:NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S

Biological preparation capable of preventing and treating rice streak disease and promoting growth and application thereof

The invention relates to a biological preparation capable of preventing and treating rice streak disease and promoting growth and application thereof, belonging to the technical field of biological pesticide. The biological preparation is prepared by a conventional method, and a producing strain is YFY02 (Lysobacter ginsengisoli). The strain of the biological preparation is characterized in that (1) the colony is round and in a opaque dilute liquid shape, and is dark yellow at initial stage of culture and chocolate brown or dark brown at later stage of culture, and the thallus is in the shapeof a long rod; (2) the strain has strong bacteriostatic ability and wide antibacterial spectrum, can keep value on leaf surfaces of crops such as rice and the like and roots of crops such as konjak, calla and the like, can generate substances similar to antibiotics to promote the growth of crops of rice and the like, and has obvious and stable effect on preventing and treating rice streak disease. In the invention, the strain is cultured in liquid or solid and is prepared to liquid preparation or wettable powder. The invention has obvious and stable effect on preventing and treating bacterialdiseases of rice streak disease, and good comprehensive properties, and is easy for industrial production.
Owner:YUNNAN AGRICULTURAL UNIVERSITY

Online car-hailing hand-raising taxi-calling system

The invention discloses an online car-hailing hand-raising taxi-calling system, and relates to the technical field of online car hailing. The system comprises a customer module, an online car-hailingdriver module and a driver passenger hotel merchant designated driving module which are parallel to one another; the customer module comprises a customer scene module, a customer scanning binding module and a customer terminal module, the customer scene module is connected with the customer scanning binding module, and the customer scanning binding module is connected with the customer terminal module; the online car-hailing driver module comprises a driver scene module, a driver scanning binding module and a driver terminal module, the driver scene module is connected with the driver scanningbinding module, and the driver scanning binding module is connected with the driver terminal module. The online car-hailing hand-raising taxi-calling system can enable the user to raise hands to calla taxi, realize self-payment and automatic payment and charging, and is convenient to use.
Owner:何星龙

Quick breeding method for color calla high grade blossoming bulb

The invention relates to a method for quickly cultivating the colorful mosque lotus. The invention comprises that cultivating small seed and cultivating the bloom ball, preparing base material, preparing planting bed, planting tube seed, managing, fertilizing, disinfecting, collecting ball, and storing. Compared with present technique, the invention has short cultivation time that reduces one year and high quality, while the root will not etch.
Owner:张璐萍

UV-spectrophotometric determination of polysaccharide content in calla fern

The invention discloses an ultraviolet spectrophotometric method for determining the polysaccharide content in calla fern medicinal materials. Using anhydrous glucose as a contrast, the ultraviolet spectrophotometer is used to measure the polysaccharide content in calla fern polysaccharide extract. The invention perfects the quality detection standard of the calla fern medicinal material, and lays a foundation for the establishment of the calla fern medicinal material quality standard; moreover, the method has high sensitivity, simple and convenient operation, and accurate results; popularization and application of the present invention can ensure the quality of the calla fern medicinal material, To ensure the safety, effectiveness and stability of its clinical medication.
Owner:GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI

Hand cream with flavor of common callalily

InactiveCN107714599ASimple processFresh and long-lasting aromaCosmetic preparationsToilet preparationsAcetic acidCalla
The invention relates to the technical field of skin care products, in particular to a calla lily scented hand cream. This calla lily scented hand cream contains 12g of glucose, 0.8g of honey, 0.11g of rose water, 0.8g of Na‑PCA, 0.13g of hyaluronic acid, and 0.25g of guaiacyl acetate. This calla lily-scented hand cream is simple to craft and has a fresh, long-lasting scent.
Owner:周本武

Ant-rejection liquid for different fruits on one tree

The invention relates to an anti-rejection liquid for different fruits on one tree. The anti-rejection liquid comprises, by mass, 5-15 g of narcissus bulb juice, 5-15 g of common calla tuber juice, 3-6 g of verbena leaf juice, 60-100 million units of penicillin, 0.5-1 mg of dexamethasone tablets and 60-90 g of distilled water. The anti-rejection liquid is simple in components, convenient for preparation and low in cost, can effectively solve rejection generated by grafting different fruit trees on one fruit tree, increases grafting survival rate and realizes that one tree produces a variety of fruits.
Owner:居炳生

Skincare preparation for rapidly treating angiotelectasis

A skin care preparation for rapidly treating telangiectasia, wherein the preparation raw materials include: 7-15 parts of rhododendron extract, 4-10 parts of calla lily extract, 15-30 parts of sansevieria juice, and white amaranth 10-20 parts of syrup, 15-25 parts of chrysanthemum, 40-50 parts of ethanol, 0.1-1 part of surfactant, and the rest is water. The skin care preparation obtained through the scientific ratio of ethanol, sansevieria juice, white amaranth juice and chrysanthemum can quickly treat telangiectasia, and there is no pain in the process of removing capsaicin, and the product can be used for many times The use saves unnecessary waste and has better economic benefits.
Owner:阮品伟

