72 results about "Inorganic nitrogen" patented technology

The invention discloses a nitrifying bacterium agent suitable for aquarium, fishpond and other aquatic industries, the strain of the nitrifying bacterium agent is Glutamicacter nicotianae TJ-X, the nitrifying bacterium agent is preserved in Guangdong Microbial Culture Collection Center on February 12, 2025, and the preservation number is GDMCC NO.65877. The invention further discloses a preparation method of the nitrifying bacterium agent. The nitrifying bacteria TJ-X strain suitable for the aquarium industry can perform heterotrophic nitrification and aerobic denitrification under the condition that the salt content is 5wt%, under the condition of laboratory pure culture, within 8 hours, the removal rate of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen is 99% or above, the COD degradation rate is 81%, the nitrifying bacteria TJ-X strain is suitable for the environment where organic matter, ammonia nitrogen, nitrite and nitrate coexist, and the nitrifying bacteria TJ-X strain can be applied to the aquarium industry. The microbial agent has the functions of degrading organic matters such as excrement of ornamental fish and fish food, converting organic nitrogen into inorganic nitrogen, and simultaneously degrading inorganic nitrogen such as ammonia nitrogen, nitrite nitrogen and nitrate nitrogen into nitrogen. And the strain is high in biological safety, high in environmental adaptability and high in growth density, can be applied to water industries with high organic matter content and inorganic nitrogen, such as the aquarium industry and aquaculture, and has a wide application prospect.

The invention discloses kluyveromyces marxianus and application of the kluyveromyces marxianus in production of single-cell protein by taking straw as a raw material. The Kluyveromyces marxianus is a ZH-2 bacterial strain which is CGMCC (China General Microbiological Culture Collection Center) No.32295 The strain is obtained from a wild strain in a laboratory adaptive evolution mode, wheat straw and an inorganic nitrogen source can be used as raw materials for efficiently producing single-cell protein, compared with a parent strain, the utilization speed and the utilization rate of biomass, glucose and xylose are remarkably increased, the yield of ZH-2 SCP can reach 0.047 g.g <-1 >, and the yield of ZH-2 SCP can reach 0.045 g.g <-1 >. And the essential amino acid mode basically accords with adult and infant amino acid modes, and can be used as high-quality protein nutrition supply. The Kluyveromyces marxianus ZH-2 strain capable of obtaining single-cell protein by fermenting wheat straw as a raw material is provided from the perspective of utilizing cheap waste resources, so that the production cost of SCP is greatly reduced, and the problem of protein resource shortage is effectively solved.

The invention relates to the technical field of chemical fertilizer preparation, and discloses a special synergistic pesticide fertilizer for a ratoon cane seedling stage, a preparation method and application thereof, the pesticide fertilizer adopts a core-shell layered structure, and comprises a core fertilizer layer, a pesticide layer and a resin coating layer from inside to outside in sequence; the medical fertilizer comprises the following components: 20-40% of an organic fertilizer; 20%-40% of inorganic nitrogen fertilizer; 5%-20% of a medium element fertilizer; 0.1%-2.5% of an insecticide; 0.1%-2.5% of a bactericide; 0.5%-5% of a composite slow release agent; 0.4%-2% of a resin coating material; and the balance of filler. By optimizing a formula and a preparation process, synergistic release of fertilizer nutrients and pesticide active ingredients is realized, nutrient requirements and disease and pest control requirements of ratoon sugarcanes in the seedling stage are met, the budding rate is remarkably increased, the withered seedling rate is reduced, and yield and income are finally increased; the stability and effectiveness of each component in the pesticide fertilizer can be effectively ensured; through precise application, multiple effects of the pesticide fertilizer are exerted to the maximum extent, and a standardized technical scheme is provided for seedling management of ratoon sugarcanes.

The invention provides an organic mineral fertilizer and a preparation method thereof, and belongs to the technical field of fertilizer preparation. The organic mineral fertilizer is prepared from fermented organic matters, an inorganic nitrogen source, a slow-release phosphorus and potassium source, medium trace elements, a slow-release matrix and a functional microbial agent according to a specific ratio. The composition of the fertilizer is adjusted, the slow-release phosphorus and potassium sources are adopted as raw materials, slow release of phosphorus and potassium is achieved through the slow-release effect of the slow-release phosphorus and potassium sources, and the overall slow-release effect of the fertilizer is achieved through the slow-release matrix. According to the present invention, the organic matter and the mineral element are deeply fused by adjusting the process, the prepared fertilizer has characteristics of comprehensive nutrition, slow release, high efficiency, soil improving, and fertilizer utilization rate increasing, the resource utilization of the agricultural waste is achieved, and the green and environmental protection modern agriculture requirements are met.

