418 results about "Growth phase" patented technology

New methods for producing tissue engineered constructs and engineered native tissues are disclosed. The methods include producing a tissue engineered construct by growing cells in vitro on a substrate and then decellularizing the construct to produce a decellularized construct consisting largely of extracellular matrix components. The construct can be used immediately or stored until needed. The decellularized construct can be used for further tissue engineering, which may include seeding the construct with cells obtained from the intended recipient of the construct. During any of the growth phases required for production of the construct, the developing construct may be subjected to various tissue engineering steps such as application of mechanical stimuli including pulsatile forces. The methods also include producing an engineered native tissue by harvesting tissue from an animal or human, performing one or more tissue engineering steps on the tissue, and subjecting the tissue to decellularization. The decellularized, engineered native tissue may then be subjected to further tissue engineering steps.

Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with novel vectors, a special selection of hosts, and/or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special vectors are provided which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed.

A system, method, and computer program product to automatically eliminate the distribution information available for reconstruction from a disguised dataset. The invention flattens input numerical values into a substantially uniformly distributed dataset, then maps the uniformly distributed dataset into equivalent data in a target distribution. The invention allows the incremental encryption of new values in an encrypted database while leaving existing encrypted values unchanged. The flattening comprises (1) partitioning, (2) mapping, and (3) saving auxiliary information about the data processing, which is encrypted and not updated. The partitioning is MDL based, and includes a growth phase for dividing a space into fine partitions and a prune phase for merging some partitions together.

A method of producing a polypeptide in fed batch cell culture is provided which involves an initial cell growth phase and a distinct production phase. In the initial growth stage, animal cells having nucleic acid encoding the polypeptide are cultured at a starting osmolality of about 280-330 mOsm in the presence of a concentration of glucose controlled throughout the culturing to be within a range between about 0.01 and 1 g/L. This is followed by a production phase, where the cultured animal cells of the growth phase are inoculated at a cell seed density of at least 1.0×10⁶ cells/mL and the cells are cultured at a starting osmolarity of about 400-600 mOsm in the presence of a concentration of glucose controlled throughout the culturing to be within a range between about 0.01 and 1 g/L. Preferably, the glutamine concentration in the cell culture medium is simultaneously controlled in order to curtail production of lactic acid and ammonia which result from unnecessarily high glutamine concentrations. During the growth phase, production of potentially detrimental metabolic waste products, such as lactic acid, is controlled thereby curtailing the increase of osmolality due to accumulation and neutralization of waste products. Thus, the cell growth can be improved. In the production phase, the cell culture conditions are modified in order to arrest or reduce cell growth and thereby direct nutrient utilization toward production, as opposed to cell growth. Overall, it is intended that the method results in an improvement in specific productivity, reduction in production run times and/or an increase in final product concentration.

The present invention describes methods and processes for the production of proteins, particularly glycoproteins, by animal cell or mammalian cell culture, preferably, but not limited to, fed-batch cell cultures. In one aspect, the methods comprise at least two temperature shifts performed during the culturing period, in which the temperature is lower at the end of the culturing period than at the time of initial cell culture. Throughout their duration, the culturing processes of the invention involving two or more downward shifts in temperature sustain a high viability of the cultured cells, and can yield an increased end titer of protein product, and a high quality of protein product, as determined, e.g., by sialic acid content of the produced protein. In another aspect, the methods comprise the delayed addition of polyanionic compound during the culturing period. The delayed addition of polyanionic compound sustains a high viability of the cultured cells, and can extend the growth phase, delay the onset of the death phase, and arrest the death phase.

Machine executable method of analyzing growth curve data to identify the transition from a baseline phase into a growth phase. Applications of the method include analysis of results from time-dependent monitoring of amplicon synthesis in a nucleic acid amplification reaction to quantify a starting amount of a nucleic acid template in a test sample. The method advantageously simplifies the quantitation by circumventing the need to establish thresholds used for calculating initiation of the growth phase, to calculate derivatives, or to perform linear regression analysis.

The invention discloses an intensive pig farm weanling pig feed and belongs to the field of feeds. The feed is composed of a milk replacer, a weanling feed and a piglet feed. According to the feed, a feed product suitable for different growth phases of a weanling pig are provided according to growth and development characteristics of the different growth phases of the weanling pig so as to promote the development of an intestinal system of the weanling pig and effectively solve the stress reaction appearing in a weaning process of the weanling pig; meanwhile, the utilization rate of the feed is high, the growth speed of the weanling pig can be accelerated, the immunity of the weanling pig can be enhanced and the foundation is laid for the rapid growth of a fattening period. Furthermore, the invention further provides a feeding mode of the feed, namely 6kg of the milk replacer is fed from the 7th day after the weanling pig is born to a 42-45-day age of the weanling pig; 20kg of the weanling feed is fed from the 42-45-day age to a 66-70-day age of the weanling pig; 40kg of the piglet feed is fed from the 66-70-day age to a 94-100-day age. The mode is scientific and reasonable, simple and efficient.