Tissue culture quick propagation method for Opisthopappus Shih

The invention discloses a tissue culture quick propagation method for Opisthopappus Shih, which comprises the following: A, a step of obtaining an explant, which is to obtain the explants from aseptic Opisthopappus Shih seedlings; B, a step of induction callus culture, which is to transfer the explants obtained by the step A to an induction callus culture medium and culture the explants till calla is generated; C, a step of propagation subculture, which is to inoculate calla that have a compact structure, fragile and hard texture and irregular spherical or nearly isodiametric cells, grow slowly and are light green onto a propagation subculture medium for subculture; D, a step of rooting culture, which is to transfer the aseptic seedlings which grow to more than 2 centimeters to a rooting culture medium for rooting culture; and E, a step of training and transplanting, which is to train the rooted seedlings at room temperature, transplant the seedlings into a medium and obtain a large number of Opisthopappus Shih seedlings. In the invention, detailed instructions are provided for building an Opisthopappus Shih seedling system, particularly, optimal formulae of the induction callus culture medium, propagation subculture medium and rooting culture medium are provided, the method have high actionability and repeatability and can propagate a large number of Opisthopappus Shih seedlings at one time.
Owner:ZHENGZHOU UNIV

A kind of cultivation method of konjac

ActiveCN106561444BSolving hybrid breeding problemsPlant genotype modificationCallaGermplasm
The invention discloses a konjac cultivating method. A.muelleri in the Sino-Burmese border area as a female parent and a konjac germplasm A.macrorhizus as a male parent are subjected to intrageneric artificial cross, and seed collecting, close planting and sowing, cultivation and seedball harvesting are performed; a plant is selected from F1 generation, wherein the plant has dark green leaf color, grows robustly, has stems being green stems and having greenish brown stripes, has tender white terminal buds, has white flower type and flower color having uniform light purple spots, has the spathe like calla and shorter than appendant organs, has light yellow-to-white tuber flesh color, and has an atmogenic corm growth pattern comprising that single plants are grown on stems, petiole crotches and main veins and then are subjected to pollination, seed collecting, sowing and screening; then an individual is selected from F2 generation and is used as a new variety, wherein the individual has stems being green stems and having greenish brown stripes, has tender white terminal bud, has white flower type and flower color having uniform light purple spots, has the spathe like calla and shorter than appendant organs, has no pollen produced after flowering, has light yellow-to-white tuber flesh color, has atmogenic corms which can be grown on stems, petiole crotches and main veins, has KGM content more than 70%, and has 39 pairs of chromosomes.
Owner:南京滇缘生物科技有限公司 +1

Calla high yield cultivation method

The high-yield calla cultivating method includes forming cultivating furrows of 1-1.5 m width, 20-40 m length and 30-50 cm width inside green house, digging irrigation ditch of 30-50 cm width and 30-50 cm depth, keeping water depth of 15-35 cm and water pH 6.5-7 inside the irrigation ditch, applying composite organic base fertilizer with equal ratio of nitrogen fertilizer, phosphate fertilizer and potash fertilizer, planting calla bulb, maintaining the relative humidity inside the green house in 80-90 % and conventional field management. Compared with available technology, the present invention can bloom calla all the year around, has one time higher yield, and is simple, practical and low in cost. The said method is suitable for large scale cultivation of calla.
Owner:GUIZHOU ACAD OF SCI

Method for obtaining haploid plant of calla by anther culture

The invention relates to a method for obtaining the monoploid plant of Zantedeschia aethiopica by anther culture, belonging to the technical field of flower culture. The method comprises the steps that the young bud of Zantedeschia aethiopica is taken out in the bud stage for sterilization and the anther is taken out and placed on the callus inducement culture medium for culturing and inducing the microspores into callus; the callus is put on the bud inducement culture medium for culturing and inducing the callus to form bud; the bud is put on the radication culture medium for culturing and inducing the bud to radicate and the monoploid plant of Zantedeschia aethiopica is obtained. The method is applied to isolated culture of anther of Zantedeschia aethiopica; on average, 2.4 percent of the anther can induce the microspores to form the callus and each callus can differentiate 6.8 buds and the rate of monoploid plant is 69.6 percent. The monoploid plant of Zantedeschia aethiopica obtained by the method needs only one generation to obtain the pure strain with stable heredity by doubling the chromosome, which is an approach for accelerating the purification of breeding materials and process effectively.
Owner:YUNNAN UNIV
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