The invention relates to the field of waste utilization of earthworm processing, in particular to an earthworm source amino acid water-soluble fertilizer and a use method. The method comprises the following steps that in the extraction process of eisenia fetida kinase, residues filtered in the preparation process of crude extract serve as a first raw material, and the first raw material is remaining substances except supernate; the raw material II is a substance filtered by a membrane of an ammonium sulfate-membrane separation extraction method; the raw material I and the raw material II are mixed according to the volume ratio of 3: 1, and then an inorganic nitrogen source and an organic amino acid precursor are obtained, so that basic conditions of the water-soluble fertilizer are met. The process combination is creatively utilized, and no waste is generated. And moreover, no polluted organic solvent is used.

The invention belongs to the technical field of in-situ analysis of water environment interface nutrient elements ammonia nitrogen and nitrate nitrogen, and particularly relates to a zeolite and S110MH mixed adsorption film and a preparation method and application thereof. The zeolite and S110MH mixed adsorption film provided by the invention comprises agar hydrogel and adsorbent particles dispersed in the agar hydrogel, and the adsorbent particles comprise zeolite particles and anion exchange resin particles. The invention provides a preparation method of a zeolite and S110MH mixed adsorption film, functional parameters of the mixed adsorption film and a DGT device installed on the mixed adsorption film, and a testing method of DGT in various environmental media. Experiments show that the DGT device provided with the zeolite and S110MH mixed adsorption film can accurately test the time average concentration or diffusion flux of ammonia nitrogen and nitrate nitrogen at rhizosphere of a water body, sediment or aquatic plants, and reflect the bio-availability, geochemical process and mobility of inorganic nitrogen.

The invention provides an efficient resourceful treatment method for industrial wastewater, and belongs to the technical field of wastewater treatment. The method comprises the following steps: adjusting the pH value of wastewater to be treated to 6.8-7.4, adding a composite reducing agent, reacting to obtain a reaction solution, and filtering and separating the reaction solution to obtain a nitrogen-containing filtrate and filter residues; mixing the nitrogen-containing filtrate, magnesium hydroxide and phosphoric acid, adjusting the pH value to 8.5-9.2, reacting, and centrifuging to obtain a supernatant and a solid matter; and performing post-treatment on the supernate to obtain effluent, and drying the solid matter to obtain the nitrogen fertilizer. According to the method, nitrate nitrogen and nitrite nitrogen in the wastewater are reduced into ammoniacal nitrogen through the composite reducing agent, and then the ammoniacal nitrogen is solidified and crystallized through the nitrogen fixing agent. According to the method, inorganic nitrogen in wastewater is used as a raw material, conversion from nitrate nitrogen to ammoniacal nitrogen is completed by means of a composite reducing agent, efficient fixation of ammoniacal nitrogen is achieved, the situation that nitrogen escapes in the form of nitrogen is avoided, the nitrogen is converted into solid nitrogen fertilizer, and available resources are provided for downstream related enterprises.

The invention belongs to the technical field of biology, and particularly relates to Meyerozyma caribba XX2120, and the preservation number of the Meyerozyma caribba XX2120 is GDMCC No: 67777. The invention further relates to a preparation method of the Meyerozyma caribba XX2120. The strain is obtained by taking saccharomycetes X2120 as a starting strain, carrying out ARTP mutagenesis treatment and screening, has relatively strong acid resistance, relatively high salt stress resistance and certain alcohol resistance and aldehyde resistance, can effectively utilize monosaccharide, disaccharide and trisaccharide, has a wide utilization range on carbohydrate and has a relatively good utilization rate on inorganic nitrogen. Moreover, the strain can be used for producing mycoprotein through liquid fermentation by taking Luzhou-flavor distillers' grains as a raw material, and also can be used for performing solid fermentation on the Luzhou-flavor distillers' grains, so that the true protein content of the distillers' grains is increased, the nutritional value of the distillers' grains is improved, and the recycling of distillers' grain resources is effectively promoted.

