35 results about "Cattleya" patented technology

The invention relates to a vegetative propagation method for cattleya, in particular to a vegetative propagation method for butterfly orchid. The vegetative propagation method for butterfly orchid, provided by the invention, comprises the following steps of: by taking pedicel and axillary bud of butterfly orchid as explant, inoculating the explant into a culture medium for culturing; and performing subculture, rooting culture and transplanting culture on the induced new buds in turn. According to the method provided by the invention, the tillering of the butterfly orchid is realized in an artificially prepared culture medium; the single-stalk reproduction feature of the butterfly orchid is broken through; an induced pedicel seedling can be obtained without hurting a butterfly orchid stock plant according to the method; and the pedicel of the same stock plant can be repeatedly used. The seedlings produced according to the method are robust, trim and consistent; the source problem of excellent butterfly orchid seedlings during production is solved; and more economic and social benefits can be created.

The invention discloses a cattleya florescence regulating and controlling method. The selected cattleya plants are put into a greenhouse with a day temperature of 25 DEG C and a night temperature of 20 DEG C, photoperiod is set with a light period of 12h and a night period of 12h, a light intensity of 320 mu mol X m-2 X s-1 and an air humidity of 70%-80%, and the process continues for six weeks. High P fertilizer with a N:P:K ratio of 10:30:20 is applied for forcing the cattleya, and the plants are sprayed once each week by monopotassium phosphate aqueous solution diluted by 1000 times for improving cold resisting ability and in-vivo nutrient accumulation of the plants, and further improving the flowering. From September, the high P fertilizer is applied for forcing the cattleya, the cattleya is fertilized once after watering until the florescence is stopped. Therefore, the cattleya is abloom in time before holidays such as National Day, Spring Festival and the like, and the commodity value of the cattleya can be increased.

The invention discloses a method for screening tulasnella fungi and establishing a symbiotic system for promoting seed germination of cymbidium mannii and cattleya hybrida. A Tcs1 bacterial strain isadopted and establishes a symbiotic relation together with one or more orchid seeds including cymbidium mannii seeds, cattleya hybrid YK seeds and cattleya hybrid YD seeds to promote germination of orchid seeds. A symbiotic germination test is carried out on an artificial medium through adoption of tulasnella fungi and orchid seeds, and is compared with a control group of orchid seeds with no fungi inoculated to screen mycorrhizal fungi capable of widely efficiently promoting seed germination and to screen appropriate symbiotic mediums. Efficient symbiotic fungi for a germination stage of orchid seeds are obtained, the germination rate of orchid seeds is improved via symbiotic germination culture technique, and a symbiotic system of orchid and mycorrhizal fungi is established.

The invention provides a rapid breeding method of Cattleya by improving the current flow of production, the method comprises fluid culturing on earlier stage, so as to promote the seed to grow fast, then directly inoculating the original bulbodium to the sprout culture medium for sprout production in one step. The method can reduce culture cost and time greatly, and can be applied to Catteva genus, near source bigeneric hybid seedling production and breeding works.

The present invention relates to the preparation of plant cell cultures belonging to the Dendrobium Phalaenopsis, Anisellia, Polyrrhiza, Vanilla, Cattleya and Vanda genera and their use in cosmetic, nutritional and pharmaceutical fields. In particular, the present invention regards a selected cell culture of meristematic cells of plants belonging to the Dendrobium Phalaenopsis, Anisellia, Polyrrhiza, Vanilla, Cattleya and Vanda genera characterized in that it contains an amount of phenylpropanoids greater than 0.1% expressed on the cell dry weight and an amount of hydrosoluble polysaccharides greater than 5% expressed on the cell dry weight, as well as preparations, pharmaceutical and cosmetic compositions or food supplements containing said cell culture.

The invention discloses a breeding method for vanda. The breeding method comprises the following steps of: selecting a full terminal bud which is germinated in the current year as a material; removing redundant leaves for induction culture; germinating strong buds after 15-20 days; carrying out enrichment culture on the buds for 20-30 days; and performing root induction on the buds for 20-25 days to obtain seedlings for transplanting, wherein the survival rate is higher than 95%. The expanding propagation coefficient is high, and nursery-grown plants grow well after surviving. The vanda is single-axis stem cattleya, so that lateral buds are almost not generated. Moreover, stem tips contain many polyphenols with strong activity, and the cut stem tips are easy to brown during the culture, so that the culture is difficult to succeed; and natural sexual propagation is difficult to succeed as seed germination depends on symbiotic bacteria nourishing. The technical scheme provided by the invention solves the problems of low survival rate, slow propagation and the like in the prior art, and has remarkable economic and social benefits.

