412 results about "Inorganic nitrogen" patented technology

The invention discloses a microalga photosynthetic aerobic high-density fermentation culture method utilizing an inorganic nitrogen source and an application. The invention relates to a microalga high-density fermentation culture method utilizing the inorganic nitrogen source, wherein photosynthetic aerobic culture is performed in a culture medium in which inorganic nitrogen salt is taken as the inorganic nitrogen source and glucose is taken as an organic carbon source; glucose, inorganic nitrogen salt and other nutrient salts are segmentally replenished according to consumption of the nitrogen source and carbon source of the culture medium and the biomass growth condition; the concentration of the nutrient salts, pH value, ventilatory capacity, light intensity and alga liquid stirring speed are reasonably regulated, so that microalgae can be rapidly proliferated in a short time, the culture time is shortened, high-density growth of the microalgae is guaranteed, rapid growth of the biomass of microalga cells is realized, and high-efficiency absorption and assimilation of inorganic nitrogen are realized. The method disclosed by the invention is suitable for high-density photosynthetic aerobic fermentation production of the microalgae by utilizing the inorganic nitrogen salt as a nitrogen source, and the method lays the foundation of an economic feasibility research on environmental and ecological applications and bioenergy production of the microalgae.

The invention relates to a chemical fertilizer and a production method thereof, in particular to a biological organic inorganic compound fertilizer product and a production method thereof. The biological organic inorganic compound fertilizer product comprises a fertilizer core and a coating in which the fertilizer core is wrapped, wherein the fertilizer core is a particle which is produced by adding humic acid and inorganic nitrogen-phosphorus-potassium raw materials to concentrated waste liquid rich in amino acid, organic matter and other nutrient components, guniting, coating and granulating; the concentrated waste liquid is obtained by treating wastewater of gourmet powder factories; and the coating at least comprises animal oil-fat, starch, bone glue and bacillus components. The product integrates quick, slow and long effects, and integrates organic, inorganic and biological materials. The production method takes the animal oil-fat, the starch and the bone glue which can provide lipid and other nutrients for microorganisms and are easy to degrade as carriers of a bacillus preparation, overcomes the phenomenon that the mixture of the bacillus preparation and other fertilizer components is easy to cause pollution to microbial agents and poison strains.

The invention relates to a method for preparing an algal biological feed. The method comprises the following steps of: preparing complex microbial inoculants from actinomycetes, saccharomycetes, bacillus subtilis, lactobacillus casei and lactobacillus; and fertilizing algas, and under the synergistic effect of a plurality of strains, generating a mass of cellulase type degraded fibers while synthetising mycoprotein by taking full advantage of an inorganic nitrogen source, an organic nitrogen source and saccharides generated through degradation of the fibers. The algal fermented biological feed provided by the invention has more essential amino-acids and higher nutritive value as compared with algas; animals can absorb the feed quickly and the daily gain can be increased by 4-7%; besides, the immunity of the animals is enhanced, the serum alkaline phosphatase activity is improved by 15% and the alanine transaminase activity is increased by 6%; and the breeding effect is quite obvious. In addition, the preparation method provided by the invention has the advantages of low production cost and short period in contrast with the prior art.

The invention relates to a preparation method of abamectin dreg fertilizer; the method solves the problem that: the existing preparation method of the organic fertilizer affects the content of total nitrogen, total phosphorus and total potassium when reducing the content of inorganic nitrogen, phosphorus and potassium in the fertilizer. The preparation method is characterized in that: the preparation method comprises the following steps: (1) dairy manure, slag, maize straw, ammonium hydrogen carbonate and potassium sulphate are uniformly mixed, composted and fermented; after the fermentation is finished, the dairy manure, the inorganic nutrients of which are fixed by microorganisms is obtained; (2) the abamectin dreg is dried to have 40-55% of the water content; (3) the dairy manure, the inorganic nutrients of which are fixed by microorganisms, the abamectin dreg, humic acid and argil are uniformly mixed and are made into particles, thus obtaining the abamectin dreg fertilizer. The method not only can improve the content of total nitrogen, total phosphorus and total potassium in the organic fertilizer, but also can effectively reduce the content of inorganic nitrogen, phosphorus and potassium in the fertilizer simultaneously; therefore, the produced fertilizer has high content of the total nitrogen, the total phosphorus, the total potassium and organic matters.