This invention provides an electrochemical removal device for inorganic nitrogen in pond aquaculture effluent and its application. The device has an anode and a cathode at its two ends, with multiple sets of anion exchange membranes and cation exchange membranes alternately arranged from the anode to the cathode, forming a concentration chamber and a denitrification chamber in sequence, starting and ending with the concentration chamber. The concentration chamber containing the anode and cathode is referred to as the anode chamber and cathode chamber, respectively. In the denitrification chamber, nitrate, nitrite, and ammonium ions in the pond aquaculture effluent migrate to the concentration chamber, cathode chamber, and anode chamber under electrodialysis, resulting in a rapid decrease in ion concentration in the effluent, which is then directly discharged. The cathode is loaded with a copper-based metal catalyst, which can electrocatalytically reduce nitrate and nitrite nitrogen to nitrogen gas. The anode is loaded with a copper-based oxide or hydroxide catalyst, which can electrocatalytically oxidize ammonia nitrogen to nitrogen gas without the need for active chlorine, thus solving the adverse effect of the lack of chloride ions in pond effluent on ammonia nitrogen conversion.

The invention discloses application of pseudoarthrobacter in synchronous nitrogen and phosphorus removal of low-carbon high-salt mariculture wastewater, the preservation number of the pseudoarthrobacter is CCTCC AB 205501, and the pseudoarthrobacter is preserved in the China Center for Type Culture Collection. Under low-carbon and high-salt conditions, in ammonia nitrogen, nitrate and mixed nitrogen source wastewater, the removal rate of phosphate in 72 hours exceeds 95%, the highest removal rate of ammonia nitrogen can reach 100%, and the highest removal rate of total inorganic nitrogen can reach 98% or above. The efficiency is obviously superior to that of the traditional method, and the treatment time and energy consumption are reduced.

A low-temperature-resistant heterotrophic nitrification and aerobic denitrification bacteria is used for wastewater denitrification method, relates to a kind of biological bacteria for wastewater denitrification method, the bacteria is Pseudomonas umsongensis BK17, belongs to Pseudomonas, is preserved in China typical culture preservation center, and the preservation number is CCTCC M 20242924, and the preservation date is December 27, 2024.The strain is a heterotrophic nitrification-aerobic denitrification bacteria, can efficiently remove inorganic nitrogen pollution in water body, and still has good denitrification performance under low temperature stress, and has strong low temperature tolerance.The strain can efficiently treat nitrogen-containing wastewater at 10 DEG C, and the removal efficiency can reach more than 99%.In the inorganic nitrogen pollution treatment and application of various water bodies such as domestic sewage in cold regions of China, it has great potential.

The present application belongs to the technical field of remote sensing monitoring of water pollution, and is a remote sensing inversion method and system for nitrogen and phosphorus nutrients and chemical oxygen demand in near-shore sea areas. Remote sensing data, reanalysis data and in-situ water quality monitoring data are obtained, and spatio-temporal matching is performed to construct a star-ground spatio-temporal data set. Spatio-temporal information is added, and the selected features are expanded through feature engineering to construct a star-ground spatio-temporal big data set that integrates spatio-temporal features and marine physicochemical properties. A basic model is constructed to determine the input features for predicting inorganic nitrogen, active phosphate and chemical oxygen demand, respectively, to form water quality parameter models, and to train and optimize the inversion models of the water quality parameters to depict the complex nonlinear relationship between nitrogen and phosphorus nutrients and chemical oxygen demand in water bodies and satellite data, marine physicochemical data and spatio-temporal information. The present application realizes long-term, spatially fully covered, and retroactive water quality remote sensing monitoring with high observation frequency.

The present application relates to the field of wastewater treatment, and particularly relates to a method for advanced denitrification of low carbon-nitrogen ratio wastewater based on short-cut nitrification and denitrification and anaerobic ammonia oxidation, which establishes an activated sludge bioaugmentation system by adding biochar immobilized ZB403 strain into an SBR activated sludge system, and domesticates the activated sludge bioaugmentation system by adjusting the component and content of simulated wastewater and optimizing the operation conditions of the bioaugmentation system, and optimizes the biological community structure in the system. The activated sludge bioaugmentation system is used for treating low carbon-nitrogen ratio wastewater, the inorganic nitrogen removal rate is above 99%, the COD removal rate is above 97%, and the sludge yield coefficient Yobs is 0.27-0.29 kgVSS / kgCOD.

