30 results about "Calla" patented technology

The invention relates to a method for culturing and obtaining the monoploid plant of Zantedeschia aethiopica with free microspores, belonging to the technical field of flower breeding. The steps of the method comprise that the Zantedeschia aethiopica stamen with the microspore being at the late nonnuclear stage is fetched for sterilization; the microspore is free mechanically and the cell densityis adjusted to 1.0*10<5> microspores / ml with NLN-13% fructose and then the free microspores are pre-cultured in the dark under the temperature of 32 DEG C for two days; and then the microspores are pre-cultured in the dark under the temperature of 25 DEG C for five days and the culture medium is changed for the culture medium of NLN-13% sucrose after culture; continuously the microspores are cultured in the dark under the temperature of 25 DEG C and the microspores are developed into callus; the callus forms the monoploid plant by bud differentiation. The monoploid plant of Zantedeschia aethiopica obtained by the method needs only one generation to obtain the pure strain with stable heredity by doubling the chromosome, which is an approach for accelerating the purification of breeding materials and process effectively.

The invention relates to a biological preparation capable of preventing and treating rice streak disease and promoting growth and application thereof, belonging to the technical field of biological pesticide. The biological preparation is prepared by a conventional method, and a producing strain is YFY02 (Lysobacter ginsengisoli). The strain of the biological preparation is characterized in that (1) the colony is round and in a opaque dilute liquid shape, and is dark yellow at initial stage of culture and chocolate brown or dark brown at later stage of culture, and the thallus is in the shapeof a long rod; (2) the strain has strong bacteriostatic ability and wide antibacterial spectrum, can keep value on leaf surfaces of crops such as rice and the like and roots of crops such as konjak, calla and the like, can generate substances similar to antibiotics to promote the growth of crops of rice and the like, and has obvious and stable effect on preventing and treating rice streak disease. In the invention, the strain is cultured in liquid or solid and is prepared to liquid preparation or wettable powder. The invention has obvious and stable effect on preventing and treating bacterialdiseases of rice streak disease, and good comprehensive properties, and is easy for industrial production.

The invention discloses an alocasia macrorrhizos test-tube plantlet multiplication method which involves MS culture media, a hormone, a carbon source, a cultivation mode and cultivation time. According to the method, alocasia macrorrhizos test-tube plantlets are used as a material, the induction and multiplication effect is obvious, the growth coefficient reaches more than eight, and the alocasia macrorrhizos industrialized production speed can be greatly increased. By the adoption of the method, the problems that a traditional alocasia macrorrhizos division propagation mode is low in propagation coefficient and long in period and can be limited by the environmental factors such as seasons are solved, a liquid cultivation mode is adopted, agar or carrageenan does not need to be added, the tissue culture operation process is reduced, the tissue culture cost can be lowered, the cultivation environment is uniform, nutrient uptake can be promoted, and alocasia macrorrhizos seedlings generated in the liquid cultivation mode are more orderly than aseptic seedlings generated through callus tissue.

The invention discloses a freenhouse accelerated culture method for colored zantedeschia in the northwest region. The colored zantedeschia grows rapidly in a greenhouse with a relatively low temperature or without a heating condition through technologies of constructing facilities, disinfecting seed balls, accelerating germination, potting, promoting plant growth, dwarfing plants and the like, and lodging is resisted, so that the colored zantedeschia has a relatively good ornamental value, a planting range of the colored zantedeschia is expanded, and the economic benefits are improved.

The invention relates to a method for quickly cultivating the colorful mosque lotus. The invention comprises that cultivating small seed and cultivating the bloom ball, preparing base material, preparing planting bed, planting tube seed, managing, fertilizing, disinfecting, collecting ball, and storing. Compared with present technique, the invention has short cultivation time that reduces one year and high quality, while the root will not etch.

The invention discloses a tissue culture quick propagation method for Opisthopappus Shih, which comprises the following: A, a step of obtaining an explant, which is to obtain the explants from aseptic Opisthopappus Shih seedlings; B, a step of induction callus culture, which is to transfer the explants obtained by the step A to an induction callus culture medium and culture the explants till calla is generated; C, a step of propagation subculture, which is to inoculate calla that have a compact structure, fragile and hard texture and irregular spherical or nearly isodiametric cells, grow slowly and are light green onto a propagation subculture medium for subculture; D, a step of rooting culture, which is to transfer the aseptic seedlings which grow to more than 2 centimeters to a rooting culture medium for rooting culture; and E, a step of training and transplanting, which is to train the rooted seedlings at room temperature, transplant the seedlings into a medium and obtain a large number of Opisthopappus Shih seedlings. In the invention, detailed instructions are provided for building an Opisthopappus Shih seedling system, particularly, optimal formulae of the induction callus culture medium, propagation subculture medium and rooting culture medium are provided, the method have high actionability and repeatability and can propagate a large number of Opisthopappus Shih seedlings at one time.

The invention discloses a konjac cultivating method. A.muelleri in the Sino-Burmese border area as a female parent and a konjac germplasm A.macrorhizus as a male parent are subjected to intrageneric artificial cross, and seed collecting, close planting and sowing, cultivation and seedball harvesting are performed; a plant is selected from F1 generation, wherein the plant has dark green leaf color, grows robustly, has stems being green stems and having greenish brown stripes, has tender white terminal buds, has white flower type and flower color having uniform light purple spots, has the spathe like calla and shorter than appendant organs, has light yellow-to-white tuber flesh color, and has an atmogenic corm growth pattern comprising that single plants are grown on stems, petiole crotches and main veins and then are subjected to pollination, seed collecting, sowing and screening; then an individual is selected from F2 generation and is used as a new variety, wherein the individual has stems being green stems and having greenish brown stripes, has tender white terminal bud, has white flower type and flower color having uniform light purple spots, has the spathe like calla and shorter than appendant organs, has no pollen produced after flowering, has light yellow-to-white tuber flesh color, has atmogenic corms which can be grown on stems, petiole crotches and main veins, has KGM content more than 70%, and has 39 pairs of chromosomes.

The invention relates to a method for obtaining the monoploid plant of Zantedeschia aethiopica by anther culture, belonging to the technical field of flower culture. The method comprises the steps that the young bud of Zantedeschia aethiopica is taken out in the bud stage for sterilization and the anther is taken out and placed on the callus inducement culture medium for culturing and inducing the microspores into callus; the callus is put on the bud inducement culture medium for culturing and inducing the callus to form bud; the bud is put on the radication culture medium for culturing and inducing the bud to radicate and the monoploid plant of Zantedeschia aethiopica is obtained. The method is applied to isolated culture of anther of Zantedeschia aethiopica; on average, 2.4 percent of the anther can induce the microspores to form the callus and each callus can differentiate 6.8 buds and the rate of monoploid plant is 69.6 percent. The monoploid plant of Zantedeschia aethiopica obtained by the method needs only one generation to obtain the pure strain with stable heredity by doubling the chromosome, which is an approach for accelerating the purification of breeding materials and process effectively.