In algae, the conditions for optimal production of biomass are different than the optimal conditions for oil/lipid production. Conventional processes require that both steps be optimized simultaneously which is necessarily sub optimal. The invention provides systems and processes for optimizing each type of production separately and independently, thereby improving overall production of oil, lipids and other useful products. This process is advantageous because it allows the optimization of the individual steps and growth phases in the production of oil from biomass. This allows the use of different feedstocks for various process steps.

The invention discloses a large-scale cultivation method and a quality detection method of cordyceps militaris fruit bodies, comprising the steps of: (1) screening spawn; (2) preparing a rice solid culture medium (40-55 parts of rice and 45-60 parts of nutrient solution in every 100 parts by weight) and killing bacteria; (3) preparing liquid spawn: activating the spawn, inoculating the spawn into a liquid culture medium, conducting static culture for 24 hours and performing suspension culture on a shaking table; (4) culturing fruit bodies: inoculating the liquid spawn on the solid culture medium for dark culture for 6 days at the temperature of 20 DEG C until the surface of the medium is covered by mycelium; placing the well grown mycelium under incandescence with light intensity of 100 to 300 lux, wherein during a primordium growth phase, illumination is performed for 20-24 hours per day at the temperature of 20-24 DEG C; and during a fruit bodies growth phase, illumination is performed for 8-12 hours per day at the temperature of 18-20 DEG C and the fruit bodies are harvested 5 to 8 days after mature. The cordyceps militaris fruit bodies cultured by the method have the advantages of high yield and high content of active ingredients. Batches of cordyceps militaris fruit bodies have fingerprints with a similarity value greater than 0.950, indicating stable and controllable quality.

The invention provides a remote sensing extraction method of agricultural disaster information based on vegetation index time-space statistical characteristics and belongs to the technical field of agricultural disaster information acquisition, aiming at solving the problems that existing agricultural disaster remote sensing monitoring cannot be used for monitoring and evaluating a large-scale region or a long time sequence in the region and a monitoring method has no universality. On the basis of considering that vegetation indexes of different phonological regions, crops and growth phases have difference, an NDVI (Normalized Difference Vegetation Index) of each pixel in the phonological regions with known diseases and an average value NDVIm of the NDVI and a standard difference STD of the same crops are extracted; a relation between the parameters is analyzed by utilizing the statistical characteristics according to NDVI gradation histogram characteristics before and after the disasters and a disaster monitoring model is established; the agricultural disasters are extracted. According to the method provided by the invention, interference factors caused by the growth regions, different crops and growth phases are considered and the precision of a monitoring result is improved. The remote sensing extraction method provided by the invention is used for monitoring the agricultural disasters.

The invention relates to the technical field of forecast and control in insufficient irrigation of crops and discloses control system and method for insufficient irrigation of crops. A data base including data such as water requirement, pan coefficient and water deficit percentage allowed by insufficient irrigation is established for corps in different areas and different growth phases. Evaporation from water surface of evaporation pans and rainfall are acquired by an automatic data acquisition system. Start time and duration of irrigation are calculated according to a formula. Forecast and control in insufficient irrigation are achieved by a controller system. The control method is applicable to forecasting and controlling insufficient irrigation for field crops in arid and semi-arid areas, gardens and protected agriculture, and is applicable to various irrigation methods of spray irrigation, drip irrigation, basin irrigation and the like. The control system and method are widely applicable. The control method is simple and is low in cost. Precision is guaranteed, and automation level is high.

The invention relates to a fertilizer. A organic-inorganic slow-release compound fertilizer special for maize, contains grained inorganic nitrogen, phosphorus and potasium fertilizers, which is characterized in that: the grained inorganic nitrogen, phosphorus and potasium fertilizers are macroaggregate urea, slow-release coated urea, monoammonium phosphate and kalium sulphuricum; and the fertilizer is mixed with an organic grained fertilizer used animal manure, bean cake And straw as raw materials; where the fertilizer contains 18-20by weight of macroaggregate urea, 34-36by weight of slow-release coated urea, 15-17by weight of monoammonium phosphate, 14-16by weight of kalium sulphuricum and 14-15by weight of organic grained fertilizer. The invention can provides a long time nutrient based on the nutritional needs of each growth phase of the maize by once fertilization, can hold and improve soil structure and fertility, and solves the technical problems of convenient preparation, storage, transport and fertilization.