The invention relates to the technical field of plant culture, in particular to a rapid culture and propagation method for Cattleya, comprising the following steps: material selection, explant pretreatment, explant disinfection, induction culture, ultrasonic treatment, proliferation culture, rooting culture, strong Seedling cultivation and transplanting. Cattleya can be rapidly cultured and propagated by the method, and the efficiency is high, and the cultivated Cattleya seedlings have high seedling rate, good seedling quality and strong adaptability after cultivation. The demand for commercial production provides technical support for the scale and industrialization of Cattelan. At the same time, the tissue culture propagation method has good disinfection effect and low browning rate, thereby greatly improving the seedling rate and work efficiency, and saving resources.

The invention relates to the technical field of plant tissue culture, in particular to a Cattleya tissue culture propagation method, comprising the following steps: material selection, explant pretreatment, explant disinfection, induction culture, proliferation culture, strong seedling rooting and transplanting , Cattleya can be rapidly cultivated and propagated through the present invention, and the cultivated Cattleya seedlings have a high seedling rate, good quality, and strong adaptability after cultivation, and meet the requirements of commercialization in a short period of time by means of biotechnology. The demand for production provides technical support for the large-scale and industrialized cultivation of Cattelan. At the same time, the tissue culture propagation method has good disinfection effect and low browning rate, thereby greatly improving the seedling rate and work efficiency, and saving resources.

The invention discloses a wide-spectrum sterile sowing culture medium II for tropical orchids. Each L of culture medium contains the following components by weight: 120g of NH4O3, 34g of KNO3, 440g of CaCl2.2H2O, 370g of MgSO4.7H2O, 90g of KH2PO4, 20g of saccharose, 2g of activated carbon, 5.8g of agar and the balance of 1 / 4MS microelements, organic components and iron salt; and the pH value of the culture medium is 5.7. The tropical orchids are cultured by the following steps: carrying culturing in a mature fruit pod period; and picking un-cracked fruit pods when fruit stems of the fruit pods starting to yellow, wherein the culture temperature is 24-26 DEG C, the illumination intensity is 60 micro mol.m / 2s<-1>, and the illumination time is 12 hour / day. By utilizing the culture medium, the germination rate of paphiopedilum seeds is above 70%, the germination rates of cattleya, butterfly orchid and dendrobium seeds are above 90%, and the germination rates of other tropical orchid seeds are above 80%.

The invention discloses a Cattleya pollen storage method, which includes dry storage of Cattleya pollen at 4 DEG C and below or humid storage at -20 DEG C, preferably, dry storage at -20 DEG C. By the method, vitality of the Cattleya pollen can be maintained for more than 2 years, pollination effect is unaffected, and breeding is available all year round.

The invention discloses R2R3 MYB transcription factors promoting anthocyanin formation in two orchids. The transcription factors are shown in Figure 1 and Figure 2, and the transcription factors are isolated and identified from Cattleya. A transgenic material library of orchid ploants with a red flower trait can be enriched through corresponding transcription factors, and strong support for molecular breeding of orchids with the red flower trait in the future is provided.

The invention discloses an aseptic seeding medium for broad-spectrum tropical orchids; each L of the medium comprises 2 g of Hyponex No. 1, 1 g of tryptone, 20 g of banana, 100 g of mashed potato, 10 g of sucrose, 2 g of activated carbon, and 5.8 pg of agar, under pH of 5.7. Culturing is performed in the stage of mature capsules; to be specific, non-cracked capsules are picked from the time when stems of the capsules start to yellow. Culturing temperature is 24-26 DEG C, light intensity is 60 Mumol.m<-2>s<-1>, and illumination time is 12 h / d. After the use of the medium, germination rate of Paphiopedilum seeds is 70% and above, germination rate of Cattleya, Phalaenopsis and Dendrobium seeds is 90% and above, and germination rate of seeds of other tropical orchid is 80% and above.

The invention discloses Cattleya pollen germination medium, which is liquid medium comprising cane sugar, H3BO3 and CaCl2. Preferably, concentrations of the cane sugar, the H3BO3 and the CaCl2 are respectively 100g / L-1, 40mg / L-1 and 150mg / L-1. By using the Cattleya pollen germination medium, germination of pollen can be observed after 3 hours, pollen mass completely dissolves in the liquid after 24 hours, germination of the pollen can be observed vividly, and efficiency can be improved greatly.