The invention relates to a fertilizer. A organic-inorganic slow-release compound fertilizer special for maize, contains grained inorganic nitrogen, phosphorus and potasium fertilizers, which is characterized in that: the grained inorganic nitrogen, phosphorus and potasium fertilizers are macroaggregate urea, slow-release coated urea, monoammonium phosphate and kalium sulphuricum; and the fertilizer is mixed with an organic grained fertilizer used animal manure, bean cake And straw as raw materials; where the fertilizer contains 18-20by weight of macroaggregate urea, 34-36by weight of slow-release coated urea, 15-17by weight of monoammonium phosphate, 14-16by weight of kalium sulphuricum and 14-15by weight of organic grained fertilizer. The invention can provides a long time nutrient based on the nutritional needs of each growth phase of the maize by once fertilization, can hold and improve soil structure and fertility, and solves the technical problems of convenient preparation, storage, transport and fertilization.

The invention specifically relates to a multifunctional composite microbial fertilizer and a preparation method thereof, belonging to the technical field of composite microbial fertilizers. The multifunctional composite microbial fertilizer is prepared from the following raw materials: a microbial agent, humic acid, an organic fertilizer, pyrethrum powder, inorganic nitrogen-phosphorus-potassium and trace elements. The multifunctional composite microbial fertilizer can provide crops with desired nutrients, reduces the incidence rate of plant diseases and insect pests, has a high live bacterium number, decreases the usage of pesticides and chemical fertilizers and improves the physical and chemical properties of soil, so the output and quality of the crops are improved. Meanwhile, the preparation method for the multifunctional composite microbial fertilizer is simple, easily practicable and convenient to operate.

The invention relates to a sweet potato skin/sweet potato powder dreg fermented feed and a preparation method thereof. The fermented feed comprises fresh sweet potato skins or sweet potato dregs, an adsorbent, dreg cakes, urea, ammonium sulfate, calcium hydrophosphate, magnesium sulfate, Aspergillus oryzae, Aspergillus niger, Saccharomyces cerevisiae, Candida, and lactobacillus. By means of the oxygen-consuming fermentation of the Aspergillus niger, saccharomycetes and Aspergillus oryzae, all of which are capable of favorably producing various enzyme systems, pectin, crude fibers, protein, starch and other macromolecular substances in fermentation materials are degraded rapidly; in addition, an inorganic nitrogen source is converted into mycoprotein, wherein the lactobacillus produces lactic acid and other active nutrient substances by using micromolecular nutrient substances. The preparation method has the advantage of reducing environment pollution and resource waste caused by difficulty in utilizing the sweet potato skins and the sweet potato powder dregs; after being fed, the fermented feed has the advantages of reducing cultivation cost, increasing cultivation benefits, reducing the cost investment in enzyme preparations and protein feed and increasing the productivity. The fermented feed is a novel resource-utilization type biological feed and has very high economic and social effects.

The invention relates to a high-activity humic acid composite microorganism fertilizer and a preparation method thereof. The high-activity humic acid composite microorganism fertilizer comprises the following components: 20-50% of activated humic acid, 0-20% of an organic material, 0.2-3% of a special-effect composite functional bacterial agent, 0.2-1.8% of a synergist, 30-60% of inorganic nitrogen phosphorus and potassium, 0.2-3% of trace elements and 0.1-0.5% of additives. The high-activity humic acid composite microorganism fertilizer integrates organic and inorganic nutrition and microorganism compounding and pelleting techniques, and the high-activity humic acid composite microorganism fertilizer has the characteristics of low water content, high hardness, availability in mechanical sowing and the like. The high-activity humic acid composite microorganism fertilizer prepared by the preparation method can be used for improving the soil fertility, improving the soil granular structure, fixing nitrogen in the air, decomposing phosphorus and potassium fixed in the soil, activating the micro-ecological environment of plant root soil, promoting growth and development of crops, enhancing the root system, increasing the utilization rate, reducing the use amount, reducing the soil-borne diseases, improving the anti-disease and stress resistance and increasing both production and yield.

The invention discloses a method for producing 1,5-pentanediamine by fermenting a soybean residue hydrolysate. The method comprises the following steps: (1) enzymatically hydrolyzing soybean residuesby use of a composite enzyme to prepare the soybean residue hydrolysate, the composite enzyme comprising protease, cellulase, pectinase and amylase, and preparing a fermentation medium by use of the soybean residue hydrolysate, inorganic nitrogen and an inorganic salt; (2) activating escherichia coli and inoculating the fermentation medium prepared in step (1) with the activated escherichia coli to produce the 1,5-pentanediamine by fermentation. According to the method, the cheap and abundant soybean residues are enzymatically treated to obtain the hydrolysate containing components such as amino acid, peptides and glucose as a raw material for fermentation production of the 1,5-pentanediamine, so that not only are problems about waste treatment of the processing industry solved, but also the production cost of the 1,5-pentanediamine is reduced; the method has a broad development prospect.