The utility model discloses a kind of resourceization pretreatment systems of livestock and poultry manure, including in the raw material pool for storing livestock and poultry manure is sequentially connected with soaking pool, grating pool, sand water separator, screening machine by pipeline, there is grinder in the screen of screening machine, its discharge port is connected with the feed inlet of screening machine, forming the circulation loop of screening and grinding, first fermentation device, second fermentation device and third fermentation device are sequentially connected in the under-screen material discharge end of screening machine. In the utility model, under the joint action of each device, the fermentation period of livestock and poultry manure can be effectively shortened, the fermentation effect of livestock and poultry manure can be effectively improved, and more organic matter in livestock and poultry manure can be converted into organic nitrogen and inorganic nitrogen, so that livestock and poultry manure can be efficiently converted into biogas and fermentation liquid which can be resourcefully utilized, with the advantages of high fermentation efficiency, good fermentation effect, good comprehensive utilization rate, etc., conducive to realizing harmless and resourceful treatment of livestock and poultry manure.

The invention relates to the technical field of soil fertility improvement, and discloses a soil fertility improvement method for supplementing cultivated land soil. The soil fertility improvement method comprises the following steps: S1, applying a base fertilizer I in a wheat season, and dressing a quick-acting inorganic nitrogen fertilizer or a phosphorus-potassium compound fertilizer; s2, after wheat is harvested, straws are separated from the field, and wheat stubbles are ploughed after a decomposition agent is added; s3, base fertilizer II is applied in the soybean season, and quick-acting inorganic nitrogen fertilizer and phosphorus-potassium compound fertilizer are topdressed; s4, after the soybeans are harvested, straw is separated from the field, and stubbles are smashed and returned to the field; wherein in the step S1, the base fertilizer I comprises decomposed manure and a potassium sulfate type compound fertilizer; in the step S2, the base fertilizer II comprises decomposed manure, a quick-acting inorganic nitrogen fertilizer, a water-soluble phosphate fertilizer and a potash fertilizer; in the base fertilizer I and the base fertilizer II, the decomposed manure is prepared by fermenting animal manure, straws, mushroom residues and a complex microbial inoculant. By means of the soil fertility improving method, the nutrient indexes such as soil organic matter and total nitrogen can be obviously improved, and the good soil fertility improving effect is achieved.

The application discloses a strain of Sideropenaeus paramamosain bio-floc high-pond Shajia external sulfur red spirillum HDES1 and application of the strain in purifying inorganic nitrogen and phosphorus in aquaculture water, and belongs to the technical field of microorganisms. The Shajia external sulfur red spirillum HDES1 in the application is screened from a Sideropenaeus paramamosain bio-floc aquaculture high-pond, and has a preservation number of CCTCC NO: M 20191007. It is found through experiments that the strain has a remarkable purifying effect on high-concentration inorganic nitrogen and phosphorus in seawater aquaculture water or tail water, has no adverse effect on cultured Sideropenaeus paramamosain and seawater tilapia, and is helpful to improving the yield of fish and shrimp culture and realizing zero-water-change green ecological culture.

The present application belongs to the field of microalgae biotechnology, microalgae culture regulation and high-value lipid bio-manufacturing, and specifically relates to a method for improving microalgae biomass and eicosapentaenoic acid (EPA) yield by regulating amino acids, which takes microalgae capable of synthesizing EPA as a culture object, adds exogenous amino acids or amino acid combinations to the culture system under non-stress culture conditions containing sufficient inorganic nitrogen source, regulates photosynthesis, growth metabolism and lipid synthesis process of the microalgae, and simultaneously improves microalgae biomass and EPA volumetric yield; by adding specific exogenous amino acids or amino acid combinations under the condition of sufficient nutrition culture, the present application realizes synchronous promotion of microalgae growth and EPA accumulation. Different from traditional methods of relying on stress such as nitrogen deficiency to induce lipid accumulation, the present application can improve microalgae biomass, photosynthetic performance, pigment yield and EPA volumetric yield without significantly inhibiting cell growth and photosynthetic activity.