The invention discloses a primary cattleya hybrida inducing medium and an explant pretreatment method. The primary cattleya hybrida inducing medium takes an improved MS culture medium as a basic culture medium; citric acid, salicylic acid, lanthanum nitrate, 6-BA, NAA, agar powder, cane sugar and silica gel are added into the basic culture medium; the browning of the cattleya hybrida explants can be effectively alleviated.

The invention provides a flowering phase maintenance method for cattleya hybrida. The method comprises the following steps that (1) in spring maintenance, the indoor overwintering cattleya hybrida is moved out of a room when the temperature is higher than 19 DEG C, and during the sprouting period, the cattleya hybrida is watered once every morning and is subjected to topdressing every other 12 days; when the temperature is stably higher than 16 DEG C, the cattleya hybrida is repotted, and after the repotted cattleya hybrida grows new roots, the cattleya hybrida is subjected to topdressing three times every month; (2) in summer maintenance, the light shading percentage is adjusted to be 55%-70%; watering is conducted once every morning after a matrix is dried; fertilizer is applied once every week; the day temperature is adjusted to be 25-35 DEG C, and the night temperature is adjusted to be 15-20 DEG C; (3) in autumn maintenance, the light shading percentage is adjusted to be 35%-50% in early autumn, and light shading facilities are removed at the end of October; fertilizer is applied once every week, and fertilizer application is stopped after flower buds are formed; (4) in winter maintenance, when the temperature is lower than 12 DEG C, the cattleya hybrida is moved indoors and is watered every other two days; (5) fertilizer and water management is conducted; (6) chemical maintenance is conducted. The flowering phase maintenance method can effectively improve the flowering stability and the flowering percentage of the cattleya hybrida, improve the ornamental value and facilitate popularization.

The present invention disclose a regulating cattleya bloom during the new year's day and the spring festival, proceeding low-temperature control in the bud sheath-breaking phase of the cattleya, the daytime average temperature is kept at 10 DEG, and 6 DEG at night, which can delay the florescence of the cattleya; proceeding heating treatment one week before the new year's day or the spring festival, the day temperature is kept at 23 DEG and the 20 DEG at night to enable the cattleya bloom during the new year's day and the spring festival without any influence to the flowering quality, and the flowering regularity is more than 95%, so that increasing commodity value of the cattleya and implementing commercial production of the cattleya.

The invention provides a cattleya SSR molecular marker primer composition, a fingerprint and application thereof in identification of cattleya germ plasm resources and belongs to the technical field of molecular biology. The invention provides 15 SSR molecular markers and an amplification primer composition thereof of cattleya, 26 different germ plasm resources of the cattleya are subjected to genetic diversity analyzing and molecule identity constructing by using a TP-M13-SSR technology, germ plasm resources of the cattleya can be identified accurately, efficiently and stably, and thus, a foundation is settled for germ plasm resource identification, affinity relationship analysis, trait gene locating, molecular-marker-assisted breeding, cattleya-related molecular researches, and the like of the cattleya.

The invention discloses a method for screening tulasnella fungi and establishing a symbiotic system for promoting seed germination of cymbidium mannii and cattleya hybrida. A Tcs1 bacterial strain isadopted and establishes a symbiotic relation together with one or more orchid seeds including cymbidium mannii seeds, cattleya hybrid YK seeds and cattleya hybrid YD seeds to promote germination of orchid seeds. A symbiotic germination test is carried out on an artificial medium through adoption of tulasnella fungi and orchid seeds, and is compared with a control group of orchid seeds with no fungi inoculated to screen mycorrhizal fungi capable of widely efficiently promoting seed germination and to screen appropriate symbiotic mediums. Efficient symbiotic fungi for a germination stage of orchid seeds are obtained, the germination rate of orchid seeds is improved via symbiotic germination culture technique, and a symbiotic system of orchid and mycorrhizal fungi is established.