The invention relates to a method for fertilizing winter wheat by the mixed basal application of a nitrogen fertilizer and maize straw. The method comprises the following steps of: (1) crushing summer maize straw in fields, returning to the fields completely, and spraying a nitrogen fertilizer solution on the surface of the crushed maize straw uniformly; (2) performing rotary tillage or ploughing; (3) sowing the winter wheat, and applying a phosphorus fertilizer, a potash fertilizer and a trace element fertilizer in a seed manure application mode; and (4) dressing the nitrogen fertilizer onceat the elongation stage of the winter wheat. According to the method, by a field returning mode of spraying the nitrogen fertilizer on the surface of the maize straw, a carbon nitrogen ratio of the straw is regulated, and the nutritional environment which facilitates the growth of straw decomposition microbes is constructed, so that the growth of the microbes is facilitated, the decomposition speed of the straw is improved, inorganic nitrogen of the fertilizer is converted into organic nitrogen, the loss of the nitrogen fertilizer is reduced, and the fertilizer efficiency of the nitrogen fertilizer is improved; and at the middle and late growth stage of the wheat, the amount of the organic nitrogen released by mineralizing the maize straw through the microbes is gradually increased, so that the growth requirement of the wheat at the middle and late growth stage on a nitrogen element is met.

This invention relates to a method for producing orange coloring matters by fermenting monacolin in liquid state, which takes a monascus moldin 9903 as a starter to produce monascus colors with orange as the principle and increases its content and tint, determines a formula of the best fermentation and culture medium, in which, the color content of a fermented fluid with corn flour and (NH4)2SO4 as the carbon and nitrogen sources reaches to 121.7U/mL and its tint is 1.13. The method provides several refining methods incliding: spraying and drying the fermented fluid to get a powder orange product, which is a mixture of multiple color matters showing the orange color tint, the orange content of which reaches to 1030.75U/g and the tint reaches to 1.99, the fluid is extracted to be sprayed and dried to get the content to over 2000U/g and the tint to 2.45 or chromatographed with plate or post to get pure orange pin crystals.

The invention provides a preparation method of gamma-polyglutamic acid, the method uses Bacillus subtilis PGA-7 CCTCC M 206102 as a fermentation strain to carry out the flask-shaking fermentation of a fermentation culture medium so as to prepare the gamma-polyglutamic acid, and the process comprises the slant strain activation, the liquid activation of the strain, the flask-shaking fermentation and the flask-shaking fermented mash extraction. The preparation method adopts the preferential fermentation raw materials combined by a variety of organic nitrogen sources and inorganic nitrogen sources, the highest gamma-PGA production can be 27.3g/l under the best flask-shaking fermentation process conditions, thereby having good industrial application prospect.

The invention discloses a high-temperature cow dung decomposition agent which is prepared from cow dung and a compound microbial agent through fermentation and drying, wherein cow dung particles in the high-temperature cow dung decomposition agent are loaded with compound bacteria of bacillus subtilis, bacillus licheniformis, in-situ composting geobacillus toebii, thermomyces lanuginosus and thermobifida fusca. In addition, the living bacteria count is not lower than 48+/- 100 millions/g, the high-temperature cow dung decomposition agent is 50-650 [mu]m in average particle size, lower than 15% in moisture content and 6.0-8.0 in pH. The compound microbial agent in the decomposition agent generates plenty of cellulase, hemicellulase and protease, so that cow dung is rapidly decomposed into a simple inorganic nitrogen source and a carbon source, further, plant growth is promoted and soil fertility is improved. Fermentation is carried out under the high temperature environment, so that mesophilic plant pathogen growth is inhibited effectively, and cow dung products fermented through the decomposition agent can be directly used as organic fertilizer to be applied to soil.

The invention discloses a method for measuring content of inorganic nitrogen in soil, which comprises the following steps: 1) leaching, namely adding leaching liquor into a soil sample to leach tric nitrogen and ammonium nitrogen in the soil, and filtering to obtain filtered fluid; 2) oxidization, namely adding an oxidizing agent into the filtered fluid obtained in step 1) to oxidize the ammonium nitrogen into tric nitrogen so as to obtain soil total tric nitrogen solution; and 3) performing colorimetric determination with double wavelength on the soil total tric nitrogen solution, and calculating the content of the inorganic nitrogen in the soil according to extinction values obtained by color comparison. The content of inorganic nitrogen in the soil measured by the measuring method has no significant difference with a result measured by a conventional component measurement addition method, and the inorganic nitrogen measured by the measuring method has the recovery rate between 98.8 and 104 percent. The measuring method has the advantages of quickness, accuracy, convenient operation and high efficiency.