An apparatus for creating therapeutic charge transfer in tissue includes a coil. The coil generates a changing magnetic field to induct an electric field in the tissue exceeding 10 mV/cm when the coil is 5 cm from the tissue. Preferably, the magnetic field has a growth phase and a decay phase and a duration of the growth phase is at least ten times a duration of the decay phase. The apparatus can include a control circuit to control a current fed to the coil. The control circuit includes two subcircuits and a switch for switching between a first of the subcircuits and a second of the subcircuits; preferably, a λ of the second subcircuit is at least ten times a λ of the first subcircuit. To generate the therapeutic effect, the coil should have a duty cycle of at least ten percent.

The invention provides a skeletal age detecting method and device based on depth learning. The method comprises the steps of inputting, wherein X-ray pictures of hands of an examinee and the gender ofthe examinee are input; obtaining, wherein multiple key points corresponding to each skeleton on the hands are obtained from the X-ray pictures; extracting, wherein respective initial pictures of partial skeletons are extracted from the X-ray pictures; correcting, wherein the initial pictures of each skeleton in the partial skeletons are corrected to obtain respective correction pictures of the partial skeletons; primary determining, wherein growth phase information of each skeleton in the partial skeletons is determined, and on the condition that the skeletons do not include carpal bones, the growth phase information of the carpal bones is determined; secondary determining, wherein the skeletal age of the examinee is determined according to the growth phase information of each skeleton in the partial skeletons or the growth phase information of each skeleton in the partial skeletons and the growth phase information of the carpal bones.

An apparatus and method are provided for treating wastewater with alkanes such as butane. An oxygen-containing gas may also be introduced into the wastewater. Butane, because of its relatively high solubility, rapidly dissolves in the wastewater, thereby significantly increasing the heterogeneous microbial community and heterotrophic microbial population. This enhanced microbial population may rapidly absorb and mineralize materials in the wastestream. After an initial growth phase, the organic matter available in the wastewater effluent may be rapidly decreased, thereby reducing the amount of BOD, TDS, sludge and other pollutants. In addition, the use of butane reduces noxious odors associated with municipal wastewater sludges and other types of wastewater.

The invention discloses a fruit tree and Chinese medicinal herb interplanting method. The method includes the steps of planting fruit tree seedlings according to the row spacing of 2-2.5m and plant spacing of 1-1.2m, and planting chrysanthemum, Anemarrhenae and bidentate achyranthes among the fruit tree seedlings; after two to three years, replacing the medicinal herbs planted among the fruit tree seedlings with Isatis tinctoria, honeysuckle and rehmannia; after five years, replacing the medicinal herbs planted among the fruit tree seedlings with pseudo-ginseng, ginseng and Ganoderma lucidum. The problems with interplanting of Chinese medicinal herbs and fruit trees are improved by various methods; different types of medicinal herbs are selected in the different growth phases of the fruit trees, different fertilizing methods and different fertilizers are selected for the different growth phases of the fruit trees, the seeds of Chinese medicinal herbs are specially treated, and the yield of both the fruit trees and the Chinese medicinal herbs is increased and income of fruit growers is increased.

The invention discloses a fermentation method for promoting mythic fungus acid or mythic fungus polysaccharide biology to synthesize. The invention investigates the condition which is needed in the growth phase of mythic fungus cell and product synthesis phase, which respectively investigates the influence of control strategies of pH two phases and oxygen dissolution two phases to mythic fungus acid or mythic fungus polysaccharide biology according to unconsistency of environmental conditions which are needed in the phases of mythic fungus cell growth and production synthesis, and the invention respectively investigates the influence to mythic fungus acid or mythic fungus polysaccharide biology synthesis after control strategies of pH two phases and oxygen dissolution two phases or the fed strategy are mutually coordinated together on the basis of combining with an intermediate fed strategy. The test result shows that mythic fungus cells are obviously prompted to grow no matter respectively adopting a pH value two phases control strategy, an oxygen dissolution concentration two phases control strategy, or coordinating the pH value two phases control strategy and the oxygen dissolution concentration two phases control strategy with the fed strategy together, and the content or yield of mythic fungus acid or mythic fungus polysaccharide are greatly improved.

An ecological technology for treating the nutrients-enriched water polluted by blue alga features that the water hyacinth is cultured in the water by a certain density and then periodically collectedin its growth phase. Its advantages are high effect and low cost.

A method is provided for producing crystalline nanoparticle semiconductor material. The method includes the steps of mixing a precursor in a solvent to form a reaction mixture and subjecting the reaction mixture to microwave dielectric heating at sufficient power to achieve a superheating temperature of the reaction mixture. A growth-phase reaction is permitted to proceed, wherein nanoparticles are formed in the heated reaction mixture. The reaction is then quenched to substantially terminate nanoparticle formation.