The invention provides the rapid propagation technology of Cattleya seedlings. Cattleya is known as the king of orchids, with beautiful flower shapes, bright colors and high ornamental value. The trade volume in the international market is huge, and it may also become a new tropical orchid sales hotspot after Phalaenopsis and Whelan orchid in the Chinese market. However, the existing conventional production technology adopts solid medium for seeding, which takes a long time to germinate and grows slowly; the operation is labor-intensive, the cultivation time is long, and the cost is high. The purpose of the present invention is to improve the production process and use liquid culture in the early stage to promote the rapid germination and growth of seeds, and then directly inoculate the protocorms on the seedling medium to form seedlings in one step, reducing intermediate links, greatly reducing costs and cultivation time, The technology can be applied to the seedling production and breeding of hybrids within the genus Cattleya and close genera, and has a good application prospect.

The invention discloses a cattleya florescence regulating and controlling method. The selected cattleya plants are put into a greenhouse with a day temperature of 25 DEG C and a night temperature of 20 DEG C, photoperiod is set with a light period of 12h and a night period of 12h, a light intensity of 320[mu]mol.m<-2>.s<-1> and an air humidity of 70%-80%, and the process continues for six weeks. High P fertilizer with a N:P:K ratio of 10:30:20 is applied for forcing the cattleya, and the plants are sprayed once each week by monopotassium phosphate aqueous solution diluted by 1000 times for improving cold resisting ability and in-vivo nutrient accumulation of the plants, and further improving the flowering. From September, the high P fertilizer is applied for forcing the cattleya, the cattleya is fertilized once after watering until the florescence is stopped. Therefore, the cattleya is abloom in time before holidays such as National Day, Spring Festival and the like, and the commodityvalue of the cattleya can be increased.

The invention discloses a method for realizing in-vitro preservation of cattleya germplasm resources and recovering the growth after the preservation. The method comprises the following steps: stripping a stem tip from a new growing pseudobulb, putting the stem tip on a surface of a protocorm induction culture medium and culturing to obtain a protocorm; carrying out repeated cutting and subculture on the protocorm, so as to reproduce the protocorms with the needed quantity; culturing by adopting an in-vitro preservation culture medium; after the protocorms enter a development and growth phase, culturing in a dark place at 0 to 5 DEG C; transferring the protocorms which are cultured in the dark place into a differentiation culture medium; differentiating the protocorms to generate seedlings. According to the method disclosed by the invention, a cattleya stem tip culture technology is utilized to induce the protocorms and the protocorms are differentiated to obtain the cattleya seedlings. The protocorms are preserved at low temperature and a proper amount of paclobutrazol is added, so that the preservation time can be greatly prolonged and the number of times of subculture is reduced; the hereditary properties of the germplasm resources are effectively kept. After the protocorms are preserved for certain time, the protocorms can be cultured at normal temprature and under illumination conditions, and the growth of the protocorms can be rapidly recovered and the seedlings are differentiated.

The invention discloses a breeding method for vanda. The breeding method comprises the following steps of: selecting a full terminal bud which is germinated in the current year as a material; removing redundant leaves for induction culture; germinating strong buds after 15-20 days; carrying out enrichment culture on the buds for 20-30 days; and performing root induction on the buds for 20-25 days to obtain seedlings for transplanting, wherein the survival rate is higher than 95%. The expanding propagation coefficient is high, and nursery-grown plants grow well after surviving. The vanda is single-axis stem cattleya, so that lateral buds are almost not generated. Moreover, stem tips contain many polyphenols with strong activity, and the cut stem tips are easy to brown during the culture, so that the culture is difficult to succeed; and natural sexual propagation is difficult to succeed as seed germination depends on symbiotic bacteria nourishing. The technical scheme provided by the invention solves the problems of low survival rate, slow propagation and the like in the prior art, and has remarkable economic and social benefits.

The invention discloses a primary cattleya hybrida inducing medium and an explant pretreatment method. The primary cattleya hybrida inducing medium takes an improved MS culture medium as a basic culture medium; citric acid, salicylic acid, lanthanum nitrate, 6-BA, NAA, agar powder, cane sugar and silica gel are added into the basic culture medium; the browning of the cattleya hybrida explants can be effectively alleviated.