The invention belongs to the environmental microorganism field, in particular relating to heterotrophic nitrification aerobic granular sludge and a cultivation method and an application thereof. An inoculated strain of the aerobic granular sludge is stenotrophomonas maltophilia and pseudomonas putida. Activated carbon is adopted to prefix a functional strain culture, ordinary activated sludge is not inoculated, and the heterotrophic nitrification aerobic granular sludge can be cultivated. The aerobic granular sludge can effectively remove ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and a mixture thereof in a water body, can also simultaneously remove CODCr in organic wastewater, is applicable to the treatment of high concentrated organic nitrogen-containing wastewater and inorganic nitrogen-containing wastewater, and does not produce the accumulation of nitrite and nitrate during the denitrification. The application of the aerobic granular sludge for wastewater treatment has simple process and steady denitrification effect.

The invention relates to enrichment of a low temperature-resistant heterotrophic nitrification-aerobic/anoxic denitrification microbial agent and application thereof in wastewater treatment under an anoxic condition. The microbial agent is capable of realizing simultaneous nitrification and denitrification in an aerobic or anoxic environment, in the presence of both ammonia nitrogen and nitrate, with sodium acetate as a unique organic carbon source under a 10 DEG C low-temperature condition; therefore, inorganic nitrogen in wastewater is transformed into gaseous nitrogen and removal of total nitrogen is realized. The low temperature-resistant microbial agent thoroughly realizes maintenance and stabilization of activity of aerobic nitrification and heterotrophic denitrification bacteria under the low-temperature condition, and also has a very high total nitrogen removal rate under an anoxic condition. Besides, the heterotrophic microbial agent grows quickly and lays a good foundation for quick amplification and actual application. When applied to practical town sewage treatment, the microbial agent is capable of efficiently removing total nitrogen in the low-temperature environment under the single anoxic condition; and therefore, the problem that a traditional treating plant hardly reaches the national class A emission standard of total nitrogen in winter is solved and the utilization potentiality of the microbial agent is huge.

The invention relates to a colibacillus capable of generating succinic acid by utilizing synthetic medium pure anaerobic growth. The colibacillus is classified and named as EscherichiacoliBER108 and has the preservation number of CCTCC (China Center for Type Culture Collection) NO.M2012068. The invention further discloses an application of the colibacillus to fermentation production of succinic acid. According to the invention, plasma is utilized to induce colibacillus, a synthetic medium flat screen is utilized to screen out a strain capable of growing rapidly under an anaerobic condition, and under the anaerobic condition, the strain grows by utilizing an inorganic nitrogen source and glucose and accumulates succinic acid; the strain is fermented in a shake flask for 72 hours by utilizing basic magnesium carbonate as a pH regulator; the OD600 of the strain reaches 7.6, the succinic acid yield reaches 11.2g/L, the yield of the succinic acid is increased by nearly three times compared with that of an original strain; and the original strain grows slowly and is low in acid yield under the pure anaerobic synthetic medium condition, so that the mutant strain BER108 has great social significance and economic value.

The invention relates to a cultural method for paracoccus denitrificans and application of the same to purifying aquaculture water, and belongs to the technical field of aquaculture. Various low-value materials such as molasses and waste liquid of monosodium glutamate and production waste which are harmless for aquaculture organisms and the paracoccus denitrificans are used as raw materials, the raw materials are reasonably compounded into liquid culture media, paracoccus denitrifican liquid with high activity and high concentration is produced, the liquid is directly fed into the aquaculture water, an ecological environment of the aquaculture water is repaired, and an excellent water environment beneficial to survival and growth is created for the aquaculture organisms. The culture method and the application have the advantages that the paracoccus denitrificans become effective fast after being used, an inorganic nitrogen removal form and the removal speed can be selected according to an oxygen dissolving condition of the water, contents of harmful substances such as ammonia nitrogen, nitrite and nitrate in the water are remarkably reduced, the quality of the water environment and a survival condition for the aquaculture organisms are improved, the yield of the aquaculture organisms is increased, and the quality of the aquaculture organisms is improved.