The invention provides a GLP-1-Fc fusion protein synthesis culture medium which is completely composed of chemical definition components, does not contain complex organic nitrogen sources such as yeast extract powder and peptone, and is mainly composed of a carbon source, an inorganic nitrogen source, vitamins, trace elements, inorganic salt and amino acid. On the basis of an inorganic nitrogen source, high-proportion essential amino acids in target proteins such as Val, Lys, Leu and Phe are directionally supplemented, and sodium citrate is added as a TCA cycle intermediate to strengthen energy metabolism. According to the strategy, the intracellular amino acid self-synthesis energy consumption can be remarkably reduced, and the translation efficiency and metabolic balance are improved; a 5L fermentation tank verification result shows that the final yield of the GLP-1-Fc fusion protein reaches 0.72 mg / mL and is more than two times that of a BSM culture medium, and impure proteins are remarkably reduced; the culture medium disclosed by the invention is controllable in formula, high in repeatability, capable of effectively reducing the metabolic burden and increasing the expression quantity of target protein, and suitable for industrial production of GLP-1 analogues and other high-amino-acid bias proteins.

A method for rapidly preparing amino acid organic fertilizer by using agricultural and forestry wastes, comprising the following steps: 1) preparing agricultural and forestry waste digestion solution; 2) neutralizing and detoxifying; 3) fermenting; 4) hydrolyzing autolysis; and 5) finishing. The present application uses cheap agricultural and forestry wastes such as wood chips and straw chips to prepare organic compost, which is combined with inorganic nitrogen source to be converted into protein, and then is hydrolyzed into organic fertilizer amino acid by using the autolysis property of microorganisms, and is more easily absorbed. The organic fertilizer not only provides ammonia nitrogen in the form of amino acid, but also can improve the survival environment of soil microorganisms, improve soil structure, improve soil fertility, greatly improve product quality, and can be widely popularized.

The invention relates to a method and a system for electrocatalyzing municipal sludge and biological solids. Such methods (and systems thereof) include selecting a sludge source; preparing a slurry, wherein the slurry comprises the sludge source and an electrolyte; adjusting the pH of the slurry, wherein the adjusting the pH of the slurry is such that the slurry has an adjusted pH in a range between about (8) and (14); flowing the slurry through an electrochemical cell, wherein the electrochemical cell includes an anode, a cathode, and a catalyst; applying a potential between the anode and the cathode, wherein applying the potential includes oscillating a cell voltage between the anode and the cathode at an oscillation frequency; breaking carbon bonds with nitrogen and phosphorus in the slurry due to the application of the potential; inorganic nitrogen and inorganic phosphorus are released; and obtaining an electrolyzed sludge, wherein the electrolyzed sludge comprises an electrolyzed solid comprising nitrogen and phosphorus.

The application discloses a method and system for purifying ammonia-nitrogen wastewater by using oyster shell reinforced photosynthetic microorganisms. Seed liquid is obtained by culturing and enriching Rhodopseudomonas palustris, Rhodospirillum profundum, Sulfurspirochaeta shaposhnikovii and Rhodopseudomonas coprophila, and the above photosynthetic microorganisms are used as the core for purifying ammonia-nitrogen wastewater. The photosynthesis of the photosynthetic microorganisms absorbs light energy, converts the light energy into chemical energy, reduces the ammonia-nitrogen in the wastewater into inorganic nitrogen gas by using the chemical energy, and fixes CO2 in the air into biological organic matter, so that the removal of ammonia-nitrogen in the wastewater and the growth and reproduction of the photosynthetic microorganisms are realized. In the application, the photosynthetic microorganisms are used for denitrification, the carbon source input cost in the ammonia-nitrogen wastewater treatment process is greatly reduced, the discarded oyster shell is used as a biological carrier, the pH value of the wastewater can be corrected and CO2 in the water body can be supplemented, and the photosynthetic microorganisms are provided with a favorable growth and reproduction environment and a denitrification environment, and meanwhile, the discarded oyster shell can be treated in a reduced amount.

The invention belongs to the field of microorganisms, and discloses a freshwater aquaculture biological flocculant producing strain and application thereof.The biological flocculant producing strain T-49 can assimilate inorganic nitrogen and convert nitrogen-containing compounds such as ammonia nitrogen in a water body into mycoprotein to form biological floccules capable of being eaten by cultured objects. The bioflocculant producing strain can be prepared into a biological agent or added into fish feed to reduce ammonia nitrogen in water and reduce feed cost. The strain is obtained through screening in a YPG liquid culture medium and shows outstanding water waste flocculation capacity and water ammonia nitrogen removal capacity in flocculation activity determination and ammonia nitrogen removal determination of the strain, and the culture water environment meets the growth condition of T-49. After the T-49 is added, the ammonia nitrogen content of the water body is reduced by 59.60%.