The invention belongs to the field of plant tissue culture, and discloses a method for tissue culture and rapid propagation of Dendrobium nobile variety 'Huoniao'. The method mainly comprises the following steps: 1, disinfecting current-year-grown stems in spring, used as an explant, in NaClO and HgCl2; 2, grafting the disinfected explant to an induction medium MS + 6-BA 3 mg.L<-1> + NAA 0.2 mg.L<-1>, and culturing the explant; 3, transferring the explant to a multiplying medium MS +TDZ 0.1 mg.L<-1> + NAA 0.2 mg.L<-1> + 200 g.L<-1> mashed potato + 1 g.L<-1> active carbon, and culturing the explant; 4, transferring the explant to a seedling strengthening and rooting medium 1 / 2MS + 0.5 mg.L<-1> IBA + 200 g.L<-1> mashed potato, and culturing the explant; and 5, hardening seedlings, and transplanting the seedlings to obtain Dendrobium nobile variety 'Huoniao' tissue culture seedlings. The method for tissue culture and rapid propagation of 'Huoniao' realizes propagation coefficient maximization in a shortest time, and is in favor of rapidly growing seedlings of the cattleya dendrobium variety 'Huoniao', shortening the seedling stage and the growing period in order to reach the purposes of rapid propagation speed, production cost saving and suitableness for large-scale seedling growth. The method has the advantages of extremely small amount of a used plant material, fast growth, short period, strong repeatability, and realization of annual test or production.

The invention provides a Cattleya SSR molecular marker primer composition, a fingerprint code and its application in the identification of Cattleya germplasm resources, belonging to the technical field of molecular biology. The present invention provides 15 SSR molecular markers of Cattleya and their amplification primer compositions, and utilizes TP-M13-SSR technology to analyze the genetic diversity of 26 different germplasm resources within the genus and construct molecular ID cards , can accurately, efficiently and stably identify the germplasm resources of Cattleya, and lay a solid foundation for the identification of germplasm resources of Cattleya, analysis of kinship, mapping of trait genes, molecular marker-assisted breeding and related molecular research of Cattleya. Base.

The invention provides a Chinese mugwort essence oil perineum stick and a production process and application thereof. The Chinese mugwort essence oil perineum stick comprises an inner Chinese mugwort essence oil herb layer and an outer breathable cotton layer, a stickup layer is arranged on the bottom layer of the breathable cotton layer, and the Chinese mugwort essence oil herb layer mainly comprises, by weight, 3-5 parts of Chinese mugwort essence oil, 15-20 parts of mulberry twigs, 2-5 parts of rhizome of rehmannia, 1-2 parts of rheum officinale, 2-6 parts of cattleya, 1-2 parts of mastic, 2-4 parts of radix angelicae tuhuo, 1-3 parts of cortex phellodendri, 1-3 parts of achyranthis bidentatae, 5-8 parts of eucommia ulmoides, 5-7 parts of mistletoe, 6-9 parts of rhizoma curculiginis, 10-13 parts of herba epimedii and 25-35 parts of folium artemisiae argyi. The Chinese mugwort essence oil perineum stick is convenient to use and carry, simple in operation, safe and comfort and more remarkable in effect after multiple use and has efficacies of nourishing yin and tonifying yang, inhibiting bacterial and protecting the uterus and detoxifying and beautifying.

The invention discloses spring organic fertilizer for cattleya hybrida and a preparation method of the spring organic fertilizer. The spring organic fertilizer is prepared from the following components in parts by mass: 12 to 14 parts of ammonium carbonate, 20 to 22 parts of goose dung, 13 to 15 parts of chicken manure, 20 to 25 parts of calcium superphosphate, 10 to 15 parts of potassium permanganate, 14 to 16 parts of fallen leaves and 200 to 300 parts of water. The spring organic fertilizer for the cattleya hybrida, disclosed by the invention, has the advantages that soil hardening can be avoided, topdressing is not needed after one-time fertilizing and the soil structure can be effectively improved.

The invention provides a small and medium-sized cattleya hybrida cultivation method and application. A mixed substrate obtained by mixing humus soil, coconut shred, tree bark and orchid stone is used as a cultivation substrate adopted by the invention, treatment is performed through combining scientific foliage dressing and exogenous growth regulator application. Application of the small and medium-sized cattleya hybrida cultivation method has the advantages that flowering phase of spring flowering cattleya hybrida group is relatively consistant and the number of flower branches and flowers of each plant is increased remarkably can be guaranteed in the next year after being treated.

The invention discloses R2R3MYB transcription factors that promote anthocyanin formation in two kinds of orchids. The transcription factors are shown in SEQ ID NO: 1 and SEQ ID NO: 2, and the transcription factors are isolated and identified from Cattleya. The corresponding transcription factors can enrich the transgenic material library for the red trait of orchid flowers, which provides strong support for the molecular breeding of orchid red trait flowers in the future.