The invention discloses microbial fermentation feed. The microbial fermentation feed is prepared from, by weight, 3-5 parts of a complex microbial agent, 680-730 parts of a fermentation raw material,275-325 parts of an energy raw material, 350-400 parts of water and 1-2 parts of brown sugar by mixed fermentation. The complex microbial agent is made by mixing of 5-6 of bacillus subtilis, enterococcus faecalis, plant lactobacillus, bacillus pumilus, clostridium butyricum, bacillus megaterium, bacillus amyloliquefaciens or lactobacillus casei. By fermentation treatment of the fermentation raw material and the energy raw material with the complex fermentation strain, enzymolysis of coarse fibers in the fermentation raw material is realized, organic nitrogen and inorganic nitrogen, which can be hardly used by animals, in the fermentation raw material are converted into bacterial protein, and macromolecular carbohydrates, which cannot be absorbed by the animal, in the energy raw material are converted into absorbable low-molecule carbohydrates.

The invention relates to an anaerobic denitrification phosphorus-accumulating bacteria strain with denitrification and phosphorous removal effects and application thereof. The strain is named as Paenibacillus lautus CL-5, belongs to bright bacillus CL-5 and has the collection number of CCTCC NO: M2012227. The strain is separated and screened from sludge of a double sludge sequencing batch type reactor (DSSBR) when anoxia is ended. The strain is long in growth period and enters the logarithmic phase within 48 hours, the nitrogen and phosphorus removal rate of the strain is respectively 57.5 percent and 50.2 percent in the previous 24 hours, and the nitrogen and phosphorus removal rate in the whole growth period is respectively 72.3 percent and 64.1 percent. The final denitrification product is identified into nitrogen, so that the strain has complete denitrification capacity, and the nitrate nitrogen, nitrite nitrogen and other inorganic nitrogen in the water body can be directly reduced into harmless nitrogen to be discharged out of the water body. The performance detection of the strain proves that the process for treating the nitrogen-containing wastewater by employing the strain is simple, the treatment effect is high-efficiency and stable, NO, N2O and other greenhouse gas are not generated, and air pollution is avoided.

The invention discloses a method for preparing a complete nutrient bioorganic fertilizer from wastes of the biological environment-friendly pig-raising industry and a product, belongs to the technologies of innocent treatment and recycling of agricultural solid wastes, and effectively solves the problem that the wastes of the biological environment-friendly pig-raising industry cannot be treated. The method comprises the following steps of: mixing the wastes of the biological environment-friendly pig-raising industry and inorganic nitrogen fertilizer, phosphate fertilizer and potassic fertilizer in a ratio of (150-180):(10-15):(3-5):(9-12), and performing primary fermentation; and performing secondary fermentation with medium and micro elements (magnesium, iron, boron, molybdenum and manganese) in a ratio of 0.5-1.5 percent (based on dry weight), fully composting, and granulating to obtain the complete nutrient bioorganic fertilizer. The product has the total nitrogen content of 3.62 percent, the total NPK nutrient content of over 9.0 percent and the total medium and micro element content of 2.43 percent. Experimental results show that: after the fertilizer is applied to crops such as wine grapes, Chinese wolfberries, protected cucumbers and protected tomatoes, the yield is increased by 5 to 10 percent compared with that obtained by using simple-substance fertilizers in the same nutrient using amount; moreover, the product quality is obviously improved.

The invention relates to a special-purposed solid fertilizer used for potato seed tuber supplementary irrigation. The special-purposed solid fertilizer is prepared from doped components of inorganic nitrogen fertilizers, inorganic potash fertilizers, inorganic phosphate fertilizers, and chelate medium and micro fertilizers. The total content of main nutrients comprising N+P2O5+K2O+medium and trace elements in the special-purposed fertilizer is no less than 43.1%, wherein nitrogen takes 12.42 to 21.84%, phosphorus pentoxide takes 6.97 to 16.07%, potassium oxide takes 7.1 to 21.52%, calcium takes 0.89 to 1.1%, magnesium takes 0.58 to 0.84%, sulfur takes 0.88 to 8.8%, iron takes 0.10 to 0.14%, manganese takes 0.13 to 0.15%, copper takes 0.13 to 0.17%, zinc takes 0.29 to 0.32%, boron takes 0.07 to 0.1%, and molybdenum takes 0.004 to 0.006%. According to the invention, the production area soil nutrient supplying condition is taken into consideration, nitrogen fertilizers and phosphate fertilizers are increased, and potash fertilizers are appropriately supplemented, such that the loss of medium and micro fertilizers is prevented. The fertilizer provided by the invention has characteristics of comprehensive and balanced nutrients, good specificity, high nutrient concentration, complete ionic state, environment protection, no pollution, low cost, simple preparation technology, and the like. With the fertilizer, potato yield can be improved, potato quality can be improved, agricultural investment can be reduced, and ecological environment can be improved.