This invention belongs to the field of environmental monitoring technology and discloses a remote sensing inversion method for dissolved inorganic nitrogen and silicate in estuaries based on salinity synergy. The method includes the following steps: S1. During a field survey in the land-sea interaction zone of the estuary, remote sensing reflectance, salinity, and nutrient data are collected simultaneously. The measured hyperspectral reflectance is simulated as the equivalent reflectance of the satellite band using the spectral response function of the target satellite, which is used to construct the training and validation datasets for the model. S2. A nonlinear inversion model of remote sensing reflectance and salinity is established, and a nutrient mixture model of salinity and nutrients is constructed. The training dataset is used for model training. S3. The satellite remote sensing reflectance data of the estuarine area to be predicted is input into the trained nonlinear inversion model and combined with the nutrient mixture model to generate the spatial distribution of DIN concentration and DSi concentration in the estuarine area. This method achieves high-precision remote sensing inversion prediction of DIN concentration and dissolved silicate DSi concentration in the estuarine area.

The application discloses a kind of closed-loop ecological marine system based on multi-species coupling and surface layer circulation, belong to closed-loop ecological marine technical field, it includes: biological coupling processing area, hydrodynamic circulation system;Biological coupling processing area.In the application, through the synergy of multi-nutrient level biological coupling and closed-loop hydrodynamic circulation, efficient self-cleaning and ecological stability to the breeding or simulated seawater body are realized, the flow generator near the water surface can accurately promote the breaking of water surface tension, increase the dissolved oxygen content and the overall ecological biochemical reaction speed and dissolved organic matter, then inorganic nitrogen and phosphorus are rapidly absorbed by feather algae, grape algae and the like, the salt plant root system provides a microbial degradation platform, the salt plant adjusts salinity and absorbs excess nutrients, coral and symbiotic algae form a stable microecological structure, the entire processing chain does not need any chemical agent addition, and the ammonia nitrogen, nitrate and phosphate concentration can be significantly reduced, and dissolved organic carbon is continuously and efficiently removed, which fundamentally avoids the high energy consumption and chemical secondary pollution problems of traditional mechanical filtration.

The invention discloses a method and a device for precisely quantifying soluble organic nitrogen and tracing isotope analysis. The method comprises the following steps: extracting, filtering and removing impurities, namely extracting soluble organic nitrogen and phosphorus in a sediment sample, centrifuging and filtering to remove impurities; separation and purification: carrying out electrodialysis, resin adsorption and anion and cation exchange resin separation and purification on the extracted and filtered soluble organic nitrogen and phosphorus solution to obtain purified soluble organic nitrogen and phosphorus components; concentration and enrichment or catalytic conversion: carrying out nanofiltration on the purified soluble organic nitrogen and phosphorus or carrying out concentration and enrichment on the purified soluble organic nitrogen and phosphorus by a vacuum freezing device, or carrying out catalytic oxidation on the purified soluble organic nitrogen and phosphorus to convert the purified soluble organic nitrogen and phosphorus into inorganic nitrogen and phosphorus; and quantitative and isotope analysis and traceability: carrying out accurate quantitative and isotope analysis on the purified or converted nitrogen and phosphorus, and tracing the source of the soluble organic nitrogen and phosphorus. According to the method, isotope content analysis of dissolved organic nitrogen and phosphorus in the sediment can be rapidly and accurately realized, and the source of the dissolved organic nitrogen and phosphorus can be effectively traced based on a detection result.

This invention belongs to the field of agricultural ecological cultivation and soil ecological regulation technology, and discloses a method for reducing nitrogen and stabilizing maize yield by utilizing the synergistic effect of earthworms and nitrogen application. This invention establishes a cultivation method that can stably achieve nitrogen reduction and stable maize yield under field conditions by combining earthworm inoculation, nitrogen-limited basal application, and dynamic monitoring and regulation of soil inorganic nitrogen. Under the conditions of 220 kg N / ha nitrogen application, earthworm inoculation, and dynamic regulation, maize yield increased by 5.45% compared to the higher nitrogen application treatment, nitrogen fertilizer utilization rate increased by 15 percentage points, and soil inorganic nitrogen accumulation was significantly reduced. This invention overcomes the technical bias that "nitrogen reduction inevitably leads to yield reduction" and provides an operable bio-chemical synergistic solution for reducing fertilizer use and increasing efficiency.

The present application relates to the field of slow-release compound fertilizer preparation, in particular to a green organic compound fertilizer and a preparation method thereof.The green organic compound fertilizer comprises an inner core, the inner core being a fertilizer base particle containing organic matter and inorganic nitrogen, phosphorus and potassium; a coating layer, the coating layer being coated on the surface of the inner core, the coating layer being formed by cross-linking and solidification of a coating liquid and a solidification liquid on the surface of the inner core; the coating liquid comprising an aqueous solution of a lignin-polypeptide graft copolymer and a borate, the lignin-polypeptide graft copolymer being formed by connecting carboxymethylized alkali lignin and an amino-rich polypeptide or a polyaspartic acid derivative through an amide bond; the solidification liquid being an aqueous solution containing a metal ion cross-linking agent; in the coating layer, the lignin-polypeptide graft copolymer and the borate form a borate ester bond, and the lignin-polypeptide graft copolymer and the metal ion cross-linking agent form a metal coordination bond.The present application effectively avoids the secondary pollution problem caused by the degradation of traditional high-molecular chemical resin coating materials to the soil.

This invention relates to a method for treating high-concentration inorganic nitrogen wastewater using a mixed culture of *Paragonimus* strains wg2 and wg1, belonging to the field of resource and environmental microbiology. This invention utilizes the co-culture of strains wg2 and wg1 to treat simulated wastewater, primarily exploring the effect of the mixed strains wg2 and wg1 on NO2 under anoxic conditions. ‑ -N, NO3 ‑ The study investigated the nitrogen removal capacity of nitrite-nitrogen (N), the optimal ratio of mixed bacteria, and the nitrogen removal performance of the mixed bacteria in treating simulated wastewater. Results showed that *Paragonimus* strains wg2 and wg1 acted synergistically in a mixed culture system, effectively addressing both nitrite accumulation and efficient nitrate degradation, demonstrating high nitrogen removal capacity. This study provides important practical guidance for the development of biological nitrogen-removing agents that simultaneously remove nitrite and nitrate.

The invention discloses a fermentation medium for efficiently producing single-stranded DNA and a DNA nanostructure by a phagocytosis method and application of the fermentation medium, and relates to the technical field of biology. The fermentation culture medium is obtained by using ammonium chloride as a single inorganic nitrogen source to replace a nitrogen source (NH4) 2HPO4 in a Riesenberg culture medium. The invention also provides a method for efficiently producing single-stranded DNA (ssDNA) and a DNA nanostructure by a phagocytic grain method, high yield of the ssDNA can be promoted by adopting the fermentation culture medium, a nitrogen source is optimized and screened through the culture medium, and the invention finds that the yield of the single-stranded DNA can be increased by using ammonium chloride as a unique inorganic nitrogen source in the fermentation culture medium for production, and the yield of the single-stranded DNA can be increased by using the ammonium chloride as the unique inorganic nitrogen source in the fermentation culture medium. The yield of single-stranded DNA with triangular, hairpin rectangular, rod-like and quadrilateral DNA structures is increased to different extents by the fermentation culture medium, and a theoretical basis is provided for large-scale production of different types of ssDNA and DNA structures.

This invention relates to the application of a highly efficient halophilic denitrifying bacterium of the genus *Halomonas*. The strain is *Halomonas ramblicola* 5505, a salt-tolerant heterotrophic nitrifying-aerobic denitrifying bacterium, with accession number CGMCC No. 30683. The strain primarily removes ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen from water through assimilation, achieving assimilation rates of 62.68%, 51.08%, and 52.66%, respectively; it can also dissimilate some inorganic nitrogen into nitrogen-containing gases. In simulated nitrogen-containing wastewater, the strain achieved a removal rate of over 90% for ammonia nitrogen and nitrate nitrogen within 12 hours, and increasing the carbon-to-nitrogen ratio completely removed accumulated nitrite nitrogen. This strain effectively solves the problem of inhibition of biological denitrification microorganisms by high-salt nitrogen-containing wastewater. Its nitrogen removal process is green, efficient, and energy-saving, and can be applied to the ecological treatment and restoration of water bodies polluted by high salt and nitrogen.